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  • 1
    ISSN: 1432-055X
    Keywords: Schlüsselwörter EEG ; Anästhesie ; Monitoring ; Ableitungsauswahl ; automatische Narkosetiefebestimmung ; Key words EEG ; Anaesthesia ; Monitoring ; Selection of channels ; Automatic staging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract The conventional multichannel electroencephalogram is quite inconvenient for long-term monitoring in the operating theatre or intensive care unit. Recording of the EEG would be easier if a small number of channels was sufficient. Aiming at reduction of channels, leads from different regions of the scalp were analysed visually and with regard to their spectral content. Methods. Electrode placements corresponded to the International 10/20 System (Fig. 1). EEG recordings were made with a conventional device (ES 12000), a personal computer, and a spectral analyser. Two-channel recordings. Retrospective analysis was performed on data from 392 patients (age 14–90 years) whose anaesthesia was induced with various anaesthetics/narcotics, for instance thiopental, ketamine, etomidate, halothane, and enflurane. The EEG was recorded using C3–P3 and Cz–A1. For each patient the changes of spectral parameters during the course of the induction were plotted and visually analysed. For statistical analyses a 30-s epoch of each patient was randomly selected from the first few minutes after the beginning of induction. Ten-channel recordings. In ten gynaecological patients (age 26–55 years) EEG recordings were performed during induction of anaesthesia with thiopental in combination with fentanyl, N2O and O2. The set of channels consisted of Fz′–Cb1, F3′–Cb1, Cz–Cb1, C3–Cb1, P3–Cb1, Oz–Cb1, Fz′–F3′, F3′–C3, C3–P3, and P3–Oz. The electrodes Fz′ and F3′ were positioned on the forehead near to Fz and F3, respectively. These sites were chosen because they allow easy application of electrodes. The relationship between channels was calculated with Bravais-Pearson's coefficient of correlation for the power and the absolute power in the frequency bands delta (0.5–3.5 Hz), theta (3.5–7.5 Hz), alpha (7.5–12.5 Hz), and beta (〉12.5 Hz). Results. In visual and statistical analyses of the two- and ten-channel recordings under the influence of anaesthetics/narcotics, similar changes of EEG activity could be observed in all channels. Although differences in the absolute power of the frequency bands were present, there was high conformity in the composition of the spectral content of the different channels. Classification of the EEG into stages of anaesthesia by means of a single channel led to consistent results for all channels. Alpha activity as leading feature of the awake state predominated occipitally. In channels including the region around the ears, contamination with EKG artifacts was observed. Conclusions. EEG patterns under the influence of different anaesthetics/narcotics are adequately represented by a reduced number of channels. For the choice of an appropriate set of channels the following aspects should be considered. Contamination with artifacts should be as low as possible, electrode sites should easily be accessible, and special features of the awake state should be identifiable. Experience with routinely conducted EEG recordings in the operating theatre and the intensive care unit showed that the channels C3–P3 or C4–P4 provide a sufficient basis for automatic staging of the depth of anaesthesia, which is implemented in the EEG monitor Narkograph.
