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  • 1
    Electronic Resource
    Electronic Resource
    Anti-HBc IgM bei akuter und chronischer Hepatitis-B-Virus-Infektion (1984)
    Springer
    Journal of molecular medicine 62 (1984), S. 837-842 
    Details
    ISSN: 1432-1440
    Keywords: Hepatitis B virus ; Hepatitis markers ; Anti-hepatitis B core immunoglobulin M
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Hepatitis B core antigen (HBcAg) synthesized in E. coli was used for determination of immunoglobulin M class-specific antibodies against HBcAg. It was found that 98% of cases with acute hepatitis B surface antigen (HBsAg) positive hepatitis type B were anti-HBc immunoglobulin M (IgM) positive. Atypical hepatitis B was detected in 33% of anti-HBc-positive HBsAg-negative cases with acute hepatitis. Anti-HBc IgM was positive for 6 months in acute resolving hepatitis type B, whereas cases resulting in chronic hepatitis B remained anti-HBc IgM-positive for up to 900 days. Chronic HBsAg carriers with severe liver disease had anti-HBc IgM more often than individuals with minor liver damage; 83% of HBsAg-positive liver cirrhoses, 63% of chronic aggressive hepatitis, 50% of HBsAg-positive liver carcinoma, but only 17% of chronic persistent hepatitis or 7% of healthy blood donors were anti-HBc IgM-positive. Determination of anti-HBc IgM is useful in detecting atypical hepatitis B virus infections without HBsAg in serum and, with some restrictions, in discriminating acute and chronic hepatitis type B.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01711864
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella andTreponema pallidum (1982)
    Springer
    European journal of clinical microbiology & infectious diseases 1 (1982), S. 118-121 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A rapid and easy method for isolating IgM from serum specimens in order to detect specific antibodies againstTreponema pallidum and rubella virus by routine serologic procedures is described. Serum IgM was isolated by immunoaffinity chromatography using anti-human IgM antibodies covalently bound to controlled-pore glass beads in a microcolumn. The final concentration of the IgM in the samples tested amounted to at least 16 % (average 32 %) of the original concentration (corresponding to a serum dilution of 1∶〈8). IgG contamination did not exceed 0.38 % of the original serum concentration. The capacity of the column was stable for at least 50 absorption/ elution cycles. The new technique enables rapid and reliable detection of specific IgM by the rubella hemagglutination inhibition andTreponema pallidum hemagglutination tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02014203
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Use of hepatitis B core antigen produced inEscherichia coli to detect immunoglobulin M specific antibodies in an enzyme-linked immunosorbent assay (1984)
    Springer
    European journal of clinical microbiology & infectious diseases 3 (1984), S. 30-34 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The antigenic activity of HBcAg produced inEschericha coli and HBcAg from human liver was compared in aμ-specific solid-phase antibody-capture assay for detection of anti-HBc-IgM. HBcAg from liver could be detected in dilutions up to 1∶3, HBcAg fromEscherichia coli in dilutions up to 1∶10, 000. Using HBcAg fromEschericha coli, sera from five patients with acute resolving hepatitis B and sera from four patients with actue hepatitis B who had developed chronic liver disease were tested for anti-HBc-IgM in ELISA. IgM fractions separated out of the same sera by immunoaffinity chromatography were tested for anti-HBc-IgM using a commercially available test. The results were in good agreement with those obtained by ELISA. Anti-HBc-IgM could be detected up to 900 days after onset of disease. Different groups of patients were tested for presence of anti-HBc-IgM in ELISA. Fifty-nine of 60 patients with acute hepatitis B were positive for anti-HBc-IgM at onset of illness. Ten of 16 patients with chronic aggressive hepatitis and seven of 23 HBsAg positive dialysis patients were also positive for anti-HBc-IgM, whereas only two of 12 patients with chronic persistent hepatitis and one of 15 HBsAg positive blood donors (“healthy” carriers of HBsAg) had detectable anti-HBc-IgM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02032811
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    Paper (German National Licenses)
    Fulltext
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