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  • 1985-1989
  • 1975-1979
  • 1970-1974  (683)
  • 1972  (683)
  • Life and Medical Sciences  (479)
  • Ultrastructure  (115)
  • Biochemistry and Biotechnology  (89)
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Material
Years
  • 1985-1989
  • 1975-1979
  • 1970-1974  (683)
Year
Keywords
  • 101
    Electronic Resource
    Electronic Resource
    The structure of the organ of bellonci of the syncarid crustacean, Anaspides tasmaniae (Thomson) (1972)
    Springer
    Cell & tissue research 132 (1972), S. 431-450 
    Details
    ISSN: 1432-0878
    Keywords: Organ of bellonci (Anaspides tasmaniae) ; Crustacea ; SPX organ ; Receptor ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The organ of Bellonci of Anaspides tasmaniae (Thomson) (Crustacea, Syncarida) is described light and electron microscopically, and a few histochemical tests are reported. Located ventrally in the eyestalk below the medulla interna, the organ is composed of a number of cavities. These are similar in structure in their contents and associated cellular components, which include two types of glia cells delimiting each cavity and the terminal parts of a few dendrites. Each dendrite usually bears two cilia, which project into the cavity where they split up into numerous branches. The organ is supplied by three nerve tracts: two from the medulla terminalis and one from the medulla interna. The sensory pore, which is innervated from the medulla interna, is not closely associated with the organ of Bellonci in Anaspides. No marked secretory activity is detectable by histochemical or ultrastructural observations. It is thought that the organ has a sensory function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306635
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  • 102
    Electronic Resource
    Electronic Resource
    The eyes of Creophilus erythrocephalus F. and Sartallus signatus sharp (Staphylinidae: Coleoptera) (1972)
    Springer
    Cell & tissue research 133 (1972), S. 59-86 
    Details
    ISSN: 1432-0878
    Keywords: Compound eyes ; Coleoptera ; Ultrastructure ; Dark-light-adaptation ; Irregularities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structures of the eyes of two Staphylinid beetles, one large (Creophilus erythrocephalus—18 mm in length) and one small (Sartallus signatus—3 mm in length), were compared. 1. The gross structure of the two eyes is the same: a) biconvex corneal lens, b) crystalline cone of “acone type”, c) narrow crystalline thread and thickening of cone cell extensions just above the rhabdom, d) seven retinula cells plus one basal cell, and e) almost the same corneal refractive index and optical properties of the corneal cuticle. Considerable differences exist in size, shape and arrangement of rhabdom, retinula cells and their axons. The smaller size of the eye of Sartallus is caused by reduction of number of facets rather than cell size, and, in fact, retinula cells and rhabdom have been found to be larger in the smaller beetle. 2. Structural changes during dark-light-adaptation affect crystalline cone, position of screening pigment and size of intercellular spaces between the retinula cells. In the dark-adapted state the cone retracts a little and the crystalline tract becomes wider. A thickening of cone cell extensions occurs just above the rhabdom. Screening pigment migrates to a more distal position and intercellular spaces between the retinula cells considerably increase in size as compared with the light-adapted eye. 3. Both eyes are rich in irregularities. An attempt to classify anomalies of compound eyes has been made. The number of cone cells and principal pigment cells varied, in some cases, from 1–5 and 0–3 respectively. Basal retinula cells did not always contribute to the rhabdom. 4. Interferometrical observations reveal the extreme optical homogeneity of the entire corneal cuticle of the eyes of both species. No layers of different refractive index or optical separation of adjacent ommatidia can be found. The refractive index of the cornea of Creophilus is 1.469; that of Sartallus is 1.488. 5. Functional consequences of the changes which occur during dark-light-adaptation are discussed. Exposure to intense light appears to cause a rapid aging of retinula cells, which is indicated by an increase of onion- and multivesicular bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307068
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  • 103
    Electronic Resource
    Electronic Resource
    Die Ultrastruktur des Gabeschen Organs von Schizophyllum sabulosum L. (Diplopoda, Iuliformia) (1972)
    Springer
    Cell & tissue research 132 (1972), S. 273-285 
    Details
    ISSN: 1432-0878
    Keywords: Diplopoda ; Organ of Gabe ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Das Gabesche Organ von Schizophyllum sabulosum ist paarig und liegt im seitlichen Clypeolabrum. Es wird von Axonen des Nervus labri medialis erreicht, der vorher Seitenzweige abgegeben hat. 2. Die Axone gehören neurosekretorischen Zellen des Protocerebrum an und enthalten Neurosekret. Die Elementargranula sind recht gleichmäßig ellipsoid, der große Durchmesser beträgt ca. 1200 Å. Die Axone enden im Organ und stellen dessen extrinsische Komponente dar. 3. Außerdem gibt es zwei intrinsische Zelltypen: 1) Drüsenparenchymzellen mit axonartigen Fortsätzen und 2) gliaartige Zellen. Die Parenchymzellen bilden Sekret in Form opaker Vakuolen, die deutlich größer als die Neurosekretgranula sind. Auffällig ist das überwiegend vesikuläre endoplasmatische Reticulum. Die Mitochondrien liegen in der Nähe von myelinähnlichen Körpern; ihre Außenmembran ist stellenweise vakuolig vorgewölbt. Die axonartigen Fortsätze enthalten viele längsorientierte Mikrotubuli. 4. Die langen Fortsätze der gliaartigen Zellen umhüllen die Parenchymzellen und die extrinsischen Axone meist in mehreren Schichten. Es gibt aber auch Bereiche, in denen vor allem die Fortsätze der Parenchymzellen und die extrinsischen Axone nackt sind. 5. Das Organ ist gegen das umgebende Hämocoel von einer dicken, lamellierten Stromahülle abgegrenzt. Auch Interzellularräume sind mit Stroma gefüllt. 6. Das Organ wird mit der Cerebraldrüse einiger Chilopoden und gewissen endokrinen Organen anderer Diplopoden und Insekten verglichen.
    Notes: Summary 1. The paired organ of Gabe of Schizophyllum sabulosum is situated in the lateral clypeolabrum. It is innervated by axons of the medial labral nerve, which divides in several branches before reaching the organ. 2. Axons extend from neurosecretory cells of the protocerebrum and contain neurosecretory droplets, which are almost ellipsoid and about 1,200 Å in diameter. The axons terminate in the organ and constitute its extrinsic elements. 3. In addition, there are two types of intrinsic cells: (1) parenchyma cells with axon-like processes and (2) glia-like cells. The parenchyma cells produce secretory material in the form of opaque vacuoles, which are clearly larger than the neurosecretory granules. The preponderantly vesicular endoplasmic reticulum is conspicuous. Also characteristic are the mitochondria, whose superficial membranes are expanded locally, and which lie in the near vicinity of myeline-like bodies. The axon-like processes contain many microtubuli oriented in longitudinal direction. 4. The slender processes of the glia-like cells envelop both parenchyma cells and extrinsic axons usually in several layers; but there are also regions in which the processes of the parenchyma cells and, above all, the extrinsic axons are naked. 5. The organ is delimited from the surrounding hemocoele by a thick laminated stroma. Intercellular spaces are also filled with stroma. 6. The organ is compared with the cerebral gland of some chilopods and with certain endocrine organs of other diplopods and insects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307017
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  • 104
    Electronic Resource
    Electronic Resource
    An ultrastructural study of the body wall muscles of the pentastomid Reighardia sternae dies. 1864 parasitic on the herring gull Larus argentatus pontopp (1972)
    Springer
    Cell & tissue research 130 (1972), S. 12-17 
    Details
    ISSN: 1432-0878
    Keywords: Muscle tissue ; Pentastomid ; Body wall ; Parasite ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the body wall muscle of the pentastomid Reighardia sternae is described. The muscle fibres are separated from one another and form two layers, circular and longitudinal. They are cross-striated with approximately 11 actin filaments surrounding each myosin filament. The T-system consists of simple in-pushings of the sarcolemma. The SR is also simple and forms both dyadic and triadic contacts with the T-system tubules and dyadic contacts with the sarcolemma. Electron-dense inclusions occur, usually in the vicinity of the Z-lines, and it is suggested that these may be composed of unsaturated lipids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306991
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  • 105
    Electronic Resource
    Electronic Resource
    Ultrastructure de l'endomètre humain normal (1972)
    Springer
    Cell & tissue research 133 (1972), S. 47-57 
    Details
    ISSN: 1432-0878
    Keywords: Endometrium ; Human ; Granular stroma cells ; Relaxin ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'ultrastructure générale des cellules K endométriales est semblable chez l'Homme, le Rat et le Singe. Mais les granulations des cellules K humaines sont plus variées que dans ces deux dernières espèces et ont certains aspects suggérant une fonction catabolique. Etant donné cet aspect catabolique et l'existence connue d'une activité phosphatasique acide de ces cellules contenant de la relaxine, les auteurs suggèrent que les cellules K pourraient être des cellules sécrétantes en involution et ques les cellules sécrétant activement la relaxine pourraient avoir un aspect morphologique quelque peu différent de celui des cellules K.
    Notes: Summary The general ultrastructure of endometrial granular stroma cells is similar in man, rat, and monkey. But the granulations of human granular stroma cells are more various than in these two last species and have some aspects suggesting a catabolic function. With respect to this catabolic aspect and the previously shown acid-phosphatase activity of these relaxin-containing cells the authors suggest that the granular stroma cells could be involutive secretory cells and that the active relaxin-secretory cells may have some different morphological aspect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307067
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  • 106
    Electronic Resource
    Electronic Resource
    Zur Morphologie des Ovars von Viviparus contectus (Millet 1813), (Gastropoda, Prosobranchia) (1972)
    Springer
    Cell & tissue research 133 (1972), S. 103-118 
    Details
    ISSN: 1432-0878
    Keywords: Ovary ; Gastropoda ; Follicle cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Ovar von Viviparus contectus besteht, wie licht- und elektronenmikroskopische Untersuchungen ergeben, aus einem langen, terminal verzweigten dünnen Schlauch, dessen Wand von einem Epithel aus Oocyten in allen Entwicklungsstadien und Follikelzellen gebildet wird. Das Vorkommen von Oogonien in den Ovarien adulter Weibchen ist zweifelhaft. Die Follikelzellen sind als Begleitzellen der Oocyten im allgemeinen extrem langgestreckt. Mit ihrer Basis sitzen sie einer dünnen Basalmembran fibrillärer Struktur auf, wie sie bisher nur für Mollusken beschrieben wurde. Die Follikelzellen enthalten große Mengen von Vesikeln, einen bemerkenswert gut ausgebildeten Golgi-Apparat, spärliches rauhes endoplasmatisches Retikulum und zahlreiche heterogene cytosomale Einschlüsse. Charakteristisch für die Zellen sind außerdem in der Längsrichtung liegende cytoplasmatische Mikrotubuli und vereinzelte Kinetosomen. Obwohl keine direkten morphologischen Beziehungen zwischen Follikelzellen und Oocyten festgestellt werden konnten, wird vermutet, daß die Follikelzellen Material degenerierender Oocyten aus dem Lumen des Ovars resorbieren und speichern. Dieses kann den heranwachsenden Oocyten zugute kommen.
