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  • 1970-1974  (338)
  • 1973  (338)
  • Engineering General  (189)
  • Electron microscopy  (149)
  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural studies on the ontogenesis of the caudal neurosecretory system in the roach, Leuciscus rutilus (1973)
    Springer
    Anatomy and embryology 139 (1973), S. 207-216 
    Details
    ISSN: 1432-0568
    Keywords: Caudal neurosecretory system ; Ontogenesis ; Electron microscopy ; Teleost fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ontogenesis of the urophysial system (caudal neurosecretory system) in the roach (Leuciscus rutilus) was ultrastructurally analyzed. In newly hatched spawns the urophysial components are differentiated in the form of neurosecretory perikarya, axons and terminals and display the characteristics of a functional system although the neurohemal area is not developed. It is assumed that the system is active simultaneously with the preopticohypophysial system during ontogenesis. On the contrary, organogenesis of the urophysis is late, and only at the 14 mm stage do the neurosecretory axons first penetrate the meninx to participate in the formation of the organ. Assumed aminergic cell types associated with the urophysial system are differentiated at the time of hatching, thus indicating a functional relationship. A secretory ependyma releasing its granules into the central canal is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523639
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  • 2
    Electronic Resource
    Electronic Resource
    The fusion of the endocardial cushions in the heart of the chick embryo (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 55-75 
    Details
    ISSN: 1432-0568
    Keywords: Heart ; Endothelial cushion ; Fusion ; Cell interaction ; Electron microscopy ; Chick embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In an investigation concerning the so-called “fusion” of the atrioventricular endocardial cushions in the heart of the chick embryo the following facts were established: 1. The endocardial cushions in the chick embryo do really fuse. However, the region of fusion is less extensive than is generally accepted. 2. No regressive cellular changes were observed in the fusing endothelia with light or electron microscopy. On the contrary, mitotic activity and possibly even erythroblastic transformation were observed in the endothelium as well as in the subendothelial mesenchyme. 3. Under the light microscope, the process of fusion is characterized by interdigitation of the endothelial cells. In later stages the endothelial character of these cells disappears in such a way as to give rise to one single mesenchymal cushion mass. 4. Under the electron microscope the fusing endothelial cells form extremely long tonguelike cytoplasmic processes which protrude freely into the lumen where they may fuse with similar processes from other endothelial cells of the same side. In this way small compartments become segregated from the lumen. the plasma membrane of the processes may also fuse with the plasma membrane of the cell from which they originate. Alternating persistence and disappearance of the plasma membrane gives rise to many membrane-lined intracellular vesicles. 5. The numerous myelin figures found in and between the endothelial surfaces appear to represent the blind and empty membranous linings stripped off from cytoplasmic processes. The intracellular myelin figures are probably incorporated into the cells in the same way as described above. Apart from this form of endocytosis, signs of conventional micropinocytotic activity are present. 6. The density of endoplasmic reticulum suggests intensified protein synthetic activity, in which membrane-bound as well as free ribosomes and polysomes seem to participate. Moreover, increased mitochondrial density in the cells in the fusion region is evident. 7. Specialized junctional complexes between the plasma membranes in the form of desmosome-like junctions are relatively scarce. 8. Fusion of cytoplasmic tongues of endothelial cells with adjacent endothelial cells of the same side or of the opposite side is responsible for the exchange of large quantities of cytoplasma between endothelial cells and, as a consequence, for displacement of cell boundaries without loss of cell individuality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523365
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  • 3
    Electronic Resource
    Electronic Resource
    Über glykoproteidhaltige amöboide Zellen im embryonalen Hühnergehirn (1973)
    Springer
    Anatomy and embryology 142 (1973), S. 341-358 
    Details
    ISSN: 1432-0568
    Keywords: Glycoproteid containing cells ; Microglia ; Brain development ; Chicken ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Zur Zeit der embryonalen und fetalen Entwicklung des Hühnchens treten vorübergehend runde, vacuolisierte, freie Zellen auf, die sich durch starken Gehalt und Glykoproteiden auszeichnen. Nachweis mit der Perjodsäure-Bisulfit-Aldehydthionin-Methode nach Specht (1970). Der Vergleich von Entwicklungsstadien zwischen dem 4. und dem 17. Bebrütungstag zeigt, daß die Zellen im Mesenchym entstehen und mit großer Wahrscheinlichkeit sowohl über das Ventrikelsystem als auch über die äußere Hirnoberfläche in das Gehirn einwandern. Die Zellen werden hauptsächlich in aufgelockerten, flüssigkeitsreichen Hirngebieten gefunden. Vermutlich geht die vorübergehende Auflockerung dieser Hirngebiete, die als Reservevolumen für nachfolgende histogenetische Wachstumsprozesse (Axonwachstum, Myelinisierung) verstanden wird, auf die Träger der PBA-positiven Zellen zurück (Ausschleusung von Glykoproteiden aus den PBA-positiven Zellen). Im gleichen Zeitraum bilden auch Ependymzellen Glykoproteide.
    Notes: Summary Round, vacuolated amoeboid cells with a high content of glycoproteids appear temporarily during the embryonic development of the chicken's brain. The cells were identified by the periodic-acid-bisulfit-aldehydthionine-method (Specht, 1970). A comparison of the developmental stages (4.–17. day of incubation) of the cells show that they originate in the mesenchym and probably immigrate by way of the ventricles as well as from the outer brain surface. The cells are mainly found in brain areas containing large, loosely structured extracellular spaces. The large extracellular gaps of these areas are probably developed in response to glycoproteid secretion by PBA-positive cells, as a preparatory measure for subsequent histogenetic growth processes (axon growth with myelinisation). At the same time glycoproteids are also produced by ependymal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519136
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  • 4
    Electronic Resource
    Electronic Resource
    The ultrastructure of lamellated mechanoreceptors in the skin of reptiles (1973)
    Springer
    Anatomy and embryology 143 (1973), S. 81-94 
    Details
    ISSN: 1432-0568
    Keywords: Electron microscopy ; Mechanoreceptors ; Skin ; Reptiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lamellierte Receptoren in der Haut von Reptilien werden licht- und elektronenmikroskopisch beschrieben. Rekonstruktionen aus Schnittserien haben gezeigt, daß sie 1. als freie lamellierte Endigungen, 2. als lamellierte eingekapselte Endigungen und 3. als lamellierte eingekapselte Endigungen mit Kapselraum vorkommen. Sie haben eine Spindelform und können bis zu 0,5 mm lang sein. Die innere Lamellierung des eingekapselten Receptors mit Kapselraum vom Kaiman zeigt strukturelle Ähnlichkeiten zur inneren Hülle des Vater-Pacinischen Körperchens. Das Lamellensystem der Receptoren von Natter und Waran zeigt Parallelen zum Bau des inner core des Herbstschen Körperchens der Vögel. Die lamellierten Receptoren bilden einen regelmäßigen Bestandteil der sensiblen Innervation von Tastpunkten der Reptilienhaut.
    Notes: Summary Lamellated receptors in the skin of reptiles were studied with the light and electron microscope. As revealed by reconstructions of serial sections, there are three types of lamellated receptors: 1. lamellated free endings, 2. lamellated encapsulated endings and 3. lamellated encapsulated endings with a “fluid-filled” capsule space (outer core). The spindle shaped endings have a length of up to 0.5 mm. In Caiman, the lamellae (inner core) of the encapsulated receptor with a capsule space have a structure similar to that of the inner core of the Vater-Pacinian corpuscle. The lamellation (inner core) of the terminals of Natrix and Varan shows similarities with the inner core of the Herbst corpuscle. Lamellated receptors usually form an element of the sensory innervation of touch papillae in the reptile skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519912
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  • 5
    Electronic Resource
    Electronic Resource
    Helical, striated rootlets in the midpiece of a teleost fish spermatozoon (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 11-17 
    Details
    ISSN: 1432-0568
    Keywords: Electron microscopy ; Cross-striated fibers ; Rootlets ; Midpiece ; Fish spermatozoa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the 45 μ long midpiece of the spermatozoon of Pantodon buchholzi nine helical, cross-striated fibers run from one end to the other and parallel nine helical mitochondrial threads; both fibers and mitochondrial threads surrounding the axoneme. The fibers have a major period about 750–900 Å and are very similar to ciliary rootlets and other cross-striated structures in terms of which they are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520714
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructure of myotendinous junctions in Myxine and rat specializations between the plasma membrane and the lamina densa (1973)
    Springer
    Anatomy and embryology 142 (1973), S. 91-101 
    Details
    ISSN: 1432-0568
    Keywords: Myotendinous junction ; Myxine and rat ; Lamina densa ; Plasma membrane ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Myotendinous junctions in the parietal muscle of the Atlantic hagfish (Myxine glutinosa, L.) and in the diaphragm of rat were studied with the electron microscope. The ends of muscle fibers posses deep tube-like invaginations and also finger-like processes. Terminal I-filaments of the myofibrils attach to the plasma membrane of the invaginations. Collagen fibrils of the tendon attach to the external aspect of the lamina densa. The lamina densa and also collagen fibrils continue into the invaginations. Specializations are present in the zone between the plasma membrane and the lamina densa of the invaginations, being most conspicuous in Myxine. These consist of 60 Å wide spine-like or thread-like profiles 150 Å apart, connecting with both the outer leaflet of the plasma membrane and the lamina densa. Spatially, they form parallel circular ridges oriented transversely in stacks along the invaginations. Similar spine-like profiles are also discernible in the invaginations of rat muscle fibers. In rat, however, their spatial arrangement (ridges or fibrils) remains unknown. These specializations may serve to attach the lamina densa to the plasma membrane, and may be important for the transmission of force from muscle fibers to tendon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519878
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural observations on cell necrosis during formation of the neural tube in mouse embryos (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 251-264 
    Details
    ISSN: 1432-0568
    Keywords: Closure of neural tube ; Cell necrosis ; Normal mouse embryos ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Spontaneous cell death in the developing brain of 8.5–9 day old mouse embryos has been investigated with the electron microscope. Before closure of the neural tube, areas of cell death are found at the neuro-somatic junction. After closure of the neural tube degenerating cells are found in the dorsal midline of the prospective diencephalon. Ultrastructurally, cell degeneration is marked by chromatin condensation, increase in electron density of the structures in the cytoplasm and, in later stages, by a marked pycnosis of the dying cell. After fragmentation, the necrotic material as well as entire pycnotic cells are phagocytized and digested by cells of the neuroepithelium. An invasion of macrophages has not been observed. The significance of cell necrosis is discussed in relation to the normal formation of the neural tube and to the occurrence of certain induced malformations (exencephalies).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519046
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  • 8
    Electronic Resource
    Electronic Resource
    Morphogenesis of the myoneural junctions after immobilization of the muscle in the rat (1973)
    Springer
    Anatomy and embryology 143 (1973), S. 1-12 
    Details
    ISSN: 1432-0568
    Keywords: Neuromuscular junction ; Development ; Cholinesterases ; Muscle ; Immobilization ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immobilization of the tibialis anterior muscle of the newborn rat was caused by amputation of the hind limb at the talocrural joint. Subsequent muscular atrophy was measured by weighing the muscle and the myoneural morphogenesis in the disused muscle was studied in light and electron microscope. The acetylcholinesterase (AChE; E.C.3.1.1.7) and nonspecific cholinesterase (Ns. ChE; E.C.3.1.1.8) activity at the myoneural junction was followed histochemically. It was observed that the myoneural morphogenesis proceeded normally in the immobilized and atrophied muscle. Cholinesterase activities at the developing myoneural junction were identical on both the immobilized and the contralateral control side. The myoneural morphogenesis may be solely controlled by the nerve since, at the light of the present observations, the abnormally diminished function of the muscle seems to have no effect on the myoneural development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519906
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  • 9
    Electronic Resource
    Electronic Resource
    Schwerer Arzneimittel-Ikterus durch das Tuberculostaticum Isonicotinsäurehydrazid (INH) bei einem Kleinkind mit Phenylketonurie (1973)
    Springer
    European journal of pediatrics 115 (1973), S. 49-58 
    Details
    ISSN: 1432-1076
    Keywords: Phenylketonuria ; Seizures ; Isoniazid ; Jaundice ; Electron microscopy ; Degeneration of organelles in liver cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei einem jordanischen Kleinkind, das wegen einer Phenylketonurie mit cerebralen Krampfanfällen seit 1 Jahr mit Primidon und Ospolot behandelt und seit 7 Monaten mit einer phenylalaninarmen Diät unter Zusatz eines Aminosäurengemisches ernährt wurde, entwickelte sich nach 3 monatiger INH-Medikation ein hepatocellulärer Arzneimittelikterus. Dieser erwies sich klinisch und histologisch als reine Form einer toxischen Hepatose ohne entzündliche Symptome. Die elektronemmikroskopische Untersuchung von Leberbiopsiematerial 2 Wochen nach dem Höhepunkt des Ikterus (28 mg% Gesamtbilirubin im Serum) erlaubte den Nachweis eines intracellulären Transportdefektes des bereits konjugierten Bilirubins in den Leberepithelien. Das Pigment wurde analog dem Dubin-Johnson-Syndrom in peribiliären Lysosomen gespeichert. Weitere für die Pathogenese aufschlußreiche Befunde wurden an den Organellen der Hepatocyten erhoben: Hydropische Schwellung des hypertrophierten glatten endoplasmatischen Reticulums bei gleichzeitiger Reduktion des rauhen endoplasmatischen Reticulums, Glykogenschwund und Degeneration der Mitochondrien. Bei der Kontrollbiopsie nach 1/2 Jahr waren alle Befunde bis auf die lysosomale Speicherung normalisiert.
