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  • 1970-1974  (440)
  • 1930-1934
  • 1970  (440)
  • Life and Medical Sciences  (413)
  • Electron microscopy
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 132 (1970), S. 107-157 
    ISSN: 1432-0568
    Keywords: Differentiation ; Down feathers ; Electron microscopy ; Keratinization ; Keratohyalin granules ; Periderm granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The morphology of the developing chick feather germ (down feather) was studied at the ultrastructural level from 8 to 18 days of incubation. The process of keratinization in the developing feather germ was described, discussed and compared to keratinization in mammalian skin and hair. This study has shown that: 1. Apico-basal gradients of differentiation and different cell types are recognizable at the ultrastructural level in the developing feather germ. 2. The hypothesis that keratin is synthesized de novo by ribosomes is probably correct, because the largest number of these organelles is present at the time when keratin formation is most prominent. 3. Intercellular gaps in the developing feather germs facilitate the reorientation and rearrangement of different cell types into definitive feather structures. 4. The sources of nutrition and energy for the completion of keratinization during later developmental stages of feather germs are the supportive and the barb medullary cells and large stores of glycogen. 5. Keratohyalin granules are not precursors of feather keratin, since no such structures were observed in feather germs. 6. Two distinct modes of keratinization occur in feather germs. Keratinization in sheath cells is similar to that which occurs in mammalian epidermal cells. Barb and barbule cell keratinization resembles that of hair. 7. The basal lamina is probably involved in transport of synthetic material from the pulp cavity to the epidermal cells. The lamina may also provide mechanically strong connections between the feather germ and the dermis. It is suggested that desmosomal tonofilaments provide a framework which orients the synthesis of keratin. It is also suggested that the periderm granules provide mechanically weak areas in the sheath and facilitate the fragmentation of this structure.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 130 (1970), S. 216-233 
    ISSN: 1432-0568
    Keywords: Guinea-pig ; Placenta ; Syncytiotrophoblast ; Fetal capillaries ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurde elektronenmikroskopisch die reife Hauptplacenta von 31 Meerschweinchen untersucht. Die Ergebnisse zeigen, daß das Organ aus, drei morphologisch unterscheidbaren Gebieten aufgebaut ist: Interlobär- und, Randsyncytium, Läppchenperipherie und Läppchenzentrum. Zwischen diesen Zonen befinden sich noch zwei Übergangszonen, die die Eigenschaften der angrenzenden Hauptgebiete haben. Das Interlobär- und Randsyncytium enthält keine fetalen Capillaren, besitzt, aber teilweise ein sehr gut entwickeltes endoplasmatisches Reticulum mit zum Teil parallel angeordneten Zisternen sowie vielen Einschlüssen und verschiedenartigen vacuolären Gebilden. Nach dem Vorkommen dieser Bestandteile unterscheiden wir vier unregelmäßig verteilte Syncytioplasmazonen: a) mit vielen Vacuolen, b) mit vielen Filamenten, c) mit Vacuolen und Filamenten und d)— am häufigsten — Gebiete, die fast frei von derartigen Strukturen sind. Der Trophoblast besitzt an der den mütterlichen Lacunen zugewandten Oberfläche viele Mikrovilli und unterschiedliche polypartige Gebilde. Die Läppchenperipherie ist reich an fetalen Capillaren. Die Trophoblastbalken sind dünn und zeigen unterschiedlichen Organellengehalt. Oft kommen viele Vesikel vor. Das endoplasmatische Reticulum ist um die mitochondrien angeordnet. Das Läppchenzentrum zeigt häufig eine vacuolisierte, netzartige Struktur. Im Syncytiotrophoblast sowie in den Endothelzellen werden ausgedehnte endoplasmatische Zisternen gesehen. Die Oberfläche der fetalen Capillaren besitzt viele polypartige Gebilde. In funktioneller Hinsicht wird das Rand- und Interlobärsyncytium mit der Proteinsynthese in Zusammenhang gebracht. Trotz des Fehlens embryonaler Gefäße können sich hier Resorptionsvorgänge abspielen (positive ATP'ase-Reaktion). In der Läppchenperipherie überwiegt möglicherweise der Austausch der Atemgase und niedermolekularer Substanzen. Das Läppchenzentrum scheint wieder in Beziehung zum Proteinaustausch und zum Lipidumsatz zu stehen. Es gibt Hinweise, daß über alle Placentazonen hinweg eine lebhafte Cytoplasmaströmung erfolgt.
    Notes: Summary The mature main placenta of 31 guinea-pigs was investigated by means of electron microscopy. It is shown that the organ consists of 3 morphologically different regions, i.e. interlobar and marginal syncytium, lobular periphery and lobular centre. Between these regions there are 2 transitional zones having the morphological characteristics of both adjacent main regions. The interlobar and marginal syncytium is devoid of fetal capillaries However, in some parts it has an extremely developed endoplasmic reticulum, in parts of which the cisternae are arranged in parallel. In addition it has many inclusions and various vacuolated structures. According to the presence or absence of these structures four irregularly distributed syncytioplasma zones can be distinguished: a) containing large numbers of vacuoles, b) containing large numbers of filaments, c) containing vacuoles and filaments and d)— the main group —regions which are almost entirely free from these structures. On the surface facing the maternal lacunae the trophoblast has an abundance of microvilli as well as various polypoid structures. The lobular periphery is rich in fetal capilaries. The trabeculae of the trophoblast are thin and show a varying of organelles. Frequently large numbers of vesicles are observed. The endoplasmic reticulum is arranged around the mitochondria. The lobular centre frequently shows a vacuolized net-like structure. Extensive endoplasmic cisternae are observed in the syncytiotrophoblast as well as in the endothelial cells. The surface of the fetal capillaries shows an abundance of polypoid structures. From a functional point of view it seems likely that the interlobar and marginal syncytium is involved in protein synthesis. Despite the lack of fetal capillaries, absorptive processes can take place in this region. It is suggested that the main function of the lobular periphery is exchange of the respiratory gases and of substances of low molecular weight. The lobular centre seems to be associated with protein synthesis and lipid turnover.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 10 (1970), S. 276-282 
    ISSN: 1432-1106
    Keywords: Chlorpromazine ; Rabbit hypothalamus ; Glycogen ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The tuber cinereum of hypothalamus, cerebral cortex, cerebellar cortex and caudate nucleus of rabbits were examined under the electron microscope following intramuscular administration of chlorpromazine with special consideration of ultrastructural changes in amount and distribution of glycogen granules in their hypothalamus. In these regions, normal astrocytes and their processes contain glycogen granules diffusely scattered in the cytoplasm. In the neurons of the normal hypothalamus and cerebellar cortex, glycogen granules are seen in some presynaptic endings and distal parts of dendrites but not in the perikaryal cytoplasm. In the tuber cinereum of the hypothalamus, after chlorpromazine administration, abundant glycogen granules accumulate at the postsynaptic sites, especially in peripheric parts of dendrites, and clusters of glycogen granules appear in the perikaryal cytoplasm of the nerve cells. These findings are interpreted as an increase of glycogen in these cellular regions and the suggestion is made that chlorpromazine inhibits the glycolytic metabolism in the distal parts of dendrites, particularly at postsynaptic sites and in the perikarya of nerve cells of the hypothalamus.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 11 (1970), S. 387-391 
    ISSN: 1432-1106
    Keywords: Trifluoperazine ; Rabbit pallidum ; Glycogen ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Following administration of trifluoperazine (a psychotropic drug of the phenothiazine group), the brains of rabbits were examined by electron microscopy, with special reference to the glycogen changes in the globus pallidus, and were compared with the normal materials. After seven days' intramuscular injections of trifluoperazine (10 mg/kg/day), abundant glycogen granules were accumulated in dendrites of pallidum. Those animals showed extrapyramidal symptoms throughout the period. The administration of the drug might inhibit either neural activity or the glycolytic metabolism in dendrites of the pallidum.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 10 (1970), S. 1-16 
    ISSN: 1432-1106
    Keywords: Cerebral cortex ; Laminar lesions ; Degeneration ; Dendrites ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The morphological characteristics of dendrites in layers of the cerebral cortex above laminar lesions induced by ionizing particle irradiation have been studied in the striate field of rat at various survival times. Within two weeks following irradiation an increasing number of dendrites display unusual alterations inferred to be signs of degeneration. Degenerating dendrites can be characterized by a dense cytoplasmic matrix, disruption of mitochondria, presence of dense bodies, irregular outline and a marked alteration of the plasmalemma in its dimensions and staining properties. Some degenerating dendrites possess a large accumulation of dense subsynaptic material and are contacted by synapses with enlarged and altered synaptic clefts. A few dendrites contain extensive membranous whorls. Engulfment by reactive astrocyte processes is a common feature and often includes the presynaptic axonal knob, but only the degenerating dendrite has been observed within glial cytoplasm. The inference that the majority of degenerating dendrites in this material are apical dendrites of pyramidal cells suggests that either shaft synapses are common for these cells, protuberances may retract during degeneration, or spines are lost due to loss of afferent terminals.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 11 (1970), S. 528-538 
    ISSN: 1432-1106
    Keywords: Visual pathway ; Hypothalamus ; Arcuate nucleus ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Degenerating nerve fibres and boutons were searched with the aid of the electron microscope in the arcuate nucleus of rats 2–7 days after bilateral destruction of the retina. In the arcuate nucleus of the control animals as well as in the operated animals, 4 types of boutons were distinguished on the basis of vesicular contents and glial ensheathment. In the operated animals changes interpreted as degenerating were found in small myelinated axons and boutons of type II (boutons containing both synaptic and granular vesicles). The changes were similar to those described in the literature as the “dark” type of degeneration in experimentally interrupted axons and boutons. Similar changes were not found in the unoperated animals. The conclusion is reached, that a small number of fibres of the optic tract reach the arcuate nucleus to terminate here.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 316-345 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Histophysiology of median eminence ; Avian neurohypophysis ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les effets de l'adénohypophysectomie et de diverses sollicitations de l'axe hypothalamo-hypophysio-corticosurrénalien sur l'ultrastructure de l'Eminence Médiane (E.M.) ont été étudiés chez le Pigeon. 1. Chez le Pigeon entier, l'Eminence Médiane Caudale (E.M.C.) se distingue de l'Eminence Médiane Rostrale (E.M.R.) essentiellement par l'absence dans les deux couches les plus externes (couches palissadique et superficielle) de l'E.M.C. de granules de gros calibres (1600 à 1900 Å), la rareté de granules de diamètre moyen (1200–1400 Å) et la prédominance de petites vésicules à cœur dense de 600–800 Å. 2. La préhypophysectomie entraine: a) dans l'E.M.R. la quasi disparition de granulations dans les deux couches externes; b) dans l'E.M.C. la ≪vidange≫ de nombreux axones, mais un enrichissement relatif, parmi les granulations restantes, des granulations de gros calibre (1600–1900 Å) aux dépens des granules de plus petit calibre. 3. Un shock insulinique entraine des modifications du même ordre: a) déplétion des granules denses, limitée dans ce cas à la portion la plus antérieure des deux couches externes de l'E.M.R.; b) enrichissement relatif des granulations de moyen (1200–1400 Å) et de gros (1600–1900 Å) calibre dans l'E.M.C. avec, en plus dans l'E.M.C., un enrichissement en vésicules de type synaptique. 4. Un traitement à la métopirone produit un accroissement du nombre des granulations de moyen (1200–1400 Å) calibre dans les couches externes de l'E.M.R. et de l'E.M.C., et un enrichissement important de l'E.M.C. en vésicules de type synaptique. 5. Le traitement à la prednisolone conduit à un enrichissement très marqué des couches externes de l'E.M.R. en grains de 1200–1400 Å, et à un enrichissement des couches externes de l'E.M.C. en granulations de 1000 Å. Ces résultats sont discutés dans la perspective des régulations hypothalamo-corticotropes, particulièrement en ce qui concerne les granules de 1200–1400 Å.
