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  • 11
    Digitale Medien
    Digitale Medien
    The expression of a small heat shock protein homologue is developmentally regulated in Nippostrongylus brasiliensis
    Amsterdam : Elsevier
    Molecular & Biochemical Parasitology 61 (1993), S. 149-153 
    Details
    ISSN: 0166-6851
    Schlagwort(e): Nematode ; Nippostrongylus brasiliensis ; Small heat shock protein ; [abr] HSP; heat shock protein ; [abr] PCR; polymerase chain reaction ; [abr] p.i.; post-infection
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0166-6851(93)90168-W
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  • 12
    Digitale Medien
    Digitale Medien
    Sequence, expression and evolution of the globins of the parasitic nematode Nippostrongylus brasiliensis
    Amsterdam : Elsevier
    Molecular & Biochemical Parasitology 68 (1994), S. 1-14 
    Details
    ISSN: 0166-6851
    Schlagwort(e): Caenorhabditis ; Evolution ; Globin ; Intron ; Nematode ; Nippostrongylus ; [abr] GLBB; body globin isoform ; [abr] GLBC; cuticular globin isoform ; [abr] L3i; infective third stage larvae ; [abr] L4; fourth stage larva ; [abr] PBS; phosphate buffered saline ; [abr] PI; protease inhibitor cocktail ; [abr] RT-PCR; reverse transcriptase polymerase chain reaction ; [abr] SL; spliced leader ; [abr] nOG; n-octyl glucoside
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0166-6851(94)00127-8
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  • 13
    Digitale Medien
    Digitale Medien
    Primary rhabdoid tumour of the brain (1988)
    Springer
    Virchows Archiv 412 (1988), S. 393-397 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Rhabdoid tumours ; Primary brain tumours ; Intermediate filaments ; Wilms' and related tumours ; Vimentin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A posterior fossa tumour in a 3 year old child is presented with characteristic histological, ultrastructural and immunohistochemical features of rhabdoid tumour. Many tumour cells contained cytoplasmic eosinophilic hyaline inclusions. Ultrastructurally concentric whorls of 10 nm intermediate filaments were identified. Immunohistochemical staining disclosed vimentin, cytokeratin and epithelial membrane antigen positivity. Renal and extrarenal rhabdoid tumours have been well documented but a primary rhabdoid tumour of the brain is extremely rare. Additional ultrastructural features seen were tubular crystalline inclusions in endoplasmic reticulum and abnormal large mitochondria.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00750268
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  • 14
    Digitale Medien
    Digitale Medien
    Studies on the vaporization of pentachlorophenol from treated wood (1986)
    Springer
    Archives of environmental contamination and toxicology 15 (1986), S. 669-676 
    Details
    ISSN: 1432-0703
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik , Medizin
    Notizen: Abstract Studies were made with pentachlorophenol (PCP) to determine the effects of temperature, air flow, humidity, carrier solvents, and wood coating systems on the vaporization properties of PCP. Temperature had the greatest effect on PCP vaporization from treated wood. Methylene chloride was associated with the highest air concentrations of PCP. The cosolvent WC-144 was most effective in reducing PCP vaporization, and a bituminous coating and an epoxy paint were the most effective coatings for reducing PCP vaporization from treated wood.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01054914
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  • 15
    Digitale Medien
    Digitale Medien
    Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 667-674 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Gene libraries (“zoolibraries”) were constructed in Escherichia coli using DNA isolated from the mixed liquor of thermophilic, anaerobic digesters, which were in continuous operation with lignocellulosic feedstocks for over 10 years. Clones expressing cellulase and xylosidase were readily recovered from these libraries. Four clones that hydrolyzed carboxymethylcellulose and methylumbelliferyl-β-d-cellobiopyranoside were characterized. All four cellulases exhibited temperature optima (60–65° C) and pH optima (pH 6–7) in accordance with conditions of the enrichment. The DNA sequence of the insert in one clone (plasmid pFGH1) was determined. This plasmid encoded an endoglucanase (celA) and part of a putative β-glucosidase (celB), both of which were distinctly different from all previously reported homologues. CelA protein shared limited homology with members of the A3 subfamily of cellulases, being similar to endoglucanase C from Clostridium thermocellum (40% identity). The N-terminal part of CelB protein was most similar to β-glucosidase from Pseudomonas fluorescens subsp. cellulosa (28% homology). The use of zoolibraries constructed from natural or laboratory enrichment cultures offers the potential to discover many new enzymes for biotechnological applications.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00164771
