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  • 1
    Digitale Medien
    Digitale Medien
    Tissue distribution and developmental profiles of immunoreactive αB crystallin in the rat determined with a sensitive immunoassay system
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 1074 (1991), S. 201-208 
    Details
    ISSN: 0304-4165
    Schlagwort(e): Crystallin ; Immunoassay ; Immunohistochemistry
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0304-4165(91)90062-L
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  • 2
    Digitale Medien
    Digitale Medien
    The expression of placental-type glutathione S-tranferase (GST-π) in human cutaneous squamous cell carcinoma and normal human skin (1995)
    Springer
    Virchows Archiv 425 (1995), S. 589-592 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Placental-type glutathione S-transferase ; Human skin ; Squamous cell carcinoma ; Northern blotting ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The expression of human placental-type glutathione S-transferase (GST-π) was investigated in human cutaneous squamous cell carcinoma (SCC) and normal skin using Northern blot and immunohistochemical analysis. In Northern blot examination, the expression of GST-π transcript was recognized in all instances, and SCC showed a significantly higher expression of GST-π than normal skin. In immunohistochemical examination, GST-π was stained well in the cytoplasm of all cells of the stratum granulosum, many cells of the stratum spinosum and a few cells of the stratum basale in normal skin. Some cells of the stratum spinosum and almost all cells of the stratum basale showed only a weakly positive or almost negative reaction for GST-π. No nuclear staining of GST-π was obvious in normal epidermal cells. In SCC, many cells showed strong positivity for GST-π in the cytoplasm, and some were obviously accompanied by nuclear staining of GST-π. These findings suggest that GST-π exists mainly in many cells in the upper layers of the normal epidermis and that GST-π is involved in the process of carcinogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00199348
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  • 3
    Digitale Medien
    Digitale Medien
    Immunohistochemical localization of lipocortins in normal and psoriatic human skin (1993)
    Springer
    Archives of dermatological research 285 (1993), S. 296-299 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Immunoblotting ; Immunohistochemistry ; Lipocortin ; Psoriasis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The distribution of lipocortin I, a steroid-induced inhibitory protein of phospholipase A2, was examined in normal and psoriatic human skin. Using immunoblotting analysis with specific antibody against human lipocortin I purified from human placenta, lipocortin I was detected as a 37 kDa protein in cultured epidermal cells, whole skin and epidermis. In the dermis and stratum corneum, lipocortin I was only weakly detectable by Western blotting. In contrast to normal skin, much less lipocortin I was detected by Western blotting analysis in psoriatic skin. Using immunoperoxidase immunohistochemical analysis, lipocortin I was demonstrated in the cytoplasm of keratinocytes in the upper and middle layers of the epidermis and in some infiltrating cells in the dermis in normal skin. In involved psoriatic skin, by contrast, lipocortin I was almost undetectable in the epidermis, although it was demonstrated in some infiltrating cells in the dermis. No immunostaining of lipocortin I was observed in the stratum corneum of normal or psoriatic skin. These results, together with the finding that phospholipase A2 activity is higher in psoriatic epidermis than in normal epidermis, suggest that lipocortin I plays an important role in the regulation of differentiation and proliferation of epidermal keratinocytes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00371600
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  • 4
    Digitale Medien
    Digitale Medien
    Resist system based on the cationic photocrosslinking of poly(4-hydroxystyrene) and polyfunctional electrophiles (1993)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 31 (1993), S. 1-11 
    Details
    ISSN: 0887-624X
    Schlagwort(e): chemical amplification ; photoresist ; poly(p-hydroxystyrene) ; photocrosslinking ; carbocationic ; radiation-sensitive ; crosslinker ; triarylsulfonium salt ; thin-film chemistry ; UV ; E-beam ; x-ray ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: New resist systems based on acid-catalyzed, electrophilic aromatic substitution are described. These new resists show high sensitivity to deep UV and E-beam radiation with values approaching 2 mJ/cm2 and 2 μC/cm2, respectively. The resists are based on a three component system consisting of poly(4-hydroxystyrene), a polyfunctional, low molecular weight, latent electrophile, and a photoactive onium salt used as an acid generator. Irradiation of the resist film produces a latent image of acid dispersed in the matrix. During the postbaking step the photo-generated acid reacts with the latent polyfunctional electrophile and releases a reactive carbocationic intermediate with concomitant liberation of acetic acid. The carbocationic intermediate then reacts with neighboring phenolic moieties in a crosslinking reaction. The substitution reaction liberates a proton, making the process catalytic in nature, thus incorporating the concept of chemical amplification. These highly sensitive materials can be used as nonswelling negative multipurpose resists that function in deep-UV, x-ray or E-beam modes. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pola.1993.080310101
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  • 5
    Digitale Medien
    Digitale Medien
    A new carbon fiber from lignin (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 44 (1992), S. 127-134 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: A new carbon fiber was prepared from the lignin (steam-exploded lignin), which was isolated from steam-exploded birch wood (Betula platyphylla). The lignin was modified to melt thermally on hydrogenolysis. The chloroform soluble and carbon disulfide insoluble fraction (HL) of the reaction products was heated at 300-350°C for 30 min, giving a molten viscous material (HHL). The HHL had a softening point of 110°C and melted at over 145°C to form viscous liquid. When HHL was subjected to a spinning test, according to a conventional fusion spinning method at a speed over 100 m/min, a fine filament could be continuously formed through a pinhole (diameter: 0.3 mm). After the filaments were heated in air at 1-2°C/min up to 210°C, by which time the filament was converted to have an infusible property, the filaments were carbonized by heating from a room temperature to 1000°C at a heating rate of 5°C/min in a stream of nitrogen. The typical properties of the lignin based carbon fiber were as follows: Fiber diameter = 7.6 ± 2.7μ Elongation = 1.63 ± 0.29% Tensile strength = 660 ± 230 MPa; Modulus of elasticity = 40.7 ± 6.3 GPa. The chemical structure of the precursor was remarkably changed from that of the original lignin, indicating the elimination of aliphatic functional groups implied originally in the starting material.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1992.070440113
