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  • 1
    ISSN: 1432-1238
    Keywords: Coma ; Benzodiazepines ; Flumazenil ; Liver failure ; Hepatic encephalopathy ; Pediatric
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective To evaluate the effect of flumazenil on hepatic encephalopathy complicating fulminant liver failure in children. Design Uncontrolled prospective study. Setting Pediatric Intensive Care Unit, tertiary care center. Patients 9 children with fulminant liver failure and hepatic encephalopathy awaiting emergency liver transplantation Interventions Changes in hepatic encephalopathy grade and in electroencephalogram were recorded during the injection of a bolus of flumazenil (0.01 mg/kg/IV bolus) followed by a continuous infusion of flumazenil (0.01mg/kg/h). Measurements and main results Before flumazenil, 7 children had grade 2 and 2 had grade 3 hepatic encephalopathy. Flumazenil injection mediated an arousal effect in 1 child in whom encephalopathy improved from grade 3 to grade 2. This effect lasted 30 min. No clinical response was observed in other children. An improvement of EEG anomalies was observed lasting 3 min in one child. Despite continuous infusion of flumazenil, encephalopathy worsened in all children. Conclusion The effect of intravenous administration of flumazenil on hepatic encephalopathy in children with fulminant liver failure is inconsistent. Its efficacy is transient. The therapeutic value of flumazenil in children with fulminant liver failure awaiting a liver graft is minimal at this dosage.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: Key words Aeroponic culture ; N2-fixation ; Acacia mangium ; Bradyrhizobium spp. ; Hypernodulation ; Tree saplings ; Imperata cylindrica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This work was designed to determine whether a plant culture method on non-solid media could be used as an alternative for inoculation of Acacia mangium with selected strains of Bradyrhizobium spp. A. mangium seedlings were grown and inoculated with Bradyrhizobium strain Aust13c and strain Tel2 in hydroponics, aeroponics and sand. Aeroponics was found to be the best system of the three, allowing the production of tree saplings 1 m in height after only 4 months in culture. Moreover, compared to plants grown in liquid or sand media, aeroponically grown saplings inoculated with Bradyrhizobium spp. developed a very high number of small nodules distributed all along the root system, resulting in an increase in nitrogen and chlorophyll content in plant tissues. We propose aeroponics as an alternative method to classical soil inoculation procedures for the production of hypernodulated legume tree saplings.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0789
    Keywords: Acacia mangium ; P fertilization ; Root nodulation ; N2 fixation ; Acetylene reduction assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant growth performance, the P content in root and nodule tissues, and nodulation and N2-fixing ability were studied in four provenances of Acacia mangium from Papua New Guinea following different levels of P fertilizer application. A. mangium did not seem to need high levels of P for growth and N2 fixation. The response by this leguminous tree to the P supply varied significantly according to provenance and to P concentrations in the culture solution. The provenances of A. mangium were classified into three types according to their P response: (1) Growth performance, nodulation, and N2 fixation of plants were stimulated as concentrations of P increased (provenance PH 482); (2) the maximal effect of P on plant growth was found only at P concentrations higher than 500 μM (provenance PH 484); and (3) the plant response to P fertilization was low, even with nutrient solutions containing P concentratins higher than 500 μM (provenances PH 483 and PH 485). Provenance PH 483 was distinguished by its low nodulating ability. However, this provenance grew well, probably because of its high N2 fixation efficiency as expressed by specific acetylene reduction activity and its high P content in nodule tissues. Therefore, in certain cases, these two parameters may be useful criteria in selecting leguminous plants for field use. Statistical analyses of the study results showed that the effect of the factor P supply on N2 fixation efficiency and nodule development was only significant at P concentrations lower than 250 μM whereas the effect of the factor plant provenance was significant regardless of the P concentration used. This observation emphasizes the value of provenance screening in the identification of plants for use in a wide range of soil types.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The SEMAPHORE (Structure des Echanges Mer-Atmosphère, Propriétés des Hétérogénéités Océaniques: Recherche Expérimentale) experiment has been conducted from June to November 1993 in the Northeast Atlantic between the Azores and Madeira. It was centered on the study of the mesoscale ocean circulation and air-sea interactions. The experimental investigation was achieved at the mesoscale using moorings, floats, and ship hydrological survey, and at a smaller scale by one dedicated ship, two instrumented aircraft, and surface drifting buoys, for one and a half month in October-November (IOP: intense observing period). Observations from meteorological operational satellites as well as spaceborne microwave sensors were used in complement. The main studies undertaken concern the mesoscale ocean, the upper ocean, the atmospheric boundary layer, and the sea surface, and first results are presented for the various topics. From data analysis and model simulations, the main characteristics of the ocean circulation were deduced, showing the close relationship between the Azores front meander and the occurrence of Mediterranean water lenses (meddies), and the shift between the Azores current frontal signature at the surface and within the thermocline. Using drifting buoys and ship data in the upper ocean, the gap between the scales of the atmospheric forcing and the oceanic variability was made evident. A 2 °C decrease and a 40-m deepening of the mixed layer were measured within the IOP, associated with a heating loss of about 100 W m−2. This evolution was shown to be strongly connected to the occurrence of storms at the beginning and the end of October. Above the surface, turbulent measurements from ship and aircraft were analyzed across the surface thermal front, showing a 30% difference in heat fluxes between both sides during a 4-day period, and the respective contributions of the wind and the surface temperature were evaluated. The classical momentum flux bulk parameterization was found to fail in low wind and unstable conditions. Finally, the sea surface was investigated using airborne and satellite radars and wave buoys. A wave model, operationally used, was found to get better results compared with radar and wave-buoy measurements, when initialized using an improved wind field, obtained by assimilating satellite and buoy wind data in a meteorological model. A detailed analysis of a 2-day period showed that the swell component, propagating from a far source area, is underestimated in the wave model. A data base has been created, containing all experimental measurements. It will allow us to pursue the interpretation of observations and to test model simulations in the ocean, at the surface and in the atmospheric boundary layer, and to investigate the ocean-atmosphere coupling at the local and mesoscales.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Annales geophysicae 15 (1997), S. 823-839 