    Notes: Abstract After being treated with isonicotinic acid hydrazine (INH) for 3 months a 3-year-old Jordanian boy with phenylketonuria developed severe hepatocellular jaundice. In addition to INH the patient had received Primidone and Ospolot® for 1 year and a PKU diet for 7 months without any side effects. The liver biopsy 2 weeks after the peak of jaundice revealed no signs of hepatitis on light microscopy. An electron microscope investigation showed a defect in the intracellular transport system of bilirubin; bile pigment was stored in peribiliar lysosomes in the same way as in the Dubin-Johnson Syndrome. The smooth endoplasmatic reticulum was increased and vacuolized, whereas rough endoplasmatic reticulum, mitochondria, and glycogen were reduced. In a control biopsy nearly 6 months after the jaundice the morphologic alterations of the liver had normalized with the exception of the lysosomal changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00438990
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  • 10
    Electronic Resource
    Electronic Resource
    Licht- und elektronenmikroskopische Untersuchungen an der Zona glomerulosa des Hundes nach Behandlung mit Spirolacton (1973)
    Springer
    Research in experimental medicine 159 (1973), S. 321-332 
    Details
    ISSN: 1433-8580
    Keywords: Spirolactone ; Aldosterone ; Zona glomerulosa ; Electron microscopy ; Spirolacton ; Aldosteron ; Zona glomerulosa ; Ultrastruktur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Wirkung von Spirolacton auf die Zona glomerulosa des Hundes wurde licht- und elektronenmikroskopisch untersucht. Die an Kontroll- und Versuchstieren erhobenen Befunde werden miteinander verglichen. 1. Lichtmikroskopisch werden bei Kontroll- und Versuchstieren zwei unterschiedlich stark anfärbbare Zelltypen beobachtet. Bereits nach 10tägiger Behandlung mit Spirolacton läßt sich eine beträchtliche Dickenzunahme der Zona glomerulosa nachweisen. Mit den angewandten Färbemethoden lassen sich in den beiden Zelltypen keine den „Spironolacton-Körpern“ ähnliche Einschlüsse darstellen. 2. Elektronenmikroskopisch zeigen die beiden Zelltypen deutliche Unterschiede in der Feinstruktur; wegen ihrer unterschiedlichen elektronenoptischen Dichte werden sie als helle und dunkle Zellen bezeichnet. Im Cytoplasma der dunklen Zellen kommen 1–4 lamelläre Körper vor, die jedoch nicht den beim Menschen beobachteten „Spironolacton-Körpern“ ähnlich sind. Nach Behandlung mit Spirolacton weisen die hellen und die dunklen Zellen (einschließlich ihrer lamellären Körper) gegenüber Kontrolltieren keine feinstrukturellen Unterschiede auf. Es wird diskutiert, ob die hellen und dunklen Zellen in der Zona glomerulosa unterschiedliche Aktivitätszustände ein und derselben Zellart darstellen. Ebenso wird die mögliche Funktion der lamellären Körper im Vergleich zu den Ergebnissen anderer Autoren erörtert.
    Notes: Summary The effect of spirolactone on the zona glomerulosa was studied in dogs by light and electron microscopy. Results of untreated animals are compared with those pretreated with spirolactone for several days. Light microscopy in control as well as in experimental animals disclosed two different types of cells as far as their staining properties were concerned. With the staining techniques utilized in this study cellular inclusions, so-called spironolactone bodies as previously reported by others, were not seen. The width of the zona glomerulosa, however, was found to be significantly increased in treated animals. Electron microscopy revealed the two respective cell types to be definitely different. Due to their electron density they are referred to her as light and dark cells. Within the cytoplasm of the dark cells laminated inclusions were seen, ranging in size from 1 to 4 µm. These inclusions are thought to be not identical with socalled spironolactone bodies. Spirolactone did not affect the ultrastructural appearance of either light or dark cells, when compared with control, this is also true for the laminated inclusions. It is suggested that the light and dark cells represent the same type of cell at different activity levels. Finally, the functional significance of the laminated inclusions is discussed in view of findings reported elsewhere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851606
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  • 11
    Electronic Resource
    Electronic Resource
    A morphologic and histochemical study of biliary atresia in lamprey liver (1973)
    Springer
    Cell & tissue research 136 (1973), S. 85-96 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Lampetra ; Physiological biliary atresia ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A morphologic and histochemical study was carried out on the liver of larval and adult lampreys at the optical and electron microscopic level. In the larva the liver is composed of blind ending single cell thick tubules of hepatocytes. The tubular lumina provided with microvilli are morphologically comparable with the canalicular lumens of the higher species of animals. The cytoplasm of the hepatocytes contains numerous inclusions with heterogeneous appearance and crystalline material. The biliary system is composed of numerous bile ductules and ducts. In the adult lamprey, the biliary system has disappeared. The hepatocytes loose their tubular arrangement and the characteristic differentiation of their biliary pole. In contrast to previous reports in the literature the presence of bile pigment in the adult lamprey liver could not be demonstrated with any histochemical technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307681
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  • 12
    Electronic Resource
    Electronic Resource
    Zur Feinstruktur einer elektrischen Synapse (1973)
    Springer
    Cell & tissue research 136 (1973), S. 139-152 
    Details
    ISSN: 1432-0878
    Keywords: Giant fiber ; Earthworm ; Septum ; Electrical synapse ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die erneute elektronenmikroskopische Untersuchung der dorsalen Riesenfasern der Lumbriciden Lumbricus terrestris (L.t.) und Eisenia foetida (E.f.) zeigt, daß es sich bei den segmentalen Septen um Strukturen bisher unbekannter Komplexität handelt. Bei beiden Tierarten beträgt der Septalspalt nur 65 Å (E.f.) bzw. 75 Å (L.t.) und ist deshalb als „gap junction“ anzusprechen. Daneben fallen folgende Differenzierungen am Septum auf: „septate junctions” (L.t. und E.f.), „intermediate junctions“ (L.t. und E.f.), beidseitig am Septum gelegene und nicht von einer Membran umschlossene Membranappositionen (E.f.), sowie einseitig am Septum gelegene „dense projections“ (L.t.). Bei L.t. kommen außerdem auf beiden Seiten des Septums Vesikel vor, die von einer Elementarmembran umschlossen sind und nach Größe (φ ca. 575 Å), Lage, Haufenbildung und elektronenoptischem Habitus den Vesikeln chemisch übertragender Synapsen gleichen. Die Befunde werden hinsichtlich ihrer möglichen Bedeutung bei der elektrischen Übertragung und als Indizien für das Vorliegen einer gemischten Synapse diskutiert.
    Notes: Summary Electronmicroscopical examination of the dorsal giant fibers in the earthworms Lumbricus terrestris (L.t.) and Eisenia foetida (E.f.) reveals that their segmental septa are structures of so far unknown complexity. In both species the extracellular cleft between the two axon membranes of the septum amounts to only 65 Å (E.f.) and 75 Å (L.t.) respectively and is therefore regarded as “gap junction”. The following other structural differentiations of the septum were observed: “septate junctions” (E.f. and L.t.), “intermediate junetions” (E.f. and L.t.), densities apposed to both sides of the septum and not surrounded by a membrane (E.f.), and densities resembling “dense projections” on one side of the septum only (L.t.). In addition the septa of L.t. show vesicles on both sides which are bounded by a unit membrane and resemble the vesicles of chemically transmitting synapses in size (φ ca. 575 Å), location, accumulation, and electronoptical habitus. The significance of the findings in regard to electrotonic transmission and the possible existence of a mixed synapse is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307685
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  • 13
    Electronic Resource
    Electronic Resource
    Direct demonstration of eight-fold symmetry in nuclear pores (1973)
    Springer
    Cell & tissue research 136 (1973), S. 183-190 
    Details
    ISSN: 1432-0878
    Keywords: Nuclear pore ; Amphibian oocyte ; 8-fold symmetry ; Fibrous network ; Electron microscopy ; Negative staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pictures, in front view, are presented of the nuclear pores from the oocytes of the newt Taricha granulosa. Negative staining is used. It is directly visible, on a substantial proportion of the pores, that the number of subunits in the annulus is 8. This conclusion had been reached earlier by other writers, who had used the rotation technique to ascertain the radial symmetry. The rotation technique is known to be very unreliable, though on this occasion had produced the correct result. A fibrous mesh network, connecting the subunits of separate pores is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307439
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  • 14
    Electronic Resource
    Electronic Resource
    The ultrastructure of the pars distalis of the hypophysis in males of Triturus cristatus carnifex Laur., treated with the antiandrogen cyproterone acetate (1973)
    Springer
    Cell & tissue research 137 (1973), S. 209-221 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Male ; Triturus cristatus carnifex Laur. ; “Globular basophil cells” ; Antiandrogen treatment ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adenohypophysis of the newt Triturus cristatus carnifex Laur. shows three types of cells: 1) cells with granules of about 350–550 mμ in diameter, 2) cells with small granules of 200–250 mμ in diameter and globules with a cristal-like arrangement containing cylinders with a diameter of about 960 Å and 3) cells containing small granules only. The AA. discuss the ultrastructural changes of the gland and the modifications of sexual secondary characters (S.S.C.) in animals given Cyproterone acetate (1/2 mg every three days). The animals have been treated for a period of time varying between 3 and 5 months, starting in October-November, when S.S.C. begin to develop again. At the end of the treatment the newths showed a loss of S.S.C., and the ultrastructure of the adenohypophysis resembled that of castrated animals, i.e.: great swelling of R.E.R. and partial degranulation of glycoprotein secreting cells which contain the 200 mμ granules and the globules. The S.E.R. showed also swelling and hyperactivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307431
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  • 15
    Electronic Resource
    Electronic Resource
    Evolution des glandes prothoraciques de larves permanentes de Locusta migratoria obtenues par irradiation sélective du tissu hématopoïétique (1973)
    Springer
    Cell & tissue research 137 (1973), S. 387-397 
    Details
    ISSN: 1432-0878
    Keywords: Insect molting ; Hemocytopoietic tissue ; Prothoracic glands ; Irradiation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It has been shown in previous studies that the endocrine control of molting is blocked by selective X-irradiation of the hemocytopoietic tissue of young last instar larvae of Locusta migratoria, the treated animals surviving as larvae for months without ever initiating a molt. The aim of the present study is to determine, on both a morphological and a physiological basis, if the block is related to a precocious functional involution of the prothoracic glands, known to elaborate a molting hormone. The prothoracic glands of normal animals clearly show morphological signs of functional involution during the late fifth (last) larval instar and degenerate completely some days after the imaginal molt. In the prothoracic glands of X-ray treated animals (selective irradiation of the hemocytopoietic tissue, the prothoracic glands being shielded), the signs of intense secretory activity persist up to 40 days following the irradiation. No signs of involution could ever be noticed in the prothoracic glands of such larvae. The irradiation of the hemocytopoietic tissue thus not only blocks molting, but also prevents the normal involution of the prothoracic glands. Extirpation of the prothoracic glands in young larvae of Locusta is known to result in the inhibition of the subsequent molt. Implantation of prothoracic glands from normal donors of the same age restores molting in fifth instar larvae previously deprived of their own glands. Using these results in physiological assays for the implanted prothoracic glands, we chose as donors irradiated animals of different ages and young normally developing adults. The prothoracic glands of irradiated animals fully retain their physiological activity after the X-ray treatment and are able to induce molting in larvae deprived of their own glands. It appears that the radiosensitive cells of the hemocytopoietic tissue of Locusta produce a substance which, while having no overt influence on the functioning of the prothoracic glands, is necessary for molting and probably acts in synergy with the prothoracic factor (molting hormone).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307211
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  • 16
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    Electronic Resource
    Das unterschiedliche Bild organspezifischer Strukturen im Glomus caroticum und Nebennierenmark (1973)
    Springer
    Cell & tissue research 138 (1973), S. 133-154 
    Details
    ISSN: 1432-0878
    Keywords: Carotid body ; Adrenal medulla ; Rabbit ; Osmiophilic vesicles ; Cat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die organspezifischen Strukturen im Glomus caroticum und Nebennierenmark zeigen unter verschiedenen experimentellen Bedingungen ein unterschiedliches Bild. So läßt sich unter anderem durch Modifizierung der Fixationsmedien (Glutaraldehyd, Osmiumsäure in Kombination mit verschiedenen Puffern) eine optimale Differenzierung der zellulären Strukturelemente von Glomuszellen erreichen. An den osmiophilen Vesikeln fällt eine starke Variabilität ihres Erscheinungsbildes auf. Feinstrukturelle Unterschiede werden in Elektronendichte des Vesikelinhaltes, Größe und Ausbildung des Hofes sowie in Aufbau und Verlauf der Membran deutlich. Auf Grund dieser Kriterien lassen sich im wesentlichen 4 Haupttypen der Granula von seltener vorkommenden Sonderformen abgrenzen.
