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  • 2000-2004  (3)
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  • Cat
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 130 (2000), S. 27-34 
    ISSN: 1432-1106
    Keywords: Key words Nucleus ambiguus ; Laryngeal motoneurons ; Superior laryngeal nerve ; Coughing and swallowing ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Membrane potential changes and discharges from 28 laryngeal motoneurons were recorded intracellularly in the caudal nucleus ambiguus of decerebrate, paralyzed and ventilated cats. Electrical activities were recorded from 17 expiratory laryngeal motoneurons (ELMs) with maximal depolarizing membrane potential in early expiration, and from 11 inspiratory laryngeal motoneurons (ILMs) with maximal depolarizing membrane potential in inspiration. Activities during breathing were compared with those observed during fictive coughing and swallowing evoked by electrical stimulation of the superior laryngeal nerves. These non-respiratory behaviors were evidenced in paralyzed animals by characteristic discharge patterns of the phrenic, abdominal nerves and pharyngeal branch of the vagus nerve. We recorded the activity of 11 ELMs and 5 ILMs during coughing in which ELMs, but not ILMs, exhibited increased membrane depolarization and discharge frequencies. Membrane depolarization and discharge frequencies of all ELMs were also significantly increased during swallowing. In addition, membrane depolarization of most ELMs (15/17) was preceded by a short-lasting hyperpolarization due to chloride-dependent inhibitory mechanisms occurring at the onset of swallowing. Out of 10 ILMs tested during swallowing, 7 exhibited membrane depolarization, preceded in 5 cases by a short-lasting hyperpolarization. Discharge frequencies of ILMs were significantly reduced during swallowing. The same pattern of phasic activities of ILMs and ELMs was observed during coughing and breathing, suggesting the involvement of similar excitatory pathways in both behaviors. These results imply that the duration of activation and the discharge frequency of neurons of the central generator for breathing that drive laryngeal motoneurons are enhanced during coughing. During swallowing, in addition to central excitatory mechanisms, laryngeal motoneurons are subjected to an initial inhibition of unknown origin. This inhibition probably contributes to the temporal organization of the swallowing motor sequence.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0603
    Keywords: Anto-immune disease ; Cat ; Dog ; Flow cytometry ; Immunodeficiency ; Leishmaniasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Clinical applications of flow cytometry to certain diseases of the dog and cat are now possible. The utility of such applications for diagnosis, prognosis and follow-up are illustrated here by a number of examples: feline AIDS resulting from FIV infection, Leukocyte Adhesion Deficiency in Irish setters, deep pyoderma in German shepherds, Immune-mediated Thrombocytopenia, canine Systemic Lupus Erythematosus and Leishmaniasis, Leukemia and Lymphoma.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0568
    Keywords: Key words Visual system ; Thalamocortical connections ; Postnatal development ; Retrograde axonal tracing ; Fluorescence technique ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The development of visual thalamocortical projections was analyzed quantitatively by comparing, in cresyl violet-stained brain sections of early postnatal (10–17 days) and adult cats, the cell body dimensions and total cell packing density (CPD) of neuronal populations in different laminae (A, A1 and C) of the dorsal lateral geniculate (dLGN), medial interlaminar nucleus (MIN), and in lateral (LPl), intermediate (LPi) and medial (LPm) subdivisions of the lateral posterior complex. Following injections of different fluorescent tracers (FB, NY, EB, RITC) into cortical visual areas 17/18, posterior medial (PMLS) and posterior lateral (PLLS) lateral suprasylvian and anterior ectosylvian (AEV), the thalamic distribution and densities of retrogradely labeled neurons were analyzed. Projection CPDs and ratios of projection/total CPDs were determined and compared within the different thalamic components in the kitten and adult cat. A significant decrease in total cell packing density was observed in the various thalamic components of the adult cat, varying between 43% and 65%, and a marked increase in mean cell body diameter in the A, A1 and C laminae and MIN from kitten to adult (8.4±1.8 and 11.8±2.8 µm respectively) compared to the LP subnuclei (9.0±1.3 and 9.1±1.5 µm). The ratios of projection/total CPDs decreased significantly for projections upon areas 17/18 stemming from layers A and A1 (20 and 25%, respectively) and from LPi upon both PMLS (34%) and AEV (16%). Thalamocortical projections observed in the kitten from LPi upon areas 17/18 and from the A-laminae upon PMLS were absent in the adult cat. The data indicate that, in comparison to the lateral posterior nucleus, the maturation of neurons within the dLGN and MIN is incomplete with respect to cell body size during the early postnatal period. In addition, the developmental changes observed involve both reductions in the total number of thalamic neurons and a differential loss of cortical projections. The selective elimination of early cortical connections stemming from dorsal lateral geniculate laminae A and A1 and from the intermediate division of the lateral posterior nucleus may occur through a process of axon collateral withdrawal from the expanded cortical sites, thereby giving rise to the adult pattern.
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  • 4
    ISSN: 1432-2072
    Keywords: Oro-facial dyskinesia ; Globus pallidus ; GABA ; Acetylcholine ; Behaviour ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The possible role of cholinergic mechanisms in the sub-commissural part of the globus pallidus (scGP) in the induction of oro-facial dyskinesia (OFD) was studied in cats. Local injections of the cholinergic agonist carbachol into the scGP elicited tongue protrusions in a dose dependent way (100–1000 ng/0.5 μl). The effect elicited by 1000 ng carbachol was selectively antagonized by the cholinergic antagonist scopolamine (10 μg/0.5 μl); this dose of scopolamine was ineffective when injected alone. The tongue protrusions resulted from both normal and abnormal movements: whereas normal movements simply consisted of protruding the flat tongue, abnormal movements implied a variety of movements, especially curling upwards the lateral side(s) or tip of the tongue inside or outside the oral cavity. The abnormal carbachol-induced tongue protrusions formed part of a syndrome marked by dyskinetic movements of the muscles of the eye, ear and cheek, and were identical to those seen previously after local injections of picrotoxin (250–500 ng). Intra-pallidal injections of the abovementioned dose of scopolamine had no effect on the tongue protrusions induced by local injections of 375 ng picrotoxin. However, local injections of 100 ng muscimol, which was previously found to attenuate significantly the effect of 375 ng picrotoxin and which was ineffective when injected alone, significantly attenuated the tongue protrusions induced by local injections of 1000 ng carbachol. These data suggest that the cholinergic effects are mediated via a GABAergic mechanism, but not vice versa. The results are discussed in view of GABAergic and anticholinergic therapies used in oro-facial dyskinesia.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 414 (1989), S. 235-244 
    ISSN: 1432-2013
    Keywords: Cat ; Urinary bladder ; Parasympathetic ganglion neurone ; Postganglionic stimulation ; Synaptic potentials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracellular recording techniques were used to examine and compare synaptic potentials evoked by stimulating pre- and postganglionic nerve trunks in cat bladder parasympathetic ganglia. In the 76 ganglion cells exammed, two types of responses were recorded on stimulating the postganglionic nerve: an antidromic action potential (type Post NS1;n=30) or a fast excitatory postsynaptic potential (f-EPSP; type PostNS2;n=46) which resulted in an orthodromic-like action potential. In some of the cells exhibiting a PostNS1 response (n=19), a fast depolarization was superimposed on the antidromic spike. This depolarization was due to the synaptic activation of nicotinic receptors. In many of the cells exhibiting either PostNS1 or PostNS2 responses, repetitive stimulation of the postganglionic nerve induced a slow hyperpolarization. Applying nicotinic (hexamethonium, methonium, 0.5–1 mM), muscarinic (atropine, 1 μM), alpha-adrenergic (phentolamine, 1 μM) and purinergic (caffeine, 0.5–1 mM) receptor antagonists completely inhibited the tetanus-induced slow hyperpolarization in some cells (n=5). In other cells (n=15), a slow hyperpolarization persisted in the presence of these antagonists. These results indicate that stimulation of the postganglionic nerve trunk of cat bladder parasympathetic ganglia can elicit not only an antidromic action potential, but also synaptic potentials which are mediated by the activation of cholinergic (nicotinic and muscarinic), noradrenergic and purinergic receptors, as well as a non-cholinergic, non-alpha-adrenergic and non-purinergic synaptic potential.
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  • 6
    ISSN: 1432-1912
    Keywords: 5-HT3 receptors ; Radioligand binding ; [3H]ICS 205-930 ; Cat ; Rabbit ; Vagus nerve ; Superior cervical ganglion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The binding characteristics of [3H]ICS 205-930, a 5-hydroxytryptamine 5-HT3 receptor antagonist, were investigated in membranes prepared from cat and rabbit vagus nerve (VN) and superior cervical ganglion (SCG). The autoradiographic localisation of 5-HT3 recognition sites was also assessed using [3H]ICS 205-930 in slices from cat medulla oblongata, nodose ganglion and vagus nerve. [3H]ICS 205-930 bound to a homogeneous population of high affinity recognition sites in cat VN: Bmax = 201 ± 43 fmol/mg protein, pKD = 9.26 ± 0.17 and SCG: Bmax = 291 ± 40 fmol/mg, pKD = 9.35 ± 0.80 (n = 3). Competition experiments performed in membranes from cat VN and SCG with agonists and antagonists suggested the presence of a homogeneous population of [3H]ICS 205-930 recognition sites. Competition curves were steep and monophasic and were best fitted by a 1 receptor site model. The following rank order of affinity for [3H]ICS 205-930 binding sites was observed with antagonists: SDZ 206-830 = ICS 205-930 〉 BRL 43694 〉 SDZ 206–792 〉 quipazine 〉 MDL 72222 〉 metoclopramide 〉 mCPP and agonists: 2-methyl-5-HT = 5-HT 〉 phenylbiguanide. A similar profile was observed for a limited series of compounds in rabbit membranes. Drugs acting at 5-HT1, 5-HT2 and dopamine receptors (domperidone, spiperone and metergoline) showed very low affinities for [3H]ICS 205-930 recognition sites. The sites labelled with [3H]ICS 205-930 in vagus nerve and superior cervical ganglion of both species displayed the pharmacological profile of a 5-HT3 receptor. There was a significant correlation between the rank order of affinity of the tested compounds for [3H]ICS 205-930 recognition sites in cat and rabbit membranes and their rank order of affinity for 5-HT3 receptors from neuroblastoma-glioma NG 108-15 cells. Autoradiographic studies suggest that [3H]ICS 205-930 binding sites are present over and around the nodose ganglion cell somata, along certain fibers of the vagus nerve and in the terminal areas of this nerve in the medullar nucleus of the vagus. The present data demonstrate that [3H]ICS 205-930 identifies 5-HT3 receptors in preparations of cat and rabbit vagus nerve and superior cervical ganglion.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 340 (1989), S. 764-766 
    ISSN: 1432-1912
    Keywords: Nucleus of the solitary tract (NTS) ; GABA release ; Carotid occlusion ; Blood pressure ; Push-pull cannula ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In anaesthetized cats, the nucleus of the solitary tract was bilaterally superfused through push-pull cannulae with artificial cerebrospinal fluid (CSF) and the effect of carotid occlusion on the release of endogenous GABA was investigated. Bilateral carotid occlusion led to a rise in blood pressure which was associated with a very pronounced increase in the release rate of GABA in the nucleus of the solitary tract. The results demonstrate the hypertensive function of GABA in the nucleus of the solitary tract and the importance of GABAergic neurons of this nucleus for the central cardiovascular control.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 79 (1989), S. 330-332 
    ISSN: 1432-0533
    Keywords: Sphingomyelinosis ; Neuropathology ; Histochemistry ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This report presents the clinical, morphological and biochemical findings on an 11-month-old female Siamese cat with neurovisceral sphingomyelinosis. Gross pathological features and histochemical findings are compared with the human disease counterpart and the previously described animal models. Hepatomegaly was observed while splenomegaly was not. Although sphingomyelin in liver and spleen was biochemically elevated, histochemical results in this case were slightly different from those previously recorded in human and feline Niemann-Pick disease. These results suggest that this feline case might be a different type of animal Niemann-Pick disease to that reported previously.
