ISSN:
1432-1424
Keywords:
Erythrocyte
;
Cytoskeleton
;
Cell deformability
;
Anion transport inhibitors
;
Band 3
;
Sickle cell disease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Two nonpenetrating membrane probes, p-azidobenzylphlorizin (p-AzBPhz) and 4,4′-diisothiocyano-2,2′-stilbene disulfonate (DIDS), have been shown in earlier studies to induce dose-dependent changes in red blood cell (RBC) shape and volume at the same low concentrations that inhibit anion transport. In the present work, these ligand-induced morphology and rheology changes were studied using video digital image morphometry (VDIM) and microfiltration techniques. The results of these experiments corroborate our earlier investigation. RBCs were filmed using a Nomarski optics microscope with video camera attachment and cell size and shape changes were computer analyzed using VDIM. Low μm p-AzBPhz or DIDS levels caused collapse of the cell's biconcave structure and cell flattening occurred within 1–2 sec after drug exposure. Higher doses of either agent converted cells to a new steady-state in which a concurrent limited increase in erythrocyte volume and blunt membrane protrusions were produced. These changes were reversed in less than 2 sec by washing the drug from the membrane. Both ligands increased the deformability of RBCs in a dose-dependent manner as determined by filtration through Nuclepore polycarbonate filters (3 μm pore diameter). The improvement in deformability of drug-treated sickle cells was much more dramatic than for normal cells at low p-AzBPhz concentrations. These results support our earlier conclusions that the ligands, through a common interaction with band 3, induce volume-associated cytoskeletal alterations which lead to changes in morphology and flexibility.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00232877
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