ISSN:
1432-1076
Keywords:
Lymphocyte
;
Phosphorylase kinase activity
;
Liver phosphorylase kinase deficiency
;
Sex-linked form
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Lymphocyte phosphorylase kinase activities were measured in normal controls and in patients with the sex-linked form of liver phosphorylase kinase deficiency. The reaction due to phosphorylase kinase activity in normal lymphocytes (2.7×106 in the reaction tube) was found to be linear within 20–60 min at 30° C. The reaction was directly proportional to the concentration of lymphocytes within 1.5×106–9.0×106, at 30° C for 60 min. The phosphorylase kinase activity in normal lymphocytes, which were pre-incubated at 50° C or 95° C for 1 min, decreased to 60% at 50° C and 10% at 95° C of that after pre-incubation at 0° C for 1 min. The activity of normal controls was 125±23.5 U/1010 lymphocytes. Those of the patients with liver phosphorylase kinase deficiency due to the sex-linked form were 43.5 U in case 1, 54.5 U in case 2, and 51.3 U in case 3, respectively and those of the mothers were within the normal range. These results suggest that phosphorylase kinase in lymphocytes might be form intermediate between liver and muscle phosphorylase kinase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00442132
Permalink