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  • 9
    ISSN: 1432-0568
    Keywords: Lateral reticular nucleus ; Cerebellar nuclei ; Retrograde transport of WGA-HRP ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cerebellar nuclear projection from the lateral reticular nucleus (NRL) was studied in 29 cats by means of retrograde axonal transport after implantation of the crystalline wheat germ agglutinin-horseradish peroxidase (WGA-HRP) complex in the cerebellar nuclei. It was confirmed that all the cerebellar nuclei receive afferent fibres from the NRL with the strongest termination in the ipsilateral interposed nuclei. In addition, these experiments give evidence of a previously unrecognized topical pattern in the projection to the interposed nuclei, arranged according to the same principle as in the projection to the immediately overlying cerebellar cortex. Thus, the anterior interposed nucleus receives fibres from all parts of the main NRL, its rostral part especially from laterally situated neurons, while subsequent more caudal parts from more medially situated neurons, while the posterior interposed nucleus receives fibres mainly from the dorsomedial part of the main NRL. The cerebellar nuclear projection to the NRL was investigated in 15 cats using retrograde transport after ventral microiontophoretical ejections of the WGA-HRP complex in the main NRL. The contralateral rostral fastigial nucleus was confirmed as the main origin of this projection, but projecting neurons were, in addition, discovered rostrally in the anterior interposed and dentate nuclei on the same side. No topical differences could be observed following ejections in different parts of the NRL; the majority of the projecting neurons were always concentrated along the ventral and lateral borders of the fastigial nucleus and in the adjacent medial part of the anterior interposed nucleus.
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  • 10
    ISSN: 1432-1106
    Keywords: Lateral geniculate nucleus ; Optic tract ; Sustained cells ; Transient cells ; Temporal luminance modulation ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We recorded the response of sustained (X) and transient (Y) cells in the cat lateral geniculate nucleus (LGN) and optic tract to a stationary spot while the spot luminance was increased and decreased with a constant rate (linear luminance functions), or modulated sinusoidally. The spot filled the receptive field center, and was surrounded by an annulus of fixed luminance. The LGN X cells seemed to perform a differentiation-like operation in the time domain at slow temporal modulations, giving information about rate of luminance change. To the linear luminance functions the cells responded with a constant firing rate. The on-center cells were activated during increasing luminance, the off-center cells during decreasing luminance. This firing rate increased monotonically with rate of luminance change. To low-frequency sinusoidal modulations the cells had a marked negative phase shift. The response of the LGN Y cells had a transient component shortly after the luminance started to increase (on-center cells) or decrease (off-center cells), followed by a secondary, gradually changing component. The peak of the transient component occurred on average when the response of the X cells increased most rapidly. To low-frequency sinusoidal modulation the average negative phase shift of this peak was twice the average of the X cells. The Y system could accordingly provide information about rate of change in the response of the X system. In the optic tract the X fiber response resembled the LGN X cell response in most respects. The Y fibers had only a weak transient response component, so this component was accentuated in the thalamic relay. Also the sensitivity for rate of luminance change was increased in LGN.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 74 (1989), S. 220-226 
    ISSN: 1432-1106
    Keywords: Cat ; Medial geniculate body ; Hearing ; Tonotopic organization ; Single unit recording
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The tonotopic organization observed in the present study for the pars lateralis (LV) of the medial geniculate body (MGB) in nitrous oxide anesthetized cats is generally consistent with that previously reported under barbiturate anesthesia. The present data, however, provide evidence for local deviations in characteristic frequency (CF) using appropriate sampling procedures of single units. Although the majority of pairs of units recorded simultaneously with the same microelectrode showed comparable CFs, a few pairs of such neighbouring units displayed CF disparities of up to 1.5 octaves. In addition, some units characterized by an elevated threshold had a CF deviating significantly from the general CF progression observed for the majority of units having low thresholds. This study points out the influence of the sampling procedure on the quality of the tonotopic organization observed in the MGB in addition to a possible effect of the level of anesthesia.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 75 (1989), S. 639-643 
    ISSN: 1432-1106
    Keywords: Motor cortex ; Somaesthetic responses ; Spinothalamic system ; Intracellular recording ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Evidence is presented that in the cat, the spinothalamic system contributes to short latency somaesthetic responses in motor cortex efferent cells. Intracellular recordings performed on identified pyramidal tract cells and corticospinal cells show that these cells are still activated and/or inhibited from the periphery after a set of central nervous lesions leaving intact only the ventral half of the spinal cord. The responses were attributed to the spinothalamic system. The ascending system is activated through collaterals of afferent fibres running in the dorsal columns of the spinal cord. This peripheral link to the motor cortex might participate in updating the motor command on the basis of information feedback from the periphery.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 76 (1989), S. 519-529 
    ISSN: 1432-1106
    Keywords: Pontine tegmentum ; Cholinergic neurons ; Single units ; Sleep-waking states ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A total of 260 neurons were recorded in the rostral pontine tegmentum of freely moving cats during the sleep-waking cycle. Of these, 207 neurons (80%) were located in the dorsal pontine tegmentum containing monoaminergic and choline acetyltransferase (ChAT)-immunoreactive, or cholinergic neurons. In addition to presumably monoaminergic PS-off cells (n = 51) showing a cessation of discharge during paradoxical sleep (PS) and presumably cholinergic PGO-on cells (n = 40) exhibiting a burst of discharge just prior to and during ponto-geniculo-occipital (PGO) waves, we observed tonic (n = 108) and phasic (n = 61) neurons exhibiting, respectively, tonic and phasic patterns of discharge during wakefulness and/or paradoxical sleep. Of 87 tonic cells histologically localized in the dorsal pontine tegmentum rich in cholinergic neurons, 46 cells (53%) were identified as giving rise to ascending projections either to the intralaminar thalamic complex (n = 26) or to the ventrolateral posterior hypothalamus (n = 13) or to both (n = 9). Two types of tonic neurons were distinguished: 1) tonic type I neurons (n = 28), showing a tonic pattern and high rates of discharge during both waking and paradoxical sleep as compaired with slow wave sleep; and 2) tonic type II neurons (n = 20), exhibiting a tonic pattern of discharge highly specific to the periods of paradoxical sleep. Tonic type I neurons were further divided into two subclasses on the basis of discharge rates during waking: a) rapid (Type I-R; n = 17); and b) slow (Type I-S; n = 11) units with a discharge frequency of more than 12 spikes/s or less than 5 spikes/s, respectively. Like monoaminergic PS-off and cholinergic PGO-on cells, both tonic type II and type I-S cells were characterized by a long spike duration (median: 3.3 and 3.5 ms), as well as by a slow conduction velocity (median: 1.8 and 1.7 m/s). In the light of these data, we discuss the possible cholinergic nature and functional significance of these ascending tonic neurons in the generation of neocortical electroencephalographic desynchronization occurring during waking and paradoxical sleep.
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  • 14
    ISSN: 1432-1106
    Keywords: Cutaneous EPSPs ; Fictive locomotion ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined modulation of transmission in short-latency, distal hindlimb cutaneous reflex pathways during fictive locomotion in 19 decerebrate cats. Fictive stepping was produced either by electrical stimulation of the mesencephalic locomotor region (MLR) or by administration of Nialamide and 1-DOPA to acutely spinalized animals. Postsynaptic potentials (PSPs) produced by electrical stimulation of low threshold afferents (〈 2.5 times threshold) in the superficial peroneal (SP), sural, saphenous or medial plantar nerves were recorded intracellularly from various extensor (n = 28) and flexor (n = 24) motoneurons and averaged throughout the step cycle, together with voltage responses to intrasomatic constant current pulses (in order to monitor relative cell input resistance). Each motoneuron studied displayed rhythmic background oscillations in membrane potential and correlated variations in input resistance. The average input resistance of extensor motoneurons was lowest during mid-flexion, when the cells were relatively hyperpolarized and silent. Conversely, average input resistance of flexor motoneurons was highest during mid-flexion, when they were depolarized and active. The amplitude of the minimum-latency excitatory components of PSPs produced by cutaneous nerve stimulation were measured from computer averaged records representing six subdivisions of the fictive step cycle. Oligosynaptic EPSP components were consistently modulated only in the superficial peroneal responses in flexor motoneurons, which exhibited enhanced amplitude during the flexion phase. With the other skin nerves tested (sural, saphenous, and plantar), no consistent patterns of modulation were observed during fictive locomotion. We conclude that transmission through some, but not all, oligosynaptic excitatory cutaneous pathways is enhanced by premotoneuronal mechanisms during the flexion phase of fictive stepping in several cat hindlimb motor nuclei. The present results suggest that the patterns of interaction between the locomotor central pattern generator and excitatory cutaneous reflex pathways depend on the source of afferent input and on the identity of the target motoneuron population.
