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  • 2005-2009  (8)
  • 2000-2004  (14)
  • 1955-1959  (4)
  • 1890-1899
  • Agrobacterium
  • ddc:080
  • 1
    facet.materialart.
    Unknown
    Publication Date: 2019-10-24
    Keywords: ddc:080
    Language: German
    Type: annualzib , doc-type:report
    Format: application/pdf
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  • 2
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    Unknown
    Publication Date: 2019-10-24
    Keywords: ddc:080
    Language: German
    Type: annualzib , doc-type:report
    Format: application/pdf
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  • 3
    Publication Date: 2016-06-09
    Description: Im Rahmen dieser Arbeit wurde ein individuell anpassungsfähiges Modell entwickelt und implementiert, das die Perfusion in menschlichen soliden Tumoren beschreibt und für verschiedene Zeitpunkte w¨ahrend regionaler Hyperthermie ein lokal abhängiges Temperaturprofil berechnet. Da vor jeder Simulation alle wichtigen Parameter anhand von Ultraschall-, MRT- oder Angiogrammbildern individuell bestimmt werden können, wird eine patientenspezifische Aussage ¨uber das intratumorale Antwortverhalten bereits vor der eigentlichen Behandlung möglich. In Abh¨angigkeit von der Qualität der zur Verfügung stehenden anatomischen Daten über das zu simulierende Gebiet kann das Modell beliebig verfeinert oder bei Mangel an detaillierten Informationen auch mit Minimaldaten und reduzierter räumlicher Genauigkeit benutzt werden. Die für eine Simulation benötigten 2- oder 3- dimensionalen Geometrien können leicht mit der am ZIB entwickelten Software Amira erstellt und zur Berechnung in KARDOS, einem ebenfalls am ZIB implementierten Löser für nichtlineare partielle Differentialgleichungen, eingelesen werden. Mit Hilfe dieses Modells wird eine einfache, aber realistische und aussagekräftige Simulation für die Therapieplanung einer klinischen Hyperthermieanwendung ermöglicht, die innerhalb kurzer Zeit vorbereitet und durchgeführt werden kann.
    Keywords: ddc:080
    Language: German
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 4
    Publication Date: 2016-06-30
    Description: In dieser Arbeit werden effiziente Co-Reservierungs-Algorithmen vorgestellt, mit denen Rechenressourcen mehrerer Cluster im Grid reserviert werden können. Es werden fünf Algorithmen vorgestellt, die flexible Co-Reservierungsanfragen ermöglichen. In einer Co-Reservierungsanfrage müssen die angeforderten CPUs der Teilreservierungen exakt festgelegt werden. Die Cluster und die Startzeiten, bei denen die Teilreservierungen durchgeführt werden, ermittelt ein Co-Reservierungs-Algorithmus erst bei der Anfragebearbeitung. Die Benutzer können die Startzeit in der Anfrage eingrenzen, in dem sie eine früheste Startzeit und eine Deadline angeben. In den bisher entwickelten Reservierungsverfahren können Benutzer nur starre Co-Reservierungsanfragen stellen, in denen sie für alle Attribute der Teilreservierungen exakte Werte angeben müssen. Eine flexible Co-Reservierungsanfrage erlaubt einem Grid-Reservierungsdienst optimierte Reservierungsvarianten durchzuführen, da er aus vielen Reservierungsvarianten auswählen kann. Die Anwendungen von lokalen Benutzern können durch die Grid-Reservierungen verzögert werden. Bei der Auswahl der Reservierungsvarianten kann ein Grid-Reservierungsdienst dies berücksichtigen und den negativen Einfluß der Grid-Reservierungen verringern. Bei zwei Co-Reservierungs-Algorithmen werden auch zusätzliche Sortiervorgaben des Klienten bei der Auswahl der Reservierungsvarianten beachtet. Die Algorithmen wurden durch Simulationen mit dem Workload eines realen großen Supercomputers ausgewertet. Die Untersuchungen zeigen, daß sowohl die lokalen als auch die Grid-Benutzer von den flexiblen Anfragen profitieren können. Den lokalen Benutzern wurden die Ressourcen weniger oft durch zu früh gestellte Reservierungsanfragen entzogen. Die Grid- Benutzer können ihrerseits komfortabel eine optimierte Reservierungsvariante durchführen lassen.