    Notes: Summary The ovary of Viviparus contectus was studied light and electron microscopically. It consists of an extended and terminally branched duct, its wall being formed by oocytes of all stages and by follicle cells. The existence of oogonia in the adult females cannot be established with certainty. According to their relationship to the growing oocytes the follicle cells are in general extremely elongated. At the basis they are attached to a thin basement lamina having a fibrillar structure hitherto only known in molluscs. The follicle cells contain many vesicles, a remarkably well-developed golgi apparatus, some rough endoplasmic reticulum, and many heterogeneous cytosomes. Characteristic for these cells are also cytoplasmic microtubules oriented longitudinally along the cell axis. Kinetosomes can rarely be seen. Although there are no apparent direct interrelationships between follicle cells and oocytes, it is suggested that they are engaged in resorbing material of degenerating oocytes from the lumen of the ovary and in storing materials which may be used by the growing oocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307070
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  • 107
    Electronic Resource
    Electronic Resource
    The ultrastructure of neuromuscular systems in Notoplana acticola, a free-living polyclad flatworm (1972)
    Springer
    Cell & tissue research 133 (1972), S. 277-288 
    Details
    ISSN: 1432-0878
    Keywords: Flatworm ; Muscle ; Neuromuscular junction ; Sarcoplasm ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neuromuscular junctions in the marine polyclad flatworm, Notoplana acticola were studied with the electron microscope. Synapses were found between nerve endings and specialized extensions of the muscle cells. Characteristically these processes contained clear cytoplasm with a basal mitochondrion and numerous microtubules aligned parallel to the long axis of the extension. Sarcoplasmic diverticuli which contained the nucleus had granular cytoplasm with an assortment of membranes and organelles. We have proposed the term sarconeural junction to describe synapses between long sarcoplasmic extensions and nerve cells in flatworms as well as other animals. Tight junctions between adjacent contractile portions of muscle cells were common. As groups of cells appeared to be connected by tight junctions or shared common nerve terminals we conjectured that these formed discrete functional motor-units.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307148
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  • 108
    Electronic Resource
    Electronic Resource
    Ultrastruktur und Jodaufnahme der Schilddrüse bei Larven des Krallenfrosches (Xenopus laevis Daud.) (1972)
    Springer
    Cell & tissue research 130 (1972), S. 553-574 
    Details
    ISSN: 1432-0878
    Keywords: Thyroid gland ; Xenopus laevis ; Iodine uptake ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ziel dieser Untersuchung war eine physiologische Deutung der Ultrastruktur von Schilddrüsenepithelzellen bei Larven von Xenopus laevis. Dazu wurden elektronenmikroskopische Bilder mit morphometrischen Methoden quantitativ ausgewertet und die Ergebnisse mit Jodaufnahmedaten verglichen. So konnte die Aktivierung der Schilddrüse durch TSH bei kälteblockierten bzw. hypophysektomierten Tieren mit Veränderungen während der spontanen Metamorphose verglichen werden. Ihrer Ultrastruktur nach sind die Schilddrüsen blockierter Prometamorphoselarven in der Entwicklung auf ein früheres Stadium zurückversetzt. Auch zeigen hypophysektomierte Larven keine spontane Jodaufnahme. Eine einmalige TSH-Injektion läßt dann die Größe und Form der verschiedenen Organellen vorübergehend den Zustand der Kontrollen erreichen. Besonders rasch und stark sprechen einerseits die Kernstruktur, andererseits Mikrovilli und Kolloidtropfenvolumen, welche die Resorption von Thyreoglobulin charakterisieren, auf den TSH-Reiz an. Die Jodaufnahme verläuft langsamer. Auch reagieren die an der Synthese beteiligten Organellen später und schwächer. In der spontanen Metamorphose wachsen die Schilddrüsen, nach Totalvolumen, Cytoplasmavolumen einzelner Zellen und Epithelhöhe beurteilt, bis zum Ende der Metamorphose weiter und nehmen entsprechend mehr Jod auf. Ebenso wird während der ganzen Klimax die Kolloidresorption der einzelnen Zellen stärker. Aus den Ergebnissen wird geschlossen, daß die TSH-Sekretion schon in der Prometamorphose einsetzt und bis gegen Ende der Metamorphose zunimmt.
    Notes: Summary The epithelial cells of the thyroid gland in larvae of Xenopus laevis were investigated with the electron microscope. Morphometrical methods yielded quantitative data on the morphology. The uptake of radioactive iodine was studied in order to obtain information on the physiological activity. Thus changes in the thyroid gland following the injection of TSH in tadpoles which had been hypophysectomized or blocked at low temperature were compared with the development of glands during spontaneous metamorphosis. The thyroid glands of blocked prometamorphic larvae resemble those of younger developmental stages as judged by their ultrastructure. In addition no spontaneous accumulation of iodine is observed. Following an injection of TSH the morphology of the various organelles becomes temporarily identical to that of the controls. These changes are most prominent in the nucleus as well as in the microvilli and the colloid droplets, which characterize the resorption of thyroglobulin. Only a minor effect of the TSH stimulus is found on iodine uptake and on organelles concerned with protein synthesis. During normal metamorphosis an increase is observed in the following parameters: (1) volume of the thyroid gland, (2) volume of the cytoplasm and height of single epithelial cells, (3) resorption of colloid, (4) iodine uptake. This increase persists until the end of the climax. Based on the present results TSH secretion is assumed to start in early prometamorphosis and to rise up to the end of metamorphosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307007
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  • 109
    Electronic Resource
    Electronic Resource
    Zur Morphologie und Funktion der Calcitoninzellen (C-Zellen) der Ratte (1972)
    Springer
    Cell & tissue research 134 (1972), S. 13-30 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Thyroid C-cells ; Ultrastructure ; Tocopherol and Ubiquinone deficiency ; Substitution (d,1-α-Tocopherol, α-Tocopherolquinone)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An der Schilddrüse von Wistar-Ratten wurde der Einfluß der Kost (Laborstandardkost, halbsynthetische-tocopherolhaltige und halbsynthetische-tocopherol-freie Kost) und der subkutanen Injektion von Tocopherol und Tocopherolchinon auf die Ultrastruktur der C-Zellen untersucht. Während nach Standardfütterung die Aktivität der C-Zellen der Norm entspricht, werden Syntheseleistung und Sekretabgabe dieser Zellelemente durch die halbsynthetische-tocopherolhaltige Kost gesteigert und im alimentären Tocopherol-Ubichinonmangel wieder gedämpft. Der elektronenmikroskopisch erkennbare Gehalt der C-Zellen an Sekretgranula entspricht der in vitro ermittelten Calcitoninaktivität. Die Beziehungen zwischen den Funktionskreisen von Thyreozyten und Calcitoninzellen werden diskutiert.
    Notes: Summary The influence of different diets (standard laboratory diet, semisynthetic tocopherol-containing and semisynthetic tocopherol-free diet) and the influence of subcutaneous injections of tocopherol and tocopherolquinone upon the ultrastructure of the C-cells in the thyroid gland of Wistar rats were investigated. While the activity of the C-cells is quite normal after feeding a standard diet, the synthetic and secretory activities of these cells show an increase with a semisynthetic diet containing tocopherol, but a decrease during alimentary lack of tocopherol and tocopherolquinone. The amount of secretory granules in the C-cells as seen with the electron microscope corresponds with the in vitro-activity of extracted calcitonin. The relations between the functional system of the thyrocytes and that of the C-cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307233
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  • 110
    Electronic Resource
    Electronic Resource
    The ultrastructure of the A1 sensilla on the posterior surface of the clypeo-labrum of Locusta migratoria migratorioides (R and F) (1972)
    Springer
    Cell & tissue research 134 (1972), S. 539-554 
    Details
    ISSN: 1432-0878
    Keywords: Chemoreceptors ; Locusta ; Clypeo-labrum ; Light and electron microscopy ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The A1 sensilla are asymmetric in distribution. Each sensillum contains five bipolar neurons, the distal regions of which extend to the tip of a cuticular cone which has a terminal pore. This was shown with the scanning electron microscope, but was not seen in section. The receptor-lymph cavity of the tormogen cell and the basal cavity of the neurilemma cell contain an electron-dense material unlike other contact chemoreceptors of Locusta prepared for electron microscopy in the same manner. Isolated axons of unknown function occur below the basement membrane and also between epidermal cells surrounding the sensillum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307673
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  • 111
    Electronic Resource
    Electronic Resource
    Ultrastructure of the anuran pars intermedia following severance of hypothalamic connection (1972)
    Springer
    Cell & tissue research 131 (1972), S. 545-557 
    Details
    ISSN: 1432-0878
    Keywords: Pars intermedia ; Hypothalamus ; Anurans ; Hormone release ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the pars intermedia of Rana catesbeiana tadpoles was studied following isolation from the hypothalamus, in vivo after sectioning of the pituitary stalk, and in vitro after implantation of the pituitary into a piece of tail fin. Both experimental procedures were followed by rapid and sustained skin darkening. Pituitaries from normal light and dark adapted tadpoles served as controls. In 4-hour disinhibited glands, melanotrophs revealed hyperactive Golgi bodies, colloid vesicles (1–2 microns) in close proximity to axon terminals, and no apparent loss of secretory granules. At 24 hours extracellular colloid adjacent to axon terminals was found, and extensive arrays of RER appeared in the melanotrophs. Obvious granule loss from secretory cells occurred within a week, by which time the cytoplasm was occupied by large cisterns of SER and RER and abundant free ribosomes. Dense core vesicles (600–900 Å) in aminergic nerve terminals disappeared shortly after isolation of the pituitary from the hypothalamus, and only decreasing numbers of translucent vesicles (200–300 Å) were found. The functional significance of these changes is discussed, with particular emphasis on the mode of acute hormone release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306970
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  • 112
    Electronic Resource
    Electronic Resource
    Oligodendrocytes in the normal and chronically de-afferented lateral geniculate body of the monkey (1972)
    Springer
    Cell & tissue research 135 (1972), S. 115-127 
    Details
    ISSN: 1432-0878
    Keywords: Oligodendroglia ; Monkey ; De-afferentation ; Ultrastructure ; Densebodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopical examination of the norma and de-afferented ‘laterall geniculate body’ of the monkey following paraformaldehyde-glutaraldehyde vascular perfusion revealed distinctive morphological features of different types of oligodendrocyte. These cells were normally situated as perineuronal satellites or in relation to axons and capillaries. A wide range of nuclear and cytoplasmic densities were displayed by both satellite and interfascicular oligodendrocytes. The following distinctive features for the identification of ligodendrocytes were utilised: the presence of large quantities of free ribosomes and ribosomal rosettes, microtubular profiles, dense marginal aggregation of nuclear chromatin together with light patches and numerous nuclear pores; but the absence of broad cytoplasmic processes, glycogen and gliofibrils. Circumferential perinuclear organization of the cytoplasmic organelles was typical of oligodendrocytes. Particular attention was paid to perineuronal satellite cells in view of the known transneuronal atrophy in the de-afferented geniculate body. Some cells having a nuclear pattern of oligodendrocytes but showing hyalinisation of perikaryon were seen in de-afferented laminae. A notable feature was the presence of variegated “osmiophilic bodies” in the perikaryon of oligodendrocytes also situated in the de-afferented laminae. A cell type combining the features of oligodendrocytes and astrocytes was classified as ‘intermediate neuroglia’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307092
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  • 113
    Electronic Resource
    Electronic Resource
    Les cellules corticotropes de l'hypophyse d'un Amphibien après interrénalectomie (1972)
    Springer
    Cell & tissue research 132 (1972), S. 333-364 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Amphibians ; Corticotrophs ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'identification des cellules responsables de l'élaboration de la corticotropine (ACTH) a été envisagée chezRana esculenta. Les effects causés par l'interrénalectomie au niveau de l'hypophyse ont été étudiés parallèlement par immunofluorescence et au microscope électronique. Au microscope à fluorescence, les cellules détectées chez les animaux témoins avec un antisérum anti-synachten1 bordent en grand nombre les capillaires des zones médiorostrale et ventrale du lobe antérieur. Deux jours après interrénalectomie, le nombre de cellules fluorescentes décroît, douze jours après l'intervention, il ne subsiste pas d'éléments fluorescents dans cette zone. Au microscope électronique, les cellules corticotropes présentent de fines granulations d'environ 200 mμ de diamètre. Après interrénalectomie bilatérale, ces cellules sont fortement stimulées, elles sont sujettes à d'importantes modifications morphologiques; l'aspect morphologique des autres catégories de cellules antéhypophysaires, par contre, n'est pratiquement pas modifié. Douze jours après l'opération, la plupart de ces cellules sont dégranulées, l'ergastoplasme et l'appareil de Golgi sont bien développés. Ces observations suggèrent que les cellules péricapillaires de la moitié rostrale de lapars distalis sécrètent l'hormone corticotrope.
    Notes: Summary Identification of the cell types responsible for the production of adrenocorticotropin (ACTH) was performed inRana esculenta. The effects of interrenalectomy on the pituitary cells were studied as well by immunofluorescence, as by electron microscopy. In control animals, the ACTH cells studied by immunofluorescence are numerous around the blood vessels of the medio-rostral and medio-ventral part of the anterior lobe. Two days after interrenalectomy the number of fluorescent cells decreases. Twelve days after, the operation all the fluorescent cells disappeared. The fine structure of the corticotrophs is characterized by the presence of small secretory granules (200 mμ). After bilateral interrenalectomy this cell type is markedly stimulated; it displays striking morphological changes, while the morphology of the other pituitary cell types is not considerably modified. Twelve days after operation most of these cells are degranulated, the rough endoplasmic reticulum and the Golgi apparatus are well developed. These findings suggest that the pericapillary cells of the rostral half of thepars distalis produce the adrenocorticotrophic hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02450713
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  • 114
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    Ultrastruktur des Pseudoculus vonEosentomon (Protura, Insecta) (1972)
    Springer
    Cell & tissue research 135 (1972), S. 539-551 
    Details
    ISSN: 1432-0878
    Keywords: Pseudoculus ; Eosentomon (Protura, Insecta) ; Sense organ ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In der Feinstruktur unterscheidet sich der Pseudoculus vonEosentomon nicht wesentlich von dem der Acerentomiden. Durch einen Endokutikulaporus treten die dendritischen Fortsätze zweier Sinneszellen, jeweils umgeben von einer Hüllzelle, in den Außenraum des Pseudoculus ein. Der Außenraum wird nach distal von einer äußeren Kutikulaschicht — vermutlich Epikutikula — abgeschlossen. Sie vermittelt durch regelmäßig angeordnete lange Spalten die Verbindung zur Außenwelt. Am Grunde der Spalten finden sich Porentubuli, die mit den Hüllzellen oder den distalen Fortsätzen der Dendriten Kontakt haben können. Aus der Feinstruktur kann geschlossen werden, daß der Pseudoculus als Chemo-, Hygro- und/oder Thermorezeptor fungiert.