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract This study presents an experimental analysis from aircraft measurements above the Pyrenees chain during the PYREX experiment. The Pyrenees chain, roughly WE oriented, is a major barrier for northerly and southerly airflows. We present a case of southerly flow (15 October 1990) and three successive cases of northerly flows above the Pyrenees (14, 15 and 16 November 1990) documented by two aircraft. The aircraft have described a vertical cross section perpendicular to the Pyrenean ridge. This area is described via the thermodynamical and dynamical fields which have a horizontal resolution of 10 km. Three methods for computing the vertical velocity of the air are presented. The horizontal advection terms which play a role in the budget equations are also evaluated. The altitude turbulence zone of 15 October are shown via turbulent fluxes, turbulent kinetic energy (TKE), dissipation rate of TKE and inertial length-scale. A comparison of results obtained by eddy-correlation and inertial-dissi-pation method is presented. The experimental results show a warm and dry downdraft for the southerly flow with large values for advection terms. All the mountain wave cases are also shown to present an important dynamical perturbation just above the Pyrenees at upper altitudes.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 173 (1999), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known increase in resistance of metacyclic and amastigote lifecycle forms to oxidant stress could be established. Amastigotes cultivated at 37°C also produced ovothiol A, as judged by incorporation of radiolabel from [3-methyl]methionine and [14C]histidine, and the incorporation of radiolabel from [35S]cysteine into ovothiol A represented about 10–15% of the total label recovered in ovothiol A, glutathione and trypanothione. Amastigotes were less susceptible than promastigotes to the effects of the redox cyclers paraquat and menadione and grew in culture in the presence of up to 20 mM buthionine sulfoximine, which completely blocked the synthesis of glutathione and its spermidine conjugates. Glutathione and trypanothione biosynthesis is, therefore, not necessary for the replication of L. donovani amastigotes in culture. Inhibition of the formation of glutathione and trypanothione did not result in an upregulation of ovothiol A production.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 850-852 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-203X
    Keywords: Key wordsM. truncatula ; M. falcata ; MsEnod12A ; MsEnod12B ; Srglb3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R108-1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all explants within 3–4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1420-9071
    Keywords: Key words. HCA; sequence analysis; helical net; protein folding.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Ten years after the idea of hydrophobic cluster analysis (HCA) was conceived and first pub lished, theoretical and practical experience has shown this unconventional method of protein sequence anal ysis to be particularly efficient and sensitive, especially with families of sequences sharing low levels of se quence identity. This extreme sensitivity has made it possible to predict the functions of genes whose se quence similarities are hardly if at all detectable by current one-dimensional (1D) methods alone, and of fers a new way to explore the enormous amount of data generated by genome sequencing. HCA also pro vides original tools to understand fundamental fea tures of protein stability and folding. Since the last review of HCA published in 1990 [1], significant im provements have been made and several new facets have been addressed. Here we wish to update and summarize this information.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-0867
    Keywords: soil system ; soil horizon ; building block ; mixing model ; digital elevation model ; TOPMODEL ; hillslope mechanistic model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The hydrology and water quality in landscapes with hydromorphic soils depends on the space and time extension of wetland areas and on water pathways within the landscape at different scales. To study the control of nitrate fluxes by these areas, investigations were carried out on a detailed study site - the Coët Dan catchment (1200 ha) in Brittany, France - involving various disciplines: pedology, soil physics, hydrology, geochemistry and agronomy. An attempt of functional modelling at different hierarchical levels from the horizon level (i - 1) to the region level (i + 3) of soil distribution, extension of saturated areas, horizons physical characteristics, water transfer in a multilayer soil profile and nitrate fluxes was carried out. The soil system, which can be described as a spatial arrangement of a limited number of horizon types with genetic relationships, is tightly controlled by topography. Predictive models of hydromorphic soil distribution using different topographic indexes and DEM were established. Regarding to their hydrodynamic properties, horizons of the soil system have been classified into “building blocks”, which allows to define physically based parameters for a two-dimensional multilayer water transfer model. A four compartment model of flood genesis based on chemical data obtained from different parts of the catena and from the river was coherent with the multilayer hydrodynamic model. The mean nitrate concentrations in several subcatchments were negatively correlated with the percentage of hydromorphic soils. These studies reveal that the hydromorphic zones had an effect on the nitrogen transfer in the catchment, but this effect is limited by the importance of water pathways by-passing the buffer zones. The conclusions of this programme have direct outcomes for designing new landscape management options.
    Type of Medium: Electronic Resource
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