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  • 1995-1999  (46)
  • 1980-1984
  • 1997  (46)
  • Ultrastructure
  • Nuclear reactions
  • 1
    Electronic Resource
    Electronic Resource
    Waxy corn starch affecting texture and ultrastructure of sardine surimi gels (1997)
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 204 (1997), S. 121-128 
    Details
    ISSN: 1431-4630
    Keywords: Key words Surimi ; Sardine ; Starch ; Texture ; Water holding capacity ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The effect of waxy corn starch (WCS) on the texture, water-holding capacity and microstructure of sardine (Sardina pilchardus) surimi gels in two different systems was studied. In the type A system, increasing amounts of WCS (2, 4, 6 or 8 g/100 g surimi) were added to surimi while maintaining the gel moisture constant at 78%; in the type B system, WCS was added without correcting the gel moisture. Gels were made using two different heat treatments [heat-induced setting (HS) and direct cooking (DC)]. When starch was replaced by surimi (type A) and a heat treatment was applied that favoured formation of a preliminary actomyosin (AM) network (i.e. HS), gel strength (GS) was lower than in the control and decreased as more starch was added, despite an increase in the amount of water held by the gel. Scanning electron microscopy (SEM) showed that the matrix network was fibrillar with a globular surface. Starch appeared to be totally gelatinized and surrounded by a continuous matrix. When the amount of dry matter in gels was increased (type B), in no case did starch have a reinforcing effect, despite an increasing water-holding capacity; SEM showed a denser continuous matrix surrounding the gelatinized starch. Both types of gel made using the heat treatment that allows simultaneous gelling of surimi and gelatinization of starch (i.e. DC) exhibited much poorer GS than did HS gels, while addition of starch made practically no difference to gel texture. The findings suggest that the effect of starch is related to the type of gel matrix that forms upon addition of ingredients. Although such gels contained more water or dry matter, their texture parameters were lower, possibly because of the type of network formed by sardine surimi. Nonetheless, gels of acceptable quality were successfully made with added starch by incorporating less surimi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050048
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  • 2
    Electronic Resource
    Electronic Resource
    The mineralization of mantle dentine and of circumpulpal dentine in the rat: an ultrastructural and element-analytical study (1997)
    Springer
    Anatomy and embryology 195 (1997), S. 289-297 
    Details
    ISSN: 1432-0568
    Keywords: Key words Mineralization ; Dentine ; Ultrastructure ; Elementanalysis ; Collagen fibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The purpose of this study was to compare the biomineralization of circumpulpal dentine with that of mantle dentine by ultrastructural and element-analytical techniques. Forty upper second molar germs of 10-day-old albino rats were cryofixed in liquid nitrogen-cooled propane and embedded in resin after freeze drying. Semithin dry sections were cut for analyzing the calcium and phosphorus concentration in initial mantle dentine, at the mineralization front of circumpulpal dentine, in the middle region of circumpulpal dentine and in mantle dentine peripheral to circumpulpal dentine. For the morphological evaluation of mineral deposits we compared ultrathin and unstained sections of cryofixed molars with chemically fixed molars. For both dentine types it was found that they develop via identical steps of mineral formation at collagen fibrils and non-collagenous matrix molecules. In circumpulpal dentine no globular mineral protrusions along the mineralization front (i.e. calcospherites) and no indications of interglobular dentine at the transition from circumpulpal dentine to mantle dentine were present. The von Korff fibres were not only visible in mantle dentine but also in circumpulpal dentine. Matrix vesicles were present only during the formation of an initial coherent layer of mantle dentine and could not be observed during successive formation of mantle dentine and circumpulpal dentine. The element-analytical data did not demonstrate any difference in the mineral content between the two dentine types. Therefore, we conclude that mantle dentine and circumpulpal dentine in the rat molar possess a high degree of structural and chemical similarity and that only the extent of terminal branching of the odontoblast processes gives an approximate estimation of the thickness of mantle dentine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050048
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  • 3
    Electronic Resource
    Electronic Resource
    Morphological and functional analysis of PTA granules in human NK cells (1997)
    Springer
    Anatomy and embryology 196 (1997), S. 215-226 
    Details
    ISSN: 1432-0568
    Keywords: Key words Natural killer cells ; Ultrastructure ; Parallel tubular arrays ; Perforin ; Granzyme B ; Chondroitin sulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Human natural killer (NK) cells contain unique granules with parallel tubular arrays (PTA granules) of approximately 30 nm diameter that can be seen only by electron microscopy. In order to clarify the role of PTA granules in NK cell-mediated cytolysis we examined these structures with regard to frequency and expression of lytic proteins (perforin, granzymes). NK cells (CD3−, CD16+, CD56+) were obtained from heparinized blood of healthy donors and enriched by double-step negative selection using mAb coupled to magnetic beads. PTA granules were found in 31.3% of freshly separated NK cells. When NK cells were cultivated, even in the presence of various stimulating agents (rhIL-2, rhIL-4, rhIL-6, rhIL-12, GM-CSF, rhIFNα, anti-CD16 mAb, dexamethasone), PTA granules disappeared and transformed into conventional primary lysosomes. By immune electron microscopy using antibodies directed against perforin and granzyme B we observed distinct immuno-reactivity in the tubules and in the tubule-associated faintly electron-dense matrix of PTA granules. Immuno-labelling for perforin and granzyme B was also found in the fine granular matrix of primary lysosomes. Finally, we tested the distribution of chondroitin 4-sulfate which is suggested to inactivate lytic proteins. Immuno-reactivity for chondroitin 4-sulfate was detected only in the moderately electron-dense matrix but not in the tubules of PTA granules. These observations indicate that perforin and granzyme B are stored in an inactive form in PTA tubules due to highly ordered paracrystalline protein folding. As soon as the tubules decay the lytic proteins are kept in an environment of chondroitin 4-sulfate for inactivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050092
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  • 4
    Electronic Resource
    Electronic Resource
    Crural Herbst corpuscles in chicken and quail: numbers and structure (1997)
    Springer
    Anatomy and embryology 196 (1997), S. 323-333 
    Details
    ISSN: 1432-0568
    Keywords: Key words Herbst mechanoreceptors ; Sensory axons ; White Leghorns ; Japanese quail ; Ultrastructure ; Quantitative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Herbst corpuscles were studied in the crural region of perinatal and adult chicken and quail in order to find out their number and dimensions and to learn more about their structure, especially in relation to size. Crural corpuscles are arrayed in an encapsulated string between tibia and fibula. They are closely packed together; a small number of corpuscles is found apart from the string, often attached to the periost. The strings of corpuscles are approximately 40 mm long in adult chicken and 20 mm long in the quail. The crural region of the chicken contains 382.8 ± 90.9 (mean ± SD) corpuscles, the numbers ranging from 301 to 582; in the quail, the mean number is 119.2 ± 27.9, with a range from 83 to 167 corpuscles. In the chicken, one axon supplies an average of 1.60 corpuscles; in the quail, the relation of axons to corpuscles is approximately 0.92. In both species, final numbers of crural corpuscles are already attained before hatching and no difference is found in the mean number and range of corpuscles between perinatal and adult birds. In both chicken and quail, individual strings contain corpuscles of various sizes, from large to very small. The chicken corpuscles are generally twice as large in diameter and often longer than those of the quail. The corpuscles are composed of an axon terminal that projects two rows of axonal spines into the clefts of the inner core and ends with an ultraterminal bulb; the terminal is surrounded with a bilaterally symmetrical inner core, amorphous inner space containing collagen fibrils of various thickness, and a capsule. Large chicken corpuscles contain inner cores composed of up to 100 lamellae, while quail inner cores have half that number at the most. The capsules are usually composed of 8 to 10 lamellar layers in both species, but they are thicker in the chicken than in the quail. The possible functional significance of individual structural components of Herbst corpuscles is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050101
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in motor endplates of the lumbrical muscles of rats induced by a microsomal Ca2+ ATPase inhibitor, 2,5-di(tert-butyl)-1,4-hydroquinone (1997)
    Springer
    Archives of toxicology 72 (1997), S. 115-118 
    Details
    ISSN: 1432-0738