    Notes: Summary The effects of adenohypophysectomy, and of several experimental interventions on the hypothalamo-pituitary-adrenal cortical axis have been studied in relation to the fine structure of the median eminence in the pigeon. 1. In control animals, the following morphological features of the caudal median eminence (C.M.E.) distinguish it from the rostral median eminence (R.M.E.): a) the absence in both external layers of the C.M.E. of large (1,600–1,900 Å) electron-dense granules, b) the presence in the C.M.E. of a small number of medium-size (1,200–1,400 Å) granules, and c) the predominance in the C.M.E. of small (600–800 Å) dense-core vesicles. 2. Adenohypophysectomy leads to: a) almost complete disappearance of electron-dense granules in both external layers of the R.M.E., and b) “emptying” of numerous axons and a relative increase in the number of large (1,600–1,900 Å) granules in the C.M.E. 3. Insulin shock produces modifications similar to those of adenohypophysectomy. The depletion of electron-dense granules from the axons is, however, restricted to the most anterior part of the R.M.E., and, in the C.M.E., the relative increase in the number of larger granules affects the 1,200–1,400 Å and the 1,600–1,900 Å size granules. 4. Metopirone enhances the number of medium-size (1,200–1,400 Å) granules in the external layers of both the R.M.E. and the C.M.E. and causes a significant increase in the number of synaptic-like vesicles in the C.M.E. 5. Prednisolone treatment leads to a marked enrichment of the external layers of the R.M.E. with 1,200–1,400 Å granules, and of the external layers of the C.M.E. with 1,000 Å granules. These results have been discussed with special reference to the hypothalamic control of the adrenocorticotropic function, especially reviewing the role of the 1,200–1,400 Å granules.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 451-472 
    ISSN: 1432-0878
    Keywords: Hemocytopoiesis ; Insects ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Mikroskopische Beobachtungen an normalen „phagozytären Geweben“ (im Sinne der älteren Autoren) entlang des dorsalen Diaphragmas der beiden Orthopteren-Arten Gryllus bimaculatus und Locusta migratoria zeigen übereinstimmend, daß diese Bildungen eine hematopoietische Struktur haben. Bei beiden Arten entwickeln sich die blutbildenden Stammzellen aus einer großen Anzahl sog. Retikularzellen mesodermalen Ursprungs, die den Retikularzellen der blutbildenden Gewebe der Vertebrata sehr stark ähneln. Wie bei den Vertebrata differenzieren sich bei den Insekten die Blutzellen in sog. isogenen Zellgruppen von gleichem Typus und gleichem Entwicklungsstadium. Die starke phagozytäre Neigung der Retikularzellen erklärt, warum die blutbildenden Gewebe der Orthoptera von den älteren Autoren als phagozytäre Organe angesprochen wurden. Die hämatopoietische Differenzierung der Retikularzellen in reife Blutzellen (Haemozyten) findet entweder in einem lockeren Gewebe entlang des dorsalen Blutgefäßes, wie bei Locusta, statt, oder im inneren mehrerer, an das Herz gebundener, hoch organisierter blutbildender Organe, wie bei Gryllus, die noch stärker an die klassischen Strukturen der Vertebrata erinnern. Wir beschreiben im einzelnen beide Strukturtypen, insbesondere bei Gryllus die Einteilung der Organe in einen Cortex, in dem sich die Blutzellen bilden, und eine Medulla, in welcher sich die reifen Haemozyten ansammeln können. Nach starken Blutverlusten zeigen besonders die blutbildenden Gewebe von Gryllus eine dramatische Stimulierung der Hämatopoiese an; die Punktion der hämatopoietischen Organe kann also ebenfalls experimentell nachgewiesen werden.
    Notes: Summary Microscopic observations of the normal “phagocytic tissues” (in the sense of the classic authors) of the dorsal diaphragm in the two Orthopterans Gryllus bimaculatus and Locusta migratoria unequivocally demonstrate the hematopoietic nature of these cellular accumulations. In the two species, the hematopoietic elements develop from a large number of so-called reticular cells of mesodermic origin, which resemble closely the reticular cells of the hematopoietic organs of Vertebrates. As it is the case in Vertebrates, the differentiation of the hematopoietic elements into mature blood cells occurs in the two Orthopterans also in isogenic cell islets. The phagocytic activity of the reticular cells explains the fact that these organs were classically considered in the Orthopterans as simple phagocytic organs. The hematopoietic differentiation of the reticular cells can occur either in a poorly organized, loose tissue located along the dorsal vessel, as is the case in Locusta, or in a group of highly organized hematopoietic organs, as in Gryllus, which resemble far more the classical hematopoietic structures of Vertebrates. We give a detailed description of both types of organization, especially of the subdivision in Gryllus, of the hematopoietic organs into a cortex, where the haemocytes differentiate, and a medulla, where they can accumulate. After severe hemorrhages, the hematopoietic organs of Gryllus show all the features of a dramatic stimulation of hematopoiesis; their function can thus be experimentally demonstrated.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 523-538 
    ISSN: 1432-0878
    Keywords: Ependyma ; Intracytoplasmic lipid bodies ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei erwachsenen Feuersalamandern und bei Salamanderlarven konnten in Ependymzellen sowie in subependymären Zellelementen des Gehirns und Rückenmarks zahlreiche rundliche intrazytoplasmatische Körper von starker Osmiophilie beobachtet werden, deren Durchmesser bis zu 12 μ betrug. Vereinzelt fanden sich diese voluminösen Gebilde auch im Cytoplasma von Satellitenzellen der Hirnnerven- und Spinalganglien. Das histochemische Verhalten und das Ultrastrukturbild der intrazytoplasmatischen Körper sprechen dafür, daß sie hauptsächlich aus Lipiden bestehen. Ihre funktionelle Bedeutung konnte bisher nicht eindeutig geklärt werden.
    Notes: Summary The ependymal and subependymal cells of the ventricular system and the central canal in adult und larval salamanders contain numerous unusually large intracytoplasmic osmiophilic spherical bodies with a diameter of up to 12 μ. Sporadically the bodies are found within satellite cells of peripheral ganglia. Histochemical and ultrastructural examination suggests that the bodies consist mainly of lipids. Their functional significance is unknown.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 384-397 
    ISSN: 1432-0878
    Keywords: Heart muscle cells ; Electron microscopy ; Quantitative ; Asphyxia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Normale und hypoxische Herzmuskelzellen aus der Wand des linken Ventrikels der Ratte wurden quantitativ-morphologisch anhand von elektronenmikroskopischen Längsschnitten nach Perfusionsfixierung untersucht. In normalen Zellen waren alle Myofibrillen relaxiert, die mittlere Sarcomerlänge betrug 2,2 μm. Die Schnittfläche wurde zu 55% von Myofibrillen, zu 27% von Mitochondrien und zu 18% von Grundplasma und Reticulum eingenommen. Die zwischen den Myofibrillen liegenden Mitochondrien waren längsoval und im Mittel 2,3mal so lang wie breit. Es bestand kein Unterschied zwischen subendokardial und subepikardial gelegenen Zellen. 10 min nach Erstickung der Tiere waren in den sonst unauffälligen Muskelzellen die Glycogengranula vermindert. Nach 20 min führte die Hypoxie zu einer Zunahme der relativen Schnittfläche der Mitochondrien um etwa 16% und zu einer beginnenden Kontraktur der Myofibrillen (Sarcomerlänge 2,0 μm). 20 min Hypoxie in Hypothermie (25–30°C intrathorakal) veränderte die normale Zellstruktur dagegen kaum. Wenn die Herzen während der 20 min dauernden Hypoxie in Normothermie mit einer procainhaltigen sauerstoff- und glucosefreien Blutersatzlösung durchspült wurden, waren die Myofibrillen relaxiert, die Schwellung der Mitochondrien dagegen wurde nicht reduziert. 30 min nach Erstickung wurde die Kontraktur stärker (Sarcomerlänge 1,7 μm). Nach 60 min bildeten sich Superkontraktionsknoten, einzelne Myofibrillen waren in Höhe der I-Bänder unterbrochen. Die Cristae der Mitochondrien wichen auseinander, die Schnittfläche der Mitochondrien hatte um 27% zugenommen. Während in Normotherapie eine Asphyxie des Tieres bereits nach 10 min die Herzmuskelzellen funktionell schwer schädigt, ist die Schädigung morphologisch erst nach 20 min eindeutig. Das bedeutet, daß für die elektronenmikroskopische Präparation eine Hypoxie von unter 10 min bedeutungslos ist. Hinsichtlich der morphologischen Manifestationszeit für die Unterbrechung der Sauerstoffversorgung stimmen unsere Befunde an Herzmuskelzellen gut mit vergleichbaren Angaben an Leberzellen überein.
    Notes: Summary In heart muscle cells of the left ventricle of rats the distribution of cell organelles and their reaction to hypoxia were investigated by electron microscopy. In normal hearts fixed by perfusion with aldehydes, the mean sarcomere length was 2.2 μm. 27% of the longitudinal sectional area was occupied by mitochondria, 55% by myofibrils and 18% by sarcoplasmic reticulum and ground plasm. The mitochondria situated in rows between the fibrils were oval and measured 2.3 times more in length than in width. There was no difference between cells from subendocardial and subepicardial regions. 10 min hypoxia (complete occlusion of the trachea) did not affect the appearance of muscle cells but diminished the number of glycogen granules. After 20 minutes the area occupied by mitochondria was increased by 16%, the mitochondria between the myofibrils were more spherical and only 1.5 times longer than wide. The sarcomeres shortened to 2.0 μm. With hypothermia (25–30°C) hypoxia of 20 minutes duration did not affect the cell structure. Perfusion of the heart by a saline solution, which contained procaine but neither oxygen nor glucose, for 20 minutes prevented shortening of the sarcomeres but not swelling of the mitochondria. 30 minutes after occlusion of the trachea the myofibrils shortened to a sarcomere length of 1.7 μm. After 60 minutes irregularly and excessively contracted myofibrils appeared and some sarcomeres were interrupted at the level of the I-bands. In some of the swollen mitochondria the cristae were widely separated. The increase of the area occupied by mitochondria was 27%. Asphyxia affects heart muscle cells severely with respect to function within 10 min, but morphologically it takes 20 min before a definite effect can be noticed. As to the time after which lack of oxygen is manifested morphologically, our results are consistent with findings in liver cells.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 431-449 
    ISSN: 1432-0878
    Keywords: Contractile ring ; Cytokinesis ; Cell division ; Cytochalasin B ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Techniques of individual cell selection and precise ultramicrotomy have been employed to demonstrate that the contractile ring of cleaving HeLa cells is a transitory cytoplasmic organelle of distinctive fine structure and location. The contractile ring is an uninterrupted annulus encircling the equator of dividing cells exactly where the cleavage furrow forms. It is about 10 microns wide, up to 0.2 microns in thickness, and is composed exclusively of circumferentially aligned thin filaments 40–70 Å in diameter. Contractile ring filaments appear to be associated with the overlying plasma membrane. Controlled experiments with a mold metabolite (cytochalasin B) reveals that within a few minutes the drug abolishes the ability of HeLa cells to undergo cytokinesis. Cytochalasin B seems to decompose the contractile ring. It has no other clearly identifiable effects on other cell structures, notably the mitotic apparatus. Cytochalasin B is the only drug known which selectively inhibits cytokinesis in animal cells. In conclusion, the contractile ring is the most likely organelle responsible for cytokinesis in HeLa cells. Similar organelles probably occur in all cleaving animal cells. Successful cleavage depends upon the structural and functional integrity of the contractile ring.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 153-165 
    ISSN: 1432-0878
    Keywords: Reptiles ; Skin ; Keratin ; Electron microscopy ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The different patterns of keratin formation that have evolved in the class Reptilia are all variations of a common process. In Squamata (snakes and lizards), a sequence of layers composed of α or β keratin is formed periodically, after which the old epidermal generation is shed. In Chelonia (turtles and tortoises), the epidermis of the shell is composed of only β keratin, whereas the skin of the neck and leg is composed exclusively of α keratin. Molting in toto does not occur and shedding is a continuous process comparable to that in avian and mammalian epidermis. In Crocodilia (crocodiles, caimans, alligators) there is only a single layer of cornified cells, but the composition of the layer varies in different parts of the scale. The hinge regions have many of the morphological characteristics of α and β keratin whereas the center resembles β keratin. The living cells beneath contain accumulations of keratohyalin. There are four ultrastructural characteristics of a keratinized α layer: 1) cellular outlines remain distinct, 2) a thickened plasma membrane forms during keratinization, 3) 80 Å filaments embedded in an amorphous matrix can be seen, and 4) PAS-positive material accumulates in extracellular spaces between the desmosomes. The β layer exhibits none of these features. Instead the cells more or less (depending on species) coalesce into a compact layer which becomes attenuated in the hinge regions. A 30 Å filament pattern can be seen. The mesos layer of squamates resembles the hinge region of crocodilians, exhibiting a combination of the characteristics of both α and β keratin.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 309-321 
    ISSN: 1432-0878
    Keywords: Red nucleus ; Substantia nigra ; Neuroglia ; Pericytes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of perivascular and perineuronal cells in the substantia nigra and red nucleus was studied in Nissl, silver carbonate, and electron microscopic preparations. In light microscopic preparations of the red nucleus and substantia nigra oligodendrocytes and astrocytes are located adjacent to blood vessels and nerve cells. Pericytes are also found adjacent to blood vessels. Scattered perineuronal oligodendrocytes and astrocytes are present in the magnocellular portion of the red nucleus and in the substantia nigra, whereas a distinguishing morphological feature of the parvocellular portion of the red nucleus is the clustering of perineuronal oligodendrocytes around a single neuron. In the present electron micrographs of the red nucleus and substantia nigra oligodendrocytes are separated from the vascular basement membrane (basal lamina) by astrocyte processes and therefore are not truly perivascular. Pericytes are easily identified by the basement membrane which encompasses their cell bodies and processes. Characteristic of the neuropil in the red nucleus are astrocytic processes that approximate dendrites. In contrast, astrocytic processes in the substantia nigra rarely contact dendrites which are covered by a mosaic of synaptic endings. A “third type of neuroglial element” is also present in the neuropil of the substantia nigra and the red nucleus.
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  • 14
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    Cell & tissue research 108 (1970), S. 324-338 
    ISSN: 1432-0878
    Keywords: Human myocardium ; Innervation ; Nerve endings ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscope studies of axons, distributing singly or in small bundles in the human ventricular and atrial myocardium, indicate a few per-cent of the axon profiles to be significantly large in diameter (1.5–3.0 μ). They are characteristically packed with a profuse number of mitochondria along with large granular vesicles, glycogen rosettes, lysosomic bodies; and some of them terminate on a “specific terminal cell” (Knoche and Schmitt). These mitochondria-rich, large axons are assumed to be terminal portions of the cardiac afferents. About half of the axons encountered in the ventricle and 2/3 in the atrium are non-vesiculated, usually less than 0.5 μ. in diameter. The varicosities containing numerous vesicles are mostly 0.5–1.5 μ in diameter and are assumed to be terminal portions of the cardiac efferents. The ratio between the number of axon profiles containing small granular vesicles and that of axon profiles containing agranular vesicles without small granular vesicles is 2∶1 in the ventricular myocardium and 1∶1.7 in the atrial myocardium.
