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  • Electronic Resource  (364)
  • 2020-2022
  • 1980-1984  (364)
  • Life Sciences
  • 101
    Electronic Resource
    Electronic Resource
    Production of neutral and alkaline proteases in batch and chemostat cultures by bacillus mesentericus 76 (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 221-225 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the bacterial extracellular protease synthesis (neutral and alkaline protease of Bacillus mesentericusstrain 76, R-form) in batch and chemostat cultures under conditions of glucose limitation were investigated. When the medium was supplemented with casein the production of the proteases was significantly higher. Optimal dilution rates for obtaining of two proteases are fixed. The synthesis of both alkaline and neutral proteases is controlled by catabolite repression and induction.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030304
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  • 102
    Electronic Resource
    Electronic Resource
    Metabolic Features of Methane- and Methanol-Utilizing BacteriaSymposium on Biotechnology 1982, Leipzig, G. D. R. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 269-277 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Investigations of a wide range of methane- and methanol-utilizers showed a striking versatility of their metabolism dependent on the genotype and growth conditions. A correlation between pathways of carbon and nitrogen metabolism was found. It was most stringent in obligate methane-utilizers: the hexulosephosphate pathway bacteria assimilated NH3 by the reductive amination of α-ketoglutarate or pyruvate whereas the serine pathway bacteria used the glutamate cycle (glutamine synthetase + glutamate-oxoglutarate aminotransferase).Multiple enzymic lesions were found in central metabolism of obligate methylotrophs, i.e. the absence of the enzymes of glycolytic and pentosephosphate pathways, gluconeogenesis, citric acid cycle and glyoxylate shunt. These metabolic blocks were not so profound and could be compensated in restricted and facultative methylotrophs during heterotrophic growth.The average levels of exogenous CO2 fixation in methylotrophic bacteria with the hexulosephosphate, serine and ribulosebisphosphate pathways were found to be 10, 30 and 80% of their total cell carbon, respectively.These results served as a basis for biotechnological applications of metabolic potential of methylotrophs (production of biomass, polysaccharides and enzymes as well as for microbiological treatment of industrial waters containing toxic C1- and Cn-compounds).
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030311
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  • 103
    Electronic Resource
    Electronic Resource
    Russian Article pp. 309-318 (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 309-318 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is demonstrated that aliphatic hydrocarbons (alkanes) penetrate into bacteria cells by the way of passive diffusion. The mechanism of this process is different for several bacteria species. A hydrophobic cell wall is essential for that process. In saprophytic Mycobacteria hydrocarbons are solubilized in the thick hydrophobic cell wall. During the process of absorption hydrocarbons pass through the whole cell wall up to the membrane. In the case of Arthrobacteria the hydrocarbons might pass not through the whole cell wall, but through special lipophilie canals.Mobile hydrocarbon-oxidizing bacteria g. Pseudomonas form peptidoglycolipid and excrete it into the medium. The peptidoglycolipid emulsifies hydrocarbon substrate.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030403
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  • 104
    Electronic Resource
    Electronic Resource
    Sugar compositions of extra- and intracellular polysaccharides produced by candida lipolytica (4124) grown on n-hexadecane (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 59-64 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The free monosaccharide content of C. lipolytica (strain 4 124) cells grown on n-hexadecane was identified and found to be only glucose. The chromatographic analysis of the hydrolysate of intracellular cell wall polysaccharides indicated the presence of glucose: mannose: galactose: xylose in a ratio of 1 : 1.32 : 1.07 : 0.35.Paper and dise electrophoresis of extracellular polysaccharid from the culture broth was found to be heterogeneous.Ethanol fractionation separated it to a major component F (I) 81.99% and a minor one F (II) 13.04%.Analysis of the major fraction showed that it consisted of galactose and mannose only while the minor polysaccharide consisted of galactose, glucose and mannose.Thus it was concluded that the predominant sugar in both extracellular and intracellular polysaccharides was mannose. Xylose was detected in the intracellular polysaccharide only.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030115
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  • 105
    Electronic Resource
    Electronic Resource
    Zur Bedeutung der wahren maximalen spezifischen Wachstumsrate (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 76-78 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In this report the biokinetic parameter μmax, m and Ymax for the denitrification of a medium containing acetat as C-source and a soft coal gasification water containing volatile fatty acids as main components have been compared. An expression for a true maximum specific growth rate is proposed (μmaxw = μmax + mYmax) and it could be shown that despite different values for μmax the values for μmaxw correspond fairly well.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030118
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  • 106
    Electronic Resource
    Electronic Resource
    Preliminary results in the fluorometric investigation of fermentation processes (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 79-81 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A flow cuvette for fluorescence exciting and measuring directly on the surface of fermentation broths was developed. Compared with the commonly used cuvette the disturbances by inhomogeneities of the samples and by the inner filter effect could be lowered to an appreciable extent.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030119
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  • 107
    Electronic Resource
    Electronic Resource
    A new biometrical screening method as applied to nutrient optimization in fermentationPaper given at the 2nd Symposium of Socialist Countries on Biotechnology, Leipzig 2.-5. 12. 1980. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 89-92 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030121
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  • 108
    Electronic Resource
    Electronic Resource
    Heat flow measurements in aerobic microbial growth processes with a nonisothermal calorimeter operating directly in the fermenterPresented on the International Bio-Calorimetry Symposium sponsored by the IUPAC, Tbilisi, USSR, September 1981. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 83-87 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A nonisothermal flow calorimeter operating directly in the fermenter was used for heat flow measurements of aerobic microbial growth processes with high biomass productivities. The measuring arrangement makes it possible to describe transitional stages of aerobic yeast cell growth by the ratio of heat production to oxygen consumption (oxy-caloric coefficient). The oxy-caloric coefficient was not constant under the described conditions. The results refer to the existence of an additional energy-delivering mechanism in microbial systems with aerobic carbon source utilization. The mechanism can involve polyphosphate bond division coupled to biomass synthesis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030120
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  • 109
    Electronic Resource
    Electronic Resource
    Masthead (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030201
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  • 110
    Electronic Resource
    Electronic Resource
    Anforderungen der Kultur pflanzlicher Zellen an die Gestaltung der BioreaktorenPoster anläßlich des 2. Symposiums der sozialistischen Länder über Biotechnologie, Leipzig 2.-5. 12. 1980. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 93-95 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030122
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  • 111
    Electronic Resource
    Electronic Resource
    Nachruf für Prof. Dr. Werner Plötner (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 96-96 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030123
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  • 112
    Electronic Resource
    Electronic Resource
    The genus thiobacillus: Physiology and industrial applications (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 99-124 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: This review deals with different aspects concerning the genus Thiobacillus as an obligat chemoautotroph able to oxidize reduced anorganic sulphur compounds. There are given the following topics: physiology of the genus (nutritional requirements, enzymatic complexes for sulphur oxidation, energy generation, reductive CO2 assimilation and growing in presence of organic compounds).The ecological significance of Thiobacilli in the sulphur cycle in Nature is presented. The industrial applications of Thiobacilli in respect to the leaching processes that allow the recovery of metals through oxidation and solubilization from low-grade ores are discussed, with special reference to copper and uranium. A critical revision is done of acid mine drainage impact on the environment, and the use of adequate countermeasures is stressed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030202
