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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of carbonic anhydrase in mixed tumours and adenomas of sweat and sebaceous glands (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 14 (1987), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Immunohistochemical distribution of carbonic anhydrase II (CA) in mixed tumours and adenomas of sweat gland origin and in sebaceous adenomas was demonstrated by the PAP method. Normal sweat glands, both eccrine and apocrine, clear cells of the secretory coils, and ductal epithelial cells all showed conspicuous staining for CA, and sebaceous glands were also positive. Mixed tumours of the skin indicated strongly positive staining for CA in the luminal cells of tubular and duct-like or cystic structures, while most of the other tumour cells were negative. In solid or massive foci, CA positive cells were found scattered among the cellular mass. Sebaceous adenomas were usually moderately positive for CA throughout the tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1987.tb00501.x
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  • 2
    Electronic Resource
    Electronic Resource
    Resonance Raman characterization of highly reduced iron octaethylporphyrin: [Fe(OEP)]n- (n = 0, 1, and 2) (1987)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 109 (1987), S. 180-184 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00235a028
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  • 3
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization by monoclonal antibody of human epidermal growth factor in mixed tumours of the skin (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 14 (1987), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Immunohistochemical distribution of human epidermal growth factor (hEGF) was described in 17 cases of mixed tumour of the skin with monoclonal antibody. In normal sweat glands, epithelial cells in the secretory portion and in the transitional area between secretory portion and duct showed prominent staining for hEGF. In the salivary pleomorphic adenoma type of mixed tumour of the skin, luminal tumour cells of tubular and duct-like structures gave a very characteristic hEGF staining reaction. The tumour cells showing strong staining for hEGF were scattered throughout the solid foci in this type of mixed tumour. Tubular epithelial cells in the clear cell adenoma type also displayed a positive hEGF reaction. And apocrine mixed tumours strong staining for hEGF occurred on the apical side of tubular and ductal tumour cells. In view of the immunohistochemical staining patterns for hEGF, the histologic origin of mixed tumours of the skin is suggested to be cells in the secretory portion and (hose in the transitional portion between secretory portion and duct of the sweat gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1987.tb01534.x
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  • 4
    Electronic Resource
    Electronic Resource
    Calcifying epithelioma of Malherbe with ossification. Special reference to lectin binding and immunohistochemistry of ossified sites (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 14 (1987), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Lectin binding sites and immunohistochemically detectable fibronectin and carbonic anhydrase II (CA II) were examined in induced bone structure of calcifying epithelioma of Malherbe. Pathological features of ossification were particularly evident in the border zone between tumor epithelium and stroma, and epithelial foci adjacent to sites of bone formation were conspicuously basophilic. The use of lectin binding in these foci coincided with the basophilic epithelial zones: Con A, RCA-1, PNA, SBA, and WGA bound strongly, indicating thepresence of glucose (Glu), mannose (Man), galactose (Gal), N-acetyl-D-galactosamine (GalNAC), and N-acetyl-D-glucosamine (GlcNAC). Fibronectin was also detected in the same epithclial-mesenchymal interacting layers as the positive lectin-binding sites. Staining for CA II was strongly positive in giant cells and in epithelial zones in the bone-inducting areas. The bond induction mechanism in the stromal tissue of calicifying epithelioma of Malherbe may initially involve the action CA II in the epithelial-mesenchymal interacting zone, which then brings about ossification of the matrix which is fibronectin-positive and rich in lectin-binding sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1987.tb00494.x
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  • 5
    Electronic Resource
    Electronic Resource
    Monoclonal antibody-detected vimentin distribution in pleomorphic adenomas of salivary glands (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 17 (1988), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To evaluate the participation of myoepithelial components in pleomorphic adenomas, an immunohistochemical study was carried out using monoclonal antibodies to vimentin. Of a total of 80 cases, 50 tumors gave positive staining, 5 tumors very slight, and 25 tumors negative staining for vimentin. Localization patterns for vimentin were divided into 3 classes: 1) vimentin staining in fibrous stromal tissue; 2) variable intensities of vimentin staining were found in the outer layers of tumor cells in tubuloductal structures (some of which were spindle cells connected to modified myoepithelial cells which also gave variable vimentin staining); and 3) modified myoepithelial cells and chondroidlike cells displayed strongly positive staining for vimentin. Typical histologic features of pleomorphic ademomas, i.e., tubulo-ductal or duct-like structures were characterized by positive vimentin staining in outer tumor cells and by a positive keratin reaction in the luminal tumor cells. In tumors devoid of stromal connective tissues and the near absence of well-developed, or modified myoepithelial cells, vimentin staining was absent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1988.tb01548.x
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  • 6