    Keywords: ddc:080
    Language: German
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 5
    Publication Date: 2016-06-30
    Keywords: ddc:080
    Language: German
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
    Format: application/pdf
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  • 6
    Publication Date: 2020-08-05
    Keywords: ddc:080
    Language: English
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 7
    Publication Date: 2020-08-05
    Description: In dieser Diplomarbeit werden grundlegende Probleme der kostenoptimalen Dimensionierung von Telekommunikationsnetzwerken untersucht. Diese werden als lineare gemischt ganzzahlige Programme formuliert, wobei sich in der Modellierung auf die Konzepte Routing und Kapazitätszuweisung beschränkt wird. Es werden parallel drei übliche, aus der Praxis motivierte Möglichkeiten behandelt, die auf gerichteten oder ungerichteten Kanten eines Netzwerkes installierte Kapazität zu nutzen. Diese unterscheiden wir als DIrected, BIdirected und UNdirected. Die studierten Probleme treten als Relaxierungen vieler realistischer Fragestellungen der Netzwerkoptimierung auf. Sie enthalten elementare Strukturen, deren Studium ausschlaggebend ist für das Verständnis komplexerer Modelle. Letztere können zusätzliche Erfordernisse berücksichtigen, wie zum Beispiel die Ausfallsicherheit von Netzwerken. Zur Lösung solcher NP-schweren Optimierungsprobleme werden erfolgreich Branch & Bound und Schnittebenenverfahren kombiniert (Branch & Cut). Für die Wirksamkeit dieser Algorithmen ist es sehr nützlich, möglichst genaue Kenntnisse der Struktur der Seitenflächen der zugrundeliegenden Polyeder zu haben, welche die konvexe Hülle der Lösungsmenge beschreiben. Es sind starke gültige Ungleichungen zu finden, welche hochdimensionale Seitenflächen oder sogar Facetten definieren. Diese sollten zudem schnell separiert werden können und die numerische Stabilität der Algorithmen möglichst nicht beeinflussen. Diese Arbeit beschäftigt sich im Wesentlichen mit der sehr allgemeinen Rundungstechnik Mixed- Integer Rounding (MIR) zur Verstärkung gültiger Ungleichungen unter Verwendung der Ganzzahligkeitsnebenbedingungen. Es wird eine MIR-Prozedur motiviert, bestehend aus den Schritten Aggregieren, Substituieren, Komplementieren und Skalieren, welche durch Ausnutzung der Struktur der gegebenen Parameter zu einer gültigen Basisungleichung führt, die dann durch MIR eine starke und oft facetten-induzierende Ungleichung gibt. Es werden verschieden Klassen solcher Ungleichungen untersucht und auf ihre Praxistauglichkeit beim Einsatz in Branch & Cut-Verfahren getestet. Nach einer kurzen Einführung werden in Kapitel 2 die für uns in dieser Diplomarbeit relevanten Probleme definiert. Kapitel 3 gibt eine ausführliche Übersicht über die Technik MIR.Wir beschäftigen uns vor allen Dingen mit den Begriffen Superadditivität und Lifting und behandeln Aspekte wie Numerik und beschränkte Variablen. Kapitel 4 und Kapitel 5 umfassen Untersuchungen zu so genannten cut sets. Diese Polyeder werden durch Schnitte in Netzwerken definiert und relaxieren die von uns behandelten Probleme. Hauptsächlich durch MIR entwickeln wir sowohl neue als auch bekannte Klassen von facetten-definierenden Ungleichungen für cut sets, wobei strukturelle Unterschiede herausgearbeitet werden, die durch die drei verschiedenen Typen der Kapzitätsbereitstellung und durch beschränkte Variablen entstehen. Als ein zentrales Resultat wird bewiesen unter welchen Bedingungen facetten-induzierende Ungleichungen für cut sets auch Facetten der zugehörigen relaxierten Polyeder sind. Im Kapitel 6 geben wir weitere Typen von MIR-Ungleichungen an, die auf anderen Netzwerkstrukturen basieren und weisen ferner auf offene Fragen sowie interessante Ideen hin. Das Kapitel 7 widmet sich schließlich der Entwicklung und Implementation von Separationsalgorithmen. Wir testen einige der entwickelten Ungleichungsklassen mit Hinblick auf Ihre Wirksamkeit zur Lösung von realistischen Problemen der Netzwerkdimensionierung aus der Telekommunikation und diskutieren die Ergebnisse.