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  • 113
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Separation aufgeschlossener Hefesuspensionen (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 133-142 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: By the means of the electronic particle counting, characteristics of industrial contrifuges are found out in order to characterize the performance in the case of the separation of cell debris of mechanically disrupted yeast cells. The knowledge about the particle size distribution complements the usual determination of the dry matter content in the supernatant. Thus an optimization of the separation of tiny particles is facilitated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030204
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  • 114
    Electronic Resource
    Electronic Resource
    Use of biorhythmic processes for increasing the efficiency of carbon-compound conversion by microorganisms (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 125-131 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fermentation kinetic, thermokinetic and morphological investigations of biological carbonsource conversions into microbial biomass and other reaction products can be used for improving the efficiency of biotechnical processes. In this way biorhythmic processes on a macrosocopic scale could be found in transitional stages of microorganism cultivation. These biorhythmic processes can be explained by the occurence of different cell states in the cell cycle. In order to characterize these cell states synchronized microorganism growth was investigated with the aid of the phased culture method. Two states could be observed in the case of yeast growth: the single cell state and the budding cell state. The duration and efficiency of the single cell state is dependent on the growth limitation and the carbon substrate feeding.Thus the reduction of carbon substrate feeding in states of synchronized populations which are characterized by a high percentage of single cells and by energy metabolite production which, in turn, is influenced by carbon substrate concentration can cause increased material and energetic efficiencies of biological carbon-source conversions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030203
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  • 115
    Electronic Resource
    Electronic Resource
    Methanol oxidation and growth yields in methylotrophic bacteria: A reviewSymposium on Biotechnology 1982, Leipzig, G.D.R. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 261-268 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In all bacteria growing on methane or methanol the methanol is oxidised by way of methanol dehydrogenase. This unusual quinoprotein interacts with the electron transport chain at the level of cytochrome c. All methylotrophs having methanol dehydrogenase contain 2 different types of soluble cytochrome c. One of these, but not the other, reacts with methanol dehydrogenase; no intermediate electron carrier is required for this reaction. The P/O ratio for methanol oxidation is 1 although the midpoint redox potential of the methanol/formaldehyde couple suggests that a higher ATP yield might be theoretically possible. Curves are presented that allow the prediction of cell yields, and the effects of altering the system for methanol oxidation, on these cell yields.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030310
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  • 116
    Electronic Resource
    Electronic Resource
    Symposium on the “bioconversion of vegetable raw materials” (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 187-190 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030213
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  • 117
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    Masthead (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030301
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  • 118
    Electronic Resource
    Electronic Resource
    Activity of diaminopimelic acid decarboxylase in bacteria producing lysine (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 217-220 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Activity of DAP-decarboxylase in lysine overproducing strains of Micrococcus luteus, M. varians and Arthrobacter globiformisLb reached the highest level during the end of the exponetial phase of growth and remained at the same high level during the stationary phase of growth when major bulk of lysine was accumulated. In comparison in a lysine non-producing strain of Arthrobacter globiformis I 4 the activity of the same enzyme was low. DAP-decarboxylase of these three lysine overproducers has two specialities, persistence during the stationary phase and insensivity to repression by exogenous lysine.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030303
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  • 119
    Electronic Resource
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    The Leipzig symposiunm on biotechnology 1982 (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 226-226 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030305
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  • 120
    Electronic Resource
    Electronic Resource
    Fermentation. II. Rotenburger Symposium 1980, Bad Karlshafen, September 1980, Herausgegeben von R. M. Lafferty, Springer-Verlag Wien: New York, 1981, 68 M; 31,70 $ (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 58-58 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030114
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  • 121
    Electronic Resource
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    Zur Kinetik der Mischsubstratutilisation bei der Citronensäure-akkumulation durch Saccharomycopsis lipolytica EH 59 (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 143-153 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The mechanism and the kinetic of the assimilation of mixed substrates during the organism growth and the product excretion by strain of Saccharomycopsis lipolytica have been studied. The assimilation of citric acid for the organism growth was prevented when glucose and/or n-paraffins are present as substrates. Citric acid concentrations higher than 30 g/l in the fermentation medium decrease the growth rate on the substrate glucose. Kinetic studies of the mixed substrate assimilation by means of a n-tetradecane-1-614C-labelled paraffin fraction proved that in discontinuous as well as in continuous 1-stage-processes for microbial production of citric acid glucose as substrate is only used for the organism growth whereas the n-paraffin fraction is only used for the acid excretion.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030205
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  • 122
    Electronic Resource
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    Regulation der extracellulären Lipase aus einem alkanverwertenden Stamm von Acinetobacter (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 185-186 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030212
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  • 123
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    Handbuch der Biotechnologie. Wiesbaden, 1982, Akademische Verlagsgesellschaft 634 S., 234 DM (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 191-191 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030214
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  • 124
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    Formal macroapproach to bioprocessing-modeling with analogies (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 195-216 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new approach for the investigations of biotechnical processes is presented in this article on the basis of an integrating strategy, incorporating a joint analysis of biological and engineering aspects. Thereby, the experimental work can be carried out on the macroscopic level, taking into account the behavior of bioprocesses from measurements in the liquid phase. Mathematical modeling uses the concept of analogies instead of speculative mechanisms. A predictive character of these “formalkinetics” supplementary to the descriptive nature is achieved with the aid of deductive research methodology. This general theoretical concept is illustrated in the special case of modeling the diauxic growth of yeast. Formalkinetics based on analogies are shown to be not only able to be successfully applied to elucidate bioprocessing but also to form a more realistic and simple approach than pure mechanistic investigations needed for the realization of bioprocessing in technical scale.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370030302
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  • 125
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    The biochemistry of methylotrophes. Academic Press Inc., London - New York - Paris - San Diego - San Francisco - Sao Piulo - Sidney - Tokyo - Toronto 1982 431 S., 24 £; 49.50 $ (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 192-192 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030215
