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  • 1960-1964  (84)
  • 1920-1924
  • 1963  (43)
  • 1960  (41)
  • Biochemistry and Biotechnology  (80)
  • Synthetic methods  (4)
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  • 1960-1964  (84)
  • 1920-1924
Year
  • 1
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 309-330 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Biological purification of water pollutants depends upon a complex balance of organisms and environment conducive to effective metabolism. The bench scale test is useful to determine effects of a given waste water on that balance and to suggest controls promoting optimum purification. Applicability of laboratory results depends, in part, upon an experimental design similating anticipated operating conditions. This paper discusses advantages and limitations of historical bench-tests as a guide for design of tests giving data that are more useful during full-scale operation.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 50-gal. growth chamber is described together with its air filter and humidifier. The unit was designed especially for the production of Neurospora crassa, and was found to be effective in producing bulk amounts of mycelia with a high specific activity of tryptophan synthetase.
    Additional Material: 3 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 87-89 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Addition of the S-2-hydroxyethyl-, S-2-chloroethyl-, S-n-propyl-, and S-iso-propyl-derivatives of homocysteine, and methoxinine to S. aureofaciens (ATCC 13900) fermentations resulted in production of 7-chloro-6-demethyltetracycline in addition to the normally synthesized 7-chlortetracycline.
    Additional Material: 1 Tab.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 123-129 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of inoculating nutrient surfaces uniformly with bacterial cells has been developed. The method is based on controlled exposure of samples in a humidified chamber to a spray produced from a bacterial suspension. Factors affecting uniformity of inoculation (ionic strength of suspension, force of spray, distance between spray nozzle and sample, and exposure time) were studied in detail. Under optimum condition using agar medium in Petri plates, differences between total counts for six plates inoculated simultaneously, or between counts for individual 2 cm.2 areas taken at any location on any of the plates, were not statistically significant at the 5% level. The coefficient of variability for counts on 2 cm.2 areas renged from 8 to 12% between tests of 216 counts each.
    Additional Material: 3 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 185-192 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: By growing Aspergillus parasiticus (C.M.I. 15957) under controlled conditions on sterilized peanuts an average of 265 mg. of aflatoxin per kilogram of peanuts has been produced and subsequently isolated. Other strains from the A. flavus-oryzae group gave lower yields of aflatoxin and one such strain gave aflatoxin from which certain normal components were absent. The aflatoxin produced on sterilized peanuts by any particular strain of A. flavus-oryzae was shown by thin-layer chromatography to contain the same major components as were produced by that strain on unsterilized whole peanuts.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 221-230 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process has been developed giving over-all yields of about 50%: which could be increased to about 80% with higher material costs and processing time. The preferred method of recovering nucleotides from the cytoplasm has been thermal extraction by means of a heat exchanger followed by filtration, protein precipitation, and carbon adsorption. The concentrated eluate from the carbon is absorbed onto Dowex 1 ion-exchange resin, and groups of nucleotides are removed by pH-defined eluents and recovered by a second carbon adsorption. Finally, standard ion-exchange resolutions have been used to recover individual nucleotides. Other biochemicals in the extract have been investigated, and preliminary work has been done on the combination of nucleotide extraction with the recovery of nucleic acids from the residual cells.
    Additional Material: 3 Tab.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 231-242 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the present paper the authors described laboratory fermentors used in the Antibiotics Research Institute since 1958 for research concerning the technology of the biosynthesis of antibiotics and amino acids and other aerobic fermentations. The lids of the fermentors are made from stainless steel, the vessels are made from glass. The fermentors are placed, always four each, in eight water baths with automatic temperature regulation within a range of ±0.5°C. Details regarding construction, auxiliary equipment, and working procedures used for the sterilization of fermentors and media, inoculation, injection of additives, and defoaming are given. The set-up for one-stage and multi-stage, batch and continuous cultivation are discussed.
    Additional Material: 5 Ill.
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  • 11
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 131-146 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A sensitive cup plate assay method for lysine on large plates using S. faecalis ATCC 6057 as the test organism is described in detail. Well-defined zones of dense growth were obtained in a rectilinear dose - response range from 5 to 3000 μg. of L-lysine/ml. Comparative assays on twelve different samples were carried out using the turbidimetric tube assay method with Leuc. mesenteroides ATCC 8042 and the cup plate method with S. faecalis ATCC 6057. Good agreement between the two assay methods was obtained for five samples. For the remaining seven samples the tube assay values were appreciably higher than those obtained by the plate method. This discrepancy is discussed. Pure lysine added to various sample extracts could be quantitatively recovered. The specificity of the lysine requirement of S. faecalis ATCC 6057 in the plate assay was also investigated. Under certain experimental conditions, a weak and variable response was obtained with D-lysine and the polyamines cadaverine, spermine, and spermidine. There was generally no difficulty in distinguishing the D-lysine and polyamine growth zones from the L-lysine growth zones. This growth effect of D-lysine and polyamines could be diminished and even completely abolished by increasing the DL-hydroxylysine hydrochloride level in the plate medium. The lysine peptides glycyl-L-lysine and L-lysylglycine gave excellent growth zones of the same appearance as those produced by L-lysine. The activity of these two peptides in cup tests were on a molar basis about 70% of that of L-lysine.
    Additional Material: 2 Ill.
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  • 13
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 9-15 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method by which a paramagnetic oxygen analyzer is used to determine oxygen consumption and carbon dioxide production during aerobic fermentation of Torulopsis utilis is described. The volume of carbon dioxide produced is not necessarily related to the volume of oxygen consumed by the microorganisms. For this reason, all carbon dioxide is removed from the effluent gas before it enters the analyzer when determining oxygen consumption. A method for calibrating the analyzer to correct for this removal is outlined. When determining carbon dioxide, effluent gas bypasses the carbon dioxide absorption tower before entering the analyzer. The carbon dioxide which it contains dilutes the oxygen remaining in the gas. The magnitude of this dilution can be used to calculate the percentage of carbon dioxide produced in terms of the air flow to the fermentor. Equipment required to alternately measure and record oxygen consumption and carbon dioxide production is described.
    Additional Material: 4 Ill.
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  • 14
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 21-25 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cobamide production by Butyribacterium rettgeri Barker and Haas (ATCC 10825) was studied on a laboratory scale. Yields of the order of 5 μg./ml. were obtained when this anaerobe was grown for 6 days at 37°C. in a medium containing glucose, cornsteep liquor, cobalt nitrate, calcium carbonate, and sufficient ammonium hydroxide to keep the pH between 6.0 and 7.2. All of the vitamin was found associated with the bacterial cells, and examination of extracts of these cells showed the presence of 5,6-dimethylbenzimidazolylcobamide coenzyme in large quantities. Supplementation of the fermentation medium with a number of benzimidazoles resulted in formation of traces of “new” cobamides with these bases in the nucleotide protion of the cobamide. Supplementation with a number of purines did not result in incorporation of these moieties in the cobamide portion of the molecule.