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  • 15
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    Cell & tissue research 109 (1970), S. 33-45 
    ISSN: 1432-0878
    Keywords: Testis ; Interstitial cells ; Reptiles ; Hormone Production ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'ultrastructure des cellules interstitielles du testicule de Lacerta vivipara a été étudiée entre le printemps et l'automne pendant deux années. Le retioulum endoplasmique lisse, et les mitochondries à crêtes tabulaires sont les organites les plus remarquables comme dans les autres cellules productrices de stéroïdes, mais les liposomes et l'appareil de Golgi sont bien représentés aussi. Les variations ultrastructurales les plus significatives apparaissent entre le printemps et le début de l'été. Au printemps, alors que les caractères sexuels secondaires sont hypertrophiés, un système remarquable de vésicules et de vacuoles se développe à partir du reticulum et probablement aussi du Golgi. Au début de l'été, lorsque les caractères sexuels secondaires sont atrophiés, les vacuoles sont moins nombreuses et le reticulum forme un réseau dense de tubules typiques, souvent étroitement associés aux liposomes; les crêtes mitochondriales sont gonflées. Ces images sont discutées en fonction de l'activité saisonnière d'élaboration d'hormones. L'hypertrophie des systèmes membranaires au printemps correspond probablement à la production ou (et) à l'excrétion des hormones androgènes. Au début de l'été, la cellule n'élabore pas d'androgènes, mais n'est peut-être pas complètement inactive: elle pourrait stocker des précurseurs hormonaux.
    Notes: Summary Interstitial cells of the testis of Lacerta vivipara have been studied electronmicroscopically in animals obtained between spring and autumn. Smooth endoplasmic reticulum and mitochondria with tubular cristae are the most prominent organels, lipid droplets and Golgi apparatus being also well developed. The most significant ultrastructural changes occur between spring and the beginning of summer. In spring, during the hypertrophy of secondary sexual characters, a conspicuous system of vesicles and vacuoles originates from the smooth endoplasmic reticulum and probably also from the Golgi apparatus. At the beginning of summer, when secondary sexual characters are atrophied, vacuoles are less prominent and the smooth endoplasmic reticulum consists of a dense network of typical tubules, often closely associated with the lipid droplets; the cristae of the mitochondria are swollen. These ultrastructural findings are discussed in relation to the production of hormones. The hypertrophy of membrane systems in spring corresponds presumably to production or (and) release of androgen hormones. In the beginning of summer the cell does not produce androgens, but probably is not completely inactive: it may store precursors of hormones.
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  • 16
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Gastropoda ; Fluorescence ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Durch Vergleich fluoreszenz- und elektronenmikroskopischer Untersuchungen am zentralen Nervensystem von Planorbarius corneus L. wird nachgewiesen, daß in den Schlundringganglien Neurosekretzellen vorkommen (Nachweis mit Pseudoisocyaninchlorid), die mit Nervenzellen nicht identisch sind, die durch ihren hohen Gehalt an biogenen Aminen auffallen (Nachweis durch die Methode von Falck und Owman, 1965). Es können daher im Schlundring von Planorbarius corneus peptiderge und aminerge Neurosekretzellen unterschieden werden. Die PSC-positiven Neurosekretzellen enthalten elektronendichte Elementargrana und die aminergen Neurone „dense-core“ Vesikel. Der Nachweis biogener Amine in einigen Nervenzellen von Planorbarius corneus spicht für deren chemische Identität mit Transmittersubstanzen, ihre hohe Konzentration aber für eine Abgabe in die Körperflüssigkeit.
    Notes: Summary The neurosecretory system of the snail Planorbarius corneus has been investigated by fluorescence and electron microscopy. With the fluorochrome Pseudoisocyanin the established neurosecretory system in the cerebral ganglia and single neurosecretory cells in the other ganglia show an intensive yellow fluorescence. Electron micrographs reveal the presence of electron dense granules (elementary granules) in the pericarya and the axons of neurones which have the same localisation in the ganglia as the pseudoisocyanin-positive cells. The fluorescence technique for biogenic amines produces yellow and green fluorescence within neurons and in the neuropil and nerves. The fluorescence obtained in determinable areas and neurones correlates well with the electron microscopic location of “dense-core” vesicles within the pericarya and axons of cells in even the same areas. It is discussed, that in this animal both types of cells are so-called “neurosecretory cells”, because the high content of elementary granules in the “peptidergic neurosecretory cells” and of “dense-core” vesicles in the “aminergic neurosecretory cells” is an indication for secretion of these products in neurohaemal areas (circulatory channels).
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  • 17
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    Cell & tissue research 110 (1970), S. 321-335 
    ISSN: 1432-0878
    Keywords: Thymus ; Lymphocyte ; Analysis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thymic small lymphocytes of dd-mice were qualitatively and quantitatively studied by electron microscopy. Differences in fine structure were revealed between cortical and medullary small lymphocytes. Cortical small lymphocytes are rounded in cell outline with a round nucleus. The cytoplasm surrounding the nucleus as a narrow rim is scanty and appears relatively dense due to an abundance of free ribosomes. The cell organelles are not well developed. On the other hand, medullary small lymphocytes are more irregular in shape with uneven cell membranes. Their nuclei are also more irregular in outline with frequent infoldings of the nuclear membrane. The cytoplasm is more abundant and paler with less numerous ribosomes. The cell organelles are better developed. Quantitative analysis was made of both cortical and medullary small lymphocytes by means of the point counting method. The nuclei of both cortical and medullary small lymphocytes are almost the same in size (a diameter of 4.9 μ). The cell sizes are different between cortical and medullary lymphocytes: cortical small lymphocytes with a diameter of 5.5 μ were smaller than medullary ones with a diameter of 6.4 μ. Cortical small lymphocytes are very sensitive to the destructive effects of hydrocortisone, whereas the medullary ones are resistant. Periarteriolar lymphoid sheath in the splenic white pulp, which is known to be a thymus-dependent area, contains small lymphocytes that were similar in cytological details to medullary small lymphocytes of the thymus. In the light of the recent knowledge about a recirculating long-lived small lymphocyte pool, it appears probable that medullary small lymphocytes represent a contribution to the pool and that small lymphocytes with a long life span can be cytologically identified by electron microscopy.
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  • 18
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    Cell & tissue research 110 (1970), S. 386-400 
    ISSN: 1432-0878
    Keywords: Autonomic nerves ; Perineurial sheath ; Termination ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Perineurium kleiner Mesenterial- und Trachealnerven besteht aus einer Lage platter, beiderseits von einer Basalmembran umschlossener Zellen. Im Bereich von Abzweigungen kleiner Nerven bildet das Perineurium ein gekammertes, stark kollagenfibrillenhaltiges Hüllensystem. Die Endigung der einschichtigen Perineuralhülle hat die Form einer in das Interstitium geöffneten Röhre. Im ansatznahen Drittel des Mesenteriums und in der Schleimhaut der Trachea wurden ausschließlich perineuriumfreie Nerven gefunden. Es liegt nahe, diese perineuriumfreien Bezirke zu funktionellen Endräumen zusammenzufassen. In perineuriumfreien Bezirken wird das gehäufte Auftreten von Nervenauftreibungen mit Vesikeln, die Transmittersubstanz enthalten, und Mitochondrion beobachtet. Die erhobenen Befunde werden mit den an dickem Perineurium gewonnenen Ergebnissen verglichen und diskutiert.
    Notes: Summary The perineurial sheath of small tracheal and mesenteric nerves consists of a layer of flat, basement membrane coated cells. Its ramifications form a complicated layered system, interspersed with collagen fibrils. The tube formed by the thin perineurial sheath ends openly, which affords communication between the respective interstitial spaces. The mesenteric nerves close to the duodenum as well as the nerves in the vicinity of the tracheal epithelium show no perineurial sheath. Peripheral nerves lacking a perineurial layer seem to be close to their terminations. Their fibers show varicosities containing transmitter vesicles and mitochondria with longitudinally orientated cristae. Our results are compared with those found in thick, multilayered perineurial sheaths and functional differences are discussed.
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  • 19
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    Cell & tissue research 111 (1970), S. 51-63 
    ISSN: 1432-0878
    Keywords: Purkinje cell ; Golgi apparatus ; Dendrites ; Differentiation ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of postnatal differentiation of the Golgi apparatus, the nucleus, the perikaryon, and the dendrites was studied in Purkinje cells of the rat cerebellum for 30 days after birth using histochemical, histological, and electron microscopic methods. The Golgi apparatus during differentiation undergoes morphological and positional changes. From the 1st to 7th postnatal day, the Golgi apparatus is found in a supranuclear position, and is connected with the axes of differentiating primary dendrites by beam-like processes. From days 8 to 11 this connection disappears, and most of the Golgi apparatus assumes a lateronuclear and infranuclear position. After the 11th or 12th day, the Golgi apparatus is found in perinuclear and peripheral cytoplasmic positions. The formation of granular endoplasmic reticulum occurs in the vicinity of the perinuclear Golgi apparatus. The differentiation of cell and nuclear forms requires approximately 20 days. The morphological changes of differentiation are discussed in relation to the participation of the Golgi apparatus in the differentiation of dendrites and in the formation of the granular endoplasmic reticulum.
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  • 20
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    Cell & tissue research 111 (1970), S. 149-159 
    ISSN: 1432-0878
    Keywords: Rabbit ovum ; Fertilization ; Pronucleus ; Cleavage ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fertilized ova were flushed from the Fallopian tubes of New Zealand White rabbits at 15 to 30 hours after mating and examined with the electron microscope. Between 21 and 22 hours intact pronuclei with extensive interdigitation of apposing surfaces were found in most ova. In some, an appearance suggesting internuclear communication was observed. In other ova disrupted pronuclear membranes surrounded centrally placed chromosome aggregates. In still others an advanced cleavage furrow was already present. By 23 hours all ova were in the two-cell stage.
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  • 21
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    Cell & tissue research 103 (1970), S. 382-397 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Newborn rat ; Stereology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the zona glomerulosa and the zona fasciculata of the newborn rat adrenal cortex has been studied by the light and the electron microscope. The ultrastructural features of the cells of both zones are described. By morphometric methods the relative volumes occupied by mitochondria and by lipid droplets have been evaluated. The “membrane profile concentration” (i.e. the average number of microns of smooth reticulum profiles per square micron of cytoplasm) has been also measured. The most significant ultrastructural differences between the cells of the two zones concern the mitochondria, the lipid droplets, and the smooth endoplasmic reticulum. The mitochondria show typical lamellar cristae in the elements of the zona glomerulosa, and vesicular cristae in the cells of the zona fasciculata. They occupy the same relative volume in both elements. The lipid droplets and the smooth reticulum show no qualitative differences in the two cell types, but they are found in a larger amount in the cells of the zona fasciculata. These ultrastructural findings are discussed in relation to the numerous biochemical data, suggesting that the cells of the zona glomerulosa are resting elements at birth, while the cells of the zona fasciculata are active steroid-secreting elements already in late gestation.
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  • 22
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    Cell & tissue research 103 (1970), S. 398-409 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Newborn rat ; Electron microscopy ; Lysosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structural changes of the zona juxtamedullaris of the rat adrenal cortex at birth, have been examined by the light and the electron microscope. In this zone clusters of medullary cells lying among the strands of cortical tissue were observed. In the inner portion of the zona juxtamedullaris two types of adrenocortical cells were found: light and very-dark cells. The latter are smaller than the light cells and are always in close connection with the medullary tissue. The ultrastructural features of the very-dark cells suggest that these elements are in degeneration. This finding supports the hypothesis that at birth there is a partial degeneration of the rat zona juxtamedullaris, i.e. the zone corresponding to the “fetal zone” of some mammalian species. It is proposed that in all mammalian species at birth there is a partial regression of the zona juxtamedullaris and that the regression of the “fetal zone” is only the quantitative increase of this phenomenon. This hypothesis is discussed in relation to numerous data demonstrating that there are enzymatic conditions in the rat during fetal life, which permit a discrete hypertrophy of the adrenal cortex.
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  • 23
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    Cell & tissue research 103 (1970), S. 341-350 
    ISSN: 1432-0878
    Keywords: Catecholamines ; Brain ; Insects ; Electron microscopy ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the brain of the cockroach Periplaneta americana, the beta lobes of the corpora pedunculata respond with an intense positive reaction to a specific fluorescence histochemical method for catecholamines. The fluorescence reaction disappears completely after prolonged treatment of the cockroaches with reserpine. An ultrastructural examination of the beta lobes in formaldehyde-glutaraldehyde-osmium fixed preparations reveals the presence of two types of fibres: 1) Fibres and nerve endings containing small clear vesicles and sligthly larger vesicles with a semi-dense content. The appearance and size distribution of these vesicles ist not affected by treatment with reserpine. 2) Fibres containing larger and denser vesicles, but practically no clear vesicles. The size distribution of these dense vesicles is only slightly affected by treatment of the cockroaches with reserpine. If brain slices are incubated in a medium containing noradrenaline or α-methyl-noradrenaline and fixed in permanganate, small vesicles with electron-dense central cores show up, similar to those which have been described in vertebrate adrenergic nerve fibres (“small granular vesicles”). They are confined to one of the two types of fibres (a and b) visible in these preparations, namely to type b, whose correspondence with type 2 fibres of formaldehyde-glutaraldehyde-osmium fixed preparations is discussed.