    Notes: Zusammenfassung EEG-Registrierungen mit einer größeren Anzahl von Ableitungen sind technisch aufwendig. Als Routinemonitoringverfahren z.B. zur Beurteilung der Effekte von Anästhetika/Narkotika bei der Steuerung der Narkose sind sie daher wenig geeignet. Die EEG-Ableitung wäre einfacher, wenn sie mit wenigen Kanälen erfolgen könnte. Daher sollten in dieser Arbeit EEG-Ableitungen von unterschiedlichen Regionen des Kopfs mit dem Ziel einer Kanalreduktion visuell und im Hinblick auf den spektralen Gehalt verglichen werden. Dazu wurden 392 EEG-Kurven mit zwei Kanälen aus Narkoseeinleitungen mit unterschiedlichen Anästhetika/Narkotika und zehn EEG-Aufzeichnungen mit jeweils zehn Kanälen bei Narkoseeinleitungen mit Fentanyl und Thiopental untersucht. Die visuellen und statistischen Auswertungen zeigen, daß unter dem Einfluß von Anästhetika/Narkotika in unterschiedlichen Ableitungen gleichartige EEG-Bilder auftreten und anhand einer einzelnen dieser Ableitungen eine Stadienklassifikation möglich ist. Bei eigenen Routinemessungen haben sich die Ableitungen C 3 −P 3 oder C 4 −P 4 als Grundlage für ein EEG-Monitoring in der Anästhesie als geeignet erwiesen. Diese Ableitungen bieten neben leichter Zugänglichkeit der Ableiteelektroden gute Voraussetzungen für die Erkennung des Wach-EEG und sind im Vergleich mit anderen Ableitungen artefaktarm.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1440
    Keywords: Key Words Pancreas ; Islets of Langerhans ; Islet isolation ; Pancreas morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In clinical islet transplantation to patients with type 1 diabetes mellitus, the number of isolated and purified islet has been identified as a key determinant for functional success of the islet graft [1]. With improved isolation methods based on the original procedure published by Ricordi et al. [2] yield and function of isolated islets were considerably enhanced. However, there is still a large variance in the number, purity, viability and secretory capacity of islets isolated from brain-dead human donor pancreata, significantly hampering utilization of human islet preparations derived from a single donor for one diabetic recipient. The reasons for the limited success in islet isolation and purification have not been clarified in detail yet. Recent studies have indicated, that donor preconditions, and a number of technical factors during organ procurement and the islet isolation process itself are critical to successful islet isolation [3, 4]. This study aimed at identifying distinct morphological and histopathological characteristics of the donor pancreas as determinants for the outcome of human islet isolation and purification.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0649
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Using a confocal microscope with a single-photon avalanche photodiode as detector, we studied photon bursts of single Rhodamine 6G (R6G) and Rhodamin B-zwitterion (RB) molecules in aqueous solution by excitation of the lowest excited singlet stateS 1 with a frequency-doubled titanium: sapphire laser. Multichannel scaler traces, the fluorescence autocorrelation function and fluorescence decay times determined by time-correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. Fluorescence lifetime patterns in steps of 100 ps were generated by convolution with the excitation pulse. The lifetime of theS 1 state was derived from the Kullback-Leibler minimum discrimination information. We are able to demonstrate for the first time identification of two different single dye molecules via their characteristic fluorescence lifetimes of 1.79 ± 0.33 ns (RB) and 3.79 ± 0.38 ns (R6G) in aqueous solution.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1076
    Keywords: Key words Antithrombin III ; Coagulation ; Haemangiomatosis ; Kasabach-Merrit syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Diffuse infantile haemangiomatosis of the spleen is a very rare lesion. Large haemangiomas may cause trapping of platelets and coagulation disorders known as Kasabach-Merrit syndrome. We here report the case of an infant with splenic and pancreatic haemangiomatosis presenting with life-threatening thrombocytopenia, anaemia and intravascular coagulation. Diagnosis was hampered by reactive erythroblastosis and non-conclusive radiological findings. While treatment with corticosteroids was ineffective, administration of antithrombin III improved coagulation parameters. After splenectomy the child recovered promptly and has remained free of disease for 3 years to date. Conclusion Occult visceral haemangiomatosis without visible cutaneous haemangiomas should be included in the differential diagnosis of thrombocytopenia, anaemia and consumption coagulopathy. Antithrombin III treatment may be considered to overcome bleeding problems in patients with Kasabach-Merrit syndrome.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 77 (1995), S. 4973-4978 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The x-ray structure (λ〈10 A(ring)) of the DPF-78 discharge was investigated with different heavy-gas admixtures to a basic D2 filling. A possible classification of plasma features is proposed. Micropinches with an unusual high aspect ratio of about 10:1 were found. Soft x-ray spectra of single micropinches were registered on film. The use of x-ray spectroscopic methods by means of spectra simulation codes allowed us to estimate the parameters of particular types of pinches with axial size of 0.5 mm and full radial size of 0.05 mm. In the He-like ionization state we obtained ne≈3×1021 cm−3 and Te≈0.8 keV. The electron temperature determined in the H-like ionization stage was considerably higher, Te≈2 keV, indicating a strong ionizing plasma. Possible deviations from the Bennett condition are discussed in connection with the spectroscopic results. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental dermatology 21 (1996), S. 0 