    Additional Material: 1 Ill.
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  • 15
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    Biotechnology and Bioengineering 5 (1963), S. 49-52 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An investigation was made of the stability of product limited continuous cultures of Saccharomyces carlsbergensis and Pseudomonas ovalis. No oscillations were found in pH or glucose concentration during the steady state, and no overshoot resulted from changing from one steady state to another.
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  • 16
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 41-48 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacillus subtilis NRRL B-2612, isolated at this laboratory, was used to produce polyglutamic acid from devitalized wheat gluten. Conditions for production of the polypeptide were investigated. When NRRL B-2612 was grown on wheat gluten in shaken flasks with phsophate buffer and salts at pH 6.5, yields of polymer reflected a 25-40% incorporation of glutamic acid into polyglutamic acid. Repeated alcoholic precipitation yielded peptides containing between 45 and 90% D-glutamic acid. Physical and chemical properties of a typical purified polyglutamic acid indicated that the product was a gamma-linked polypeptide composed of 75% D-glutamic acid and 25% L-glutamic acid with a molecular weight of approximately 200,000.
    Additional Material: 3 Ill.
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  • 17
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 109-121 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbiological production of beta-carotene previously investigated in shakenflask culture has been scaled-up to 20-liter fermentors containing an operating volume of 10-liters. Yields obtained in shaken flasks, approximately 100 mg./100 ml. have been reproduced with a fermentation time of 72 hr. Factors affecting the fermentation: inoculum, mash ingredients, and environmental conditions, were investigated. Typical data are presented. A preliminary cost analysis indicates a “cost to make” of $31.35/kg. of carotene contained in the dried fermentation solids.
    Additional Material: 12 Ill.
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  • 18
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 167-184 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Shock pressure waves with a frequency of 50/sec. and a maximal pressure of 60-70 kg./cm.2 greatly reduced the viability of E. coli. A similar effect was obtained also on staphylococci, enterococci, Salmonella Zanzibar, and coliphages T2. On the contrary no effect on spores of B. subtilis was found. The effect was practically independent of the maximal pressure within the range from 30-80 kg./cm.2, the highest pressure tested. The effect decreased with increasing cell density of the bacterial suspension. Bacteria surviving shock treatment died considerably more rapidly when stored at 4°C. than bacteria treated in the same way except for exposure to pressure waves. Morphological examinations with the aid of ordinary light microscopy as well as electron microscopy suggest that the effect was due to mechanical action on the bacterial cell walls.
    Additional Material: 10 Ill.
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  • 19
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 20
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 287-307 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sphaerotilus is usually present in biological waste treatment processes and is considered to be a nuisance organism because it may cause bulking of activated sludge. However, it may play an important role in the decomposition of putrescible organic matter in properly operated biological waste treatment processes. The research described herein is a study of the variety of types of organic compounds which may be decomposed by Sphaerotilus and the effect of some environmental factors upon the rate of decomposition. Although there are some types of compounds which cannot be decomposed by Sphaerotilus, it is shown that this organism is able to assimilate and oxidize a large variety of organic compounds and that it is tolerant of an extremely wide range of environmental conditions. The role of Sphaerotilus in biological waste treatment is discussed in the light of the data presented.
    Additional Material: 9 Ill.
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  • 21
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    Biotechnology and Bioengineering 5 (1963), S. 355-365 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It has been found that a bacterium (isolant C12B) grew poorly on 6 and 8 carbon alcohols, well on 10 and 12 carbon alcohols, and slowly but well on the 14 to 18 carbon compounds. Unexpectedly, resting cells oxidized the 6 and 8 carbon compounds most readily and cell-free extracts oxidized 6, 8, and 10 carbon alcohols. Extracts from cells adapted to sodium lauryl sulfate oxidized the 12 carbon alcohol as well, if small concentrations of substrate were used. The bacterium grew on benzene sulfonate and adapted resting cells oxidized that compound. Pure culture degradation studies revealed that isolant C12B used only 11% of tetrapropylene ABS supplied as a sole carbon source, whereas 66% of sodium lauryl sulfate was degraded. Intermediate quantities of kerosene-based alkyl benzene sulfonate and a tallow-derived detergent were utilized. Extracts of another bacterium, TEG-5, grown on tetraethylene glycol, contained enzymes which oxidized polyethylene glycols up to an average molecular weight of 600, but not any larger polymers. This oxidation was visualized by coupling to reduction of ferricyanide.
    Additional Material: 9 Ill.
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  • 22
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    Biotechnology and Bioengineering 5 (1963), S. 17-20 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The applicability of physicochemical adsorption laws to the sorption of methylene blue by dead Penicillium chrysogenum was studied. The specific area (area/mass) of the mold was measured.
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  • 23
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 147-165 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of factors was found to affect the cup plate assay for lysine with Streptococcus faecalis ATCC 6057. Hydroxylysine, aspartic acid, and Tween 80 were found to be among the essential components of the assay medium. Removal of any of these three components produced diffuse growth zones or otherwise unsatisfactory plates. The inclusion of asparagine and additional dipotassium phosphate to the medium was found to be advantageous but not an absolute requirement. It was important not to autoclave the hydroxylysine and the additional dipotassium phosphate together with the rest of the assay medium, but to add these components to the sterilized medium immediately before pouring the plates. With certain concentrations of hydroxylysine in the medium, the addition of L-glutamine was found to potentiate the effect of hydroxylysine and thereby improve the lysine plates. The effect of graded amounts of the above mentioned medium components and of the pH of the assay medium was also studied. Increasing the sterilization time of the medium from 5 to 10 min. at 120°C. improved the readability of the lysine plates and led to more easily reproducible results. Factors influencing the preparation of the inoculum for the lysine plates were also studied: the age of the stab culture, the amount of inoculum used per plate, and the influence of the nature of the suspension medium used for washing the inoculum.
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  • 24
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 193-199 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ferrocyanide-treated beet molasses was fermented in fully baffled mechanically agitated, 2.5-liter, tank-type fermenters using pellets of Aspergillus niger grown in shake flasks as an inoculum. The object of the study was to adapt the fermentation method previously developed in an unstirred tower-type fermenter to a conventional tank-type fermenter. Under selected conditions of stirring (agitator speed 400-700 rpm) and sparging (medium porosity sintered disk), high yields of citric acid (8.6% citric acid, 70% conversion of available sugar) were obtained in 140 hr. of fermentation. There was little relation between citric acid yield and aeration conditions in the fermenter as judged by mash viscosity measurements and sulfite oxidation studies.