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  • 15
    ISSN: 1432-1106
    Keywords: Pharyngeal vagus nerve ; Pharyngeal glossopharyngeal nerve ; Nucleus ambiguus ; Retrofacial nucleus ; Lateral reticular formation ; Nucleus of solitary tract ; Alaminar spinal trigeminal nucleus ; Horseradish peroxidase ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The central distributions of efferent and afferent components of the pharyngeal branches of the vagus (PH-X) and glossopharyngeal (PH-IX) nerves in the cat were studied by soaking their central cut ends in a horseradish peroxidase (HRP) solution. HRP-labelled PH-X neurones were distributed ipsilaterally in the rostral part of the nucleus ambiguus (NA) and the retrofacial nucleus (RFN); HRP-labelled PH-IX neurones were found in the ipsilateral RFN and the bulbopontine lateral reticular formation (RF). Vagal pharyngeal neurones constituted a large population of brainstem motoneurones. The population of HRP-labelled glossopharyngeal neurones was divided into two components. Indeed, on the basis of their location and somal morphology, the most ventral cells were identified as cranial motoneurones and those scattered in the lateral RF as parasympathetic preganglionic neurones. Application of HRP to the PH-IX nerve resulted also in the labelling of fibres and terminals in the alaminar spinal trigeminal nucleus and the nucleus of the solitary tract (NTS). The afferent fibres entered the lateral medulla with the glossopharyngeal roots, ran dorsomedially, then turned caudally toward the NTS and the caudal part of the alaminar spinal trigeminal motor (V) nucleus. In the NTS, labelled fibres ran mainly along the solitary tract, projecting to terminals in the dorsal and dorsolateral nuclei of the NTS.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 77 (1989), S. 94-102 
    ISSN: 1432-1106
    Keywords: Eye movement ; Brainstem ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study maps the eye movements evoked by microstimulations in the medulla of 9 alert cats. Trains of square waves (20 μA amplitude, 0.2 msec duration, 200 Hz) were delivered through glass-covered tungsten microelectrodes (0.5–1 MΩ). Movements of both eyes were recorded by the magnetic field/eye coil technique. Stimulation of the prepositus hypoglossi nucleus (PH) evoked nystagmus with ipsilaterally-directed slow phases followed by after-nystagmus with contralaterally-directed slow phases. Stimulation of the medial vestibular nucleus (MVN) induced nystagmus whose slow phases were more often ipsilaterally-directed but at time contralaterally-directed. At nearly all sites where we stimulated the reticular formation underlying prepositus and vestibular nuclei (from P4.5 to P12), we recorded versional conjugate movements. They were most often ipsilaterally-directed. Some microstimulations in the region of the medial longitudinal fasciculus evoked recentering eye movements: regardless of the initial position of the gaze (to the left as well as to the right), microstimulations given at the same place induced a movement of both eyes toward their neutral position. The amplitude of this movement was proportional to the eccentricity of the pre-stimulation position of the gaze.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 78 (1989), S. 336-344 
    ISSN: 1432-1106
    Keywords: Vagal pharyngeal nerve ; Glossopharyngeal nerve ; Single fibre recordings ; Respiratory-related units ; Superior laryngeal nerve ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In decerebrate, paralyzed and artificially ventilated cats, we recorded the discharge of 64 motor axons supplying the pharyngeal muscles. Filaments containing motor axons, with discharges related to the respiratory cycle (phrenic nerve activity), were teased from the pharyngeal branches of the vagus and glossopharyngeal nerves. Most units (n = 41) fired only during expiration and exhibited a steady, a decreasing or a late augmenting discharge pattern. These units were found only in vagal filaments. Twenty three units discharged during inspiration and exhibited a steady, a late augmenting or a tonic discharge pattern. The inspiratory-related units were present in both the vagus (n=13) and glossopharyngeal (n=10) nerves. Nineteen of 20 pharyngeal inspiratoryrelated units tested were activated at short latency (range 3.4 to 8.0 ms) by stimulation of afferents in the superior laryngeal nerve (SLN). In 13 of these, such stimulation also suppressed their spontaneous activity. SLN stimulation elicited in all 17 pharyngeal expiratory-related units tested a short latency (range 0 to 8 ms) reduction of activity, followed in 7 units by an increase in activity. SLN stimulation occasionally evoked single or rhythmic multifibre bursts in the vagal pharyngeal filaments. These bursts, involving expiratory-related units, likely correspond to the buccopharyngeal stage of swallowing.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 76 (1989), S. 182-186 
    ISSN: 1432-1106
    Keywords: Retinal ganglion cells ; Orientation bias ; Receptive field centre ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary For cat retinal ganglion cells whose receptive field centres were distributed in specified sections of the left visual field, the deviations of the major axis from the radial, horizontal, and circumferential directions were determined. The percentage of cells with deviations within ± 20° from the radial, horizontal, and circumferential directions were, respectively, 33%, 68%, 16%. In addition, comparison between values of deviation from the horizontal direction for cells located at eccentricities of 10° and 20° from the area centralis showed a statistically significant trend: the bias for the horizontal increased with eccentricity.
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  • 19
    ISSN: 1432-1106
    Keywords: Somatosensory cortex ; Directional sensitivity ; GABA inhibition ; Picrotoxin ; Bicuculline ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The role of intracortical inhibitory processes in the formation of neuronal receptive fields in the vibrissal projection zone of the somatosensory cortex was studied. Iontophoretic application of picrotoxin and bicuculline blocks the inhibition and causes the loss of directional sensitivity in neurons. Activation of inhibition by distant glutamate application gives opposite results — neurons become direction sensitive. A dependence was found between spatial location of activated cells and the pattern of changes of their detector properties. Inhibitory processes caused by natural afferent stimulation lead to similar changes in the functional properties of neurons.
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  • 20
    ISSN: 1432-1106
    Keywords: Bicuculline ; Inhibition ; Latency ; Receptive field profile ; Somatosensory cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In cortical area 3b of cats, responses of 76 single neurons to punctate indentations were recorded before and during iontophoretic administration of bicuculline methiodide (BMI), a GABAergic antagonist, at levels that did not affect spontaneous activity. Constant amplitude indentations were applied to selected sites along distalproximal and radial-ulnar axes that intersected the most sensitive area in the receptive field. Profiles of response magnitudes were used to measure receptive field dimensions before and during antagonism of GABAergic inhibition. Blockade of GABAergic transmission caused receptive field dimensions of 48 rapidly-adapting neurons to increase an average 141%, or nearly 2.5 times their original size. Analysis of the spatial distribution of inhibition indicated that in-field inhibition was larger than surround inhibition. During BMI administration, response latency was significantly longer for response elicited from the expanded territory than for responses elicited from within the original receptive field, suggesting that receptive field expansion might be mediated by multisynaptic intracortical connections. The magnitude of receptive field expansion was independent of receptive field size or peripheral location. In a substantial number of neurons, however, BMI produced asymmetric expansions that extended only in the proximal direction. For 9 slowly-adapting neurons, BMI produced measureable increases in receptive field dimensions, but these changes were significantly smaller than the changes in rapidly-adapting neurons.
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  • 21
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    Experimental brain research 77 (1989), S. 577-584 
    ISSN: 1432-1106
    Keywords: Motoneuron pool ; Inhibition ; Monosynaptic reflex ; Recruitment level ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The motoneurons to the Soleus muscle in the decerebrate cat were activated by the crossed extensor reflex, elicited by stimulation of the contralateral common peroneal (CP) nerve. Monosynaptic reflexes were obtained from the Soleus motoneuron pool by stimulation of the cut L7-S1 dorsal roots. The amplitude of the reflex increased approximately linearly with the recruitment level of the motoneuron pool. Tonic postsynaptic inhibition was induced in the Soleus moto-neuron pool by repetitive antidromic stimulation of the Lateral Gastrocnemius (LG) and Medial Gastrocnemius (MG) nerves at a rate of 17–47 stimuli/s. This reduced the size of the monosynaptic reflex at rest by at least 40%. However, when the motoneurons were active, the amplitude of the monosynaptic reflex obtained during repetitive stimulation of the LG-MG nerve increased with the recruitment level along the same curve as the control reflexes. Thus, tonic postsynaptic inhibition of the motoneurons per se cannot control the amplitude of the monosynaptic reflex independently of the recruitment level of the motoneuron pool. These experimental results verify predictions from computer simulations and suggest by exclusion that presynaptic inhibition is needed to control the amplitude of the monosynaptic reflex independently of the recruitment level of the motor pool.
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  • 22
    ISSN: 1432-1106
    Keywords: Control of respiration ; Intercostal muscle afferents ; Phrenic nerve ; Abdominal muscle afferents ; Expiratory neurons ; Abdominal muscle control ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Our objective was to determine if caudal ventral respiratory group (VRG) expiratory (E) neurons that drive abdominal expiratory motoneurons in the lumbar cord respond to intercostal and lumbar nerve afferent stimulation. Results showed that 92% of medullary E-neurons that were antidromically activated from the upper lumbar cord reduced their activity in response to stimulation of external and internal intercostal and lumbar nerve afferents. We conclude that afferent information from intercostal and abdominal muscle tendon organs has an inhibitory effect on caudal VRG E-neurons that drive abdominal expiratory motoneurons.
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  • 23
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    Experimental brain research 78 (1989), S. 374-379 
    ISSN: 1432-1106
    Keywords: Thermosensitivity ; Spinal cord ; Ascending pathways ; Behaviour ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The behavioural thermosensitivity of six cats was measured before and after single stage, symmetrical, bilateral, surgical lesions of the cervical spinal cord. The lesions were aimed at an area in the most ventral parts of the dorsal halves of the lateral funiculi. Unilateral lesions of that area have previously been found to cause reproducible, although subtotal, contralateral thermosensory defects, which were attributed to interruption of the thermosensory spinothalamic pathway. The lesions of three of the present cats were found to be incomplete, and those animals showed no postoperative thermosensory deficiency. Two of the cats with complete lesions showed marked post-operative defects, especially immediately after the operations, but the third cat with a complete lesion showed no postoperative thermosensory defect at all. The differences between the last three animals have been compared to the irregularity of previous reports about thermosensitivity after spinal cord lesions in man and animals, and may depend on the testing technique, rather than differences of thermosensitivity per se.