    Keywords: ddc:080
    Language: German
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 8
    Publication Date: 2016-06-30
    Description: Diese Arbeit dokumentiert den Entwurf und die Implementierung einer NFS-Schnittstelle zum ZIBDMS. Das ZIBDMS, welches derzeit am Zuse-Institut Berlin entwickelt wird, ist ein Managementsystem für verteilte Daten in heterogenen Umgebungen [10]. Es soll die einfache Benutzung von Mechanismen zur Replikation sowie neue Möglichkeiten des Zugriffs und der Verwaltung von Metadaten und Datenobjekten ermöglichen. Dem Nutzer bietet das System verschiedene Sichten: Neben der klassischen, hierarchischen Sicht mit Verzeichnissen und Dateien wird auch eine attributbasierte Zugriffsmethode angeboten, in der Datenobjekte ausschließlich über Attribut/Wert-Paare referenziert werden. Für den Benutzer transparent, soll sich das System im Hinblick auf die Verfügbarkeit von Daten und Metadaten selbsttätig optimieren. Um Skalierbarkeit gew¨ahrleisten zu können, werden intern Peer-to-Peer- Techniken entwickelt und benutzt. Die NFS-Schnittstelle erhöht die Nutzbarkeit des Systems, da Client- Implementierungen für nahezu alle Betriebssysteme zur Verfügung stehen. Der Benutzer kann so über das gewohnte Dateisystem Daten und Metadaten pflegen, ohne spezifisches Vorwissen zu benötigen. In dieser Arbeit wird untersucht, inwiefern sich die Semantik des ZIBDMS auf jene des NFS-Protokolls übertragen läßt. Weiterhin wird das Konzept von Pseudodateien beschrieben und diskutiert, inwiefern es eine brauchbare Lösung darstellt, den im Vergleich zur NFS-Semantik wesentlich größeren Funktionsumfang des ZIBDMS für den Anwender nutzbar zu machen. Pseudodateien sind nicht-persistente Objekte, welche für den Benutzer als reguläre Datei sichtbar werden und die erweiterte Funktionalitäten wie die Bearbeitung von Metadaten oder die Platzierung von Replikaten über die Dateisystem-Sicht des NFS zur Verfügung stellen sollen. Schließlich werden Leistungsmessungen an den benutzten Komponenten des ZIBDMS vorgenommen und vergleichbaren Systemen gegen übergestellt. Die Aufmerksamkeit liegt hier auf der zugrundeliegenden Katalog-Technologie sowie dem durch das NFS-Protokoll verursachten Overhead.