    Keywords: Key words 2 ; 5-di(tert-butyl)-1 ; 4-Hydroquinone ; Ca2+ ATPase inhibitor ; Neurotoxicity ; Ultrastructure ; Motor endplate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Female Wistar rats were treated orally for 5 days with 80 mg/kg body weight of 2,5-di(tert-butyl)-1,4-hydroquinone (DTBHQ), a microsomal Ca2+ ATPase inhibitor. Motor endplates of the lumbrical muscles were examined by light and electron microscopy. There was a decrease in body weight in the treated rats from the first day after administration, and toxic signs appeared after the third day, such as adoption of a prone position, salivation, lacrymation, and an abnormal gait and/or muscle weakness. No remarkable macroscopic or light microscopic changes were noted in the lumbrical muscles as well as other peripheral nerves of hind legs of the treated rats killed 1 day after the last DTBHQ treatment. Ultrastructurally, neurotoxicity characterized by loss of synaptic vesicles and mitochondria in the motor endplates, and by destruction of the motor terminals was detected in the lumbrical muscles of the treated rats. These results strongly indicate that DTBHQ targets the motor endplates in the rat lumbrical muscles and suggest that the resultant damage is responsible for the appearance of neurological signs, such as an abnormal gait and loss of muscle control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002040050477
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural study of human herpesvirus-7 replication in tissue culture (1997)
    Springer
    Virchows Archiv 430 (1997), S. 417-426 
    Details
    ISSN: 1432-2307
    Keywords: Key words HHV-7 ; Viral replication ; Virus morphology ; Ultrastructure ; Herpesviruses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Human herpesvirus 7 (HHV-7) was grown in a CD4+ lymphoblastic cell line (SupT1) and in cord blood mononuclear cells (CBMC). Virus infection was demonstrated by immunohistology with positive control sera, with monoclonal antibodies and by in situ hybridization for viral DNA. Cytopathic effects following HHV-7 infection generally resemble those after HHV-6 infection but are less pronounced. The ultrastructural appearance of HHV-7 and the replicative stages were similar to those described by Kramarsky and Sander for HHV-6. There were some minor discrepancies, including quite an extensive and space-filling tegument, a slightly different structure of the nucleoid, the frequent finding of nucleocapsids without any visible core and apparently scarce or delicate spikes on the envelope. These differences may suggest HHV-7 rather than HHV-6, but this finding needs confirmation. Mature HHV-7 particles measured 170 nm in diameter, with nucleocapsids of 90–95 nm and a tegument of about 30 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050051
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  • 7
    Electronic Resource
    Electronic Resource
    Reinnervation of cat pacinian corpuscles after nerve crush (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 285-293 
    Details
    ISSN: 1432-0533
    Keywords: Key words Pacinian corpuscles ; Reinnervation after ; axotomy ; Regenerated axon terminals ; Ultrastructure ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The reinnervation pattern of crural pacinian corpuscles was examined by light and electron microscopy in eight adult cats of both sexes 3–18 months after sciatic nerve crush. Normal pacinian corpuscles are each supplied with a single myelinated axon and a single cylindrical axon terminal which may branch in the distal part of the inner core. Reinnervation of these vibroreceptors was very satisfactory after sciatic nerve crush: in a sample of 68 corpuscles examined 3–18 months after the operation, 92.6% were found reinnervated, while only 7.4% remained denervated. At the nerve entry, 84.2% of the reinnervated corpuscles were supplied with a single myelinated axon, while 15.8% received two myelinated axons; some of the axons branched before or after entering the inner core. Near the mid-level of the inner core, 60.3% of 63 reinnervated corpuscles were innervated with a single axon terminal, 22.2% were biterminal, while 17.5% had three or more terminals. Regenerated axon terminals induced the formation of thin lamellar layers in the axial region of the original core and, exceptionally, also at the outer aspect of the original core. In monoterminal corpuscles, the shape and ultrastructure of regenerated endings resembled those of normal controls, whereas in multiterminal corpuscles their shape and profiles were variable. In contrast to previous reports, reinnervated corpuscles did not ultimately become monoterminal. On the contrary, the mean number of 1.3 terminals found in reinnervated crural corpuscles at 3–5 months increased to 1.9 terminals per corpuscle 6–18 months after axotomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050616
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  • 8
    Electronic Resource
    Electronic Resource
    Eosinophilic intranuclear inclusions in the hippocampal pyramidal neurons of a patient with amyotrophic lateral sclerosis (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 532-536 
    Details
    ISSN: 1432-0533
    Keywords: Key words Neuronal intranuclear inclusion ; Amyotrophic lateral sclerosis ; Ammon’s horn ; Ultrastructure ; Ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report the presence of round eosinophilic intranuclear inclusions in a patient with sporadic amyotrophic lateral sclerosis (ALS). The inclusions were limited to the hippocampal pyramidal neurons; they were frequently encountered in the CA1 and CA2 regions and much less frequently in the CA3 and CA4 regions and in the subiculum. Ultrastructurally, they consisted of randomly oriented straight filaments, each about 8–14 nm in diameter, some of which had a tubular appearance in cross-section. Electron-dense, granular material was intermingled with the filaments. Immunohistochemically, all the inclusions were positive for ubiquitin, but were negative for several kinds of cytoskeletal protein, including actin, glial fibrillary acidic protein, vimentin, neurofilament polypeptides, keratin, tubulin, tau protein and microtubule-associated protein 2. To our knowledge, this type of neuronal intranuclear inclusion has not so far been reported in ALS, and its distribution limited to the hippocampal formation is of great interest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050649
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  • 9
    Electronic Resource
    Electronic Resource
    Immunocytochemical and ultrastructural study of pericapillary rosettes in amyotrophic lateral sclerosis (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 338-344 
    Details
    ISSN: 1432-0533
    Keywords: Key words Amyotrophic lateral sclerosis ; Pericapillary rosette ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report concerns a comparative immunocytochemical and ultrastructural investigation on pericapillary rosettes (PR) in the lumbar spinal cords of 21 patients with amyotrophic lateral sclerosis (ALS) and 18 age-matched neurologically normal individuals. The purpose of the study was to determine the alteration of PR in relation to the neuronal loss in ALS. The PR were almost always positively immunostained for phosphorylated neurofilament, and some PR immunoreacted with antibodies to synaptophysin and β-amyloid precursor protein. This finding suggests that axonal transport, whether fast or slow, is impaired in the terminal portion of the axon that reaches the capillaries. Some PR were also positively immunostained by the antibody against ubiquitin, anti-calbindin-D 28 K antibody, anti-parvalbumin antibody and the antibody to superoxide dismutase 1. Morphometrically, the number of PR in the anterior horns and lateral column was markedly diminished in ALS compared with controls. At the ultrastructural level, the PR consisted mostly of unmyelinated degenerated axons, and were frequently found outside the basal laminae of the endothelial cell and of the astrocytic foot processes on the opposite side of the capillary, and less often in the space between the two basal laminae. The data indicate that the fate of PR is intimately associated with the neuronal loss of the anterior horn cells and with degenerative change of nerve fibers extending from their mother neurons to the capillaries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050716
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  • 10
    Electronic Resource
    Electronic Resource
    Dynamics of the ultrastructural changes in blood and lymphatic capillaries of bronchi in inflammation and following endobronchial laser therapy (1997)
    Springer
    Virchows Archiv 431 (1997), S. 283-290 
    Details
    ISSN: 1432-2307
    Keywords: Key words Inflammation of the bronchi ; Bronchial biopsy ; Ultrastructure ; Vessels ; Laser therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  An ultrastructural and autoradiographic analysis of changes in 188 biopsy specimens of bronchial mucosa of the large bronchi from 76 patients with chronic inflammatory lung diseses was carried out. Fibrosis results in an apparent reduction of metabolic activity in endothelial cells, affecting the proliferation of basal cells with changes in cell differentiation. Endobronchial laser therapy with an helium-neon laser induces proliferative and metabolic processes in the lamina propria of the bronchial mucosa with hyperaemia, intensive diapedesis of leucocytes and formation of leucocytic infiltrations and granulation tissue. The proliferative and metabolic activity of endothelial and stromal cells increases, and delicate fibrous connective tissue is formed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050100
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  • 11
    Electronic Resource
    Electronic Resource
    Fixation of human detrusor smooth muscle cells: role of osmolarity and magnesium ions on the ultrastructural morphology (1997)
    Springer
    Urological research 25 (1997), S. 283-289 
    Details
    ISSN: 1434-0879
    Keywords: Detrusor ; Fixation ; Magnesium Osmolarity ; Smooth muscle cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Magnesium ions added to fixatives for processing to Transmission Electron Microscopy (TEM) have been claimed to cause relaxation of detrusor smooth muscle cells [1]. This should facilitate the morphologic evaluation of the tissue. However, magnesium ions are osmotically active and their addition may cause the fixative to become hypertonic to the tissue. To ascertain whether the presence of magnesium ions causes significant changes compared to those found where the osmolarity is raised without the presence of magnesium, human detrusor specimens were fixed in glutaraldehyde to which increasing amounts of MgCl2 or NaCl were added in different concentrations. With the addition of increasing amounts of MgCl2 and NaCl, the osmolarity of the fixative increased, causing significant changes in the morphology and morphometry of the tissue. The intercellular distances increased, the cells shrank and the shape of the cells changed from smooth and rounded to spiky and angulated. With regard to its muscle-relaxing effect, it was not possible to distinguish the specimens fixed in magnesium-containing fixatives from those without. In this study it was not possible to prove any relaxing effect of magnesium ions added to the fixative. On the contrary the magnesium ions caused an increase in the osmolarity, with significant changes in both the morphometry and the morphology of the human detrusor smooth muscle cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00942099