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  • 24
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    Experimental brain research 78 (1989), S. 501-513 
    ISSN: 1432-1106
    Keywords: Somatosensory cortex ; Interlaminar ; Corticortical ; SI ; Area 3b ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The morphology of single neurons in area 3b of cat primary somatosensory (SI) cortex was examined after horseradish peroxidase (HRP) injections. Neurons were labeled either by intracellular injection of HRP following intracellular recording or by small extracellular iontophoretic HRP injections. Both pyramidal and nonpyramidal neurons were labeled and reconstructed from serial sections. Their axons had local, interlaminar and interareal patterns of termination. Most neurons formed local axonal fields around their cell bodies and dendrites. Pyramidal neurons in cortical layer IV sent axons up into layers II and III, neurons in layers II and III sent axons down to layer V, and layer V neurons sent axons to layer VI as well as back to the upper layers. Layer VI neurons sent axons back to the upper cortical layers in a unique bowl-shaped pattern. The horizontal distribution of axons of pyramidal cells in layer III was extremely widespread. Axons of layer III neurons in area 3b terminated within 3b and area 1, but not in other areas of SI. Layer III neurons in area 1 distributed axon collaterals to all fields of SI as well as projecting a main axon to motor cortex. In general, the axon collaterals of area 3b pyramidal cells outside layer III remained confined to area 3b. Most of the nonpyramidal neurons labeled were basket cells in layers III and VI. These neurons formed dense axonal fields around their cell bodies, and none of their axons could be followed into the underlying white matter. The results of the present study demonstrate that area 3b somatosensory cortical neurons and their axons are vertically organized in a manner similar to that reported for other sensory cortical areas. They also show that widespread horizontal connections are formed by pyramidal neurons of layer III, and that these horizontal axons can travel for great distances in the cortical grey matter.
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  • 25
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    Experimental brain research 74 (1989), S. 131-138 
    ISSN: 1432-1106
    Keywords: Phrenic motoneurons ; Intracellular recording ; Interactions ; Recurrent EPSPs ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Intracellular recordings were made from 220 Phrenic Motoneurons (PM) in anaesthetized, spontaneously breathing cats, deafferented from C3 to C7, in order to look for somatic events related to the Recurrent Responses (RR) evoked in PM axons by repetitive stimulation of the phrenic nerve. RR appear sporadically at a constant latency, originate from a spinal nicotinic mechanism and can be evoked in a PM without the presence of an antidromic volley in its axon (Khatib et al. 1986). 2. Using stimuli effective for eliciting RR in axons, we failed to observe intracellularly somatic events corresponding to RR after the occurence of an antidromic action potential. RR were observed extracellularly in two cases, but in both cases the recording originated from axons. 3. We attempted to elicit somatic RR without a preceding antidromic action potential, using either parathreshold stimulation of the impaled PM, or suprathreshold stimulation of a phrenic strand which excluded the axon of the impaled PM. In both cases, RR-like events, with very stable latencies, appeared sporadically in 4/142 and 2/15 PMs respectively. 4. Parathreshold stimuli or stimulation of a strand were coupled with averaging of the synaptic noise in order to look for small events temporally related to the stimuli. Short latency small depolarizations, looking-like recurrent EPSPs, were revealed in 22/142 and 5/15 PMs respectively. 5. These results confirm the existence of interrelations between PMs, providing for re-excitation and coupling within the phrenic pool, in addition to centrally imposed synchronization.
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  • 26
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    Experimental brain research 74 (1989), S. 272-278 
    ISSN: 1432-1106
    Keywords: Cat ; Corpus callosum ; Optic chiasm ; Stereoacuity ; Visual acuity ; Visual fields
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We studied the role of the transcallosal pathway in stereopsis by measuring binocular and monocular depth perception in two cats that had undergone section of the optic chiasm at the age of 21 d. To ensure that the surgery did not impair vision to the extent that depth perception could not be evaluated, visual acuity and visual fields were also measured. In both of the chiasm-sectioned animals the visual fields were reduced and the visual acuity was substantially lower than in normal cats, with a maximum of about 2 cyc deg-1. Binocular depth thresholds of the chiasm-sectioned cats were worse than those of the normal cat but were better than their own monocular thresholds. These results suggest that the chiasm-sectioned animals were still able to use binocular cues to judge depth and indicate that the indirect pathway through the corpus callosum is sufficient to mediate binocular depth perception.
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  • 27
    ISSN: 1432-1106
    Keywords: Precerebellar nuclei ; Cerebellar cortex and nuclei ; Fluoro-Gold ; Rhodamine-B-isothiocyanate (RITC) ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The projections from certain brain stem precerebellar nuclei to the cerebellar cortex and nuclei have been examined in the cat by using the retrograde fluorescent double-labelling technique. Crystalline Fluoro-Gold was implanted into the left cerebellar nuclei from the contralateral side and rhodamine-B-isothiocyanate was injected into the overlying cerebellar cortex. The inferior olive, the lateral reticular nucleus, and the reticular tegmental pontine nucleus all contained double- as well as single-labelled neurons, and it was concluded that these nuclei have a high number of neurons whose axons branch to both the cerebellar cortex and nuclei. The neurons in the paramedian reticular nucleus and the pontine nuclei proper appear to project only to the cerebellar cortex.
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  • 28
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    Experimental brain research 75 (1989), S. 265-279 
    ISSN: 1432-1106
    Keywords: Rostral interstitial nucleus of the MLF ; Interstitial nucleus of Cajal ; Spinal cord ; Motoneurons ; Neck muscles ; Axial muscles ; Vertical eye and head movements ; Autoradiography ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eye and head movements are strongly interconnected, because they both play an important role in accurately determining the direction of the visual field. The rostral brainstem includes two areas which contain neurons that participate in the control of both movement and position of the head and eyes. These regions are the caudal third of Field H of Forel, including the rostral interstitial nucleus of the medial longitudinal fasciculus (riMLF) and the interstitial nucleus of Cajal with adjacent reticular formation (INC-RF). Lesions in the caudal Field H of Forel in monkey and man result in vertical gaze paralysis. Head tilt to the opposite side and inability to maintain vertical eye position follow lesions in the INC-RF in cat and monkey. Projections from these areas to extraocular motoneurons has previously been observed. We reported a study of the location of neurons in Field H of Forel and INC-RF that project to spinal cord in cat. The distribution of these fiber projections to the spinal cord are described. The results indicate that: 1. Unlike the neurons projecting to the extra-ocular muscle motoneurons, the major portion of the spinally projecting neurons are not located in the riMLF or INC proper but in adjacent areas, i.e. the ventral and lateral parts of the caudal third of the Field H of Forel and in the INCRF. A few neurons were also found in the nucleus of the posterior commissure and ventrally adjoining reticular formation. 2. Neurons in caudal Field H of Forel project, via the ventral part of the ventral funiculus, to the lateral part of the upper cervical ventral horn. This area includes the laterally located motoneuronal cell groups, innervating cleidomastoid, clavotrapezius and splenius motoneurons. At lower cervical levels labeled fibers are distributed to the medial part of the ventral horn. Projections from the caudal Field H of Forel to thoracic or more caudal spinal levels are sparse. 3. Neurons in the INC-RF, together with a few neurons in the area of the nucleus of the posterior commissure, project bilaterally to the medial part of the upper cervical ventral horn, via the dorsal part of the ventral funiculus. This area includes motoneurons innervating prevertebral flexor muscles and some of the motoneurons of the biventer cervicis and complexus muscles. Further caudally, labeled fibers are distributed to the medial part of the ventral horn (laminae VIII and adjoining VII) similar to the projections of Field H of Forel. A few INC-RF projections were observed to low thoracic and lumbosacral levels. It is argued that the neurons in the caudal Field H of Forel, which project to the spinal cord are especially involved in the control of those fast vertical head movements which occur in conjunction with saccadic eye movements. In contrast the INC-RF projections to the spinal cord are responsible for slower, smaller movements controlling the position of the head in the vertical plane.
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  • 29
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    Experimental brain research 76 (1989), S. 307-314 
    ISSN: 1432-1106
    Keywords: Moving stimuli ; Orientation specificity ; Preferred axes ; Spot-response-axis ; Striate cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The responses of 82 simple cells and 41 complex cells in area 17 of anesthetized and paralysed cats were examined with light bars of different length. For 84% of the simple cells and 66% of the complex cells the preferred axis of orientation of a stationary flashing long bar (orientational selectivity) and the preferred axis of movement of a small spot were parallel. As a consequence, the axis of maximal response to a moving light spot was mostly orthogonal to the optimal axis of a moving bar. Thus, a single cell responds to two perpendicular axes of preferred movement one for a long bar and one for a light spot, respectively. For both axes independent direction preferences could be distinguished. Additional preferred axes of movement between the two orthogonal extremes could be found with moving bars of intermediate lengths. This can be explained by the fact that cells with a pronounced response to a moving spot showed a strong tendency for intermediate bar length to elicit responses consisting of a superposition of both components. Therefore, decreasing bar length resulted in a gradual rotation of the preferred direction of movement from orthogonal to parallel with respect to the orientational axis, rather than to a mere widening of the tuning curve. Accordingly, the change in orientation selectivity with decreasing bar length is a regular transition from the orientation dependent response to a response type that depends only on the movement axis of the spot. Thus, in a simple model, the resulting response characteristic can be interpreted as an average of both components weighted according to the length of the stimulus.
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  • 30
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    Experimental brain research 77 (1989), S. 271-282 
    ISSN: 1432-1106
    Keywords: Red nucleus ; Motor initiation ; Single-unit activity ; Reaction time ; Delayed movement ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The activity of 98 Red Nucleus neurons was recorded in 3 cats operantly conditioned to perform a ballistic forelimb flexion movement triggered after a brief sound in a simple Reaction Time condition, or Delayed after the same sound in the presence of a tone cue. Fifty-eight task related neurons presented changes of activity in either one or both conditions. Forty-four of them were studied quantitatively and classified in 3 categories: 1) only 16% of the units presented similar changes of firing preceding the triggered or delayed movement; 2) most units (55%) presented different changes of activity in the two conditions: in the Delayed condition, the activation occurred earlier before the movement, and/or the change in magnitude was reduced or the pattern of activity was modified; 3) moreover, for 29% of the units, the change of activity observed before movement in the Reaction Time condition was severely reduced or even absent in the Delayed condition. For some of these neurons a building-up of activity was observed very early in the Reaction Time condition, during the preparatory period, well before the occurrence of the conditioned stimulus. These results show that the Red Nucleus activity preceding a movement is clearly dependent on its initiation conditions. The distinct patterns of unit firing observed in the Reaction Time condition and in the Delayed condition are tentatively related to the different preparation and initiation constraints determined by the behavioral conditions.