    Notes: Summary Concerning its fine structure the pseudoculus of Eosentomon is quite similar to that of Acerentomide Protura. There are two sensory cells innervating the organ. From each of them one dendritic process derives, surrounded by one enveloping cell. The processes of these four cells enter the distal cavity of the pseudoculus through a pore in the endocuticular layer. The cuticular layer of the cap seems to consist of epicuticle only. It is furrowed by long clefts connecting the distal cavity of the organ with the outside. Poretubules insert at the base of the clefts and may have contact with the cell membranes of both enveloping cells and dendritic processes. According to its structure the pseudoculus may function as chemo-, hygro- and/or thermoreceptor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583435
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    Nervous system of the snailHelix aspersa (1972)
    Springer
    Cell & tissue research 135 (1972), S. 473-482 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretory nerves ; Glial and pigment cells ; Neural sheath ; Gastropods ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of neurosecretory nerves and endings associated with the sheath of the infraesophageal ganglion ofHelix aspersa is described. The sheath is a neurohemal organ whose vascularized stroma receives both monoaminergic and peptidergic endings. The latter occur along the surface of the nerves or scattered within the stroma. They include a complex population of vesicular profiles. The granular vesicles (1300–3000 Å in diameter) exhibit structural modifications that may be related to the intra-axonal release of their neurohormones. The agranular vesicles (500–2000 Å in diameter) occur in large numbers and lie mostly adjacent to the axon surface. Synaptoid specializations seem to represent active sites for the extracellular discharge of neurosecretory material. The monoaminergic endings so far studied lack synaptoid specializations and contain small granular (800–1300 Å in diameter) and agranular (700 Å in diameter) vesicles. Two kinds of non-neural cells are associated with the nerves: glial cells and melanocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583431
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051360101
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    Ultrastructural changes in the pars distalis of the maturing male rat (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 53-77 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Anterior pituitary glands of male rats (2, 3, 5, 8, 12, 25, 36, 52, 56, and 62 days of age) were processed for electron microscopy. During early postnatal stages secretory cells are found in various stages of differentiation and comparatively few secretory granules are seen. Nuclei are mostly irregular, and the nucleo-cytoplasmic ratio is large. Many free ribosomes are present; the endoplasmic reticulum is generally sparse and the Golgi complex small or invisible. Cells are of variable shape, and numerous cytoplasmic processes project into large intercellular spaces. Many electron-dense cells which often contain myelinlike figures are seen. Lysosomes and lysosomal precursors are frequently found in secretory cells, predominantly in somatotrophs, of all immature glands. Mitotic figures are numerous in early stages after brith and decrease in number as the gland grows in size. A gradual increase in cytoplasmic volume with concomitant differentiation of cytoplasmic components as well as accumulation of secretory granules, accompanied by loss of myelin-like figures and decrease in the number of electron-dense cells, is observed as the animal reaches the prepuberal stage. Few lysosomes are seen in cells of mature glands. At 36 days of age all secretory cells seem to have differentiated, and morphological features as well as granule content show little change until puberty is reached. Gonadotrophs attain their characteristic morphology later than other cells. Cilia are observed in all developmental stages but are relatively infrequent in the mature gland. The described ultrastructural characteristics reflect the degree of maturation as well as the functional capacities of secretory cells at particular stages of development.
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    URL: http://dx.doi.org/10.1002/jmor.1051360104
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    The fine structure of an absorptive epithelium in a viviparous teleostWork performed under the auspices of the U.S. Atomic Energy Commission, and with support from the National Science Foundation, grant GB2378. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 109-129 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Embryos of the viviparous teleost, “Characodon” eiseni, have unusual anal processes that function only during gestation and are lost shortly after birth. This study was undertaken to determine if the fine structure of the process epithelium supports the assumption that these cells have an absorptive function. The process epithelium is a single layer of columnar cells. At peak activity intercellular spaces become very large and isolate individual cells which simultaneously lose much of their cell mass. The cells are characterized by microvilli on their free surface, much pinocytic activity and by the formation of at least four different kinds of vesicles. There is much evidence that these vesicles fuse together. A distinctive characteristic of these cells is a system of tubules and flattened cisternae that somewhat resemble the endoplasmic reticulum yet they differ from it in several respects. It is suggested these profiles aid in fragmenting the cell at periods of peak absorptive activity, thereby increasing the cell surface. Possible mechanisms of food absorption are considered. The fine structure of these cells supports the contention that these embryonic processes serve as absorptive organs during gestation.
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    URL: http://dx.doi.org/10.1002/jmor.1051360106
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    The spermatozoon of Branchiostoma lanceolatum L (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 211-226 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Under the electron microscope, the spermatozoon of Branchiostoma lanceolatum shows a spherical nucleus deeply grooved along its caudal third, a bistratified acrosome enriched by plentiful subacrosomal material, two centrioles, mitochondria fused into a single mass surrounding the centriolar region which is highly asymmetrical, a 9 + 2 flagellum tilted with respect to the longitudinal symmetry axis of the nucleus. The sperm of Branchiostoma shares the overall features of that of the Tunicata and fits in perfectly with the phylogenetic position of the Leptocardia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051360206
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    The early development of substrate-brooding cichlids (Teleostei: Cichlidae) with a discussion of a new system of staging (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 255-272 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The early development of five species of substrate-brooding cichlid (Aequidens pulcher; Cichlasoma bimaculatum; C. biocellatum; C. nigrofasciatum and Hemichromis bimaculatus) is described. Heterochrony in these species renders the standard system of staging inadequate for comparative study. A new system is proposed in which the period of development is resolved into five overlapping phases, each phase corresponding to a dissociable ontogenetic process. This form of staging permits comparison in a way which neither obscures the basic uniformity of cichlid development nor masks the differences between the species.Two special features are discussed: the universal presence in substrate-brooding cichlids of three pairs of embryonic adhesive organs; and the apparent presence of non-neural crest pigment on the embryos and alevins.
    Additional Material: 15 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051360302
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    Ultrastructural studies of differentiation in the oocyte of the polyclad turbellarian, Prostheceraeus floridanus (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 273-295 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Oocyte differentiation in the polyclad turbellarian Prostheceraeus floridanus has been examined to determine the nature of oogenesis in a primitive spiralian. The process has been divided into five stages. (1) The early oocyte: This stage is characterized by a large germinal vesicle surrounded by dense granular material associated with the nuclear pores and with mitochondria. (2) The vesicle stage: The endoplasmic reticulum is organized into sheets which often contain dense particles. Vesicles are found in clusters in the cytoplasm, some of which are revealed to be lysosomes by treatment with the Gomori acid phosphatase medium. (3) Cortical granule formation: Cortical granules are formed by the fusion of filled Golgi vasuoles which have been released from the Golgi saccules. The association between the endoplasmic reticulum and Golgi suggests that protein is synthesized in the ER and transferred to the Golgi where polysaccharides are added to form nascent cortical granules. (4) Yolk synthesis: After a large number of cortical granules are synthesized, yolk bodies appear. They originate as small membrane-bound vesicles containing flocculent material which subsequently increase in size and become more compact. Connections between the forming yolk bodies and the endoplasmic reticulum indicate that yolk synthesis occurs in the ER. (5) Mature egg: In the final stage, the cortical granules move to the periphery and yolk platelets and glycogen fill the egg. At no time is there any evidence of uptake of macromolecules at the oocyte surface. Except for occasional desmosomes between early oocytes, no membrane specialization or cell associations are seen throughout oogenesis. Each oocyte develops as an independent entity, a conclusion supported by the lack of an organized ovary.
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    URL: http://dx.doi.org/10.1002/jmor.1051360303
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    The tracheae of water mites (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 327-335 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The water mites of standing waters have evolved a novel respiratory system consisting of numerous independent tracheae of tracheolar dimensions. Each trachea has a portion of its length lying directly under the cuticle and one or both ends of the trachea turn into the body to supply some organ. There is no fusion of tracheae to form trunks. Areas of dense tracheation dorsal to the legs supply the leg muscles, and sometimes there is a distinct area of the venter that supplies the muscles of the mouthparts.
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/jmor.1051360306
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    Effects of ovariectomy and steroid replacement on the genital tract of the viviparous lizard, Xantusia vigilisPart of this work was done at the Department of Physiology-Anatomy, University of California, Berkeley, supported by NIH grant AM-11161 and by Population Council grant M70.69.C to Dr. Charles S. Nicoll. This work is partly supported by NRC grant A1597 to Dr. N. E. Henderson. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 313-325 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Two glandular components are described in the genital tract of Xantusia: tubal glands in the Fallopian tube and goblet cells in the uterine villi. Sperm or seminal receptacles occur between adjacent villi in the uterus.Forty ovariectomized lizards carrying a silk loop in the wall of the left uterus were treated for two weeks with either progesterone, estradiol-17 β, progesterone plus estradiol or vehicle. Uteri with loops serving as a local irritant, did not differ significantly from the contra-lateral uteri in any group, hence a response similar to the deciduomal reaction of mammals is not found in this lizard.The weight of the genital tract is similar in sham-operated and in ovariectomized lizards injected with either progesterone or the vehicle. Maximal increase in weight of the tract is noted with estradiol treatment, while simultaneous administration of both steroids is followed by a moderate increase of oviducal weight. Tubal glands and sperm receptacles in ovariectomized lizards injected with either the vehicle or progesterone are smaller than those of the sham-operated or ovariectomized lizards treated with estradiol or with estradiol plus progesterone. Goblet cells are small and lack secretory granules in ovariectomized lizards injected with either the vehicle, or with estrogen or progesterone alone. Both steroids, given together, restore the size and apparent secretory activity of the goblet cells. It is concluded that in this viviparous species, both estrogen(s) and progestin(s) are essential for the maturation of the genital tract in the preovulatory stage.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051360305
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    Evolutionary morphology of the caecilian urogenital system. IV. The cloaca (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 353-365 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The gross and microscopic anatomy of male and female cloacae of caecilians (Amphibia: Apoda or Gymnophiona) is described and analyzed in terms of structure and function. The arrangement of musculature and cloacal accessory structures is species-specific in males. Contraction of certain cloacal and body wall musculature facilitates eversion of the male cloaca for use as an intromittent organ. The cloacae of females show less marked morphological differences from species to species, and are modified as receptors of male phallodea.
    Additional Material: 17 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051360308
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051360401
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    Studies on the neurosecretory system and retrocerebral endocrine glands of Nezara viridula Linn. (Heteroptera: Pentatomidae) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 337-351 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The neurosecretory system and retrocerebral endocrine glands of Nezara viridula Linn. have been described on the basis of in situ preparations and histological sections employing the paraldehyde fuchsin (PF) and performic acid-victoria blue (PAVB) techniques.In the brain of N. viridula, there are two medial groups-each consisting of five neurosecretory cells which belong to A-type. The lateral neurosecretory cells are absent. The axons of the two groups of medial neurosecretory cells (MNC) compose the two bundles of neurosecretory pathways (NSP) that decussate in the anterodorsal part of the protocerebrum. The two pathways, after the cross-over, run deep into the protocerebrum and deutocerebrum and emerge as NCC-I from the tritocerebrum. The nervi corporis cardiaci-I (NCC-I) of each side which are heavily loaded with NSM terminate in the aorta wall. Thus, the neurosecretory material (NSM), elaborated in the medial neurosecretory cells of the brain, is stored in the aortic wall and nervi corporis cardiaci-I (NCC-I). The NCC-II are very short nerves that originate from the tritocerebrum and terminate in the corpora cardiaca (CC) of their side. Below the aorta, but dorsal to the oesophagus, lie two oval or spherical corpora cardiaca. A corpus allatum (CA) lies posterior to the corpora cardiaca (CC). The corpora cardiaca do not contain NSM; only the intrinsic secretion of their cells has been occasionally observed which stains orange or green with PF staining method. The corpus allatum sometimes exhibits PF positive granules of cerebral origin. A new connection between the corpus allatum and aorta has been recorded. The suboesophageal ganglion contains two neurosecretory cells of A-type which, in structure and staining behaviour, are similar to the medial neurosecretory cells of the brain. The course and termination of axons of suboesophageal ganglion neurosecretory cells, and the storage organ for the secretion of these cells have been reported. It is suggested that the aortic wall and NCC-I axons function as neurohaemal organ for cerebral and suboesophageal secretions.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051360307
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    A light and electron microscopic study of sensory organs and associated structures in the foreleg tibia of the cricket, Gryllus assimilisPortion of a dissertation submitted to the Graduate Medical Sciences Council of the University of Tennessee in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Anatomy.This investigation was supported by PHS Training grant GM-00202-10 from the National Institutes of General Medical Sciences. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 263-327 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Early descriptions of insect sensory organs included three presumed mechanotransducers in the tibia of Orthoptera, namely the subgenual organ, the intermediate organ, and the tympanal organ. This investigation re-evaluates the light microscopic appearances of these organs in the foreleg tibia of the cricket, Gryllus assimilis, initially described by Herbig in 1902.The study also examines the fine structure of the subgenual and intermediate organs and provides the first fine structure analysis of these structures.The subgenual and intermediate organs are typical scolopophorus organs suspended perpendicular to each other within the dorsal hemolymph canal. Each is innervated by dendrites from the anterior ganglion. Neither the subgenual, intermediate, nor tympanal organ is structurally related to the larger posterior tympanic membrane.The study shows that the tibial tympanal organ in the cricket is not a scolopophorus organ, but consists of highly modified epithelium, associated with the anterior tympanic membrane, and receiving innervation from the anterior ganglion.