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of intermediate filament proteins in calcifying epithelial odontogenic tumors (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 17 (1988), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Three calcifying epithelial odontogenic tumors (CEOT) were examined immunohistochemically. The localization of intermediate filaments was characterized through the use of polyclonal anti-keratin antiserum (TK which detects 41–65 kd keratins), 2 monoclonal keratin antibodies (PKK1 specific for the 44, 46, 52 & 53 kd keratins and KL1, specific for the 55–57 kd keratins) and monoclonal antibodies for vimentin and desmin. The tumor epithelial cells were slightly positive or negative for PKK1 detectable keratins, but slightly to strongly positive for KL1 and TK antibodies. Tumor epithelium was slightly positive for vimentin but negative for desmin. The tumor foci were composed of both dark-staining and pale-staining cells; the former had a more intense reaction with KL1 and TK antibodies than the latter. Homogeneous acellular material was either PAS-positive or negative, with or without calcification, and keratin-negative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1988.tb01531.x
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  • 7
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of amylase in sialoadenitis and salivary gland tumors (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 15 (1986), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Immunohistochemical demonstration of α-amylase has been made in sialoadenitis-involved tissue and salivary gland tumors, as well as in normal salivary glands. Immunoreactive α-amylase with trypsin pretreatment was confined to irregularly staining serous acinar cells in the parotid and submandibular glands, and to demilunes in subligual glands. In obstructivce adenitis, staining was irregular from high to negative in acini in early or intermediate stages. Dilated ductal segments contained cells positive for α-amylase in the early stage following obstruction. Pleomorphic adenomas were usually negative for α-amylase but in rare cases tumor epithelia stained variably positive; i.e., staining occurred throughout the cytoplasm or at the periphery or apical part of the tumor cells. Luminal cavities of tubular and duct-like structures contained α-amylase-positive material. Epithelia in Warthin's tumor were also negative in general; however, scattered single or grouped tumor cells containing α-amylase were found. Mucoepidermoid tumors were also negative, though slightly positive cells were found intermingled among the negative squamous and mucous tumor cells. Cystic lesions in mucoepidermoid tumor were sometimes positive in the wall cells together with material secreted into the lumen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1986.tb00645.x
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  • 8
    Electronic Resource
    Electronic Resource
    Immunohistochemical observations on carbonic anhydrase I and II in human salivary glands and submandibular obstructive adenitis (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 15 (1986), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Immunohistochemical identification of carbonic anhydrase I and II (CA-I, CA-II) was made in human major salivary glands and obstructive adenitis in submandibular glands. Normal salivary glands stained the strongest for CA-II in serous acinar cells and were negative in mucous cells. Moderate to strong staining for CA-I and CA-II was found in ductal segments. Submandibular glands with obstructive adenitis exhibited reduced CA-1 activity in atrophic acinar cells, but not in ductal elements in the early and intermediate stages of the disorder. In the late stage of the obstructive lesion, CA staining in duct-like structures was moderate; however, almost degenerate ductal cells were negative for CA. During the progression of the degeneration in the obstructive lesion, the CA staining decreased dependent on acinar atrophy. Even after longstanding obstruction of the salivary gland, altered ductal epithelia may retain some of their functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1986.tb00604.x
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  • 9
    Electronic Resource
    Electronic Resource
    Studies on saliva in ODU plaque-susceptible rats having experimental gingivitis (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 20 (1985), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There was no statistical correlation between pocket probing depth of the mandible incisor and salivary IgA concentration in pilocarpine-stimulated whole saliva in ODU plaque-susceptible (Sus) rats which were susceptible to plaque formation on the lower incisor region and exhibited gingivitis. However, there was a highly significant negative correlation between the pocket probing depth and the amount of pilocarpine-stimulated saliva (r=−0.728, p〈0.01). These findings suggest that the amount of saliva may play a role in the pathogenesis of periodontal disease while salivary IgA is likely to be of less significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1985.tb00440.x
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  • 10
    Electronic Resource
    Electronic Resource
    Histochemical demonstration of lectinbinding sites and keratin in inflamed human gingiva (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 20 (1985), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Histochemical demonstration of lectin-binding sites and keratin peptides in gingival epithelia was reported and differences in staining and distribution were compared to inner and outer gingival epithelia.Gingival epithelia on the outer side exhibited zonal or regional distributions of lectin-binding, and the cytochemical staining was generally found in the cell coat and intercellular materials. Keratin protein was found frequently in the spinous cell, infrequently in the basal cells, and not at all in the superficial layer. The sugar residues in the cell coat of gingival epithelia probably were mannose, galactose, N-acetyl-galactosamine, and N-acetyl-glucosamine.The inner side of the epithelia, crevicular, and pocket epithelia were characterized by irregular and incomplete staining for lectins. These epithelia also displayed less keratin staining compared to the outer gingival epithelia. These findings suggest the possibility of depolymerization of glycosaminoglycans in the cell coat by enzymes of either pocket bacterial origin or host tissue origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1985.tb00847.x
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