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  • 1
    Electronic Resource
    Electronic Resource
    Untersuchung der DNS-abhängigen RNS-Polymerase aus menschlichen Leukocyten in einem einfachen zellfreien System (1973)
    Springer
    Journal of molecular medicine 51 (1973), S. 730-734 
    Details
    ISSN: 1432-1440
    Keywords: Leukocytes ; Leukemia ; RNA-polymerase ; RNA-metabolism ; Leukocyten ; Leukämie ; RNS-Polymerase ; RNS-Stoffwechsel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lymphocyten und Leukocyten (Lymphocyten + Granulocyten) werden aus 10–40 ml heparinisiertem Venenblut isoliert, homogenisiert und 15 min bei 1000 g zentrifugiert. In dem aus Zellkernen und Kerntrümmern bestehenden Sediment lassen sich nach Resuspension ca. 70% der DNA-abhängigen RNS-Polymerase-Aktivität des Homogenats nachweisen. Die Reaktion ist von zugesetzter DNS und der Gegenwart aller vier Ribonucleosid-Triphosphate abhängig. Bei chronisch lymphatischer Leukämie, chronisch myeloischer Leukämie und Morbus Hodgkin findet sich unter diesen Bedingungen eine höhere spezifische Aktivität der DNS-abhängigen RNS-Polymerase als bei Normalpersonen.
    Notes: Summary Lymphocytes and leukocytes (lymphocytes + granulocytes), isolated from 10–40 ml of heparinized venous blood, are homogenized, and centrifuged for 15 min at 1000 g. The resuspended pellet, consisting of nuclei and nuclear debris, exhibits ca. 70% of the DNA-depenent RNA polymerase activity of the homogenate. The reaction depends on added DNA template and the presence of all four ribonucleoside triphosphates. In chronic lymphatic leukaemia, chronic myelocytic leukemia, and Hodgkin's disease, the activity of the DNA-dependent RNA polymerase is higher than in normal controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468365
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  • 2
    Electronic Resource
    Electronic Resource
    DNS-abhängige RNS-Polymerasen in menschlichen Leukocyten (1975)
    Springer
    Journal of molecular medicine 53 (1975), S. 311-316 
    Details
    ISSN: 1432-1440
    Keywords: DNA-dependent RNA polymerases ; leukemia ; prognostic factors ; DNS-abhängige RNS-Polymerasen ; Leukämie ; prognostische Kriterien
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die spezifischen Aktivitäten der DNS-abhängigen RNS-Polymerasen A und B wurden in von exogener Matrizen-DNS abhängiger Form bei verschiedenen Hämoblastosen bestimmt. Die Aktivitätsbestimmungen erfolgten in Kernhomogenaten isolierter mononucleärer oder segmentkerniger Leukocyten. Eine signifikante Erhöhung der Polymeraseaktivitäten A und B fand sich in den Kernhomogenaten mononucleärer Zellen bei akuter myeloischer Leukämie, während diese bei chronisch-myeloischer Leukämie (signifikant) und chronisch-lymphatischer Leukämie (nicht signifikant) erniedrigt waren. Unter cytostatischer Therapie findet sich eine Angleichung der Polymeraseaktivitäten an den Normbereich. Hiermit ergeben sich möglicherweise neue Kriterien zur Verlaufsbeurteilung von Hämoblastosen unter einer Polychemotherapie.