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  • 24
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    Cell & tissue research 104 (1970), S. 345-357 
    ISSN: 1432-0878
    Keywords: Photoreceptor ; Lumbricus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Feinstruktur der Photorezeptorzellen in der Epidermis, in kleineren Nervenästen und im Zerebralganglion von Lumbricus terrestris wurde untersucht. Das Vorhandensein eines zentralen, intrazellulären Lumens (Phaosom), das mit Mikrozotten gefüllt ist, erinnert in der Struktur der Photorezeptorzelle des Regenwurms an Lichtsinneszellen von Hirudo. Außer Mikrozotten findet man im Phaosom einige Zilien vom Typ 9×2+0; solche Zilien sind von den Mikrozotten strukturell unabhängig. Durch eine Aufzweigung des Phaosoms in kleinere Buchten, die tief in das umgebende Zytoplasma eindringen, erhält es ein labyrinthartiges Aussehen. Glatte Zisternen in Gruppen von 2 bis 5 wurden oft um das Phaosom im Zytoplasma beobachtet. Charakteristische Bestandteile der Zelle sind noch Vesikel und Vakuolen, die mit einer Substanz von wechselnder Elektronendichte gefüllt sind. Die Photorezeptorzellen werden von Gliazellen und Gliafortsätzen umgeben, die an vielen Stellen die Zelloberfläche tief einstülpen (Trophospongium).
    Notes: Summary Photoreceptor cells in the epidermis and nerve branches of the prostomium and in the cerebral ganglion of Lumbricus terrestris were investigated with the electron microscope. The photoreceptor cell is similar to the visual cell of Hirudo by having a central intracellular cavity (phaosome) filled with microvilli. Besides microvilli, several sensory cilia can also be found in the phaosome but they are structurally independent of the microvilli. A gradual branching of the phaosome cavity into smaller cavities makes its sectional profile extremely labyrinthic. Flattened smooth-surfaced cisternae in stacks of 2 to 5 are frequently observed around the phaosome. Characteristic constituents of the cytoplasm are vesicles and vacuoles filled with a substance of varying density. The photoreceptor cell is covered by glial cells or by their processes which at many places deeply invaginate the cell surface (trophospongium).
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  • 25
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    Cell & tissue research 105 (1970), S. 176-187 
    ISSN: 1432-0878
    Keywords: Ependyma ; Salamandra maculosa ; Neuronal elements ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Zwischen den eigentlichen Ependymzellen des Zentralkanals adulter Feuersalamander kommen amphorenartig gestaltete Elemente vor, die sich aufgrund ihrer synaptischen Kontakte mit Axonendigungen als Neurone identifizieren lassen. Diese intraependymalen Nervenzellen weisen einen apikalen Fortsatz auf, der sich mit einer warzen- oder knotenförmigen Protrusion in das Lumen des Zentralkanals erstreckt. Die Protrusion ist gewöhnlich mit stereozilienartigen Ausläufern besetzt. Die funktionelle Bedeutung der beschriebenen neuronalen Elemente konnte bisher nicht geklärt werden.
    Notes: Summary The ependyma of the canalis centralis of adult salamanders was examined by electron microscopy. Between the ependymal cells occur amphora-like elements identifiable as neurons by their synaptic contacts with axon terminals. These intraependymal nerve cells exhibit an apical outgrowth extending into the lumen of the canalis centralis with a wart-like or knob-like protrusion. The latter usually bears extensions resembling stereocilia. The functional significance of the neuronal elements is still unknown.
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  • 26
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    Cell & tissue research 105 (1970), S. 515-525 
    ISSN: 1432-0878
    Keywords: Cuticle ; Rotifer ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies of developing Asplanchna brightwelli embryos support the following hypothetical scheme of cuticle formation. First the external hypodermal membrane invaginates, and deposition of a dense intracellular layer commences next to this membrane. Then the rough endoplasmic reticulum synthesizes fibrous protein which is transferred to the Golgi complex. Here polysaccharide is synthesized and added to the protein, and the resulting filamentous complex is enclosed in large irregularly shaped vesicles which bud off from the Golgi elements. Maturation of the filamentous material to condensed cuticle material occurs as the vesicles move to the invaginations. Each vesicle fuses with an invagination, thus forming a hypodermal bulb; then the cuticle material is discharged through the neck of the bulb to its extracellular location. After the bulbs are formed, new, smaller, spherical vesicles begin to bud off from the Golgi elements. They too contain the filamentous complex which is refined to condensed cuticle material as the vesicles near the bulb. These vesicles fuse with the hypodermal bulbs contributing the cuticle and membrane necessary for the growth of the hypodermis of the embryo and newborn animal. Ruthenium red staining has confirmed that the cuticle consists of glycoprotein.
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  • 27
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    Documenta ophthalmologica 28 (1970), S. 191-200 
    ISSN: 1573-2622
    Keywords: Acomys Cahirinus ; Retinal capillary basement membrane width ; Age modifications ; Electron microscopy ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L étude morphométrique des capillaires rétiniens de souris à piquants de différents âges (3, 6, 12 et 36 mois) a démontré un épaississement des membranes basales et un élargissement des diamètres capillaires en fonction de l'âge et, en outre, l'existence d'une corrélation positive entre ces deux paramètres.
    Abstract: zusammenfassung Die morphometrische Untersuchung von Retinakapillaren verschieden Alter (3, 6, 12 und 36 Monate) Stachelmäuse (Acomys cahirinus) ergab eine altersabhängige Zunahme sowohl der Basalmembrandicke als auch des Kapillardurchmessers. Zudem wurde eine positive Korrelation zwischen diesen beiden Parametern gefunden.
    Notes: Abstract Specimens of four age groups of non-diabetic, non-obese spiny mice (Acomys Cahirinus), 3, 6, 12, 36 months old respectively, were examined for changes in retinal capillaries. Morphometrical evaluation of electron-micrographs allowed to conclude that capillary basement membrane width, as well as capillary diameter, increase during ageing: the values in the four different age groups were found to be 656, 925, 1098, 1772 Ångström units for the average basement membrane width, and 3,72, 4,08, 4,58, 5,60 microns for average capillary diameter. The differences between the age groups proved to be significant at a p 〈 0,01. Furthermore, a positive correlation between capillary diameter and capillary basement membrane width seems to be highly probable.
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  • 28
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 3-9 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The skin of Bagarius bagarius (Ham.) is devoid of scales but is rough due to the presence of numerous pentagonal epidermal elevations, which are separated by deep furrows at regular intervals. These elevated pentagonal regions of the epidermis are covered by dead cornified cells in the form of caps. As the old cap goes off a new one is formed by the death of the underlying epidermal cells. The middle layer of the epidermis is mainly composed of well defined polygonal cells. Their cytoplasm is granular in nature and give reactions for protein bound sulphydryl groups. The stratum germinativum is composed of two types of basal cells, the columnar cells and the spherical cells.The flask shaped mucous glands are restricted to the epidermal furrows and secrete either neutral or acidic mucopolysaccharides. Certain large specialysed granular cells are found in the epidermis which are grouped around the taste buds. These specialysed cells may be the photocytes.Two layers of the dermis can be distinguished - the relatively thin stratum laxum and the thick stratum compactum. Dermal papillae mainly support the taste buds. The pigment cells are arranged in two layers in the dermis.The subcutis is composed of loose connective tissues, richly infiltrated with the fat cells, nerves and blood capillaries.
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  • 29
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Electron microscope studies on Tylocephalum metacestodes embedded in the tissues of the oyster, Crassostrea virginica, have revealed that the tegument of the larval tapeworm is comprised of an external and an internal level which are partially separated by a basal lamina and two layers of muscles. The outer tegumentary level is comprised of an anucleate, cytoplasmic syncytium in which are embedded large and small vesicles and mitochondria. Surfacial hooks are also embedded therein. The internal level is comprised of relatively large discrete cells including mitochondria, rough endoplasmic reticulum, and large and small vesicles. These cells are intermittently connected with the external level by cytoplasmic bridges.Arising from the external level are unusual microvilli each of which terminates as a spherical vesicle. The stem of each microvillus is covered by a unit membrane which is continuous with that overlaying the body surface. In addition, each microvillus includes an external layer of medium electron density, a medial layer of intense electron density, and a core of heterogenous, medium electron density. These structures may be intertwined and bundles can be observed at the light microscope level as fibril-like projections from the parasite's body surface. One of their possible functions may be to prevent intimate contact between the encapsulating fibers of host origin and the parasite's body surface. In addition, the contraction and distention of the circular muscles result in microvillar movement which may keep the surrounding host fluids, including those of nutritional importance to the parasite, in a state of flux thus hypothetically permitting more uniform uptake.The abundance of vesicles in the syncytial external level of the tegument appears to be characteristic of the more primitive marine cestodes belonging to the orders Trypanorhyncha and Lecanicephala.
    Additional Material: 11 Ill.
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  • 30
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 31
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Sequential patterns of cuticle deposition and “melanization” in the imaginal cuticle of Sarcophaga argyrostoma in parts of the body darkening before or after emergence are examined on a histological basis. The patterns in the cuticles examined range from a simple absence of “melanization” to a complex of histological changes involving “melanization” and deposition. Ultrastructural changes in the post-emergent cuticle of Sarcophaga bullata during the hardening and darkening process and cuticle deposition are described.
    Additional Material: 2 Ill.
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  • 32
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 151-161 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Morphological study of Corynosoma hamanni (Linstow, 1892) was continued with the intention of presenting a morphological description of a single, well defined species.Sexual dimorphism is exhibited in body size, males being larger, and in body spination. Trunk spines are arranged in a pattern similar to that of Corynosoma semerme and do not extend onto the bursa as reported in the original description of C. hamanni.Two testes, located side by side, are followed by six cement glands arranged in two groups of three glands each. One group is behind each testis. Cement ducts from glands on the right side cross over Saefftigen's pouch and join to form a reservoir on the left side; ducts from cement glands on the left side fuse forming a second reservoir. The two cement reservoirs unite before emptying into the ejaculatory duct. The bursa possesses rays on its outer wall and numerous, well-developed papillae in the muscular cap.In females, a ruptured genital ligament sac is attached partially on the dorsal wall of the uterine bell and partially on a column of six cells projecting from the bell's base into its lumen. Postericrly the dorsal wall of the bell has two protruding pockets behind which occur ten cells composing the selector apparatus. Dorsal openings into the pseudocoelom occur on either side of the uterine bell. Two muscular tubes conduct eggs into the uterus. Female C. hamanni frequently form genital vestibules in which the genital pore occurs along the dorsal wall and not, as reported for some species, at the anterior tip.Eggs of C. hamanni possess four membranes rather than the previously reported three.
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  • 33
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A seasonal study of the seminal vesicles in relation to that of the testes had been conducted in the catfish, H. fossilis. The annual reproductive cycle of the catfish has been divided into (i) Preparatory period (February-April), (ii) Prespawning period (May-June), (iii) Spawning period (July-August) and (iv) Postspawning period (September-January). Testes exhibit initiation of spermatogenesis in the mid-preparatory period, but significant increase in weight of the testes accompanied by active spermatogenesis occurs during the prespawning period. In the spawning period, the testes are maximally enlarged and their seminiferous tubules are packed with spermatozoa. Following spawning, the testes gradually regress in the postspawning period. The seminal vesicles show initiation of secretory activity during the preparatory period but their recrudescence lags behind that of the testes by about a month. The seminal vesicles attain maximum weight and secretory activity during the spawning period. Thereafter, the seminal vesicles regress precipitously and sooner than the testes.The histochemical and biochemical studies on the seminal vesicles indicate that the secretion contains mucoproteins, acid mucopolysaccharides, primary proteoses, besides traces of phospholipids and native proteins.
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  • 34
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 247-255 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During cleavage of honeybee eggs two peaks arise in the longitudinal distribution pattern of the periplasm which coincide with the site of the differentiation center and the site of a mesodermal center. A very similar pattern is exhibited by the dorsal plasmstrip, a narrow band of thicker periplasm which is formed during cleavage along the dorsal midline of the egg. The present paper describes the development of the dorsal plasmstrips of two inbred strains of honeybees during early cleavage stages. Differences between the two strains reside in the total size of their dorsal plasmstrips and in the timing of the formation of the anterior peak which coincides with the site of the differentiation center. The bearing of these findings upon embryological studies is discussed.