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  • 126
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    Isolierung von Cytochrom P-450 und des entsprechenden Reductasesystems aus Acinetobacter calcoaceticusBeitrag anläßlich des Leipziger Biotechnologiesymposiums 1982, Leipzig, 21.-25. 9. 1982. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 319-326 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cytochrome P-450 is found in soluble as well as in particulate fractions of n-alkane-induced cells of Acinetobacter calcoaceticus EB 104 as shown by differential centrifugation and; gelfiltration experiments. It is reduced by NADH in the presence of at least two soluble proteins. Particulate cytochrome P-450 was extracted by Triton X-100 and purified to homogeneity. The 80 fold enrichment resulted in a content of 19.0 nmol cytochrome P-450/mg protein. The maxima of the absolute spectra are 417 nm for the oxidized, 408 nm for the reduced and 448 nm for the CO complex of reduced cytochrome. A ferredoxin, identified by maxima of 415 and 460 nm in the absorption spectrum of the oxidized form and by the occurrence of acid-labile sulfur, as well as a corresponding NADH-dependent ferredoxin reductase were isolated independently from the same bacterial strain. Enzymatic reduction of purified cytochrome P-450 is mediated by these proteins, which are discussed as in vivo components of the cytochrome P-450 system of A. calcoaceticus EB 104.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370030404
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  • 127
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    Bioprozeßtechnik. Berechnungsgrundlagen der Reaktionstechnik biokatalytischer Prozesse. Springer-Verlag Wien, New York, 1981 $ 494,-; 198 S (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 338-338 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030406
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  • 128
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    Möglichkeiten der Gewinnung von Koppelprodukten der mikrobiellen Eiweißsynthese auf der Basis von Kohlenwasserstoffen (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 345-350 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The mass production of SCP on the base of hydrocarbons opens the possibility to get a complex of further substances. It is distinguished between by-products - belonging to essential nonprotein - structures of the growing cell and the so - called coupled products accumulating intra - or extracellularly by means of regulation. An important principle of SCP-coupled synthesis of products is the use and the maintenance of proportionality between the growth rate and cell concentration of the producing cells and the accumulation of products. As examples are discussed: fermentation by yeasts and bacteria on the base of n-alkanes, methane and methanol in connection with products as fatty acids, poly-β-hydroxybutyric acid, citric and gluconic acid.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370030408
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  • 129
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    Enzymologie der n-alkanoxidation bei acinetobacterBiotechnologie-Symposium 1982, Leipzig, DDR. (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 251-260 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Enzymology of n-alkane degradation was studied by using different Acinetobactersp¨ Cell-free extracts of A. calcoaceticus69/V oxidize the n-alkane to the corresponding fatty acid via the intermediately formed n-alkanol Soluble and particulate components are necessary for activity. Independently, a rubredoxin and a NADH-dependent rubredoxin reductase were isolated from this strain. Rubredoxin is induced by n-alkanes. The occurence of a cytochrome P-450 was observed only in other strains of Acinetobacter.A. calcoaceticus69/V contains a particulate pyridine nucleotide-independent and a soluble NADP-dependent alcohol dehydrogenase; the particulate aldehyde dehydrogenase is also NADP-dependent. The particulate dehydrogenases are inducible.The results concerning the regulation of enzymes of the citrate and glyoxylate cycle as well as of the malic enzyme indicate that the corresponding fatty acids formed from the alkanes are metabolized via β-oxidation.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370030309
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  • 130
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    New trends in research and utilization of solar energy through biological systems. Edited by H. Myslin und R. Bachofen. Birkhäuser. Verlag Basel-Boston-Stuttgart 1982, 156 S (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 278-278 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030312
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  • 131
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    Einfluß von Kupfer-, Mangan- und Zinkionen auf die makromolekulare Zusammensetzung der Hefe Candida utilis M 72 (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 357-362 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ions of Cu2+, Zn2+ and Mn2+ affect significantly the macromolecular composition of yeast Candida uiilis M 72. Crude protein and nucleic acid content increase and carbohydrate content decreases with rising intracellular ion concentration. Lipid fraction is influenced by metallic ions only little.The correlation between intracellular ion concentration and cell composition of macromolecules can be described by simple equations.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370030410
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  • 132
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    Masthead (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030101
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  • 133
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    Traditional food fermentations as industrial resources (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 3-12 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Asia is rich in resources which can contribute widely to food processing and production over the next 207-50 years as world population reaches 6 billion. The world needs low cost methods of providing nutritious proteinrich meat analogues for its thousands of millions of consumers. The Indonesian tempe fermentation will serve as a model. A bacterium present in commercial tempe can be used to add vitamin B-12 to other vegetarian foods. Fuel requirements for cooking can be decreased by applying a fungal fermentation of the tempe/ontjom type to legume substrates. The world needs high quality meat-flavors derived from vegetable protein. The soy sauce (kecap)/miso (tauco) processes and the fish/shrimp sauce and paste processes can be modified to yield a wide variety of meat-like flavors for use in formulating new foods. The protein content of high starch substrates can be increased by applying the Indonesian tape fermentation. Leavened sour-dough bread-like products can be produced without the use of wheat or ryeflours using the Indian idli/dosai fermentation. Coconut protein, if extracted without denaturing, can serve as a valuable base for a new type of puddings and related foods. Finally Asia is an almost endless source of cultures of edible microorganisms that, with further study of their synthetic abilities, particularly regarding amino acids and vitamins, could lead to new industries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030102
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  • 134
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    Factors affecting growth and cellulose hydrolysis by the thermotolerant aspergillus nidulans from composts (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 65-72 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability of Aspergillus nidulans (EIDAM) WINT to grow and sporulate at various temperatures and to degrade soluble and insoluble forms of cellulose were studied. A. nidulans was found to grow and sporulate best at 37°C in continuous light and alternating light-darkness respectively. The fungus was able to cause losses in the dry weights of filter papers on incubation and made appreciable growth on CMC and hemicellulose. The culture filtrates contained cellulases which hydrolysed filter papers and CMC to reducing sugars, and were only able to produce these enzymes in the presence of cellulose or its derivatives in the growth medium. The CM-cellulases had peak activity at pH 5.2 and at 50°C while optimal FP-activity occurred at a pH of 5.5 and at 45°C. The participatory role of A. nidulans in composting is discussed.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370030116
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  • 135
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    Die Bedeutung des Erhaltungsstoffwechsels und der „maximalen“ Ausbeute (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 73-75 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper examines the microbial denitrification of a medium containing acetate as C-source and of an industrial effluent containing volatile fatty acids as main components a ndphenolics as minor components.Values for m and Ymax are represented. It has been concluded that the maintenance coefficient comprises also those processes not involved in the maintenance metabolism of the cells.The least fraction of the rate of substrate utilization of this extended maintenance metabolism has been expressed by m · 100/qmax. From the values of Ymax a P/2e-ratio 〈 1 and the equation of cell synthesis have been evaluated.
