ISSN:
1432-0797
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A mathematical model for the design of bubble-columns for growth of shear-sensitive insect cells is presented. The model is based on two assumptions. First, the loss of cell viability as a result of aeration is a first-order process. Second, a hypothetical volume X, in which all viable cells are killed, is associated with each air bubble during its lifetime. The model merely consists of an equation for k d, the first-order death-rate constant, and A min, the minimum specific surface area of the air bubbles to supply sufficient oxygen. In addition to X, the equation for k d contains the air flow F, the air-bubble diameter d b, the diameter D and the height H of the bubble column. This equation has been experimentally validated. Comparison of the equations for k d and A min shows that especially H is the key parameter to manipulate in bubble-column design in order to meet the demands set by A min and k d〈kg, the first-order growth-rate constant. It is concluded that net growth of cells is enhanced as size and height of the bubble column increase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00372858
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