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  • 25
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    Biotechnology and Bioengineering 5 (1963), S. 53-58 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design of a cabinet for the aseptic manipulation of material is described. The interior surfaces of the cabinet are sterilized by swabbing with a germicide. Germ-free conditions are maintained during operation by means of a positive pressure of sterile air. A fan and filter unit attached to the cabinet is used to blow sterile air into the cabinet in sufficient quantity so as to flow out of the hand ports at a velocity of 50 ft./min. In the design, careful consideration was given to the ease of manipulations in the cabinet.
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  • 26
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Removal of the pungent factor, allyl isothiocyanate, will partly determine whether mustard seed can become a commerical source for oil and meal in the United States. In processing studies at the Northern Laboratory, the mustard glucoside was converted enzymatically and the pungent oil was removed. This process has now been extended to pilot-plant scale by using filtration-extraction equipment at the Southern Laboratory. After desolventization and further steam stripping, the extracted meal had a residual content of 2.9% crude fat and 0.004% allyl isothiocyanate.
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  • 27
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    Biotechnology and Bioengineering 5 (1963), S. 59-74 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The distribution of dissolved oxygen in agitated, sparger-aerated aqueous solutions has been investigated by polarographic techniques. The results of the investigations are discussed in relation to earlier studies in bubble distribution in such systems. Liquid-phase mixing is good at agitation intensities well below that necessary to disperse bubbles throughout the liquid.Suspended solids interfere with the transfer of oxygen unless they are freely suspended. Discrete fibers (paper-pulp) are much easier to suspend than molds in either filamentous or pellet forms. Mass-transfer at the cell-liquid interface appears to be unaffected, in both bacterial and mold suspensions, by the intensity of agitation, provided that the organisms are freely suspended.
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  • 28
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    Biotechnology and Bioengineering 5 (1963), S. 83-85 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The inclusion of ethionine in Streptomyces rimosus fermentation resulted in the production of N-methylethyloxytetracycline. The compound has been isolated, crystallized, and a number of its chemical properties determined.
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  • 29
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    Biotechnology and Bioengineering 5 (1963), S. 101-107 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An adsorption colorimetric method is proposed for the determination of the percentage of dead cells in suspensions of Penicillium chrysogenum, based on the observed fact that a linear relation exists between that percentage and the dye concentration at the equilibrium point.
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  • 30
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    Biotechnology and Bioengineering 5 (1963), S. 91-99 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbial polysaccharides which have many potential industrial applications are receiving more and more attention. One of the economic obstacles to the commercialization of these polysaccharides has been the cost of isolating them from fermented broth. To reduce this cost a recycling process was developed. The polyanionic polysaccharide synthesized from glucose by the activity of the bacterium Xanthomonas campestris NRRL B-1459 is precipitated from the fermented broth with a quaternary ammonium compound (QAC).QAC is removed from the precipitate with methanol leaving the water-soluble polysaccharide. Evaporation of the methanol wash removed the methanol and substantially all the QAC was recovered in the concentrate. This was recycled for subsequent precipitations. Chemical assay and the viscosity of water solutions of the isolated polysaccharide indicate no adverse effects on it from recycled QAC. The cost to make this polysaccharide by the described procedure is estimated to be $1.14/lb. This cost estimate includes land, buildings, raw materials, equipment, labor and supervision, utilities, factory supplies, working capital, and plant overhead.
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  • 31
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    Biotechnology and Bioengineering 5 (1963), S. 211-220 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A disposable plastic tetrahedron bag is described which is suitable for holding a 50 ml. culture on a shaker table. Bags can be made out of polypropylene or Teflon and they are quite simple and inexpensive. The tetrahedrons make excellent use of the shaker table space and they give a threefold increase in aeration efficiency over Erlenmeyer flasks.
    Additional Material: 4 Ill.
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  • 32
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 243-251 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The metabolic pathway of degradation of naphthalene to salicylic acid has been studied by several investigators. The rates of conversion and of cell growth are the basis for the present study. In the early phase of the fermentation, the salicylic acid accumulation followed an exponential curve, as did the bacterial cell accumulation. In the second phase, most of the salicylic acid was produced according to a linear or zero reaction, at rates of 0.3 to 0.5 g./l./hr. A linear accumulation of microbial cell weight accompanied the linear salicylic acid formation. Despite the correlation of growth and salicylic acid production, the type or amount of nitrogen supplied for growth had only a small effect on yield over broad limits. The optimum pH for salicylic acid accumulation was approximately 6.5. Below this value a slower rate of production and lower final yield was seen. Above pH 6.5, a rapid degradation of salicylic acid was observed. A temperature range from 25 to 30°C. was optimum. At 35°C., no growth occurred, while at 20°C. very slow salicylic acid accumulation resulted.
    Additional Material: 9 Ill.
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  • 33
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 255-273 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Stabilization ponds have been widely accepted in recent years, following studies that established deeper understanding of the phenomena associated with the stabilization process. A compilation of information on the use of both sewage and industrial ponds is presented showing types and geographical location in the United States. A total of 1,304 stabilization ponds currently are used as the principal method of sewage treatment serving a population of 2,138,085. Ponds are used for tertiary treatment serving a population of 2,146,951, in 343 instances. Use of 827 industrial ponds by 31 industries in 44 States is also indicated. Indiana ranks first in usage with 159 ponds and Callfornia second with 140. Ponds were classed as effective by 66 percent of the 455 users who reported on this item. Of 149 reports on odors, 73% indicated nuisances. Insect problems were reported in only a small number of instances. Data are presented on design considerations and the mechanism of waste stabilization ponds. Aerobic ponds are given greatest emphasis. Photosynthesis and its dependence upon the algal mass, suitable temperature, incident light penetration, nutrient supply, and induced vertical mixing by wind are of prime importance in the stabilization mechanism. Odors are associated with prolonged anaerobic conditions, and these may persist up to 4 weeks following extended ice cover in cold climates, if BOD loadings are 25 lbs. per acre per day or greater. Nitrogen and carbon may be limiting factors in the development of an algal mass. Coliform, BOD, and nutrient removals are presented for various physical, chemical, and climatic conditions. Although fecal streptococci removal averaged 97% in a study pond, they were still discharged in the effluent at a rate of 700,000 per second in winter and 70,000 per second in summer. A striking similarity exists generally among the algal speciation in stabilization ponds, regardless of geographic location. The algal mass is, however, dependent upon unique pond conditions and location, and may vary upwards to nearly 5 million algal cells per milliliter, 34,000 p.p.m. by volume, or 30-35 tons per acre per year. The problem of blue-green algal mats and resultant pigpen odors in extremely localized areas is duscussed. Midges and midge larvae may have an impact on the nutrient cycle in a stabilization pond, and may pose a potential localized nuisance in some areas. Mosquito problems have usually been associated with growth of aquatic vegetation. Suggestions for minimizing the potential problem are discussed.
