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  • 1
    Electronic Resource
    Electronic Resource
    Katacalcin — Ein neuer Tumormarker beim C-Zell-Karzinom der Schilddrüse (1987)
    Springer
    Journal of molecular medicine 65 (1987), S. 82-86 
    Details
    ISSN: 1432-1440
    Keywords: Katacalcin ; Tumor marker ; C-cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Katacalcin (KC) is situated on the C-terminal side of the procalcitonin molecule and is cleaved like calcitonin (CT) from this precursor peptide. Serum levels of KC were measured in 22 patients with C-cell carcinoma with a specific and sensitive radioimmunoassay (normal range, 〈0.1–0.15 ng/ml). Basal serum KC values in C-cell carcinoma patients were 0.32-290 ng/ml. There was a good correlation between KC and CT (r=0.98,P〈0.001). Serum KC, as well as CT, markedly increased after pentagastrin and calcium infusion. KC and CT were secreted in nearly equimolar amounts. During selective venous catheterization, KC and CT levels were increased in serum samples from veins draining tumor masses, which could be confirmed operatively. During the follow up, KC and CT measurements correlated well to the stage of disease. KC could be immunohistologically localized in C-cell carcinoma tissue. As a tumor marker, katacalcin is likely to be as useful as calcitonin in C-cell carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01745480
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  • 2
    Electronic Resource
    Electronic Resource
    Role of voltage-dependent calcium channels in secretion of calcitonin from human medullary thyroid carcinoma cells (1989)
    Springer
    Journal of molecular medicine 67 (1989), S. 635-639 
    Details
    ISSN: 1432-1440
    Keywords: Voltage dependent calcium channels ; Calcitoninsecretion ; Human medullary thyroid carcinoma ; BAY K 8644-nifedipine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Extracellular calcium concentration is an important regulator of calcitonin secretion. We used primary cell cultures of human medullary thyroid carcinoma to study the role of voltage dependent calcium channels for stimulus secretion coupling. Increasing extracellular calcium concentration (1.6–5.0 mM) in the medium caused a dose dependent release of calcitonin. The calcium channel activator BAY K 8644, a dihydropyridine, stimulated calcitonin secretion in a dose dependent manner (10−7−10−5 M). This effect was completely inhibited by equimolar concentrations of the calcium channel blocker nifedipine and abolished in the absence of extracellular calcium. Similarly, nifedipine suppressed the stimulatory action of extracellular calcium. The effects of calcium and BAY K 8644 with and without nifedipine suggest that calcium influx via voltage dependent calcium channels plays an important role in calcitonin secretion. The primary cell culture of human medullary thyroid carcinoma is a good model for the study of stimulus secretion coupling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718147
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  • 3
    Electronic Resource
    Electronic Resource
    Respiratory distress as primary symptom of relapse in a patient with non-hodgkin lymphoma (1985)
    Springer
    Annals of hematology 51 (1985), S. 287-289 
    Details
    ISSN: 1432-0584
    Keywords: Immunoblastic lymphoma ; Respiratory distress ; Endotracheal lymphoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A report on a 74-year old woman with high-grade malignant non-Hodgkin lymphoma in clinical remission, who suddenly developed episodes of severe dyspnea and wheezing. Clinical and roentgenographic investigations including tomography of the trachea failed to demonstrate any lesion. Fiberoptic bronchoscopy revealed and endotracheal, polyp-like lymphoma 3 cm above the carina, which was immediately operated. Other sites of relapse (submandibular lymphnodes) were found 2 weeks after the operation. Second-line chemotherapy (IMVP 16) was able to induce a second remission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320523
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  • 4
    Electronic Resource
    Electronic Resource
    Secretion of calcitonin and carcinoembryonic antigen in long-term organ culture of human medullary thyroid carcinoma: Biochemical and immunocytochemical studies (1985)
    Springer
    Journal of molecular medicine 63 (1985), S. 205-210 
    Details
    ISSN: 1432-1440
    Keywords: Calcitonin ; Carcinoembryonic antigen ; Medullary thyroid carcinoma in vitro ; Organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tissue cultures of four C-cell carcinomas (medullary thyroid carcinoma, MTC) were prepared to study the basal and stimulated calcitonin (CT) and carcinoembryonic antigen (CEA) release. Immunohistological staining of the explants for CT and CEA have been performed after various periods of culture. These MTC explants were able continuously to release CT and CEA for periods up to 157 days. The spontaneous CT and CEA release decreased sharply during the 1st week of culture, then remained nearly constant over the observation period. The CEA/CT secretion ratio slightly declined during long-term culture; CEA release seems to drop earlier than CT production. CT and CEA could be detected in the same cells by immunocytochemical technique. The septal tissue consisting of dense connective tissue and amyloid produced by tumor cells seemed to increase during long-term culture. CT, but not CEA, was stimulated by pentagastrin (10−5 M), glucagon (6×10−6 M), and dose related by calcium (2.5–20 mM) in vitro. The MTC explant organ long-term culture proved to be a useful model for studies of human CT and CEA secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01731170
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  • 5
    Electronic Resource
    Electronic Resource
    Renin immunocytochemistry of the differentiating juxtaglomerular apparatus (1981)
    Springer
    Anatomy and embryology 162 (1981), S. 173-181 
    Details
    ISSN: 1432-0568
    Keywords: Renin ; Juxtaglomerular apparatus ; Immunocytochemistry ; Fetal kidney ; Renin-angiotensin-system (RAS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The differentiation of the juxtaglomerular apparatus in fetuses and newborn mice was investigated by renin immunocytochemistry and electron microscopy. Three to four days before delivery and prior to other organs renin was found in the fetal kidney. At this early time immunoreactivity was preferentially located in cells of the media of interlobular arteries. In newborn mice the formation of new nephrons and maturation of their glomeruli was accompanied by a shift in renin localization from the interlobular arteries to the afferent arterioles. At the same time, kidney renin content and concentration increased rapidly. Synchronously with renin immunoreactivity, during the capillary loop stage of glomerular development, granulated epitheloid cells became visible in the afferent arteriole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306489