    Notes: Summary The organospecific structures of the carotid body and the adrenal medulla show a different appearance under various experimental conditions. Thus, it is possible to obtain an optimal differentiation of the cellular structure elements of the carotid body cells by modifying the fixatives (glutaraldehyde, osmic acid, in combination with various buffer systems). Especially the osmiophilic vesicles show a great variability in their appearance. Ultrastructural differences become evident in the electron-density of the vesicle content, in the size of the electron-lucent space (halo of the dense core), as well as in the structure and conduct of the membrane. Based on these criteria four main types of granules can be said to occur in the carotid body. These can be distinguished from more uncommon special types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307083
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  • 17
    Electronic Resource
    Electronic Resource
    Ultrastructural study on contractile structures in mammalian nephron (1973)
    Springer
    Cell & tissue research 140 (1973), S. 101-124 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Contractile structures ; Embryonic metanephros ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytoplasmic filaments have been observed in the cells of normal and pathological kidneys. These filaments are usually grouped into bundles anchored to electron dense bodies underlying the cell membrane. In the embryonic human metanephros the filaments are found within the cells of different portions of the nephron at various stages of development. They appear first in the podocytes, almost simultaneously in the Bowman's capsule and tubular cells, then in the “mesangial cells”, and finally in the cells of the media of the afferent glomerular and interlobular arterioles. The presence of filaments and their attachment bodies in the mammalian nephron suggests that the podocytes and the so-called mesangial cells have a contractile activity, thus representing an intraglomerular apparatus which regulates the intravascular pressure, blood flow and filtration rate in the glomerular capillaries, whilst the contractile activity of the Bowman's capsule and proximal, distal, and collecting tubules, could facilitate the progression of the filtrate. The increase in number of the filaments in some pathological conditions is probably related to the functional changes of the intraluminal pressure in the glomerular capillaries, in the Bowman's space, and in the tubular lumen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307061
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  • 18
    Electronic Resource
    Electronic Resource
    The fine structure of the guinea-pig muscle spindle (1973)
    Springer
    Cell & tissue research 140 (1973), S. 357-368 
    Details
    ISSN: 1432-0878
    Keywords: Muscle spindle ; Guinea-pig ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nuclear bag and nuclear chain intrafusal fibres are present in guinea-pig muscle spindles. Unlike muscle spindles in other species two types of nuclear chain fibre seem to be present. The electron microscopical appearance of one type of nuclear chain fibre is similar to that of nuclear bag fibres. It is suggested that under tension the nuclei of small nuclear bag fibres become sufficiently displaced to form nuclear chain-like fibres. The frequent occurrence of fibres which combine some of the properties of both nuclear bag and nuclear chain fibres indicates the possible occurrence of a third type of intrafusal fibre. The sensory innervation of guinea-pig muscle spindles is similar to that of the cat and the rat. Three types of motor nerve ending which could be classified according to the complexity of their subneural apparatus were seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307023
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  • 19
    Electronic Resource
    Electronic Resource
    Über die Glia im Hypoglossuskern der Ratte nach Axotomie (1973)
    Springer
    Cell & tissue research 141 (1973), S. 427-442 
    Details
    ISSN: 1432-0878
    Keywords: Hypoglossal nucleus (Rat) ; Axotomy ; Glia ; Microglia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Es wird unter besonderer Berücksichtigung der Glia über den Feinbau des Hypoglossuskerns normaler Albinoratten und nach Axotomie berichtet. Untersucht wurden Tiere vom 12.–90. Lebenstag. Folgende Ergebnisse sind neu: Weder bei jungen noch bei erwachsenen Ratten kommt im Hypoglossuskern Mikroglia vor. Etwa ab. 3. Tag nach Axotomie kann bei erwachsenen Tieren Mikroglia nachgewiesen werden. Sie entsteht aus aktivierten und proliferierten Oligodendrocyten und pericytenähnlichen Bindegewebszellen. Die Mikroglia umhüllt die Nervenzellen. Eine Aktivierung und Hypertrophie von Astrocyten erfolgt etwa ab 8. Tag nach Axotomie. Von diesem Tage an drängen sich Astrocytenfortsätze zwischen Mikroglia und Nervenzelloberfläche. Die Mikroglia beginnt zu degenerieren. Bei Jungtieren bewirkt die Axotomie nur geringgradige Veränderungen. Schon beim Normaltier findet man aktive Oligodendroglia und Astrocyten sowie häufig pericytenähnliche Bindegewebszellen.
    Notes: Summary The ultrastructure of the Nucleus hypoglossi in normal albino rats and following axotomy is described with special reference to the glia. Animals 12 to 90 days old were investigated. The following results are new: Normally in young and adult rats microglia is absent in the nucleus. Following axotomy from the 3rd day no microglia can be demonstrated in adult rats. It originates from activated and proliferated oligodendrocytes and pericyte-like cells of the vascular connective tissue. These cells surround the neurons. An activation and hypertrophy of astrocytes is visible from the 8th postoperative day on. Astrocyte processes make contacts with the perikarya and separate the microglia from the neurons. Microglia cells then show signs of degeneration. In young animals alterations following axotomy are only sparse. Already normally activated oligodendrocytes and astrocytes as well as pericyte-like connective tissue cells can be seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307415
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  • 20
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    Electronic Resource
    Vinblastine-induced paracrystals and unusually large microtubules (macrotubules) in rat renal cells (1973)
    Springer
    Cell & tissue research 141 (1973), S. 443-458 
    Details
    ISSN: 1432-0878
    Keywords: Microtubules ; Vinblastine ; Kidney tubules ; Podocytes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vinblastine sulfate was administered to adult rats by intravenous injections. Kidney cortex was fixed after 1, 2, or 5 hours of treatment and studied by routine transmission electron microscopy. In control animals, cells of distal convoluted tubules possessed numerous microtubules with an average diameter of 280 Å. In treated animals, the microtubules of these cells were reduced in number, and paracrystalline inclusions characteristic of vinblastine treatment were common. Macrotubules (570 Å average diameter) were also present and often were seen close to, or in apparent continuity with, paracrystals. Since the work of others indicates that vinblastine-induced paracrystals contain microtubular protein (tubulin), observation of continuities between paracrystals and macrotubules is interpreted as evidence that macrotubules are also composed of tubulin and that macrotubules may become incorporated into paracrystals. Unlike the ordinary microtubules of cells of the distal tubules, vinblastine-induced macrotubules exhibited cross-striations in longitudinal view and subunit structure in cross section. Macrotubules and paracrystals were also observed in cells of the proximal convoluted tubule, mesangium, glomerular endothelium, parietal epithelium of Bowman's capsule, and visceral epithelium of Bowman's capsule. Continuities between macrotubules and paracrystals, although relatively common in occurrence in distal tubule cells, were only rarely seen in the other kinds of cells examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307116
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  • 21
    Electronic Resource
    Electronic Resource
    Evidence for basal secretion in the subcommissural organ of the adult rabbit (1973)
    Springer
    Cell & tissue research 142 (1973), S. 223-239 
    Details
    ISSN: 1432-0878
    Keywords: Subcommissural organ (SCO) ; Basal secretion, Lateral secretion ; Extracellular spaces (ECS) ; Innervation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphological evidence is presented supporting the possibility of basal secretion into hypendymal capillaries of the adult rabbit subcommissural organ (SCO). The synthetic apparatus of the SCO cell is described as well as the heterogeneous granules and vesicles which are concentrated in the basal processes bordering a widened perivascular space. The origin of the electron dense granules, of which two fairly distinct subgroups are found, is discussed. A binding of secretory sacs to the lateral plasma membrane is seen. The possibility of a lateral secretion is supported by the presence of a system of extracellular channels between SCO cells which are filled with a flocculent material resembling that of the secretory sacs. Nerve perikarya which are separated from the SCO by only a few glial fibers are demonstrated. Synapses are described in nerve fascicles bordering on the hypendymal capillaries. The possibility of an innervation of the hypendymal region is discussed as well as possible nervous connections with the pineal gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307034
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  • 22
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    Electronic Resource
    Saisonbedingte Veränderungen in der Morphologie der Leydigzellen von Rana esculenta (1973)
    Springer
    Cell & tissue research 142 (1973), S. 367-386 
    Details
    ISSN: 1432-0878
    Keywords: Testicular interstitial cells ; Rana esculenta ; Seasonal changes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Saisonabhängige Veränderungen in der Morphologie der Leydigzellen von Rana esculenta wurden über den Zeitraum eines Jahres in Licht- und Elektronenmikroskop untersucht. In den Monaten November bis Februar kommen im Zytoplasma der Leydigzellen kleine Mitochondrien des tubulären Typs, Vesikel des glatten endoplasmatischen Retikulum und freie Ribosomen vor, Fettvakuolen treten nur vereinzelt auf, der Golgikomplex ist gering entwickelt. In der Zeit von März bis Juni nimmt die Zahl der Zellorganellen zu. Es erscheinen in Gruppen angeordnete Lysosomen und außer den kleinen Mitochondrien Riesenmitochondrien, des Golgifeld ist größer, die Fettvakuolen sind vermehrt. Sowohl die kleinen als auch die Riesenmitochondrien enthalten Kristalle, die aus osmiophilen globulären Einschlußkörpern hervorzugehen scheinen. In den Monaten Juni/Juli ist fast der ganze Zelleib einer Leydigzelle von Fettvakuolen ausgefüllt. Die Zahl der Riesenmitochondrien und Lysosomen verringert sich, das Golgifeld ist wieder unauffällig. Eine Beziehung zwischen der Vermehrung und Entfaltung der Zellorganellen und der in der Literatur angegebenen Phase der gesteigerten Steroidproduktion konnte festgestellt werden. Das Auftreten der Riesenmitochondrien und Lysosomen und ihre Bedeutung für die saisonbedingte Entwicklung und Involution der Leydigzellen wird diskutiert.
