ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The basic fibroblast growth factor (bFGF) is known to proliferate and maintain the adult stem cells. The human mesenchymal stem cells (hMSCs) are very homogenous and constitute of euchromatin nuclei in 10% fetal bovine serum (FBS) and induce the heterochomatin nuclei by bone morphogenetic protein.In order to further investigate other growth factor, other cell types like bFGF, keratinocytes, dermal fibroblasts and endothelial cells, the cellular and molecular analyses are performed in vitro assay systems.The various doses of bFGF (0.25–25 μg/ml) significantly proliferate the hMSCs in time-dependent manner in s serum-free medium. The ultra-structure by electron microscopy revealed the very small, round and immature both cytoplasmic and nuclear structure resembling the 10% FBS-cultured cells. The hMSCs and bFGF and other cells are co-cultured in 8 μm-pore chamber systems for 16-hour incubation. The hMSCs successfully migrate through the pore when keratinocytes, dermal fibroblasts, endothelial cells or bFGF (345.0 ± 68.86, 63.8 ± 16.50, 36.2 ± 9.60, 12.0 ± 4.89, p 〈 0.01) are placed lower chamber. The clear cellular attachment between hMSCs and keratinocytes is observed in the monolayer co-culture by electron microscopy.The hMSCs are stimulated by bFGF and interact with other cell types such as keratinocytes, dermal fibroblasts and endothelial cells in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2005.130116ak.x
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