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    Development of electric organs of Gymnarchus niloticus (Fam. Gymnarchidae): I. Origin and histogenesis of electroplates (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 375-385 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The development of the electric organs of Gymnarchus niloticus has been studied and the origin and histogenesis of an electroplate worked out. A segmental origin of the electroplate is reported for the first time for this fish. Light has been thrown on many hitherto obscure phenomena, viz., growth of core girth, loss of transverse striations on the myofibrillar elements, differentiation of electroplate polarities, shortening in length of the electroplate etc. The transverse striations of the myofibrillar bundle of the electroplate primordium progressively disappear with development owing to splitting apart of the constituent myofilaments and consequent loss of their parallel order, and not to degeneration of the myofibrillar bundle. The excessive growth of the core girth of the electroplates is caused by the deposition of some kind of interfibrillar substance probably secreted by the peripheral cytoplasm.
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    Fine structure and differentiation of ascidian muscle. I. Differentiated caudal musculature of Distaplia occidentalis tadpolesThis investigation was supported by NIH Developmental Biology Training grant 5-T01-HD00266 to the University of Washington and NSF Research grant GB 5394. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 349-373 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structure of the caudal muscle in the tadpole larva of the compound ascidian Distaplia occidentalis has been investigated with light and electron microscopy. The two muscle bands are composed of about 1500 flattened cells arranged in longitudinal rows between the epidermis and the notochord. The muscle cells are mononucleate and contain numerous mitochondria, a small Golgi apparatus, lysosomes, proteid-yolk inclusions, and large amounts of glycogen. The myofibrils and sarcoplasmic reticulum are confined to the peripheral sarcoplasm.Myofibrils are discrete along most of their length but branch near the tapered ends of the muscle cell, producing a Felderstruktur. The myofibrils originate and terminate at specialized intercellular junctional complexes. These myomuscular junctions are normal to the primary axes of the myofibrils and resemble the intercalated disks of vertebrate cardiac muscle. The myofibrils insert at the myomuscular junction near the level of a Z-line. Thin filaments (presumably actin) extend from the terminal Z-line and make contact with the sarcolemma. These thin filaments frequently appear to be continuous with filaments in the extracellular junctional space, but other evidence suggests that the extracellular filaments are not myofilaments.A T-system is absent, but numerous peripheral couplings between the sarcolemma and cisternae of the sarcoplasmic reticulum (SR) are present on all cell surfaces. Cisternae coupled to the sarcolemma are continuous with transverse components of SR which encircle the myofibrils at each I-band and H-band. The transverse component over the I-band consists of anastomosing tubules applied as a single layer to the surface of the myofibril. The transverse component over the H-band is also composed of anastomosing tubules, but the myofibrils are invested by a double or triple layer. Two or three tubules of sarcoplasmic reticulum interconnect consecutive transverse components.Each muscle band is surrounded by a thin external lamina. The external lamina does not parallel the irregular cell contours nor does it penetrate the extracellular space between cells. In contracted muscle, the sarcolemmata at the epidermal and notochordal boundaries indent to the level of each Z-line, and peripheral couplings are located at the base of the indentations. The external lamina and basal lamina of the epidermis are displaced toward the indentations.The location, function, and neuromuscular junctions of larval ascidian caudal muscle are similar to vertebrate somatic striated muscle. Other attributes, including the mononucleate condition, transverse myomuscular junctions, prolific gap junctions, active Golgi apparatus, and incomplete nervous innervation are characteristic of vertebrate cardiac muscle cells.
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051380401
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    Aspects of the ultrastructure of the alimentary canal and respiratory ducts in Xenopus laevis larvae (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 387-405 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The pharynx, intestine and respiratory duct of Xenopus laevis were examined by light and electron microscopy, at different stages of the metamorphic cycle, through climax.It is well known that preclimactic larvae are suspension feeders and that after climax specimens feed on solid food. It is shown that the histology of the pharynx and the alimentary canal changes in adaptation to the change in the mode of feeding.Suspension feeding utilises ciliary activity but after climax cilia have disappeared from the pharynx and alimentary canal, when new neuromuscular mechanisms are utilised in feeding.Other morpho-histological changes in various intestinal and respiratory tissues are likewise considered in terms of functional activity.
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    The maxillae of Drosophila melanogaster as revealed by scanning electron microscopy (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 451-456 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The scanning electron micrographs show the external morphology of the maxillae of Drosophila melanogaster. Specifically, they illustrate the patterning of the different types of chemo-receptive sensilla on the maxillary palpi making possible a clearer understanding of the structure of the tiny maxillary lobes. It appears that the maxillary lobes act as “cleaning brushes” during the feeding process.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051380405
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    The fine structure of a cephalic sensory receptor in the copepod Doropygus seclusus Illg (Crustacea: Copepoda: Notodelphyidae) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 407-431 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A cephalic organ of presumed sensory function is described in nauplii and copepodids of the ascidicolous copepod Doropygus seclusus Illg. The receptor, located bilaterally in the anterodorsal head region, is composed of dendrites of extra optic protocerebral origin which have ciliary protrusions with basal bodies, no rootlets, and a basal infrastructure of the 9 + 0 type. The cilia do not branch and their distal terminations contain only one to four microtubules. In nauplii and free-living copepodids, a large epidermal supporting cell encapsulates the end of one dendrite and its cilia in a sac. Other dendrites and their cilia pass through the supporting cell and, terminally, the cilia escape to form a whorled fascicle which contacts the anterolateral cephalic cuticle. The latter end organ reaches its greatest development in the second copepodid stage  -  the stage which infects the ascidian. All of the symbiotic stages of the copepod have only a proportionately smaller end organ of the saccular type and apparently lack the end organ consisting of whorls of ciliary ends. The function of the receptor is unknown, but it is suggested that the end organ which disappears in the symbiotic stages functions in second copepodids in host recognition.
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    URL: http://dx.doi.org/10.1002/jmor.1051380403
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    The ultrastructure of developing wings in the giant silkmoth, Hyalophora cecropia. I. Generalized epidermal cells (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 1-21 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of wing epidermis of the giant silkmoth, Hyalophora cecropia, was studied during pupal diapause and the first half of development to the adult. In diapause, the generalized epidermal cells are characterized by many free ribosomes, some vesicles and small lamellae of rough endoplasmic reticulum, some scattered short mitochondria and a few small Golgi complexes. During the early states of post-diapause development, before and after the time of apolysis (separation of the epidermis from the overlying cuticle), there is a marked increase in structures often associated with synthetic functions, such as polyribosomes, lamellate rough endoplasmic reticulum and Golgi complexes. On day five of post-apolysis development, just after the appearance of scale-forming and socket-forming cells, the generalized epidermal cells lay down the cuticulin layer of the adult cuticle. At this stage and later, the polyribosomes and lamellate rough endoplasmic reticulum decrease in abundance. Cell nuclei show three phases of temporary transition from predominantly lobed to predominantly round profile, which correspond to periods of reported DNA synthesis. Throughout this developmental process, therefore, there is good correlation of fine structure with changes in macromolecular synthesis recorded elsewhere.
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  • 135
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    Aspects of the circulatory system of Pollicipes polymerus J. B. Sowerby (Cirripedia: Thoracica) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 79-107 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The circulatory system of Pollicipes polymerus exhibits a high degree of organization which precludes it from being referred to as an open system. The system is arbitrarily divided into four parts: (1) the circulation of the peduncle and mantle; (2) the distributive circulation of the body, which provides hemolymph to most of the cephalic gut, to the maxillary gland, and to the cirri; (3) the peripheral circulation which distributes blood from the cirri to the peripheral areas of the thoracic region, to most of the thoracic gut, and from the scutal sinus to the peripheral areas of the cephalic region; and (4) the collecting circulation, which conveys hemolymph mostly from the peripheral circulation of the body to the peduncle. There also may be a circulation that is comparable to the vertebrate lymphatic system.Pumping of hemolymph can be attributed to three pairs of skeletal muscles that compress the dorsolateral channels. These muscles are unique for crustacean muscles in that they do not appear to be striated.The rostral vessel appears to be a vestige of a heart in which the pump muscles have been lost. There is a similarity of the rostral vessel to the heart of Calanus finmarchicus (a copepod). This is additional evidence linking the cirripeds with the copepods within the Maxillopoda.Electron microscope observations of the walls of the midsagittal vessels indicate that there is a more or less random layering of cellular and noncellular elements within the wall. Muscle cells appear to be incorporated in the vessel wall.
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  • 136
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    Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animalsResearch sponsored by the U. S. Atomic Energy Commission under contract with Union Carbide Corporation. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 153-179 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Oogenesis in the anuran Xenopus laevis can be divided into six stages based on the anatomy of the developing oocyte. Stage I consists of small (50 to 100 μ) colorless oocytes whose cytoplasm is transparent. Their large nuclei and mitochondrial masses are clearly visible in the intact oocyte. Stage II oocytes range up to 450 μ in diameter, and appear white and opaque. Stage I and II are both previtellogenic. Pigment synthesis and yolk accumulation (vitellogenesis) begins during Stage III. Vitellogenesis continues through Stage IV (600 to 1000 μ), the oocytes grow rapidly, and the animal and vegetal hemispheres become differentiated. By Stage V (1000 to 1200 μ) the oocytes have nearly reached their maximum size and yolk accumulation gradually ceases. Stage VI oocytes are characterized by the appearance of an essentially unpigmented equatorial band. They range in size from 1200 to 1300 μ, are postivtellogenic and ready for ovulation. These stages of oocyte development have been correlated with physiological and biochemical data related to oogenesis in Xenopus.
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  • 137
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    Ultrastructural contributions to the identification of cell types in the lizard epidermal generation (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 191-209 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of the epidermis at different stages of the shedding cycle has been studied in Anolis carolinensis. Cells of the germinal layer are morphologically similar at all stages in the cycle. Immediately after leaving the germinal layer all daughter cells resemble one another closely. However, they later acquire specific ultrastructural features that enable them to be classified into six distinct fully differentiated types corresponding to the grouping previously set forth by light microscopy. A comparison of cytoplasmic filament size with the known X-ray diffraction data suggests that the Oberhautchen and β-layer contain a protein similar to that of avian feather; the protein in the α-layer and lacunar tissue is similar to that in mammalian hair, and the mesos layer cells probably contain a mixture of feather and hair-like proteins. The nature of the amorphous cytoplasmic material in the mature clear layer is as yet unknown.
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  • 138
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    Comparative observations on the morphology of the mammalian periovarial sac (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 247-254 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The interspecific variation in the positional and mesenteric relationship of the mammalian oviduct to the ovary observed among 41 species is summarized by distinguishing eight morphological types of relationship. The recognition of types is based on wide species differences in: the position of the oviduct with reference to the mesosalpinxal fold and ovary; the extent to which oviducal mesenteries enclose the ovary in a periovarial sac; the degree of closure and method of formation of the peritoneal opening to the periovarial sac; and the morphology of the oviduct.
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    URL: http://dx.doi.org/10.1002/jmor.1051360208
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  • 139
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051360301
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  • 140
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    Observations on the integument of the water mite Arrenurus major (Acari: Parasitengona)A section of the thesis submitted by the author in partial fulfillment of requirements for the degree of Doctor of Philosophy at Cornell University. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 433-449 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study of the integument of the aquatic mite Arrenurus major Marshall is presented. When the cuticle is examined with the unaided eye and the light microscope, it appears to possess numerous tiny pits. However, scanning electron micrographs of the cuticle reveal that it is a solid surface with topographical sculpturing of the epicuticle, indicating that the “pits” are an internal phenomenon. In cuticle which has been sectioned, areas devoid of cuticular material beneath the thin exocuticle are revealed. These areas are the pits which are goblet-shaped.The integument consists of five major strata. These are from the outside to the inside: (1) a superficial layer with a maximum observed thickness of 725 Å, (2) an epicuticle with a thickness of about 900 Å and composed of at least four sublayers, (3) an exocuticle with a thickness of about 1.5 Å. Fibers of the exocuticle are arranged in a Bouligand pattern and exhibit a regularly occurring discontinuity with a spacing of 200 Å. (4) An endocuticle ranging from 15 to 20 μ in thickness. The endocuticle is characterized by bandings which superficially resemble the lamellae of insects but are not homologous, microfibers which exhibit a preferred orientation, and the presence of the pits; and (5) an epidermis lying beneath the endocuticle and extending into the pits.Pore canals are present only in the exocuticle and have their origin at the apices of the pits. The pore canals contain a central filament, and a plug is present just beneath the epicuticle.