    Additional Material: 3 Ill.
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  • 34
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 201-209 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is a well-known fact that choosing a sequence of optimal control variables in each reactor tank of a multistage reactor system can result in a considerable increase of the desired product for a consecutive reaction system. The discrete maximum principle is applied to a generalized optimization problem of first order consecutive biochemical reaction system. A detailed numerical solution as an example is also presented for illustration.
    Additional Material: 1 Ill.
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  • 35
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 275-285 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Phenol-bearing waste waters continue to be an area of wide concern for various industries faced with the task of cleaning up effluents containing relatively small amounts of these materials. The microbiological aspects of the degradation of these materials in pure culture and waste treatment plants is reviewed. Actual operating experiences at three plants of The Dow Chemical Company, where phenols are present in combination with other widely varying waste constituents, are discussed.
    Additional Material: 6 Ill.
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  • 36
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 331-345 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: These experiments show that ABS can be adsorbed and desorbed from microbial cell surfaces. The adsorption equilibria between the free and adsorbed phases are primarily influenced by the pH of the system. The effects of ABS upon the biochemical activities of bacteria depended upon the amount of ABS adsorbed and the type of bacteria present. The behaviour of ABS is essentially the same whether in the presence of pure or mixed bacterial cultures. Two desorption experiments with soil from a reclaimed water spreading basin which has received sewage over a six month period showed that at least 82% of the ABS “loss” was recoverable from the soil. In all the experiments carried out in this study no evidence was obtained for the biochemical degradation of ABS.
    Additional Material: 15 Ill.
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  • 37
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 347-354 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the emphasis being given to the development of biologically soft detergents, it is necessary to establish eriteria defining biodegradability of these materials. It is the authors' opinion that a biologically soft detergent should have the following characteristics: (1) Under normal operations the material should be 85% removable by activated sludge. (2) Normally high discharges of the detergent should not influence biological activity. (3) The detergent should ultimately degrade to carbon dioxide and water.
    Additional Material: 6 Ill.
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  • 38
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 367-384 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Because branched-chain alkyl benzene sulfonate (ABS) is well known to create water pollution problems in surface water and ground water, a study was made on the biodegradation of this surfactant along with other anionic and nonionic surfactants in detergents by water bacteria. Biodegradation was evaluated by the conventional river die-away test employing the methylene-blue method or surface-tension techniques as analytical tools. Under anaerobic conditions, sugar detergents (sucrose esters) seem to be an ultimate solution to water problems created by branched-chain ABS; in aerated water, straight-chain ABS, sulfo tallow methyl esters, and nonionic surfactants are preferable to branched-chain ABS to improve water problems.
    Additional Material: 10 Ill.
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  • 39
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 1-7 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A cost analysis of the continuous fermentation of penicillin G shows that materials cost and product yields are more significant than throughput rate. Number of fermentation stages, productivity, and the implications for research and development are discussed.
    Additional Material: 2 Ill.
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  • 40
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    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 2 (1963), S. 24-29 
    ISSN: 0570-0833
    Keywords: 3-Aminoquinazol-4-one ; 3-Aminobenzotriazin-4-one ; Quinazolones ; Benzotriazinones ; Synthetic methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Among the derivatives of 3-aminoquinazol-4-one and 3-aminobenzotriazin-4-one are compounds with narcotic, sedative, analgetic, and muscle-relaxant properties. Of special interest are compounds with one or several urethane groups and those substituted in the 3-position by a morpholine moiety.
    Additional Material: 4 Tab.
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  • 41
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    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 2 (1963), S. 225-238 
    ISSN: 0570-0833
    Keywords: Pyridinium salts ; Synthetic methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This review discusses syntheses leading to a new type of N-vinylpyridinium salts, to novel anionocyanines, to aminophenazines and alloxazines, as well as to (aza-) dehydroquinolizinium, acridizinium, and morphanthridizinium salts. In addition, Mannich reactions and aminoalkylations at active methylene groups of cycloimmonium salts, and their reactions with amines are treated. The possibilities offered by the “Ortoleva-King” reaction are demonstrated using numerous examples, such as a new route leading to the yohimbine skeleton.
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  • 42
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    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 2 (1963), S. 309-323 
    ISSN: 0570-0833
    Keywords: s-Triazines ; Synthetic methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The preparation and properties of s-triazine are discussed. Nucleophilic reagents (even water) readily attack s-triazine. Thus, open-chain and heterocyclic compounds, e.g. formamidines, aminomethylene compounds, and triazole, pyridine, and pyrimidine deravatives are accessible from s-triazine. With amidines and imido ethers substituted triazines are obtained (by trans-triazination).The latter are formed via open-chain intermediates.
    Additional Material: 11 Tab.
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  • 43
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    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 2 (1963), S. 380-393 
    ISSN: 0570-0833
    Keywords: Pyridinium salts ; Synthetic methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Cleavage of N-phenylpyridinium or N-vinylpyridinium salts with secondary aliphatic amines leads to aromatic amino compounds or enamines which are often unobtainable by other routes. The other fragment, glutacondialdehyde or its monoanil, gives azulenes, as shown by Ziegler and Hafner, as well as König; these compounds can also be prepared from alkylpyridinium salts with cyclopentadienylsodium (Hafner). Syntheses of labile aldehydes which are otherwise difficult to obtain and of α-ketocarboxylic acids, starting from pyridinium salts and proceeding via nitrones or α-cyanoanils, are illustrated by many new examples. Benzimidazoles, benzothiazoles, and amidines are accessible from pyridinium salts via nitrones or α-cyanoanils; cyanoanils can be transformed into quinoxalines (including xanthopterine), perimidines, etc. Furthermore, nitrosonaphthols and nitrosonaphthylamines react with pyridinium salts containing an active methylene group on the nitrogen, leading to new ring systems. Further synthetic possibilities arise from nucleophilic displacements of the pyridine in pyridinium salts by SN 2 reactions. Combination of the route via nitrones with the methods of Arndt-Eistert, Ortoleva-King, or with chloromethylation constitutes a significant enlargement of its range of application. The formation of α-cyanoanils from diazoketones permits synthesis of α-keto-β-amino acids from α-amino acids, or of phenylglyoxylic acids from benzoic acids by chain extension.
