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  • 1
    Digitale Medien
    Digitale Medien
    Molecular analysis and expression of a Borrelia burgdorferi gene encoding a 22kDa protein (pC) in Escherichia coli (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: We describe the cloning and expression of the pc gene which encodes a major immunodominant protein of Borrelia burgdorferi, the causative agent of Lyme borreliosis. The pC protein was purified from lysates of B. burgdorferi strain PKo. After tryptic digestion of the pC protein the resulting oligopeptides were applied to a gas-phase sequenator. Thus partial amino acid sequences were obtained. The deduced oligonucleotides were used as hybridization probes. After Southern blotting a reactive band in the 3 kb range of PstI-digested genomic DNA was detected. The insertion of these fragments into pUC vectors finally resulted in pc-positive Escherichia coli clones. The gene (encoding a protein with 212 amino acids) was expressed in E. coli with varying deletions at the 5′ end. A sequence comparison with other outer membrane proteins of B. burgdorferi indicates a processing of pC that is similar to that of lipoproteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01495.x
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  • 2
    Digitale Medien
    Digitale Medien
    Purification of the Borrelia burgdorferi flagellum by use of a monoclonal antibody
    Amsterdam : Elsevier
    Molecular and Cellular Probes 4 (1990), S. 451-462 
    Details
    ISSN: 0890-8508
    Schlagwort(e): Borrelia burgdorferi ; affinity chromatography ; antigen-specific ELISA ; flagella antigen ; monoclonal antibody
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0890-8508(90)90004-J
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  • 3
    Digitale Medien
    Digitale Medien
    Borrelia burgdorferi myositis: report of eight patients (1993)
    Springer
    Journal of neurology 240 (1993), S. 278-283 
    Details
    ISSN: 1432-1459
    Schlagwort(e): Borrelia burgdorferi ; Lyme disease ; Myositis ; Immunohistology ; Culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Myositis is a rare manifestation of Lyme disease of unknown pathogenesis. This study describes the course of disease in eight patients with Lyme disease, aged 37–70 years, all of whom were suffering from histologically proven myositis. The Clnical, electrophysiological, and myopathological findings are reported. One patient showed signs and symptoms of myositis of all limbs. In six patients myositis was localized in the vicinity of skin lesions, arthritis or neuropathy caused byBorrelia burgdorferi. In another patient suffering from pronounced muscle weakness of the legs and cardiac arrest, inflammation of the myocardium, the conducting system and skeletal muscles was revealed at autopsy. Muscle biopsy revealed lymphoplasmocellular infiltrates combined with few fibre degenerations in three patients. The lymphoplasmocellular infiltrates were found predominantly in the vicinity of small vessels. Several spirochetes were stained in six of seven muscle biopsy samples by means of the immunogold-silver technique. Culturing ofB. Burgdorferi from the muscle biopsy samples was, however, unsuccessful. Antibiotic treatment succeeded in curing the myositis in four of six patients. In one patients signs and symptoms improved. One patient died from cardiac arrest caused by myocarditis and Guillain-Barré syndrome. The outcome is unknown in one patient. Clinical and myopathological findings indicate that Lyme myositis can be caused either by local spreading ofB. burgdorferi or an unknown antigen or toxin from adjacent tissues or haematogenously.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00838161
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  • 4
    Digitale Medien
    Digitale Medien
    Lyme arthritis: Clinical features, serological, and radiographic findings of cases in Germany (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 206-215 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Lyme arthritis ; Lyme disease ; Tickborne spirochetosis ; Borrelia burgdorferi ; Stomoxys calcitrans
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The clinical manifestations, serological data, and radiographic findings of ten cases of Lyme arthritis in Germany are summarized. Qualitative assessment shows that the characteristics of the disease in Germany do not differ fundamentally from those reported in the USA. However, since a serological test for antibodies to the causative spirochete is now available, the great variety of the clinical features of Lyme arthritis can be described more precisely. The cases of chronic Lyme arthritis without prior erythma chronicum migrans, hitherto the most important diagnostic hallmark of the disease, may have been underestimated. One of the cases reported provides evidence that the disease was transmitted via a fly bite. Radiographic abnormalities consisting of marked juxta-articular osteoporosis and osseous erosions were found in two patients with chronic arthritis. Three patients were treated with high-dose intravenous penicillin, two did not respond to the therapy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01711648
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  • 5
    Digitale Medien
    Digitale Medien
    Persistent atrioventricular block in Lyme borreliosis (1990)
    Springer
    Journal of molecular medicine 68 (1990), S. 431-435 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Lyme borreliosis ; Borrelia burgdorferi spirochete ; Lyme carditis ; Atrioventricular block
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Cardiac manifestations are reported in 0.3%–4.0% of European patients withBorrelia burgdorferi (B.b.) infection. Usually symptoms disappear within 6 weeks. We report a case with persistent impairment of atrioventricular (AV) conduction. Diagnosis was confirmed by demonstration of IgM antibodies and increase of IgG antibody titers against B.b. in serum, by isolation of the spirochete from skin biopsy material and by the typical clinical combination of erythema migrans, Bannwarth syndrome (meningoradiculitis), and complete heart block. Despite immediate antibiotic therapy with ceftriaxone, first degree AV block and second degree block Wenckebach with atrial pacing at 100 beats/minute persisted for 2 years. We conclude, that Lyme carditis can cause long-standing or irreversible AV conduction defects despite adequate and early antimicrobial therapy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01648587
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  • 6
    Digitale Medien
    Digitale Medien