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  • 12
    Electronic Resource
    Electronic Resource
    Immunohistochemical and electron microscopical characterization of stromal cells in nasopharyngeal angiofibromas (1997)
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 196-199 
    Details
    ISSN: 1434-4726
    Keywords: Nasopharyngeal angiofibroma ; Ultrastructure ; Myofibroblast ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Twenty-eight cases of nasopharyngeal angiofibroma were studied immunohistochemically for cytoskeletal phenotyping of stromal cells. Electron microscopy was also used to study the ultrastructure of five of the tumors. All typical stromal cells showed intensive immunostaining for vimentin, but were negative for smooth muscle actin and desmin. Ultrastructurally, most of these cells appeared to be exclusively fibroblasts. However, in some areas stromal cells were seen that morphologically resembled myofibroblasts by their shapes and arrangement, and were characterized by the coexpression of vimentin and smooth muscle actin. Electron microscopy confirmed their myofibroblastic nature. The present study showed that the typical stromal cells in nasopharyngeal angiofibromas were fibroblasts and not myofibroblasts. In these tumors myofibroblasts occurred only focally, in connection with fibrotic areas and exclusively as a vimentin+/actin+ cytoskeletal phenotype. This indicates that myofibroblasts are not primary stromal tumor cells in nasopharyngeal angiofibromas, but occur due to regressive changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00879273
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  • 13
    Electronic Resource
    Electronic Resource
    Structural maturation of the interface region between the stria vascularis and spiral ligament in the neonatal rat cochlea (1997)
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 73-77 
    Details
    ISSN: 1434-4726
    Keywords: Development ; Stria vascularis ; Spiral ligament ; Ultrastructure ; Rat cochlea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructural morphology of the interface region between the stria vascularis (SV) and spiral ligament (SL) was examined in the neonatal rat cochlea via transmission electron microscopy. At postnatal day (PND) 3, morphology of both basal cells and fibrocytes was simple and immature. Only a small number of fibrocytes was observed in the SL. Intercellular junctions between basal cells and fibrocytes, and between adjacent fibrocytes, were few. At PND 7, the number of fibrocytes increased, and more organelles appeared within their cytoplasm. From PND 11 to 14, nuclei of the basal cells appeared to be more spindle-shaped and contained more heterochromatin. The cytoplasm of the fibrocytes was pale, and a greater number of cytoplasmic vesicles and mitochondria emerged. More intercellular junctions were observed between basal cells and fibrocytes at the interface region and between fibrocytes in the SL. By PND 21, the morphology of basal cells and fibrocytes and their intercellular junctionsappeared to be adult-like. These morphological observations correlate with previous reports on the functional maturation of the developing rat cochlea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01526182
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  • 14
    Electronic Resource
    Electronic Resource
    Mechanical isolation and ultrastructural characterization of viable egg cells in Plumbago zeylanica (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 368-373 
    Details
    ISSN: 1432-2145
    Keywords: Key words Egg-cell isolation (angiosperm) ; Micromanipulation ; Plumbagozeylanica ; Viable egg ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A protocol for isolating viable eggs in Plumbago zeylanica by mechanical dissection is reported. The optimum solution for isolation was 0.8 M mannitol + 10 mM MOPS + 10 mM CaCl2, (pH 4.5–5.0) with an osmolality of 860–940 mmol/kg. Eggs retain their viability for at least 24 h. Isolated eggs were true protoplasts without cell walls and could tolerate osmolality of 437 mmol/kg to 965 mmol/kg. Observation of the isolated eggs using transmission electron microscopy indicated that they were well preserved and reflected the ultrastructure of physiologically active cells, displaying features similar to those of in vivo egg cells. Notable differences include the absence of a filiform apparatus and the accumulation of dense particles in the plastids, which was most conspicuous in egg cells that were damaged during isolation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050111
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  • 15
    Electronic Resource
    Electronic Resource
    An ultrastructural study of the mature spermatozoid of the fern Asplenium trichomanes L. subsp. trichomanes (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 142-148 
    Details
    ISSN: 1432-2145
    Keywords: Key words Asplenium trichomanes L. subsp. trichomanes ; Ferns ; Spermatozoids ; Flagella ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Asplenium trichomanes L. subsp. trichomanes spermatozoids are spirals of about five turns. Keels link the elements of the microtubular ribbon with the plates of the lamellar layer (LL) which are uninterrupted, parallel and curved with an inner angle of about 150°. Electron-opaque filaments connect the microtubules of the multilayered structure (MLS) and the osmiophilic crest, the LL and the MLS-associated mitochondrion and the latter and the plasmalemma. The nucleus occupies the 2.5–3 posterior turns and has an inner honeycomb-shaped chromatin mass and an outer highly condensed chromatin mass with randomly scattered electron-transparent areas. The basal bodies of the ca. 50 flagella are bounded by a reticulum of granular material which forms a plug inside their proximal region; the proximal region of the flagellum has a 9 + 0 pattern. The axoneme has a 9 + 2 pattern.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050081
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  • 16
    Electronic Resource
    Electronic Resource
    The ultrastructure of skin from a patient with mucopolysaccharidosis IIID (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 210-213 
    Details
    ISSN: 1432-0533
    Keywords: Key words Mucopolysaccharidosis IIID ; Skin ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Mucopolysaccharidosis IIID (MPS-IIID) is the rarest of the MPS-III syndromes. It is caused by deficient activity of lysosomal N-acetylglucosamine-6-sulfatase (G6S). To date, the clinical and biochemical features of seven patients with MPS-IIID have been reported, but no biopsy or autopsy findings have been described. The purpose of this report is to define the ultrastructure of affected cells seen in a skin biopsy from a 14-year-old boy. The child presented with progressive mental deterioration, hyperactivity and mild to moderate dysmorphism. The diagnosis of a mucopolysaccharidosis was suggested, but the initial urine analyses were negative for elevated mucopolysaccharides, and only the third analysis showed abnormal excretion of heparan sulfate. Because of the diagnostic difficulties posed by this case, a skin biopsy was performed for morphological and biochemical studies. Numerous vacuoles were noted in Schwann cells, fibroblasts, smooth muscle cells, eccrine gland and ductal epithelium in resin-embedded sections stained with toluidine blue. Ultrastructurally, many lysosomes were distended with abundant, fibrillar material. Occasionally, lamellated membranous structures were present within the same lysosomes. These findings are consistent with those seen in other forms of MPS, in which the lysosomal storage occurs predominantly, but not exclusively, in mesenchymal cells. Furthermore, deficient activity of G6S was confirmed in cultured skin fibroblasts. This study demonstrates that electron microscopy of skin biopsies is a useful method for identification of patients with clinical features of MPS-IIID whether or not heparan sulfaturia is present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050605
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  • 17
    Electronic Resource
    Electronic Resource
    Ultrastructural concomitants of hypoxia-induced angiogenesis (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 579-584 
    Details
    ISSN: 1432-0533
    Keywords: Key words Hypobaric hypoxia ; Cerebral ; microvasculature ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Prolonged hypoxia results in structural and functional adaptive responses to improve tissue oxygen delivery. Structural changes within the brain include vascular proliferation and elongation. The aim of the current study was to investigate whether ultrastructural changes in capillary walls also occur as part of the adaptive response. Adult rats were exposed to 2 or 3 weeks of moderate hypobaric hypoxia at 0.5 atmospheres and their cerebral microvasculature examined using quantitative ultrastructural methods. We found that hypoxic rats had an 18% increase in their brain capillary diameter but no change in endothelial wall thickness, basement membrane thickness, or coverage of the endothelial wall by pericytes. The increased diameter of cerebral capillaries may play an important role in decreasing the resistance to capillary perfusion which is brought about by the increased erythrocyte fraction in the blood of hypoxic rats. Ultrastructural features relevant to the blood-brain barrier were maintained in hypoxic rats. Pericytes, that are thought to form a second line of defense in the blood-brain barrier, maintained their numerical and size relationships to the endothelial cells. Endothelial junctions were unchanged and endothelial vesicles were somewhat lower in density than normal at 2 weeks of hypoxia, but had regained their normal density by 3 weeks. Mitochondria of the brain capillary endothelial cells maintained normal numerical and volume densities in hypoxia, but the mitochondria of the surrounding neuropil were decreased significantly by about 30%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050654
