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  • 101
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 35-40 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Long-term continuous ethanol production of up to 80 g.l1 with a volumetric ethanol productivity of 63 g. l-1. h-1 was maintained for more than 72 days using a Vertical Rotating Immobilized Cell Reactor of the bacterium Z. mobilis. Continuous production of higher ethanol concentration was unsuccessful due to an inhibition of cell growth by long exposure to high ethanol concentrations. However, ethanol concentration as high as 120g. l-1 and volumetric ethanol productivity of 13g. l-1. h-1 were achieved in a repeated-batch fermentation system using the same bioreactor. By a simple washing operation at the end of each run, immobilized biomass could be effectively regenerated and used to carry out more than 10 successive fermentation cycles.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 102
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 23-34 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are many points of contact between optimization problems and modeling. On the one hand the model adjustment process itself as a process of estimation is closely connected with optimization, in that it is to produce what is in one sense the best possible model. The basic structure of the optimization problem as problem in decision making with the necessary input of an objective function is thus evident. On the other hand a model is never an end in itself but on the basis of its simulation capacity a means to an end, for example in biotechnological optimization. From this point of view the model is a product of scientific work and thus an economic value. Equally, through its intended purpose the model exhibits a utility value. A complete evaluation of the model as a condition of rational modeling must take into account both these aspects. That is possible in principle by adding the modeling expenditure to expenditure for the realization of biotechnological processes, expressing the economic consequences of model quality as an objective function, and minimizing the specific total expenditure for the product to be produced.Biotechnological practice requires that the “optimum” model is approached by means of iterative processes. Some practical examples will make the process clear, taking into account qualitative (semantic) and quantitative (accuracy) aspects of the utility value.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 103
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 104
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 105
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 49-53 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zymomonas mobilis ATCC 53431, a fructokinase negative mutant is unable to utilize fructose as a sole carbon source for growth. At high fructose concentrations, however, fructose was converted to ethanol. The fructose uptake displayed MICHAELIS-MENTEN kinetics with an apparent Km of 185 mM fructose.Purified glucokinase from ATCC 53431 and the wild strain ATCC 29191 both exhibited fructose phosphorylating activity at high fructose concentrations with an apparent Km value of 222 mM fructose. Glucokinase substrate specificity was found not be absolute, as previously reported.
    Additional Material: 2 Ill.
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  • 106
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 41-48 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Five cellulases were fractionated from a commercial cellulase preparation (CelluclastTM) Two isoenzymes of cellobiohydrolase I (CBHI)(pI = 4.1) could be proved to be real exo-glucanases due to their activity towards MU (=methylumbelliferyl)-lactoside being inhibited by cellobiose (5 mM) and due to production of cellobiose from carboxymethylcellulose (CMC) as the sole final product.Two isoenzymes of CBHII (pI=6.15, 6.0) were shown to act as endo-glucanases because they produced glucose, cellobiose and cellotetraose from CMC and because they were not inhibited by cellobiose when decomposing MU-lactoside. Results confirm recent reports in the literature classifying CBHI and CBHII as exo-type and endo-type cellulases, respectively. Both the CBHI and the CBHII isoenzymes were shown to be active towards CMC and amorphous cellulose.CBHI and CBHII reactions could be differentiated from one another by the velocities of decomposition of CMC: CBHI acts slowly and linearly whereas CBHII acts strongly and exponentially.The fifth of the purified enzymes must be classed as a conventional endoglucanase which exhibits activity towards CMC but fails to be active towards MU-lactoside and amorphous cellulose.
    Additional Material: 6 Ill.
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  • 107
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 62-62 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 108
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 63-71 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For recombinant DNA product fermentations in the laboratory and pilot scale as well as for antibiotic fermentations at the industrial scale decision support program systems for personal computers were developed. It assists the operator to solve control and decision tasks. Applying this program the data and knowledge bases increase, which include measured and derived process data, parameters and comments of previous fermentation runs as well as algorithmic and declarative rules.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 109
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 110
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 55-61 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: High-molecular weight pectic acid with a STAUDINGER index of 210 ml/g and a degree of esterification of 3%was used as matrix material for the immobilization of Saccharomyces cerevisiae cells. In discontinuous and continuous fermentation tests the gel beads obtained exhibited the same biomass loading capacity (152-155 g dry wt. cells/kg gel) and about the same maximum specific productivity (103.0 g ethanol/kg gel · h) as alginate immobilizates. But there were distinct differences in the swelling behaviour of the two gels. Under the same experimental conditions the increase of bead volume amounted to 27% only for pectate gel in comparison to 129% for alginate gel. In continuous fermentation experiments performed in a horizontal-column packed-bed reactor with liquid recycling a mean steady-state ethanol concetration of 69.1 g/l and a mean productivity of 24.7 g ethanol/lh could be kept constant over a period of more than 10 days.