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/abio.370030117
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  • 136
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    „Biotechnology of microbial synthesis“. Riga 1980, Sinatne publishing house, 350 pp, 74 fig., 59 tab., 582 ref. (Russian) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 42-42 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020103
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  • 137
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    Russian Article pp. 51-58 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 51-58 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper considers techniques of measurement of the curves of oxygen consumption by microorganisms. The widely applied method of obtaining the value of the critical oxygen concentration (COC) using these curves has been analysed. The experimental conditions necessary for the adequate measurement of the culture respiration rate in a fermenter have been found. It has been shown that in the case when the respiration rate within the considered range of pO2 is determined by one and the same enzyme, the COC value is not an apropriate characteristic of the mode of the respiration rate dependence on oxygen concentration.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020105
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  • 138
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    Patents reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 192-192 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020214
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  • 139
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    Immobilization of liver microsomal cytochrome P-450 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 187-191 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Rabbit liver microsomal cytochrome P-450 was immobilized by entrapment in calcium alginate gel. Aminopyrine demethylation experiments showed that the immobilized enzyme system is highly active and exhibits an unimpaired functional stability as compared with crude microsomes. The alginate entrapped microsomes were employed in a fixed bed recirculation reactor, where aminopyrine was continuously demethylated. Such model enzyme reactor can be a useful tool for studying extracorporeal drug detoxification or preparative substrate conversion with microsomal enzyme systems.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020213
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  • 140
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020301
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  • 141
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    Das Autoselect-System - ein Automatensystem zur Selektion von Antibiotikaproduzenten, VII. Hemmhofmeßautomat (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 193-198 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The zone reader of the Autoselect-system enables to measure the 64 inhibition zones of a quadratic bioassay plate with an accuracy of 0,1 mm within 3 minutes. A carriage moves each zone in the light beam. With a special photometrical device 3 defined areas of the inhibition zone can be measured quantitatively by 2 receivers. The indicated values of the diameters result from the analog treatment of the signals. By coupling to a computer the datas of the measuring and other desired informations are printed out.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020215
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  • 142
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    Das Autoselect-System - ein Automatensystem zur Selektion von Antibiotikaproduzenten VIII. Zur Effektivität des Automatensystems (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 199-204 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The advantages of the Autoselect-system are explained. They are offered by a higher speed of all manipulations, by a significantly better accuracy, by getting more informations and by easier physical labour. In the most cases the automated work can be done in a fourth till a tenth of the time needed for manual handlings. The capacity of the machines amounts 5 000 to 30 000 colonies/samples per day. Special problems as well as possibilities for a further increase of the efficiency are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020216
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  • 143
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    Man-made microbes and man-made lawBased on a lecture given at a seminar for representatives of the Academy of Finland and the Academy of Sciences of the G. D. R. in Jena, Dec. 1980. (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 207-212 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The Supreme Court of the US in 1980 granted a patent for a strain of Pseudomonas containing two plasmides after genetic manipulation. This is the first case of patenting a living organism. Whereas the patent law of US and of many other countries further more on supports the principle that natural products are not patentable man-made microorganisms on the other hand fullfil very important crucials of patentability as far as they are new, unobvious, reproducible and useful. The situation arising as the result of this decision is described and the implications and consequenses for the patent law, the taxonomy and the general biological thought are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020302
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  • 144
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    Immobilized enzymes for food processing. Boca Raton, Florida; 1980; CRC Press, Inc. 220 Seiten; 73.95 $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 226-226 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020304
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  • 145
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    Russian Article pp. 213-225 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 213-225 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of assimilation of hydrocarbons having a different structure by Candida yeasts was studied.The rates of oxidation of various carbon atoms in the molecules of isoalkanes, alkylaromatic hydrocarbons and n-alkanes were evaluated in the range of C11 to C28.Metabolic inhomogeneity of carbon atoms in the molecules of isolakanes and alkylaromatic hydrocarbons was observed. A competition in the assimilation of the called hydrocarbons and n-alkanes and also a competition in the assimilation of n-alkanes of a different molecular weight, i.e. metabolic inhomogeneity of carbon atoms of n-dodecane and n-tetracosane was found out for yeasts.
    Additional Material: 11 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020303
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  • 146
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    Modeling step function responses in continuous fermentation processesPaper presented, in part, at the Second Chemical Congress of the North American Continent/180th National Meeting of the American Chemical Society, Division of Microbial and Biochemical Technology, Las Vegas, Nevada, August 28, 1980. (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 227-238 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Understanding both the qualitative and quantitative transient responses to inlet flow and substrate step functions has been a relatively unsolved problem for continuous fermentation processes. This study of transient responses and simple descriptive models suggests that the saturation constant of the MONOD equation is a variable. Thatf variable depends on the rate constants of parallel biochemical pathways leading to cell growth, the concentration of cells themselves, and the concentration of all products in the culture. Mechanisms for describing the hysteresis behavior of the growth rate funcion are postulated in terms of the underlying molecular biology of the microbial system.
    Additional Material: 13 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020305
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  • 147
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    Ein mikrorechnersystem für fermentationseinrichtungen (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 239-249 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For several years now, we can notice efforts in increasing the efficiency of microbial processes by means of more intensive scientific investigation of such processes. Essential prerequisites to it are improved possibilities for process monitoring (available sensors) as well as facilities for realtime processing of process information. In this paper a microcomputer system is represented, which has been constructed on the base of computing needs for fermentation. The computing needs for fermentation experiments are outlined and the structure of the microcomputer is described. As an example it is illustrated what tasks can be solved by this microcomputer in coupled operation with a laboratory fermentor.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020306
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  • 148
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    Lecture Notes in Biomathematics, Vol. 41., Modèles Mathèmatiques en Biologie, Springer-Verlag, Berlin/Heidelberg/New York, 1981, 219 Seiten, 29 Abb., 28, 50 DM; 13.00 US $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 250-250 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020307
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  • 149
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    Rechnergestützte führung von fermentationsprozessen Teil I (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 251-262 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The industrial continuous fermentation for the production of microbial protein is like any real process subject to disturbances. The considerable social expenditures involved in production make it necessary to restrict the negative consequences of these disturbances to a minimum by means of suitable measures. One such measure is the computer-aided adaptation of the static optimum. For the reaction on nonmeasurable disturbing inputs an algorithm is given containing the steps data filtering, adaption of process model and optimization, and the solution of the data-filtering problem in the widest sense by spline functions is discussed. The application of this algorithm to a specific problem of process control is demonstrated in [1].
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020308
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  • 150
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    Beitrag zur Optimierung des mechanischen Aufschlusses von Hefezellen (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 263-274 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of technological variables on the cell disintegration of yeast suspensions by means of a ball mill and a high pressure homogenizer has been studied by measuring the electrical conductivity. The rate constants and half-life periods are calculated. Regarding the production of protein isolates, the stipulation of optimal disintegration conditions requires a compromise between a degree of disruption as high as possible and a low destruction of the cell walls.By homogenizing, fragments of cell walls arise which are more uniform and better separable in comparison with the milling process. Therefore the mechanical breakage of yeast cells on a large scale should be carried out by the use of high pressure homogenizers.
    Additional Material: 7 Ill.