    Notes: Summary Seasonal changes in the structure of the testicular interstitial cells in Rana esculenta were investigated. Throughout November–February, small mitochondria of the tubular type, visicles of the smooth endoplasmic reticulum and free ribosomes predominate and the cytoplasm contains few lipid droplets and a poorly developed Golgi apparatus. During the period from March to June the cell organelles increase in number. There are numerous aggregated lysosomes and conspicuous accumulations of lipid droplets. Besides small mitochondria increasing numbers of giant mitochondria occur and the Golgi complex is enlarged. The small and giant mitochondria contain crystals which seem to originate from osmiophilic globular inclusion bodies. During June and July the whole cytoplasm may be filled with lipid droplets. Concomitantly there is a decrease in the number of lysosomes and giant mitochondria and the Golgi apparatus is in a state of involution. Possible correlations between the development of the cell organelles and the period of increased steroid production are pointed out. The appearance of giant mitochondria and lysosomes and their importance for seasonal Leydig cell development and involution are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307358
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  • 23
    Electronic Resource
    Electronic Resource
    Ultrastructure of four types of striated muscle fibers in the atlantic hagfish (Myxine glutinosa, L.) (1973)
    Springer
    Cell & tissue research 143 (1973), S. 273-290 
    Details
    ISSN: 1432-0878
    Keywords: Muscle fiber types ; Atlantic hagfish (Myxine glutinosa, L.) ; Myosatellite cells ; Intranuclear rodlets ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four types of striated muscle fibers with distinctive ultrastructure were defined in the Atlantic hagfish (Myxine glutinosa, L.): “white”, “intermediate”, and “red” fibers of m. parietalis, and “red” fibers of m. craniovelaris. “White” fibers are thick, contain very few mitochondria and fat vacuoles, and possess distinct and separate myofibrils with thin Z-disks and distinct M-lines. “Intermediate” fibers are thinner, possess largely similar myofibrils that often are even better separated due to a higher content of fat vacuoles and especially mitochondria and glycogen granules. “Red” fibers of m. parietalis contain large amounts of mitochondria, fat vacuoles, and glycogen granules. Their myofibrils possess M-lines, and although branching more, the myofibrils of “red” fibers conform with a “Fibrillenstruktur” pattern like those of “white” and “intermediate” fibers. “Red” fibers of m. craniovelaris are very thin, possess many smaller fat vacuoles, and large amounts of mitochondria and glycogen granules. The myofibrils are significantly thinner than in m. parietalis fibers, run as quite independent well separated units, possess thicker Z-disks, and lack M-lines. Large amounts of myosatellite cells are associated with these “red” fibers. Triads are located near A/I-junctions in all four fiber types and occur irregularly, the density of triads being different in the various fiber types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307482
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  • 24
    Electronic Resource
    Electronic Resource
    The terminal neuromuscular junctions of lower chordates (1973)
    Springer
    Cell & tissue research 143 (1973), S. 495-504 
    Details
    ISSN: 1432-0878
    Keywords: Neuromuscular junction ; Teleosts and amphibia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the terminal neuromuscular junctions found close to the insertions of myotomal muscle fibres in teleost fish and amphibia is described, and it is concluded that in both groups, the pattern of innervation is essentially similar. Different degrees of complexity are described, both in the terminal nerve branches, and in the sub-junctional apparatus. It is suggested that the terminal pattern of innervation is an adaptation to the requirements of rapid swimming.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306768
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  • 25
    Electronic Resource
    Electronic Resource
    New types of endoplasmic reticulum in neurons of the paracervical ganglion of the rat (1973)
    Springer
    Cell & tissue research 143 (1973), S. 505-515 
    Details
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Paracervical ganglion (Rat) ; Neurosecretion ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two new types of structure of the cytoplasm of an autonomic neuron were described: (1) Inclusions of the rough ergastoplasmic reticulum, which were named “ergastosomes”, contain finely granular material with moderately electron opaque staining with both glutaraldehyde-OsO4 and KMnO4 fixations. A short comment was made on the nature of the contents of the ergastosomes. (2) Tubular formations of endoplasmic reticulum were found in the axons and/or dendrites of the paracervical ganglion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306769
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  • 26
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    Electronic Resource
    The capillaries of the middle ear mucosa in the guinea pig (1973)
    Springer
    Cell & tissue research 143 (1973), S. 517-526 
    Details
    ISSN: 1432-0878
    Keywords: Middle ear ; Guinea pig ; Capillary permeability ; Horseradish peroxidase ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The middle ear capillaries of the guinea pig have fenestrated endothelium, and the intercellular clefts are closed by tight junctions. Intracardially injected horseradish peroxidase penetrates the fenestrae of the endothelium and gains access to the extra-cellular space beneath the epithelium, and the intercellular clefts of the epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306770
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  • 27
    Electronic Resource
    Electronic Resource
    Untersuchungen über das Längenwachstum der Skelettmuskulatur von Fischen (1973)
    Springer
    Cell & tissue research 143 (1973), S. 587-596 
    Details
    ISSN: 1432-0878
    Keywords: Fish muscle (Macropodus opercularis) ; Myofibril ; Longitudinal growth ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ein Längenwachstum der Myofibrillen erscheint an terminalen, an das Interstitium grenzenden Myofibrillenabschnitten möglich. Diese Myofibrillenabschnitte weisen unterschiedliche Längen auf. Am kleinsten terminalen Myofibrillenabschnitt ist die Ribosomenkonzentration am höchsten. Mit zunehmender Verlängerung des Abschnittes erkennt man Aktinfilamente, die zwischen den dicht gelagerten Ribosomen liegen. Erreicht der terminale Myofibrillenabschnitt etwa die Länge einer Sarkomere, hat die Ribosomenkonzentration abgenommen, die Filamentanzahl zugenommen. In diesem Stadium treten zwischen den Aktinfilamenten erste Myosinfilamente auf. Eine neue Z-Scheibe entsteht in engem Kontakt mit dem Sarkolemm. Letztere löst sich nach und nach über ihre ganze Länge von der stets schräg ansetzenden Zellmembran. Die Frage nach der Spannungsübertragung an undifferenzierten terminalen Myofibrillenabschnitten wird diskutiert.
    Notes: Summary Longitudinal growth of myofibrils in the skeletal muscle of Macropodus opercularis appears to take place at their terminal parts. Since the Z-disks are arranged in lines and perpendicularly to the longitudinal axis, there are repeated terminal myofibril regions of different lengths following the last complete sarcomere. At the shortest terminal myofibril region, which is apparently the youngest one, ribosome concentration is very high. In the adjacent terminal regions of greater length, which probably represent older ones, actin filaments can be detected among the ribosomes. As soon as the terminal myofibril region approaches the full sarcomere length, the concentration of ribosomes is found to be reduced and the number of filaments increased. At this stage the first myosin filaments are clearly observed among actin filaments. Therefore, during longitudinal growth myosin filaments morphologically appear after the formation of actin filaments, whereas during myofibrillogenesis in the same muscle both types appear simultaneously in the differentiating sarcomere. After the arrangement of actin and myosin filaments into the A- and I-bands, a new Z-disk is formed in close contact with the sarcolemma and gradually detached over its entire length from the inclined cell membrane. The problem of tension transmission via the undifferentiated terminal myofibril regions is discussed in relation to these findings.
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    URL: http://dx.doi.org/10.1007/BF00306775
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  • 28
    Electronic Resource
    Electronic Resource
    Collar cells in planula and adult tentacle ectoderm of the solitary coral Balanophyllia regia (anthozoa eupsammiidae) (1973)
    Springer
    Cell & tissue research 145 (1973), S. 57-74 
    Details
    ISSN: 1432-0878
    Keywords: Collar cells ; Planula ; Larva, Balanophyllia regia (coral) ; Sense organs ; Evolution ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The planula larva of the solitary coral Balanophyllia regia has an ectoderm of flagellate, diplosomal collar cells. The collar of these cells is composed of a ring of microvilli linked with mucus strands. Four types of flagellate gland cells, three types of nematocyst and spirocysts are present in the planula ectoderm. The function of these ectoderm cells is discussed. The mesogloeal muscular and packing tissues of the planula are briefly described. The tentacle of the adult coral, examined for comparison, has an ectoderm of flattened flagellate cells with a shallow collar. Collar cells similar to those of the planula are occasionally found on the tentacle and their function is not known. Independent sensory cells built on a modified collar cell plan with collar of thickened microvilli are common in the tentacle. These are quite separate from the three kinds of tentacular nematocyte. Distended glandular areas occur in the tentacle ectoderm. The flagellate tentacle gastrodermis, muscle and mesogloeal region are briefly described. The evolutionary significance of collar cell ectoderm in a planula is discussed and the occurrence of collar cells throughout the animal kingdom, reviewed.
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    URL: http://dx.doi.org/10.1007/BF00307189
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  • 29
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    Electronic Resource
    Effects of calcitonin on osteoclasts in vivo (1973)
    Springer
    Cell & tissue research 145 (1973), S. 75-87 
    Details
    ISSN: 1432-0878
    Keywords: Osteoclasts ; Calcitonin ; Bone resorption ; Acid phosphatase ; Autophagocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Osteoclasts from the tibial metaphyses of young rats treated with porcine calcitonin were studied by electron microscopy. The animals were sacrificed 1 1/2, 4, 8 or 12 hours after injection of the hormone. In survey sections examined by light microscopy the osteoclasts appeared smaller than in control animals. At the ultrastructural level the osteoclasts showed the following alterations: 1) The typical ruffled border was absent. 2) Acid phosphatase was not present in the extracellular space between cell and bone. 3) The number of large vacuoles was decreased and there was no local accumulation of vacuoles in the cytoplasm. 4) The vacuoles did not contain bone crystals. 5) Vacuoles with cell organelles were increased in number. The majority of these vacuoles were identified as autolysosomes because they contained acid phosphatase and the enclosed cell organelles were partially digested. The above changes were present at all time intervals studied. The findings suggest that calcitonin decreases or inhibits bone resorption by osteoclasts. A decreased function of the osteoclasts may contribute to the hypocalcemic effect of the hormone. The increased number of autolysosomes is evidence of an enhanced autophagocytosis. Possible origins of the autolysosomes in osteoclasts are discussed.
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    URL: http://dx.doi.org/10.1007/BF00307190
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  • 30
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    Electronic Resource
    Histologische und quantitative Studien am lumbalen Glykogenkörper der Vögel (1973)
    Springer
    Cell & tissue research 145 (1973), S. 89-101 
    Details
    ISSN: 1432-0878
    Keywords: Glycogen body ; Avian lumbar spinal cord ; Aminergic innervation ; Drug treatment ; Glycogen bioassay ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Mit der Methode von Falck-Hillarp lassen sich im Rückenmark der Vögel Bündel fluoreszierender Nervenfasern nachweisen, die in Höhe des Glykogenkörpers (Lumbalwulst) seitlich an dieses Organ grenzen, von wo aus zahlreiche Fasern in den Glykogenkörper eindringen. Vergleichend-anatomisch gehören die fluoreszierenden Areale des Rückenmarks zu den autonomen Zentren. Es wurde versucht, mit Pharmaka, die das autonome Nervensystem beeinflussen oder Krämpfe erzeugen, das Glykogen des Lumbalwulstes zu mobilisieren (Quantitative Glykogenbestimmung nach Krisman, 1962). Besondere Beachtung verdient die elektronenmikroskopische Feinstruktur der Zwickelräume zwischen den großen Glykogenkörperzellen, wo Ausläufer dieser Zellen und Nervenfasern eng beieinander liegen. Die Kapillaren des Glykogenkörpers werden fast vollständig von Ausläufern seiner Zellen umscheidet.
    Notes: Summary With the method of Falck-Hillarp bundles of fluorescent nerve fibers were observed in the vicinity of the avian lumbar glycogen body. They encompass the glycogen body laterally. Numerous fluorescent fibers penetrate from this border zone into the glycogen. The fluorescent regions of the avian lumbar spinal cord belong to the autonomic centers. Attempts were made to decrease the high glycogen content of the glycogen body by administering drugs which act on the autonomic system, and also by using convulsant drugs. Glycogen was estimated quantitatively by the Krisman method (1962). Electron micrographs show that the triangular spaces between the enlarged cells of the glycogen body are occupied by the processes of these cells and by nerve fibers. The capillaries of the glycogen body are nearly completely ensheathed by the processes of the glycogen-body cells.