    Keywords: ddc:080
    Language: German
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 9
    Publication Date: 2020-08-05
    Keywords: ddc:080
    Language: English
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 10
    Publication Date: 2020-08-05
    Description: This diploma thesis deals with the restoration problem in telecommunication networks. The goal is to find a cost minimal capacity capacity assignment on the edges and nodes of a network such that given demands can be satisfied even in case of the failure of an edge or node in the network. Moreover, restrictions on the routing paths (like length restrictions) and hardware constraints have to be satisfied. A Mixed Integer Programming model is presented which takes into account restoration requirements as well as hardware constraints and which abstracts from a particular restoration protocol and failure situation. This abstraction provides new insight into the structure of the network restoration problem and shows that from a mathematical point of view, the commonly used restoration techniques Link Restoration, Path Restoration and Reservation are not as different as they seem to be from a practical point of view. In addition, our model allows (but is not limited to) optimizing working capacity, intended for normal use, and spare capacity, intended for rerouting purposes in case of a failure, in one step. Furthermore, our formulation of capacity cost allows taking into account the effects of discrete, non-linear cost structures which are common in practice. Up to our knowledge, no publication in the existing literature covers all these aspects, let alone in one model, although they are of major practical interest. The model has been implemented in a Branch and Cut framework. The theoretical background of the algorithmic procedure is presented in detail, including computational complexity investigations on the pricing problem. The abstraction from a particular restoration protocol turns out to be useful both from a theoretical and computational point of view. In fact, our investigations suggest a distinction into Local Restoration and Global Restoration rather than into Link Restoration,Path Restoration, Reservation and mixtures of these concepts. In addition to the theoretical aspects of the algorithmic procedure, some implementational details are briefly discussed. Our implementation has been tested on 14 real world instances, which is described in detail. One part of the computational results consists of a comparison of optimal network cost values using diffeent restoration mechanisms, applied to securing either all single node failures, all single edge failures or both. In addition, the effects of a discrete cost structure are investigated, which has rarely been considered yet in literature. Furthermore, the cost ifference between joint and successive working and spare capacity optimization is investigated. In the second part of the computational results, several heuristics for the network restoration problem are compared with respect to both solution quality and time. This diploma thesis deals with the restoration problem in telecommunication networks. The goal is to find a cost minimal capacity capacity assignment on the edges and nodes of a network such that given demands can be satisfied even in case of the failure of an edge or node in the network. Moreover, restrictions on the routing paths (like length restrictions) and hardware constraints have to be satisfied. A Mixed Integer Programming model is presented which takes into account restoration requirements as well as hardware constraints and which abstracts from a particular restoration protocol and failure situation. This abstraction provides new insight into the structure of the network restoration problem and shows that from a mathematical point of view, the commonly used restoration techniques Link Restoration, Path Restoration and Reservation are not as different as they seem to be from a practical point of view. In addition, our model allows (but is not limited to) optimizing working capacity, intended for normal use, and spare capacity, intended for rerouting purposes in case of a failure, in one step. Furthermore, our formulation of capacity cost allows taking into account the effects of discrete, non-linear cost structures which are common in practice. Up to our knowledge, no publication in the existing literature covers all these aspects, let alone in one model, although they are of major practical interest. The model has been implemented in a Branch and Cut framework. The theoretical background of the algorithmic procedure is presented in detail, including computational complexity investigations on the pricing problem. The abstraction from a particular restoration protocol turns out to be useful both from a theoretical and computational point of view. In fact, our investigations suggest a distinction into Local Restoration and Global Restoration rather than into Link Restoration, Path Restoration, Reservation and mixtures of these concepts. In addition to the theoretical aspects of the algorithmic procedure, some implementational details are briefly discussed. Our implementation has been tested on 14 real world instances, which is described in detail. One part of the computational results consists of a comparison of optimal network cost values using different restoration mechanisms, applied to securing either all single node failures, all single edge failures or both. In addition, the effects of a discrete cost structure are investigated, which has rarely been considered yet in literature. Furthermore, the cost difference between joint and successive working and spare capacity optimization is investigated. In the second part of the computational results, several heuristics for the network restoration problem are compared with respect to both solution quality and time.