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  • 35
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The “cuticle,” which revests the starfish tube foot, has been studied by electron microscopy and the findings correlated with histochemical observations.The “cuticle” is composed by two distinct zones; an outer zone including numerous microvilli, which extend from the inner zone into and through a fibrillar substance distinctly organized in two layers. These microvilli protrude slightly beyond the outer surface, where their tips give rise tonumerous extremely delicate fibrils. The second inner zone, of quite variable thickness and condensation of material, presents a coarser fibrous matrix where organelles and inclusions can be found.The whole cuticular complex does not derive from the majority of the epithelial cells, but is probably an extension of a special kind of T-shaped cells appearing at intervals, the “cuticle” forming a syncytial surface.Histochemical investigations indicate that the “cuticle” contains a combination of neutral and acid mucopolysaccharide, with a marked neutral predominance, the outer one displaying also an extremely thin coat of acid mucopolysaccharide with the sulfate group.The ordered arrangement of the microvilli suggests that this situation is imposed by the strong bond existing between the microvilli and the ouble mucopolysaccharide layers which would act as a cementing substance stabilizing the entire apical surface of the cell.
    Additional Material: 6 Ill.
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  • 36
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The length of the oviduct, the thickness of its wall, and the height of its mucosal epithelium and cilia were measured in (a) 0-, 2-, 4- and six-month-old rabbits, (b) rabbits ovariectomized at birth and (c) ovariectomized, estrogen-treated rabbits. The length and external diameter of the oviduct increased progressively until four months of age, after which their rates of increase declined. The thickness of the oviductal wall at the uterotubal junction was twice as large as that of the isthmus at two months of age and six times as large at four and six months of age. The height of the mucosal epithelium in the fimbriae was less than that in other oviductal segments at birth, but exceeded that in other segments at six months of age. Ciliated cells and motile cilia were absent 24 hours after birth; they were first observed two months after birth. The cilia of fimbriae were shorter than cilia elsewhere in the oviduct. Neonatal ovariectomy retarded the development of the oviduct and the mesotubarium and caused pyknosis of ciliated and non-ciliated cells of the oviductal mucosa. Cells with scarcely motile cilia were present five and one-half months after neonatal ovariectomy.
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  • 37
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 25-35 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The epidermis of 146 specimens of Dipsosaurus dorsalis and 182 Uma notata collected throughout the active period of the animals' year has been examined. The morphology of the epidermis is essentially similar to previously described lacertilians but differs in the relatively great degree of development of the mesos layer and the complete keratinization of the lacunar tissue prior to sloughing.Analysis of sloughing frequency throughout the year suggests that species specific patterns may exist, but these do not correlate with any particular known ecologic datum. The patterns do not reflect the reproductive activity of the two species supporting previous experimental conclusions on the lack of effect of gonadial hormones on epidermal activity.There appears to be no evidence of association of femoral gland activity with epidermal activity in D. dorsalis, but the situation is not clearcut in U. notata. These data are discussed in the light of recent studies of the evolutionary origin of epidermal glands in lizards.
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  • 38
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structural changes of mitochondria that occur during oogenesis and early embryogenesis in the rabbit have been examined with the electron microscope. Mitochondria of oogonia are both elongate and oval and contain a variable number of cristae which may or may not traverse the longitudinal axis of the organelle. When oogonia differentiate into oocytes, mitochondria become spheroidal and their cristae are sparse when compared with those found in the ellipsoidal organelles of concomitantly maturing follicle cells. As differentiation proceeds, the cristae of the mitochondria display varied configurations. For example, many display an arch-like arrangement in several regions of the organelle whereas others contain a pair of concentric membranes closely associated with limiting membrane of the mitochondrion.Mitochondria of stages from the fertilized egg to the morula possess the same internal structure as those of young oocytes. As the morula differentiates into a blastocyst there is a gradual increase in the size of the mitochondria and an increase in the number of cristae. We believe that the number and modifications of cristae indicate stages of mitochondriogenesis and the level of enzymatic activity in which this organelle is engaged during oogenesis and early embryogenesis.
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  • 39
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 129-135 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The reproductive tracts of several species of lizards were examined to understand the events assoclated with the transport of discharged oocytes following ovulation. At the onset of the reproductive cycle the regressed ovaries and oviducts appear as inconspicuous structures pressed against the dorsal body wall. As the reproductive cycle progresses, they undergo marked changes in position and size. The oviducts migrate ventrally and surround the growing ovarian oocytes, which move anteriorly where coelomic space is more abundant and eventually press intimately against the infundibulum. When ovulation occurs the ostium is in a remarkably strategic location for directly receiving the ovulated oocytes. The virtual isolation of the ovaries from the general coelom by the encapsulating oviducts, and the intimate association of the pre-ovulatory oocytes with the infundibular ostium do not support the current concept advocating the migration of loose ova following ovulation. An alternative explanation for the “trans-coelomic migratiion of ova” is proposed.
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  • 40
    Electronic Resource
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 41
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A fine structure study of spermatids and spermatozoa of the spider, Pisaurina sp. demonstrates that early spermiogenesis is similar to other flagellate spermatozoa. An acrosome forms from a Golgi-derived, acrosomal vesicle, a perforatorium indents acromosome and nucleus, a flagellum with a three-plus-nine tubule substructure is formed and nuclear chromatin condenses during spermiogenesis. Divergence from typical spermatozoa includes the presence of a three-tubule substructure of the central flagellar shaft, progressive rounding-up of late spermatids with concomitant incorporation of previously formed flagellum. This evidence is presented in terms of its possible functional significance in fertilization and gamete fusion in spiders.
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  • 42
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 1-15 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Gross morphology, staining characteristics and mapping of the diversity of the neurosecretory cell types in the brain and subesophageal ganglion of the scorpion Heterometrus swammerdami are reported. Special neurosecretory cell groups whose product is stainable with orange-G, acid fuchsin and Heidenhain's hematoxylin are present in the brain.In many of the living isolated neurosecretory cells, the secretory material appears luminous when viewed with dark ground illumination and granular when observed with phase contrast microscope.In the subesophageal ganglion the metameric arrangement of neurosecretory cells is distinct. Neurosecretory product accumulating in specific regions of subesophageal ganglion, and its axonal transport into the dorsal nerves and their termination in cephalic blood vessels apparently representing a storage and release organ of neurosecretion is reported.
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  • 43
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The placental memebranes and uterus of the garter snake, Thamnophis sirtalis, were studied using histological, histochemical, electron microscopic, dye transfer, and radioisotopic techniques. The conceptuses are completely enclosed throughout gestation by a transparent shell membrane which is produced by glandular epithelia in the uterine segment of the oviduct.Both chorio-allantoic and omphalo (yolk-sac) placentation are observed in this snake. The growth of the extra-embryonic mesoderm takes place in a manner peculiar to placental reptiles, and results in the isolation of the omphaloplacenta from the yolk-sac wall. On the basis of morphology, enzyme histochemistry, and phagocytosis of Trypan blue particles, this structure is interpreted as a site of histiotrophic absorption.The chorio-allantoic region of placentation is simple in structure. Fetal and maternal capillaries are closely apposed but always separated by layers of uterine and chorionic epithelium and the thin shell membrane. The placental membranes of the garter snake are similar in many respects to those of other live-bearing snakes, but less specialized than most lizard placentate.Isotopically labelled sodium and glycine are passed to the fetus following maternal injection, the latter at least apparently via the omphaloplacenta. The permeability to iron and phospate is extremely low. On the basis of these results and the selective transfer of certain dyestuffs, it appears that the shell membrane functions as a dialyzing membrane.
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  • 44
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 117-129 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Spermatozoa of the hydroid Pennaria tiarella were examined with the electron microscope. The anterior region is characterized by the presence of 30-40 membrane-bounded vesicles which lie anterior to the nucleus. These vesicles are apparently derived from the Golgi apparatus.The nucleus is conical in shape with a protrusion at the anterior end. Posteriorly it is indented by four radially arranged mitochondria. Lying within the fossa formed by the mitochondria are proximal and distal (filament forming) centrioles. The distal centriole is characterized by nine centriole satellite projections which emanate from its matrix. The tubules of the distal centriole are continuous with the alpha filaments of the tail.The tails are typical 9 + 2 flagella with 9 peripheral doublet (or alpha) filaments surrounding two central (or beta) filaments.
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  • 45
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The principal parenchymal elements of the submandibular glands of the heteromyid rodents Dipodomys merriami, Perognathus longimembris, Perognathus fallax, Perognathus penicillatus and Perognathus baileyi consist of acini, granular tubules and striated ducts.Acinar cells of the four species of Perognathus are aniline blue, PAS (magenta) and Alcian blue (pH 2.5) positive and metachromatic with toluidine blue and safranin. The granules of the tubule cells are orthochromatic and react with aniline blue, orange G, the PAS reagent (deep pink) and the tryptophan indicator, xanthydrol. Acinar and tubule cells of D. merriami exhibit similar reactions except for the Alcian blue stain. Acinar cells of D. merriami do not react with Alcian blue.Submandibular glands of D. merriami exhibit a sexual dimorphism of the granular tubules. There is little observable difference between the sexes in the species of Perognathus but the ratio of granular tubules to acinar elements, the degree of hypertrophy of the tubules, and the amount of mucosubstance and protein (granules) contained in their cells are different in the four species studied.Since these desert rodents have similar habitats and habits, the differences observed between the two heteromyid subfamilies studied, as well as among the four members of a single subfamily, suggest that these are inherent species variations rather than variations of adaptation to environment.
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  • 46
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The histology of the alimentary canal is described for the last instar larva, pupa, and adult Hyalophora cecropia (L.). Particular attention is given to the changes occurring in the midgut during the larval-pupal transformation and in the hindgut during the pupal-adult transformation. Descriptions are made as to the nature of the imaginal rings at each end of the midgut and a new structure is described in the larval ileum. The formation of adult rectal pads is followed in detail.
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  • 47
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 149-167 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The external prostate of male hedgehogs (Erinaceus europeus, Hemiechinus sp.) was studied at the beginning and at the end of the breeding seasons. Its tubular secretory units contain two types of epithelia, columnar and stratified. The columnar epithelium has abundant rough endoplasmic reticulum and forms secretory granules. The stratified epithelium undergoes cellular changes culminating in appearance of nuclear and cytoplasmic inclusions, nuclear pyknosis and desquamation of cells and nuclei into lumen. The nuclear inclusions contain protein and abundant silver-reducing substances. These inclusions may be derived from the residual protein of the nucleus.
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  • 48
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 169-179 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Integument from blue and green areas of the variant blue frog were analyzed biochemically for pteridines and carotenoids. Solvent extraction and absorption spectrophotometry indicated that β carotene was greatly reduced in the blue skin, and present in high quantities in the green skin of the blue frog. Thin layer and paper chromatography indicated that the pteridines were almost totally lacking in the blue skin, and present in normal quantities in the green skin of the blue frog.Light and electron microscopy indicated that the xanthophore pigment cells were either greatly altered or absent from the blue integument and present in the green integument. The fine structure of the xanthphores of the green integument contained the normal ultrastructural components of xanthopores found in regular green integument. The blue integument contained an abnormal cell type that occupied the position in the dermal chromatophore unit normally held by the xanthophores. The possibility of these cells being abnormal xanthophores or some other cell type is discussed.
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  • 49
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The spleen of the armadillo is invested by a capsule composed predominantly of smooth muscle. Inward extensions from the capsule contribute to an extensive system of trabeculae of the non-vascular type. The white pulp presents a typical follicular arrangement and the red pulp is permeated by an anastomosing series of venous sinsuses. The capillary segment of the penicillar artery is invested by an ellipsoid sheath. The spleen of the armadillo may be included in the class of mammalian spleens characterized as sinusal in nature and possessing ellipsoid sheaths. Electron micrographs of thin sections of the sheathed capillaries demonstrate a high-type of endothelium. A characteristic feature of this endothelium is the presence of a distinct intracytoplasmic fibrous component.The reticular cells comprising the sheath proper exhibit various sized vacuoles and inclusion bodies suggestive of their potentially phagocytic nature. In both light and electron microscopy, venous sinuses lie in close proximity to the peripheral cells of the ellipsoid sheaths. Within the wall of the venous sinuses, patent openings are appearent between the lining cells. If such an arrangement exists in the functional state of the organ, it could provide for an additional means of exposure of both plasma and cellular elements to the adjacent phagocytic cells of the sheath proper.
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  • 50
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 245-263 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The aggregation in vitro of embryonic neural retina cells was studied by electron microscopy with special emphasis on the reformation of intercellular junctions. The results show that (1) embryonic neural retina cells dissociated with trypsin retain morphological characteristics and polarity after dispersion into a suspension; (2) initial adhesions between the aggregating cells are nonspecific with respect to cell type and to the site of cell surface involved; (3) histogenetic associations in clusters of reaggregated cells appear within two hours after the start of aggregation. A hypothesis is presented that coated vesicles play a role in the formation of intercellular junctions.
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  • 51
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Observations on inner ear structure were made in five species of Scincidae, together with measurements of auditory sensitivity in terms of cochlear potentials. The basilar membrane and auditory papilla show a characteristic form, with considerable uniformity in dimensions except for a moderate expansion in the dorsal region and a more prominent one at the ventral end.A characteristic feature is the presence of a tectorial membrane that covers a large part of the surface of the medial limbus, but never leaves this surface and thus fails to make any contact with the auditory papilla. Hair-cell stimulation is achieved entirely through operation of the inertia principle (or equivalent principles) by means of a chain of sallets extending along most of the cochlea but giving way in the region of the ventral expansion to a single large body, the culmen papillae.The sensitivity varies in the five skinks studied from better than average to some-what below average in comparison with other lizard species. Thus an inertial (or inertia-like) system of hair-cell stimulation compares favorably with the tectorial membrane (restraint) system exhibited in the ears of most other lizards and all the higher animals.
