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  • 1
    Electronic Resource
    Electronic Resource
    GABA Is an Afferent Vestibular Neurotransmitter in the Guinea Pig: Immunocytochemical Evidence in the Utricular Maculae (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 656 (1992), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1992.tb25302.x
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  • 2
    Electronic Resource
    Electronic Resource
    Two Forms of Rat Brain Glutamic Acid Decarboxylase Differ in Their Dependence on Free Pyridoxal Phosphate (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 44 (1985), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: There are two forms of glutamate decarboxylase (GAD) found in the rat brain. One form (form A) does not require exogenous pyridoxal-5′-phosphate (PLP) for activity whereas another form (form B) requires exogenous PLP for activity. These two forms differ greatly in temperature sensitivity, inactivation, and reactivation by the removal and readdition of PLP, electrophoretic mobility, and regional distribution. For instance, forms A and B are inactivated to an extent of 91% and 10%, respectively, by the treatment at 45°C for 30 min; form A is greatly inactivated (77%) by the removal of PLP by aminooxyacetic acid and the readdition of PLP, whereas form B is only slightly inactivated (7%). Forms A and B can be clearly separated by 5% polyacrylamide gel electrophoresis in which form A migrates faster than form B. In all 10 brain regions studied, form A is present in smaller amounts than form B. This difference is greatest in the superior colliculus (the ratio of B to A is about 5), while in the locus coeruleus and cerebellum, forms A and B are present in nearly equal proportion. Forms A and B are similar with respect to relative abundance in hypotonie, isotonic, and hypertonic preparations, inhibition of catalytic activity by a carbonyl-trapping agent, immunochemical properties, and chromatographic patterns in a variety of systems. The significance of forms A and B and PLP in the regulation of γ-aminobutyric acid (GABA) level is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1985.tb12910.x
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  • 3
    Electronic Resource
    Electronic Resource
    Short-time Breadmaking Systems. Effect of Formulation, Additives, Temperature, and Flour Quality (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An optimum formula for short-time breadmaking included flour (100%), yeast (5%), sugar (3%), salt (1.5%), shortening (3%), oxidant (100 ppm ascorbic acid and 60 ppm KBrO3), and optimum water. The minimum fermentation time for good volume was 15 min. Ambient temperature greatly affected baking performance, and formula changes would be required to produce optimum short-time bread at different temperatures. Baking the same flours by both short- and long-time baking methods gave poor correlations between loaf volumes and oxidant requirements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb07750.x
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  • 4
    Electronic Resource
    Electronic Resource
    RHEOLOGICAL CHANGES IN CRACKER SPONGES DURING the FIRST FIVE HOURS of FERMENTATION (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food processing and preservation 14 (1990), S. 0 
    Details
    ISSN: 1745-4549
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Reconstitution and interchange studies of gluten, starch, and water soluble fractions from no- and 5-h fermentation sponges showed that gluten was altered and was responsible for the early rheological changes in cracker sponges. It was also shown that breaking of disulfide bonds was responsible for those changes. the “slurry” or inoculum was found to have the greatest effect on rheological properties. Chemical agents, such as sodium bisulfite, cysteine, and glutathione, were effective in reducing the resistance-to-extension of cracker sponges at higher pH levels. However, those effects were eliminated by the lactic acid produced in the sponge during fermentation. Therefore, the changes in rheology occurring early in fermentation do not appear to be important in cracker baking.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4549.1990.tb00122.x
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  • 5
    Electronic Resource
    Electronic Resource
    Glutamic acid production in an airlift reactor with net draft tube (1992)
    Springer
    Bioprocess and biosystems engineering 8 (1992), S. 183-187 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cultivation of Brevibacterium divaricatum for glutamic acid production in an airlift reactor with net draft tube was developed. Cell concentration gave an index for adding penicillin G. On-line estimation of total sugar concentration yielded an identified model which was used for determination of the substrate addition. Fermentation for glutamic acid production requires high oxygen concentration in the broth. The proposed reactor has the capability to provide sufficient oxygen for the fermentation. Since the reactor is suitable for fed-batch culture, the cultivation of B. divaricatum for glutamic acid production in the proposed reactor is successfully carried out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01254235
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  • 6
    Electronic Resource
    Electronic Resource
    The compensatory ‘rebound’ of reactive astrogliosis: glial fibrillary acidic protein immunohistochemical analysis of reactive astrogliosis after a puncture wound to the brain of rats with portocaval anastomosis (1991)
    Springer
    Acta neuropathologica 82 (1991), S. 72-77 
    Details
    ISSN: 1432-0533