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  • 44
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dextran, a polymer of glucose, has been widely used as a blood-plasma volume expander. In whole culture fermentations most dextran-producing organisms elaborate dextrans that have molecular weights of many millions and must be depolymerized and fractionated to obtain a product suitable for clinical use. An exception however is Streptococcus sp. (Strain DS-50) NRRL B-1351. In the work described here, this organism is used to produce clinical-size dextran in high yields, both on a laboratory- and a pilot-plant scale. With this process, fractionation is required but depolymerization is not. Fermentations of media containing 15 per cent sucrose are complete in less than 48 hours with 0·2 per cent inoculum. Yields of native dextran and clinical-size dextran by methanol precipitation are 68 and 43 per cent of the theory, respectively. The structurally heterogeneous dextran formed by NRRL B-1351 has a higher viscosity for a given molecular weight than does NRRL B-512 dextran. The viscosity-molecular weight relationship for the former may be expressed by the equation [η] = 6·51 × 10-4Mw0·554. The high-molecular weight fraction had approximately 75 per cent of 1,6-like linkages, as compared with 87 per cent for the clinical fraction. All fractions had a lower ratio of 1,6 to non-1,6 linkages than did NRRL B-512 dextrans.
    Additional Material: 6 Ill.
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  • 45
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 447-451 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hot soaking of paddy in the parboiling process does not significantly affect the digestibility of rice compared to ‘raw’ rice. Mechanically dried parboiled rice appears to be slightly more digestible in vitro than sun-dried parboiled rice. It is also significantly superior in thiamine value.
    Additional Material: 3 Tab.
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  • 46
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 481-500 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Control of product quality throughout repeated fermentations depends upon maintenance of genetic uniformity from the time of strain selection until the product is harvested. Genetic control, therefore, must be maintained for stock cultures, and during inoculum preparation and growth to produce the product. Theoretical and applied aspects of a procedure that will accomplish this control are discussed. A specific procedure adaptable to situations in which a ‘seed’ is repeatedly increased to produce a larger quantity of a final material is described. The procedure was tested by comparing the product of three bacterial species grown by a standard or usual vs. recommended or experimental procedure comparing product quality and uniformity. Use of the recommended procedure resulted in the high production of a uniform product based on the maintenance of the colony type of Bacillus anthracis, colony colour and type of Serratia marcescens, and uniformity and amount of toxin produced by Clostridium botulinum type A.
    Additional Material: 6 Ill.
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  • 47
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 31-48 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Production of oligomycin has been studied in fermentations ranging from 100 ml in shaken flasks to 1000 gal in agitated and aerated tanks. The yields ranged from nothing to 3675 μ;g per ml. The main factor favouring the production of oligomycin was a medium giving good growth and supplying an abundance of slowly utilized energy. Media meeting these requirements contained yeast extract and/or molasses for growth and lard oil for energy. Glycerol, glucose or starch as the main source of carbon gave poor results. A pH close to 7 favoured oligomycin production. Considerable organic nitrogen was synthesized and excreted by the mycelium during oligomycin production.Under suitable conditions practically all of the oligomycin remained in the mycelium. Extraction of the mycelium with acetone, concentration of the extract, removal of oily material with a low-boiling hydrocarbon, decolourization and final crystallization from methanol or ethanol were the main steps in the recovery process. From pilot plant and tank fermentations about 4500 g of crystalline oligomycin have been prepared.Three oligomycins, A, B and C were found by paper chromatography in even well-crystallized oligomycin. In different lots the proportions varied from 10-75 per cent A, 4-90 per cent B and 0-36 per cent C. High yields and high percentages of A and C were obtained on the neutral, lard-oil medium. Low yields and high proportions of B were given by the glycerol, low-pH medium.
    Additional Material: 1 Ill.
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  • 48
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pilot plant was built to evaluate the economic potentialities of producing glycerol from sugar by the bisulphite fermentation process. The design of this small unit was based on information collected from bench-scale studies of the fermentation and recovery processes taking advantage of new separation techniques.A beer containing 5 per cent of glycerol is produced in a continuous fermentation in which the free bisulphite level is automatically controlled. The effluent from the fermentor is centrifuged, and the yeast is returned to the fermentation. The dilute clarified beer is acidified; sulphur dioxide, acetaldehyde, and alcohol are removed; and the stripped solution is neutralized and concentrated until it contains 45 per cent of glycerol. This concentrated glycerol stock is purified by using a combination of ion-exclusion and ion-exchange.Good fermentation control results in a beer that has a low solids-to-glycerol ratio, low organic acid content, and low residual sugar. The method of glycerol purification results in a high glycerol recovery, since no distillation of glycerol is involved. The sulphur dioxide removed from the beer is recycled through the fermentation so that the chief raw materials used in the process are sugar, sulphuric acid, and sodium carbonate. The yields of products based on sugar charged are: glycerol, 25 per cent; alcohol, 17 per cent; and acetaldehyde, 11 per cent.The economic success of the process depends to a large extent upon the price of sugar. Under present conditions it is felt that the process is competitive.
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  • 49
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 49-70 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new vibrator, commercially available as the Sonomec Wave-Pulse Generator, is described, in which micro-organisms may be disrupted by shaking with glass beads. The amplitude and frequency of vibration of the shaker may be varied independently by suitable linkages incorporated in the mechanism. A study has been made of the effect of several variables, particularly the height of the liquid column in the vibrating container, on the rate of disruption. A relation is shown to exist between the acoustic heating produced in the container by the passage of sound waves through the liquid and the rate of disruption of baker's yeast. It is considered that at most depths of the liquid column, the action is similar to that of other shakers, but at a certain depth of liquid, additional forces arise which greatly increase the rapidity of disruption. How these forces act is not known. Factors affecting the rate of disruption of baker's yeast, Corynebacterium xerosis and Bacillus megatherium have been studied and a comparison has been made of the fractions obtained by differential centrifugation of the cell dispersion and compared with similar preparations from the Hughes press.
    Additional Material: 6 Ill.