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  • 52
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    Journal of Morphology 132 (1970), S. 313-333 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Spiders possess curved, blunt-tipped hairs on all legs and palps, which differ in many details from the straight, sharp-pointed, tactile hairs: (1) the blunt tip is open to the outside, which can be demonstrated by high resolution microscopy and by the penetration of dyes; (2) the hair shaft has a double lumen which consists of a circular (tube) and a crescent shaped lumen; (3) this hair is innervated by two to three bipolar neurons whose dendrites enter the small tube, where they arborize into 16-20 branches. Multiple innervation and an open tip give strong evidence for a chemoreceptive function. Concluding from their position and distribution on the distal leg parts, a contact chemoreception is tentatively proposed. This interpretation is supported by the close structural analogy to the known contact chemoreceptors in insects. Observation of behavior indicates the importance of a contact chemoreceptor on spider legs. Other possible chemoreceptors in spiders which have been described previously by other authors are discussed.
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  • 53
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In a study of survival of embryonic grafts in turtles, Chelydra was used as host and Chrysemys and Amyda as donors. Somites and overlying ectoderm with or without adjacent neural tube were transplanted. The operations were unilateral and orthotopic. The involved the anterior portion of the carapace. In other experiments, bilateral neural crest and dorsal neural tube were transplanted orthotopically.In experiments with Chrysemys as donor, pigment cells formed conspicuous red areas ventrally when neural crest was included in the graft. This pigment faded gradually but persisted for three or four years.When somites and adjacent ectoderm of Chrysemys carapace were transplanted, the graft area was lightly pigmented at hatching. This pigmentation increased subsequently. The Chrysemys grafts were either accepted or partially rejected. In cases of apparent complete acceptance, the graft region took on characteristics of the host.When Amyda served as donor of carapace rudiments, the graft area retained characteristics of the donor. At hatching, dark spots on a yellow background were present and scutes were absent. A few months after hatching, the graft area became necrotic. Subsequently, scutes with host characteristics or skin covered the graft area.
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  • 54
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    Journal of Morphology 132 (1970), S. 389-395 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Changing spermatozoan associations were observed in the epididymides of several mammals. These associations ranged from closely interwoven cylindrical bodies, found in the proximal part of the epididymis, to disorganized masses of spermatozoa, found in the distal part of the duct. It is suggested that changes in the cohesive properties of epididymal spermatozoa resulted in the formation and fragmentation of cylindrical bodies. These bodies, differeing in pattern and complexity according to the species, were found in all investigated mammals, including man. Cohesiveness appeared first in the upper part of the epididymidis, where it was confined to the spermatozoan tails. In general, there was a diminution of cohesive forces as the spermatozoa passed down the epididymal duct; consequently, the cylindrical bodies turned into disorganized masses of spermatozoa. There are indications that changes in the cohesive properties of spermatozoa may represent one aspect of spermatozoan maturation.
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  • 55
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 56
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 361-375 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Large number of annuli in Hirudinea are not true segments, and in the absence of spacious bodycavity and septa in adult no decision was taken regarding limit of a somite, until Gratiolet 1862 recognised a segment by colour marking, repetition of nephridial openings, and especially by the presence of segmental receptors, distinguishing first annulus of a segment. Whitman 1884 gave precision to these determinations and analyzed morphology of leeches to logical completeness. He recognised that though Hirudinaria and Hirudo have 102 body annuli and posterior sucker, true segments are only 26 plus 7.Castle ('00) and Moore ('00) proposed a new scheme of segmentation, with segmental receptor bearing annulus, as central annulus of a complete somite, with nerve ganglion, like that of other annelids, in center of a segment. They orientated everything roundabout the ganglion without noticing distorted fate of organ system.In this paper both the views are compared. Morphological and embryological studies reveal that the annulus bearing the segmental receptors in uniformly first annulus of all segments, including incomplete segments at the two extremities, with nerve ganglion in first annulus of the segment.Clitellum occupies three natural segments, IX, X, XI; crop caeca, nephridia, testis sacs, haemocoelomic channels and “rhomboidal figures” formed by ventrolaterals, all make a complete unit, well integrated in such segment. Conclusive evidence comes from the presence of septa at the level of each nerve ganglion in embryos of Hirudinaria. These observations corroborate Gratiolet and Whitman's view.
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  • 57
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 397-423 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Sperm from the male spermatophore in the red spotted newt are held indefinitely in convoluted, tubular outpocketings of the female cloaca called, collectively, a spermatheca. Each outpocketing, or tubule, when studied in thin section with the electron microscope showed a wall consisting of two components, a continuous inner or lining layer of epithelial cells and an outer covering layer of myoepithelial cells.The myoepithelial layer is penetrated by occasional openings or intercellular spaces through which the epithelial cells make contact with a basal lamina that bounds the tubule. The myoepithelial cells resemble smooth muscle cells displaying filaments (mean diameter = 75 Å ± 1 S.E.) that are probably constituted of actin, dense bodies and prominent caveolae. They are sparsely supplied with mitochondria, ribosomes, Golgi complexes and, sometimes, particles of glycogen. Typically the epithelial cells contain secretion granules, numerous mitochondria and ribosomes. They contain some fibers similar to those of the myoepithelial cells but with mean diameters that are significantly larger (90 Å ± 2 S.E.). Occasional profiles of Golgi complexes are evident and glycogen particles are abundant throughout the cytosomes of spermathecal tubules from adult animals bearing sperm. Secretory granules and glycogen are extremely rare or lacking in the spermathecae of efts that have not attained sexual maturity and in animals with ablated or involuted ovaries. In such animals large electron-lucid vesicles appear at or near luminal borders. Glycogen is absent and secretory granules show signs of dissolution in the spermathecal cells of gravid females induced to ovulate by the injection of chorionic gonadotropin. It is indicated that the epithelial cells provide nourishment for the sperm while they are retained within the spermathecal tubules, that the discharge of sperm from the spermatheca is facilitated by the contraction of the myoepithelial cells and that gonadal hormones act in the regulation of spermathecal function.
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  • 58
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A fine structural analysis of the ovotestis in Aplysia was undertaken in order to analyze the site of action of the bag cell hormone. Five stages of oocyte development are described. Of particular interest is the fact that the yolk seems to be synthesized primarily by the granular endoplasmic reticulum. In addition, small muscle cells whose long, thin processes surround the follicle of the ovotestis have been pointed out. This paper suggests that bag cell extract has a direct action on these small muscle cells causing them to contract and thus expel oocytes from the ovotestis. The evidence for this suggestion is that (1) these muscle cells are the most obvious effector cells in the ovotestis, (2) there are no signs of neural innervation of these muscles, (3) the time course for the liberation of the oocytes is so short that any other method of oocyte release is unlikely, (4) there is no cytologic evidence for any other expulsion process except muscular contraction, and (5) the ripe oocytes are attached to other cells of the wall of the ovotestis only by very small, simple junctions, thus making them the most likely cells to be expelled by muscular contraction.
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  • 59
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 1-1 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 60
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structure and polysaccharide constitution of the jelly capsule of the egg of Rana pipiens is described. Microscopic examination of the jelly capsule revealed the presence of five discrete jelly layers that differed clearly in their response to selected cytochemical tests. These layers were classified as M1-through M5 from the inner to the outermost layer. A sixth layer occasionally could be observed between M3 and M4.All layers contain neutral mucopolysaccharides. In addition layers M1 and M3 contain sulphated mucopolysaccharides, M2 and M4 contain non-sulphated acid mucopolysaccharides, and layer M5 contains both sulphated and non-sulphated acid mucopolysaccharides. M2 may also contain a small quantity of sulphated mucopolysaccharides. The layer that occasionally appears between M3 and M4 is probably an area in which free acidic groups are in higher concentration than in adjacent areas rather than being a discrete jelly layer. Neither hyaluronic acid nor sialic acid was localized by the methods employed.The possible significance of some of these constituents is discussed.
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  • 61
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Meiosis is described in virgin females, inseminated females and males of the acarid mite Caloglyphus mycophagus (Megnin). The observed sex determining mechanism is an XO-type with the male having a diploid chromosome number of 15. Oogenesis in mated females is regular. Pachytene is the earliest meiotic stage which is readily identifiable. At metaphase I eight bivalents are observed. Both products of the first maturation division divide at the second maturation division. After the fusion of the pronuclei either 15 or 16 chromosomes are observed in cleaving eggs.Nurse cells are not observed during the growth period of the oocyte. Such oocytes are attached to a central structure of the ovary by a cone-shaped organelle. At this stage the nucleus appears as a germinal vesicle; a nucleolus is present and the diffuse chromatin appears to extend from the nucleolus to the nuclear membrane. Nuclear extrusion bodies can be seen adjacent to the nuclear membrane both within and outside of the nucleus.Virgin females do not oviposit. The aberrant morphology and behavior of bivalents in post diakinetic oocytes which have not been penetrated by a sperm are described. Neither chromatin nor a chorion could be demonstrated in aberrant oocytes situated in the oviduct. It is suggested that oocyte degeneration in virgins is an adaptive feature in an animal order in which parthenogenesis is the more common mode of reproduction.
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  • 62
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It has been shown that the external parameters of eggs of the garden lizard, Calotes versicolor, are not suitable for assessing the exact developmental stages of embryos. In order to make use of this lizard's embryos for experimental work, a series of developmental stages has been characterized, using various morphological features.
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  • 63
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 467-478 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cytology of the vitellogenic stages in the development of the oocyte of Drosophila melanogaster is described following an electron microscopic study of sections of plastic-embedded ovaries and single egg chambers. One of the first morphological manifestations of yolk deposition is an infolding of the plasma membrane of the oocyte and the abscission of membranous tubules and vesicles. The protein (alpha) yolk spheres originate along the oocyte periphery from membranous sacs to which are attached membranous tubules. It is assumed that the majority of the protein within the alpha sphere is synthesized by neighboring tubular, rough surfaced endoplasmic reticulum. The other organelles in the ooplasm are described, and their origin and possible roles in vitellogenesis are examined. The relative importance of intra- and extra-ovarian synthesis of yolk protein in different insect species is discussed.
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  • 64
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In order to further support the thesis that myoblast fusions lead to the formation of multinucleate myotubes during early amphibian development, prospective myoblasts from frog embryos (Rana pipiens; Xenopus laevis) were orthotopically and heterotopically transplanted into embryonic salamanders (Ambystoma maculatum; A. tigrinum). The stages employed ranged from gastrula to early tail bud. After the hosts had developed to more advanced stages, they were fixed, sectioned in the frontal plane, and prepared for histological observation. Of special interest in these xenoplastic combinations are the nuclear and cytoplasmic differences between anuran and urodele embryonic cells.In most cases, transplanted cells tended to form a tissue complex distinct from the host tissues. This naturally decreased the opportunity for cell intermingling and consequently reduced the chances for fusions between graft and host myoblasts. Thus, there was a correlation between the extent of dispersion of the transplanted cells and the frequency of chimeric myotubes. Nevertheless, in each experimental series, a number of multinucleate myotubes emerged consisting of nuclear and cytoplasmic components of both graft and host cells. These chimeric myotubes, which must have originated by multiple cell fusions of anuran and urodele cells, (1) retained their distinct nuclear differences, (2) displayed resorption of both anuran and urodele yolk platelets, and (3) contained cross-striated myofibrils. The function of these chimeric cells, their synthesis of myofibrils, and the apparent lack of free intermingling at the cellular level, are discussed.
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  • 65
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: (1) The frontal wall of the encrusting cheilostome, Watersipora arcuata is a one-layered, evenly perforated calcareous shield which subdivides the metacoel into two compartments, the perigastric coelom and the hypostega. These compartments communicate through four and six “intrazoidal septulae” at the corners of the frontal wall. (2) The frontal wall develops by apposition independently of the ectocystal epitheca. The ascus develops as an invagination of the epitheca. These observations are consistent only with the view that the frontal wall of this species is a cryptocyst. (3) The terms “tremocyst,” “olocyst,” and “pleurocyst” are poorly understood and ambiguously defined; they should not be used until more information is available. (4) The taxa Anasca, Ascophora, Ascophora Imperfecta, and Ascophora Vera are polyphyletic and should ge dropped and replaced by Silén's (1942) more natural taxa. Silén's Cryptocystidea should be enlarged to include Watersipora and related ascusbearing cheilostomes.
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  • 66
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A mass of pineal tissue has been observed associated with the habenular commissure and the apex of the pineal recess in the golden hamster. Previously, it had been thought that all of the pineal system in the hamster was located at the confluence of sinuses just beneath the skull. To distinguish the two components of the pineal system, that in the dural position is called the superficial pineal and the mass associated with the habenular commissure is called the deep pineal.
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  • 67
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 68
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    Journal of Morphology 131 (1970), S. 195-209 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ovarian changes during the reproductive cycle of the oviparous garden lizard (Calotes versicolor) are described. It ovulates from last week of June to first week of September but most often in July and August when the monsoon occurs. The number of eggs ovulated vary from 10 to 32. After ovulation, the ovaries are reduced in size. From October to May, the ovaries contain small pre-vitellogenic follicles, which increase in size in June when most of yolk deposition occurs. Several nuclei are seen in the ooplasm of pre-vitellogenic follicles; they are finally absorbed before yolk deposition starts. Follicular atresia generally occurs in follicles with polymorphic granulosae, in post-ovulatory ovaries. Presumably interstitial gland cells are formed by the hypertrophy of the theca interna cells of atretic follicles. Pre-ovulatory follicles have highly vascularized thecae and invaginations of the follicular epithelium. After ovulation, the follicle cells hypertrophy to form the luteal cell mass filling the follicular cavity. Fibroblasts, which appear to arise from the theca interna, invade the luteal cell mass and form septa. Capillaries occur in the luteal cell mass.