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  • 16
    Digitale Medien
    Digitale Medien
    Improved strains of recombinant Escherichia colifor ethanol production from sugar mixtures (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 70-75 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract  Hemicellulose hydrolysates of agricultural residues often contain mixtures of hexose and pentose sugars. Ethanologenic Escherichia coli that have been previously investigated preferentially ferment hexose sugars. In some cases, xylose fermentation was slow or incomplete. The purpose of this study was to develop improved ethanologenic E. coli strains for the fermentation of pentoses in sugar mixtures. Using fosfomycin as a selective agent, glucose-negative mutants of E. coli KO11 (containing chromosomally integrated genes encoding the ethanol pathway from Zymomonas mobilis) were isolated that were unable to ferment sugars transported by the phosphoenolpyruvate-dependent phosphotransferase system. These strains (SL31 and SL142) retained the ability to ferment sugars with independent transport systems such as arabinose and xylose and were used to ferment pentose sugars to ethanol selectively in the presence of high concentrations of glucose. Additional fosfomycin-resistant mutants were isolated that were superior to strain KO11 for ethanol production from hexose and pentose sugars. These hyperproductive strains (SL28 and SL40) retained the ability to metabolize all sugars tested, completed fermentations more rapidly, and achieved higher ethanol yields than the parent. Both SL28 and SL40 produced 60 g l-1 ethanol from 120 g l-1 xylose in 60 h, 20% more ethanol than KO11 under identical conditions. Further studies illustrated the feasibility of sequential fermentation. A mixture of hexose and pentose sugars was fermented with near theoretical yield by SL40 in the first step followed by a second fermentation in which yeast and glucose were added. Such a two-step approach can combine the attributes of ethanologenic E. coli for pentoses with the high ethanol tolerance of conventional yeasts in a single vessel.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530050372
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  • 17
    Digitale Medien
    Digitale Medien
    Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 667-674 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract  Gene libraries (“zoolibraries”) were constructed in Escherichia coli using DNA isolated from the mixed liquor of thermophilic, anaerobic digesters, which were in continuous operation with lignocellulosic feedstocks for over 10 years. Clones expressing cellulase and xylosidase were readily recovered from these libraries. Four clones that hydrolyzed carboxymethylcellulose and methylumbelliferyl-β-D-cellobiopyranoside were characterized. All four cellulases exhibited temperature optima (60–65 °C) and pH optima (pH 6–7) in accordance with conditions of the enrichment. The DNA sequence of the insert in one clone (plasmid pFGH1) was determined. This plasmid encoded an endoglucanase (celA) and part of a putative β-glucosidase (celB), both of which were distinctly different from all previously reported homologues. CelA protein shared limited homology with members of the A3 subfamily of cellulases, being similar to endoglucanase C from Clostridium thermocellum (40% identity). The N-terminal part of CelB protein was most similar to β-glucosidase from Pseudomonas fluorescens subsp. cellulosa (28% homology). The use of zoolibraries constructed from natural or laboratory enrichment cultures offers the potential to discover many new enzymes for biotechnological applications.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00164771
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  • 18
    Digitale Medien
    Digitale Medien
    Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 70-75 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Hemicellulose hydrolysates of agricultural residues often contain mixtures of hexose and pentose sugars. Ethanologenic Escherichia coli that have been previously investigated preferentially ferment hexose sugars. In some cases, xylose fermentation was slow or incomplete. The purpose of this study was to develop improved ethanologenic E. coli strains for the fermentation of pentoses in sugar mixtures. Using fosfomycin as a selective agent, glucose-negative mutants of E. coli KO11 (containing chromosomally integrated genes encoding the ethanol pathway from Zymomonas mobilis) were isolated that were unable to ferment sugars transported by the phosphoenolpyruvate-dependent phosphotransferase system. These strains (SL31 and SL142) retained the ability to ferment sugars with independent transport systems such as arabinose and xylose and were used to ferment pentose sugars to ethanol selectively in the presence of high concentrations of glucose. Additional