    Keywords: ddc:080
    Language: English
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 11
    Publication Date: 2020-08-05
    Keywords: ddc:080
    Language: English
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 12
    Publication Date: 2020-08-05
    Description: Mobile cellular communcication is a key technology in today's information age. Despite the continuing improvements in equipment design, interference is and will remain a limiting factor for the use of radio communication. This Ph. D. thesis investigates how to prevent interference to the largest possible extent when assigning the available frequencies to the base stations of a GSM cellular network. The topic is addressed from two directions: first, new algorithms are presented to compute "good" frequency assignments fast; second, a novel approach, based on semidef inite programming, is employed to provide lower bounds for the amount of unavoidable interference. The new methods proposed for automatic frequency planning are compared in terms of running times and effectiveness in computational experiments, where the planning instances are taken from practice. For most of the heuristics the running time behavior is adequate for inter active planning; at the same time, they provide reasonable assignments from a practical point of view (compared to the currently best known, but substantially slower planning methods). In fact, several of these methods are successfully applied by the German GSM network operator E-Plus. The currently best lower bounds on the amount of unavoidable (co-channel) interference are obtained from solving semidefinite programs These programs arise as nonpolyhedral relaxation of a minimum /c-parti tion problem on complete graphs. The success of this approach is made plausible by revealing structural relations between the feasible set of the semidefinite program and a polytope associated with an integer linear programming formulation of the minimum ^-partition problem. Comparable relations are not known to hold for any polynomial time solvable polyhedral relaxation of the minimum ^-partition problem. The appli cation described is one of the first of semidefinite programming for large industrial problems in combinatorial optimization.
    Keywords: ddc:080
    Language: English
    Type: doctoralthesis , doc-type:doctoralThesis
    Format: application/pdf
    Format: application/postscript
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  • 13
    ISSN: 1432-203X
    Keywords: Key words Chickpea ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 mg/l phosphinothricin, depending on the construct used for transformation. Expression of the chimeric GUS gene was confirmed by histochemical localization of GUS activity in regenerated shoots. Resistant shoots were grafted onto 5-day-old dark-grown seedlings, and mature plants could be recovered. T-DNA integration was confirmed by Southern analysis by random selection of putative transformants. The analysis of 4 plantlets of the T1 progeny revealed that none of them was GUS-positive, whereas the presence of the nptII gene could be detected by polymerase chain reaction.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1432-203X
    Keywords: Key words Poplar ; Populus ; Aspen ; Cottonwood ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
    Type of Medium: Electronic Resource
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  • 15
    ISSN: 1432-203X
    Keywords: Key words Allium cepa ; Transformation ; Regeneration ; gfp gene ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. The method takes between 3–5 months from explant to primary regenerant entering the glasshouse. Multiple-shoot formation from primary transgenic material made possible the clonal multiplication of transformants. The binary vector used carried the nptII antibiotic resistance gene and the m-gfp5-ER reporter gene. Transgenic cultures were initially screened for their ability to fluoresce and to grow in the presence of geneticin (5–25 mg/l). The transgenic nature of individual plants was confirmed by Southern blot analysis.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 62 (2000), S. 141-151 
    ISSN: 1573-5044
    Keywords: Agrobacterium ; forage quality ; KDEL ; recombinant proteins ; sulphur-rich amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Accumulation of zeins, the endosperm storage proteins of maize, in a heterologous plant expression system was attempted. Plants of Nicotiana tabacum and Lotus corniculatus were transformed by Agrobacterium with binary vectors harbouring genes that code for γ-zein and β-zein, two zeins rich in sulphur amino acids. Adding the ER retention signal KDEL to the C-terminal domain modified the zein polypeptides. Significant levels of γ-zein:KDEL and β-zein:KDEL were detected in primary transformants of tobacco. Moreover, the two zeins colocalized in leaf protein bodies of γ-/β-zein:KDEL plants derived from a cross between two primary transformants. Coexpression of γ-zein:KDEL and β-zein:KDEL could be a useful strategy to obtain genotypes of forage legumes which are able to accumulate sulphur amino acids to high levels. As a first step, L. corniculatus plants expressing γ-zein:KDEL in the leaves were obtained.