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  • 151
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    Wasseraufnahme und enzymfreisetzung im getreidekorn (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 297-298 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020312
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  • 152
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    Untersuchungen zum Anfangsstadium der enzymatischen Hydrolyse unterschiedlich vorbehandelter Linterscellulose (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 275-286 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Starting from cotton linters cellulose modified in physical structure type of lattice, degree of order, state of swelling by different pretreatments, and from culture filtrates of Gliocladium spec., the initial stage of enzymatic hydrolysis of cellulose has been investigated. Especially with substrates of high degradability a considerable effect of stirring on rate of formation of soluble products was found. For linerization of yield-vs-time-curves (% residue resp. 7percnt; solubles as a criterion for yield), a 2-parameatric first order rate law was found to be suitable within a limited time interval, values of k1 were higher and for the accessible part of the substrate were lower in the initial stage of hydrolysis than in the later one. The MICHAELIS-MENTEN-constant kM has been determined for substrates of different physical structure after different times of reaction. Data found for kM indicated a stronger dependence of kM on reaction time than on physical structure of the substrate under conditions applied.
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  • 153
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    Bestimmung von Substratverbrauchskoeffizienten für Paraffinkohlenwasserstoffe (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 299-302 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The empirical determined constant of 3.14 gram biomass per available electron is a good base for the calculation of minimum substrate consumption coefficients in the aerobic fermentation of paraffins by yeasts.The analysis of experimental determined specific substrate consumption coefficients and their comparison with the corresponding theoretical values show that the theoretical ones can be reached only if the substrate composition referring to carbon and hydrogen is of optimal composition for all syntheses.
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    URL: http://dx.doi.org/10.1002/abio.370020313
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  • 154
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020401
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  • 155
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    „Ernährungslehre und diätetikldquo;, bd. I, Teil 1 und 2. GEORG THIEME Verlag Stuttgart-New York 1980, 625 Seiten, Teil 1 - 40 Abb., 42 Tab., Teil 2 - 148 Abb., 90 Tab., Preis jeweils 248,- DM (Summe 496 DM), diverse Lit,-Zit. (kapitelweise) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 303-303 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020314
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  • 156
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    Biotechnology. Verlag Chemie, Weinheim; Deerfield Beach, Florida; Basel, 1981, Volume 1: Microbial Fundamentals, ca. 550 Pages, 247 Figures, 106 Tables, DM 495,-; Subcriptionprice DM 425,- (if taking all volumes), Time for subcription up to June, 30, 1982 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 304-304 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020315
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  • 157
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    Biotin vitamers content of lactose and beet molasses (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 369-375 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The biotin activity of beet and lactose molasses against the test strain Saccharomyces cerevisiae 225 by auxanographic method was evaluated. The level of lactose molasses biotin activity is almost twice as high as that obtained in the case of beet molasses. The results of bioautography with test strains Saccharomyces cerevisiae 225 and Lactobacillus arabinosus 17-5 indicate the qualitative composition of biotin derivatives (vitamers) in both molasses. Depending on the various technological steps e.g. sterilization or clarification one may find differences in the content and qualitative composition of biotin vitamers.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020409
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  • 158
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020101
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  • 159
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    Status and developments of animal cell technology using suspension culture techniques (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 3-41 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In this review the state of the art in animal cell technology, using suspension culture techniques is updated as far as the end of 1980. We have tried to discuss, on a broad basis, the current status and potential developments of both, the purely biological and the biochemical engineering aspects which may be important to improve the performance and design of animal cell technologies. The process economics could be considerably improved by the use of transformed animal cell substrates, the use of cheaper cultivation media and by methodological and engineering means. Most of these aspects are in the state of realization. Nevertheless, for a great variety of biological active substances which do not require so - or posttranslational processing, recombinant DNA-techniques (e.g. genetic engineering) are a promising alternative for the production of animal cell derived substances.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020102
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  • 160
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    Enzyme engineering, vol. 5. New York-London, 1980, Plenum Press, 491 Seiten; 49.50 $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 72-72 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020107
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  • 161
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    Grundlagen der Verfahrenstechnik. Springer-Verlag Wien/New York 1981 377 Seiten 138 DM (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 78-78 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020109
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  • 162
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    Die Anwendung von Produkten der mikrobiologischen Synthese in der Tierproduktion. Verlag „Kolos“, Moskau, 1980, 288 Seiten, 112 Tabellen und Übersichten (in russischer Sprache) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 86-86 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020111
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  • 163
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    Gewinnung und Charakterisierung von Prolidase aus, Escherichia coli B., I. Gewinnung des Enzyms (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 87-94 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: An enzyme being able to hydrolize the imido linkage at the N-terminal end of proline is isolated from E. coli B. This fact corresponds to the specifity of hydrolization of the animal prolidase. Enzyme synthesis within the cells of E. coli B is carried out independently from growth. Changed environmental factors may influence the formation rate of enzyme in a restricted way. A relatively high enzyme biosynthesis can be reached by cultivating the strain E. coli B at a temperature of 37°C as well as an initial pH-value of the medium of 7.0 in submerged culture (400-500 rpm) By variation of the medium composition enzyme synthesis does not change considerably, however, biomass yield can be increased about 100% If mechanical cell desintegration is optimized by means of ultrasonic or vibration homogenisator the cell components may be easily released with a higher proline specific activity as animal prolidase.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020112
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  • 164
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 114-114 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020116
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  • 165
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 115-115 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020117
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  • 166
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 116-116 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020118
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  • 167
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    Regulation der mikrobiellen Alkanoxidation mit Hinblick auf die ProduktbildungVortrag, gehalten anläßlich des 2. Symposiums der sozialistischen Länder über Biotechnologie, Leipzig, 2.-5. 12. 1980 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 127-138 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Catabolic pathways of long-chain n-alkanes in the range of C8 to C18 are demonstrated and results of investigation about the regulation of monoterminal oxidation are given: - Enzymes of monoterminal alkane oxidation usually are inducible- Several intermediates of alkane oxidation can inhibit the primary oxidation of concerned alkane-Substances, e.g. glucose and glycerol, which ordinary don't be developed in catabolic alkane reactions, in many cases have an inhibitory effect on alkane oxidationThe regulation of catabolic pathways has a great influence on the formation of specific products This influence is demonstrated examplarily at the production of biotin, fatty acids and citric acid.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020203
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  • 168