    Additional Material: 2 Ill.
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  • 111
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 73-78 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of temperature on chemostat cultures of a thermotolerant, methylotrophic Bacillus ap. are reported. In spite of the relative fastidiousness of this bacterium, overall response to a range of growth temperatures is predictable. However, anomalous results with respect to biomass yield coefficients are evident, suggesting complex and extensive endogenous and exogenous activities under some growth conditions.
    Additional Material: 4 Ill.
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  • 112
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 93-98 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Isolation of auxotrophic mutants and conjugal transfer of plasmids are successfully demonstrated in four obligate methylotrophic strains isolated at Kuwait Institute for Scientific Research (KISR)Out of 11 auxotrophic, mutants, three required methionine and two leucine. Complete transfer of plasmid R68.45 from Pseudomonasto KISR methylotrophs and vice versa have been achieved with a low transfer rate.
    Additional Material: 2 Ill.
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  • 113
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 79-84 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aim of the project BD1 (Biotechnology Data Bank 1) is to improve the data processing for research in biotechnology. In BD1, data of several classes of objects and methods are compiled. A data file conception consisting of object data files, method data files and feature data files was created, which can be easily extended to further classes, and which allows the combination of different classes of features to the same class of objects. There are two main approaches to use BD1: 1. making available informations on certain biotechnological objects, 2. the identification of unknown objects by means of their feature pattern.The microbiological data base and the possibilities of data base search are presented by example of lactic acid bacteria.
    Additional Material: 7 Tab.
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  • 114
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 85-92 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of 33 polyethoxylated nonionic tensides with various hydrophobic moieties was studied on the proteolytic activity and phase transition behaviour of papain. Tensides with longer ethyleneoxide chain markedly increased the phase transition temperatures of papain indicating possible hydrogen bond formation between the hydrophilic ethyleneoxide chain and the polar substructures of papain. The character of lipophilic moiety of tensides influences also each physicochemical parameter of papain suggesting the existence of hydrophobic interactions too. The significant positive linear correlations between the activity increasing and structure stabilizing effects of tensides make probably that the structure modification of papain accounts for its enchanced proteolytic activity.
    Additional Material: 2 Ill.
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  • 115
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 99-104 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three distinct heterotrophic eubacterial strains were isolated from mixotrophic cultures of the filamentous cyanobacterium, Spirulina maxima (Gom) Geitl. Spirulina spp. are considered to be prime candidates for the phototrophic production of biomass protein, particularly in developing countries. These cyanobacteria are extreme alkaliphiles and halophiles, making their production in arid regions promising.Most previous studies on the eubacteria which live in Spirulina culture systems have focused on determining the possible presence of pathogenic species in biomass protein. Little has been done to understand the symbiotic relationships between the cyanobacterium and its eubacterial cosymbionts. From the perspective of a heterotrophic eubacterium, autotrophic cultural systems of Spirulina have limited carbon and energy resources, being limited to cyanobacterial exudates.In this study, three eubacterial strains were isolated and studied. One strain, a Gram-negative, non-sporing, motile rod, grew exceptionally well in a mineral salts medium where only a small amount of a single low molecular weight organic compound (e.g., acetate) was supplied as sole energy source. This strain was also extremely euryresponsive with respect to salinity and alkalinity as well. Two less well-adapted eubacterial strains are also described.
    Additional Material: 2 Tab.
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  • 116
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990) 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 117
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: NO ABSTRACT.
