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  • 1
    Electronic Resource
    Electronic Resource
    Presence of calmodulin in human platelet cytoskeletons and its concentration change upon activation of platelets
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 801 (1984), S. 290-297 
    Details
    ISSN: 0304-4165
    Keywords: (Human) ; Calmodulin ; Cytoskeleton ; Platelet activation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(84)90079-5
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid effects ofphorbol ester on platelet shape change, cytoskeleton and calcium transient
    Amsterdam : Elsevier
    FEBS Letters 206 (1986), S. 273-278 
    Details
    ISSN: 0014-5793
    Keywords: (Blood platelet) ; Ca^2^+ transient ; Cytoskeleton ; Myosin ; Phorbol ester ; Protein kinase C
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(86)80995-4
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  • 3
    Electronic Resource
    Electronic Resource
    Mersalyl, a sulfhydryl reagent, alters the solubility of myosin and cytoskeletal proteins of human platelets
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 880 (1986), S. 197-203 
    Details
    ISSN: 0304-4165
    Keywords: (Human platelet) ; Cytoskeleton ; Mersalyl ; Myosin ; Sulfhydryl
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(86)90080-2
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of rat faecal metabolites of ebastine by B/E linked scanning liquid secondary ion mass spectrometry (1994)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 23 (1994), S. 385-390 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The identification of rat faecal metabolites of a new antihistaminic agent, ebastine, 4′-tert-butyl-4-[4-(diphenylmethoxy)piperidino]butyrophenone, is presented. After oral administration of (14C)ebastine (20 mg kg-1) to rats, 84% of the radioactive dose was excreted in the 24 h faeces. Unchanged drug and five metabolites were isolated from the faeces by thin-layer chromatography and solid-phase extraction, and their structures were identified by liquid secondary ion mass spectrometry using the B/E linked scanning technique. The main metabolic pathways were oxidation of a terminal methyl group to give the hydroxymethyl and carboxyl derivatives, and hydroxylation of a phenyl ring in the diphenylmethoxy moiety. In addition to the oxidative mechanism, metabolism of ebastine involved sulphate conjugation. It is noteworthy that M-4, having both phenolic and alcoholic hydroxyl groups, was sulphated selectively in the latter position.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200230702
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  • 5
    Electronic Resource
    Electronic Resource
    Fractography of nylon-6 yarn (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 24 (1979), S. 837-852 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The breaking mechanism of nylon-6 yarn is investigated, and the breakage of the yarn under simple tensile loading conditions is compared to that of a filament caused by the same loading. The simple model yarn consisting of seven filaments was prepared for this purpose. The yarn was ruptured at a low rate of extension, and the fracture morphologies of the filaments in the yarn were inspected by a scanning electron microscopy. These morphologies are clearly different from those of the filaments broken individually under the same testing conditions used for the yarn. Various kinds of appearances are obtained in the yarn, for example, a large V-shaped notch is observed, the controlled ductile tearing region is developed across the filament at a large angle to its axis, and the ruptured end is expanded into a plane which is perpendicular to its axis. These causes are discussed in detail to understand the breaking mechanism of the yarn.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1979.070240319
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of the addition of a liquid crystalline copolyester to polystyrenes on blending torque and mechanical properties of blends (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 48 (1993), S. 383-391 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Blending of polystyrenes (PS) with a thermotropic liquid crystalline polymer (LCP) was performed by using a continuous corotating twin screw extruder. The influence of LCP content on the blending process was studied by changing the barrel heater temperature and the screw speed. The torque of screw shafts, generated during the blending process, was influenced by LCP content and its influence was not simple. The torque generated during the blending process was not directly related to the apparent melt viscosity of blends. Further, the effects of the matrix viscosity on the morphology and mechanical properties of the PS/LCP blends were studied using three grades of PS as matrix resins. It was found that the size of the LCP dispersed phase decreased with increasing matrix viscosity. Consequently, the mechanical properties of the PS/LCP blend were improved. © 1993 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1993.070480302
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  • 7
    Electronic Resource
    Electronic Resource