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    URL: http://dx.doi.org/10.1002/jmor.1051380404
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  • 141
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    Lymphoid tissue in the snapping turtle, Chelydra serpentinaSupported by United States Public Health Service grant AI 10679-01 to M.B. and National Science Foundation grant GB 17767 and GRSG-USPHS 1-FOL-FR-05354, to E.L.C. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 487-497 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This report presents light microscopic descriptions of lymphoid organs and aggregates in the snapping turtle Chelydra serpentina. The thymus and spleen were found to be similar to those of other ectothermic vertebrates. Certain gut associated lymphoid aggregates suggest the presence of reptilian equivalents of tonsils, Peyer's patches and the avian bursa of Fabricius. Lymphoid aggregates located in the axillary and inguinal regions were apparent for the first time in an ectothermic vertebrate. These are of particular interest since they may represent ancestors of true lymph nodes in analogous locations in mammals. It is concluded that the snapping turtle is not deficient in lymphoid tissue, although there is a conspicuous absence of typical germinal centers, characteristic of mammalian organs.
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    URL: http://dx.doi.org/10.1002/jmor.1051380408
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  • 142
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    Suppression of the allograft response by implants of mature lymphoid tissues in larval Xenopus laevisSupported in part by a grant (CA-08268) of the National Cancer Institute, National Institutes of Health, Bethesda, Maryland. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 457-465 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: One hundred and twenty-two larvae of Xenopus laevis, the South African clawed toad, at developmental stages 48, 50, 52 and 54, were implanted in the tail with two allografts from adult tissues. In each case, one allograft was from kidney, while the other was either from kidney, thymus, spleen, or liver. In any particular host the two implants were always from the same donor and the implants were all visually matched in size. The experimental period was a maximum of nine days, so as to minimize the large numbers of changes normally accompanying larval progress from stage to stage. We are concerned with the timing of allograft response initiation under the implant conditions of each experimental group at a particular point in development. An allograft response was defined as an infiltration and accumulation of small lymphocytes in the “test” kidney allograft. Larvae of all stages developed allograft responses within one week post-implantation when the variable implant was from kidney, but implants from spleen and thymus suppressed both the timing of initiation and the subsequent intensity of the response. Spleen was more effective in this regard than thymus and both were more effective in the earlier larval stages. Liver proved to be toxic to the larvae. The relationship between the maturation of the lymphomyeloid tissues and external morphological staging is also discussed.
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    URL: http://dx.doi.org/10.1002/jmor.1051380406
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  • 143
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    Fine structure of the midgut epithelium in the developing brown shrimp, Penaeus aztecus (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 467-485 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The midgut epithelium of larval and early postlarval brown shrimp has been studied with light and electron microscopy. Ultrastructurally the features of the midgut do not change during these stages of development. On the basis of electron density, two epithelial cell types can be distinguished, and these are referred to as light and dark cells. The dark cells contain more rough endoplasmic reticulum and more free ribosomes than the light cells. Mitochondria in the dark cells have a matrix which is less electron dense than the mitochondrial matrix of the light cells. Both cell types have a microvillous border with a surface coat. The microvilli lack microfilaments within their core, and a terminal web is not differentiated in the stages examined. Tubular smooth endoplasmic reticulum is abundant in the basal portions of the cells. Electron dense, membrane bound vesicles are consistently seen in association with the Golgi apparatus, apical cell surface, and gut lumen and therefore are believed to be secretory granules. Cells in the anterior portion of the midgut often contain very large lipid droplets in the cytoplasm.
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  • 144
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    Morphology of the nasal fossae and associated structures of the hamster (Mesocricetus auratus) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 161-179 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The hamster nasal cavity consists of vestibular, non-olfactory and olfactory portions. Much of the non-olfactory nasal cavity surface is lined by cuboidal, stratified cuboidal, and low columnar epithelia, devoid of cilia. Goblet cells and ciliated respiratory epithelium are present over only a small portion of the nasal cavity surface.The largest glandular masses in the hamster nose are the maxillary recess glands, the vomeronasal glands and the lateral nasal gland 1; these three glands contain neutral mucopolysaccharides (PAS-positive). Other nasal glands contain both acidic and neutral mucopolysaccharides; the staining reaction for acidic mucopolysaccharide is stronger in goblet cells and olfactory glands than in the other nasal glands.The ducts which open into the nasal vestibule are the excretory ducts of compound tubuloacinar serous glands. The one major PAS-positive gland whose duct opens into the nasal vestibule is the lateral nasal gland 1. The ducts of the compound tubuloacinar vomeronasal glands open into the lumen of the vomeronasal organ, which is connected to the ventral nasal meatus by means of the vomeronasal duct. The ducts of the branched tubuloacinar maxillary recess glands open into the maxillary recess. Few ducts open into the caudal half of the nasal cavity.
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  • 145
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    Granular secretion in the kidney sexual segments of female lizards, Cnemidophorus I. lemniscatus (Sauria, Teiidae) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 181-191 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A differentiated segment, analogous in location and structure to the first portion of the sexual segment of the males, but much smaller, is observed in the renal collecting ducts of female C. lemniscatus. In addition to this first portion, with cells full of granules strongly positive to periodic acidleucofuchsin, males have a consecutive second portion, with granules localized only in the apical part of the cells, moderately positive to the reaction mentioned and with a marked affinity for orange G. The two portions of the male sexual segment are considered to correspond to the middle and final parts of the collecting ducts; the initial part in both sexes and the final one in the female are mucigenous.
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  • 146
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    Patterns of regional variation in the vertebral column of terrestrial salamanders (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 257-277 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Regional variation in the vertebral column of several species of salamanders (families Ambystomatidae, Salamandridae and Plethodontidae) is analyzed. Measurements of three dimensions, centrum length, prezygapophyseal width, and transverse process length, provide the data. Ontogenetic, interspecific, intergeneric and interfamilial patterns of positional variation are diagrammed and discussed. Distinctive patterns of variation characterize the families, genera, and to a lesser extent, the species. The patterns of ambystomatid salamanders are the most generalized, and probably reflect derivation from a primitive ancestral stock. The most specialized conditions occur in the fully terrestrial plethodontids, a group generally considered to be highly derived. Data such as those presented here will aid in the identification of fossils.The patterns described have functional significance. For example, species which have an aquatic larval stage and which return to aquatic breeding sites have vertebrae which taper in length and width behind the pelvis. This is a feature associated with production of a traveling wave in the tail which is necessary for propulsion in water. Fully terrestrial species do not have a tapering column. In them, standing waves, such as occur in the trunk region of all species, typically occur in the tail. The caudal vertebrae of terrestrial species are rather uniform in dimensions for some distance, and the tail is cylindrical in form. Other functionally important features include the narrowing and shortening of some anterior vertebrae, associated with the development of a neck in some species with tongue feeding mechanisms. In contrast, species which use their heads as wedges during locomotion have broadened anterior vertebrae which serve as sites of origin for hypertrophied neck muscles.
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    The anatomy and histology of the nasal cavities and the nasal salt gland in four species of fringed-toed lizards, Acanthodactylus (Lacertidae)Dedicated to Prof. H. Mendelssohn on the occasion of his sixtieth birthday  -  “ad 120.”This paper is a part of a Ph.D. Thesis submitted to the Senate of the Hebrew University, Jerusalem. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 353-363 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The anatomy of the nasal cavities and lateral nasal gland (functioning as a salt gland) are similar in the four species of Acanthodactylus studies. Nasal cavities are not histologically different from those of other squamates. The vestibule is covered with a multilayered, stratified, squamous epithelium. The nasal gland is situated in the conchal space, lateral to the principal cavity and the vestibule. It is a tubular branched gland, highly vascularized. Innervation is derived from the N. trigeminus and was observed to reach only the anterior, small portion of the gland. The collecting duct opens to the vestibule, anterior to its connection to the principal cavity. Secretion of the nasal salt gland probably collects in lower anterior part of the vestibule. From there, liquid can either dry out while moistening the inspired air or be blown out by “sneezing.” Histological differences were observed between a non-active and an active salt gland.
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  • 148
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    The mechanism of ultraplanktonic entrapment in anuran larvae (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 279-287 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Tadpoles of several different genera were fed graded suspensions of uniform polystyrene particles to determine the lower size limit of particles that could be ingested. Certain tadpoles can extract suspended particles as small as 0.126 μ in diameter from the water. In terms of particle size, this is an efficiency comparable to the best mechanical sieves that can currently be produced by man. A mechanism for ultrasplanktonic entrapment is proposed on the basis of scanning electron micrographs of the secretory ridges in the branchial food traps of Rana catesbeiana before and after feeding.Xenopus tadpoles in yeast suspensions modify their clearance and buccal pumping rates in response to varying food concentrations. This may be an adaptation for maintaining a constant input of food mass to the tissues that extract the food from the water.Variability in the lower size limit of filterable particles among tadpoles of different genera correlates with the availability of suspended matter in the microhabitat where these tadpoles may be found.
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  • 149
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    Ultrastructure of the venom gland of the scorpion, Centruroides sculpturatus (Ewing) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 365-383 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The venom apparatus of the scorpion, C. sculpturatus (Ewing) was studied with light and electron microscopy. Each of the paired glands is lined by secretory epithelium made up of a single layer of columnar cells. Extensive folding in the epithelial layer creates a primitive acinar gland. The secretory products are either membrane-bound or unbound vesicles with discrete morphologies and are observed in the extruded venom, within the lumen of the gland, and within single secretory cells.The venom apparatus, including connective tissues, nerve cells, and muscle tunic is described and correlations are made with observations in other Athropods.
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    URL: http://dx.doi.org/10.1002/jmor.1051370307
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    A light microscopic study of the mononuclear cells infiltrating skin homografts in the garter snake, Thamnophis sirtalis (Reptilia: Colubridae)Submitted to the State University of New York, Upstate Medical Center, in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Supported by Training grant GM-00326 and Research grant HD-03484 from the National Institutes of Health. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 149-159 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The rejection of skin homografts in the snake, Thamnophis sirtalis is preceded by an infiltration of mononuclear cells into the graft bed. The initial arrangement of infiltrating cells in perivascular halos suggests that these cells emigrate from the blood stream of the host. A cytological study showed that the vast majority of the cells can be classified as small and mediumsized lymphocytes, monocytes and macrophages. Early stages of infiltration were associated with large proportions of lymphocytes while later stages were characterized by a predominance of macrophages. It was concluded that the mononuclear cells associated with graft rejection include large proportions of lymphocytes and macrophages and not just one kind of lymphoid cell.
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    Morphogenesis of intestinal villi. I. Scanning electron microscopy of the duodenal epithelium of the developing chick embryo (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 193-213 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Development of villi in the duodenum of the chick was studied in stages ranging from 11 days of incubation to one week after hatching. Formation of definitive villi is preceded by development of a set of previllous ridges that run lengthwise along the duodenum. The first set of 16 previllous ridges (Set I) is complete by about 13 days of incubation; all ridges in the set are fairly uniform and proceed through their subsequent development in synchrony. Previllous ridges in Set I fold into a highly regular zigzag pattern between 14 and 16 days of incubation. Definitive villi develop from Set I ridges beginning at about 17 days when populations of distinct cells appear on the crests of the ridges between angles in the zigzag folds. Cells in these populations lack the rounded appearance of cells seen in earlier stages; their apical surfaces are densely covered with microvilli. A second set of villi (Set II) develops at about 16 days of incubation when about 16 rows of tongue-like flaps erupt between the previllous ridges of Set I. At hatching, Set II villi are still smaller than villi of Set I; this distinction disappears by about the fourth day after hatching. The significance of the morphological changes in epithelial cells is discussed in terms of several hypotheses bearing on the mechanisms of villus formation.