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    URL: http://dx.doi.org/10.1007/BF00307191
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  • 31
    Electronic Resource
    Electronic Resource
    On the fine structure of the Octopus iris (1973)
    Springer
    Cell & tissue research 145 (1973), S. 119-129 
    Details
    ISSN: 1432-0878
    Keywords: Iris ; Cephalopod (Octopus vulgaris.) ; Chromatophore ; Innervation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Octopus iris is composed of five different layers: A, the external epithelium; B, the chromatophore layer; C, the iridocyte layer; D, the layer of muscles and collagen strands; E, the pigment epithelium. The nerves innervating the sphincter and the chromatophore muscles are identified and their neuromuscular junction is described. The motor endings of chromatophore nerves have an additional ending in presynaptic position which probably functions as a modifier of neuromuscular transmission. The chromatophores are naked and exhibit a tubular channel system between plasmalemma and pigment container which looks similar to the T-system of muscle cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307193
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  • 32
    Electronic Resource
    Electronic Resource
    Effects of colchicine on myofilament arrangement and the lysosomal system in skeletal muscle (1973)
    Springer
    Cell & tissue research 144 (1973), S. 467-473 
    Details
    ISSN: 1432-0878
    Keywords: Muscle ; Colchicine ; Myofibrils ; Acid phosphatase ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Colchicine was intraperitoneally administered chronically to adult male Sprague-Dawley rats. The ultrastructural study of hind-limb muscles revealed that myofilament desorientation resulted. Bundles of myofilaments were found coursing perpendicular or oblique to the longitudinal axis of the muscle fiber. It is concluded that a colchicine-sensitive factor is involved in maintaining normal orientation of myofibrils in mature muscle. Also found in the sarcoplasm of the colchicine treated animals were complex spheromembranous bodies. These bodies enveloped mitochondria or other organelles and appeared to be derived from the sarcoplasmic reticulum. The lysosomal nature of these bodies is indicated by the localization of acid phosphatase activity in them. Acid phosphatase activity was also displayed in the sarcoplasmic reticulum. The spheromembranous bodies seem to be part of a sarcotubulo-lysosomal system in skeletal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307374
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  • 33
    Electronic Resource
    Electronic Resource
    Surface coat and endocytosis of specialized connective tissue cells in pulmonates (1973)
    Springer
    Cell & tissue research 146 (1973), S. 139-146 
    Details
    ISSN: 1432-0878
    Keywords: Amoebocytes ; Globular cells ; Pulmonates ; Cell coat ; Endocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cell coat of the amoebocytes and globular cells occurring in the connective tissue of Helix pomatia and Cepaea nemoralis was studied with colloidal iron, Thorotrast, ruthenium red and Alcian blue methods. The positive reactions of the entire cell surface of amoebocytes to these cationic dyes indicate the presence of an acid sialic-glycoprotein. The cell coat of the invaginations of globular cells proved to be distinct from the remainder cell surface since it has shown the strongest reaction of ruthenium red. The importance of the cell coat in the mechanism of endocytosis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306763
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  • 34
    Electronic Resource
    Electronic Resource
    Innervation of the testicular interstitial tissue in reptiles (1973)
    Springer
    Cell & tissue research 146 (1973), S. 123-138 
    Details
    ISSN: 1432-0878
    Keywords: Leydig cells ; Testis ; Innervation ; Reptiles ; Electron microscopy ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the tortoise Testudo graeca, the lizards Lacerta dugesi and Lacerta pityusensis, and the snake Natrix natrix, the innervation of the testicular interstitial tissue was studied by light and electron microscopy, the acetylcholinesterase (ache) technique, the Falck-Hillarp method for the detection of catecholamines, and the application of 6-hydroxydopamine. The intertubular spaces of the reptilian testes studied contain adrenergic nerve fibers the amount and distribution of which varies considerably both in various species and in various stages of the reproduction cycle. Nerve fibers do not enter the seminiferous epithelium. Fluorescence microscopy of the lizard testis reveals catecholaminergic varicosities which are mainly arranged around blood vessels, but do not show obvious connexions to Leydig cells. Ache-positive fibers are equally distributed in lizard testes surrounding each seminiferous tubule. In Natrix natrix ache-positive fibers are irregularly spread among groups of tubules, without showing a definite relation to Leydig cells either. By electron microscopy bundles of unmyelinated axons and axon terminals can be more easily detected in the testes of immature animals than in adult. Terminals of nerve fibers containing small (400–500 Å in diameter) and large (800–1400 Å) dense-cored vesicles and sometimes small clear vesicles establish contacts with Leydig cells. Three types of contact are described. 1. “Contacts” par distance at a distance of about 2000 Å and basal lamina interposed; 2. membranous contacts having a 200 Å gap only between axolemma and Leydig cell plasmalemma; 3. invaginations of terminals into Leydig cell perikarya. The latter may exhibit surface specialisations, which strongly resemble postsynaptic membrane thickenings. Experiments using 6-hydroxydopamine underline the adrenergic character of testicular nerve fibers, which can be regarded as another example of non-cholinergic, ache-positive neurons. In the testis of the immature tortoise profiles of axons occur which probably represent purinergic, ache-positive neurons.
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    URL: http://dx.doi.org/10.1007/BF00306762
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  • 35
    Electronic Resource
    Electronic Resource
    Die Feinstruktur des Protonephridialsystems von Turbanella cornuta Remane, einem marinen Gastrotrich der Ordnung Macrodasyoidea (1973)
    Springer
    Cell & tissue research 136 (1973), S. 277-289 
    Details
    ISSN: 1432-0878
    Keywords: Protonephridial system ; Turbanella cornuta Remane (Gastrotricha) ; Cyrtocytes ; Fine structure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Protonephridien des marinen Gastrotrichs Turbanella cornuta Remane werden elektronenmikroskopisch untersucht. Die Terminalapparate der Macrodasyoidea sind Cyrtocyten. Ein Reusenröhrchen besteht aus acht Längsstäben mit netzartigen Zwischenwänden aus dünnen Fibrillen. Im Lumen des Röhrchens schwingt eine Geißel. Die Wand eines Terminalbechers bildet ein zusätzliches Reusensystem, das mit Poren und Schlitzen versehen ist. Die drei bis vier Cyrtocyten eines Protonephridiums münden in eine Sammelzelle ein. Von dieser geht ein Exkretionsröhrchen mit Treibwimperflamme aus, welches von einer Ausleitungszelle gebildet wird. Die Cyrtocyten der beiden Gastrotrichenordnungen (Chaetonotoidea u. Macrodasyoidea) grenzen sich strukturell gegenüber bisher bekannten Formen dieses Zelltyps ab.
    Notes: Summary The protonephridial system of the marine gastrotrich Turbanella cornuta Remane was studied with the electron microscope. The terminal cells of the Macrodasyoidea are Cyrtocytes. Each tube for filtration consists of eight longitudinal rods with a net of fine fibrils between them; it contains a single whip. The wall of a terminal cup with its pores and slits is an additional system for filtration. Three or four filtration tubes are ending in a collecting cell. From this cell an excretory channel with one whip formed by an outlet cell arises. The filtration tubes of Macrodasyoidea are similar to those of Chaetonotoidea and differ from other forms of this cell-type.
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    URL: http://dx.doi.org/10.1007/BF00307445
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  • 36
    Electronic Resource
    Electronic Resource
    The fine structure of the pancreatic nerves of the domestic fowl (1973)
    Springer
    Cell & tissue research 136 (1973), S. 501-510 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas ; Fowl ; Innervation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the pancreatic nerves of the domestic fowl has been studied. Naked axon beadings were found in membranous contact with endocrine as well as exocrine cells. From an anatomical point of view it seems reasonable to suggest that the endocrine glands might be subjected to some influence of the autonomic nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307366
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  • 37
    Electronic Resource
    Electronic Resource
    Lymphoid stem cell identification in the developing thymus and bursa of fabricius of the chick (1973)
    Springer
    Cell & tissue research 136 (1973), S. 521-533 
    Details
    ISSN: 1432-0878
    Keywords: Thymus ; Bursa of Fabricius ; Chick embryo ; Lymphoid precursor cell, Lymphoid cell homing ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lymphoid precursor cell structure in the earliest stages of lymphoid development of the chick thymus and bursa of Fabricius was investigated light- and electron microscopically. The first lymphoid precursor cells around (8 days' incubation) and within (9 days') the epithelial thymus show the morphological features of motile cells, viz. pseudopodia and cytoplasmic bundles of filaments. Subsequent differentiation into lymphoid cells includes disappearance of pseudopodia and filaments, and appearance of a layer of marginal condensed heterochromatin in the nuclei. The first lymphoid precursor cells in the bursa mesenchyme (11 days' incubation) and bursa epithelial bud (12 days') are also characterized as motile cells; the intra-vascular lymphoid cells (13 days') of the bursa, though resembling the lymphoid cells in other localizations, lack their irregular cytoplasmic contours. The motile lymphoid cells in the early stages of lymphoid development of the chick thymus and bursa are not identical, but are distinguished by their cytoplasmic and nuclear organization: endoplasmic reticulum profiles and marginal heterochromatin, present in lymphoid cells of the bursa, are absent in those of the thymus. These and other data suggest that thymus and bursa lymphoid cells originate in different haemopoietic organs, those of the thymus in the yolk sac and those of the bursa in the spleen.
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    URL: http://dx.doi.org/10.1007/BF00307368
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  • 38
    Electronic Resource
    Electronic Resource
    Pinocytose und Bewegung von Amöben (1973)
    Springer
    Cell & tissue research 137 (1973), S. 97-109 
    Details
    ISSN: 1432-0878
    Keywords: Amoeba proteus ; Adsorptive function of mucoid layer ; Significance for induced endocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Mucoidschicht von Amoeba proteus enthält saure Mucopolysaccharide und ist funktionell einem Kationenaustauscher vergleichbar. Schwermetallpartikel und Proteine werden in Abhängigkeit vom pH-Wert des Kulturmediums in unterschiedlich starken Mengen an die Mucoidfilamente gebunden. Die dem Plasmalemm unmittelbar aufliegende globuläre Grundschicht besitzt dagegen für die untersuchten Substanzen keine Adsorptionsfunktion und unterscheidet sich demnach sowohl physiologisch als auch chemisch von der filamentartigen Zone. Quantitative Messungen über die pH-abhängige Anlagerung von Ferritin haben ergeben, daß die Mucoidfilamente in der Lage sind, Kationen aus dem Kulturmedium bis zu einer um das 17fache höheren Konzentration anzureichern. Die Ergebnisse der vorliegenden Untersuchung sprechen dafür, daß die Anreicherung derartiger Substanzen an der Zelloberfläche eine notwendige und physiologisch sinnvolle Voraussetzung für die Auslösung einer induzierten Endocytose darstellt.