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  • 50
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 93-112 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Filtration theory is briefly reviewed with regard to possible application to sterilization by filtration. Of the different devices tested, Seitz asbestos sheets, acid-treated to remove toxic materials, offered most advantages for routine use and scale-up potential. The standard law of Hermans and Bredée best expressed mathematically, the filtration of tissue culture media sterilized by Seitz No. 6 laboratory filters. Data are presented showing the correlation between the performance of the Seitz No. 6 filter, and the Seitz No. 200 plate and frame press (involving a scale-up ratio of up to 400 to 1). An expression: \documentclass{article}\pagestyle{empty}\begin{document}$${{t = 3 \cdot 25 \Delta P^{ - 0 \cdot 627}} \mathord{\left/ {\vphantom {{t = 3 \cdot 25\Delta P^{ - 0 \cdot 627} } {{{(A} \mathord{\left/ {\vphantom {{(A} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}} \right. \kern-\nulldelimiterspace} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}}}} \right. \kern-\nulldelimiterspace} {{{(A} \mathord{\left/ {\vphantom {{(A} {V_f + 0 \cdot 001{\rm }\Delta P - 0 \cdot 036)}}} \right. \kern-\nulldelimiterspace} {V_f + 0 \cdot 001 \Delta P - 0 \cdot 036)}}}} $$\end{document} derived from experimental data obtained, expressing time in terms of pressure, surface area, and volume of filtrate, enabled prediction of filtration parameters with accuracy acceptable for engineering purposes for unclarified media, and greatly improved for media which had been initially clarified.The influence of streaming potential is discussed with regard to its influence on the course of a filtration.
    Additional Material: 7 Ill.
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  • 51
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 71-80 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ambler's Q/Σ relationship between the throughput of a centrifuge and its mechanical characteristics has been translated from batch data in order to predict operating conditions for continuous centrifugation in virus processing. A Sharples Laboratory Supercentrifuge was converted from batch to continuous operation and a slurry feed device was developed. A correlation between operating conditions for continuous centrifugation and amount of test virus recovered from the feed showed that the Q/Σ values at which high concentrations of the virus were recovered were almost identical to the Q/Σ values for significant virus recovery in batch centrifugation. Titration and gravimetric determinations of the sediments recovered from continuous centrifugation operations indicate that product purity might be influenced by operating conditions.
    Additional Material: 4 Ill.
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  • 52
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 53
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 121-127 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Equipment has been developed for resuspending or redissolving protein precipitates separated in a continuous-flow centrifuge without first removing them from the centrifuge bowl. All the components can be sterilised by autoclaving. The equipment has been used successfully in selected stages of a process for the preparation of human plasma fractions.
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  • 54
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oxygen transfer has been studied in the Pseudomonas ovalis fermentation that quantitatively converts glucose into gluconic acid. The rate of oxygen uptake by this system was correlated with aeration rates and agitator speeds over wide ranges of these variables, by the introduction of a liquid expansion term. The technique described provides a biological method for studying oxygen transfer in the P. ovalis system; its general principles can be applied to other fermentations.
    Additional Material: 5 Ill.
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  • 55
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 25-29 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrogenase system of Hydrogenomonas facilis could be induced by either hydrogen or deuterium and the rate of deuterium uptake by the induced cells was the same as that of hydrogen.An attempt has been made to detect discriminate use of hydrogen and deuterium by bacteria. No discrimination could be detected during hydrogenation of methylene blue by Escherichia coli, under the experimental conditions.
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  • 56
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 81-91 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Development of a foam breaker which operates on the principle of rapid acceleration of foam through a nozzle has allowed large-scale aerobic fermentations to be run without addition of antifoam agents. Fermentations have been investigated over a wide range of aeration levels in which oxygen supplied to the organisms was the limiting growth factor.With Torulopsis utilis grown in a 5 per cent molasses medium. oxygen utilization followed an exponential pattern during the early phase of growth, with carbon dioxide production equal to the consumption of oxygen. At the point where oxygen supply to the organism became limiting, anaerobic fermentation began, and the consumption of oxygen remained constant. The level of oxygen absorption beyond which the respiratory quotient exceeded unity was used as a measure of limiting oxygen supply.Addition of three types of antifoam compounds to T. utilis fermentations resulted in a marked depression of oxygen consumption, and a decrease in cell production. Incremental addition of small quantities of antifoam to maintain a constant foam level in the fermentor depressed the oxygen absorption rate to a lesser extent. Sulphite oxidation rates did not reflect the true value of oxygen absorption rates found for fermentations without addition of antifoam.
    Additional Material: 6 Ill.
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  • 57
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 143-155 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Control of foams by oil additions is of large economic importance to the fermentation industry. Excessive foaming causes loss of material and contamination, while excessive oil additions may decrease the product formation. Antifoam oils may be synthetic, such as silicones or polyglycols, or natural, such as lard oil or soybean oil. Either will substantially change the physical structure of foam, principally by reducing surface elasticity.Industrial antifoam systems usually operate automatically from level-sensing devices. Methods for metering of oil under aseptic conditions are: timed delivery through a solenoid, two solenoids with an expansion chamber between, a motor-driven hypodermic syringe, and certain industrial pumps.Distribution devices can improve efficiency over straight-pipe entry. Types described are spray distribution, diverter bars, and wick devices.
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  • 58
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 165-175 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The broths of four important industrial fermentations, namely nystatin, penicillin, and streptomycin fermentations, and a fermentation used for steroid hydroxylation, were examined rheologically. Considerable variation in rheological properties was demonstrated among these fermentations as well as throughout the course of the nystatin and streptomycin fermentations. These latter fermentations, employing Streptomyces strains, were mostly Newtonian in behaviour. Departures from this behaviour twice in the streptomycin fermentation resulted in plastic characteristics. The other two fermentations employing moulds were found to be markedly non-Newtonian, either plastic or pseudo-plastic in their behaviour.Rheological properties of most fermentation broths change appreciably during the course of fermentation. These properties influence the nature of the fluid regime in fermentors and, as a consequence, are important factors when considering mass and heat transfer. Therefore, a knowledge of the rheological behaviour of fermentation broths is an important prerequisite for an intelligent understanding of these transport processes. This paper briefly reviews some of the mathematical flow models used to characterize fluids and reports the rheological examination and analysis of several industrially important fermentation broths containing filamentous micro-organisms.
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  • 59
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 177-186 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The present study was conducted to determine the feasibility of growing bacteria in quantity on solid media in closed vessels. Five techniques were investigated in the laboratory, including growth on agar, growth on packing in beds, growth on solids in a fluidized bed, growth in foamed medium, and growth on semipermeable membranes. Data obtained from the laboratory experiments were used to scale up these techniques on paper and compare the projected plants with a conventional submerged fermentation production. All the techniques compared unfavourably with submerged culture on the basis of plant and operating costs, operating complexity, equipment complexity, and safety. One technique of surface culture, growth on semipermeable membranes, appears to offer a decided advantage over submerged culture, in that the cell product can be recovered directly as a paste. Further work on this technique is recommended.