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/jmor.1051370206
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    The relationship of early oocytes to putative neoblasts in the serpulid Spirorbis borealisWork supported by a grant (GB 18429) form N.S.F. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 215-227 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fine structure of the ovary in the serpulid Spirorbis borealis has been described. The ovarian wall consists of from one to several layers of peritoneal cells. Peritoneal cell processes extend deep into the ovary and may be seen between developing oocytes. Although young oocytes may also be in close apposition to one another, intercellular bridges have not been observed. When primary oocytes at the surface of the ovary reach a diameter of about 20 μ, they start to erupt into the coelom. Ovulation results from a simple separation of overlying peritoneal cells which lack specialized cell-to-cell contacts. Once a free surface of an ovulating oocyte is exposed to the coelom, microvilli and primary coat develop. Previtellogenic coelomic oocytes are often observed in close proximity to putative neoblasts (perivasal cells), which suggests a possible functional relationship. The confusion that extists between germ cells, peritoneal cells, and so-called neoblasts in polychaetes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051370207
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    A new model for the structure of the centriolar satellite complex in spermatozoa (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 229-241 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of the centriolar satellite complex in the spermatozoon of the hydroid, Pennaria, is presented. The complex consists of nine spoke-like arms which emanate from the distal centriole and of nine wedgeshaped connectives which extend into the flagellum joining each of the alpha doublets and the flagellar plasmalemma. Based upon these observations a new model for the structure of the centriolar satellite complex is proposed. In addition, similar centriolar satellite complexes are reported in the spermatozoa of two echinoderms, Ctenodiscus crispatus and Thyone briareus, in support of the proposed model.
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/jmor.1051370208
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  • 154
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051370301
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  • 155
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    Origin of mono- and binucleate cells with heterochromatic chromosomes in the malpighian tubules of the european elm scale, Gossyparia spuria (Coccoidea: Homoptera)Supported by grants GB 6745 and GB 23665 from the National Science Foundation, Washington, D.C. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 243-256 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Males of the European elm scale, Gossyparia spuria (Erioccoccidae) have two Malphigian tubules, each made up of mononucleate and binucleate cells. Both types of cells may contain heterochromatic (H) chromosomes which form an H body. The cells with H bodies (H cells) usually appeared singly anywhere along the tubule. However, when two or more H cells were present they tended to be closer to each other than would be expected by chance. The possible origin of this tendency is discussed. Following squashing, the nuclei of the binucleate cells were much larger than those of most other somatic cells, suggesting that they were highly endopolyploid. However, the H bodies of the cells of the tubules were of about the same size as those of the other cells. These observations suggested that the H chromosomes of the binucleate cells did not replicate while the euchromatic chromosomes of these cells replicated several times. The great majority of the nuclei of the H cells contained a single H body per nucleus. An analysis of the number of H bodies in binucleate cells indicated that when two H bodies were present in the same nucleus they usually did not fuse. Thus, they were believed also not to fuse in the mononucleate cells. Since almost all the mononucleate H cells had only a single H body (rather than 2) it was concluded that they did not originate from binucleate cells by nuclear fusion.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051370209
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    Digit flexor muscles in the cat: Their action and motor unitsSupported in part by USPHS grants NB 07888 and FR 05457. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 335-352 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The relation between muscle action and the mechanical properties of motor units has been explored in the main digit flexors of the cat hind limb: plantaris (PL); flexor digitorum brevis (FDB); flexor hallucis longus (FHL); and, flexor digitorum longus (FDL). General observations on muscle action revealed that PL is an ankle extensor as well as a digit flexor. PL and FHL were shown to be the major force contributors to digit flexion with FDL playing a lesser but still significant role.The mechanical properties of PL, FHL and FDB motor units were studied by noting twitch and tetanic tensions produced by electrical stimulation of single alpha axons, functionally isolated from the ventral root filaments. These data were compared to similar data reported by Olson and Swett (1966) for flexor digitorum longus (FDL). Our sample (114 PL, 60 FDB and 124 FHL units) disclosed that PL, FDB and FHL have units of uniformly fast contraction times (means 22, 27 and 27 msec respectively). PL units developed the most tetanic tension (3 to 160, mean 62 gm-wt) followed by FHL (2 to 87, mean 31 gm-wt) with FDB units producing very little tension (1 to 20, mean 6 gm-wt). Swett and Olson's FDL sample (108 units) showed tensions ranging from 0.3 to 100 gm-wt (mean 10 gm-wt).A division of labor among the four muscles is proposed. The large PL units are advantageous for forceful phasic inputs to the digits during the locomotion and in keeping with PL's additional role as an ankle exstensor. The low output forces of FDB units are optimal for discrete input to the digits during subtle adjustments of posture. We propose that the larger fast contracting units of FHL are used primarily for forceful digit flexions required in locomotion and for phasic protrusion of the claws while the predominately small and slow contracting units of FDL are used for sustained claw protrusion.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051370305
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    The development of the chondrocranium of the lacertid lizard, Acanthodactylus boskiana (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 289-333 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The trabeculae cranii are at first quite separate from each other, after few days their anterior two fifths are connected by a trabecular plate which is obliterated throughout development. The paired origin of the parachordal plate is not observed. The fused posterior orbital cartilages chondrify in the form of a wide short plate, traversed by the oculomotor and trochlear nerves. The basicranial fenestra and fenestra ovalis are formed by the degeneration of pre-existing cartilage. The cochlear portion is completely fused with the parachordal plate from the very beginning. The elements of the pterygoquadrate are fused together. The quadrate and Meckel's cartilage are in close contact from the very beginning. While the lower part of the interorbital septum is derived from the trabecula communis, its upper part is derived from the anterior orbital cartilages. The lateral parts of the fused posterior orbital cartilages give rise to most of the taeniae and pilae of the orbitotemporal region. There is only one commissure between the auditory capsule and parachordal plate. A cartilaginous connection between the distal portion of the columella auris and ceratohyal persists for some time. The parietotectal and paranasal cartilages are fused together from the very beginning. The processus paroticus originates from the columella auris.In the fully formed stage the notochord is completely embedded in the occipital condyle. The union between the condyle and odontoid process persists. The auditory capsules and occipital arches contribute to the formation of the tectum synoticum plus posterius. The prefacial commissure and facial foramen lie in front of the cochlear portion. The columella auris possesses a processus internus (connected with the quadrate), but the processes a dorsalis has completely disappeared. The orbitotemporal region is quite complete. A medial fenestra is formed in the planum supraseptale. A fenestra is observed in each of the interorbital and nasal septa. The lamina transversalis anterior is fused with the parietotectal cartilage. A complete zona annularis is present. The outer wall of the paranasal cartilage is perforated by a large fenestra lateralis. The parietotectal and paranasal cartilages and the posterior process of the lamina transversalis anterior contribute to the formation of the concha nasalis. There is a contact between the planum antorbitale and nasal septum. The pterygoid process has disappeared. The common characters of the lacertid chondrocranuium are deduced.
    Additional Material: 22 Ill.
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051370401
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    A cytological study of the ovary of Rhodnius prolixus. II. Oocyte differentiation (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 385-415 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This investigation presents the ultrastructural changes that occur during previtellogenic and vitellogenic growth of the oocyte of a telotrophic ovary. The ovarioles of a newly emerged adult Rhodnius have their full complement of oocytes located at the base of the tropharium. These oocytes are in cytoplasmic continuity with the syncytial tropharium via trophic cords. The nuclei of these oocytes are in prophase of meiosis I with condensed chromosoes; synaptonemal complexes are visible in many. During oocyte growth the nucleus or germinal vesicle enlarges from a 3-4 μ diameter to a 45-50 μ diameter and the chromosomes become diffuse and arrested in late prophase I. Small previtellogenic oocytes are limited by a morphologically unspecialized oolemma and contain a pair of centrioles, rough endoplasmic reticulum, a few small Golgi complexes and clusters of mitochondria. By the end of previtellogenesis there has been an increase in the volume of the oocyte due to the transport of ribosomes and mitochondria into the oocyte from the tropharium. During vitellogenesis the oolemma develops a microvillous border and yolk precursors are internalized by pinocytosis. Small stacks of annulate lamellae, numerous ribosomes and the other organelles are restricted to the thin layer of cortical ooplasm; the lipid protein-carbohydrate yolk spheres and glycogen are located centrally. In oocytes not stimulated, autolysis occurs as dense local areas within the ooplasm.
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    URL: http://dx.doi.org/10.1002/jmor.1051370402
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  • 160
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    Analysis of the developing avian glycogen body. I. Ultrastructural morphology (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 463-481 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An electron microscopic study of several stages in the development of the glycogen body of the chick is reported. During early developmental stages (7.5-11 days) glycogen granules were scattered throughout the cytoplasm of glycogen-body cells. Lysosomes were observed for the first time on the eleventh day of incubation. In the course of subsequent development three well demarcated cytoplasmic areas became recognizable; a juxtanuclear region that contained most of the organelles and was devoid of glycogen, a peripheral area (ectoplasm) free of glycogen, and a region densely packed with glycogen. Cells that were heavily laden with glycogen contained unusual rounded or “C”-shaped multivesicular bodies which were associated with the Golgi complex and with Golgi-like vesicles. Their functional significance did not become evident in this descriptive study.The paucity of smooth endoplasmic reticulum indicated its lack of importance in glycogen metabolism in the glycogen body. Ribosomes, which were the only organelles consistently observed in close proximity to glycogen granules, were thus implicated in glycogen synthesis.
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    URL: http://dx.doi.org/10.1002/jmor.1051370406
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  • 161
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    Nucleolar organization in oocytes of gryllid crickets: Subfamilies gryllinae and nemobiinae (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 433-447 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A cytological and cytochemical survey was made of nucleolar changes during oocyte development in several different species of crickets (Gryllidae) representing the subfamilies Gryllinae and Nemobiinae. A large mass of extrachromosomal DNA is characteristic of the pachytene stage nuclei of all species examined. Nucleolar material accumulates at the periphery of the DNA body as the cells proceed into the diplotene stage of development. As the oocytes proceed through diplotene, the nucleoli reorganize into many small masses which eventually disperse in the nucleoplasm. These changes reflect both an increase in number and in size of the nucleolar material during the diplotene stage and the mode by which dispersal of nucleolar material is accomplished. These differences probably reflect differences in the organization of extrachromosomal nucleolar DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051370404
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    Effects of hypophysectomies and thyroxine replacement upon the initiation of tail regeneration in the lizard, Anolis carolinensisSupported by grants to M. Singer from the American Cancer Society, the National Institutes of Health and the Multiple Sclerosis Society. (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 449-461 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A histological evaluation of the effects of hypophysectomy and throxine therapy in young tail regenerates was carried out in the small iguanid lizard, Anolis c. carolinensis. Hypophysectomy caused a delay but did not inhibit blastema formation. The growth of the ependyma into the wound region was delayed in hypophysioprivic regenerates by about a week.Growth and differentiation of hypophysioprivic regenerates after blastema formation was variable, ranging from virtually no growth to the formation of a differentiated but very small protuberance. However, actual tail elongation was inhibited by hypophysectomy. In those hypophysioprivic regenerates that did show signs of differentiation, muscle groups were poorly defined, scanty in appearance and not as well differentiated as the cartilage tube.Thyroxine treatment in the young hypophysioprivic regenerates stimulated normal growth and normal appearance and differentiation of promuscle and procartilage aggregates as well as the growth of the ependymal tube into the blastema.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051370405
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  • 163
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    DNA synthesis, mitosis and fusion of myocardial cells (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 417-431 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Myocardial cells obtained from embryonic chick ventricles have been used to investigate (1) whether differentiated cells can undergo DNA synthesis and mitosis and, (2) whether heart cells when grown in culture can fuse with each other and with chick skeletal myoblasts to form heterokaryon myotubes. Electron microscopic observations have shown that myocardial cells of day 3 and day 20 chick embryos did contain myofibrils with defined sarcomeres; these cells have been observed in mitosis. Cells obtained by tryptic digestion of day 12 chick ventricles when grown in culture continued to replicate their DNA as shown by thymidine-3H radioautography with DNase controls and were observed in all stages of mitosis. Electron microscopy showed that myofibrils were present in some of the cultured cells.Bi-, tri- and tetranucleate cells were observed in the cultures. Thymidine-3H radioautography showed that these cells were formed by karyokinesis without cytokinesis and by the fusion of uninucleate cells. Since the heart cells could fuse with each other, we tested the possibility that they could fuse with skeletal myoblasts to form heterokaryon myotubes. This was accomplished by co-culturing thymidine-3H labeled ventricular cells and unlabeled skeletal myoblasts. Radioautography with DNase controls showed that some of the myotubes consisted of unlabeled skeletal muscle nuclei and labeled heart nuclei in varied proportions. The factors initiating the formation of these heterokaryons have not been elucidated.
    Additional Material: 11 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051370403
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    A light and electron microscopical study of spermatogenesis in Hydra cauliculata (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 137 (1972), S. 483-501 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Morphological changes in the interstitial cells were studied during their differentiation into spermatozoa. Development of the spermatogonium involves an increase in nuclear and nucleolar size, and the formation of a dense mass of cytoplasmic ribosomes. The mature spermatozoon has a relatively simple structure. The head consists of a bullet shaped, homogeneous nucleus, which lacks an acrosome but bears distal membrane specializations. The middle piece is composed of four large spherical mitochondria at the base of nucleus. A single flagellum projects from one of the two centrioles lodged between the mitochondria. The flagellum appears early during development in the primary spermatocyte. During spermiogenesis microtubules associated with the basal body flagellum complex appear to define the axis of chromatin condensation.