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  • 31
    ISSN: 1432-1106
    Keywords: NMDA receptor ; Visual cortex ; Excitatory amino acid ; Slice ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Actions of excitatory amino acid (EAA) antagonists on the responses of cells in layers II/III and IV of the cat's visual cortex to stimulation of layer VI and the underlying white matter were studied in slice preparations. Antagonists used were 2-amino-5-phosphonovalerate (APV), a selective antagonist for the N-methyl-D-aspartate (NMDA) type of EAA receptors, and kynurenate, a broadspectrum antagonist for the three types of EAA receptors. In extracellular recordings it was demonstrated that most of the layer II/III cells were sensitive to APV, while the great majority of the layer IV cells were not, By contrast, kynurenate suppressed the responses completely in both layers. Excitatory post-synaptic potentials (EPSPs) evoked by stimulation of layer VI and the while matter were recorded intracellularly from layer II/III neurons. To determine whether the EPSPs were elicited mono- or polysynaptically, the synaptic delay for each EPSP was calculated from a pair of onset latencies of EPSPs evoked by stimulation of the two sites. Forty-two percent of the layer II/III cells were classified as having monosynaptic EPSPs. In 60% of these monosynaptic cells, the rising slope of the EPSPs was reduced by APV while in the other 40%, it was not. In the former (APV-sensitive cells), subtraction of the APV-sensitive component from the total EPSP indicated that the onset latency of the NMDA receptor-mediated component was roughly equal to that of the non-NMDA component. In the latter (APV-resistant cells), only the slowly-decaying component was in part mediated by NMDA receptors. The conduction velocities of the afferent fibers innervating APV-resistant cells were slower than those of the APV-sensitive cells, suggesting that both types of cells are innervated by different types of afferents. The polysynaptic EPSPs of almost all layer II/III cells were sensitive to APV. The subtraction method indicated that the NMDA component had about the same magnitude as the non-NMDA components. When the slices were superfused by a Mg2+-free solution, the EPSPs were potentiated dramatically, but this potentiation was reduced to the control level during the administration of APV. Similarly, APV-sensitive components were potentiated during the administration of bicuculline, a selective antagonist for gamma-aminobutyric acid receptors of A type. These results suggest that NMDA receptors participate, at varying degrees, in excitatory synaptic transmission at most layer II/III cells in the cat's visual cortex, and their actions appear to be regulated by intracortical inhibition.
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  • 32
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    Experimental brain research 78 (1989), S. 203-213 
    ISSN: 1432-1106
    Keywords: Corpus callosum ; Development ; Vision ; Dark rearing ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It is now well established that during normal postnatal development there is a partial elimination of the callosal projections of cortical areas 17 and 18 in the cat and that visual experience early in life can modulate this process. In the present experiments, we quantitatively studied the influence of light, per se, by rearing cats in total darkness. Dark rearing exaggerates the normally occurring partial elimination of immature callosal projections: it causes a significant reduction in the total number of neurons in both the supra-and infragranular layers that send an axon through the corpus callosum and slightly narrows the distribution of these neurons across areas 17 and 18. These data demonstrate that visual stimulation is not necessary either to initiate the partial elimination of immature callosal projections or to stabilize a large fraction of the callosal projections present at birth. However, normal visual stimulation is necessary for the stabilization of the normal complement of callosal projections.
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  • 33
    ISSN: 1432-1106
    Keywords: Motor cortex ; Conditioned movement ; Posture ; Balance control ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The role of the sensorimotor cortex in the postural adjustments associated with conditioned paw lifting movements was investigated in the cat. Cats were trained to stand quietly on four strain gauge equipped platforms and to perform a lift-off movement with one forelimb when a conditioned tone was presented. The parameters recorded were the vertical forces exerted by the paws on each platform, the lateral and antero-posterior displacements of rods implanted on the T2, T12, L5 vertebrae as well as their rotation, and the EMG of triceps and biceps of both forelimbs. Before lesion, the postural adjustment consisted of a “nondiagonal” pattern where the CG was displaced laterally inside the triangle formed by the three remaining supporting limbs. Here a lateral bending of the thoracic column toward the supporting forelimb could be observed. The associated EMG pattern consisted of an early activation of the triceps lateral head in the moving limb which was probably responsible for the body displacement toward the opposite side, and a late biceps activation associated with the lift. In the supporting forelimb, a coactivation of the biceps and triceps was usually present. After contralateral sensorimotor lesion, the conditioned lifting movements were lost for 4–15 days after the lesion, before being subsequently recovered. The same lateral CG displacement and bending of the back was seen after lesion as before, which indicates that the goal of postural adjustment was preserved. However, the means of reaching it were modified. In most of the intact animals, the CG displacement was achieved in one step, whereas in the animals with lesions, the displacement was made either according to a slow ramp mode or in a discontinuous manner involving several steps. The mechanisms responsible for this disturbance are discussed.
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  • 34
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    Annals of hematology 58 (1989), S. 195-199 
    ISSN: 1432-0584
    Keywords: Cytochemistry ; Blood ; Bone marrow ; Leukocytes ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Blood and bone marrow cells of ten clinically healthy cats were stained for alkaline phosphatase (ALP), peroxidase (PO), chloroacetate esterase (CAE), alpha-naphthyl butyrate esterase (NBE), sudanophilia, and periodic acid-Schiff (PAS) reaction. Mature neutrophils in blood and bone marrow were devoid of ALP and NBE, but exhibited modest to strong PO, CAE, sudanophilia, and PAS reaction. In bone marrow, sudanophilia, PO, and CAE were prominent at the promyelocyte stage and diminished with cellular differentiation and maturation, while PAS reactivity increased with cell maturation usually from the myelocyte stage onwards. Myeloblasts were negative for all cytochemical reactions, but some large unidentifiable cells reacted strongly for ALP. Eosinophils were slightly reactive for ALP, CAE, and PAS, but not for PO, sudanophilia, and NBE. Basophil granules stained strongly for CAE, revealed PAS positivity, and stained negatively for PO, NBE, ALP, and sudanophilia. Slight ALP activity was detected in the intergranular cytoplasm of basophils. Lymphocytes and monocytes, with few exceptions, stained negatively. An occasional lymphocyte revealed slight globular NBE activity (NaF-resistant) and diffuse PAS reaction, while an occasional monocyte contained a few PO-positive and sudanophilic granules. Monocytes reacted modestly, whereas bone marrow macrophages reacted strongly for NBE (NaF-sensitive). Cells of the erythroid series stained negatively for all cytochemical reactions, megakaryocytes were PAS-positive, and platelets gave positive reactions for PAS and CAE.
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  • 35
    ISSN: 1573-0832
    Keywords: Sporotrichosis ; Sporothrix schenckii ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The isolation of Sporothrix schenckii from a female European cat it is described. The cat showed lengthened alopecic areas, with prominent nodules in the external surface of the thighs and abdomen. A mycological and histopathological studies of the lesions were carried out. The lesions resolved under treatment with 20% potassium iodide in doses of 0'1 ml/kg oral route in a 8 weeks period.
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  • 36
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    Cell & tissue research 258 (1989), S. 611-616 
    ISSN: 1432-0878
    Keywords: Cell communication ; Dye-coupling ; Odontoblasts ; Gap junctions ; Dentin sensitivity ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell communication between odontoblasts was investigated with the use of fluorescent-dye tracers; Lucifer Yellow CH (molecular weight = 457.3), and dextran-Lucifer Yellow CH (average molecular weight = 10000). Dyes were injected into cell bodies of individual odontoblasts via an intracellular microelectrode or into a group of cells through their processes, and passage to adjacent cells was examined with a fluorescence microscope. Lucifer Yellow CH appeared to diffuse very easily among odontoblasts, while dextran-Lucifer Yellow remained within the injected cell or cells. This efficient migration of Lucifer Yellow CH can be considered a functional manifestation of gap junctions between odontoblasts.
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  • 37
    ISSN: 1432-0878
    Keywords: Autonomic ganglia ; Spinal ganglia ; Sensory neurons ; Neurotransmitters ; Sweat glands ; Blood vessels ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neuronal subpopulations in the cat stellate, lower lumbar and sacral sympathetic ganglia were studied with regard to the cellular distribution of immunoreactivity to tyrosine hydroxylase (TH), acetylcholinesterase (AChE) and various neuronal peptides. Coexistence of neuropeptide Y (NPY)- and galanin (GAL)-like immunoreactivity (LI) was found in a high proportion of the neuronal cell bodies; these cells also contained immunoreactivity to TH, confirming their presumably noradrenergic nature. Some TH- and GAL-immunoreactive principal ganglion cells lacked NPY-LI. Two populations (scattered and clustered) of vasoactive intestinal polypeptide (VIP)- and peptide histidine isoleucine (PHI)-positive cell bodies were found in the sympathetic ganglia studied. The scattered VIP/PHI neurons also contained AChE-LI, calcitonin gene-related peptide (CGRP)-and, following culture, substance P (SP)-LI. The clustered type only contained AChE-LI. In the submandibular and sphenopalatine ganglia, neurons were AChE- and VIP/ PHI-immunoreactive but lacked CGRP- and SP-LI. Many GAL- and occasional TH-positive neurons were found in these ganglia. In the spinal ganglia, single NPY-immunoreactive sensory neuronal cells were observed, in addition to CGRP- and SP-positive neurons. The present results show that there are at least two populations of sympathetic cholinergic neurons in the cat. Retrograde tracing experiments indicate that the scattered type of cholinergic neurons contains four vasodilator peptides (VIP, PHI, CGRP, SP) and provides an important input to sweat glands, whereas the clustered type (containing VIP and PHI) mainly innervates blood vessels in muscles.
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  • 38
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    Cell & tissue research 257 (1989), S. 549-554 
    ISSN: 1432-0878
    Keywords: Salivary secretion ; Parotid gland ; Exocytosis ; Secretory granules ; Autonomic nerve-stimulation ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy of cat parotid glands revealed great heterogeneity in the secretory granules of normal unstimulated acinar cells. Electrical stimulation of the parasympathetic nerve to the gland evoked a copious flow of parotid saliva which was accompanied by an extensive depletion of the secretory granules from the acinar cells. Exocytosis was captured as it was occurring by means of perfusion-fixation, and showed that the events occur in a conventional manner. Stimulation of the sympathetic nerve caused only a very small flow of saliva, and no acinar degranulation was detected. It can be concluded that the parasympathetic secretomotor axons provide the main drive for parotid acinar degranulation in the cat. This contrasts with the rat in which sympathetic impulses provide the main stimulus for parotid acinar degranulation. These dissimilarities serve to emphasise how extensively species differences may influence autonomic responses in salivary glands.