    Notes: Summary Specific Activities of DNA-dependent RNA polymerases A and B have been determined in nuclei from leukocytes in acute and chronic leukemia. Enzyme activities, dependent on exogenous DNA template, were determined in homogenates of nuclei from isolated mononuclear cells or from isolated granulocytes. Activities of polymerases A and B have been found significantly elevated in homogenates of nuclei from mononuclear cells in acute myelocytic leukemia, while they were found subnormal in corresponding cell fractions from chronic myelocytic leukemia and chronic lymphatic leukemia. During cytostatic treatment polymerase activities were approaching normal values. The prognostic relevance of these data for the course of human leukemia is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01469057
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  • 3
    Electronic Resource
    Electronic Resource
    High-dose chemotherapy and autologous hematopoieticstem cell transplantation in patients with second primary malignancies (1997)
    Springer
    Hematology and cell therapy 39 (1997), S. 79-83 
    Details
    ISSN: 1279-8509
    Keywords: Autologous bone marrow transplantation ; Autologous peripheral blood stem cell transplantation ; High-dose chemotherapy ; Second primary neoplasms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We treated 500 patients with high-dose chemotherapy and autologous bone marrow or autologous peripheral blood stem cell transplantation. Treated conditions included leukemia, lymphoma, breast cancer, lung cancer, germ-cell carcinoma, and other solid tumors. 10/500 (2%) of patients were treated for a second malignancy diagnosed 12 months to 25 years after their initial neoplasm. Four of these ten patients are in complete remission (CR) of both malignancies at a median follow-up of 29+ months after high-dose chemotherapy and autotransplantation. None of these patients would have been eligible for high-dose chemotherapy and autotransplantation by conventional selection criteria which usually exclude patients with a history of prior malignancies. Conclusion. Conventional exclusion criteria for high-dose chemotherapy and autotransplantation may not adequately reflect the prognosis of patients with second or secondary malignancies treated with this therapeutic modality. High-dose chemotherapy and autologous hematopoietic stem cell transplantation may be of true benfit in selected cases of secondary malignancies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00282-997-0079-3
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  • 4
    Electronic Resource
    Electronic Resource
    Leukocytoclastic vasculitis and long-term remission in a patient with secondary AML and post-remission treatment with low-dose interleukin-2 (1995)
    Springer
    Annals of hematology 70 (1995), S. 227-230 
    Details
    ISSN: 1432-0584
    Keywords: Interleukin-2 ; Acute myeloid leukemia ; Leukocytoclastic vasculitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Interleukin-2 (IL-2) has been licensed for the treatment of renal cell carcinoma and is currently being evaluated as a therapeutic agent in hematological malignancies. It is associated with a variety of side effects due to induction of a nonspecific inflammatory response. However, phenomena of autoimmunity have also been reported. Here we describe a patient with secondary acute myeloid leukemia who developed a leukocytoclastic vasculitis during long-term post-remission treatment with very low doses of IL-2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01700380
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  • 5
    Electronic Resource
    Electronic Resource
    Lymphokine activated killer cells (1989)
    Springer
    Annals of hematology 59 (1989), S. 375-384 
    Details
    ISSN: 1432-0584
    Keywords: Lymphokine activated killer cells ; Interleukin-2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Various subpopulations of human leukocytes may be induced by lymphokines to exert cytotoxic activity. In man major histocompatibility complex non-restricted tumor cell lysis by interleukin-2 (IL-2) induced peripheral blood lymphocytes is attributed mainly to natural killer cells. These T cell receptor negative large granular lymphocytes are called lymphokine activated killer (LAK) cells. In order to explore the potential of LAK cells in tumor therapy, several clinical studies have been conducted, using IL-2 alone or in combination with ex vivo IL-2-activated peripheral blood lymphocytes. Objective responses have reproducibly been achieved only in renal cell carcinoma and malignant melanoma and were associated with considerable toxicity. In view of restricted efficacy and increasing doubts as to whether LAK cells indeed account for the in vivo observed responses, more recent strategies focus on tumor antigen specific cytotoxic T cells or tumor infiltrating lymphocytes. Successful translation of this approach into clinical practice, however, may be dependend on some basic problems of tumor immunology to be solved which were thought to be by-passed by the LAK cell approach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00321208
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  • 6
    Electronic Resource
    Electronic Resource
    Early-onset secondary malignancies after high-dose chemotherapy and autologous hematopoietic stem cell transplantation (1997)
    Springer
    Annals of hematology 74 (1997), S. 73-77 
    Details
    ISSN: 1432-0584
    Keywords: Key words Autologous bone marrow transplantation ; Autologous peripheral blood stem cell transplantation ; High-dose chemotherapy ; Second and secondary primary neoplasms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We treated 500 patients with high-dose chemotherapy (HDC) and autologous bone marrow (ABMT) or autologous peripheral blood stem cell transplantation (PBSCT). Treated conditions included leukemia, lymphomas, breast cancer, lung cancer, germ-cell carcinomas, and other solid tumors. In order to assess relapse of primary malignancy or occurrence of new neoplasms, routine screening after ABMT or PBPCT was performed at regular and close intervals. With a total follow-up of 1358 person-years and a median follow-up of 34 months (range 9–91), 10/500 (2%) patients developed second malignancies after PBSCT or ABMT; i.e., one new cancer occurred every 136 person-years. All malignancies were detected at routine follow-up examinations; and 7/10 diagnoses were made in an asymptomatic phase; 6/10 neoplasms were amenable to complete surgical resection, five of which remain in CR at a median of 23+ months after autotransplantation. We conclude that regular and close follow-up examination of patients after autologous hematopoietic stem cell transplantation may be beneficial, since successful treatment of second malignancies is possible in selected cases after early detection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002770050260
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