fosfomycin-resistant mutants were isolated that were superior to strain KO11 for ethanol production from hexose and pentose sugars. These hyperproductive strains (SL28 and SL40) retained the ability to metabolize all sugars tested, completed fermentations more rapidly, and achieved higher ethanol yields than the parent. Both SL28 and SL40 produced 60 gl−1 ethanol from 120 gl−1 xylose in 60 h, 20% more ethanol than KO11 under identical conditions. Further studies illustrated the feasibility of sequential fermentation. A mixture of hexose and pentose sugars was fermented with near theoretical yield by SL40 in the first step followed by a second fermentation in which yeast and glucose were added. Such a two-step approach can combine the attributes of ethanologenic E. coli for pentoses with the high ethanol tolerance of conventional yeasts in a single vessel.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00170625
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  • 19
    Digitale Medien
    Digitale Medien
    Effects of process errors on the production of ethanol by Escherichia coli KO11 (1998)
    Springer
    Journal of industrial microbiology and biotechnology 20 (1998), S. 281-286 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Keywords: lignocellulose; biomass; fermentation; ethanol; E. coli KO11; xylose; process errors; process upsets
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Escherichia coli KO11 was previously constructed for the production of ethanol from both hexose and pentose sugars in hemicellulose hydrolysates by inserting the Zymomonas mobilis genes encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB). This biocatalyst appears relatively resistant to potential process errors during fermentation. Antibiotics were not required to maintain the maximum catabolic activity of KO11 even after deliberate contamination with up to 10% soil. Fermentations exposed to extremes of temperature (2 h at 5°C or 50°C) or pH (2 h at pH 3 or pH 10) recovered after re-adjustment to optimal fermentation conditions (35°C, pH6) although longer times were required for completion in most cases. Ethanol yields were not altered by exposure to extremes in temperature but were reduced by exposure to extremes in pH. Re-inoculation with 5% (by volume) from control fermentors reduced this delay after exposure to pH extremes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/sj.jim.2900524
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  • 20
    Digitale Medien
    Digitale Medien
    Unsaturated fatty acid requirement inEscherichia coli: Mechanism of palmitate-induced inhibition of growth of strain WN1 (1982)
    Springer
    The journal of membrane biology 65 (1982), S. 31-40 
    Details
    ISSN: 1432-1424
    Schlagwort(e): lipids ; membranes ; Escherichia coli ; temperature adaptation ; fatty acids ; phase separations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary The minimum requirement for unsaturated fatty acids was investigated inE. coli using a mutant impaired in the synthesis of vaccenic acid. Exogenously supplied palmitic acid was incorporated by this mutant which led to a reduction in the proportion of cellular unsaturated fatty acids. Growth was impaired as the level of saturated fatty acids approached 76% at 37°C and 60% at 30°C. The basis of this growth inhibition was investigated. Most transport systems and enzymes examined remained active in palmitate-grown cells although the specific activities of glutamate uptake and succinic dehydrogenase were depressed 50%. Fluorescent probes of membrane organization indicated that fluidity decreased with palmitate incorportation. Temperature scans with parinaric acid indicated that rigid lipid domains exist in palmitategrown cells at their respective growth temperature. Freeze-fracture electron microscopy confirmed the presence of phase separations (particle-free areas) in palmitate-grown cells held at their growth temperature prior to quenching. The extent of this separation into particle-free and particle-enriched domains was equivalent to that induced by a shift to 0°C in control cells. The incorporation of palmitate increased nucleotide leakage over threefold. The cytoplasmic enzyme β-galactosidase was released into the surrounding medium as the concentration of unsaturated fatty acid approached the minimum for a particular growth temperature. Lysis was observed as a decrease in turbidity when cells which had been grown with palmitate were shifted to a lower growth temperature. From these results we propose that leakage and partial lysis are the major factors contributing to the apparent decrease in growth rate caused by the excessive incorporation of palmitate. Further, we propose that membrane integrity may determine the minimum requirement for unsaturated fatty acids inE. coli rather than a specific effect on membrane transport and/or membrane-bound enzymes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01870466
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