    Notes: Summary The mucoid layer of Amoeba proteus contains acid mucopolysaccharides, which are involved in the exchange of cations. Depending on the pH of the external medium, different amounts of heavy metal particles and proteins are bound to the mucoid filaments. The globular ground layer, which is directly apposed to the plasma membrane does not bind any of the substances studied and, therefore, differs from the mucoid filaments in function as well as chemical nature. Measurements of the pH-dependent adsorption of ferritin demonstrate that the mucoid filaments are able to accumulate cations in concentrations 17 times that of the external medium. These results suggest that the accumulation of substances at the cell surface is a prerequisite for the initiation of induced endocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307051
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  • 39
    Electronic Resource
    Electronic Resource
    Ultrastructural observations on membranous structures in developing mouse oocytes (1973)
    Springer
    Cell & tissue research 137 (1973), S. 159-166 
    Details
    ISSN: 1432-0878
    Keywords: Ovum ; Mouse ; Membranes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies have revealed the presence of unusual membrane complexes within developing mouse oocytes. These structures, most obvious 18 days post fertilization, are found in the nucleus or cytoplasm of cells in meiotic prophase. The complexes, usually found in small groups, are characterized by a slightly bowed appearance, and a thin middle section that is vesiculated at each end. At high magnification the middle section exhibits a pentalaminar structure similar to tight junctional complexes, while the looped membranes of the vesiculated ends are trilaminar in appearance. In addition to being free in the nucleoplasm or cytoplasm, the complexes are also seen in continuity with the inner and outer leaflets of the nuclear envelope, and with typical membranes forming cytoplasmic tubular systems. The possible formation of these complexes from blebs or vesicles derived from the nuclear envelope is presented and the role that these structures may play in developing oocytes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307427
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  • 40
    Electronic Resource
    Electronic Resource
    Germ cell degeneration and intercellular bridges in the human fetal ovary (1973)
    Springer
    Cell & tissue research 138 (1973), S. 23-30 
    Details
    ISSN: 1432-0878
    Keywords: Germ cell ; Human degeneration ; Intercellular bridges ; Oogenesis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intercellular bridges between developing germ cells were observed in human fetal ovaries at 10 to 20 weeks gestation. Bridges were frequently found between cells in early stages of degeneration, with similar regressive changes being present in the conjoined cells. In advanced stages of cellular degeneration, bridges were less frequently found and were generally distorted and partially disrupted. Similarity in appearance of adjacent degenerating cells was common, even in late stages of degeneration. These observations suggest that cellular interconnection may be responsible for synchronous degeneration of germ cells during oogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307075
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  • 41
    Electronic Resource
    Electronic Resource
    The ultrastructure of dentinogenesis and amelogenesis in rat molar tooth germs grown as organ cultures in vitro (1973)
    Springer
    Cell & tissue research 138 (1973), S. 171-186 
    Details
    ISSN: 1432-0878
    Keywords: Tooth germs ; Organ culture ; Differentiation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tooth germs from foetal rats of 17 days post-insemination were maintained in vitro for 12 days. Odontoblasts and ameloblasts differentiated and secreted their respective matrices in which mineralization occurred. The ultrastructure of the cells was qualitatively similar to that observed in normal development. Odontoblasts contained more lysosome-like bodies and were found to degenerate in some sites. Mantle dentine was formed but few von Korff fibres were observed. Calcospherites were rarely seen and the mineralizing front of dentine was predominantly linear, associated with numerous small early foci of mineral formation. Enamel showed prism formation associated with the Tomes' process of the ameloblast but some local disturbances in the pattern of enamel formation were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306606
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  • 42
    Electronic Resource
    Electronic Resource
    A radioautographic study of the transport of 125I-labeled serum lipoproteins in rat aorta (1973)
    Springer
    Cell & tissue research 138 (1973), S. 223-237 
    Details
    ISSN: 1432-0878
    Keywords: Aorta, endothelium ; Rat ; Transport of serum lipoproteins ; Radioautography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The transport of 125I-labeled serum lipoproteins through the aortic endothelium was studied by radioautography. Rat aorta and heart was perfused in vitro with a medium containing human very low density (VLDL), low density (LDL), high density lipoprotein (HDL), delipidated HDL apolipoprotein or rat HDL. In all lipoproteins more than 95% of the radioactivity was TCA precipitable and lipid radioactivity was from 2–4% in HDL, 4–6% in LDL, 7–15% in VLDL. After 18–60 min of perfusion and wash with unlabeled medium, most of the aortic radioactivity was TCA precipitable and the percent of lipid counts was similar to that in the original lipoprotein. Following perfusion with VLDL, LDL, or HDL the radioautographic reaction was seen over the endothelium, the subendothelial space and the inner media, and was separated by an unlabeled zone from the reaction present over the adventitia. Uniform labeling of the entire wall was found after perfusion with HDL apolipoprotein. The presence of silver grains over endothelial cells in regions rich in plasmalemmal vesicles suggested that these organelles participate in the transport of the labeled lipoprotein, as was shown for lactoperoxidase (Stein and Stein, 1972). The present data indicate that cholesterol may enter the aortic wall as a constituent of lipoprotein particles. Since an HDL particle carries less than 1/20 of the cholesterol present in a LDL particle, it seems that the lower susceptibility of the female to atheromatosis might be related to the higher ratio of HDL to LDL particles in the female serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306609
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  • 43
    Electronic Resource
    Electronic Resource
    Über das fetale Gefäßsystem der reifen menschlichen Placenta (1973)
    Springer
    Cell & tissue research 139 (1973), S. 333-350 
    Details
    ISSN: 1432-0878
    Keywords: Placenta ; Human ; Blood vessels ; Endothelium ; Vascular muscle, Myoendothelial junctions ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Gefäße der Nabelschnur, Chorionplatte, Stammzotten und das Mikrozirkulationssystem des Zottenbaums der reifen menschlichen Plazenta wurden elektronenmikroskopisch untersucht. Die Endothel- und Muskelzellen besitzen auch am Ende der Gravidität alle morphologischen Kennzeichen voller Funktionsfähigkeit. Auffällig sind die zahlreichen myo-endothelialen Verzahnungen und die zahlreichen Kontakte der Muskelzellen untereinander. Sphinkterartige Einrichtungen werden nur an den Verzweigungen der Präkapillaren beobachtet. Gefäßnerven fehlen überall. Möglicherweise kann die Durchströmung der fetalen Placentagefäße durch autonome Kontraktion der Gefäßwände reguliert werden. — Alle Gefäße der Placenta sind elasticafrei. In den Nabelarterien sind elastische Systeme schwach ausgebildet. Eine Elastica interna wird nur in der Nabelvene gefunden. — Das Mikrozirkulationssystem in den Rami und Ramuli chorii sowie den Terminalzotten wird von allen bekannten Endstromeinheiten aufgebaut. Die englumigen Kapillarabschnitte befinden sich vor allem in unmittelbarer Nachbarschaft zum organellenreichen Syncytiophoblast, während die weitlumigen Abschnitte, die möglicherweise die venösen Kapillarstrecken sind, den Epithelplatten anliegen.
    Notes: Summary In the human placenta at term the blood vessels of the umbilical cord, chorionic plate and trunks and the microcirculation system of the placental lobes have been investigated. Also at the end of pregnancy the vascular endothelium and the smooth muscle cells exhibit all morphological criteria of full functional activity. The vessel wall is characterized by numerous myoendothelial junctions and many adjacent muscle cells being in close contact with one another. Sphincterlike structures can only be observed at the ramifications of the precapillaries. Vascular nerves are always absent. The blood circulation is perhaps regulated autonomously by means of the contractile vessel wall.—In all placental vessels a special tunica elastica never exists. In the umbilical arteries elastic systems are poorly developed. A genuine internal elastic layer only occurs in the corresponding vein.—In the region of the rami and ramuli chorii as well as in the single villi the microcirculation system consists of all types of terminal vessels. The capillaries with small diameter are preferentially situated close to the organellrich syncytiotrophoblast, whereas the distended segments, which may represent the venous capillaries, are in intimate contact with the epithelial plates.
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    URL: http://dx.doi.org/10.1007/BF00306590
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  • 44
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    Electronic Resource
    Zur Feinstruktur des dorsalen Riesenfasersystems im Bauchmark des Regenwurms (1973)
    Springer
    Cell & tissue research 139 (1973), S. 369-396 
    Details
    ISSN: 1432-0878
    Keywords: Synapses ; Giant fibres ; Ventral nerve cord ; Lumbricus terrestris L. ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die proximalen Kollateralen der dorsalen Riesenfasern des Regenwurms wurden in Serienschnitten vom Soma bis zum Eintritt in die Riesenfaser verfolgt und im Hinblick auf ihre Feinstruktur und ihre synaptischen Kontakte Untersucht. Es finden sich sowohl chemische als auch elektrische Synapsen. Ihre Feinstruktur wird mit der bekannter Synapsen anderer Wirbellosen und Wirbeltiere verglichen. In beiden Riesenfasersystemen kommen efferente chemische Synapsen mit feinen postsynaptischen Verzweigungen vor, die anscheinend von Bauchmark-Motoneuronen stammen. Das Axon der medianen Riesenfaser weist darüber hinaus nur noch eine elektrische Synapse mit den Rieseninterneuronen auf. Demgegenüber erhalten die Kollateralen der lateralen Riesenfasern zahlreiche Afferenzen, die zum Teil als sensorische Fasern der Epidermis, multisegmentale Fasern der Hauptfaserzüge und Rieseninterneurone identifiziert werden konnten. Weitere Afferenzen stammen vermutlich von unisegmentalen Interneuronen her. Beide lateralen Riesenzellaxone bilden außerdem miteinander eine elektrische Chiasma-Synapse mit besonderen Membraneinfaltungen.
    Notes: Summary The proximal collaterals of the dorsal giant fibres of the earthworm were traced through serial sections from the cell bodies to the giant axons. Their structure and synaptic connections were examined. There are chemical as well as electrical synapses. Their fine structure is compared to that of other known invertebrate and vertebrate synapses. Both giant fibre systems have efferent chemical connections with thin postsynaptic arborizations which probably belong to ventral cord motoneurons. Moreover the median giant axon is connected by an electrical synapse with the giant interneurons. The lateral giant collaterals on the contrary receive many afferences through chemical synapses which were partly identified as sensory fibers from the epidermis, multisegmental axons from the main fibre bundles or giant interneurones. Other afferences probably come from unisegmental interneurones. In addition both lateral giant axons form an electrical chiasma synapse with special membrane folds.
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    URL: http://dx.doi.org/10.1007/BF00306592
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  • 45
    Electronic Resource
    Electronic Resource
    L3, the 3rd 2nd order neuron of the 1st visual ganglion in the “neural superposition” eye of Musca domestica (1973)
    Springer
    Cell & tissue research 139 (1973), S. 397-403 
    Details
    ISSN: 1432-0878
    Keywords: Visual system ; Musca domestica ; Monopolar cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Nur drei von den fünf Monopolarzelltypen L1, L2, L3, die in jeder Cartridge der Lamina vorhanden sind, sind synaptisch mit den sechs Rezeptorendigungen (R1–R6) verbunden. Entsprechend der Verteilung ihrer Dendriten sind zwei von den drei Neuronen (L1 und L2) zur gesamten Länge der Endigungen verbunden, während das dritte Neuron (L3) Verbindungen nur in dem äußeren Zweidrittel der Lamina hat. Obwohl diese drei Zellen als Neurone 2. Ordnung des Neurosuperpositionsauges wirken könnten, deutet ihre anatomische Organisation auf funktionelle Unterschiede hin.
    Notes: Summary Only three of the five types of monopolar cells which are present in each cartridge of the lamina have synaptic connections with receptor endings (R1–R6). Due to the distribution of their dendrites two of these (L1 and L2) contact the whole length of the six receptor endings of their cartridge whereas the third type (L3) contacts only their outer 2/3rds. Although these three cells may function as the second order neurons of the neurosuperposition eye, their anatomical relationship imply functional differences between them.
