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  • 1
    Electronic Resource
    Electronic Resource
    Colony stimulating activity in acute and chronic endotoxinemia in man (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1398-1400 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Blood granulocyte-macrophage colony stimulating activity (GM CSF) was measured in 6 normal individuals challenged with low-dose endotoxin and in 63 unselected patients with nonhaematological disorders. 5/63 patients were febrile and 5 other patients showed detectable endotoxin levels, as measured by the Limulus assay. CSA levels showed a rapid increase in normal individuals following endotoxin administration, but were in the normal range in patients with chronic endotoxinemia or in those with febrile disorders. Thus, unlike acute endotoxinemia, chronic endotoxinemia is not associated with elevated activity that promotes growth of myeloid commited stem cells. In addition, fever per se did not coincide with elevated blood CSA levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964032
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular weight and some chemical properties of the Granulocytic Chalone (1978)
    Springer
    Annals of hematology 37 (1978), S. 7-18 
    Details
    ISSN: 1432-0584
    Keywords: Granulopoese ; Chalon ; Koloniebildung ; Granulopoiesis ; Chalone ; Colony formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Granulocytic Chalone (GCh) has been highly purified from culture medium conditioned by human peripheral leukocytes. Purification was performed by column chromatography on Sephadex G-25 and G-10, rechromatography on G-10, preparative paper chromatography and thin-layer-chromatography. The inhibitory activity and specificity of GCh was monitored by agar colony formation and3H-thymidine incorporation into bone marrow and thymic cells. The gelchromatographic behavior of GCh is discussed in detail with regard to its molecular weight. The results obtained indicate that GCh may be a small (MW 500–600), acidic and highly polar peptide containing aspartic and glutamic acid among others. The N-terminal aminogroup seems to be blocked.
    Notes: Zusammenfassung Ein Peptid, welches sich wie ein Granulozytenchalon verhält, wurde aus durch normale menschliche Leukozyten konditioniertem Medium gewonnen. Die Reinigung erfolgte durch Säulenchromatographie an Sephadex G-25, G-10, Rechromatographie an G-10, Ionenaustauschchromatographie an Dowex 50, sowie durch präparative Papier- und Dünnschichtchromatographie. Die Hemmwirkung und Spezifität wurden mittels der Koloniebildung in Agar sowie durch3H-Thymidineinbau in Knochenmarks- und Thymuszellen nachgewiesen. Das gelchromatographische Verhalten von GCH wird im Hinblick auf das Molgewicht diskutiert. Demnach scheint es sich um ein saures Peptid vom Molgewicht 500–600 zu handeln, welches neben anderen Aminosäuren Asparaginsäure und Glutaminsäure enthält. Die N-terminale Aminogruppe scheint blockiert zu sein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01006548
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  • 3
    Electronic Resource
    Electronic Resource
    Human lung tissue as a source of colony stimulating activity (1978)
    Springer
    Annals of hematology 37 (1978), S. 69-74 
    Details
    ISSN: 1432-0584
    Keywords: Koloniestimulierende Aktivität ; Menschliches Lungen-gewebe ; Alveolar Makrophages ; Colony stimulating activity ; Human lung tissue ; Alveolar macrophages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Medium conditioned by human lung tissue was found to contain colony stimulating activity (CSA). This material was tested against mouse and human bone marrow as target system. Colony forming units (CFUc)from both species responded and gave rise to clonal growth in agar cultures. This colony formation was dose dependent and the relationship was a sigmoid one. Experiments to determine the molecular weight of human lung derived colony stimulating Factors brought evidence for four active molecular weight fractions with approximately 79000, 40000, 23000 and 2000 daltons. The 23000 dalton fraction activated human cells only, whereas the other fractions were active on both human and mouse bone marrow cells.