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  • 39
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 1725-1729 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 40
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 1769-1785 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Methods are described for modelling of phase change processes using the finite element method to simulate freezing and melting including convection in the melt. Evaluation of several enthalply/specific heat methods and time marching schemes is also included. Suppression of velocities in the solid region is described, and example problems are given. Comparison is made to simulations performed by other researchers using finite difference methods. Substantially different results were found for one of these problems, and this result is shown to be caused by numerical problems in the earlier work.
    Additional Material: 14 Ill.
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  • 41
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 1813-1825 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The plane stress problem of a parallel fibre, rectangular composite sheet with variable fibre content is investigated. The resulting sheets are governed by partial differential equations that have variable coefficients, which are generally unsolvable exactly. A Ritz method is used to approximate the solution and is checked against known problems having exact solutions. The method is found to determine closely both the displacements and the stresses with fewer than 200 degrees of freedom. It is then applied to two interesting problems for which no exact solutions exist, including that of uniform normal stress applied in the direction of the fibres.
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  • 42
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1891-1908 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The paper addresses the question of the optimal selection of the shape functions for p-type finite elements and discusses the effectivity of the conjugate gradient and multilevel iteration method for solving the corresponding linear system.
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  • 43
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2466-2466 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 44
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    International Journal for Numerical Methods in Engineering 28 (1989) 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 45
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2517-2540 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The problem of static, non-linear, large three-dimensional deformation of riser bundles used in offshore oil and gas production is studied within the limits of small strain theory. The mathematical model consists of the models of component-risers and connectors which hold risers together. Each riser is modelled as a thin walled, slender, extensible or inextensible tubular beam-column. It is subject to non-linear three-dimensional deformation dependent hydrodynamic loads, torsion and distributed moments, varying axial tension, and internal and external fluid forces. The problem is solved numerically by developing an algorithm which features substructuring, condensation and non-linear incremental finite elements. Substructuring is used to decompose the riser bundle problem into those of individual component-risers and equilibria of connectors. Condensation is used along with the connector equilibrium equations to produce connector forces and moments. Strong non-linearities present in the model are handled by an incremental finite element approach. Accuracy of the computer code is verified by solving simple three-dimensional cases. Two three-dimensional applications are solved for a bundle with seven component-risers and up to a total of 1267 degrees of freedom. Finally, a comparison is made with numerical results of a two-dimensional analysis code. The influence of problem size on total CPU time is discussed.
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  • 46
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2609-2634 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The conceptual framework of a least squares rate variational approach to the formulation of continuously deforming mixed-variable finite element computational scheme for a single evolution equation was presented in Part I.1 In this paper (Part II), we extend these concepts and present an adaptively deforming mixed variable finite element method for solving general two-dimensional transport problems governed by a system of coupled non-linear partial differential evolution equations. In particular, we consider porous media problems that involve coupled heat and mass transport processes that yield steep continuous moving fronts, and abrupt, discontinuous, moving phase-change interfaces. In this method, the potentials, such as the temperature, pressure and species concentration, and the corresponding fluxes, are permitted to jump in value across the phase-change interfaces. The equations, and the jump conditions, governing the physical phenomena, which were specialized from a general multiphase, multiconstituent mixture theory, provided the basis for the development and implementation of a two-dimensional numerical simulator. This simulator can effectively resolve steep continuous fronts (i.e. shock capturing) without oscillations or numerical dispersion, and can accurately represent and track discontinuous fronts (i.e. shock fitting) through adaptive grid deformation and redistribution. The numerical implementation of this simulator and numerical examples that demonstrate the performance of the computational method are presented in Part III2 of this paper.
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  • 47
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2161-2174 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: An a-posteriori error estimator for finite element analysis proposed by Zienkiewicz and Zhu is analysed and shown to be effective and convergent. In addition we analyse wider classes of estimators of which the Zienkiewicz-Zhu estimator is a special case. It is shown that some of these estimators will be asymptotically exact. Numerical evidence is presented supporting the analysis.
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  • 48
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2175-2190 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In the case of nearly incompressible elastic materials the strain energy, the shear stress and the difference of normal stresses can be computed accurately by direct methods when the p-version of the finite element method is used. Computation of the sum of the normal stresses requires special procedures. In this paper such procedures are described and examples are presented.
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  • 49
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2123-2142 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Considerable progress has been made in recent years toward advancing the state-of-the-art in solid mechanics boundary element technology. In the present work, much of this new technology is applied in the development of a general-purpose boundary element method (BEM) for steady-state heat conduction. In particular, the BEM implementation involves the use of higher-order conforming elements, self-adaptive integration and multi-region capability. Two- and three-dimensional, as well as axisymmetric analyses, are incorporated within a unified framework. In addition, techniques are introduced for the calculation of boundary flux, and for the inclusion of thermal resistance across interfaces. As a final extension, an efficient formulation is developed for the analysis of solid three-dimensional bodies with embedded holes. For this last class of problems, the new BEM formulation is particularly attractive, since use of the alternatives (i.e. finite element or finite difference methods) is not practical. A number of detailed examples illustrate the suitability and robustness of the present approach for steady-state heat conduction.
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  • 50
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    International Journal for Numerical Methods in Engineering 28 (1989) 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 51
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2191-2202 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This note reports numerical experiments on the efficiency of simple error estimates derived earlier1 applied to incompressible mixed or related penalty type formulations. The rate of convergence and performance of various mixed elements is compared. Numerical results from a driven cavity and an incompressible elastic problem demonstrate that the T6B1/3D and T6/3C elements give a faster rate of convergence than the T6/1D element. However, in the case of a plane extrusion analysis (stronger singularity), the rate of convergence for the T6B1/3D element drops and is inferior to that of the T6/1D, while the T6/3C element still proves superior to the other two elements.
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  • 52
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2839-2853 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The Zienkiewicz-Zhu error estimator is shown to be effective in problems of plate flexure. When used in conjunction with triangular elements and an adaptive mesh generator allowing a prescribed size of elements to be developed, very fast adaptive convergence for results of specified accuracy is achieved.
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  • 53
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2889-2906 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The construction of changing sequences of irregular and nested triangulations, based on the use of conforming refinement/derefinement algorithms for triangulations, is presented and discussed. This strategy is particularly appropriate to combine adaptivity and full multigrid algorithms for dealing with moving fronts or fluid dynamics problems. It is shown that the quality of all the triangulations iteratively generated depends only on the geometric characteristics of the initial grid. A data structure suitable to create, manage and modify series of nested triangulations as well as the main features of the DEREF prototype package are described, and numerical examples are given.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2923-2941 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A unified presentation of some popular continuation procedures used in the non-linear finite element analysis of structural mechanics is introduced. An extension of the elliptical constraint equation proposed by Crisfield is given. It is shown that in the proposed procedure real roots can always be obtained in solving the iterative change of the load parameter. Updated weighting factors are introduced in the constraint equation in order to get better convergence characteristics in the case when localized deformations occur. For bifurcation points a modification of Rheinboldt's branching procedure is presented. Post critical response after limit and bifurcation points is determined in some numerical examples.
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  • 55
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 21-35 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A numerical model system is developed to describe waves and wave-induced currents in a breakwater situation, where refraction, diffraction, reflection and wave-current interaction may all play important roles in the transformation of waves. A turbulence transport model is employed to estimate the eddy viscosity, and the friction factors associated with the major variables are clearly defined using a high level closure of combined flow bed friction. The model system is tested against a laboratory situation, and the effects of reflection are demonstrated on the wave-induced currents as well as on the wave distribution.
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  • 56
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 3-20 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: During the past ten years various methods have been devised to deal with the solution of the Navier-Stokes equations. In the meantime, different models have been worked out to represent turbulent incompressible flows. At LNH, deep participation in this enthusiastic research is still going on, together with a closely related development of physical modelling and metrology. In this paper, we will give an insight into a set of numerical codes which are now currently in use at our laboratory and some of their main applications to coastal engineering. This will include thermohydraulic internal flows and free surface flows. For each code a short presentation of the algorithm of solution is described.
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  • 57
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 71-85 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A study of linear monochromatic wave propagation is presented, based on a theory of potentials. The usual Berkhoff equation has been extended by the addition of partially pervious solid boundary effects and the bed friction.The problem has been solved by a hybrid finite element method including the effect of singular local elements such as the end of breakwaters or wharfs. This numerical method confirms the interest in quadratic Lagrangian finite elements.The comparisons with analytical solutions and existing numerical results show the validity of the method. Some original examples also prove the good performance of the proposed computation method.As an example, the study of the erosion of the beach of Calvi Bay in Corsica, France is presented. The phenomenon had started already in 1960, but the problem began to be very severe four years ago, after the implementation of a new harbour in 1982 and buildings, hotels, restaurants, and a main sewer on the beach. To consider the impact of these constructions and other factors like waves, currents and geomorphological aspects, the studies were divided into four parts: sedimentological, currents, wave propagation and geomorphological.This paper will describe the results of a complete two years study on the site, from February 1986 to December 1987.Findings show that the erosion process is due to the rise of the relative sea level with, as a catalyst, tourist behaviour and the implementation of the new constructions.
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  • 58
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 469-481 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Piecewise linear finite element approximations to two-dimensional Poisson problems are treated. For simplicity, consideration is restricted to problems having Dirichlet boundary conditions and defined on rectangular domains Ω which are partitioned by a uniform triangular mesh. It is also required that the solutions u ∊ H3 (Ω). A method is proposed for recovering the gradients of the finite element approximations to a root mean square accuracy of O(h2), both at element edge mid-points and element vertices, using simple averaging schemes over adjacent elements. Piecewise linear interpolants (respectively discontinuous and continuous) are then fitted to these recovered gradients, and are shown to be O(h2) estimates for ∇u in the L2-norm, and thus superconvergent. A discussion is given of the extension of the results to problems with more general region and mesh geometries, boundary conditions and with solutions of lower regularity, and also to other second-order elliptic boundary value problems, e.g. the problem of planar linear elasticity.