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    Practical aspects of the mathematical modeling of fermentation processes as a method of description, simulation, identification and optimizationPaper given at the 2nd Symposium of Socialist Countries on Biotechnology, Leipzig 2.-5. 12. 1980 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 119-126 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Most mathematical models for describing the physiological state in fermentations lead to solutions of the so-called “stiff differential systems” during simulation on a digital computerThere is no suitable conventional software for solving these systemsAs a result of a relatively extensive screening of suitable methods for the solution of “stiff” differential systems (about 200 methods) it may be concluded that the semiimplicit RUNGE-KUTTA-formulas of the ROSENBROCK type, which constitute a part of the collection of programmes STIFFSOLVER-80, are optimal for the simulation of fermentation processesFor determining kinetic parameters from integral data the authors use the system of programmes BIOKINTheir practical application is discussed for 3 examples: 1Growth of the yeast Saccharomyces cerevisiae and changes in the content of specific compounds (proteins and ergosterol)2Quantitative evaluation of “;direct oxygen transfer” in the submerged culture.3Biosynthesis of a new antibiotic substance mucidin.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020202
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  • 169
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    The blue light syndrome. Springer-Verlag, Berlin-Heidelberg-New York (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 178-178 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020210
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  • 170
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    Immobilization of β-glucosidase on an inorganic carrier (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 179-185 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The enzymatic hydrolysis of cellobiose, an important intermediate of the decomposition of cellulose containing materials, with immobilized β-glucosidase preparations from Geotrichium candidum, Trichoderma lignorum and Aspergillus foetidus was examinedAt first it was the aim to prepare from differently purified samples with different specific cellobiase activities high active preparations on the basis of the inorganic carrier Silochrom S-80. Characteristics e.g. thermal stability and temperature and pH optimum of immobilized preparations were compared with those of soluble preparationsKinetics of cellobiose hydrolysis by immobilized enzyme preparations were studied.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020211
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  • 171
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    Der Einfluß der Flockenbildung auf die Versorgung der Hefezellen mit Sauerstoff bei der Fermentation flüssiger Kohlenwasserstoffe (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 43-50 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Floc formation, especially the influence of floe diameter variations on the total velocity of the process, was investigated in aerobic growth processes of yeast on the hydrocarbons of crude oil.The experimental results show that the diameter of the flocs is a function of the rheological properties of the fluids and the flow conditions.The floc diameter varies between 0,1 mm and a few millimeters. About 90% of the total yeast cells are situated in the interior of the flocs.Since oxygen must be transferred to all yeast cells their oxygen supply was studied.Thus, the yeast cells in the floc interior were not sufficiently supplied with oxygen, if the floc diameter reached a critical value.In such cases a decrease of the biomass formation rate was observed, although the dissolved oxygen concentration of the aquaeous fermentation medium was greater than zero. Therefore, aerobic microbial growth processes in multicomponent systems must be carried out without floc formation or under such conditions as cause very small floc diameters.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020104
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  • 172
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    Some aspects of fermentation as an unit process in chemical engineering (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 59-71 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are described possibilities of scale up in stirred tank fermenters being based on known relations of mass-, energy-and heat transfer. The represented mathematical description shows the problems of the necessary scale up in the practice with the theory of similarity in the stirred tank fermenter. Relating on the method of RUSHTON for calculation of the energy capacity in different stirred fermenters the influence of the aeration is examined and confirmed with experimental results of the author. By the sysem of equations the known parameters of the aerobic fermenters can be calculated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020106
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  • 173
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    Effektivität von Modellierung und Optimierung mikrobieller Prozesse (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 73-77 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aim of the technological treatment of microbial processes is the optimal working of the production plant. The treatment itself is to be seen as a process whose realization requires a certain amount of expenditure. The problem of optimization of this process can with certain restictions be approximately solved in a series of steps. After a general formulation of the problem explanations are given for two important and typical steps - the determination of the extend of modelling and the choice of production cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020108
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  • 174
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    Regulation of lysine biosynthesis in Brevibacterium flavum (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 79-85 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: L-lysine synthesis pathway enzyme activities: β-aspartate kinase (EC.2.7.2.4), diaminopimelate decarboxylase (EC.4.1.1.20) for two L-lysine producing strains Brevibacterium flavum 22LD and RC-115 were studied. It has been found that β-aspartate kinase and diaminopimelate decarboxylase in the Br. flavum RC-115 are less sensitive to feed-back inhibition by lysine and threonine. It is supposed that desensitized β-aspartate kinase in the Br. flavum RC-115 can be determined by genetical changes of the regulatory properties of the β-aspartate kinase.Auxotrophity in the locus of homoserine dehydrogenase was tested and no homoserine dehydrogenase (EC.1.1.1.3) activity was found in either strain.The combination of these both types of mutation supplemented by the lack of catabolic repression in the RC-115 strain makes it an active lysine producer in the medium with high carbohydrates content.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020110
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  • 175
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    Erzeugung eines zellwandlytischen Enzymkomplexes aus Arthrobacter GJM-I zur Herstellung von Protoplasten aus Candia spec. H (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 95-102 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growing conditions have been found out for the bacterium Arthrobacter GJM-I to produce a lytic enzyme system, which converts cells of the yeast Candida spec. H to protoplasts quickly and in a good yield. Estimating the activities of α-mannanase and β-glucanase we found out the optimal culture time to gain the lytic enzyme system from the culture filtrate. It was shown that radioactive labeling of the yeast cells makes it possible to estimate quantitatively the conversion to protoplasts and the simultaneous lysis. The obtained lytic enzyme system can substitute the snail cnzyme system which was used for cell conversion of Candida spec. H to protoplasts till now.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020113
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  • 176
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    Calorimetric Glucose Determination by Glucose Oxidase  - Methylene Blue (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 103-106 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The reaction between glucose and methylene blue, catalyzed by glucose oxidase (GOD)was analysed calorimetrically.The amount of heat produced under saturating methylene blue concentrations ( 〉 10-2 mol/1)was measured with glucose concentration and time as parameters (kinetic procedure) Kinetic constants (pseudo one substrate kinetics) were derived from the experimental data: KM(glucose)= 1.18 × 10-3 mol/l and Vmax = 0.085 J/mg GOD min (3.89 · 10-6 mol/mg GOD min)Comparison of caloric with optical measurements gave an enthalpy of reaction of 22.52 kJ/mol. Considering the observed substrate inhibition, glucose determinations are possible up to glucose concentrations of 0.1 mol/l.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020114
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  • 177
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    Ethanol from Zymomonas, a bacterium (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 307-315 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Thirteen isolates ofZymomonas were analyzed for their ability to tolerate increasing concentrations of glucose and ethanol. In medium containing 5.0% (v/v) ethanol, four isolates grew well in 15.0% (w/v) glucose. Six cultures tolerated at least 6,0% ethanol. Of all the isolates, 7 preferred glucose and 4 preferred sucrose as a sugar substrate. In a nutrient medium containing mineral salts and high concentrations of pantothenate and biotin ethanol production for 2 isolates was approximately 7.0%. Continuous stirring and growth factors were responsible for this increased ethanol production.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020402