    Development of a new advanced process for manufacturing polyacetal resins. Part III. End-capping during polymerization for manufacturing acetal homopolymer and copolymer (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 50 (1993), S. 1317-1329 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Conventionally, acetal homopolymer or copolymer is obtained by the polymerization of formaldehyde or trioxane, following the end-capping using acetic anhydride or unzipping of the unstable polymer end fraction. First, Asahi Chemical developed a new process to obtain an end-capped polymer during polymerization of highly purified formaldehyde using acetic anhydride as the chain-transfer agent. Use of highly purified formaldehyde and endcapping during polymerization using acetic anhydride as a chain-transfer agent or an endcapping agent will provide a simple process for manufacturing acetal homopolymer. The polymerization mechanism was confirmed by infrared spectroscopy analysis and proton NMR analysis of the polymer obtained. Second, for the acetal copolymer, purified trioxane was copolymerized with ethylene oxide in the presence of methylal, which gave an endcapped polymer with high thermal stability. Two new intermediates from the initiation reaction of the copolymerization, 1,3,5,7-tetraoxacyclononane (TOCN) and 1,3,5,7,10-pentaoxacyclododecane (POCD), were isolated and a new initiation mechanism was proposed. © 1993 John Wiley & Sons, Inc.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1993.070500803
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  • 8
    Electronic Resource
    Electronic Resource
    Possible involvement of membrane fluidity in helicoidal microfibrillar orientation in the coenocytic green alga,Boergesenia forbesii (1994)
    Springer
    Protoplasma 180 (1994), S. 82-91 
    Details
    ISSN: 1615-6102
    Keywords: Boergesenia forbesii ; Cellulose synthesizing complex ; Cytoskeleton ; Helicoidal wall ; Membrane fluidity ; Microfibril orientation ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Microfibrillar textures and orientation of cellulose microfibrils (MFs) in the coenocytic green alga,Boergesenia forbesii, were investigated by fluorescence and electron microscopy. Newly formed aplanosporic spherical cells inBoergesenia start to form cellulose MFs on their surfaces after 2 h of culture at 25°C. Microfibrillar orientation becomes random, fountain-shaped, and helicoidal after 2, 4, and 5 h, respectively. The fountain orientation of MFs is usually apparent prior to helicoidal MF orientation and thus may be considered to initiate helicoid formation. Microfibrils continue to take on the helicoidal arrangement during the growth ofBoergesenia thallus. The helicoidal orientation of MFs occurs through gradual counterclockwise change in MF deposition by terminal complexes (TCs) viewed from inside the cell. On the dorsal side of curving TC impressions in helicoidal texture formation on a freeze-fractured plasma membrane, the aggregation of intramembranous particles (IMPs) occurs. Membrane flow may thus possibly affect the regulation of helicoidal orientation inBoergesenia. Following treatment with 3 μM amiprophos-methyl (APM) or 1 mM colchicine, cortical microtubules (MTs) completely disappear within 24 h but helicoidal textures formation is not affected. With 15 μM cytochalasin B or 30 μM phalloidin, however, the helicoidal orientation of MFs becomes random. Treatment with CaCl2 (10 mM) causes the helicoidal MF orientation of cells to become random, but co-treatment with N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide (W-7) (100 mM) prevents this effect, though W-7 has no effect on the helicoidal MF formation. It thus follows that MF orientation inBoergesenia possibly involves actin whose action may be regulated by calmodulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01379226
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative study of various methods for thermogravimetric analysis of polystyrene degradation (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 25 (1980), S. 2869-2877 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Various methods of thermogravimetric analysis were compared in case of polystyrene degradation. The reaction order, the activation energy and the preexponential factor were evaluated from common TG curves experimentally obtained. In most cases a 5-mg sample of polystyrene with 110,000 molecular weight was decomposed at heating rate of 10°C/min. The reaction was found under this experimental condition to be of first order with activation energy of 61.0 kcal/mole, though there were slight differences depending on the analytical methods used.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1980.070251218
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  • 10
    Electronic Resource
    Electronic Resource
    Metabolic engineering of cultured tobacco cells (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 329-332 
    Details
    ISSN: 0006-3592
    Keywords: tobacco cultured cells ; heat-shock promoter of Arabidopsis thaliana ; strong promoter from tobacco cell ; β-glucuronidase ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Construction of a gene expression system in tobacco cultured cells (BY2) was studied. A 925 bp promoter fragment of a heat-shock protein gene (HSP18.2) of Arabidopsis thaliana showed clear heat-shock response of expression of the β-glucuronidase (GUS) reporter gene in BY2 cells. Similar results were observed in a 500 mL flask and 3-L jar fermentor.Isolation of strong promoters in BY2 cells was tried. cDNA clones, in which the mRNA level is high in log-phase cells and the copy number in the genome is low, were isolated. These clones showed high homology with F1-ATPase (mitochondria type), elongation factor 1-α, and a gene with an unknown function of A. thaliana (clone 27), respectively. A 5′-flanking region of clone 27 showed 6.2 times the promoter activity of the CaMV35S promoter in BY2 cells.Three cDNA clones, which are expressed in the stationary growth phase of BY2 cells, were isolated by a differential screening. These clones showed high sequence homologies to alcohol dehydrogenase, pectin esterase, and extensin. Promoters of these genes will be useful in gene expression in high cell-density culture. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:329-332, 1998.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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