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    URL: http://dx.doi.org/10.1007/BF00306593
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  • 46
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    Electronic Resource
    A quantitative electron microscopic analysis of myelination in the optic nerve of suckling rats treated with an inhibitor of cholesterol biosynthesis (1973)
    Springer
    Cell & tissue research 140 (1973), S. 9-23 
    Details
    ISSN: 1432-0878
    Keywords: Optic nerve ; Myelination ; Hypocholesteremic drug ; Exogenous and endogenous cholesterol ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of AY-9944, an inhibitory cholesterol biosynthesis, on the myelination of the optic nerve of rats was studied. Suckling rats were injected intraperitoneally with the drug every other day from birth, and were sacrificed at 10, 20 and 30 days of age together with littermate controls. The analysis is based on counting, at the electron-microscope level, the number of unmyelinated axons and the number of myelin lamellae surrounding each myelinating axon. The results indicate that a decrease in endogenous cholesterol by AY 9944, induced an overall retardation of the myelination process in the optic nerve: a larger proportion of myelinated axons and smaller number of myelin lamellae around the myelinating axons, when compared with the littermate controls, was observed. Exogenous cholesterol from the maternal milk did not compensate for a lack in endogenous cholesterol. Degenerating myelin sheaths were frequently seen in the experimental optic nerves at 20 and 30 days of age. Numerous membranous, intracytoplasmic drug-induced inclusions were found at all ages studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307056
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  • 47
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    Electronic Resource
    Ultrastructural studies of spermatogenesis in Drosophila melanogaster meigen (1973)
    Springer
    Cell & tissue research 140 (1973), S. 125-144 
    Details
    ISSN: 1432-0878
    Keywords: Spermatogenesis ; Meiosis ; Drosophila melanogaster ; Synaptinemal complex ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Meiosis in male Drosophila melanogaster was studied with the electron microscope. The number and the distribution of ring channels between individual members of a cluster of primary spermatocytes was determined from serial sections. The tubules with a diameter of 45 nm characteristic for meiotic prophase nuclei gave a chromatin type reaction, when treated with EDTA according to Bernhard's technique, thus suggesting that these tubules contain DNA. A material resembling central regions of synaptinemal complexes was observed in the nucleolus of primary spermatocytes during the downward movement in the testis. It is suggested that they represent unused precursors to central components of synaptinemal complexes, which are absent at pachytene of Drosophila males. Up to six tail fibers (kinetosomes + axonemes) were observed in every primary spermatocyte prior to meiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307062
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  • 48
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    Electronic Resource
    Über eine unbekannte Membrandifferenzierung in Zellen des Jacobsonschen Organs der Katze (1973)
    Springer
    Cell & tissue research 140 (1973), S. 583-586 
    Details
    ISSN: 1432-0878
    Keywords: Organon vomero-nasale (cat) ; Bright Cells ; Crystal-like differentiations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In bright columnar cells of the vomero-nasal organ of the cat an hitherto unknown hexagonal crystal-like membrane differentiation, connected with the endoplasmic reticulum, was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306681
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  • 49
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    Electronic Resource
    Electron microscopic identification of several types of endocrine cells in the bronchial epithelium of human foetuses (1973)
    Springer
    Cell & tissue research 141 (1973), S. 401-412 
    Details
    ISSN: 1432-0878
    Keywords: Pulmonary epithelium ; Human fetus ; Endocrine cells ; APUD cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic investigations of the pulmonary epithelium of human foetuses reveal the occurrence of cells exhibiting fine-structural characteristics of polypeptide hormone producing APUD cells. Three types of cells were identified mainly on basis of the morphology of their secretory granules. Cells of type 1 have the appearance of monoamine storing cells and the dense core of vesiculated granules of these cells are reactive to argentaffine reaction performed directly on ultra-thin sections. Cells of type 2 contain granules of uniform shape and size and of rather homogeneous appearance. Besides in larger bronchial tubules these cells are localized in the epithelium of developing alveoli. Cells of type 3 with large osmiophilic granules tightly bound by a membrane are few and scattered. These cells are observed in larger bronchial tubuli only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307413
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  • 50
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    Electronic Resource
    Structural and functional changes in the malpighian tubules of Carausius morosus during dehydration and starvation (1973)
    Springer
    Cell & tissue research 141 (1973), S. 479-492 
    Details
    ISSN: 1432-0878
    Keywords: Malpighian tubule ; Carausius morosus ; Water balance ; Diuretic hormone ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Carausius morosus starved and deprived of water lose about 30% of their body weight in 4 days, mainly due to water loss. Isolated inferior tubules from starved dehydrated insects secrete urine at 0.041 nl·mm−1·min−1 compared with 0.118 nl·mm−1·min−1 in those from fed hydrated insects. This difference is due partly to the level of a diuretic (and perhaps also an antidiuretic) hormone in the haemolymph acting directly on the urine-secreting mechanism and partly to changes in the intrinsic capacity of the tubule cells for urine secretion. This latter change is accompanied by structural changes in the tubules. During starvation and dehydration the lumen becomes packed with white granules, the height of the type 1 cells is reduced, their basal infoldings and brush border become shorter and their mitochondrial volume is reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307119
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  • 51
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    Electronic Resource
    New results on the ultrastructure of frog rod outer segments (1973)
    Springer
    Cell & tissue research 143 (1973), S. 45-52 
    Details
    ISSN: 1432-0878
    Keywords: Frog (Retina) ; Rod, Outer Segments ; Method of preparation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fibrils are demonstrated as an ultrastructural component in ultrathin sections of frog rod outer segments by a modified method of preparation which leads to partial contrast reversal. The relationship between these results and those obtained by conventional treatment leading to positive contrast, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307450
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  • 52
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    Electronic Resource
    The effect of chlorpromazine and tetracaine on the rapid axonal transport of neurosecretory material in the hypothalamo-neurohypophysial system of the rat (1973)
    Springer
    Cell & tissue research 143 (1973), S. 71-91 
    Details
    ISSN: 1432-0878
    Keywords: Axonal transport ; Neurosecretion ; Posterior pituitary ; Anesthetics ; Chlorpromazine ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of chlorpromazine (cpz) and tetracaine (tc) on the rapid axonal transport of neurosecretory material (NSM) in the hypothalamo-neurohypophysial system was investigated. Following subarachnoidal injection of these drugs, the incorporation of (35S) cysteine into proteins of the supraoptic nucleus was slightly depressed. The protein-bound radioactivity in the posterior pituitary was markedly lowered in experimental rats which indicates a partial blockage of the rapid axonal transport of NSM along the hypothalamoneurohypophysial tract. Both cpz and tc induced an increase in the number of mitochondria and profiles of granular endoplasmic reticulum. The axons in the infundibulum and neurohypophysis were enlarged by dammed organelles, indicating a blockage of axonal transport. There was an increased number of microvesicles, often arranged in a crystalloid pattern, in the terminals. The number and distribution of neurotubuli and neurofilaments were not changed. A possible stimulatory effect of cpz on the release of NSM from the neural lobe is assumed Possible mechanisms for the action of mitotic inhibitors, transquilizers and local anesthetics are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307452
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  • 53
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    Electronic Resource
    Evidence for the existence of serotonin-, dopamine-, and noradrenaline-containing neurons in the gut of Lampetra fluviatilis (1973)
    Springer
    Cell & tissue research 141 (1973), S. 33-54 
    Details
    ISSN: 1432-0878
    Keywords: Intestine, Lampetra fluviatilis ; Serotonin-, dopamine-, noradrenaline-containing neurons ; Identification by histochemistry ; Electron microscopy ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoamine-containing neurons in the gut of Lampetra fluviatilis are characterized by histochemical, electron microscopical and biochemical methods. Strongly yellow fluorescent, probably serotonin-containing intrinsic neurons are found along the entire length of the intestine. Their processes aggregate to form large bundles of mainly non-terminal axons, constituting a subepithelial fibre plexus. This subepithelial, ganglion cell comprising plexus is connected to a wide-meshed subserosal plexus which has ganglion cells of different size and few varicose, single axons. Intermingled with both plexus there occur — in the anterior and middle but not in the preanal portion of the lamprey intestine — scattered green fluorescent intrinsic perikarya, emanating faintly green fluorescent, poorly varicosed axons. The formaldehyde-induced neuronal fluorophores conform to serotonin (yellow fluorescent compound), noradrenaline, and dopamine (green fluorescent substance), as revealed in microspectrofluorimetric recordings. The electron microscopical analysis of the yellow fluorescent intrinsic neurons in the terminal hindgut shows nerve cell pericarya and axons equipped with a typical population of occasional small granular and many large granular vesicles (750–1600 Å). The number and opacity of cores of the small and the osmiophilia of the cores of the large granular vesicles are significantly increased following short-term treatment with 5,6-dihydroxytryptamine. Long-term treatment with 5,6- or 5,7-dihydroxytryptamine provokes severe signs of ultrastructure impairment and eventual degeneration in the supposed serotonin-containing axons, besides indications of piling-up of organelles in the non-terminal axons due to arrest of axonal transport. Chromatography of acid extracts from the lamprey intestine, gills and kidney reveals the presence of serotonin (besides another unidentified indoleamine) and dopamine and noradrenaline in the gut, but only dopamine in the brain. The detection of serotonin, noradrenaline and dopamine in the lamprey gut is confirmed by chemical determinations. The occurrence of intrinsic serotonin-, noradrenaline- and dopamine-containing neurons in the gut of Lampetra fluviatilis deviates from the established pattern of innervation of the vertebrate intestine and is considered to be a remnant of an autonomic innervation principle common in invertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307395
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  • 54
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    Electronic Resource
    Structural connections between dense core vesicles in the central nervous system of Anodonta cygnea L. (mollusca, eulamellibranchia) (1973)
    Springer
    Cell & tissue research 144 (1973), S. 75-88 
    Details
    ISSN: 1432-0878
    Keywords: Dense core vesicles ; Ultrastructure ; Mollusc ; Nervous system ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy of the cerebro-visceral connectives of Anodonta cygnea shows numerous long rows of dense core vesicles within the smaller axons of the connectives. These vesicles are often linked together by bridges of electron-dense material reinforced by one or more “arms”. The use of the tilting stage on the electron microscope, which allows the bridges to be examined from different angles, confirms the presence of these vesicular connections. Sub-cellular fractionation of the cerebro-visceral connectives was carried out to study the nature of these bridged dense core vesicles. In the negatively stained vesicle fraction two or more dense core vesicles are joined in a row by connections similar to the vesicular bridges of the intact connective. In the fixed vesicle fraction, many of the dense core vesicles are connected by bridges but long rows of bridged vesicles are not seen. The functional significance of the vesicular bridges is discussed. Preliminary findings of this work have been reported previously (McLaughlin and Howes, 1973).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306687
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  • 55
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    Electronic Resource
    Electron microscopical studies on the structure, phagocytic properties, and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig (1973)
    Springer
    Cell & tissue research 144 (1973), S. 247-297 
    Details
    ISSN: 1432-0878
    Keywords: Resident peritoneal macrophages ; Exudate peritoneal macrophages ; Phagocytosis ; Peroxidatic activity ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A comparative study on the fine structure, phagocytic properties, and peroxidatic activity of guinea pig resident peritoneal macrophages and the monocytes and macrophages present in experimentally induced sterile peritoneal exudates is reported. A number of fine-structural characteristics of the resident macrophages are described, including a peculiar configuration of the cell surface termed “loops”. The structure of these loops suggests the existence of a cell coat on the cytoplasmic side of the plasma membrane. The following observations were also made. Exudate monocytes differ from resident peritoneal macrophages mainly by the limitation of peroxidatic activity to cytoplasmic granules. The resident peritoneal macrophages have peroxidatic activity in the endoplasmic reticulum, the nuclear envelope, the annulate lamellae, and some of the cisternae of the Golgi apparatus. Macrophages derived from exudate monocytes do not show the same distribution of peroxidatic activity as the resident peritoneal macrophages. Both types of macrophages are highly active in the uptake of exogenous material. The functional significance of these observations is discussed and the conclusion is drawn that peritoneal exudates contain two different types of macrophages: resident macrophages and exudate macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307305
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  • 56
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    Electronic Resource
    Sarcomere formation in chick striated muscle (1973)
    Springer
    Cell & tissue research 145 (1973), S. 167-170 
    Details
    ISSN: 1432-0878
    Keywords: Striated muscle ; Chick ; Sarcomere formation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sarcomere assemblage in striated muscle of the early developing chick embryo was studied with the electron microscope. In myogenic chick somites, non-striated myofibrils are seen with the electron microscope, prior to striated ones. These crude myofibrils are traversed at regular periodic intervals by a tubular system which is associated with dense Z-line material shortly after its appearance. Longer sarcomeres as well as banding patterns similar to those found in mature striated muscle follow and possibly depend on prior Z-line formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307385
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  • 57
