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  • 1
    Digitale Medien
    Digitale Medien
    BaM/Pt multilayered films with fine grains and large perpendicular magnetic anisotropy for high density recording media (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 89 (2001), S. 6837-6839 
    Details
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: A magnetoplumbite type of Ba ferrite (BaM) thin layer was deposited on a 9 nm thick Pt underlayer, and excellent c-axis orientation was observed even for an 8 nm thick BaM layer, which corresponds to only three or four BaM unit cells. The grain size was almost in the same range of 60–85 nm even when the BaM layer thickness tBaM decreased from 60 to 17 nm, and tBaM should be reduced below 10 nm to make a grain size smaller than 50 nm. However, the perpendicular coercivity Hc⊥ and squareness S⊥ decreased drastically from 2.6 to 0.5 kOe and from 0.6 to 0.2, respectively, with the decrease of tBaM from 60 to 8 nm because of higher demagnetizing field and susceptibility to thermal fluctuation. On the other hand, the [BaM(5–24 nm)/Pt(9 nm)]3 multilayer exhibited higher Hc⊥ and larger S⊥ than the BaM/Pt bilayer for the same BaM layer thickness and Hc⊥ and S⊥ of the [BaM(8 nm)/Pt(9 nm)]3 multilayer was 2.0 kOe and 0.6, respectively. It was clarified that the deposition of the BaM/Pt multilayer was very effective for achieving a high perpendicular magnetic anisotropy constant even for the ultrathin BaM layer. © 2001 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1359468
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  • 2
    Digitale Medien
    Digitale Medien
    Observation of resonant optical–phonon assisted tunneling in asymmetric double quantum wells (1998)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 83 (1998), S. 962-965 
    Details
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Laser Raman microscope measurements in asymmetric double quantum wells with coupled narrow and wide quantum wells were performed to observe the nonequilibrium longitudinal-optical (LO) phonons that are generated by electrons during the phonon assisted tunneling. Both the Stokes and the anti-Stokes intensities show maxima at a certain applied voltage, where the calculated subband spacing between the wide and the narrow quantum well states is found to be equal to the LO phonon energy. This fact indicates that the population of nonequilibrium LO phonons becomes maximum when resonant LO phonon scattering occurs. A strong reduction in the photoluminescence intensity for the narrow quantum well is also observed at the same bias condition. © 1998 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.366784
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  • 3
    Digitale Medien
    Digitale Medien
    Mouse-related dermatosis (2004)
    Oxford, UK : Blackwell Science Ltd
    International journal of dermatology 43 (2004), S. 0 
    Details
    ISSN: 1365-4632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-4632.2004.02299.x
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  • 4
    Digitale Medien
    Digitale Medien
    Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 239 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: We previously designed a triple auxotrophic host-vector system in Aspergillus oryzae by isolating red-colored adenine auxotrophic mutants upon UV mutagenesis of a double auxotrophic host (niaD−sC−). In the present study an effort to exploit this system and construct a novel quadruple auxotrophic host was made by disrupting the argB gene involved in arginine biosynthesis. The argB gene-disruption cassette was generated by fusion PCR, which required only two steps of PCR to insert the selectable marker, adeA, into the target argB gene. The chimeric DNA fragment was transformed into the triple auxotrophic strain (niaD−sC−adeA−) and the argB disruptants were obtained with a high rate of efficiency (approximately 40%). The argB disruptants were characterized by normal colony color and reversal of arginine auxotrophy by introduction of the wild-type argB gene. Quadruple auxotrophic strains (niaD−sC−ΔargB adeA− or niaD−sC−ΔargB adeB−) were subsequently isolated upon UV mutagenesis of the triple auxotrophic strain (niaD−sC−ΔargB) followed by screening of red-colored colonies for adenine auxotrophy. The results obtained showed that the adeA gene served as an efficient selection marker in developing a novel host-vector system with quadruple auxotrophy in A. oryzae, thus providing a powerful tool to breed multiple auxotrophic mutants from a deuteromycete wherein sexual crossing is impossible.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/j.femsle.2004.08.025
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  • 5
    Digitale Medien
    Digitale Medien