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  • 6
    Digitale Medien
    Digitale Medien
    A new modification method of exploded lignin for the preparation of a carbon fiber precursor (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 48 (1993), S. 1485-1491 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: A lignin-pitch for carbon fiber was prepared in a high yield from exploded lignin on phenolysis followed by heat treatment under a vacuum. The lignin-pitch had an excellent spinnability in the melt state to form a fine filament. The green fibers were easily made infusible when heated in air at a relatively high heating rate (15-60°C/h). The lignin-based carbon fiber was produced in 43.7% of yield based on a starting material. The tensile strength was more than 400 MPa, indicating that the lignin-based carbon fiber is classified into a general purpose grade. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1993.070480817
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  • 7
    Digitale Medien
    Digitale Medien
    Time-dependent changes in the mechanical properties of zirconia ceramic (1993)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 27 (1993), S. 729-734 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Zirconia has received special attention, mainly because of its high strength and toughness. However, there is some controversy about the time-dependent deterioration of its mechanical properties. To examine the change in mechanical properties of zirconia ceramics in vivo and in vitro, tetragonal zirconia polycrystal pieces were introduced into the medullary cavity of the tibia in Japanese rabbits and animals were sacrificed after 2, 4 and 6 weeks and 6, 12, and 30 mo, respectively. Alumina ceramic and hydroxyapatite (HAP) pieces were used as controls to investigate the differences in biocompatibility. Zirconia showed a bending strength of over 1000 MPa initially, and little time-dependent change in strength was found in both in vivo environments. x-Ray analysis showed little change in the transformation rate, i.e., less than 5 mol % in vivo and in vitro over a period of 3 years. To estimate time-dependent changes in zirconia over a longer period, zirconia pieces were placed in 95°C saline solution for over 3 years and their mechanical properties examined at chosen intervals. No serious decrease of bending strength was found over the 3-year period under these conditions. It is concluded that zirconia can be used clinically because it retains a bending strength of over 700 MPa under any experimental conditions for over 3 years. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820270605
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  • 8
    Digitale Medien
    Digitale Medien
    Barrier-type anodic films on aluminium in aqueous borate solutions: 2 - Film compositions by Rutherford backscattering spectroscopy and nuclear reaction methods (1983)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 5 (1983), S. 252-263 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: Compositions and elemental distributions of anodic films formed on aluminium in aqueous borate electrolytes have been investigated by Rutherford backscattering spectroscopy (RBS) and associated nuclear reaction methods, together with controlled crystallization of film sections in the transmission electron microscope (TEM). Films were found to consist principally of Al2O3; the outer regions of the films were contaminated with boron and, for all cases studied, the inner regions were boron-free. In the absence of film dissolution the contaminated region represented approximately 40% of the film thickness, and this proportion did not vary significantly with changes in current density of film formation. The outer region contained about 1.5 wt% boron, probably as B2O3, and the densities of the inner and outer regions were similar to an accuracy of about 10%.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740050606
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  • 9
    Digitale Medien
    Digitale Medien
    Oxidation of copper and mobility of copper ions during anodizing of an Al - 1.5 wt.% Cu alloy (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 23 (1995), S. 892-898 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The mechanism of oxidation of copper at the alloy/film interface, and the subsequent migration of copper ions in barrier-type films, has been examined for anodizing of an Al - 1.5 wt.% Cu alloy with a prior chemical polishing treatment. Both chemical polishing and anodizing result in formation of a thin layer of alloy at the alloy/film interface, of ∼2 nm thick, that is highly enriched in copper. The layer is present immediately beneath the different types of film formed by chemical polishing and subsequent anodizing, and contains in both cases ∼6 × 1019 Cu atoms m-2. The amount of copper contained within the enriched layer of alloy is not significantly dependent upon the anodizing voltage. During anodic film growth, both aluminium and copper ions are incorporated into the film at the alloy/film interface, on average in their alloy proportions. However, the film is depleted in copper relative to the alloy because copper ions in the film migrate faster than Al3+ ions and, on reaching the film/electrolyte interface, are ejected directly to solution. The mechanism of oxidation of copper is proposed to depend upon the formation, through prior oxidation of aluminium, of copper-rich clusters in the enriched layer of alloy at the alloy/film interface. Individual clusters are oxidized only on achieving a critical size. Consequently, copper is incorporated into the film discontinuously both in time and in position along the alloy/film interface. The films contain a high population density of flaws, which affects the film composition, the uniformity of ionic current, the faradaic efficiency of film growth, and the detailed distributions of copper ions within the films. However, the general features of film growth are compatible with the usual growth mechanism of anodic alumina, with transport numbers of Al3+ and O2-/OH- ions of ∼0.4 and ∼0.6, respectively.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740231307
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  • 10
    Digitale Medien
    Digitale Medien
    Barrier-Type anodic films on aluminium in aqueous borate solutions: 1 - Film density and stopping power of anodic alumina films for alpha particles (1983)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 5 (1983), S. 247-251 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: Stopping powers of an anodic film, formed on aluminium in aqueous ammonium pentaborate solution, have been measured experimentally for α particles, over the energy range 0.7-2.3 MeV. Comparison between the measured stopping powers and theoretically calculated values shows that Bragg's Rule may be applied, with reasonable accuracy (∼5%), to predict the stopping powers of anodic alumina. The density of the barrier-type film was determined by Rutherford backscattering spectroscopy to be 3.10±0.15 × 103 kg m-3.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740050605
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