    Type of Medium: Electronic Resource
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  • 17
    ISSN: 1573-5028
    Keywords: Agrobacterium ; binary vectors ; plant transformation ; reporter genes ; selectable marker genes ; Ti vector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids. Its size and copy number in Escherichia coli offers increased efficiencies in routine in vitro recombination procedures. pGreen can replicate in Agrobacterium only if another plasmid, pSoup, is co-resident in the same strain. pSoup provides replication functions in trans for pGreen. The removal of RepA and Mob functions has enabled the size of pGreen to be kept to a minimum. Versions of pGreen have been used to transform several plant species with the same efficiencies as other binary Ti vectors. Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Molecular breeding 6 (2000), S. 185-194 
    ISSN: 1572-9788
    Keywords: Agrobacterium ; transformation ; pea ; Pisum sativum L. ; PCR analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Factors influencing the efficiency of Agrobacterium-mediated transformation of pea were tested using highly efficient, direct regeneration system. The virulence of three Agrobacterium strains (octopine LBA 4404, nopaline C58C1 and succinamopine, hypervirulent EHA 105) clearly varied giving 1 transgenic plant per 100 explants for LBA 4404, 2.2 for C58C1 and 8.2 for EHA 105. To test the efficacy of selection agents we used the hypervirulent EHA 105 strain carrying pGPTV binary vector with one of four different selection genes: nptII, hpt, dhfr or bar. The mean number of transgenic, kanamycin-resistant plants for two cultivars tested was 4.2 per 100 explants and was slightly higher than the number of phosphinothricin-resistant plants (3.6 plants per 100 explants). The proportion of transgenics among kanamycin-selected plants was also higher than among phosphinothricin-resistant plants (35% and 28% respectively). There was no regeneration on hygromycin or methotrexate media (transformation with hpt and dhfr genes). Acetosyringone had no apparent influence on efficiency of transformation with hypervirulent EHA 105 strain, however it did affect the rate of transformation when moderately virulent C58C1 was used. Recovery of transgenic plants was enhanced after application of 5-azacytidine. The presence of integrated T-DNA was checked by PCR and confirmed by Southern hybridization. T-DNA was stably transmitted to the next generation.
    Type of Medium: Electronic Resource
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  • 19
    ISSN: 1572-9788
    Keywords: Agrobacterium ; inheritance analysis ; Lavandula latifolia ; lavender ; organogenesis ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic plants of the aromatic shrub Lavandula latifolia (Lamiaceae) were produced using Agrobacterium tumefaciens-mediated gene transfer. Leaf and hypocotyl explants from 35–40-day old lavender seedlings were inoculated with the EHA105 strain carrying the nptII gene, as selectable marker, and the reporter gusA gene with an intron. Some of the factors influencing T-DNA transfer to L. latifolia explants were assessed. Optimal transformation rates (6.0 ± 1.6% in three different experiments) were obtained when leaf explants precultured for 1 day on regeneration medium were subcultured on selection medium after a 24 h co-cultivation with Agrobacterium. Evidence for stable integration was obtained by GUS assay, PCR and Southern hybridisation. More than 250 transgenic plants were obtained from 37 independent transformation events. Twenty-four transgenic plants from 7 of those events were successfully established in soil. β-glucuronidase activity and kanamycin resistance assays in greenhouse-grown plants from two independent transgenic lines confirmed the stable expression of both gusA and nptII genes two years after the initial transformation. Evidence from PCR data, GUS assays and regeneration in the presence of kanamycin demonstrated a 1:15 Mendelian segregation of both transgenes among seedlings of the T1 progeny of two plants from one transgenic L. latifolia line.