    Additional Material: 6 Tab.
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  • 60
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A composite of several studies by the authors is presented in an attempt to illustrate the use of the suspension culture method to analyze population dynamics of an animal cell strain. On the basis of these studies the nature of the lag and plateau phases is discussed. More extensive discussions of the various studies in relation to the work of others appear elsewhere.
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  • 61
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 299-311 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A system is described which uses photosynthesis to regenerate oxygen from exhaled carbon dioxide in sealed cabins in the absence of gravity. Design considerations as to choice of algal strain, illumination, nutrients and gas-exchange methods are discussed. Details are given of such an apparatus, of laboratory size, used to evaluate selected semipermeable gas-exchange membranes. Data is presented and extrapolated to estimate size and weight of a possible manned space system.
    Additional Material: 6 Ill.
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  • 62
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 313-325 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new strain of cells, designated as PD-MEK-I, and derived from the kidneys of a rhesus monkey embryo, has been isolated and utilized as a prototype for mass propagation in suspended cell cultures, using techniques similar to those developed for antiobiotic fermentations. The use of the large-volume units for cultivation of tissue cells has been applied. These units are useful for preparing masses of cells for studies of cellular antigens, virus assays, antitumour cytotoxicity studies, and other biologically active materials, as well as seed for continuing cultures. In addition, other methods of roller bottle cultures, shaker flask cultures, spinner cultures, and disposable 1-litre prescription bottles, have been modified so that over 35 different human and animal cell lines are being maintained in continuous culture. Any one of these procedures may be modified for preparing and handling large numbers of replicate cultures as well as for maintaining stock seed cultures. The characteristics of growth of cells utilizing these methods are similar to those encountered in microbial populations and the principles of antibiotic fermentation have been applied appropriately.
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  • 63
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 327-338 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pilot plant is described for the mass cultivation of foot-and-mouth disease virus, in vitro, on calf kidney cell monolayers. The pilot plant produces up to 400 litres of virus per week; it can be enlarged with ease to a sufficient capacity to meet any requirements.
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  • 64
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The submerged propagation of plant tissue and cell cultures has now reached the stage where mass amounts of material can be produced. In discussing these advances, various aspects of the technology involved are discussed: the methods of isolation and establishment of cultures, the media used for culture and their effects on growth, the systems used for growth and a comparison of their effectiveness.Biochemical differences are shown between cultures grown under in vitro conditions and the organ from which they were derived. These differences are found among amino acids, organic acids, nucleic acids, and sugars. The potential use of the tissue culture approach for the study of the biosynthesis of ‘secondary compounds’ such as alkaloids and steroids is discussed.
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  • 65
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 66
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 361-379 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Several strains of Pseudomonas were selected by the screening test for organisms which are capable of producing L-isoleucine.With these selected bacteria, various cultural conditions suitable for the production and accumulation of L-isoleucine were investigated. As a result, 12-14 mg/ml of L-isoleucine was formed by shake culture in a medium containing α-aminobutyric acid, glucose, cornsteep liquor, urea and inorganic salts.
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  • 67
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 339-359 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Engineering data on the design and operation of algal culture systems for photosynthetic gas exchange are virtually non-existent. The authors have conducted intermediate and definitive level engineering studies to characterize algal systems - with a view to extrapolating to larger-scale systems for life support in closed spaces.Light intensity, carbon dioxide concentration, and dilution rate were the principal parameters used to control the photosynthetic rate and, consequently, oxygen production. Carbon dioxide absorption rate, equilibrium density, and cellular growth rate were also investigated.It was found that: (1) properly jacketed high intensity, incandescent lamps provided a suitable light source for growing algae; and (2) physiologically safe (0·5 per cent) concentrations of carbon dioxide produced growth comparable to that obtained at higher concentrations.A dilution rate of nearly 0·1 volume change per hour produced the best oxygen yield (2·41 × 10-3 lb/h) for the definitive system. Maximum cell doubling time was 5·1 h. The highest culture density attained was 5·9 mm3/ml, and the maximum dry weight algae yield was 3·0 × 10-3 lb/h.
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  • 68
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 401-410 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Previous research has shown that a reduction in pH of digested sewage sludge considerably improves its filtration characteristics. Experiments with sulphur oxidizing bacteria (thiobacilli) have shown that under aerobic conditions with a supply of elemental sulphur the pH can be reduced by microbial sulphuric acid synthesis. A semi-continuous process of sludge conditioning by this method was evolved, which would continue if the pH did not rise above pH 7 and if sufficient sulphur was supplied. Attempts to substitute sodium thiosulphate, or sulphide-rich sludge, for elemental sulphur were unsuccessful. A note is appended on a method of enumerating the thiobacilli.
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  • 69
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 70
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 1-8 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Combined irradiation-heat processing is shown to be syner-gistic for killing anaerobic bacterial spores inoculated into canned green peas. Following 1·2 megarad of gamma irradiation an F0 of 0·5 sterilized green peas inoculated with either 5,000,000 Clostridium botulinum 213 B or 300 PA 3679 spores per can. This processing schedule must be considered a minimum since it is based on a limited number of cans. Because sterilization by either irradiation or heat alone can damage the organoleptic properties of canned foods, the lowered schedules possible with combined processing may prove to be of value for preserving vegetables as well as meats.
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  • 71
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 113-120 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is shown that at -25°C alterations in the crystal structure of ice caused by repeated pressure changes in the regions of 2000 and 3500 kg/cm2 are accompanied by a disruption of E. coli cells suspended in the ice.
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  • 72
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 157-163 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The utility and the practical precision of the Most Probable Number method for determining bacterial populations is currently restricted by a lack of adequate tables of solved examples or alternatively by an easy and quick solution to individual problems. Where an electronic computer is available, these restrictions need not apply. A computer programme and example solution for tabulated data and individual problems are presented in this paper.
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  • 73
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 215-225 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The sampling method of polarographic analysis for dissolved oxygen in culture medium is unaffected by the presence of viable micro-organisms. This method allows measurement of peak oxygen demand, critical oxygen concentration, and oxygen supply during actual mass propagation of an aerobic culture. Fermentation operating conditions may be adjusted to supply the oxygen required by the aerobic culture.
    Additional Material: 6 Ill.
