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  • 1
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Knochen ; Beckenkammbiopsie ; Spongiosastruktur ; Morphometrie ; Key words Iliac crest bone biopsy ; Microcomputed tomography ; Cancellous bone ; Histomorphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The combined histological and microcomputed analysis of human iliac crest biopsies leads to major advances in our understanding of three-dimensional bone architecture. Microcomputed tomography avoids the time-consuming reconstruction and artifacts of serial sections. Furthermore, its high resolution allows the recording of structural differences as low as 10 mm. Thus, three-dimensional analysis in combination with histological evaluation of cellular dynamics facilitates earlier and easier recording of changes of cancellous bone.
    Notes: Zusammenfassung Es wird die kombinierte Anwendung und quantitative Auswertung 2dimensionaler histologischer Schnitte sowie 2- und 3dimensionaler Mikrotomogramme zur Analyse der Knochenstruktur in Knochenbiopsien beschrieben. Gegenüber dem sehr zeitaufwendigen Verfahren der 3dimensionalen Rekonstruktion aus histologischen Schnitten ist die Mikrotomographie (μCT) schnell genug, um für Routineuntersuchungen in Frage zu kommen. Überdies arbeitet die μCT zerstörungsfrei und vermeidet Schnittartefakte. Art und Ausmaß krankheitsbedingter Spongiosaveränderungen können anhand der 3 D-Tomogramme, dank ihrer hohen Ortsauflösung von rund 10 μm, sehr exakt dargestellt werden. Besonders in Knochenbiopsien mit einer Reduktion der Spongiosastruktur wird das Ausmaß der Strukturveränderungen 3dimensional wesentlich deutlicher als im 2dimensionalen Schnittpräparat. Das Verfahren stellt ein wichtiges Bindeglied zwischen nichtinvasiv erhobenen Strukturdaten und histologischen Veränderungen dar.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 64 (1992), S. A132 
    ISSN: 1432-0584
    Keywords: Hepatitis A virus ; In vitro myelopoiesis ; Long-term bone marrow cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Perturbations of hematopoietic regulation ranging from transient granulocytopenia to rare cases of bone marrow failure are associated with infections due to hepatitis A virus (HAV). In an attempt to elucidate the pathogenetic mechanisms we had previously established that HAV has a direct suppressive effect on human bone marrow progenitors (CFU-GM, -GEMM, BFU-E). These studies were extended to long-term bone marrow cultures (LTBMC): Inoculation of bone marrow mononuclear cells with HAV did not interfere with the establishment of an adherent stromal layer, nor did the inoculation of already established layers cause any morphologically recognizable changes to the stroma. In contrast, a significant and progressive decline of the CFU-GM content in the culture supernatants was demonstrated. HAV antigen was detected by APAAP stain in a subpopulation of stromal cells, and sequential estimations of virus titers in the supernatants provided evidence for viral replication in primary bone marrow cultures. Interferon-gamma and tumor necrosis factor-alpha levels of infected cultures did not differ from those of uninfected controls. These findings argue for a direct suppression of (pre-) CFU-GM by HAV in a model system (LTBMC) lacking an immune defense which would limit viral replication.
    Type of Medium: Electronic Resource
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