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  • 59
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 109-126 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: This paper presents the development of a straightforward displacement type triangular finite element for bending of a flat plate with the inclusion of transverse (or lateral) shear effects. The element has twenty two degrees of freedom consisting of ten for the lateral displacement of the midplane and six for rotations of the normal to the undeformed midplane of the plate. The latter are taken as independent of the slopes of the deformed midplane in order to include deformation due to transverse shear. The element is fully conforming and may be orthotropic. At interelement boundaries, the element matches adjacent elements both with respect to lateral displacement of the midplane and the rotations of the normal. The result is an efficient ‘linear moment’ triangular element but with transverse shear deformation included. Numerical computations for a number of examples are presented. The results show the element to be more flexible than most other finite element models and agree closely with those from a numerical solution of the three dimensional elasticity equations. The results also converge to those from thin plate theory when the thickness to length ratio becomes small or when the transverse shear moduli are artificially increased.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 237-237 
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    Keywords: Engineering ; Engineering General
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 279-293 
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    Notes: An iterative solution procedure for a frictionless contact problem is presented. Convergence to the exact solution is guaranteed by an error reduction method, and the rate of convergence is drastically improved by reduction of the ratio of extreme eigenvalues of the iteration matrix. For an ordinary linear equation, the present technique has the same theoretical value of convergence rate as the Chebyshev acceleration technique. The present acceleration technique can be directly extended for complicated frictional contact problems.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 415-430 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Moving finite element methods are adaptive gridding procedures especially designed for systems of partial differential equations whose solutions contain steep gradients. A new moving finite element method based on quadratic approximation functions is presented. Both the theoretical and computational aspects are outlined. Performance of the method is illustrated with solutions to Burgers' equation. The solution is accurate and remarkably smooth in the entire domain.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1461-1473 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: An enriched finite element model for linear elastic fracture mechanics is developed for a mixed variational statement. The independent approximations for the displacement and stress components are enriched by adding the near-field analytic expressions for a cracked body to the polynomial approximations of a conventional element. This allows for an accurate representation of the stress and displacement fields near the crack tip and also results in the direct calculation of the appropriate stress intensity factors. The accuracy of this formulation is demonstrated through several numerical examples.
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    International Journal for Numerical Methods in Engineering 28 (1989) 
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1521-1537 
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    Keywords: Engineering ; Engineering General
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    Notes: The nature of the crack and the structure behaviour can range from ductile to brittle, depending on material properties, structure geometry, loading condition and external constraints. The influence of variation in fracture toughness, tensile strength and geometrical size scale is investigated on the basis of the π-theorem of dimensional analysis. Strength and toughness present in fact different physical dimensions and any consistent fracture criterion must describe energy dissipation per unit of volume and per unit of crack area respectively. A cohesive crack model is proposed aiming at describing the size effects of fracture mechanics, i.e. the transition from ductile to brittle structure behaviour by increasing the size scale and keeping the geometrical shape unchanged.For extremely brittle cases (e.g. initially uncracked specimens, large and/or slender structures, low fracture toughness, high tensile strength, etc.) a snap-back instability in the equilibrium path occurs and the load-deflection softening branch assumes a positive slope. Both load and deflection must decrease to obtain a slow and controlled crack propagation (whereas in normal softening only the load must decrease). If the loading process is deflection-controlled, the loading capacity presents a discontinuity with a negative jump. It is proved that such a catastrophic event tends to reproduce the classical LEFM-instability (KI = KIC) for small fracture toughnesses and/or for large structure sizes. In these cases, neither the plastic zone develops nor slow crack growth occurs before unstable crack propagation.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2113-2122 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Finite element partitioning (or substructuring) is employed to estimate the eigenproperties of large-scale structural systems. A homotopy equation is constructed and its solutions are characterized by a number of curves which connect the eigensolutions of the partitions with those of the complete system. A step-by-step tracing procedure is developed to follow these curves. At each step, prediction and correction are performed. The Rayleigh-Ritz procedure and the conjugate gradient method are used as predictor and corrector, respectively. Compared with the sole use of either the Rayleigh-Ritz or gradient methods, the proposed method is more reliable and more efficient for large-scale problems. Numerical implementation is well suited for supercomputers.
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  • 67
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    Notes: A finite element formulation has been developed to take into account the warping effect of composite beams undergoing large deflection or finite rotation. This formulation is to be used to model combined bending, torsional and extensional behaviour of composite helicopter rotor blades. The new approach can model thin to moderately thick walled composite beams with complicated cross-sections, tapers and arbitrary planforms. The warping effects are incorporated by assuming warping displacements superimposed over cross-sections normal to the beam axis in the deformed configuration of a shear flexible beam. The strain is assumed to vary linearly through the wall thickness. The fixed or total Lagrangian description is adopted in the present formulation and the Newton-Raphson method is used to solve the non-linear equilibrium equation resulting from the finite element approximation. Numerical tests of example problems demonstrate the validity and effectiveness of the present approach.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2275-2292 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A plate bending element based on the generalized laminate plate theory (GLPT) developed by the senior author is described and its accuracy is investigated by comparison with the exact solutions of the generalized plate theory and the 3D-elasticity theory. The element accounts for transverse shear deformation and layer-wise description of the inplane displacements of the laminate. The element has improved description of the inplane as well as the transverse deformation response. A method for the computation of interlaminar (transverse) stresses is also presented.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2323-2338 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Application of a weak form of the Kirchhoff constraints in the formulation of triangular assumed shear strain C0 plate element is described. It is shown that, compared to a similar formulation of the quadrilateral elements, formulation of the triangles requires some additional considerations which lead to additional terms in the energy expression. With those terms performance of the assumed shear strain triangular elements is comparable to that of the quadrilateral elements.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2397-2421 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Robinson's viscoplastic model, a representative of the so-called overstress models, is integrated by use of the generalized midpoint rule. The solution of the non-linear system of algebraic equations arising from time discretization of the constitutive equations is determined using a projection method in combination with Newton's method. Consistent tangent moduli are calculated and the quadratic convergence of the global Newton equilibrium iteration is shown. The time increment size is controlled by the convergence behaviour of the equilibrium iteration and the accuracy of the numerical integration. Various numerical examples are considered to demonstrate the efficiency of the methods.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2711-2711 
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2761-2776 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The performance of group implicit algorithms is assessed on actual concurrent computers. We show that, as the number of subdomains is increased, performance enhancements are derived from two sources: the increased parallelism in the computations; and a reduction in equation solving effort. Moreover, we show that these two performance enhancements are synergistic, in the sense that the corresponding speed-ups are multiplied, rather than merely added. Our numerical simulations demonstrate that, if n is the number of degrees of freedom of the structure, p the number of processors used in the computations, and s ≥ p is the number of subdomains in the partition, the net speed-up is \documentclass{article}\pagestyle{empty}\begin{document}$ O\left({p\sqrt s} \right) $\end{document} in 2D and O(ps) in 3D, asymptotically as n/s → ∞. In particular, speed-ups with respect to Newmark's method of \documentclass{article}\pagestyle{empty}\begin{document}$ O\left({p\sqrt s} \right) $\end{document} in 2D and O(s) in 3D are obtained on a single-processor machine. Finally, simulations on a 32-node hypercube are presented for which the interprocessor communication efficiencies obtained are consistently in excess of 90 per cent.
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  • 73
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2813-2827 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new element - a partial hybrid stress element - is proposed in this paper for the analysis of thick laminated composite plates. The variational principle of this element can be derived from the Hellinger-Reissner principle through dividing six stress components into a flexural part (σx, σy, σxy, σz) and a transverse shear part (τxy, τyz). The element stiffness matrix can be formulated by assuming a stress field only for transverse shear stresses, while all the others are obtained from an assumed displacement field. Consequently, this new element combines the benefits of the conventional displacement method and the hybrid stress method. A twenty-node hexahedron element is employed in each layer for the displacement field. For the assumed transverse shear stress field, only the traction-free boundary conditions and interface traction continuity are satisfied. The equilibrium equation is enforced by the variational principle. Hence, the complicated work of searching an equilibrating stress field for all the six stress components in the hybrid stress method can be avoided. Furthermore, the interlaminar traction discontinuity, especially transverse shear, encountered by the conventional displacement method and higher-order plate element for laminated plate analysis can also be overcome. Examples are illustrated to demonstrate the accuracy and efficiency of this proposed partial hybrid stress element.
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  • 74
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2877-2888 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Exact postbuckling stresses usually vary fairly smoothly. Unfortunately, finite element postbuckling stresses tend to be much less well behaved. The result is that second order postbuckling constants determined by the finite element method may be highly inaccurate. The reason is that in finite element solutions transverse displacements associated with the buckling fields furnish too rapidly varying postbuckling strain contributions, while the postbuckling axial or membrane displacements contribute strain components that are sufficiently smooth, thus creating an internal postbuckling strain and stress mismatch.The present study suggests a modified finite element method that handles the problem, which is a special example of membrane locking, by introducing the postbuckling strains as independent variables. In general, the method provides rather complicated finite element expressions. However, by a suitable choice of interpolating functions, the resulting finite element equations themselves may be found to be the usual ones, and yet provide smooth postbuckling stresses and therefore good values of the postbuckling constants.
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  • 75
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    International Journal for Numerical Methods in Engineering 27 (1989) 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 76
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 153-167 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Jacket structures have many sub-sea appurtenances attached to them. For example, a typical jacket structure has hundreds of sacrificial anodes attached to its members. The fluid loading of these structures is calculated using Morison's formula. The force coefficients used in the equation are based on experiments carried out, for the most part, on single cylinders in laboratories in ideal flow. There are very few experimental data available for the fluid loading of cylinders with appurtenances. Moreover, it is not possible to calculate theoretically the loading on such configurations in flows of practical interest using approaches such as the finite element method. However, appurtenances can contribute significantly to the overall loading on a structure. This paper presents the preliminary theoretical work that has been carried out as part of a programme to establish a rational method for estimating the fluid loading on tubular members with appurtenances.
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  • 77
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    International Journal for Numerical Methods in Engineering 27 (1989) 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 78
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 257-269 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The multilayer degenerated 3-D shell element, for which the stiffness matrix is calculated by numerical integration ply by ply, gives accurate results even for moderately thick composite panels, but needs an important linear and non-linear generation time when the number of plies is high (N 〉 10). In order to reduce this time some pre-integration and homogenization techniques are investigated. Different pre-integration methods have been reviewed and discussed. Then, two new homogenization methods, based on a series expansion of an equivalent Hooke matrix over the thickness, are presented. Next, simple numerical validation tests of these new methods are discussed, indicating necessary further investigations. Finally, a general discussion about the properties of the pre-integration and homogenization techniques tends to define their respective application domain.