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  • 20
    Publication Date: 2020-08-05
    Description: In der vorliegenden Dissertation untersuchen wir die Optimierung von ausfallsicheren Telekommunikationsnetzwerken. Wir präsentieren unterschiedliche gemischt-ganzzahlige Modelle für die diskrete Kapazitätsttruktu,, sowie für die Sicherung des Netzes gegen den Ausfall einzelner Komponenten. Die Modelle wurden in einer Kooperation mit der E-Plus Mobilfunk GmbH verwendet. Die theoretischen Resultate wurden in Algorithmen umgesetzt und in das von uns entiickllte Netzwerksoptimierungswerkzeug Discnet (Dimensioning Survivable Capaiitated NETworks) integriert, welches seit mehreren Jahren in der Planung bei E-Plus eingesetzt wird. Wir betrachten das Transportnetzllanungsproblem eines Telekommunikationsanbieters. Dieses Problem setzt auf logischen Kommunikattonsanforerrungen zwischen den Standorten (Knoten) des zu planenden Netzes und potentiell inslallirrbaren Verbindungen (Kanten) zwischen derselben Knotenmenge auf. Ein Kapazitätsmodell stellt die Information bereit, welche Kapazitäten auf den potentiellen Kanten verfügbar sind. Wir betrachten zwei Modelle. Entweder ist eine explizite Liste der verfügbaren Kapazittten gegeben oder eine Menge von sogenannten Basiskapazitäten, die auf jeder Kante indiviuelll kombiniert werden können. Die Basiskapazitäten müßen paarweise ganzzahlige Vielfache voneinander sein. Man beachte, daß diese Eigenschaft von den internationalen Standards PDH und SDH erfüllt wiid. Ein Ausfallsicherheitsmodell stellt die Information bereit, wie das zu planende Netz gegen den Ausfall einzelner Netzkomponenten geschützt werden soll. Wir betrachten sinnvolle Kombinationen der Modelle Diversification, Reservation und Path Restoration. Das erste Modell garantiert Ausfallsicherheit durch kommunikationsbedarfsabhängige Beschränkung des Prozentsatzes, der durch einzelne Netzkomponenten geroutet werden darf. Bei den beiden anderen Modelle können Kommunikationsbedarfe bei Ausfall einer Netzkomponente auf unterschiedliche Weise neu geroutet werden. Ziel der Planung ist eine ktstenminimlle Kapatitätsentscheidung, die eine Routenllanung aller Kommunikationsbedarfe gemäß den Ausfallsicherheitsanforderungen ermöglicht. Wir entwickeln ein Schnittebenenverfahren zur Lösung der betrachteten Optimiergngsrrobleme. Zu diesem Zweck untersuchen wir Polyeder, die mit den verschiedenen Problemen assoziiert sind. Wir präsentieren neue Klassen von Ungleichungen, entwickeln Separationsalgorithmen und Heuristiken. Mit dem Schnittebenenverfahren werden untere und obere Schranken für den Wert von Oitimallösungen berechnet, und daher ist es möglich, Qualitätsgarantien für die berechneten Löungen anzugeben. Parallel zur Beschreibung der implementierten Algorithmen präsentieren wir umfangreiche Tests mit praktisch relevanten Daten, die zu Problemen mit mehr als 2 Billionen Variablen führen.