    Aktinomykose der haut bei HIV-infektion (1988)
    Springer
    Journal of molecular medicine 66 (1988), S. 651-653 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Actinomycosis ; AIDS-related Complex (ARC) ; Drug Abuse ; HIV-Infection ; Lymphadenopathy (LAS) ; Opportunistic Infections
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A 29-year-old woman with HIV-infection developed disseminated abscesses of the skin, identified as an infection with Actinomyces israelii. To our knowledge, actinomycosis associated with HIV-infection (AIDS-related complex) has been reported only once previously.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01728809
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  • 7
    Digitale Medien
    Digitale Medien
    Dietary docosahexaenoic acid is retroconverted in man to eicosapentaenoic acid, which can be quickly transformed to prostaglandin I"3
    Amsterdam : Elsevier
    Prostaglandins 34 (1987), S. 367-375 
    Details
    ISSN: 0090-6980
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0090-6980(87)90082-7
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  • 8
    Digitale Medien
    Digitale Medien
    Molecular characterization of the p83/100 proteins of variousBorrelia burgdorferi sensu lato strains (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 305-306 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Conclusions As in OspA-serotyping experiments, theB garinii group (OspA-sterotype 3–7) showed highest diversity within this internal fragment of p83/100, whereas theB. afzelii group (OspA-type 2) and theB. burgdorferi sensu stricto group (OspA-type 1) were nearly identical. Determination of the size of the PCR products as well as restriction fragment length polymorphism analysis (DraI) can be used for classification into the three species ofB. burgdorferi sensu lato. Since p83/100 is chromosomally encoded, this protein might be a more stable marker for classification than the plasmid-encoded OspA. In contrast to the flagellin gene a subclassification of theB. garinii group is possible due to the diversity of the p83/100 internal fragment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01919522
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  • 9
    Digitale Medien
    Digitale Medien
    Recombinant immunoblot in the serodiagnosis of Lyme borreliosis (1993)
    Springer
    Medical microbiology and immunology 182 (1993), S. 255-270 
    Details
    ISSN: 1432-1831
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A recombinant immunoblot was developed for detection of IgM and IgG antibodies in patients with Lyme borreliosis. The recombinant antigens were the chromosomal-encodedBorrelia burgdorferi proteins p100, the flagellin and an internal flagellin fragment thereof as well as the plasmid-encoded outer surface proteins A (OspA) and C (OspC). A panel of 144 sera from patients with Lyme borreliosis (erythema migrans,n = 31; neuroborreliosis state II,n = 60; Lyme arthritis,n = 24 and acrodermatitis chronica atrophicans,n =19) have been investigated and the results have been compared to the immunofluorescence absorption test (IFA-ABS) and to two different enzyme-linked immunosorbent assays [the flagellin ELISA and a newly developed ELISA (OGP-ELISA)]. The two ELISAs were comparable in sensitivity, whereas the IFA-ABS was less sensitive for IgM antibody but equally sensitive for IgG antibody detection. Immunoblot analysis revealed that IgG antibodies are mainly reactive with p 100 and the internal flagellin fragment (sensitivity 51% and 32%, respectively) and rarely with OspC (14%). All patients with late Lyme borreliosis had IgG antibodies against the p100. IgM antibodies were predominantly directed against OspC (43%) and in a lower extent against the internal flagellin fragment and p100 (15% and 13%, respectively). The complete flagellin was not useful due to a high number of unspecific reactions with control sera and the OspA was only exceptionally reactive in Lyme borreliosis patients. The sensitivity of IgM antibody detection could be increased in cases with early Lyme borreliosis from 46% to 65% when the OspC blot was performed in addition to the flagellin ELISA, or from 56% to 65% when performed in addition to the OGP-ELISA. The recombinant blot is, therefore, a valuable diagnostic test to increase sensitivity of early antibody detection and is regarded as a valuable confirmatory test also in late disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00579624
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  • 10
    Digitale Medien
    Digitale Medien
    Genetic heterogenity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi (1993)
    Springer
    Medical microbiology and immunology 182 (1993), S. 37-50 
    Details
    ISSN: 1432-1831
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The ospC gene coding for the outer surface protein OspC and the fla gene coding for the flagellin have been investigated in three different Borrelia burgdorferi sensu lato strains. These strains (the North American strain B31 and the European strains PKo and PBi) derive from various biological sources (lxodes dammini, human skin and human CSF) and belong to three different B. burgdorferi OspA serotypes and genospecies (OspA serotype 1, B. burgdorferi sensu stricto; OspA serotype 2, group VS461 and OspA serotype 4, B. garinii, respectively). The ospC and fla genes of the respective strains have been amplified by polymerase chain reaction, cloned in pUC8 and sequenced. The fla as well as the ospC genes were different among the three strains investigated. In general the fla genes are more conserved than the ospC genes. The fla genes have the same length of 1008 nucleotides coding for proteins of 336 amino acids, whereas the ospC genes differ in length. The ospC genes of strains B31, PKo and PBi have 630, 636 and 621 nucleotides encoding proteins of 210, 212 and 207 amino acids, respecctively. The ospC genes exhibit sequence identities between 70% and 74% among each other, sequence identities of the fla genes are in the range 96–97%. The ospC genes could be expressed in Escherichia coli to obtain proteins with and without leader peptides. The expression of the fla gene and an internal gene fragment resulted in the complete flagellin protein and a truncated protein (amino acids 129–251). The different ospC and fla gene products were immunoreactive with monoclonal antibodies and human sera and, thus, enlarge the spectrum of recombinant antigens to improve antibody detection in patients with Lyme borreliosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00195949
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