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  • 178
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    Einfluß der limitation des wachstums von hefen auf den oxidativen stoffwechsel und die produktsyntheseVortrag gehalten anläßlich des 2. Symposiums der sozialistischen Länder über Biotechnologie, Leipzig 2.-5. 12. 1980 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 317-324 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The growth limitation of aerobic yeast cultures in the majority of cases involves a deep conversion of the metabolism and high changes in the functional state of the cellsThe excretion of metabolits as one of this changes of great importance and the other variations in the state of the cells are the consequence of functional alternations of several anabolic and catabolic processes.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020403
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  • 179
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    Entwicklung von Berechnungsmodellen für Enzymreaktoren (Teil 1) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 325-330 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: It has been developed a model for a continuous working enzyme-reactor to determine the deviation of temperature and concentration in axial and radial direction. It's based on Partial Differential Equations for mass and enthalpy transfer. The model regards the specialities of kinetics of enzyme-catalytic processes with respect to the reaction rate. A difference method had been choosen which provided applicable results with respect to characteristic quantities of the substance under corresponding initial and boundary conditions. Some pictures show the deviation of temperature and concentration.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020404
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  • 180
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    A desk-top computer coupled fermentation system for the study of microbial processes: Description and some applications (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 331-336 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The configuration and hardware components of a desk-top computer coupled system for bench-top bioreactors are described. Examples of on-line acquisition of several directly accessible environmental process parameters and computations of directly inaccessible state variables are presented. The system described offers great advantages in experiments for establishing sophisticated control algorithms and for studying the physiological behaviour of microbial populations.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020405
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  • 181
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    Extrazelluläre Lipase aus Acinetobacter calcoaceticus (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 337-342 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cell-free growth liquor of Acinetobacter calcoaceticus 69-V contains an extracellular lipase. Its activity depends on growth phase and carbon source. During growth on acetate or succinate the activity ist low or zero, respectively. Growth on alkanes causes an increase in the extracellular lipase activity. Activity reaches maximum values during the exponential phase of growth which significantly decrease in the stationary phaseDuring the growth on alkanes some surfactants (Tauroglycocholate, Triton X-405) stimulate the excretion of the enzyme and some other (Tween, Brij, Triton X-100) inhibit the lipase and growth of cells, respectivelyAir-water and alkane-water interfaces inhibit the lipase activity. During starvation of the bacteria grown on alkanes lipase is excreted in the starvation medium.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020406
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  • 182
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    Einfluß von Penicillin und seinen Zersetzungsprodukten auf die Biosynthese von L-Lysin mit Brevibacterium flavum CBPaper given on 2nd Symposium of Socialist Countries about Biotechnology, Leipzig 2.-5.12.80 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 107-113 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the laboratory scale fermentation the substitution of peanutmeal by a hydrolysate of Penicillium mycelium in the culture medium for production of L-Lysine with Brevibacterium flavum CB has been testet. The mycelium hydrolysate contains Penicillin and degradation products of Penicillin; therfor the influence of these substances to production of L-Lysine has been investigated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020115
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  • 183
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020201
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  • 184
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    Entwicklung und Anwendung einer Methode zur Bestimmung der spezifischen Oberfläche von HefetrockensubstanzBeitrag anläßlich des 2. Symposiums der sozialistischen Länder über Biotechnologie, Leipzig, 2.-5. 12. 1980 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 139-144 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The knowledge of surface area of microbial biomasses is necessary in connection to mass-transfer processes for instance extraction process. The method of BRUNAUER, EMMETT and TELLER (BET) based on adsorption processes was adapted to determination of surface area of samples of dry yeast. Comparative investigations and error estimations show the possibility of application and the evidence of this method.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020204
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  • 185
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    Elektronenmikroskopische laborpraxis. Eine methodensammlung mit bildbeispielen für lehre und forschung in der Medizin und Zellbiologie. Springer-verlag, Berlin7ndash;Heidelberg-New York, 1981 38,-DM; 18,10 $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 154-154 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020206
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  • 186
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    Gewinnung und Charakterisierung von Prolidase aus Escherichia coli B, II. Charakterisierung, Reinigung und Trägerfixierung des Enzyms (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 161-170 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Prolidase, a specific exopeptidase, is isolated from Escherichia coli B. The enzyme being present in the raw extract is purified and enriched by fractionated ammonium sulfate precipitation, ion exchange chromatography on DEAE-Sephadex A 50 as well as by gel filtration on Sepharose 4 B. Total yield of prolidase amounts to 19% with a 67fold enrichmentSubstrate specifity of the enzyme mainly corresponds to that of the animal prolidase. It is able to hydrolize the imido linkage at the N-terminal end of prolin in the case of di- and tripeptides. The temperature optimum of prolidase from E. coli B is 37 °C, the pH-optimum from pH 7.6 to 9.0. Storage stability at pH 8.6 and a temperature of 4 °C is optimal. The enzyme is only active in presence of Mn2+-ions. This metal cannot be replaced by Mg2-- or Zn2+-ionsA high enzyme activity and storage stability in presence of Mn2+-ions can be reached by immobilization of the prolidase, by covalent binding on Sepharose 6 B, adsorption on DEAE-Sephadex as well as by combination with glutar dialdehyde on DEAE-Sephadex.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020208
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  • 187
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    Zur Kinetik der Nebenproduktbildung bei der Überproduktion von Citronensäure durch Saccharomycopsis lipolytica (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 171-177 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: After exhaustion of the N-sources the yeast S.l. excretes citric and isocitric acid with high rates without interferring in the postlogarithmic phase the intracellular production of reserve materials like polysaccharides and especially lipids. The synthesis of citric acids and of reserve materials are therefore autonomically proceeding processes inside of the cellsWith increasing lipid content the ergosterol content increasesThe utilization of the ergosterol rich yeasts as valuable byproduct of the citric acid production is discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020209
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  • 188
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 186-186 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020212
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  • 189
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    RNA removal from Candida utilis by chemical and thermal treatments (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 145-153 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The removal of the content of nucleic acids of fodder yeast (Candida utilis) by treatment with HCl or heat shock was investigatedAcid concentrations between 5 and 15% (on dried matter basis) were used. A maximal removal of the content of nucleic acids of 88% was realized wheńn was worked with 15% HCl, 90 °C during 30 minutes. But under this conditions were observed high demanges of protein and also of some essential amino acidsGood results for diminishing the content of nucleic acids were reached with the highest concentration of acid and a treatment during 20 minutesThe experiments with heat shock were carried out at 68 °C, different times for heating and different contents of yeastIn this way better results than for treatment with acid according to diminishing the content of nucleic acids and yield of protein and essential amino acids were reachedA removal of over 80% of the content of nucleic acids was achieved in all cases.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020205