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  • 6
    Electronic Resource
    Electronic Resource
    Co-localization of xenopsin and gastrin immunoreactivity in gastric antral G-cells (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 135-138 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Studies indicating evidence for the presence of the amphibian octapeptide xenopsin in gastric mucosa of mammals prompted us to investigate the cellular localization of this peptide. Using the peroxidase-antiperoxidase method and a specific antiserum to xenopsin (Xen-7) on paraffin and adjacent semithin sections of gastric antral mucosa from man, dog, and Tupaia belangeri, we found numerous epithelial cells showing a specific positive immunoreaction. These cells were of a typical pyramidal shape and could be classified as of the “open” type. Cell quantification in serial sections processed for xenopsin and gastrin immunoreactivity, respectively, revealed an identical number of cells per section and an identical distribution of these cells in the middle zone of the antral mucosa. Furthermore, adjacent semithin sections demonstrated the colocalization of xenopsin and gastrin immunoreactivity within the same G-cell. The xenopsin antiserum could be completely absorbed with synthetic xenopsin but not with gastrin. Preabsorption tests with neurotensin, a xenopsin related peptide, or with somatostatin, glucagon, and enkephalins gave no evidence for crossreactivity of the xenopsin antiserum with these peptides. It is concluded that gastric antral G-cells in addition to gastrin also contain the amphibian peptide xenopsin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491760
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  • 7
    Electronic Resource
    Electronic Resource
    Renin in the uterus of pregnant mice immunocytochemical, ultrastructural and biochemical studies (1980)
    Springer
    Histochemistry and cell biology 68 (1980), S. 253-263 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and content of renin in the uterus of pregnant mice (12–14 days of gestation) was studied by immunocytochemistry, electron microscopy and radioimmunoassay. The uterine renin concentration at this stage of pregnancy was four-fold higher than that of the nonpregnant uterus but still less than 0.1% of the kidney renin concentration. Renin was localized almost exclusively in the decidual and entodermal epithelial linings of the yolk sac (uterine) cavity near the marginal sections of the placenta. By electron microscopy granular and vesicular structures were observed in the renin containing epithelial cells. Chorioallantoic placenta, myometrium, decidua basalis and the antimesometral parts of the epithelial leafs of the uterine cavity did not contain renin. The specific localization of renin suggests a role in parturition or delivery of the placenta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493255
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  • 8
    Electronic Resource
    Electronic Resource
    Different effects of hypercalcemic state induced by walker tumor (HWCS 256) and 1,25 (OH)D3 intoxication on rat thyroid C cells (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 503-508 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two different models of chronic C cell stimulation by the hypercalcemic state were compared with respect to their morphology, immunocytochemistry, and biochemistry. In the chronic hypercalcemic state due to the HWCS 256 strain of the Walker tumor C cells show signs of degeneration such as vacuolation, on day 7 after tumor implantation. On day 10 tumor induced hypercalcemia leads to irreversible cell damage with karyopyknosis and karyorhexis. These morphological changes are accompanied by a decline in radioimmunologically measurable calcitonin content of the thyroid and by the loss of response to acute stimulation of C cells. In contrast, in the hypercalcemic state due to 1,25(OH)2D3 intoxication we find an almost complete degranulation of C cells but no signs of degeneration or cell damage, although the thyroid calcitonin content and the calcitonin secretion capacity is greatly reduced. Tumor induced cachexia as a reason for C cell damage in tumor bearing rats could be excluded. Other possible reasons, such as acute overstimulation and tumor factors acting directly on C cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495442
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  • 9
    Electronic Resource
    Electronic Resource
    Freeze-fracture autoradiography: The in-vacuo coating technique (1978)
    Springer
    Histochemistry and cell biology 59 (1978), S. 9-16 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Freeze-fracture autoradiography (FFA) was introduced in 1976 as a new method for electron microscopic autoradiography of diffusible compounds (Fisher and Branton, Rix et al.). With the original technique, the film monolayer was applied to the cold specimen in a cryostat at atmospheric pressure. Coating under these conditions did not exclude the risk of artifacts, mainly due to uncontrolled ice contamination of the cold specimen surface. A new method has been developed for coating the frozen specimen, immediately after replication, in the maintained vacuum of the freeze-fracture unit. Two main components of the new technique are described in detail, a specially designed coating device, and the use of spreading substances promoting adhesion of the film in vacuo. Using this technique artifacts so far inherent in the FFA method can be eliminated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00506473
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  • 10
    Electronic Resource
    Electronic Resource
    Renin in the uterus of non-pregnant mice (1980)
    Springer
    Histochemistry and cell biology 66 (1980), S. 229-238 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of renin in the uterus of non-pregnant mice was studied by immunocytochemistry, using the peroxidase-antiperoxidase (PAP) technique, by electron microscopy and biochemical renin estimation. By immunocytochemistry, two separate localizations of renin could be identified, one in the apical region of the endometrial cells, the other in perivascular cells of the myometrium. The renin concentration in the endometrium, as determined cytochemically, and the total renin content of the uterus, as estimated enzymatically, varied with the ovarian cycle, being highest during the oestrus and lowest during the post-oestrus phase. The electronmicroscopic finding of fluctuations in the granule content of the epithelial cells was in accordance with the immunocytochemical and biochemical data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495736
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