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  • 18
    Electronic Resource
    Electronic Resource
    Central neurocytoma: an immunohistochemical, ultrastructural and cell culture study (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 425-435 
    Details
    ISSN: 1432-0533
    Keywords: Key words Cell culture ; Central neurocytoma ; Histogenesis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To clarify the histogenesis and differentiation potential of central neurocytoma, a pathological investigation of seven tumors from three patients was conducted using immunohistochemistry and ultrastructural analysis in addition to systematic in vitro studies. Six tumors were studied immunohistochemically and five were examined ultrastructurally. All cases that were immunostained were positive for synaptophysin in nuclear-free neuropil islands. In five tumors, a few tumor cells, in addition to reactive astrocytes, were positive for glial fibrillary acidic protein (GFAP). Vimentin staining was also positive in a few tumor cells of five specimens. Neurofilament staining was always negative. All cases for which ultrastructure was examined showed various synaptic abnormalities. Cultured cells were subdivided into three distinct tumor cell types: neuronal cells which stained for neurofilament proteins with neurosecretory granules; small flat undifferentiated cells with a high nuclear-cytoplasmic ratio and scant cytoplasmic organelles; and small round or multipolar astrocytic cells with 10-nm intermediate filaments which stained for GFAP. Our tissue culture studies disclosed that cultured neurocytoma cells form a cellular mosaic similar to subependymal plate layers that are composed of mitotically active cells, neurons and glia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050729
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  • 19
    Electronic Resource
    Electronic Resource
    Intracytoplasmic chromophobe inclusion bodies in an anaplastic meningioma (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 421-425 
    Details
    ISSN: 1432-0533
    Keywords: Key words Meningioma ; Inclusion body ; Vimentin ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracytoplasmic inclusion bodies are rarely found in meningiomas. A 74-year-old woman had an anaplastic meningioma with intracytoplasmic chromophobe inclusion bodies (CIB) histologically. These CIB were various shapes, e.g. round, teardrop-like, fusiform, horseshoe-like, crescentic and perinuclear. The size of CIB ranged from 7 to 14 μm and the nuclei of the tumor cells with CIB were often eccentric. Most CIB were immunopositive only for vimentin, staining more intensely than surrounding cytoplasm in a comparative study using adjacent sections stained with hematoxylin-eosin and vimentin. CIB showed loosely textured filamentous structures which were in parallel and entangled arrangements ultrastructurally. The diameter of the filaments was 13–14 nm and they were thicker than normal intermediate filaments. Moreover, these filaments appeared to be studded with granular and fuzzy substances. These findings suggest that CIB are mainly composed of abnormally synthesized and arranged vimentin filaments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050634
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  • 20
    Electronic Resource
    Electronic Resource
    Characterization of a prolonged regenerative attempt by diffusely injured axons following traumatic brain injury in adult cat: a light and electron microscopic immunocytochemical study (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 329-337 
    Details
    ISSN: 1432-0533
    Keywords: Key words Regeneration ; Diffuse axonal injury ; GAP43 ; Neurofilament ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Traumatic brain injury in animals and humans is well known to cause axonal damage diffusely scattered throughout the brain without evidence of other brain parenchymal change. This observation has prompted some to posit that such damaged axons are well positioned to mount a regenerative attempt. The present study uses an immunocytochemical marker specific for regenerating neurites to explore this issue. Further, in an attempt to expedite and enhance any potential regenerative effort, this study evaluates the efficacy of intrathecally applied nerve growth factor. Three sets of experiments were performed in adult cats. One group of animals was subjected to moderate fluid percussion brain injury and followed for 7 or 14 days post injury, with the continuous intraventricular infusion of nerve growth factor delivered by implanted osmotic pumps. These animals were compared to a second group of time-matched, sham-operated animals receiving artificial cerebrospinal fluid infusion. To assess axonal damage immunohistochemical staining for the low molecular weight neurofilament subunit (NF-L) was carried out using an NR4 monoclonal antibody. To localize axons exhibiting a regenerative response immunohistochemical staining for the growth associated protein GAP43 was employed. In sham controls, at the light microscopic level NF-L-immunoreactive axonal swellings were numerous at 7 days, but by 14 days post injury their frequency declined markedly. In contrast, GAP43-immunoreactive, disconnected reactive axonal swellings were rarely observed at 7 days but were numerous at 14 days. Ultrastructural analysis at 14 days post injury of carefully matched sections revealed reactive axons demonstrating sprouting consistent with a regenerative effort. Analysis of tissue from animals of 14 days of survival indicated that supplementation with nerve growth factor did not appear to enhance the capacity of damaged brain axons to mount a regenerative attempt. Rather, it appears that regenerative efforts seen reflect a spontaneous response. A third group of adult cats, subjected to the same injury but not subjected to osmotic pump implantation, was allowed to survive for 22–28 days. Animals in this group also demonstrated GAP43 immunoreactivity in reactive axonal swellings in the brain stem. This study demonstrates that diffusely injured axons can mount a sustained regenerative attempt that is associated with a reorganization of their cytoskeleton and accompanied by an up-regulation of growth-associated proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050715
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  • 21
    Electronic Resource
    Electronic Resource
    An unusual meningioma variant with glial fibrillary acidic protein expression (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 499-503 
    Details
    ISSN: 1432-0533
    Keywords: Key words Meningioma ; Immunohistochemistry ; Glial fibrillary acidic protein ; Ultrastructure ; Intercellular lumina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We studied a recurrent meningioma located in the right frontal lobe. The tumor showed high cellularity and the cells had plump, hyalinous cytoplasm. Immunohistochemically, almost all the tumor cells were positive for epithelial membrane antigen and vimentin, and unexpectedly, glial fibrillary acidic protein (GFAP). Ultrastructural investigation revealed abundant 8- to 10-nm filaments in the cytoplasm. Conspicuous interdigitations with numerous desmosomes were present. Frequently, intracellular and intercellular lumina lined by microvilli were also found. We considered the present case to be an unusual variant of meningioma with GFAP expression. A few cases of meningioma with triple expression of GFAP, vimentin and cytokeratin have been reported previously. However, the present case showed obvious pathological differences from these, and had no immunoreactivity for cytokeratin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050739
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  • 22
    Electronic Resource
    Electronic Resource
    Electron microscopy and morphometry enhances differentiation of epidermolysis bullosa subtypes. With normal values for 24 parameters in skin (1997)
    Springer
    Archives of dermatological research 289 (1997), S. 631-639 
    Details
    ISSN: 1432-069X
    Keywords: Key words Diagnosis ; Mechanobullous disease ; Skin ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Electron microscopy combined with morphometry was used to establish values for 24 parameters in normal skin. These results were compared with those similarly obtained from samples of epidermolysis bullosa with a view to facilitating classification of the disease. Six of the eight subtypes of epidermolysis bullosa investigated could be differentiated. Four subtypes showed values for structural components in intact skin which were outside the normal range: (1) epidermolysis bullosa simplex generalisata gravis (hemidesmosomes); (2) epidermolysis bullosa dystrophica Pasini and (3) Cockayne-Touraine (anchoring fibrils); and (4) epidermolysis bullosa acquisita (anchoring fibrils, hemidesmosomes, and lamina lucida and lamina densa aspects of the dermoepidermal junction). Two subtypes revealed specific features which could be assessed qualitatively: distinctive, circumscribed, clumped tonofilament bodies were present in basal keratinocytes from epidermolysis bullosa herpetiformis Dowling-Meara and thick (30 nm diameter) cross-striated anchoring fibrils were absent in epidermolysis bullosa dystrophica generalisata gravis. Epidermolysis bullosa simplex Köbner and Weber-Cockayne forms could not be distinguished.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050252
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  • 23
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in rat locus coeruleus induced by chronic opioids (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 109-115 