    Notes: Zusammenfassung Medium, welches durch menschliches Lungengewebe konditioniert wird, enthält kolonienstimulierende Aktivität (= Colony Stimulating Activity, CSA). Diese Präparation wurde an Menschen- und Mäuseknochenmark getestet. Kolonienbildende Einheiten von beiden Species wurden in Agarkulturen zu klonalem Wachstum aktiviert. Es zeigte sich eine sigmoide Dosisabhängigkeit des Wachtums der CFUc von der Konzentration an lungenkonditioniertem Medium. Die Molekulargewichtsbestimmung der kolonienstimulierenden Faktoren in Lungenmedium ergab vier Molekulargewichtsfraktionen, nämlich mit annähernd 79000,40000,23000und 2000 Daltons. Die 23 000 Dalton-Fraktion aktivierte ausschließlich menschliche Zellen, die übrigen Fraktionen aktivierten Menschen- und Mäuseknochenmarkzellen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002104
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  • 4
    Electronic Resource
    Electronic Resource
    Fatal Graft-versus-Host reaction following granulocyte transfusions (1982)
    Springer
    Annals of hematology 45 (1982), S. 115-119 
    Details
    ISSN: 1432-0584
    Keywords: Granulocyte transfusion ; Graft-versus-Host reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fatal Graft-versus-Host reactions (GVHR) were observed in a 5-year-old boy with Hodgkin's disease after application of non irradiated granulocytes derived from his mother and in a 59 year old female suffering from glioblastoma following transfusion of buffy coat cells from fresh blood units. Clinical signs as well as histopathological changes found in skin biopsies and subsequently at autopsy, were typical for GVHR. In one of the cases the sex difference between donor and recipient provided evidence for engraftment of donor lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319939
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of activated T cells and the suppressor/inducer subset in patients suffering from severe aplastic anemia (1989)
    Springer
    Annals of hematology 58 (1989), S. 21-26 
    Details
    ISSN: 1432-0584
    Keywords: SAA ; ATG/MP ; T cell subpopulations ; HLA-DR antigen expression suppressor/inducer subset
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In patients with severe aplastic anemia (SAA), lymphocyte subpopulations were examined for the presence of HLA-DR and 2H4 (suppressor/inducer subset) antigen-expressing cells by flow cytometric analysis. Investigations were performed on peripheral blood lymphocytes before and after therapy with antithymocyte globulin (ATG) and methylprednisolone (MP), as well as on bonemarrow lymphocytes before therapy. Before treatment, only the absolute numbers of CD4+ T cells and the CD4+HLA−DR+/CD8+HLA-DR+ activated T cell ratio were significantly decreased (p〈0.01 and p〈0.001, respectively). Following successful ATG/MP treatment, a decrease in the CD4+/CD8+T cell ratio was found. Regarding the suppressor/inducer subset, only absolute numbers of CD4+/2H4+ cells were somewhat higher in treated patients; the percentages were the same in all groups of patients. Studies performed on bonemarrow lymphocytes showed significantly decreased percentages of CD4+ and CD8+ T lymphocytes, which also express HLA-DR antigen. No significant changes in the distribution of activated T cells following ATG/MP therapy were found, suggesting that these cells play no major role in the pathogenesis of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320231
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of the target platelet glycoprotein in autoimmune thrombocytopenia occurring after allogeneic bone marrow transplantation (1989)
    Springer
    Annals of hematology 58 (1989), S. 151-153 
    Details
    ISSN: 1432-0584
    Keywords: Autoimmune thrombocytopenia ; Platelet glycoproteins ; Marrow transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report the case of a patient who developed autoimmune thrombocytopenia after allogeneic bone marrow transplantation from her HLA-identical sister. An IgG autoantibody was detected that bound to the platelet glycoprotein IIb/IIIa and to the HLA class I proteins. Immunoprecipitation studies with radiolabeled platelets revealed additional antibody binding sites on proteins of 214 kDa molecular weight under nonreduced conditions and 65 and 56 kDa molecular weight under reduced conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320436
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  • 7
    Electronic Resource
    Electronic Resource
    Detection of engraftment and mixed chimerism following bone marrow transplantation using PCR amplification of a highly variable region-variable number of tandem repeats (VNTR) in the von Willebrand factor gene (1991)
    Springer
    Annals of hematology 63 (1991), S. 227-228 
    Details
    ISSN: 1432-0584