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Temporal arteritis, a variant of giant cell arteritis, is a systemic granulomatous vasculitis of large and medium-sized arteries. Usually the clinical Features arc dominated by ophthalmological and neurological complications. In rare instances, ischaemic necrosis, especially of the scalp, may lead patients to the dermatologist. We report a 76-year-old woman presenting with a unilateral scalp necrosis, accompanied by a dramatic ipsilateral impairment of vision. Immediately after duplex-sonography of the extracranial vessels and after initiation of corticosteroid therapy, the diagnosis of temporal arteritis was confirmed by temporal art en biopsy. One month later, because of insufficient secondary healing of the ulcer, the defect was covered by a mesh graft. The taking of the graft was delayed due to immunosuppressive therapy, but was complete. The patient unfortunately died as a result of complications related to surgical removal of an aspergilloma in the sphenoid cavity secondary to immunosuppressive therapy. We discuss the technique of artery biopsy and the possibility of surgical management of scalp necrosis in temporal arteritis.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 19 (1996), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In many plants, translocation of sucrose from mesnsophyll to phloem for long-distance transport is carrier-mediated. The sucrose H+-symporter gene SUT1 from potato is expressed at high levels in the phloem of mature, exporting leaves and at lower levels in other organs. Inhibition of SUT1 by expression of an antisense gene in companion cells under control of the rolC promoter leads to accumulation of high amounts of soluble and insoluble carbohydrates in leaves and inhibition of photosynthesis. The distribution of in situ localized starch does not correspond with areas of reduced photosynthesis as shown by fluorescence imaging. Dissection of antisense effects on sink and source organs by reciprocal grafts shows that inhibition of transporter gene expression in leaves is sufficient to produce chlorosis in leaves and reduced tuber yield. In contrast to the arrest of plasmodesmal development found in plants that express yeast invertase in the apoplast, in mature leaves of sucrose transporter antisense plants plasmodesmata are branched and have median cavities. These data strongly support an apoplastic mode of phloem loading in potato, in which the sucrose transporter located at the plasma membrane of the sieve element/companion cell complex represents the primary route for sugar uptake into the long-distance translocation pathway.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1434-9957
    Keywords: Surgery planning ; Volume segmentation ; Virtual tools ; Force feedback ; Intra-operative navigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract The primary goal of our research has been to implement an entirely computer-based maxillofacial surgery planning system [1]. An important step toward this goal is to make virtual tools available to the surgeon in order to carry out a three-dimensional (3D) cephalometrical analysis and to interactively define bone segments from skull and jaw bones. An easy-to-handle user interface employs visual and force-feedback devices to define subvolumes of a patient's volume dataset [2]. The defined subvolumes, together with their spatial arrangements based on the cephalometrical results, eventually lead to an operation plan. We have evaluated modern low-cost, force-feedback devices with regard to their ability to emulate the surgeon's working procedure. Once the planning of the procedure is complete, the planning results are transferred to the operating room. In our intra-operative concept the visualisation of planning data is speech controlled by the surgeon and correlated with the patient's position by an electromagnetic 3D sensor system.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Intensivmedizin und Notfallmedizin 36 (1999), S. 156-162 
    ISSN: 1435-1420
    Keywords: Key words Noninvasive mechanical ventilation ; acute respiratory failure ; intensive care unit ; nasal ventilation ; Schlüsselwörter Nichtinvasive Beatmung ; Maskenbeatmung ; akute respiratorische Insuffizienz ; Intensivmedizin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die nichtinvasive Beatmung über eine Maske ermöglicht bei vielen Patienten eine effiziente Beatmung unter Vermeidung der Risiken und Nebenwirkungen der endotrachealen Intubation. Für die chronisch-obstruktive Lungenerkrankung sind die Vorteile der Maskenbeatmung im Hinblick auf Beatmungsdauer, Dauer der Intensivbehandlung, Prognose und Behandlungskosten inzwischen relativ gut durch Studien belegt. Bei anderen Indikationen erlauben die verfügbaren Daten noch kein gesichertes Urteil, rechtfertigen aber einen Behandlungsversuch auch außerhalb von Studien in der täglichen klinischen Routine. Nachteilig sind der tendenziell höhere personelle Betreuungsaufwand bei der nichtinvasiven Beatmung sowie die starke Erfahrungsabhängigkeit der Ergebnisse. Die wichtigste Voraussetzung für eine erfolgreiche nichtinvasive Beatmung in der Intensivmedizin ist die Bereitschaft von Ärzten und Pflegepersonal, eine ungewohnte Beatmungsphilosophie zu akzeptieren und die relativ einfache Technik der Maskenbeatmung zu erlernen.