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    URL: http://dx.doi.org/10.1002/jmor.1051370407
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  • 165
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051380101
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  • 166
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    Masthead (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051380201
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    The fate of the normal-anucleated spermatozoa in inseminated females of the silkworm Bombyx mori (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 121-129 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Both typical (haploid) and atypical (anucleated) spermatozoa reach the receptaculum seminis of inseminated females of Bombyx mori intermingled. However, only typical spermatozoa both leave the receptaculum and fertilize the eggs. Atypical spermatozoa, which are in fact anucleated flagellar apparatuses, probably function in transporting typical fertilizing spermatozoa to the receptaculum seminis. In the male ejaculatory duct both kinds of spermatozoa are wrapped with extra-cellular sleeves that presumably protect them on their way to the receptaculum. Typical spermatozoa “hatch” from the sleeves before leaving the receptaculum to fertilize the eggs. The presence of a centriole in the extra-testicular spermatozoa of this species supports the generalization that insect spermatozoa do have a centriole at the base of the flagellum.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1051380104
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  • 168
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    A cytological study of the ovary of Rhodnius prolixus. III. Cytoarchitecture and development of the trophic chamber (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 138 (1972), S. 1-39 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The tropharium of the telotrophic ovarioles of Rhodnius is syncytial with the nurse cell nuclei located in tortuous finger-like projections arborizing from a common cytoplasmic area, the trophic core. The nurse cell nuclei exhibit prominent nucleoli. Located adjacent to the nuclear envelope are masses of granular material both within the nucleus and adjoining cytoplasm. The cytoplasm consists primarily of ribosomes and mitochondria. The trophic core and the trophic cords that connect the core to individual oocytes characteristically possess parallel arrays of microtubules with ribosomes and mitochondria interspersed between. Surrounding the nurse tissue (germarium) is a thin layer of squamous cells comprising the inner sheath. The inner sheath is encompassed by the non-cellular tunica propria superficial to which are two external cellular sheaths.The syncytial nature of the tropharium appears to arise as a result of the fusion of many entangled nurse cell-oocyte complexes during the late fifth instar. The structural similarities, and possible homologies with the polytrophic type of ovariole is discussed.
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    URL: http://dx.doi.org/10.1002/jmor.1051380102
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    Bacteria which attack petroleum hydrocarbons in a saline medium (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 319-330 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacterial strains were isolated from California coastal areas which showed the ability to oxidize normal paraffins, iso-paraffins, and aromatic hydrocarbons in a synthetic seawater medium. The ability to utilize a particular hydrocarbon was established not only on the basis of visible bacterial growth but also through a chromatographic technique which was standardized and which could define the amount of each hydrocarbon consumed by the bacteria in a mixture. Some of the strains exhibited vigorous hydrocarbon oxidation when exposed to synthetic mixtures of hydrocarbons as well as crude oil. Under conditions of aeration and agitation, mixed cultures could destroy approximately 50% of a South Louisiana crude oil in a period of 48 hr.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260140305
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  • 170
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    Measurements on the interfacial areas of hydrocarbon in yeast fermentations and relationships to specific growth rates (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 345-360 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The transport of insoluble substrates such as hydrocarbons to microorganisms is often postulated to be dictated by the availability of the hydrocarbon surface area. Many publications, qualitative and quantitative, have appeared to substantiate this hypothesis. Experiments have been performed in our laboratory to assess the absolute values of the interfacial area of hexadecane as the carbon source for the growth of Candida intermedia. A sedimentometer, mounted directly in the fermentor, was used to measure the interfacial hydrocarbon area during active growth of this organism. The specific hydrocarbon interfacial area was found to be directly related to the impeller speed, hydrocarbon concentration and surfactant concentration in a 1-liter working volume, turbine-agitated fermentor. The specific growth rate was in turn found to be directly related to the specific hydrocarbon interfacial area. Lastly, cessation of logarithmic growth and onset of linear growth was found at all instances to be governed by the specific hydrocarbon surface area.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260140307
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    Effects of growth rate and influent substrate concentration on effluent quality from chemostats containing bacteria in pure and mixed culture (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 391-410 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies were performed using pure cultures of A. acrogenes and E. coli and a heterogeneous microbial population growing in carbon-limited chemostats with glucose as the sole carbon and energy source. A two-level factorial experimental design was employed to test the hypothesis that the concentration of growth-limiting substrate in a chemostat is controlled by the growth rate alone and is independent of the concentration of substrate entering the reactor. The pure culture experiments showed that the conclusions depend upon the measurement employed for growth-limiting substrate. When the concentration of glucose was measured directly, the hypothesis was found to be true within the limits of the study (500-1500 mg/liter). However, if the chemical oxygen demand (COD) test was used as the measure of growth-limiting substrate the hypothesis was found to be false. When heterogeneous cultures were employed the hypothesis was false regardless of the technique used to measure the concentration of growth-limiting substrate. Nevertheless, it was possible to generate regression equations which described the interactions among influent COD, growth rate, and effluent COD with a high level of correlation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140310
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    Thermal inactivation of immobilized enzymes in ideal continuous reactors (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 473-491 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The limitation of thermal inactivation on catalytic activity in continuous enzymatic reactions is considered. Where an enzyme is retained in a reaction environment which is open to mass transfer of reaction components, the effect of enzyme inactivation on reactant conversion depends on the order of the chemical reaction and the pattern of fluid flow through the reaction volume. Equations expressing conversion as a function of time for first-order inactivation are presented for Michaelis-Menten kinetics and the limiting fluid flow conditions of plug flow and complete back-mixing. Substrate protection or destruction of an enzyme is also considered and it is shown theoretically that the catalytic life of an enzyme may be optimized by the proper choice of fluid flow pattern.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140315
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  • 173
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    Masthead (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140201
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  • 174
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    Inactivation of immobilized catalase by hydrogen peroxide in continuous reactors (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 201-205 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is shown theoretically that in continuous reactions the rate of catalase inactivation by hydrogen peroxide depends on the type of reactor and the order of the chemical reaction.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140205
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  • 175
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    Degradation and mineralization of petroleum by two bacteria isolated from coastal waters (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 297-308 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Within the framework of a study on the oil biodegradation potential of the sea the ability of a Flavobacterium sp. and Brevibacterium sp. to metabolize a paraffinic crude oil and a chemically defined hydrocarbon mixture was investigated. Major components of the crude oil were identified by combination gas chromatography and mass spectrometry. The rate and extent of total hydrocarbon biodegradation was measured. In addition, CO2 evolution from the crude oil was continuously monitored in a shaker-mounted gas train arrangement. Degradation started after a 2 to 4 day lag period, and reached its maximum within two weeks. At this time up to 60% of the crude oil and 75% of the model hydrocarbon mixture, each added at the level of 1 ml per 100 ml artificial sea water, were degraded. Mineralization(conversion to CO2) was slightly lower due to formation of products and bacterial cell material. n-Paraffins were preferentially degraded as compared to branched chain hydrocarbons. Biodegradation of n-paraffins in the range of C12 to C20 was simultaneous; no diauxie effects were observed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140303
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  • 176
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    Flocculant production from kerosene (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 379-390 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cultivation of Corynebacterium hydrocarboclastus, which is capable of synthesizing an extracellular polymer and utilized hydrocarbons, has been reported. Growth studies in shake flasks and fermenters were made to obtain maximum polymer production. Polymer formation was found to be growth associated. The highest level of polymer accumulation was attained after 50-60 hr cultivation in the fermenter and it amounted to approximately 5.5-6 g/liter of fermentation broth. The medium contained initially 2% (v/v) kerosene as a carbon source. The maximum yield obtained corresponds to 37-40% (w/w) of kerosene supplied. At the same time the cell concentration was 10-13 g/liter which represents the yield of 67-87% (w/w). The rate of polymer production in the exponential phase was 0.25 g/liter hr and cell production rate was 0.27 g/liter hr. Sodium nitrate, 0.5%, and yeast extract, 0.3%, (w/w) were the best nigrogen sources for polymer formation. The highest level of polymer produced in broth was 6 g/liter.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140309
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  • 177
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    Large scale synthesis of dinucleoside monophosphates catalyzed by ribonuclease from Aspergillus clavatus (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 861-870 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A gram scale enzymatic synthesis of eight, dinucleoside monophosphates (ApC, ApU, CpC, CpU, GpC, GpU, UpC, and UpU) is described. The synthesis involves a reaction between the appropriate ribonucleoside-2′,3′-cyclie phosphates and cytidine or uridine in the presence of ribonuelease from Aspergillus clavatus at 30°C. The enzyme is removed from the reaction mixture by chromatography on Bio-Gel P-4, and the dinucleoside monophosphate is further purified by chromatography on a DEAE-Sephadex A-25, column.A procedure for the large scale preparation of the ribonuclease from Aspergillus clavatus is also described.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140602
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  • 178
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    Conversion of deteriorated metal cutting fluids into protein (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 361-377 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Six soil isolates were grown on a petroleum sulfonate medium and analyzed chromatographically to determine their amino acid composition. The organisms were also examined to determine their growth rate in the petroleum medium, their per cent conversion of hydrocarbon to dry cell mass, their protein content, and their possible use as a food or food supplement. It was found that the isolates varied extensively as to their amino acid composition. Several of the isolates proved to convert the hydrocarbons to cell mass with relative ease. The proteins of four of the isolates appear to be suitable as a good food source, and the protein of one of the remaining isolates appears to be a very good food supplement.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140308
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  • 179
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    Rubella virus replication at controlled dissolved oxygen tension (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 493-497 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140316
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  • 180
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    Glucose isomerase covalently bound to porous glass beadsPresented at 5th Great Lakes Regional American Chemical Society Meeting, Peoria, Illinois, June 10-11, 1971 (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 509-513 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140319
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  • 181
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    Growth models of cultures with two liquid phases. VII. Substrate dissolved in dispersed phase; effect of dispersed phase volume and temperature (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 571-586 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of dispersed phase volume and temperature on the batch growth of Candida lipolytica on gas oil are investigated. Growth parameters are presented for two sets of experiments. The shape of growth curves was basically similar to the system composed of n-hexadecane dissolved in dewaxed gas oil, in spite of the complex nature of the substance. All of the batch growth curves exhibited a linear growth region. The rate of linear growth and its length varied with change in dispersed phase volume. The effect of temperature on growth rate was investigated for temperatures ranging from 23°C to 34°C. The results show a smaller activation energy during linear growth than during the early stages of batch growth. These results are analyzed from the viewpoint of growth models presented previously. The results indicate that growth at drop surfaces is important and that segregation effects may be important.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140404
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  • 182
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    Multiple junctions in the pipework of pilot plant fermenters (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 679-681 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140413
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  • 183
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    Dynamics and control of continuous microbial propagators to subject substrate inhibitionPresented in part at the 160th Annual Meeting of the American Chemical Society, September, 1970, Chicago and in part at the 63rd Annual Meeting of the American Institute of Chemical Engineers, November, 1970, Chicago. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 939-974 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Inhibitory substrate levels are common in industrial fermentations and in biological waste-water treatment of many industrial wastes. Continuous microbial cultures are unstable to certain disturbances, such as shock loading by inhibitory substrates. Two feedback proportional control strategies are analyzed and compared for a simple model culture assumed represent able by the culture concentrations of biomass and a single rate-limiting and growth-limiting nutrient (substrate). One control strategy, the well known turbidostat, consists of adjusting culture holding time (e.g., by flow rate adjustment) in response to deviations in turbidity or some other measure of culture biomass concentration. The other control strategy is to adjust holding time in response to deviations in limiting nutrient concentrations in the culture. This second control strategy, termed the nutristat, can be superior to the turbidostat in many applications. The sign and magnitude of the dimensionless group {(X/YD)[dμ/dS]s}, is shown to be an important determinant, in the behavior of the open loop and the two closed loop processes. This characteristic group is positive when the specific growth rate is increased by increases in the nutrient concentration, zero when the growth rate is unaffected by the nutrient concentration, and negative in the presence of nutrient or substrate inhibition.The effects of process modifications and of modeling assumptions on the control of the process are discussed and more sophisticated control schemes are also proposed.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140606
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  • 184
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    Quantitative studies of the development of S. cerevisiae mitochondria (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 1007-1026 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The quantitative changes in mitochondria and cytochromes during transition of Saccharomyces cerevisiae from one steady state to another, while growing in continuous culture under controlled environmental conditions, were followed.No Mitochondria, or mitochondria like structures, were detectable in electron micrographs of permanganate-fixed anaerobic cells. Microaerobiosis (3μM dissolved oxygen) was sufficient to visualize mitochondrial profiles and induce cytochromes and their sections had a reduced number of mitochondrial profiles compared with cells grown in limiting glucose.In the presence of ergosterol and Tween 80 mitochondriogenesis, whether induced by aerobiosis or glucose limitation, involved enhanced definition of crystal and outer mitochondrial membranes and increased number of profiles. Where membrane formation was limited, by the absence of aerobiosis involved eytochrome induction and profile visualization, but limited profile Proliferation; the adapted cells consequently contained fewer, but more eytochrome-enriched, mitochondria than cells adapted in the presence of ergosterol and Tween 80.Increase in dissolved oxygen from 3μM to 52μM further enhanced membrane definition and increased the size, but not the number, of mitochondrial profiles.Evidence, obtained by measurement of eytochrome concentration per unit mitochondrial volume and per unit crystal area, support the concept that mitochondriogensis and cytochrome synthesis are not synchronized process and that cytochromes are added to or depleted from the mitochondrial cristae in response to culture conditions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140609