    Keywords: Mixed chimerism ; PCR ; BMT ; Molecular genetics ; von Willebrand factor gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Detection of host cells in peripheral blood and/or bone marrow (mixed chimerism) of patients who have undergone bone marrow transplantation (BMT) is possible using either immunological methods or cytogenetic or molecular genetic analysis. We shall report a new method for the detection of mixed chimerism, which makes use of the fact, that the von Willebrand factor (vWF) gene has a highly variable region-variable number of tandem repeats (VNTR) — within intron 40. vWF-VNTR amplification by the polymerase chain reaction (PCR) was performed as described by Peake et al. [5]. We have studied 185 peripheral blood and/or bone marrow samples of 26 patients. Median time after BMT was 14 months (range 1–83 months). Of the 11 patients who were studied sequentially during the first 100 days following BMT, mixed chimerism was detected in four, but only transiently. None of these patients has relapsed so far. Of 18 patients who were studied more than 100 days after BMT mixed chimerism was found in three; two of these patients have subsequently relapsed. The advantages of this new method are: (a) it is informative in a high percentage of patients; (b) it requires only small amounts (200μl) of peripheral blood; (c) reliable results can be obtained at leukocyte counts of even less than 50 perμl. The clinical relevance and sensitivity of the method compared with established methods for detection of mixed chimerism remain to be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01703449
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  • 8
    Electronic Resource
    Electronic Resource
    Efficacy of the M-2 protocol in previously untreated patients with advanced multiple myeloma (1984)
    Springer
    Annals of hematology 49 (1984), S. 383-388 
    Details
    ISSN: 1432-0584
    Keywords: Multiple myeloma ; Combination chemotherapy ; Response rate ; Survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 37 consecutive, previously untreated patients with advanced multiple myeloma (16 patients Stage II, 21 patients Stage III) were treated with a five drug regimen consisting of carmustine, melphalan, vincristine, cyclophosphamide and prednisolone (M-2-protocol) in a prospective manner. Remission was achieved in 24 patients (65%). The median time to remission was 10 weeks, the median duration of remission 15,3 months. Median survival time from the onset of treatment was 24 months for all patients. Responding patients have a projected 65% three year survival. Median survival in non-responders was 10 months. 8 patients died during the first year of treatment. These results do not confirm the favourable results with this drug combination obtained in a previous trial. The discrepancy may be explained by a higher proportion of poor risk patients in the present study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319886
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  • 9
    Electronic Resource
    Electronic Resource
    Ratio of complement receptor over Fc-receptor III expression: A sensitive parameter to monitor granulocyte-macrophage colony-stimulating factor effects on neutrophils (1991)
    Springer
    Annals of hematology 62 (1991), S. 135-140 
    Details
    ISSN: 1432-0584
    Keywords: Granulocyte-macrophage colony-stimulating factor (GM-CSF) ; Granulocyte surface receptors ; Therapy monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In vitro activation of human granulocytes leads to altered expression of distinct surface antigens. Compared with the changes observed with classic activating reagents such as the phorbol ester PMA similar, but less pronounced alterations of surface antigen expression were observed upon granulocyte activation with human recombinant granulocyte-macrophage colony-stimulating factor (hrGM-CSF). In particular, stimulation with hrGM-CSF is followed by an enhanced expression of the complement receptors CD35 (CR1) and CD11b (CR3) while the low affinity Fc-γ receptor CD16 (FcRIII) is downregulated. In order to investigate whether there are similar effects under in vivo conditions, we studied the granulocytes from patients undergoing rhGM-CSF therapy before, during, and after treatment. We found a marked increase in CD35 (CR1) and CD11b (CR3) expression and a substantial decrease or even loss of CD16 (FcRIII) on these granulocytes. These changes correlated well with our in vitro data and occurred extremely rapidly after therapy onset. Furthermore, therapy monitoring using ratios calculated by the mean fluorescence channel numbers of CR and FcRIII stainings may combine the advantage of high sensitivity with high reproducibility as a result of the contrasting change in CR and FcRIII expression during granulocyte activation. Being nonparametric values, such ratios are not influenced by individual flow cytometry standardization. Taken together, these activation-associated changes of surface receptor expression and especially of CR over FcRIII ratios are useful parameters for monitoring the in vivo effects of rhGM-CSF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01702927
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  • 10
    Electronic Resource
    Electronic Resource
    Book reviews (1985)
    Springer
    Annals of hematology 51 (1985), S. 301-305 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320527
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