    Type of Medium: Electronic Resource
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  • 118
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 119
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 124-124 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 120
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 107-115 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The enterobacterial outer membrane forms a bilayer. Its outer monolyer consists of lipopolysaccharides and proteins, its inner monolayer of phospholipids and proteins. It thus represents an efficient penetration barrier against hydrophobic and anionic compounds (such as detergents or hydrophobic antibiotics) and against higher molecular substances (such as proteolytic, lipolytic, and murolylic enzymes).Some of the proteins (“porins”) form channels through the outer membrane through which neutral and cationic hydrophylic compounds up to a molecular weight of about 800 can pass. Besides the porins additional transport systems have been described. They play an important part in providing the bacteria with substances necessary for their growth, i.e., phosphate, iron ions, and others.Organic polycations are able to generate more or less severe disorganizations in the outer membrane through which they can pass the bilayer (“self-promoted pathway”). Some of these polycations represent efficient antibiotics (polymyxin B, nourseothricin). Bacteria are able to protect themselves against the harmful action of these substances by changing the composition of the outer membrane.
    Additional Material: 9 Ill.
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  • 121
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 117-123 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For intact cells of A. calcoaceticus 69V susceptibility to hydrophobic agents (antibiotics, dyes) was established. The composition of its outer membrane and comparison with that of a reference strain, A. calcoaceticus CCM 5593 with a blocked hydrophobic pathway, gave no indication of phospholipid bilayer domains as the structural basis of these permeability characteristics. The outer membrane composition together with the data of time-resolved fluorescence anisotropy measurements is indicative of a high state of order of the hydrocarbon region.A. calcoaceticus 69V releases lipopolysaccharide (LPS)-rich membrane vesicles into the growth medium when grown on a hydrophobic carbon source. While the cells contain both R-form and S-form LPS, the LPS released with the vesicles is exclusively of the R-type. The same selectivity with respect to LPS composition was observed when LPS was removed from intact cells by EDTA-NaCl treatment which leads to a break-down of the barrier to hydrophobic agents in A. calcoaceticus CCM 5593.We propose that due to its physical properties, R-form LPS forms tightly packed structures within the membrane which, under certain conditions, become destabilized and liberated into the surrounding medium. As a consequence, a disturbance of the highly ordered lateral molecular arrangement might lead to altered permeability properties of the outer membrane as suggested in one of the two alternative models existing to explain permeability changes observed in deep rough mutant strains of Enterobacteriaceae.
    Additional Material: 3 Ill.
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  • 122
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 142-142 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 123
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The genus Citrobacter consists of enteric rods that utilize citrate as a sole carbon source. Citrobacter serotypes are normal intestinal inhabitants, but some of them have been reported in gastroenteritis and in extraintestinal infections.Our immumochemical studies on Citrobacter lipopolysaccharides of chemotype G comprised serotypes: 04, 027 and 036. As we found the serotypes mentioned above are distinguished in having O-specific polysaccharides that are homopolymers of β [1-2] linked 4-deoxy-D-arabinohexopyranose. In accordance with the structural indentity, the Citrobacter lipopolysaccharides showed strong serological cross-reactivity. Nevertheless, serotypes 04, 027, and 036 form separate entities, as observed in early studies of serologists. To find the reason for the immunological diversity the structural studies on lipopolysaccharide core regions of these Citrobacter serotypes were carried out. Three novel enterobacterial cores were identified. The complete cores 04 and 036 are decasaccharides differing in one structural element only: side-chain glucose is present in 04 oligosaccharide in the place of galactose in the 036. The core 027 is octasaccharide.In conclusion, our structural studies have shown hitherto that in Citrobacter chemotype G group only one O-specific polysaccharide, but three core regions, exist.
    Additional Material: 9 Ill.
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  • 124
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 125-132 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Freeze-fracturing is especially suitable for the investigation of membrane structures. In contrast to ultrathin sectioning, large areas of the membranes are exposed. The true surface of membranes, however, can be seen only after etching (vacuum sublimation of ice) because during fracturing the frozen membrane is split between the two lipid layers. The representation of the hydrophobic region of the membrane reveals particles representing integral membrane proteins or, exceptionally, micelles of membrane lipids. Special structures on microbial membranes are, e.g., regular particle arrangements, invaginations and lipid domains with a periodic pattern of curvatures. There are still many unsolved questions concerning these structures, but the occurrence or the alteration of such structures as well as the density of “etching holes” on the membrane fracture face can be used as indicators for membrane perturbations.
    Additional Material: 7 Ill.
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