    Keywords: ddc:080
    Language: English
    Type: doctoralthesis , doc-type:doctoralThesis
    Format: application/pdf
    Format: application/postscript
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  • 21
    Publication Date: 2020-08-05
    Keywords: ddc:080
    Language: English
    Type: doctoralthesis , doc-type:doctoralThesis
    Format: application/pdf
    Format: application/postscript
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  • 22
    Publication Date: 2020-08-05
    Keywords: ddc:080
    Language: German
    Type: doctoralthesis , doc-type:doctoralThesis
    Format: application/pdf
    Format: application/postscript
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  • 23
    ISSN: 1573-5060
    Keywords: Agrobacterium ; Brassica napus ; CaMV 35S promoter ; mas promoter ; gene expression ; risk assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Gene fusions between the β-glucuronidase (GUS) reporter gene and the promoters of the cauliflower mosaic virus 35S RNA transcript (CaMV 35S) and the mannopine synthase (mas) genes were introduced into rapeseed varieties via Agrobacterium-mediated transformation. Fluorometric assay of β-glucuronidase activity indicated different expression patterns for the two promoters. In seedlings, the CaMV 35S promoter had maximum activity in the primary roots, while the mas promoter was most active in the cotyledons. Etiolated seedlings cultured in the dark showed reduced activity of the mas promoter. Before vernalization at the rosette stage, both promoters were more active in older plant parts than in younger ones. At this stage the highest activity was recorded in cotyledons. After the plants had bolted reduced promoter function was detected in the upper parts of the transformed plants. Both promoters were found to be functional in the majority of the studied organs of transgenic rapeseed plants, but the promoter activity varied considerably between the organs at different developmental stages. The ability of pollen to transfer the introduced genes to other varieties and related species (e.g. Brassica napus and Diplotaxus muralis) by cross-pollination was studied in greenhouse experiments, and field trials were carried out to estimate the distance for biologically-relevant gene dispersal. In artificial crossing, the introduced marker gene was transferable into other varieties of Brassica napus. In field trials, at a distance of 1 metre from the source of transgenic plants, the frequency of an outcrossing event was relatively high (10-3). Resistant individuals were found at 16 and 32 metres from the transgenic pollen donors, but the frequency of an outcrossing event dropped to 10-5.
    Type of Medium: Electronic Resource
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  • 24
    ISSN: 1573-5060
    Keywords: Agrobacterium ; transformation ; lily ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Lily cv. Harmony was inoculated with several Agrobacterium strains to study its susceptibility to Agrobacterium infection and transformation. Tumorous tissue formation on inoculated stem internodes of sterile-grown plantlets, as well as expression of a β-glucuronidase marker gene interrupted by an intron in cells of inoculated stem nodes, indicate that the monocotyledon Lilium is a host for Agrobacterium.
    Type of Medium: Electronic Resource
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  • 25
    ISSN: 1573-5060
    Keywords: Agrobacterium ; plant regeneration ; potato ; Solanum tuberosum ; tissue culture ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To provide a truly genotype-independent transformation system, it is necessary to be able to transform a wide range of potato genotypes. The ability to regenerate shoots in vitro was determined for 34 potato varieties using tuber disc explants. Following a culture regime used extensively in previous studies with the variety Desiree, half of the varieties could be regenerated from tuber discs and half could not. From a sample of varieties that could be regenerated from tuber discs, all but one variety gave transgenic plants. Twelve varieties were evaluated for the capacity to regenerate shoots from leaf and internode explants excised from in vitro grown plants. All of the varieties tested regenerated adventitious shoots. Leaf and internode explants from 5 varieties were subsequently used for transformation, and transgenic plants were produced from two potato varieties that did not give transgenic plants from tuber disc explants. Some varieties could not be transformed by either method, and will require modification of the in vitro regeneration and transformation system to be successful.
    Type of Medium: Electronic Resource
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  • 26
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 85 (1955), S. 131-134 
    ISSN: 1573-5060
    Keywords: apple ; transformation ; Agrobacterium ; preculture ; azacytidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf explants of apple cvs Gala and Golden Delicious were infected with the Agrobacterium tumefaciens strain AGL0(pMOG410). The effects of a 2 d preculture of the explants before infection and the addition of 5-azacytidine to the selection medium were studied. The percentages of GUS-positive explants after 5 w did not significantly alter due to these treatments. One of the ‘Gala’ shoots, which was removed from a leaf explant cultured for 8 w on selection medium, proved to be GUS-positive and will be analyzed further. In general, however, it should be concluded that regeneration of transgenic shoots directly from leaf tissue was not very effective.
    Type of Medium: Electronic Resource
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