    Notes: Summary Noninvasive positive pressure ventilation provides effective ventilatory support for many patients with acute respiratory failure and avoids the risks and side effects of endotracheal intubation. Controlled clinical studies demonstrate improved outcome and cost effectiveness for patients with acute exacerbations of chronic obstructive pulmonary disease. The data on use of noninvasive ventilation for other forms of acute respiratory failure are less clear, but justify a consideration of mask ventilation in many clinical situations. Noninvasive ventilation tends to be more time-consuming and more dependent on experienced ICU staff than conventional mechanical ventilation. It is essential for the success of this “new” therapy that physicians and nursing staff try to become familiar not only with the technique but also with a different approach to mechanical ventilation.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Intensivmedizin und Notfallmedizin 34 (1997), S. 466-471 
    ISSN: 1435-1420
    Keywords: Key words Procalcitonin ; C-reactive protein ; sepsis ; acute phase response ; Schlüsselwörter Procalcitonin ; Akute-Phase-Reaktion ; CRP ; Sepsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In dieser Übersichtsarbeit wird eine kritische Bewertung der zur Verfügung stehenden infektiologischen Laborparameter vorgenommen. Dies geschieht unter besonderer Berücksichtigung der intensivmedizinischen Situation. Die bisher etablierten infektiologischen Parameter (BSG, BB, CRP) werden dem neuen ,,Sepsis-Marker`` Procalcitonin (PCT) gegenüber gestellt. Zum jetzigen Zeitpunkt läßt sich die zukünftige Rolle des PCT noch nicht abschließend einschätzen. Möglicherweise bestehen jedoch wesentliche diagnostische Vorteile bei leberinsuffizienten, neutropenen und anderweitig immunkompromitierten (transplantierten) Patienten.
    Notes: Summary Diagnosis of bacterial infection and/or sepsis in critically ill patients is a common problem in intensive care medicine. White blood count, C-reactive protein (CRP), and the erythrocyte sedimentation rate (ESR) are often used in the differential diagnosis of infection versus SIRS (systemic inflammatory response syndrome) due to noninfectious causes. There is a number of non-infectious stimuli to cause leucocytosis and CRP elevation. In the last 3 years, Procalcitonin (PCT) was determined to be a marker of severe bacterial infection, especially sepsis. PCT appears to have several advantages; it is of non-hepatic (yet unknown) origin and useful in patients with hepatic insufficiency. There is no PCT-increase in acute graft-rejection, viral disease, and autoimmune disorders. Some studys showed a prognostic value of PCT in sepsis due to peritonitis and pancreatitis. PCT appears to be a new and helpful tool in the diagnosis and treatment of critically ill patients. Further studies are needed to finally established PCT in the daily management of critically ill patients. At this time significantly higher costs are the major detraction to daily or frequent measurements.
    Type of Medium: Electronic Resource
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