    Details
    ISSN: 1432-0533
    Keywords: Key words Locus coeruleus ; Ultrastructure ; Dihydroetorphine ; Morphine ; Opioids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The locus coeruleus (LC) is a major noradrenergic nucleus in the brain. The activity of the LC neurons is chronically regulated by opioids. So far, very little is known about the morphological changes induced by chronic treatment with opioids. In the present study, the effects of chronic treatment with morphine and dihydroetorphine, a new narcotic analgesic with lower physical dependence potential than morphine, were investigated on the ultrastructure of the rat LC. Rats received saline or increasing doses of morphine or dihydroetorphine for 5 days by twice daily subcutaneous injections. Withdrawal was precipitated in half of the opioid-treated rats by a single intraperitoneal injection of naloxone 4 h after the last injections of opioids. The ultrastructure of the LC was examined by electron microscopy. Results showed that chronic morphine treatment induced a marked injury to the LC neurons. The primary changes in the cell body were the indentation of nuclei, the fragmentation and degranulation of rough endoplasmic reticulum, as well as the disaggregation of polyribosomes. Myelinoid bodies were seen in the processes. An accumulation of presynaptic vesicles was observed in some of the terminals which formed synaptic junctions with the LC neurons as compared to the normal controls. Naloxone-precipitated withdrawal from morphine did not stop the morphine-induced injury on the LC neurons except that less accumulation of presynaptic vesicles occurred. Chronic dihydroetorphine treatment only induced a slight change in the ultrastructure of the LC neurons. These results indicate that the LC neurons are more vulnerable to chronic treatment with morphine than to that with dihydroetorphine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050681
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  • 24
    Electronic Resource
    Electronic Resource
    Spatial distribution of β-spectrin in normal and dystrophic human skeletal muscle (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 240-246 
    Details
    ISSN: 1432-0533
    Keywords: Keywords Spectrin ; Dystrophin ; Ultrastructure ; Duchenne muscular dystrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Spectrin, a major component of the erythrocyte membrane skeleton, has previously been shown to form a two-dimensional lattice in erythrocytes, and in avian or chicken skeletal muscle. Those results were mainly obtained with antibodies against α-spectrin. Using immunofluorescence of semithin cryosections and single muscle fiber preparations, we show here that β-spectrin forms a costameric network which covers the plasma membrane of human skeletal muscle. These spectrin costameres are correlated with the Z-bands. They are longitudinally connected by fine strands and interrupted by myonuclear lacunae. Under mechanical stretching, the costameres retained their correlation to the Z-bands in normal and dystrophin-deficient muscle, up to the point at which the sarcolemma was disrupted. In stretched muscle, in some regions of the stretched fibers in which the costameres seemed to form double strands, the usually 1:1 correlation of spectrin to the Z-bands changed to a 2:1 relation. In dystrophin-deficient muscle, the costameric scaffold of spectrin in the well-preserved fibers appeared normal, indicating that spectrin can be correctly localized in the absence of dystrophin and that the subcellular spectrin organization does not primarily depend on dystrophin expression. The regular organization and the correlation of spectrin costameres to the Z-bands was notable even in stretched Duchenne muscular dystrophy (DMD) muscle. On the other hand, single teased muscle fibers of DMD muscle showed various degrees of morphological alterations of the costameric network, ranging from a focal disarray to complete loss of costameric organization. Because these findings indicate that the costameric spectrin scaffold undergoes secondary changes during the course of the dystrophic process in dystrophin-deficient muscle, spectrin staining of isolated muscle fibers may also serve as a tool to monitor the effect of gene therapy experiments at the single fiber level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050699
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  • 25
    Electronic Resource
    Electronic Resource
    Ultrastructural study of the proboscis endothelium of Riseriellus occultus (Nemertea, Heteronemertea) (1997)
    Springer
    Hydrobiologia 365 (1997), S. 121-127 
    Details
    ISSN: 1573-5117
    Keywords: Riseriellus occultus ; Heteronemertea ; Proboscis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined with transmission electron microscopythe epithelial layer exposed to the rhynchocoel fluidof the proboscis in the heteronemertine Riseriellus occultus. This epithelium is organized asa monociliated, pseudostratified myoepitheliumconsisting of two cell types: apically situatedmonociliated supportive cells and subapical myocyteslacking cilia. The low supportive cells form acontinuous adluminal sheet and reach with numerouscytoplasmic processes into the extracellular matrix;these cells are characterized by numerous, irregularlyshaped, apical folds projecting into the rhynchocoelfluid, delimiting broad extracellular spaces. Theauthors suppose that both apical and basal folds couldaccommodate stretching of the endothelium when theproboscis is everted. The apical folds of thesupportive cells increase the interface of these withthe rhynchocoel fluid; this feature, together with thepresence of pinocytotic vesicles in such cells,suggest that they could be involved in the exchange ofsubstances between the rhynchocoel fluid and theproboscis. The myocytes are scattered singly withinthe monociliated pseudostratified myoepithelium. Theyare situated between the supportive cells and thesubjacent extracellular matrix. Basement membraneseparating both cells types is lacking. Myofibrillarparts protrude basally from the myocyte somata. Themyofibrillar parts lie in direct apposition to theextracellular matrix, and are oriented circular to thelongitudinal axis of the proboscis. We consider themyocytes to be intra-epithelial, myoepithelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003101703985
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  • 26
    Electronic Resource
    Electronic Resource
    Mucosal surface area in chicken small intestine during development (1997)
    Springer
    Cell & tissue research 290 (1997), S. 71-78 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Development ; Mucosal surface area ; Ultrastructure ; Villus ; Microvillus ; Morphometric analysis ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The mucosal surface area of the chicken duodenum, jejunum, and ileum was determined during development (from 1-day to 12-week-old animals). The morphometric analysis was performed at three magnification levels. The nominal (serosal) surface area was determined at the macroscopic level, from intestinal length and perimeter. Villus and microvillus amplification factors were estimated at light-microscopic and transmission electron-microscopic levels, respectively. The results show, during the period considered: (1) a similar increase in nominal surface area for the three segments (6.5 to 7.2-fold), (2) a rise followed by a slight decrease in the villus amplification factor in the third week of age in the duodenum, a two-fold increase of this variable in the jejunum and no significant developmental variations in the ileum, (3) an increase in the microvillus amplification factor of 1.5-fold in the duodenum and jejunum and of 1.2-fold in the ileum, although a pronounced decrease in the first week of age was observed in the three segments. In conclusion, total mucosal surface area increased, from 1 day to 12 week, 12- to 13-fold in the duodenum and ileum and 20-fold in the jejunum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050909
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  • 27
    Electronic Resource
    Electronic Resource
    The peroxisomes of the hepatopancreas in two species of chitons (1997)
    Springer
    Cell & tissue research 290 (1997), S. 655-664 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Peroxisomes ; Ultrastructure ; Digestive gland ; Acanthochiton crinita ; Lepidochitona cinerea (Mollusca ; Polyplacophora)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract . This paper presents the first description of peroxisomes in polyplacophorans. As in other molluscs, the hepatopancreas of chitons is composed of basophilic and digestive cells. In the basophilic cells, the endoplasmic reticulum is abundant and several Golgi stacks can be observed. These cells also possess secretion granules and vacuoles with spherites. The digestive cells are mainly characterized by the presence of many food vacuoles. Several peroxisomes were observed in the basophilic cells of Acanthochiton crinita, most of them almost spherical. The matrix is filled with tubular structures and a crystalline nucleoid is also present in these organelles. In the digestive cells of A. crinita, peroxisomes are also almost spherical and possess two kinds of nucleoids. One of them presents a diamond shape and a bundle of tubular structures forms a second kind of nucleoid, which shows an elongated form. In Lepidochitona cinerea, the peroxisomes of basophilic cells are spherical or oval. Within the matrix, a cluster of dense rods and a prismatic nucleoid were observed. In the digestive cells of this species, almost spherical or oval peroxisomes are common, but they are smaller than the peroxisomes of the preceding cells. Nucleoids were not detected, but a few dense rods could be observed in the matrix. In both cell types of the two species, catalase activity was detected in the peroxisomal matrix. In addition, the elongated nucleoid of A. crinita digestive-cell peroxisomes and the nucleoid of L. cinerea basophilic-cell peroxisomes also present catalase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050971
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  • 28
    Electronic Resource
    Electronic Resource