    A HU-like gene mutation in Rhizobium leguminosarum bv. viciae affects the expression of nodulation genes (2004)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 51 (2004), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: NodD is the major regulator of nod genes expression in rhizobia. Previously, a HU-like protein in Rhizobium leguminosarum bv. viciae has been identified to bind specifically with nod promoters and be involved in in vitro nodD transcription, but its in vivo function remained unknown. In this work we have cloned and sequenced the R. leguminosarum bv. viciae gene, named hurL, for this HU-like protein. Using the E. coli-expressed HurL proteins, we proved that HurL had high affinity to several nod promoters and showed a stimulation effect on in vitro nodD transcription at appropriate concentration. The R. leguminosarum bv. viciae hurL gene was mutated by insertion of a kanamycin resistance cassette. The obtained hurL mutant strain M704 exhibited poor growth under free-living conditions and failed to induce nodules on Pisum sativum cv. Frisson and Vicia hirsuta. Further studies of NodD production and nod genes-lacZ fusions expression in the hurL mutant revealed that inactivation of hurL led to severe impairment in the nodD expression, repression in the inducible expression of nodA and nodF, and slight enhancement in the expression of px2, a gene identified earlier in this lab. These results suggested that hurL might be required for maintaining the normal expression of nod genes in R. leguminosarum bv. viciae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2958.2003.03873.x
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  • 6
    Digitale Medien
    Digitale Medien
    Analysis of the Load-Carrying Capability of the Casing Plug Joint of Steel Tube Structures Considering the Contact Effect (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 33-37 (Mar. 2008), p. 321-326 
    Details
    ISSN: 1662-8985
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Notizen: Nonlinear finite element model analysis of the casing plug joints of steel tubular has beenrealized by ANSYS software. The law of load-carrying capability and stiffness of joint are separatelygained by changing the ratio of length and diameter (R/L) and the ratio of the casing length and themain tube length (l/L). The influence of the casing thickness on the load-carrying capability andstiffness are also discussed. The results indicated that the load-carrying capability and stiffness of thejoints both increase with the ratio(R/L) increment and the ratio of the casing length and main tubelength (l/L). When the main tube thickness is equal to casing thickness, the load-carrying capacity ofjoints achieves the most
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.33-37.321.pdf
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  • 7
    Digitale Medien
    Digitale Medien
    Study for Laser Cladding of Ni60A on Copper Substrate by Laser (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 392-394 (Oct. 2008), p. 125-130 
    Details
    ISSN: 1013-9826
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Notizen: The Ni60A powder was clad on the copper by the laser through coaxial power feeding.The hardness and erosion resistance of the substrate and the cladding layer were measured andmade contrasts. The morphology and the microstructures of the cladding layer were analyzed byscanning electronic microscopy as well as the microstructure of bonding interface. The contents ofmajor elements in the bonding interface were studied by energy dispersive spectrometer. Moreover,large amount of (Cu and Ni) solid solution formed a metallurgic and tight bonding with the gooddilution between the substrate and cladding layer
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.392-394.125.pdf
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  • 8
    Digitale Medien
    Digitale Medien
    In vitro Investigation of Mesenchymal Stem Cells with Nanophase PLGA/HA Composite (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1153-1156 
    Details
    ISSN: 1013-9826
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Notizen: Bone grafts have been used to fill bone defects caused by disease or trauma. The amount ofautografts is limited and allogenic bone grafts may transmit diseases and cause immune responses.Numerous materials have been proposed and used as scaffolds for bone tissue reconstruction. In thisstudy, we tested nanophase PLGA/HA composite with mesenchymal stem cells in vitro to examineits biological response and cellular activity. The nanophase composite was compared toconventional polystyrene on cytocompatibility by cell attachment, proliferation, alkalinephosphotase activity test and scanning electron microscopy (SEM) analysis. The resultsdemonstrated that human mesenchymal cells showed more cell attachment and higher cellproliferation rate when growing on nanophase PLGA/HA composite than those growing onpolystyrene alone. And the composite also promoted MSC cells differentiate to osteoblast cells ascompared with control. It was suggested that the combination of bone marrow mesenchymal cellswith artificial materials or differentiation factors may enhance bone formation and regeneration,nanophase PLGA/HA composite might therefore be a promising scaffold material for bone tissuesubstitute in clinical application
