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  • 1
    ISSN: 1432-0428
    Keywords: Calcium ; EDTA ; glucose tolerance ; insulin ; uraemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of calcium infusion on the intravenous glucose tolerance was tested in hypocalcaemic patients suffering from renal insufficiency. It was shown that the delayed glucose disappearance rate in uraemics could be improved (p 〈 0.005) by the infusion of calcium gluconolactobionate despite the fact that the serum concentrations of potassium, urea nitrogen, and the blood pH were not altered. The basal insulin concentration was significantly depressed by the calcium infusion (p 〈 0.02). The serum calcium concentration was significantly correlated to the glucose assimilation coefficient in the uraemic patients (p 〈 0.01). — 3 hypocalcaemic patients without renal failure had a normal glucose tolerance and a normalization of the serum calcium concentration had no discernable effect. — A slight but significant decrease of the serum calcium concentration (p 〈 0.01) by EDTA-Na2 in normocalcaemic patients did not change the intravenous glucose tolerance. — It is concluded that hypocalcaemia may be one of the causes for the abnormal glucose tolerance in chronic renal failure.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Key words ; Glucose ; fructose ; insulin ; isolated fat cells ; re-esterification of free fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In isolated human fat cells of the greater omentum and the mammary gland, the effect of glucose, fructose, and/or insulin was tested on the re-esterification rate of FFA measured by the balance method. It could be shown that in the absence of glucose no re-esterification activity was demonstrable. Glucose alone or fructose alone stimulated the re-esterification of FFA dose-dependently in isolated fat cells of the greater omentum, and to a minor degree in fat cells of the mammary gland. Insulin had no effect on the re-esterification rate of FFA in the presence or absence of glucose or fructose, whereas it significantly stimulated the incorporation of glucose-C into CO2 and lipids. It is concluded that the re-esterification of FFA in human adipose tissue, at least in vitro, is mainly controlled by glucose without need for insulin.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1440
    Keywords: Lipoproteidlipase ; Lipoproteide ; Hyperlipoproteinämie Typ V ; Lipoprotein lipase ; Lipoproteins ; Type V Hyperlipoproteinemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The effects of the main lipoprotein density classes on the human adipose tissue lipoprotein lipase activity were studied. A dose-dependent stimulation of lipoprotein lipase activity was obtained for HDL and, to a lesser extent, for VLDL on a constant weight basis. LDL exerted virtually no effect. At higher concentrations, HDL as well as VLDL inhibited the stimulated lipolytic activity. In type V hyperlipoproteinemia, the stimulating effect of VLDL and of HDL was significantly lower, whereas the inhibiting action of HDL was markedly increased.
    Notes: Zusammenfassung Die Effekte der einzelnen Lipoproteidfraktionen des Plasmas auf die Aktivität der Lipoproteidlipase des menschlichen Fettgewebes wurden untersucht. HDL (high density lipoproteins) und, in geringerem Ausmaß, VLDL (very low density lipoproteins) bewirkten eine dosisabhängige Stimulierung der Aktivität der Lipoproteidlipase. LDL (low density lipoproteins) übten praktisch keinen Effekt aus. In höheren Konzentrationen hemmten sowohl HDL als auch VLDL die stimulierte lipolytische Aktivität. Bei Patienten mit Hyperlipoproteinämie Typ V war der stimulierende Effekt der VLDL und HDL viel geringer, die Hemmwirkung der HDL jedoch deutlich stärker ausgeprägt.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 62 (1984), S. 593-594 
    ISSN: 1432-1440
    Keywords: Lipoprotein lipase ; Blood-pH in vivo ; Acidosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Diseases associated with acidotic blood-pH, such as chronic renal disease, diabetes mellitus or chronic alcoholism, show a marked impairment of lipoprotein lipase. Therefore we influenced blood-pH in 3 healthy subjects by infusions to get alkalotic, neutral and acidotic blood-pH on three days in series. On each day blood-pH from capillary blood and post-heparin lipoprotein lipase from fasting plasma was determined. In comparison to neutral blood-pH in vivo, alkalosis did not influence lipoprotein lipase. In contrast, during artificial acidosis, lipoprotein lipase was impaired significantly (p〈0.01). Therefore, it seems, that acidosis inhibits lipoprotein lipase in vivo.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 170 (1977), S. 109-114 
    ISSN: 1433-8580
    Keywords: Adipose tissue ; Lipolysis ; Serum stimulation ; Lipoprotein lipase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An assay of lipolytic activity in human adipose tissue is described, in which native homogenates of the adipose tissue yield the enzyme, as well as the triglyceride substrate, and the emulsifying phospholipids. The lipolytic activity in the presence of serum is characterized mainly as lipoprotein lipase activity by a pH-optimum of 8.0, by the fact that serum is necessary for full activity, and that it is inhibited by 1 M NaCl and by protamine. At serum concentrations of higher than 50% a marked inhibition of the lipolysis is observed. Noradrenaline, insulin, and heparin have no effect on the serum-stimulated lipolytic activity.
    Type of Medium: Electronic Resource
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