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  • 74
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 227-241 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth of Saccharomyces rouxii, an osmophilic yeast that produces glycerol and arabitol, was investigated using chemically defined media in continuous culture. Total polyol production increased with increasing aeration and growth rate. At very low aeration levels ethanol was produced but as aeration levels were increased ethanol could not be detected, formation of d-arabitol remained constant and formation of glycerol increased.In fully aerobic cultures with a replacement time of twelve hours, nitrogen-limited growth resulted in accumulation of glycerol in the medium. In glucose-limited growth glycerol tended to decrease. It was probably metabolized as a secondary carbon source. The optimum temperature for polyol production was 30°C while that for cell growth was 27°C.
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  • 75
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 76
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 205-214 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oxygen tension in aqueous media of the order of 10-6 to 10-9 molar is measured amperometrically by means of a stationary inert metal electrode. The electrolytic cell is connected into a high resistance bridge circuit, which is energized from a low resistance d.c. source of voltage adjusted to give a potential difference across the cell corresponding to a position on the diffusion current plateau of the first oxygen wave. This cell potential is monitored by means of a vacuum tube millivoltmeter and maintained constant. A vacuum tube millivoltmeter measures the bridge output. The bridge is initially balanced with the medium deoxygenated; subsequent introduction of low concentrations of oxygen dissolved in the medium unbalance the bridge. Either the resulting bridge output voltage, as read on the millivoltmeter, or the resistance change in the bridge arm opposite the cell, is related to the oxygen tension by means of a previous calibration. Both quantities are directly proportional to oxygen concentration, at least over limited ranges of such concentration changes. The method is also applicable to the estimation of other substances which can be discharged under amperometric conditions at a stationary electrode.
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  • 77
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This study is designed to establish fundamental data which will permit the eventual application of algal culture to the removal of nutrients from sewage plant effluents. A test cell is described which gives reproducible data when environmental parameters are varied. The effect of changes in temperature, light intensity and culture density as reflected in the growth rate, are shown, and the influence of these parameters on the removal of nitrogen and phosphorus under the test conditions indicated. The importance of an adequate carbon source and proper pH control is demonstrated. The organisms used were Scenedesmus and Chlorella in mixed or unialgal cultures.
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  • 78
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 243-252 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The process for the production of mushroom mycelium for food purposes has great potentiality. It would offer a simple, mechanized, inexpensive method for producing a nutritious food. Mushroom mycelium can be cultivated in submerged liquid culture on simple carbohydrate and nitrogen compounds with mineral salts. Yields are high and no special production problems are involved. Those species reported to have flavour are Agaricus campestris, Morchella crassipes, Lepiota rachodes, and Coprinus comatus. The mycelial growth is usually either in ball form or dispersed. The dispersed form, characterized by fine mycelium and secondary spores, may be a physiological mutation which gives greater yield but less flavour. Mycelium grown on solid media is said to have greater flavour than mycelium grown in submerged culture. Several methods for enhancing the flavour of the mycelium have been investigated. The taste of the mycelium has been variously reported from flavourless to equivalent or even preferable to that of the fruiting bodies. The fact remains, however, that it is only the problem of taste that has kept the process from commercial exploitation.
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  • 79
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 267-285 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fourteen mammalian cell strains were studied in the ‘stirrer culture’. Fair to excellent growth was obtained with twelve of these strains while two strains failed to show evidence of proliferation. Growth curves, maximal population densities, and recovery following subculture of various cell strains, cultivated in this system, were described. Factors effecting the growth of cells under these conditions were outlined. Growth of cells in methods other than the ‘stirrer culture’ were reported. The practical importance of fluid suspension culture of mammalian cells, the potentialities and the problems involved in scaling such cultures to industrial size were discussed.
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  • 80
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 411-417 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A rapid method for the assay of Δ1,4-androstadiene-3, 17-dione has been worked out for use in the control of its production by biological oxidation. The method consists of a single selective extraction step, a specific colour reaction and a photometric measurement. Based upon the analyses of samples taken at frequent intervals, the oxidative process can be stopped at its optimum production level. Thus insufficient transformation and over-oxidation can both be avoided. The time needed for one analysis is 20 minutes.
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  • 81
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 439-445 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A compact dryer for the parboiling of paddy has been described. The use of steam tubes along the circumference of the shell has made high drying rates possible. The rate of water removal is dependent on steam pressure in the tubes. Blowing of pre-heated air through the dryer shell is necessary. The optimum drying conditions for parboiled paddy as observed in the experimental rotary dryer are: (a) the steam pressure in the tubes should be about 45 lb/in2 gauge; (b) the feed rate should be 150 lb/h, approximately; (c) the rotation should be about 5 rev/min; (d) the inclination of the shell should be nearly 2° to the horizontal and (e) air at a rate of 100 ft3/min should be blown parallel to the feed, pre-heated to a temperature of 90°C.
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  • 82
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 431-438 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The conventional parboiling process used in the Orient consists of steeping paddy in cold or lukewarm water for 48-72 hours, steaming for 15-25 min and then drying in the sun. This process yields rice of a poor quality, and an attempt has been made to improve it by devising both batch and continuous processes in which the soaking and steaming are carried out in the same equipment. The processes developed give a larger throughput and also a product of a quality superior in appearance, flavour and thiamine content to that produced by the usual parboiling method.
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  • 83
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 419-430 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The use of low-level radiotracer techniques permits rapid, precise estimation of yields and material balances for processing steps. This is a great advantage for development work with Vitamin B12 because the conventional methods using microbiological assays are slow and lack precision. Release of the vitamin from S. griseus cells, selection of absorbing resins, and screening of eluting agents were followed using cobalt-60 cyanocobalamin. The method is most valuable when supplemented by microbiological assays because the yields of radioactivity are misleading in conditions which inactivate the vitamin.
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  • 84
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    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 453-479 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new press (the X-press) has been developed for the disintegration of cells in the frozen state (mostly at -25°C). By forcing the frozen cells contained in a cylinder through a hole much smaller than the diameter of the cylinder but much larger than the size of the cells, a satisfactory disintegration can be obtained.By forcing the cells through the hole repeatedly rather than once, a higher degree of disintegration can be achieved. By making the receiver identical to the charge chamber, repeated operations are possible without uncoupling the press.The disintegrated material is very easily recovered - it is simply taken out of the press in the form of a cylinder containing the frozen, disintegrated material which is then easily homogenized.This principle has been found satisfactory for the disintegration of a large number of cells, viz. bacteria, yeasts, moulds, plant and animal cells and, with regard to biochemical questions, it has been found to be superior to existing methods which do not treat the bacteria in the frozen state. The mechanism of the disintegrating action is discussed.
    Additional Material: 12 Ill.
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