    Reversibility of omeprazole-evoked changes in the ultrastructure of ECL cells in the rat stomach (1997)
    Springer
    Cell & tissue research 291 (1997), S. 91-95 
    Details
    ISSN: 1432-0878
    Keywords: Key words ECL cells ; Omeprazole ; Granules/vesicles ; Ultrastructure ; Stomach ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The ECL cells are histamine- and peptide hormone-producing endocrine cells in the rat oxyntic mucosa. They are rich in secretory vesicles and also contain microvesicles and electron-dense granules. They operate under the control of circulating gastrin. In the present study, we examined the ECL-cell ultrastructure after long term treatment with omeprazole, which is known to induce hypergastrinemia, and after withdrawal of the drug. Rats received omeprazole (400 µmol/kg per day, orally) for 16 days and were killed 1, 5, 20, or 40 days after the last dose of the drug. Oxyntic mucosal specimens were processed for electron microscopy. Electron micrographs of ECL-cell profiles were analyzed planimetrically. The ECL-cell profile area increased promptly in response to omeprazole, the secretory vesicles and granules were reduced in number and volume density, the microvesicles were unchanged in number but reduced in volume density, and vacuoles appeared. Within a week after stopping the omeprazole treatment, the numbers and volume densities of secretory vesicles and microvesicles returned to pre-stimulation values. Also, the vacuoles disappeared promptly. The ECL-cell profile area decreased below the pre-stimulation level within five days after stopping treatment, while, in contrast, the granules increased in number and volume density. Somewhat surprisingly, the cell size and the granule compartment did not return to normal until 40 days after stopping treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050982
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  • 29
    Electronic Resource
    Electronic Resource
    Development of striated rootlets during ciliogenesis in the human oviduct epithelium (1997)
    Springer
    Cell & tissue research 290 (1997), S. 39-42 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Ciliogenesis ; Striated rootlets ; Oviduct ; Ciliated cells ; Ultrastructure ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the same time as ciliary budding. After the formation of several striations, the tip of the rootlets extended deeply toward the interior of the cell and became differentiated into two distinct parts, viz., the proximal conical part connected to the basal body and the distal fibrillar part. The periodicity of the striations in the fibrillar part was 68.5±2.95 nm, about 5 nm longer than that of the conical part (63.9±2.25 nm). The dark band in the striation was thicker in the fibrillar part than in the conical part. Since the fibrillar part was not observed in the mature cilium, this part was considered as being either degraded or changed into the conical part during ciliogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050905
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  • 30
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of basic proteins ofBlastocystis hominis (1997)
    Springer
    Protoplasma 200 (1997), S. 31-34 
    Details
    ISSN: 1615-6102
    Keywords: Blastocystis hominis ; Central vacuole ; Accumulation ; Basic proteins ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Basic proteins ofBlastocystis hominis were detected by the ammoniacal silver and ethanolic phosphotungstic acid techniques using electron microscopy. The central vacuole showed many silver grains when treated with ammoniacal silver and an increased electron density when treated with phosphotungstic acid. The intensity of positive reactions correlated with the electron density of the central vacuole, because cells having an electron-lucent central vacuole showed no silver grain deposits. Since it is known that the concentration of electron-dense materials in the central vacuole increases during log phase of growth, and then decreases in stationary phase, this organelle must accumulate basic proteins during cell growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280732
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    Electronic Resource
    Electronic Resource
    Ultrastructural characterisation of cells specialised for nitrogen fixation in a non-heterocystous cyanobacterium,Trichodesmium spp. (1997)
    Springer
    Protoplasma 197 (1997), S. 76-85 
    Details
    ISSN: 1615-6102
    Keywords: Cell differentiation ; Immunolocalisation ; Nitrogenase ; Non-heterocystous cyanobacteria ; Trichodesmium ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Trichodesmium is the first described example of a filamentous cyanobacterium without heterocysts that contains cells specialised for nitrogen fixation. The ultrastructure of cells with and without nitrogenase were compared using primarilyTrichodesmium tenue Wille, but alsoT. thiebautii Gomont andT. erythraeum Ehrenberg et Gomont. Immunohistochemistry demonstrated that the cytoplasm of certain cells was densely labelled with antibodies against Fe-protein (dinitrogenase reductase). Comparative TEM-image analysis revealed that these cells were also distinguished by a denser thylakoid network, dividing the vacuole-like space into smaller units. The nitrogenase-containing cells also exhibited less extensive gas vacuoles as well as fewer and smaller cyanophycin granules compared to cells which lacked nitrogenase. Carboxysomes were present in both cell types in equal proportion. Longitudinal sections showed that cells with nitrogenase were arranged adjacent to each other, and that groups of cells with and without nitrogenase may coexist in the same trichome. The correlation between modifications in ultrastructure and the presence of nitrogenase suggests a new type of cyanobacterial cell specialisation related to nitrogen fixation. The results obtained also question the systematic affiliation of the genusTrichodesmium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279886
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    Electronic Resource
    Electronic Resource
    Apical tubular network in the rat kidney proximal tubule cells studied by thick-section and scanning electron microscopy (1997)
    Springer
    Cell & tissue research 288 (1997), S. 317-325 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Kidney (proximal tubule) ; Apical tubule ; Endosome ; Ultrastructure ; Endocytosis ; Membrane recycling ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The apical cytoplasm of several absorbing epithelia contains well-developed apical tubules (AT) which contribute to membrane recycling from endocytic vacuoles to the apical cell membrane. In this study, we examined three-dimensional structures of the AT in rat kidney proximal tubule cells by transmission and scanning electron microscopy. In thin sections, the AT appeared as straight tubules with a rather constant diameter (70–90 nm), but others were curved and, occasionally, branching. No AT were labeled with the marker for the external cell surface (ruthenium red) or exhibited histochemical enzyme activity for lysosomal hydrolase (acid phosphatase). After intravenous injection of horseradish peroxidase, it was absorbed in the kidney proximal tubule cells and the AT were labeled with HRP reaction products. Stereo-viewing of the labeled AT in thick sections revealed that they formed an interconnected tubular network. Scanning electron microscopy allowed a three-dimensional view of the AT, in which a network of branching and anastomosing tubules was revealed. These observations indicate that the AT are intracellular endosomal compartments which form an extensive tubular network in the apical cytoplasm. The possibility that this apical tubular network serves as a large membrane store for membrane recycling is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050817
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  • 33
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    Electronic Resource
    Exocytosis in the antral gastrin cells of mouse, rat, and guinea pig after stimulation by carbamylcholine (1997)
    Springer
    Cell & tissue research 289 (1997), S. 463-472 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Exocytosis ; Endocytosis ; Gastrin cells ; Carbamylcholine ; Ultrastructure ; Pyloric antrum ; Guinea pig (Hartley) ; Mouse (ICR) ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In order to capture the exocytotic figures of gastrin cells in the pyloric antrum of the stomach, we examined antral cells of the mouse, rat, and guinea pig by electron microscopy following stimulation with the cholinergic secretagogue carbamylcholine. Increased numbers of omega profiles indicative of exocytosis were seen in the basal or lateral cell membrane after stimulation with carbamylcholine. The number of exocytotic figures in stimulated gastrin cells was higher in the guinea pig than in the mouse and rat. Coated and non-coated omega profiles and coated pits in the plasma membrane were smaller than the secretory granules. Omega profiles with or without electron-dense contents were seen. Coated and non-coated vesicles were often visible near the plasma membrane of stimulated gastrin cells in all three species, large cytoplasmic vacuoles also being found in the guinea pig. In the mouse pretreated with horseradish peroxidase, reaction deposits were observed in the omega profiles and in microvesicles near the plasma membrane. These results suggest that, after exocytosis, membrane retrieval and endocytosis occur in the gastrin cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050892
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  • 34
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    Electronic Resource
    Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is localized over the entire plasma membrane of endothelial cells (1997)
    Springer
    Cell & tissue research 290 (1997), S. 623-631 
    Details
    ISSN: 1432-0878