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  • 190
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    Untersuchungen zur chemischen Zusammensetzung der Lipidfraktion von Acinetobacter calcoaceticus (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 155-160 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lipids were extracted from the cells of Acinetobacter calcoaceticus EB 10 C IMET B 395 grown on gas oil (Bp. 240-360 °C) with benzine/alcohol (80 : 20). The lipid-hydrocarbon-extract obtained by this extraction method was 18.4%. The extract was composed of hydrocarbons, waxes, phospholipids, fatty acids, glycerides, and ubiquinones. The main components among the lipids were waxes. The compositions of phospholipid, fatty acid, wax, and ubiquinone fractions were analysed.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020207
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  • 191
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    Lysine production from hydrocarbon by micrococcus species (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 287-296 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A bacterium isolated from Assam (India) soil was found to accumulate L-lysine in the mineral salt-hydrocarbon medium and identified to be a strain of Micrococcus luteus. The strain is able to grow and accumulate l-lysine in a purely synthetic medium, but supplementation of the synthetic medium with casamino acid or yeast extract or both, improves the yield. The entire fermentation period can be divided into a growth phase and a production phase, which can be prolonged by adjustment of the pH to the neutral range. Among the different hydrocarbon and nitrogen sources tested straight run gas oil (47percnt;) and ammonium sulphate (0.4%), respectively, were found most suitable.Erythromycin at 1 μg/ml level inhibited growth bu¸t stimulated lysine excretion. An inoculum level of 10% (v/v) of the medium was optimal for lysine production. The yield of lysine under optimal conditions was found to be 3.25 g per litre of the medium. Lysine has been isolated in crystalline form from the fermented broth by ion exchange resin chromatography and found to be pure sample of L-isomer.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020311
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  • 192
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    Immobilisierung mikrobieller Zellen und deren Nutzung zur Substratwandlung - Eine Literaturstudie. 1. Fortsetzung (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 343-358 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The first supplement of a review on recent literature is given concerning the immobilization of whole microbial cells and their testing for application in product synthesis. It includes the same topics as the first review, essentially, together with the table of the immobilized microbes and the tested reactions of product synthesis or analytical purpose, respectively, with 177 references.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020407
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  • 193
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    Biosynthesis of polyribonucleotide phosphorylase and polyribonucleotides by E. Coli (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 359-368 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The biochemical functions of Polyribonucleotide phosphorylase (PNP-ase; EC 2.7.7.8) has been studiedThe present work was aimed at studying the interrelation between PNP-ase biosynthesis and RNA content of E. coli cells under various conditions of bacterial growth, obtaining the biomass of E. coli with high PNP-ase activity as well as to study the possibility of secondary cultivation of E. coli cells for obtaining polyribonucleotides from nucleoside-5′-diphosphatesThe spcific PNP-ase activity increases at secondary cultivation, when the medium contains ribonucleoside-5′-diphosphates. Besides one may observe an extracellular polycondensation of nucleoside-5′-diphosphatesIt may be presumed that the PNP-ase is a multifunctional enzyme whose biological role may be confined to regulating the energetic processes connected with the metabolism of glucose, nucleosidephosphates and orthophosphate in the cells of E. coli.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020408
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  • 194
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    Bioprozeßtechnik - berechnungsgrundlagen der Reaktionstechnik biokatalytischer Prozesse. Springer Verlag, Wien/New York, 1981 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 387-387 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020411
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  • 195
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    Die chemische Hydrolyse von Reisschalen und das Wachstum einer Candida tropicalis-Kultur auf den Reaktionsprodukten (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 377-385 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the prehydrolysis of rice hulls where investigated with different concentrations of H2SO4 and temperatures by modified ARRHENIUS-equation in relation to the degradation of pentosans and the formation of reducing sugars. The determination of the activation energy, the frequency-factor and the orden of reaction in relation to the concentration of the acid results for the formation of the reducing sugars in 60.3 kJ/mol, 5.28 ×. 107 and 1.76, for the degradation of the pentosans in 59- and 69.9 kJ/mol, 3.08 × 107 and 6.03 times; 107 as well as 0.76 and 0.85The discontinuous growth of the yeast Candida tropicalis QML 7601, isolated from a biotop in Cuba, on the products of the prehydrolysis of the rice hulls with H2SO4 (0.1- and 0.2 N) and different mixtures of H2SO4 and HNO3 yield values of μmax = 0.3 - 0.6 h-1 in relation to the conditiones.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020410
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  • 196
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    Electronic Resource
    Bioconversion of cellulose wastes to protein and sugarPaper given at the 2nd Symposium of Socialist Countries on Biotechnology, Leipzig 2.-5. 12. 1980 (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 365-370 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A schematic representation of the variety of products which can be obtained by microbial conversion of cellulose is presented.Alkaline pre-treatment has been used after milling in all the experiments. Solka-floc or sugarcane bagasse was used as sources of cellulose. A cellulolytic strain of Aspergillus terreus (ATCC 30514) was cultivated in batch-, fed batch and continuous culture up to 7 liter stirred tank fermenter. The general growth characteristics were determined by growing on glucose. Results of experiments on the growth of A. terreus for production of biomass on Solka-floc or Sugarcane bagasse are given, also the ability of crude cellulases to produce sugar syrups by enzymatic hydrolysis of cellulose has been evaluated.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010408
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  • 197
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    The renaissance of biotechnology: Man, microbe, biomass and industry (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 207-246 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010302
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  • 198
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    Untersuchungen zur Mischung in einem 500 m3-Rührfermentor-System bei der mikrobiellen Biomasse-Synthese unter Einsatz einer Erdöl-destillat-Fraktion (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 247-267 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Examination show how far the stirred tank fermenter meets the requirements of multi-phase systems under conditions of microbiological protein synthesis. 1The homogeneity attained by mixing of 4-phase-fermentation medium will be influenced by different amounts of material flow of the correspondent process conditions.2Limitation of nitrogen and solved nutrient - and trace salts in culture liquid by unsatisfactorily mixing can be excluded. The solute-oxygen concentration and homogeneous distribution of solute-oxygen in the culture liquid are influencing the economy of fermentation.3The homogeneity concerning petroleum distillate-, biomass-and air distribution highly depends from biomass content of the fermentation medium.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010303
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  • 199
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    Untersuchungen zur chemischen Zusammensetzung der Lipidfraktion von Lodderomyces elongisporus EH 15 (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 279-284 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lipids were extracted from the cells of Lodderomyces elongisporus EH 15 grown on gas oil (Bp. 240 to 360 °C) with benzine/alcohol (80:20). The lipid-hydrocarbon-fraction obtained by this extraction method was 18.5%. It was composed of hydrocarbons, phospholipids, fatty acids, glycerides, sterols, ubiquinones, and vitamines. The main components among the lipids were phospholipids. The compositions of phospholipid, fatty acid, sterol, and ubiquinone fractions were analysed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010306
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  • 200
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    Die Technologie von mikrobiellen Eiweißpräparaten, Aminosäuren und Fetten. Verlag Lebensmittelindustrie, Moskau, 1980. 448 Seiten, 119 Abbildungen, 55 Tabellen, 32 Empfehlungen auf sowjetische Fachliteratur (in russischer Sprache) (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 376-376 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010410
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