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  • 1
    ISSN: 1432-0789
    Keywords: Key words Microbial N ; Immobilization ; Remobilization ; NH4+ fixation ; Exchangeable NH4+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In 1991, field experiments on loess (with winter wheat) and sandy soils (with summer barley) were conducted to study N dynamics in the microbial biomass and non-exchangeable NH4 +. The measurements showed a mass change in microbial N, with a maximum increase of 100 kg N ha–1 30 cm–1 from March to July in the loess soil, and a change for only 1 month (May) in the sandy soil. Plots treated with conventional levels of N fertilizer (213 kg N ha–1 on a loess soil to winter wheat and 130 kg ha–1 on the sandy soil to summer barley), reduced levels of N (83% and 62% of the conventional N application), or no N showed no consistent fertilizer N effect on microbial biomass N. From March to July, non-exchangeable NH4 + in loess soils under winter wheat decreased by 110 kg N ha–1 30 cm–1 in conventionally fertilized plots and by 200 kg N ha–1 30 cm–1 in a plot with no N fertilizer. After harvest, the pool of non-exchangeable NH4 + increased due to increasing mineral N concentrations in the soil.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2307
    Keywords: Purkinje fibres ; Transitional cells ; Working myocardium ; Global ischaemia ; Ultrastructure ; Contraction state
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Contraction bands usually occur in the intramural working myocardium following post-ischaemic reperfusion. In the subendocardium, however, they are found during ischaemia. Thus, we ascertained the contraction states of Purkinje fibres, transitional cells, subendocardial and intramural parts of the working myocardium during 30 min global ischaemia at 25° C. The effects with and without myocardial protection were compared. At the onset of pure ischaemia contraction bands are completely lacking in all cell types. During pure ischaemia contraction bands are found in all subendocardial cell types but not in the intramural working myocardium. A peak of pathological contraction states is found in the intramural working myocardium at the onset (0 min), in the subendocardial working myocardium at 10 min, in the transitional cells and Purkinje fibres at 30 min of pure ischaemia. Histidine-, tryptophan-, ketoglutarate-enriched (HTK) cardioplegia prevents contraction bands completely at the onset of ischaemia and prevents both contraction bands and pathological contraction states during ischaemia almost completely. Striking differences in the physiological contraction states are seen only in the working myocardium: HTK cardioplegia brings about dominance of relaxation during ischaemia. These findings may be due mainly to the effects of global ischaemia on the one hand and to catecholamines, calcium and oxygen on the other.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2307
    Keywords: Purkinje fibres ; Ischaemia tolerance ; Qualitative and quantitative ultrastructure ; Cardioplegia ; Arrhythmias
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary During open heart surgery, reperfusion-induced arrhythmias arising after short periods of ischaemia may originate from subendocardial Purkinje fibres. We investigated the ultrastructure of these fibres during 30 min of global ischaemia at 25° C. The effects both with myocardial protection (HTK cardioplegia) and without it (pure ischaemia) were compared qualitatively and morphometrically. After 30 min pure ischaemia overcontraction of sarcomeres, hypercontraction and contraction bands, together with considerable changes in organelles, predominate over cellular oedema. In Purkinje fibres, both cellular and mitochondrial swelling were significantly increased within this 30-min time period from the onset of pure ischaemia. In contrast, following HTK cardioplegia and 30 min ischaemia, cellular and mitochondrial swelling remain moderate and over-contractions are almost entirely lacking. This means that despite remarkable differences between pure ischaemia and HTK cardioplegia in the degree of protection attained it is clear that, compared with the working myocardium, subendocardial Purkinje fibres do not display a higher resistance to early global ischaemia. Further investigations of this sensitivity of Purkinje fibres to global ischaemia and certain drugs may bring about new insights into myocardial protection and pharmacotherapy of arrhythmias.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0789
    Keywords: Microbial N ; Immobilization ; Remobilization ; NH inf4 sup+ fixation ; Exchangeable NH inf4 sup+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In 1991, field experiments on loess (with winter wheat) and sandy soils (with summer barley) were conducted to study N dynamics in the microbial biomass and non-exchangeable NH inf4 sup+ . The measurements showed a mass change in microbial N, with a maximum increase of 100 kg N ha-1 30 cm-1 from March to July in the loess soil, and a change for only 1 month (May) in the sandy soil. Plots treated with conventional levels of N fertilizer (213 kg N ha-1 on a loess soil to winter wheat and 130 kg ha-1 on the sandy soil to summer barley), reduced levels of N (83% and 62% of the conventional N application), or no N showed no consistent fertilizer N effect on microbial biomass N. From March to July, non-exchangeable NH inf4 sup+ in loess soils under winter wheat decreased by 110 kg N ha-1 30 cm-1 in conventionally fertilized plots and by 200 kg N ha-1 30 cm-1 in a plot with no N fertilizer. After harvest, the pool of non-exchangeable NH inf4 sup+ increased due to increasing mineral N concentrations in the soil.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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