    Keywords: Key words: PECAM-1 (platelet/endothelial cell adhesion molecule-1) ; Endothelium ; HUVEC (human umbilical vein endothelial cells) ; Myocardium ; Ultrastructure ; Human ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The subcellular localization of PECAM-1 in endothelial cells was examined by using advanced morphological techniques, such as confocal scanning microscopy and immunolabeling procedures for electron microscopy. The localization of PECAM-1 was studied immunohistochemically with five specific monoclonal antibodies and one polyclonal antibody (all anti-human) in human and rabbit myocardium and in isolated endothelial cells. In vivo, PECAM-1 was localized uniformly on the plasma membrane of all vascular endothelial cells, predominantly on the luminal side of vessels. No specific increase in labeling was found at sites of cell-to-cell contact. In vitro, primary isolated cells (human umbilical vein endothelial cells) showed continuous labeling of the entire cell membrane. Cells of higher passages were labeled in a manner similar to freshly isolated cells. Our findings refute the commonly accepted hypothesis that PECAM-1 is localized only at cell-to-cell contacts. Further, we have not been able to confirm the hypothesis regarding the important mechanical role of PECAM-1 in stabilizing the endothelial monolayer. Since PECAM-1 is also expressed on platelets and is known to bind to itself, the way in which PECAM-1-positive endothelial cells are protected against binding of PECAM-1-positive platelets remains unclear. In view of these findings, the role of PECAM-1 in the leukocyte migration cascade needs to be re-evaluated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050968
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  • 35
    Electronic Resource
    Electronic Resource
    Cardiomyopathy secondary to systemic triglyceride storage disease (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 88-91 
    Details
    ISSN: 1860-1499
    Keywords: Systemic triglyceride storage disease ; Cardiomyopathy ; Endomyocardial biopsy ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ultrastructural changes of a biopsied myocardium were observed by transmission electron microscopy in a patient with cardiomyopathy secondary to systemic triglyceride storage disease with Jordans' anomaly. There were many lipid droplets in the cardiocytes, and lipofuscin and mitochondria were increased. The volume fraction of myofibrils in the cardiocytes decreased because of an abundance of lipid droplets and mitochondriosis. Myocardial contractility may have been reduced by myofibrillar scarcity and low energy production resulting from an abnormality in the metabolism of fatty acids in the cardiocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545087
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  • 36
    Electronic Resource
    Electronic Resource
    Ultrastructural and biochemical studies of ischemic preconditioning using an adenosine receptor blocker and an ATP-sensitive K channel opener (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 63-69 
    Details
    ISSN: 1860-1499
    Keywords: Preconditioning ; Ultrastructure ; Ischemia and reperfusion ; Adenosine receptor ; Nicorandil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of preconditioning (PC) on acute ischemic myocardial injury was investigated in an openchest dog model. Preconditioned dogs received four 5-min occlusions of the left anterior descending coronary artery (LAD), each separated by 10 min of reperfusion. Four groups were used to assess the effect: non-PC group (G-1), PC group (G-2), 8-phenyltheophylline-(adenosine receptor blocker) infused PC group (G-3), and nicorandil- (ATP-sensitive K-channel opener) infused PC group (G-4). The LAD was occluded for 60 min, followed by 60 min of reperfusion in all dogs. The rate of ultrastructural myocardial severe injury was 26% in G-1, 0% in G-2, 5% in G-3, and 0% in G-4. Biochemical analayses also indicated higher values of myocardial contractile function in G-2 and G-4 than G-1 and G-3. These data suggest that the adenosine receptor and K channel may play a key role in PC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545083
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  • 37
    Electronic Resource
    Electronic Resource
    An ultrastructural study of the influence of radiation against cell line-derived human oral malignant melanoma (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 81-87 
    Details
    ISSN: 1860-1499
    Keywords: Oral malignant melanoma ; Ultrastructure ; Radiosensitivity ; Nucleus ; Melanosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The response to radiation of the SSM-1 cell line, derived from oral malignant melanoma, was investigated by survival curve and by light and electron microscopic abservation. Survival curve of the cells was investigated by the colony formation assay. X-irradiation with 2, 4, and 8 Gy was performed against the cell line. Morphological changes of the cells at 6, 24, 72, and 120h after irradiation were examined by both light and electron microscopy. The survival curve of the SSM-1 cells showed higher radiosensitivity than that of cutaneous melanoma cell lines. Giant cells and multinucleated cells were found only 120h after 8Gy irradiation. Ultrastructural observation revealed changes in nucleus and melanosomes; melanosomes increased in number at 120h after 8Gy irradiation. Further alterations after irradiation were noticed primarily in the nucleus. Radiosensitivity of the SSM-1 cell line derived from oral malignant melanoma was higher than that of cutaneous melanoma cell lines. The results of this study may support the concept that radiotherapy is effective for oral malignant melanoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545086
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  • 38
    Electronic Resource
    Electronic Resource
    Effects of short-term treatment with ethanol on the ultrastructure of the adult golden hamster parathyroid gland (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 148-153 
    Details
    ISSN: 1860-1499
    Keywords: Parathyroid gland ; Ultrastructure ; Golden hamster ; Ethanol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Several previous studies have indicated that ingestion of ethanol can induce hypocalcemia or osteoporosis. However, few data are available concerning the effects of ethanol on the parathyroid gland. To clarify the mechanism of ethanol-induced hypocalcemia, we studied the ultrastructure to the parathyroid gland in golden hamsters after shortterm treatment with ethanol. Ethanol was administered by gavage via an intragastric tube at 6g/kg of 50% ethanol in distilled water. The mean serum calcium concentration was significantly low at 3 and 5h after administration. The Golgi complexes of the parathyroid chief cells significantly decreased 1 and 3h after administration. The lipid droplets and the large vacuolar bodies significantly increased 5h after administration. These findings suggest that the cellular activity of the parathyroid gland is suppressed after shortterm treatment with ethanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545316
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  • 39
    Electronic Resource
    Electronic Resource
    Ultrastructural changes and localization of nitri oxide synthase in rat lung induced by endotoxin administration (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 177-184 
    Details
    ISSN: 1860-1499
    Keywords: Nitric oxide synthase (NOS) ; Endotoxin ; Lung ; Ultrastructure ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the relationship between pulmonary damage and the induction of nitric oxide synthase (NOS) in endotoxin shock, we injected 10mg/kg ofE. coli endotoxin intraperitoneally to Wistar male rats and observed the changes of the lung during the following 8h by electron microscopy, immunoelectron microscopy, and in situ hybridization. Morphological observation revealed infiltration of macrophages, aggregation of neutrophil in stasis in vascular lumens, and intraalveolar hemorrhage accompanied by epithelial damage. Endothelial constitutive NOS (ecNOS) was immunohistochemically localized in the endoplasmic reticulum of the endothelium of pulmonary arteries and in the cytoplasm of bronchial epithelial cells of control rats. After endotoxin administration, inducible NOS (iNOS) was detected in vascular endothelial cells, vascular smooth muscle cells, bronchial epithelial cells, bronchial smooth muscle cells, alveolar epithelial cells, and macrophages. Reverse transcription polymerase cham reaction (RTPCR) confirmed the expression of ecNOS mRNA and iNOS mRNA in the lung in endotoxin-treated rats and controls. In situ hybridization showed that ecNOS mRNA was expressed in vascular endothelial cells of pulmonary arteries in control rats. After endotoxin administration, iNOS mRNA was expressed in vascular endothelial cells. vascular smooth muscle cells, bronchial epithelial cells, alveolar epithelial cells, and macrophages that had infiltrated the alveolar and perivascular regions. After endotoxin administration, morphological changes and NO overproduction were observed, and it is concluded that NO may play an important role in maintaining the homeostasis of the bloodair barrier in pulmonary structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545768
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  • 40
    Electronic Resource
    Electronic Resource
    Physiological and structural changes in the chloroplast of the green alga Micrasterias denticulata induced by UV-B simulation (1997)
    Springer
    Plant ecology 128 (1997), S. 55-64 
    Details
    ISSN: 1573-5052