    Keywords: Reactive astrogliosis ; Portocaval anastomotic encephalopathy ; Puncture wound ; Compensatory and decompensatory phases of Alzheimer II gliosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was designed to compare the degree of reactive astrogliosis occurring around a puncture wound in the brain of normal rats and at different intervals after a similar puncture wound in rats with a portocaval anastomosis. The gliosis was evaluated by the number of astrocytes, the thickness of their processes and the intensity of the glial fibrillary acidic protein immunoreactivity. After the puncture wound in the brain of rats with a portocaval anastomosis, the gliosis varied at different intervals being: (1) decreased at 10 days, (2) markedly increased at 5 weeks and (3) significantly decreased at 8, 12, and 16 weeks. These findings suggest that 5 weeks after portocaval anastomosis, an active proliferation of the metabolically altered astrocytes occurs with heightened synthesis of glial fibrillary acidic protein in the period of adaptive compensation, the so-called compensatory ‘rebound’. At 8 weeks or more after portocaval anastomosis, these altered astrocytes were considered to be in the phase of decompensation and incapable of maintaining the reactive response which occurred in normal rats. The compensatory rebound and decompensatory ‘decline’ illustrate the dynamic plasticity of the reactive astrogliosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310926
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  • 7
    Electronic Resource
    Electronic Resource
    Co-release of glutamate and aspartate from cholinergic and GABAergic synaptosomes (1987)
    [s.l.] : Nature Publishing Group
    Nature 330 (1987), S. 64-66 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Whole synaptosome fractions from cerebral cortex were incubated with non-immune (as control) or immune serum to allow specific immunoglobulins to attach to the synaptosomal surface as appropriate. Labelled synaptosomes were then exposed to magnetic microspheres to which protein A had been ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/330064a0
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  • 8
    Electronic Resource
    Electronic Resource
    Optical monitoring of activity of many neurons in invertebrate ganglia during behaviors (1988)
    Springer
    Cellular and molecular life sciences 44 (1988), S. 369-376 
    Details
    ISSN: 1420-9071
    Keywords: Voltage-sensitive dye ; abdominal ganglion ; buccal ganglion ; neuronal activity ; gill-withdrawal reflex ; Aplysia ; Navanax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Optical methods for monitoring neuron activity were developed because these methods lend themselves to simultaneous multiple-site measurements. With the use of new voltage-sensitive dyes, the dye-related pharmacology and photodynamic damage appear to be relatively unimportant. Using multiple-site measurements made with a 124-element photodiode array, we estimated that approximately 30 of the 200 neurons present in theNavanax buccal ganglion make action potentials during feeding and that approximately 300 of the 1100 neurons present in theNavanax buccal ganglion make are active during the gill-withdrawal reflex. The fact that a light mechanical touch to the siphon skin activated such a large number of neurons in the abdominal ganglion suggests that understanding the neuronal basis of the gill-withdrawal reflex and its behavioral plasticity may be forbiddingly difficult.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940529
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  • 9
    Electronic Resource
    Electronic Resource
    GABA and GAD-like immunoreactivity in the primate retina (1987)
    Springer
    Histochemistry and cell biology 86 (1987), S. 485-490 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary GABA immunoreactivity was studied and compared with GAD immunoreactivity in the retinae of baboon, cynomolgus monkey and man. The central and peripheral parts of the retinae were investigated separately in cynomolgus monkey and in man. The same kinds of structures were stained with both antisera. Cells with a position corresponding to amacrine cells were stained, as well as processes in the inner plexiform layer and some cells in the ganglion cell layer. The outer plexiform layer and some cells with the position and configuration of horizontal cells also appeared immunoreactive. Staining was also observed in bipolar-like cells, in man most clearly when using the GABA antiserum in sections from the central parts of the retina. It is possible that horizontal cells, as well as bipolar-like cells, may play a previously unsuspected role in GABAergic transmission in the primate retina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500621
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  • 10
    Electronic Resource
    Electronic Resource
    Possible coexistence of amino acid (γ-aminobutyric acid), amine (dopamine) and peptide (substance P); neurons containing immunoreactivities for glutamic acid decarboxylase, tyrosine hydroxylase and substance P in the hamster main olfactory bulb (1988)
    Springer
    Experimental brain research 71 (1988), S. 633-642 
    Details
    ISSN: 1432-1106
    Keywords: Coexistence ; GABA ; Catecholamine ; Substance P ; Olfactory bulb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The coexistence of immunoreactivities for glutamic acid decarboxylase (GAD), tyrosine hydroxylase (TH) and substance P (SP) was revealed in the hamster main olfactory bulb, using the peroxidase-antiperoxidase immunohistochemical method. Adjacent 40 μm thick Vibratome sections were incubated in different antisera and those cells which were bisected by the plane of sectioning were identified at the paired surfaces of two consecutive sections. The coexistence of the immunoreactivities for 1) TH and GAD, 2) TH and SP and 3) GAD and SP in the same cells could thus be determined by observing the immunoreactivity of the two halves of the cell incubated in two different antisera. About 70% of TH-like immunoreactive (TH-LI) neurons in the periglomerular region also contained GAD-like immunoreactivity, whereas about 45% of GAD-LI ones were also TH-like immunoreactive. Furthermore, almost all (more than 95%) of SP-LI neurons contained both GAD-like and TH-like immunoreactivities. These observations indicate that in the periglomerular region of the hamster main olfactory bulb, some neurons (about 9% of all neurons containing TH-like and/or GAD-like immunoreactivities) may contain three different categories of neuroactive substances, that is, amino acid (GABA), amine (dopamine) and peptide (SP).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248757
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