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  • 185
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    Symbiotic growth of Acetobacter suboxydans and Saccharomyces carlsbergensis in a chemostat (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 75-92 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The bacterium Acetobacter suboyxdans and the yeast Saccharomyces carlsbergensis have been grown together on a synthetic medium in a chemostat. Mannitol, the only carbon source fed to the fermenter, is oxidized by the bacteria to fructose. The yeast, which cannot attack mannitol, breaks down the fructose nearly completely. Eight steady states and five transitory periods after changes in flow rate have been analyzed to study the kinetics of the mixed culture. Separate cell concentrations were determined by a modified Coulter counter apparatus. Both sugars were monitored. Both bacteria and yeast may be modeled using Monod's equation, the latter with some deviations. The yeast is unable to grow beyond the washout point of the bacteria, even though its maximum growth rate is much higher. The yield of both organisms decreases with increasing dilution rate, as does their average cell size. After step changes in dilution rate, repeated oscillations of both sugar and cell concentrations usually occur before steady-state conditions are reattained. They are generally in phase, with no definite sign of a lag. Oscillations of yeast and fructose concentrations are more pronounced. Periods average about 6 hr and are not correlated with fermentation conditions or equipment variables. Repeated oscillations are not found after step-downs in pure cultures of A. suboxydans, leading to the conclusion that the instability in mixed cultures may be caused by a feedback mechanism from the yeast to the bacteria.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140108
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  • 186
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    Continuous culture of Pseudomonas fluorescens with sodium maleate as a carbon sourcePresented in part before the Annual Meeting of the Division of Microbial Chemistry and Technology, American Chemical Society, Chicago, Illinois, September, 1970. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 123-147 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pseudomonas fluorescens (ATCC 11150) was grown in batch and continuous culture in minimal media with sodium maleate as growth-limiting sole organic carbon source. Growth was followed by turbidity and dry weight measurements. Gross composition of washed cells (relative amounts of protein, lipid, RNA, and DNA) and the distribution of amino acids in protein hydrolyses of the cells were determined for cells grown in continuous culture at various dilution rates. Extracellular concentrations of the original carbon source and a number of metabolites were monitored by a total carbon analysis, ion exchange chromatography, and ultraviolet-visible scans of cell-free supernatants and chromatographic fractions, thereof.Substrate inhibition by maleate was a major factor in the growth kinetics of both batch and continuous cultures. Excessive maleate concentration caused instability in continuous cultures. By appropriate operation, much higher specific growth rates (0.305/hr) could ultimately be achieved in continuous culture compared to batch culture (0.174/hr). Adaptation was responsible for only part of the differences between batch and continuous cultures; the differing distribution of metabolites were also major factors.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140111
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  • 187
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    Kinetic behavior of mixed populations of activated sludgePresented at the Symposium on Applications of Continuous Culture Theory to Biological Waste Water Treatment Processes, 162nd National American Chemical Society Meeting, Washington, D.C., September 13, 1971. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 179-199 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetic behavior of heterogeneous microbial populations of sewage origin was studied in a single-stage isothermal continuous flow completely mixed aeration tank. A series of experiments were carried out at various dilution rates using glucose as the growth limiting substrate. The steady-state behavior of the system was observed at each dilution rate and the results were found to fit fairly well with the steady-state equation bayed on the Monod model with an endogenous respiration term included, i.e., μ = μmS/(Ks + S) - Kd. The growth kinetics of cells harvested at steady state for each dilution rate were studied using batch experiments. The multiple response data of the system as functions of time were used to estimate the parameter values in the above kinetic model. It was found that values of the growth parameters changed significantly and systematically with cell population. For example, values of μm were high at high dilution rates and low at low dilution rates. It was also found that only those batch growth parameters from cells obtained at fairly high dilution rates are comparable with those estimated by the results of steady-state operations. The results of this investigation suggest that (1) different cell populations pre dominated at different steady-state dilution rates, with high dilution rates resulting in predominantly fast-growing organisms and low dilution rates resulting in predominantly slow-growing cells, and (2) risk exists in any randomly picked batch experiment to predict the steady-state behavior of the system when heterogeneous microbial populations must be used.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140204
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  • 188
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    Kinetic model identification in mixed populations using continuous culture data (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 207-231 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetic behavior of heterogeneous microbial populations of sewage origin was studied in a single-stage, isothermal, continuous flow, completely mixed aeration tank. A series of experiments were carried out at various dilutions rates using glucose as the limiting substrate. The cell dry weight and substrate concentration in terms of chemical oxygen demand (COD) were continuously monitored. The results indicate that reproducible steady-state conditions can generally be obtained; however, multiple steady states were observed at dilution rates near washout. At low dilution rates (below about 0.1 hr-1) the contribution of microorganism decay became appreciable. Using the multiresponse data of cell dry weight and COD, the parameter values in various existing growth models were estimated. The analyses of variance and residuals revealed that models proposed by Moser, Monod, and Contois, each with a decay term added, were significantly better than the other models which were tested.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140206
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  • 189
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    Kinetic studies of the lactic acid fermentation in batch and continuous culturesPresented at 162nd National American Chemical Society meeting, Division of Microbial Chemistry and Technology, Washington, D.C., September 14, 1971. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 233-252 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The fermentation kinetics of the homofermentative organism Lactobacillus delbrueckii in a glucose-yeast extract medium is studied in both batch and continuous culture under conditions of controlled pH. From a graphical analysis of the batch data, a mathematical model of the process is derived which relates bacterial growth, glucose utilization, and lactic acid formation. The parameters in the model represent the activity of the organism and are a function of pH, having a maximum value at about 5.90. In a continuous stirred tank fermentor (CSTF), the effect of pH, feed concentration, and residence time is observed. The feed medium is a constant ratio of two parts glucose to one part yeast extract plus added mineral salts. An approximate prediction of the steady-state behavior of the CSTF can be made using a method based on the kinetic model derived for the batch case. In making step changes from one steady state to another, the transient response is observed. Using the kinetic model to simulate the transient period, the calculated behavior qualitatively predicts the observed response.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140207
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  • 190
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    Effect of wall growth on scale-up problems and dynamic operating characteristics of the biological reactor (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 253-265 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: If inhibitory substrates are being utilized in a well-stirred biological reactor, microbiological growth on the walls of the reactor can create a scale-up problem. A simple model is proposed which shows that without such growth, of the three existing steady states only one is stable and nontrivial, but with wall growth the trivial, stable, steady state (washout) is impossible. In addition, wall growth reduces the region over which three steady states are feasible and reduces the minimum residence time for which there is only one steady state that corresponds to a high conversion. Thus, a laboratory process with a high surface area to volume ratio can give an over optimistic prediction of both necessary residence; time and stability of the full scale process unless wall growth is accounted for.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140208
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  • 191
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    Biotechnology report: Solid state fermentationsPresented at the 1st International Mycological Congress, Exeter, England, September 7-16, 1971. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 517-532 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A unique method is described by which large yields of secondary metabolites arc produced on solid substrates. The process involves the use of moist substrates which are continuously agitated in appropriate fermentation equipment. The amount of agitation, aeration, and moisture can be varied. Extremely high yields of secondary metabolites such as ochratoxin and aflatoxin were obtained using Aspergillus and Penicillium species. The process prevents sporulation of the fungus and because of the nature of the solid substrate makes recovery of the product easier than in conventional liquid media. The substrates include rice, corn, wheat, and other cereals.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140402
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  • 192
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    Measurement of yeast concentrations in liquid hydrocarbon fermentations: Influence of experimental conditions and statistical significance of the results obtained (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 609-615 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of the following factors on the measurement of yeast concentration in liquid hydrocarbon fermentations was studied: surfactant, type (Tween 20, Tween 80, and G 3300), yeast concentration (0.215 to 0.272 and 3.37 to 4.08 g/liter, as dry matter), oil concentration (8.32 and 61.6 g/liter), surfactant, concentration (0.515, 1.030, 1.545, and 2.060 g/liter), and time of contact of the surfactant with the oil-yeast-aqueous medium mixture (2 and 10 min). The statistical significance of the obtained results was determined.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140406
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  • 193
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    External diffusional resistance in immobilized-enzyme catalysis (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 675-678 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140412
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  • 194
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    Masthead (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140501
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  • 195
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    Electronic Resource
    The “rotary column” method for growth of large-scale quantities of cell monolayers (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 753-775 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method and an apparatus that reduce the heavy expenses involved in traditional methods for the large-scale monolayer production of primary and secondary, strain and line cells and of the biologicals derived therefrom are described. The method is based on the principle of gathering in a single unit a sheaf of columns by means of general manifolds fitted with cocks. The growth of cells on the glass walls is irrespective of the number, length, and diameter of the columns used. The apparatus, placed in a thermo-adjustable room and connected to adequate devices which allow it to rotate on its longitudinal axis and to be set in a vertical position, need not be dismounted nor transported since it can be connected by a number of tubes to the necessary services. Sterilization is carried out by flowing steam and fluids are poured in or drained off by vacuum or pressure. A microscope fitted to the bearing structure allows the operator to observe the cell monolayers and the cytopathic effect of viruses on the whole length of the outer columns. During the various working stages pH is under continuous control and automatically adjusted. The whole working cycle is extensively described (cleaning, sterilization, seeding, incubation, trypsinization of the monolayer, culture and harvesting of the virus) and results compared with those obtained by traditional methods.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140506
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  • 196
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    Electronic Resource
    Model for the growth of aerobic microorganisms under oxygen limiting conditions (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 787-798 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple dynamic model is proposed which will allow fermenters to be run at throughputs which fully utilize the mass transfer capabilities of the fermenters while not decreasing the yield from the substrate. The model is compared with one previously proposed, which was originally formulated for double substrate limitation when both substrates were supplied in the feed. Computer solutions of the model are given which show the effects of the parameters used. Experimental results from growing Candida utilis on a high concentration of glucose were found to be similar to those predicted by the model.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140508
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  • 197
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    The fermentation of L-sorbose by Gluconobacter melanogenus. II. Inducible formation of enzyme catalyzing conversion of L-sorbose to 2-keto-L-gulonic acid (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 811-818 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cell-free extracts of Gluconobacter melanogenus cells grown in L-sorbose-containing media contained an enzyme system capable of converting L-sorbose to 2-keto-L-gulonic acid while cells grown in glycerol media did not. This inducible enzyme was located in the participate fraction of the cells.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140510
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  • 198
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    Electronic Resource
    Kinetic studies of C. pyrenoidosa using 94% 13C CO2 (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 819-829 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The alga Chlorella pyrenoidosa has been grown in mass quantities on 94 at. % 13CO2. The algal cells have been labeled to the 90 at. % 13C level. Neither inhibition nor a requirement for adaptation was encountered; changes in morphology were not evident. A statistically significant increase in mass of cells produced in the presence of 13CO2 was observed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140511
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  • 199
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    Electronic Resource
    Induction of linear growth in candida utills by temporary cessation of growth (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 851-855 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140515
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  • 200
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    Electronic Resource
    Open tubular heterogeneous enzyme reactors: Preparation and kinetic behavior (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 885-914 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Tubes with immobilized enzymes on the inner wall, called open tubular heterogeneous enzyme reactors, were prepared by binding enzymes either directly to the tube inside surface or to a layer of a porous matrix attached to the inner wall. Kinetic studies of the hydrolysis of N-benzoyl-L-arginine ethylester as a model reaction indicated that the reaction was kinetically controlled in reactors with surface bound trypsin and the kinetic parameters were evaluated by conventional methods. On the other hand, substrate diffusion in both the porous matrix and the bulk substrate solution strongly affected the rate of reaction in porous layer trypsin reactors. The highest overall rates of reaction were obtained when the reaction was bulk diffusion controlled and the measured rates were in agreement with those calculated from expressions derived from heat transfer theory. The design of reactors for the limiting cases of kinetic and bulk diffusion controlled reaction as well as a method for the determination of substrate diffusivity are outlined.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140604
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