    Keywords: Algae ; Chloroplast ; Micrasterias ; Photosynthesis ; Ultrastructure ; UV-B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exposure of postmitotic growing and non-growing cells of the unicellular green alga Micrasterias denticulata to different UV-B cut-off wavelengths together with simulated sunlight in a sun simulator has revealed a marked resistence of the algae against strong irradiation. While down to a cut-off wavelength of 284 nm irradiated during the most sensitive stage of cell development chloroplast ultrastructure remains unaffected, severe changes in arrangement and structure of stroma and grana thylakoids occur only at the lowest cut-off wavelengths of 280 and 275 nm. The structural alterations end up in a more or less complete desintegration of grana and stroma thylakoids with the remaining membraneous structures appearing in negative staining thus indicating drastic changes in membrane composition. Photosynthetic activity determined by chlorophyll fluorescence (ratio of variable to maximal fluorescence) and oxygen evolution responded more sensitively to UV-B irradiation. With decreasing UV cut-off wavelengths and prolonged incubation a decrease of photochemistry of PS II occured reaching its lowest values after 60 min at 275 and 280 nm. Oxygen production was even maintained under strong UV irradiation with a cut-off wavelenght of 275 nm up to 15 min. With prolonged UV-B treatment any activity was lost. HPLC separations of pigments exhibited the appearance of break-down products (mainly derivatives of chl b and chl a) with decreasing cut-off wavelength and increasing exposure time. The xanthophyll cycle pigments seemed to be unaffected at least for an irradiation period of 60 to 90 min at low UV cut-offs. Possible mechanisms of UV stress avoidance or protection are discussed with regard to the varying altitudes of the natural habitats of the algae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009754722357
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  • 41
    Electronic Resource
    Electronic Resource
    Squamous cell characteristics in small cell carcinoma of the uterine cervix: histological, immunohistochemical, and ultrastructural study in xenografted small cell carcinoma (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 202-208 
    Details
    ISSN: 1860-1499
    Keywords: Uterine cervix ; Small cell carcinoma ; Xenograft ; Immunohistochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Small cell carcinoma of the uterine cervix is a rare type of gynecological tumor that frequently expresses neuroendocrine differentiation. Its histological origin is unclear. We examined the histopathological characteristics of small cell cervical carcinoma in a patient with elevated serum adrenocorticotropin hormone. We then studied the morphological alteration in xenotransplanted tumors (passages 1–9) using immunohistochemistry and electron microscopy. The primary cervical tumor was characterized by a sheetlike arrangement of uniform small cells with hyperchromatic nuclei and a high nucleocytoplasmic ratio. A ribbon-like or trabecular pattern was also observed in a small area of the tumor. Neuron-specific enolase, chromogranin A, and S-100 were positive for the tumor cells, but cytokeratin was negative. Dense-core granules were detected by electron microscopy. In the xenografted tumor, a serial change from squamous cells to round-to-oval cells was observed. Cytokeratin was immunohistochemically stained in the squamous tumor cells but not in the other tumor cells. In contrast, chromogranin A was stained in some of the round-to-oval cells. Basal lamina underlaid the squamous tumor cells, and desmosome-like junctions were apparent. The cytoplasm was filled with well-differentiated organelles including electron-dense tonofilaments. Elliptical tumor cells resembled the primary carcinoma ultrastructurally. These findings suggest that small cell cervical carcinoma with neuroendocrine properties shares the characteristics of squamous cell carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545771
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  • 42
    Electronic Resource
    Electronic Resource
    Neuronal differentiation of human neuroblastoma SH-SY5Y cells by gangliosides (1997)
    Springer
    Brain tumor pathology 14 (1997), S. 5-11 
    Details
    ISSN: 1861-387X
    Keywords: Gangliosides ; Neuronal differentiation ; Neuroblastoma ; Ganglioneuroblastoma ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Exogenous administration of gangliosides induced neuronal differentiation with prominent neuritogenesis in human neuroblastoma SH-SY5Y cells in vitro. Neuritogenesis was characterized by ruffling of the cell membrane, the development of lamellipodia and filopodia, and the subsequent elongation and branching of the neurites ultrastructurally. Both axons and neurites were identified. Increased numbers of cell organelles in the neurites and cell bodies were noted. Nonsynaptic contacts and gap junctions formed between neurites or between each neurite and cell body. These findings could be implicated in histopathologic changes from neuroblastoma to ganglioneuroblastoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02478862
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  • 43
    Electronic Resource
    Electronic Resource
    Primary leptomeningeal meningiomatosis with widespread whorl formation (1997)
    Springer
    Brain tumor pathology 14 (1997), S. 139-143 
    Details
    ISSN: 1861-387X
    Keywords: Primary leptomeningeal meningiomatosis ; Meningioma ; Whorl formation ; Immunohistochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a 10-year-old girl with a primary leptomeningeal tumor. She presented with a 5-week history of increased intracranial pressure, progressive cranial nerve deficits, and spinal compression signs. She had previously had a granulosa cell tumor, a benign estrogen-producing ovarian tumor, which was resected 6 months before the initial neurological symptoms developed. At autopsy, the brain and spinal cord showed diffuse neoplastic involvement of the leptomeninges. The tumor was composed of small cells with a high nucleus/cytoplasm ratio, which were immunoreactive for vimentin but not for epithelial membrane antigen or cytokeratin. In addition, the tumor contained many small cellular whorls with desmosome-like junctional complexes between the cells, suggesting that the tumor was a meningioma, basically of the meningothelial type. The term ‘meningeal meningiomatosis’ has been used synonymously with “primary meningeal sarcomatosis”. The present tumor was considered to be a rare example of “meningeal meningiomatosis” of true meningothelial cell origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02478883
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  • 44
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of calcium in rat retina with oxalate pyroantimonate and energy-dispersive X-ray detector (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 76-80 
    Details
    ISSN: 1860-1499
    Keywords: Ultrastructure ; Retina ; Rat ; Oxalate ; Potassium pyroantimonate ; X-ray analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracellular calcium plays an important role in the intracellular signal transduction as one of the second messengers. In this study, we examined the ultrastructural distribution of calcium in rat retina, using the oxalate pyroantimonate technique and X-ray microanalysis. Large amounts of precipitates were observed inside the disc of outer segments of photoreceptor cells (OS) and the synaptic vesicles of the inner (IPL) and outer plexiform layer (OPL). Precipitates also were observed in the ribosome-rich regions in the cytoplasm and the euchromatinic part in the cell nuclei of the ganglion, amacrine, and bipolar and horizontal cells. However, few precipitates were found in the inner segment of the photoreceptor cells and the retinal pigment epithelium (RPE). X-ray microanalysis with an energydispersive X-ray detector revealed that these precipitates had a peak of antimony and calcium. Therefore, it was suggested that these precipitates were Ca[Sb(OH)6]2, the reaction products of the oxalate-pyroantimonate technique. Our findings showed that calcium precipitates are abundant in retinal regions that are related to visual transmission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545085
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  • 45
    Electronic Resource
    Electronic Resource
    An ultrastructural study of oncocytoma and oncocytic carcinoma of the parotid gland (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 31-36 
    Details
    ISSN: 1860-1499
    Keywords: Parotid gland ; Oncocytoma ; Oncocytic carcinoma ; Mitochondria ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One case of oncocytoma and another of oncocytic carcinoma of the parotid gland are reported with ultrastructural studies. The incidence of oncocytoma varies from 0.1% to 1.4% of all parotid gland tumors, while oncocytic carcinoma is extremely rate. The oncocytoma was composed of polyhedral cells with fine eosinophilic granular cytoplasm and a rounded nucleus. The tumor cell clusters were surrounded by basement membrane. The tumor cells of the oncocytic carcinoma were also characterized by eosinophilic cytoplasm, but cellular atypia and mitotic figures were found. Electron microscopically, the cytoplasm of the oncocytoma was packed with abundant mitochondria. They were oval or elongated in shape with stacked cristae. Although the tumor cells of the oncocytic carcinoma also contained many mitochondria, their number was less than that of the benign case, and stacked cristae were very few. Basement membrane was not seen. The ultrastructural characteristics of oncocytoma and oncocytic carcinoma of the parotid gland are discussed with reference to previous reports.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01458349
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  • 46
    Electronic Resource
    Electronic Resource
    Muscle spindles in immobilized muscle: electron microscopic study of recovery (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 102-109 
    Details
    ISSN: 1860-1499
    Keywords: Ultrastructure ; Rats ; Muscle spindles ; Immobilization ; Recovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study is concerned with the morphological recovery process of muscle spindles following a long period of immobilization. The right hindlimbs of rats were fixed with a plaster cast for 4 weeks. Thereafter, four groups of rats were examined by electron microscopy. One group served as the control after the cast was removed. The other three groups were examined after free walking for 4, 8, and 12 weeks, respectively. The muscle spindles (tibialis anterior muscle) of the individual animals were then ultrastructurally analyzed. The morphological alterations (of the outer capsule, intrafusal muscle fibers, and intrafusal nerve components) gradually recovered during free walking and regained almost all normal features in 12 weeks after returning to walking.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01545090
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