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1153.pdf
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  • 9
    Digitale Medien
    Digitale Medien
    The Osteogenic Potential of Stem Cell- and BMP2 Gene-Activating Bioceramic In Vivo (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 284-286 (Apr. 2005), p. 941-944 
    Details
    ISSN: 1013-9826
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Notizen: Stimulation of bone healing through local application of growth factors from implantsmay improve the clinical outcome in fracture treatments. However, the growth factors in reconstructive application require supraphysiologic dosing and considerable expense while hampering their clinical application. Genetic modification of mesenchymal stem cells (MSCs) to both produce and respond to osteogenic factors may have potential for use in enhancing bone healing. In this study, MSCs were genetically modified by a recombinant adenoviral containing the gene for human bone morphogenetic protein 2 (hBMP-2). The gene-transduced cells wereincorporated with a porous beta-tricalcium phosphate (TCP) as a novel complex. We investigated osteogeneic potential of gene-transduced MSCs/ceramic and the ability of the complex on facilitating bone formation in a radius segmental defect of rabbits. In vitro results showed that there were apparent hBMP2 gene expression and protein synthesis in MSCs with hBMP2 stably transfection, whereas negative expression of hBMP2 in controls. Histological studies demonstrated that gene-transfected MSCs/ceramic composite appeared an ability of heterotopic osteogenesis. In the segmental bone defects, endochondrial ossification at fracture sites was found in both transfected and untransfected MSCs-ceramic composites. While the composite with hBMP2 transfection showed the earliest and the most effective healing of the segmental bone defects both radiographically and morphologically. Our results show that genetically modified MSCs/ceramicshad enhanced osteogeneic capacity relative to unmodified MSCs or only ceramic implants. This study suggests that use of cell-and gene-activated bioceramics may offer promise for molecular design of implants to induce osteogenesis and enhance bone regeneration
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.284-286.941.pdf
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  • 10
    Digitale Medien
    Digitale Medien
    Nano-Calcium Phosphate/PLLA Incorporated with bFGF as In Situ-Transplantable Carrier for Cartilage Regeneration (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 309-311 (May 2006), p. 953-956 
    Details
    ISSN: 1013-9826
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Notizen: A novel composite of biodegradable Poly-L-lactic acid (PLLA) with the deposition of the nanosized amorphous calcium phosphate (NCP) particles was developed as tissue engineering scaffold. To improve the minor intrinsic healing capacity of cartilage tissue, the porous composite with desired degradation rate was incorporated with basic fibroblast growth factor (bFGF) andevaluated in the in vivo environment. Full-thickness defects were created in the weight-bearing surface of the femoral condyles in a rabbit model. The defect was filled with and without NCP/PLLA scaffold as a carrier of bFGF. Gross morphology for the test implant showed that the defect was filled with regenerated tissue. It resembled cartilaginous tissue and restored the contour of the condyle at 8 weeks after operation. For the untreated control, no cartilage-like tissue wasobserved at all defects. Histological analysis revealed neochondrogenesis and clusters of cartilaginous extracellular matrix observed with safranin-O staining at 4 weeks for the NCP/PLLA with bFGF treated defects. At 8 weeks after operation, well-formed and mature cartilage was resurfaced the defects. While only fibrous tissue replacement was observed for the control either at4 or 8 weeks. Special staining for cartilage indicated the presence of highly sulfatedglycosaminoglycans and collagen, which were the major extracellular matrices of cartilage. This investigation showed the potential of NCP/PLLA loaded with bFGF in the study of in situ-transplantable carrier to improve healing of cartilage tissue lesion
    Materialart: Digitale Medien
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/51/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.309-311.953.pdf
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