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  • 79
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 343-359 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper deals with the static and dynamic (free vibrations) analysis of plates built up with a symmetric series of orthotropic layers. The formulation of a new simple triangular finite element having three nodes and three degrees of freedom per node is presented. The element called DST (Discrete Shear Triangle) is free of shear locking and has a proper rank. It coincides with DKT (Discrete Kirchhoff Triangle) when the transverse shear effects are negligible. A large number of classical problems is considered to evaluate the performance of the element for the analysis of composite plates. Very satisfactory results are obtained for displacements, stresses and frequencies.
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  • 80
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    International Journal for Numerical Methods in Engineering 27 (1989), S. 403-427 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In general, plates and shells out of composite laminates are more susceptible to transverse shear than those out of homogeneous isotropic material. By means of different shear flexible theories the effect on buckling and postbuckling behaviour is studied. It turns out that there is a rather limited range of plates where the transverse shear is of considerable influence. Results obtained with Mindlin- or Reissner-type theories prove almost as adequate as those obtained with Reddy's theory. For the buckling analysis of shells the Kirchhoff-Love theory is precise enough. Snap-through buckling and imperfection sensitivity appear in composite shells, too, especially if they are optimized with respect to high bifurcation buckling loads.
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  • 81
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 27-41 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Owing to the failure of the finite element analyst to employ a properly refined computational model, the accuracy of preliminary finite element computations is often low. Thus, it is useful to introduce a computational procedure for improving the results obtained from a preliminary finite element solution. Such a procedure is presented in this paper. In this procedure the solution error components are decomposed into two parts. One of the error components is assumed to have a long period variation. The other component is assumed to have a short period variation. Multiplicative and additive correction procedures are introduced to iteratively eliminate the two error components. The multiplicative and additive correction procedures are implemented using multi-level solution techniques.
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  • 82
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 181-191 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The dynamic substructure method is extended to multilevel (recursive) substructures. The obvious distinction of the two approaches is that the stiffness and mass matrices before condensation are no longer frequency independent. The dynamic stiffness matrix at any substructure level is proved to be a function of the vibrating frequency in terms of some constant matrices which are derivable from the dynamic stiffness matrix at one lower substructure level. The method can accurately predict more modes than the number of degrees of freedom retained. The computational procedure, the generalized inverse iteration, the stationary principle of the system natural frequency and the generalized Rayleigh's quotient are derived for the frequency dependent matrices. Numerical examples are given to illustrate some engineering applications. A transcendental dynamic stiffness matrix can be transformed to a more convenient algebraic form by the present method.
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  • 83
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 955-965 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A method is presented for determining the natural frequencies and the corresponding mode shapes of isotropic bimodular beams at certain supporting conditions. Although only the lowest three modes of the single-layer and two-layer bimodular beams were studied, in principle, the method may also be used to determine higher-mode natural frequencies and shapes of the multi-layer beams. Numerical analysis shows that the approach is suitable for the free-vibration analysis of clamped/free and hinged/hinged bimodular beams.
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  • 84
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 980-980 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 85
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1061-1076 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A general finite element is derived for beams or beam-columns with or without a continuous Winkler type elastic foundation. The need to discretize members into shorter elements for convergence towards an ‘exact’ solution is eliminated by employing in the derivation of the element exact shape functions obtained from the equation of the elastic line. Inter-nodal values of deflections, bending moments and shear forces are obtained using the exact shape functions and trigonometric series. The effect of heavy compressive or tensile axial forces on bending stiffness is treated as a linear problem by considering the axial force as a constant parameter affecting the stiffness. FORTRAN subroutines to compute the stiffness matrix, equivalent nodal forces, deflected shape, bending moments and shear forces are provided and verified by an example.
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  • 86
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1131-1144 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper we present the boundary integral formulation for the solution of quasi-static problems of two-dimensional uncoupled thermoelasticity. A detailed scheme for the numerical solution of the boundary integral equations and computation of internal stresses by regularized integral representation is developed in time formulation. The method is tested in a numerical example.
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  • 87
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1145-1160 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An effective arbitrary quadrilateral thin plate bending element with a quasi-conforming, QCQ element, is presented in this paper. The elements pass the patch test with constant strain and the patch test with linear strains approximately. When the element degenerates to a rectangle the patch test with linear strains is passed. The calculation of the element stiffness matrix is simple without numerical integration. The numerical examples show that the QCQ element has a higher accuracy and a faster convergence rate.
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  • 88
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1371-1387 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An efficient and simple infinite element for modelling the far field potential of water wave diffraction problems is presented. The shape functions in the radial direction comprise the first two terms of the asymptotic expansions of Hankel functions. The integrals with infinite limit for calculating the coefficient matrix have been worked out. Numerical tests on the diffraction by a surface-piercing circular cylinder give surprisingly accurate resultant forces even if the infinite elements are placed very near to the cylinder. Other typical three dimensional examples also show that satisfactory results can be obtained by the use of this simple infinite element. A computer program, WALOAD, has been developed for computing the wave forces on fixed two and three dimensional bodies.
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  • 89
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1449-1460 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An element formulation is suggested to obtain variable order singularities simultaneously at the two neighbouring corner nodes of a 4-noded quadrilateral. The element is useful for modelling a kinked crack with small kink length. The accuracy is good and this is obtained at a substantially reduced computational cost. Case studies are presented to illustrate the usefulness and the accuracy obtainable.
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  • 90
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1599-1618 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A finite element method based on the virtual work principle to determine the steady state response of frams in free or forced periodic vibration is introduced. The axial and flexural deformations are coupled by mean of the induced axial force along the element. The spatial discretization of the deformations is achieved by the usual finite element method and the time discretization by Fourier coefficients of the nodal displacements. No unconventional element matrices are needed. After applying the harmonic balance method, a set of non-linear algebraic equations of the Fourier coefficients is obtained. These equations are solved by the Newtonian iteration method in terms of the Fourier coefficient increments. Nodal damping can easily be included by a diagonal damping matrix. The direct numerical determination of the Fourier coefficient increments is difficult owing to the presence of peaks, loops and discontinuities of slope along the amplitude-frequency response curves. Parametric construction of the response curves using the phase difference between the response and excitation is recommended to provide more points during the rapid change of the phase (i.e. at resonance). For undamped natural vibration, the method of selective coefficients adopted.Numerical examples on the Duffing equation, a hinged-hinged beam, a clamped-hinged beam, a ring and a frame are given. For reasonably accurate results, it is shown that the number of finite elements must be sufficient to predict at least the linear mode at the frequency of interest and the number of harmones considered must satisfy the conditions of completeness and balanceability, which are discussed in detail.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1703-1713 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: New fundamental solutions which automatically satisfy boundary conditions at the interfaces of an elastic plate perfectly bonded to two elastic halfspaces are implemented in a 3-D boundary element method (BEM) for crack problems. The BEM features a new integration scheme for highly singular kernels. The capability is achieved through a part analytic and part numerical integration procedure, such that the analytic part of the integration is similar for all slip/opening variations, ‘Part-through’ elliptic cracks in an elastic plate with traction-free surfaces are analysed and the stress intensity factor (SIF) values along the crack front are found to compare favourably with widely accepted numerically obtained SIF results by Raju and Newman.1
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    International Journal for Numerical Methods in Engineering 28 (1989) 
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    Keywords: Engineering ; Engineering General
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  • 93
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1787-1801 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: We present in this paper a procedure to establish Reissner-Mindlin plate bending elements. The procedure is based on the idea to combine known results on the approximation of Stokes problems with known results on the approximation of elliptic problems. The proposed elements satisfy the mathematical conditions of stability and convergence, and some of them promise to provide efficient elements for practical solutions.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 1827-1837 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Two-sided Ostrowski matrix iteration is applied to solve eigenproblems of non-conservative continuous systems in structural dynamics starting from known eigensolutions of a related conservative system. Eigensolution paths are followed as the parameters of the system governing its non-conservative behaviour are increased from zero. No discretization is performed and no truncation errors are introduced. The real eigenvalues of the related conservative problem are calculated by use of the Wittrick-Williams algorithm. The corresponding eigenvectors are obtained with an inverse iteration method using eigenfrequency-dependent trial eigenvectors. Residues of system harmonic transfer functions are computed using the eigenvalues and eigenvectors together with eigenvector normalization constants (modal Foss dampings). These residues are needed in a transient analysis. The method is applied to the generalized Beck column stability problem. Critical loads are calculated. Harmonic flexibilities of subcritically loaded columns in transverse vibration are studied. Applications to control problems are foreseen.
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    International Journal for Numerical Methods in Engineering 28 (1989) 
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    Keywords: Engineering ; Engineering General
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  • 96
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2021-2039 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A combination of the characteristics method and finite elements techniques is applied to solve a coupled heat transfer-chemical reaction system in the stationary case. The solution of the non-linear discretized system is obtained by using Newton's algorithm. Finally, numerical results for several problems are presented to test the accuracy and stability of the method.
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  • 97
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2583-2607 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The objective of the research presented here was to develop a generic adaptive computational method for porous media evolution problems that involve coupled heat flow, fluid flow and species transport processes with sharply defined phase-change interfaces.In this paper we examine the general least squares variational approach and develop the conceptual framework for a rate least squares variational formulation of a continuously deforming mixed variable finite element method for solving highly non-linear time-dependent partial differential equations. In Part II of this paper1 we extend the formulation given here for a single evolution equation to a system of coupled evolution equations. In Part III2 we discuss in detail the numerical procedures that were implemented in a computer program and present several numerical examples that demonstrate the performance of this computational method.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2681-2693 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The purpose of this article is to review the publications dealing with the application of finite elements in the thermoforming process. Although no attempt has been made to discuss the theoretical aspects of these publications, their general methodologies have been presented. The paper is particularly aimed at readers who are seeking an overall view of the subject and possibly interested in acquiring a CAD system for thermoforming technology.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2651-2679 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A FORTRAN 77 program for reducing the profile and wavefront of a sparse matrix with a symmetric structure is described. The implementation is based on an algorithm published previously by the Author and appears in response to a large number of enquiries for the source code. Extensive testing of the scheme suggests that its performance is consistently superior to that of the widely used reverse Cuthill-McKee and Gibbs-King methods. In addition to presenting a complete listing of the program, we also describe how to interface it with a typical finite element code. The scheme is especially useful in finite element analysis where it can be employed to derive efficient orderings for both profile and frontal solution schemes.
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    International Journal for Numerical Methods in Engineering 28 (1989), S. 2710-2710 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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