ISSN:
1573-0603
Keywords:
neurons
;
primary culture
;
collagen
;
fibronectin
;
laminin
;
heparan sulfate proteoglycan
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We used components of the extracellular matrix (ECM), which plays an important role in neuronal development, as coating materials for culture dishes. Components used were fibronectin (FN), laminin (LN), heparan sulfate proteoglycan (HSPG), type I collagen (COL-I), type IV collagen (COL-IV), and type VIII collagen (COL-VIII). We obtained highly neuron-enriched primary cultures from embryonic mouse whole brain by careful handling of materials, improvement of serum-free medium components, and reduction of seeding cell density. Neuronal cells were well differentiated with ECM (FN: 63.8%, LN: 76.2%, HSPG: 75.3%, COL-I: 63.3%, COL-IV: 55.4%, COL-VIII: 72.2%, respectively, on Day 5 in culture as assessed by determining percentages of neurite-bearing cells), whereas glial fibrillary acidic protein-positive cells remained minimized (FN: 3.6%, LN: 3.2%, HSPG: 2.8%, COL-I: 2.1%, COL-IV: 3.1%, respectively, on Day 5 in culture).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02388266
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