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  • 69
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The adventitia of the crayfish heart consists of about five layers of uninucleated cells that often contain large amounts of glycogen. The outer and inner boundaries of the adventitia are represented by amorphous, homogeneous layers that are rich in neutral and acidic carbohydrates.The myocardium has a trabeculated appearance, due to the branching and anastomosing of the muscle cells. Studies on the localization of various oxidative enzymes show that the mitochondria are contained principally in the cell periphery and the myofibrils in the cell core. Intercalated discs appear as dark, irregular lines that traverse the muscle cell at a level that corresponds to that of a Z band. The myocardial cells branch and radiate from loci that are formed either by a single stellate cell or by the common end-to-end junctions (intercalated discs) of several cells. The function of these loci is discussed.The local cardiac nervous system consists of a Y-shaped ganglionic trunk that contains eight large anterior and eight small posterior nerve cells.
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  • 70
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Eleven different adult anuran species were observed for the presence or absence of the lymphomyeloid complex (LMI-7). The organs were compared with those of Rana catesbeiana, which were recently studied extensively. Three species (Kassina senegalensis, Bufo boreas and Bufo powerii) did not have any LM organs; six species, i.e., Hypopachus pearsei, Phrynomerus bifasciatus, Rana montezumae, Rana pipiens, Hyla arborea and Bufo marinus, had jugular bodies (LM3); Hyla had also procoracoid bodies (LM7); Bufo marinus had, in addition to LM3 and LM7, another pair that did not have any counterpart to those already reported. The two remaining species possessed entirely different sets of LM organs. Xenopus melleri had large, paired hemal nodes attached to the ventral surface of the laterorostral portion of the hyoid cartilage on either side of the midline and a lymphoid follicle in the neighboring connective tissue. The hemal nodes had a nodular cortex surrounding a nonnodular medulla; no lymphatic sinuses were found inside nor lymphatic vessels associated with these organs. Hemisus marmoratum had paired, small corpora subdermale that were partially overlapped by the depressor mandibulae. These were small, thickly encapsulated, well-vascularized lymphoid bodies. The organs in the six species were characterized as thinly encapsulated, highly vascularized, non-nodular lymphatic tissues; some were lymphomyeloid. The LM organs of starved Rana pipiens and Rana montezumae, in contrast to those of well-fed ones, were smaller and without fat cells. Lymphomyeloid organs of hibernating Rana catesbeiana were no different than those of non-hibernating frogs.
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  • 71
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    Journal of Morphology 131 (1970), S. 397-415 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Pygidial defence glands, which are universal in Caraboidea, are paired, cuticular invaginations of the body wall.In Pterostichus madidus F. the glands contain a nauseous, corrosive secretion that can be discharged forcibly to deter attack by predators. The cells producing the secretion are of one type only. Electronmicroscopy shows that the ducts which drain these cells are not intracellular but are separated from the cell contents by a plasma membrane. The cuticular walls of the duct appear to effectively screen the cell from the disruptive effect of its final products of secretion. The duct-carrying epidermal cells which are modifications of the epidermis covering the rest of the gland support the ducts from the secretory vesicles to their entry into the central lumen of each secretory lobe. There is a single duct-carrying cell around each duct.
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  • 72
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cyclic changes in the testis of the five-spined stickleback Eucalia inconstans (Kirtland) were studied histologically. Specimens were trapped between July 1965 and July 1967 in a shallow pond near London, Ontario.A three-dimensional microscopic study showed a main vas deferens and a system of primary, secondary and tertiary tubules.The testis cycle was divided into seven arbitrary stages. Spawning takes place from mid-April to mid-July. This is followed by the division of primary spermatogonia which are located along the walls of the tubules, producing cysts of spermatogonia enclosed in connective tissue which is surrounded by a thin epithelium. Both primary and secondary spermatocytes develop within these cysts. Breakdown of the cysts occurs with the development of spermatids and spermiogenesis occurs while spermatids are free in the tubules. Over-wintering of mature sperm takes place. Development of mature sperm from primary spermatogonia takes about 156 days.Germinal epithelium is absent but primary germ cells are believed to be those cells occupying the spaces between the tubules of the testis. No tissue which might be implicated in hormone production was observed.Phagocytic invasion of the testis has been studied. Massive infiltration by phagocytes is believed to be responsible for the sudden increase in testis weight observed during spawning. These cells ingest sperm nuclei and groups of them have been observed in the lumen of the tubules and the vas deferens, probably on their way out of the body.
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  • 73
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A correlation of autoradiographic and histochemical data indicates that the type I and II pulmonary epithelial cells are endodermally-derived; and, that the interstitial pulmonary cells are mesodermally-derived. Tritiated thymidine (T-H3) was found to be an excellent cell marker for in vivo developmental studies of mammalian (rat) lung. At a dose of 3 μc per gm (specific activity, 15.6-16.9 c per mM) maternal body weight, T-H3 crosses the placenta in amounts sufficient to effect heavy labeling of dividing cells. A partial placental barrier to T-H3 was found in late stages of development. Following an injection of T-H3 on day 16 of gestation, a higher rate of endodermal cell division was reflected by higher labeling indices and a steeper slope of the endodermal dilution curve as opposed to the mesoderm. This differential in labeling was maintained through the third postnatal day. Neonatal labeling patterns of the definitive cell types (type I and II pulmonary epithelial cells, interstitial pulmonary cells) reflected those of their germ layer precursors.Histochemical analysis of the developing rat lung demonstrated large accumulations of cytoplasmic glycogen in areas of rapid cell division (endodermal cells). As the mitotic rate decreased and cellular differentiation progressed, glycogen decreased; postnatally it is not a feature of mature pulmonary cell types.
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  • 74
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    Journal of Morphology 132 (1970), S. 47-67 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Study of a series of embryos showed that the spiroboloid leg arrangement (1 pair of legs on each of the first 5 segments) is derived from the typical leg arrangement (no legs on segment 1, 1 pair on segments 2 through 4, and 2 pairs on segment 5) by a shifting forward one segment of the first four pairs of legs.A careful re-examination of the literature, especially papers by Robinson ('07), Silvestri ('03, '49), Pflugfelder ('32), and Manton ('61), combined with observations of Narceus embryos led to the conclusion that (1) the anterior body segments are primatively single (2) the gnathochilarium is composed of only one pair of mouthparts, the diplopod head having but two gnathal segments, and (3) the intercalary segment is present in the Diplopoda.
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  • 75
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    Journal of Morphology 132 (1970), S. 101-107 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Several oxidative enzymes in the testis of the teiid lizard Cnemidophorus tigris were studied histochemically. The cells of the circumtesticular sheath (Leydig cell tunic) are functionally equivalent to Leydig cells of the interstitium on the basis of similar histochemical reactions for the five enzyme systems studied. Both groups of cells were positive for 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, NADH diaphorase, NADPH diaphorase, and glucose-6-phosphate dehydrogenase. These results support the hypothesis that the circumtesticular sheath has endocrine function as indicated by its vascularity and its ability to catalyze histochemical reactions involving steroid biosynthesis.
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  • 76
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    Journal of Morphology 132 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 77
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The organization of the corpora cardiaca and corpora allata of the female alfalfa weevil, Hypera postica, generally parallels that reported for other insects with extrinsic neurosecretory axons, intrinsic neurosecretory cells, and interstitial or glial cells appearing in both glands.Connecting the brain with the cardiacum are the extrinsic neurosecretory axons containing granules between 600 and 2200 Å. Granules with a diameter of up to 4000 Å are found in the intrinsic secretory cells of the corpus cardiacum.From the lobed corpus cardiacum, axons extend along a short nerve trunk into the ovoid corpus allatum. A characteristic of this gland is the presence of a layer of axons, ensheathing most of the corpus allatum and situated beneath an external basement membrane. These axons are believed to have their origin in the protocerebral neurosecretory cells. Although the c. allatum is known to be engaged in the release of hormonally active material, the cytoplasmic organization does not strongly reflect such activity.The glands of the diapausing weevil appear similar to those of the active weevil with a reduction in cytoplasm and a consequent folding of the plasma membrane.
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  • 78
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Somites, along with adjacent neural tube and overlying ectoderm, were extirpated unilaterally from embryos of Chelydra serpentina. Mesoderm of three somites was removed from various levels. The operations included the last formed somite and were done on embryos with 12 to 22 pairs of somites. In practice it was found that ventromedial portions of the somites were not included in the extirpation. The animals were preserved before pigmentation became heavy. The cartilaginous skeleton was stained selectively.The extirpations resulted in depletions of ribs consonant with relating the second rib to the fourteenth somite. The somites behaved as mosaics; they did not reconstitute each other nor did they regenerate after partial extirpation. The rudiments for the ribs were separable from the rudiments of the vertebrae, the sclerotomes, and were found to arise from a more lateral portion of the somite.The scutes are ectodermal derivatives, which are held to be dependent upon underlying somitic mesoderm for their differentiation. The extirpations resulted in abnormalities and depletions of scutes.
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  • 79
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    Journal of Morphology 132 (1970) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 80
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Virgin mosquitoes were studied with the electron microscope. Spermathecal duct walls contain cuticle, epithelium, and a richly innervated spiral muscle; myocytes are linked by desmosome-like attachment plaques to the underlying epithelium. Periductal cells along upper portions of the ducts have a large secretory droplet within a highly irregular extracellular lacuna and are attached to a long secretory ductule through which finely granular material is delivered to the duct lumen and this enters the spermathecae. Basal gland cells of spermathecae have short ductules containing secretion in virgins. Secretory material in spermathecae of virgins does not form a complete internal membrane.
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  • 81
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Neurosecretory cells of only one type (A, sub type A2) are seen in adult Melanoplus. Two groups of about 400 cells each are located dorsally in the pars intercerebralis medialis; four cells are located deep within the protocerebrum. We found no neurosecretory cells in other parts of the central or sympathetic nervous systems. In about 10% of the specimens, there was marked asymmetry in the location of the dorsal cell groups, with both of these groups and their axons located in one lobe of the protocerebrum.The nervi corporis cardiaci 1 cross-over in the corpus cardiacum, with the result that material produced by neurosecretory cells on one side of the brain is transported along axons that undergo two chiasmata to the corpus cardiacum of the same side.Stainable secretory material could be traced clearly from the cerebral cells to the corpus cardiacum, and even into the oesophageal nerves from the hypocerebral ganglion. However, stainable neurosecretory material is never present in the corpus allatum or along any of the nerves to this gland.
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  • 82
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    Journal of Morphology 131 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 83
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The integument and podia of the sea cucumber Thyone briareus were examined by bright field and electron microscopy. The epidermal surface was found to be covered by an acellular, PAS positive cuticle which appeared to be secreted by the underlying epidermal cells. Although the superficial portion of the cuticle contains numerous fine filaments, their ultrastructure bears no resemblance to collagen fibers. The epidermal cells are widely spaced and have long apical processes that extend along the under surface of the cuticle forming a contiguous epithelium. The apical expansions of the epidermal cells are attached to one another by means of septate desmosomes which may run continuously around all epidermal cells. Special attachment structures within these apical expansions appear to bind the cuticle to the dermis. The epidermal cells and their apical expansions are separated from the dermis by an 800 Å thick basement membrane. Granule containing cells in the upper dermis send processes up to the cuticle where they are bound to the typical epidermal cells by septate desmosomes. The abundant membrane bound granules of the cells enter villous-like processes which pass through the cuticle. The function of these cells may be to produce an adhesive material on the podia or they may be pigment cells.The thick dermis consists of a superficial zone, containing largely ground substance; a middle or laminated zone containing laminae of collagen fibers arranged in an orthogonal fashion; and a hypodermis consisting largely of ground substance and reticular fibers. Fibroblasts are abundant in the superficial dermis and between the collagen laminae. Wandering coelomocytes, or morula cells, accumulate between the collagen laminae and in the hypodermis. They may also become an integral part of the epidermis by forming septate desmosomes with epidermal cells. Morula cells contain highly specialized spherules whose tinctorial properties and electron microscopic appearance suggest that they contain protein and mucopolysaccharide.
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  • 84
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 85
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The present work describes the ultrastructure of the spinal cord in the regenerating tail of the lizard, Anolis. The distal growing region of the tail contains the advancing ependymal tube which is relatively devoid of axons but already contains channels between ependymal cell processes which anticipate their ingrowth. More proximally, fascicles of naked axons having their origin in the stump are present in the ependymal channels. Therefore, the pattern of fiber regeneration in the spinal cord is prescribed by the ependyma and not by the growing axons. Details of the ultrastructure of proximal, intermediate, and distal regions of the regenerate are reported. Particular attention is paid to the structure and differentiation of the ependymal cells and the relation of the ependyma to other glial cells, to nerve fibers, and to meningeal tissues.
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  • 86
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 253-275 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Submandibular glands of five adult female cats were examined by conventional electron microscopic techniques. All gland acini are mucous secreting and each acinus is capped with mucous secreting demilunar cells. Secretory product of demilunar cells is more electron lucent than that of acinar cells. The demilunes show intercellular tissue spaces and intercellular canaliculi whereas similar specializations are absent between acinar cells. Mitochondria and arrays of granular endoplasmic reticulum are more numerous in demilunar cells than in acinar cells. In acinar and demilunar cells secretory droplets first appear as enlarged Golgi saccules which subsequently become closely related to cisternae of the granular endoplasmic reticulum. Filamentous structures, interpreted as mucin molecules, are present in secretory droplets of acinar cells. Intercalated ducts are short, consisting of several junctional cells between acini and striated ducts. Striated ducts are long and tortuous and contain light cells, dark cells and basal cells. Light cells contain numerous membrane bound granules in their distal ends whereas dark cells show electron lucent vesicles in the same position. Basal cells contain a paucity of organelles and membrane plications but exhibit hemidesmosomes along their basal plasma membranes. Myoepithelial cells are abundant in relation to acinar and demilunar cells. Nerve terminals are present in some instances between acinar cells or between acinar and myoepithelial cells.
    Additional Material: 1 Ill.
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  • 87
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 359-382 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Small swellings near the base of the radial vein in each fore wing of the green lacewing, Chrysopa carnea, resemble typical insect tympanal organs, but some important differences are apparent. The swellings are bounded dorsally and laterally by thick cuticle and ventrally by thin, membranous cuticle. The ventral membrane is formed by a single, thin sheet of exocuticle with flattened hypodermis internally, but lacks the tracheal component that forms part of the tympanum in the typical insect tympanal organ. The portion of the membrane beneath each swelling is rippled while proximally it is smooth. In contrast to typical insect tympanal organs, the swellings in C. carnea are largely fluid-filled since an unexpanded trachea runs through each organ. A distal and a proximal chordotonal organ composed of typical chordotonal sensory units are associated with each swelling. The distal organ contains from five to seven units while the proximal organ is composed of from 18 to 20 units. Each sensory unit is composed of three readily identifiable cells. Distally, an attachment cell unites with the membrane and is contiguous with the scolopale cell, which surrounds the dendrite of the bipolar neuron. On the basis of the morphological evidence, one would not expect these swellings to function as sound receptors. However, the results of physiological and behavioral experiments, presented elsewhere, show that these organs are receptors for ultrasound.
    Additional Material: 1 Ill.
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  • 88
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 89
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Six types of sense organs are present on the antennal flagellum of Ctenolepisma lineata pilifera: tactile hairs, trichobothria, thick-walled chemoreceptors, small thin-walled chemoreceptors and coeloconic chemoreceptors. The number, size and distribution on the antenna of each type have been recorded. The base of the tactile hair is more complex than is that of other insects examined earlier. Trichobothria, long, slender hairs that oscillate in a gentle puff of air, are an unusal feature in insects and especially so for the antenna. The two types of thin-walled chemoreceptors differ in shape, size and in the structure of their walls and internal parts. A pocket-like depression of the floor of the cavity in which the peg of the coeloconic sense organ is set has not been found in earlier studies. Its function is unknown.The axons from the sensory neurons extend along the inner surface of the antennal epidermis as a sheet of fibers lining the antennal lumen. Near the pedicel the axons leave the epidermis and join to form the antennal nerve.A few observations on sense organs on appendages other than the antennae and some notes on behavior are included.
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  • 90
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 91
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In further consideration of the lizard ear, the fine structure of the cochlea has been investigated and related to auditory sensitivity in members of the family Cordylidae. The ear of this group of lizards is unusual in that a tectorial membrane is present only in a modified and seemingly vestigial form, and this membrane makes no connections with the auditory hair cells. These cells are provided instead with a series of sallets, small bodies extending in a single row through the dorsal and middle regions of the cochlea, where they rest upon the tips of the ciliary tufts and evidently bring about a stimulation of the hair cells because of their inertia. At the ventral end of the cochlea this line of sallets ends, and here is a single, relatively enormous structure, the culmen papillae, that serves a similar purpose for a large group of hair cells. Consideration is given to the manner of stimulation of the auditory sense cells in these species in relation to others with the usual arrangements involving connections between the ciliary tufts and a tectorial membrane.Included also is a study of a species of Gerrhosaurus, which some have included in the cordylid family and others have placed in a family of its own. The cochlear structure in this species is similar to that of the cordylids in many respects but differs in the ventral region, where instead of the culmen there is a heavy tectorial plate, similarly covering a large number of hair cells but connected to a tectorial membrane.The functioning of these ears is assessed in terms of the cochlear potentials, and is found to vary with species from better than average to excellent in comparison with other lizards investigated. The structural differentiation also is of fairly high degree, and hence it appears that ears without tectorial connections, or with such connections only in a limited region of the cochlea, can perform in a highly serviceable manner.
    Additional Material: 19 Ill.
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  • 92
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The black carpet beetle, Attagenus megatoma (Fab.), has been reported to exhibit negative phototaxis immediately after emergence. In later adult life, after the period during which most oviposition has occurred, the beetles are found to be photopositive. The compound eyes of one-day-old (Post-emergence) and nine-day-old (Post-ovipositional) female beetles were studied by electron microscopy and a number of strong differences were found between eyes at the two ages. The corneal facets of one-day eyes had the form of convex-concave lenses, while those of nine-day beetles were double-convex lenses. The primary and secondary pigment cells of young eyes were large and contained much endoplasmic reticulum and little accessory pigment. In the older eyes the pigment cells were reduced and contained much pigment, the proportion of endoplasmic reticulum being greatly reduced by comparison with the one-day eye. The cross-sectional area of the rhabdom was greater in the older eye.The possible relationships between age-related changes in eye morphology and behavioral changes during the same period are discussed.
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  • 93
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Desert-dwelling male Uma notata, U. inornata and U. scoparia were autopsied monthly from 1959 through 1962. Seasonal changes in the histological morphology of the testes were observed. A series of eight stages, indicating these changes in spermatogenic activity, was established. These categories arbitrarily divide a continuous process into several recognizable stages.Present evidence indicates that male breeding cycles can be determined rather precisely by examining any of several reproductive features. Data from several sources reveal that the male breeding season of U. scoparia is shorter than that of U. notata or U. inornata. Nevertheless, the testes of all three species appear to function reproductively during the hottest month of the year. Possible explanations for this ability are suggested.Little difference was observed in the time the seminiferous tubules of U. notata testes reached full breeding condition (stage 6) following dry winters. This is in contrast to the time motile sperm appeared at the cloacal end of the vas deferens in these animals. Thus is appears that the major cause of postponement in male U. notata reproductive activity following dry winters is due to a delay in sperm reaching the vas deferens rather than a retardation in sperm production.
    Additional Material: 5 Ill.
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  • 94
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 297-313 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Four types of haemocytes may be distinguished in Rhodnius adults, based on their fine structure. The plasmatocytes are most active in the repair of an integumental wound. They contain dens homogeneous granules whose contents become less dense in response to wounding until microtubules about 150å in diameter are distinguishable. The significance of these changes is not yet known. Tight and intermediate junctions, and septate desmosomes appear between haemocytes which have accumulated in an excision.
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  • 95
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 337-351 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of aging on the rat submandibular gland was studied by using ultrastructural, ultrastructural cytochemical and biochemical techniques. There was an age-related clumping of the nucleolar-associated and peripheral chromatin in many of the acinar cells and a decrease in the number of cisternae of rough endoplasmic reticulum. Many aged acinar cells were binucleated. There was also an age-related increase in pigment granules throughout the gland. These membrane bound granules consisted of a lipid droplet and an associated dense cap which had a granular matrix and pigment droplets. The lead capture method for acid phosphatase activity demonstrated that activity was associated with the granular matrix of the dense cap. These results were correlated with the age-related increase in acid phosphatase activity as determined by colorimetric procedures.There was an age-related increase in the number of cells characterized by small secretory granules. These cells were found as part of the intercalated ducts or at the junction of the duct with the acini. Oncocytes were also found as part of the parenchyma of the aged submandibular gland. These cells were characterized by the pleomorphic mitochondria that fill their cytoplasm. Occasionally, cells that possessed extraordinary numbers of mitochondria and small secretory granules were also observed.The determinations of total DNA and RNA revealed and age-related decrease in RNA while there was no significant change in the concentration of DNA.
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  • 96
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology of the cardiac ganglion of Limulus polyphemus (L) was examined by reconstructions from stained serial sections. This ganglion is composed of two distinct parts: a fiber tract extending the entire length of the heart and a cellular portion underlying the fiber tract. The cellular portion extends continuously from the third pair of ostia to the posterior terminus of the heart. The mean number of ganglion cell bodies is 231. Most of the ganglion cells are located among the glial elements of the cellular portion. The greatest density of cells is found in segments 5 and 6. Six cell types are recognized: (1) large pigmented unipolar cells approximately 120 μ in diameter with distinct connective tissue capsules around them; (2) large pigmented bipolar cells approximately 120 μ in length which are also encapsulated; (3) pigmented multipolar cells approximately 80 μ in diameter which are free of capsules; (4) small pigmented bipolar cells approximately 40 μ in length which are encapsulated but which are found exclusively within the fiber tract; (5) non-pigmented multipolar cells approximately 30 μ in diameter which are found scattered among the connective tissue elements of the cellular portion; and (6) small non-pigmented cells approximately 10 μ in diameter which are found within the unipolar cell capsule and scattered among the connective tissue elements of the ganglion.The variability in cell numbers and the random location of cells points toward non-specific anatomical connectivity between elements of this ganglion.
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  • 97
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Development of the secondary palate in Swiss white mouse embroyos was studied from age nine-and-one-half days in utero to the stage of mesenchymal coalescence in the secondary palate (approximately fifteen-and-one-half days). The greatest changes observed occur in the mesenchyme. At early stages, mesenchymal cells underlying oral ectoderm of the head are few and only occasionally contact the ectoderm. Electron micrographs show large intercellular spaces between the ectodermal cells. As embryogenesis continues, the mesenchymal cells become more numerous, closer to each other and closer to the epithelium. Just prior to horizontal transposition of shelves, the mesenchymal cells spread farther from each other and from the palatal epithelium and epithelium of the palatal tip becomes stretched. Ultrastructurally the intercellular spaces between epithelial cells of the palate tip have become much smaller. Some mitochondria in some epithelial cells are swollen and have clear matrices and distorted cristae. The shelves become horizontal and meet in the midpalate. Cells with degeneration bodies are seen in the epithelial seam. The seam undergoes autolysis and is replaced by mesenchyme. The morphological changes described, particularly in the mesenchyme, may play an important role in determining the effect of various teratogens at different stages of palatal development. The changes in both mesenchyme and epithelial cells in the later stages may constitute part of the process of preparing shelves for fusion as postulated by Pourtois ('66).
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  • 98
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 501-509 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Although there are many reports of tooth replacement patterns in lower vertebrates, few show the range of pattern to be found in a number of similar aged specimens of one species. Fifteen specimens of Caiman sclerops, head length 4-5 cms, were examined by a radiographic technique and their tooth replacement patterns analysed. Whole head radiography and dissected head radiographs were compared and the resulting tooth replacement waves were found to be comparable. Wave replacement (sensu Edmund, '60) in odd and even tooth positions in the tooth row was observed in all the specimens examined. Whereas most waves passed in a cephalad direction, wave reversal (caudad) was also observed, particularly in the anterior parts of the jaws. In some specimens simple alternation in tooth replacement was observed, particularly in the mid-portion of each quadrant. The smooth, age-related change-over from cephalad to caudad demonstrated by Edmund ('62) in captive Alligator mississippiensis was not observed in wild specimens of Caiman sclerops.
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  • 99
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 89-101 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Retrogressive analysis of the cn gene effect has been performed on crooked neck dwarf chick embryos between stages 28-38 (5-12 days). The phenocritical stage of mutant embryos studied is stage 29. Histolytic degeneration of neck tissues is first recognized by the appearance of localized degenerate nuclei in the tracheal mesenchyme. Pleiotropic autolysis of the embryonic thymus, loose mesenchyme and the ventral neck tissue is also observed. Histolysis occurs in a caudocephalic gradient in all cn-affected embryos. The degenerative effects in crooked neck dwarf embryos vary in their intensity, but the pattern of autolysis seems constant. Histological observations provide some explanation for “escapers,” homozygous lethal embryos known to survive until hatching. A mechanism for surviving developmental crises in cn embryos is proposed.
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  • 100
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 187-206 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A histological study of the spleen of the Brown-headed cowbird, Molothrus ater, is presented.One of the most striking differences from the mammalian spleen is the lack of trabeculae and of smooth muscle in the capsule which would suggest that the spleen is not an organ of storage or pumping of blood. Without trabeculae to foster the close association of the major arteries and veins, these vessels take separate courses. Their support is provided by elaboration of the collagenous and reticular fibres of the stroma.A peculiar ovoid structure, the ampulla, carries the blood from the terminal arterioles of the white pulp to both the sinusoids and the reticular cords of the red pulp so that both open and closed circulations are seen but the open circulation predominates. The ampulla has perforated walls consisting of a simple cuboidal endothelium surrounded by a dense reticular sleeve. Leucocytes were seen passing through the holes in the walls of the ampullae by diapedesis. It is suggested that the ampullae may be contractile and act as sphincters controlling the flow of blood through the spleen.The major functions of the spleen appear to be haemopoiesis, production of antibodies, and filtration of blood.
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