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    The effects of niamid and reserpine on the nerve endings of the pineal gland (1973)
    Springer
    Cell & tissue research 145 (1973), S. 151-166 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Kitten ; Nerve endings ; Influence of Reserpine, Niamid ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Kitten pineal glands were studied cytochemically under normal conditions, after reserpine injection, and after niamid administration. Adrenergic nerve elements were in perivascular spaces while cholinergic terminals were adjacent to pinealocytes, often times in synaptic contact. BA reactions are primarily in dotted vesicles of adrenergic terminals with some reaction in granular vesicles. Positive reaction occurs along neurotubules and membranous structures of adrenergig nerve fibers and terminals indicating membrane-bounded BA's. Niamid increased the number and density of dotted vesicles, and some granular vesicles are increased in density and size. Reserpine produced a loss reaction in dotted vesicles and a loss of vesicle matrix, producing elliptical vesicles. There is loss of reaction of the dotted vesicles, but occasionally, the positive granular reaction remains. Cholinergic terminals demonstrate no changes with either niamid or reserpine. These findings indicate BAs are stored in reserpine sensitive dotted vesicles and membraneous structures. The findings also show that the dotted vesicle matrix is reserpine sensitive and is necessary for storage of the BA's. Possibly biogenic amines cannot be stored or synthesized in terminals unless the matrix of the dotted vesicle is intact.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307384
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  • 58
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    The fine structure of the conducting system of the monkey heart (Macaca mulatta) (1973)
    Springer
    Cell & tissue research 145 (1973), S. 191-211 
    Details
    ISSN: 1432-0878
    Keywords: Heart ; Monkey (Macaca mulatta) ; Conducting system ; Atrium ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the sino-atrial (S-A) node of the monkey heart two types of muscle cells occur: 1. typical nodal cells which are the predominant cells and form the nodal fibers. 2. “Intercalated clear cells” with various diameters (4 to 12 μm) and containing poorly developed myofibrils, rich in glycogen and demonstrating poor staining properties. These latter cells are dispersed, few in number, and never form discrete fibers of themselves, but are intercalated between the cell rows of the typical nodal fibers. Such intercalated clear cells become more numerous at the periphery of the node. Interconnection between the S-A node and the conventional atrial muscle is established by a progressive transformation of nodal fibers into atrial fibers producing an intermediate (or junctional) type of fiber at the nodal periphery. However, in addition, few nodal fibers make direct contact with the atrial cardiocytes. Our light and EM studies have failed to prove the existence of truly specialized internodal pathways. Nevertheless intercalated clear cells, nodal-like cells, junctional or intermediate type of cells are relatively frequent in valvular regions (Thebesian, Eustachian, A-V, fossa ovalis) and less frequent in other regions of the atrial wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307388
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  • 59
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    Electronic Resource
    A comparison of the fine structure of thoracic and abdominal interganglionic connectives in the newly hatched and adult stick insect, Carausius morosus Br. (1973)
    Springer
    Cell & tissue research 145 (1973), S. 299-309 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Stick insect ; Blood-brain barrier ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fat body sheath which surrounds the ventral nerve cord of the adult stick insect, Carausius morosus, is absent in the hatchling. Since the haemolymph sodium ion compositions of the two stages are similar, it is suggested that the fat body sheath is not a site of a blood-brain barrier dealing with extra-axonal regulation of sodium. Similarities of ultrastructure in connectives of adults and hatchlings are demonstrated, and tight junctions are shown to occur in perineural cells of both stages. The possible significance of this to the blood-brain barrier of this insect is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307160
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  • 60
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    Electronic Resource
    On the impulse conducting system of the monkey heart (Macaca mulatta) (1973)
    Springer
    Cell & tissue research 145 (1973), S. 363-388 
    Details
    ISSN: 1432-0878
    Keywords: Heart ; Monkey ; A-V node and bundle ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The atrio-ventricular (A-V) node of the monkey heart is located in the focus of converging atrial muscle. Three main atrial muscle strands, coming from the atrio-ventricular ring, the dorsal wall of the atria, and the ventral part of the atrial septum, converge in the nodal region where they overlap and are interconnected. The junctional type of fibers establishing interconnection between the atrial muscle and the nodal tissue are not strictly localized at the periphery of the node, but may be traced further, along the A-V ring and coronary sinus. The A-V node consists of a loose peripheral and a compact distal part. In the former, typical nodal fibers were found, while the compact part shows an important individual variation in structure and cell-types. In some monkey hearts, the nodal fibers gradually become broader bundle fibers, while in other specimens the junctional fibers surround the compact part and than penetrate the nodal-His (N-H) region. These junctional fibers become nodal fibers or are in terminal contact with large clear cells up to 50 μ in diameter. Clear cells of various diameters are often intercalated between the cell rows of the nodal and His-bundle fibers and may form a distinct cellular gate between the node and the His-bundle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307163
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  • 61
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    Electronic Resource
    Cell junctions of the human enamel organ (1973)
    Springer
    Cell & tissue research 146 (1973), S. 69-81 
    Details
    ISSN: 1432-0878
    Keywords: Human enamel organ ; Ameloblasts ; Cell junctions ; Tooth development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nature and distribution of cell contacts have been examined in the human enamel organ in bell stage. The lateral cell surfaces of secretory ameloblasts are linked at their distal (apical) and proximal (basal) parts by junctional complexes consisting of tight junctions, large intermediate junctions (zonulae adherentes), occasional gap junctions and one or more series of desmosomes. Scattered desmosomes and large gap junctions link epithelial cells of the external enamel epithelium, stellate reticulum, stratum intermedium and internal enamel epithelium including secretory ameloblasts. Furthermore the above-mentioned layers are also linked together by desmosomes and gap junctions. With increasing maturation of the enamel organ an increase in size and number of gap junctions is observed. Some possible implications of the role of the different junctions are considered. The gap junctions probably participate in cell differentiation in the normal morphogenesis of the teeth as well as in metabolic and ionic coupling of the cells of the enamel organ. By means of tight junctions, adjacent secretory ameloblasts cooperate to form a physical barrier which might prevent the diffusion of some types of molecules or substances (e.g. secretory material distally and acid mucopolysaccharides proximally) through the interspaces between the cells. Adhering junctions might assist in regulation of the mechanical properties of the enamel organ as a whole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306759
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  • 62
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    Electronic Resource
    Electron microscopic studies on guinea pig rib cartilage (1973)
    Springer
    Cell & tissue research 146 (1973), S. 83-102 
    Details
    ISSN: 1432-0878
    Keywords: Cartilage (Guinea pig) ; Chondrocytes ; Proteoglycans ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The guinea-pig rib cartilage consists of chondrocytes dispersed in an intercellular substance composed of collagen fibrils, often characteristically cross-striated, and polygonal granules. Electron-dense membrane-bounded matrix vesicles are also observed intercellularly, especially in the central, partly calcified zone of the cartilage. With respect to their location in a cross-section of the rib, the chondrocytes differ in size, shape and intracellular fine structure. Thus, three separate types of cells are recognized. Peripheral chondrocytes have a flattened shape and are largely occupied by the nucleus. In the cytoplasm, the granular endoplasmic reticulum is the most extensive organelle. Intermediate chondrocytes are oval or round in shape. The endoplasmic reticulum and the Golgi complex are both prominent. Mitochondria and membrane-bounded cytoplasmic dense bodies are more numerous than in the peripheral cells. The ground cytoplasm often contains a few lipid droplets. In the central chondrocytes, such droplets sometimes fill the entire cytoplasm. Concomitantly, the nucleus is usually completely heterochromatic and the cells are therefore regarded as being metabolically inert. After preparations including ruthenium red staining en bloc, the general stainability of the chondrocytes is decreased. Intracellularly, positive ruthenium red staining of granular material within the Golgi vacuoles are to be observed. Extracellularly, the matrix granules are stained with this polyvalent, cationic dye. Extraction of the cartilage with 4 M guanidine-HCl removes all matrix granules and about 70% of the proteoglycans, measured as hexosamine, from the tissue. It is concluded that the matrix granules contain proteoglycan complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306760
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  • 63
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    Fine structure and innervation of the avian adrenal gland (1973)
    Springer
    Cell & tissue research 146 (1973), S. 385-402 
    Details
    ISSN: 1432-0878
    Keywords: Interrenal cells ; Avian adrenal gland ; Lipid exocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cords of interrenal-cells of birds resemble “tubes without lumina”, which are lined by columnar cells arranged in double rows. A subcapsular and an inner zone of the interrenal gland may be distinguished according to the structure of their mitochondria, the existence of smooth and rough surfaced endoplasmic reticulum, and the lipid contents. The cells of the inner zone are clearly polarized. The extrusion of lipid by exocytosis is discussed. It is often difficult to decide, whether ultrastructural details are species - specific or indicate a certain state of function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346230
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  • 64
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    Histochemical demonstration of Δ5-3 ß-OHD activity in the granulosa cells of ovarian follicles of immature and mature mice correlated with some ultrastructural observations (1973)
    Springer
    Cell & tissue research 136 (1973), S. 215-228 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (mouse) ; Granulosa cells ; Steroidogenesis ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A systematic study of Δ5-3β-hydroxysteroid dehydrogenase in the granulosa cells of immature and mature mice was made. The histochemical results were compared with the ultrastructural findings on the same cells in an attempt to determine whether the granulosa cells are capable of a steroidogenic role. In newborn and immature mice the granulosa cells of a great amount of follicles demonstrated a moderate or strong histochemical activity. In mature mice the granulosa cells demonstrated a weak or moderate activity normally only in preovulatory follicles and in some other atretic follicles. The granulosa cells of the normal developing follicles did not show such activity. In addition the histological control of numerous parallel sections demonstrated particularly in immature ovaries the presence of a great amount of atretic follicles. In the cytoplasm of the granulosa cells of the follicles in immature ovaries only clusters of lipid droplets with ribosomes were noted; while in the preovulatory follicles of mature animals there started to appear mitochondria with tubular cristae, smooth membranes of the endoplasmic reticulum and irregular lipid droplets. In the obviously atretic follicles several granulosa cells as well as theca interna cells showed numerous lipid droplets and ribosomes together with different degenerating organelles. The granulosa cells of the normal developing follicles showed a well developed Golgi complex, granular endoplasmic reticulum and free ribosomes. The histochemical and ultrastructural findings suggest a steroidogenic role of the granulosa cells only in the larger preovulatory follicles (probably related to an early luteinization of this layer) but this role was not demonstrated in the same cells in normal developing follicles. In addition, since an histochemical positivity was demonstrated also in the granulosa cells of some obviously atretic follicles, it is possible that many of the follicles having granulosa cells filled with lipid droplets and attached ribosomes and histochemically positive might be, in the immature ovaries, in a very precocious stage of atresia. It is to precise for these cells whether a cytoplasm with these two strictly correlated components (lipid droplets and attached ribosomes) and showing an histochemical positivity could carry-on all the biochemical steps involved in steroid biosynthesis or only has only a temporary capability to produce some precursors of steroids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307442
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  • 65
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    The effects of desiccation and starvation upon the weight, histology and ultrastructure of the reproductive tract of Biomphalaria glabrata, intermediate host of Schistosoma mansoni (1973)
    Springer
    Cell & tissue research 136 (1973), S. 229-262 
    Details
    ISSN: 1432-0878
    Keywords: Reproductive tract ; Biomphalaria glabrata ; Desiccation ; Starvation metabolism ; Weighing experiments ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of desiccation (36 weeks), starvation (12 weeks) and recovery (4 weeks following 16 weeks of desiccation and 2 weeks following 9 weeks of starvation) upon the accessory sex glands and some other body parts of Biomphalaria glabrata were studied by means of weighing experiments and histochemical and ultrastructural methods. The results of the weighing experiments show that the various parts of the reproductive tract are not protected from involution processes during the adverse conditions, as some parts (the albumen gland, spermduct and prostate gland) showed a more pronounced decrease in weight than other body parts. Measurements of the epithelial heights of the accessory sex glands and ultrastructural observations corroborate the results of the weighing experiments. During the adverse conditions the organelles involved in the formation of the secretion granules (granular endoplasmic reticulum and Golgi-apparatus) became involuted. The secretion granules within the cells of the albumen gland, spermduct and prostate gland were broken down by crinophagy. This latter process was less pronounced in the oviduct, muciparous gland, oöthecal gland, uterus and vagina. During the adverse conditions the lipoprotein producing cell types (mainly in the male part) remained active longer than the polysaccharide producing cell types (mainly in the female part). The significance of this observation is discussed. The amounts of glycogen present in most tissues of the reproductive tract and of the other investigated body parts (the foot, columellar muscle and vesicular connective tissue cells) were not apparently diminished, even when the secretory cells showed clear signs of involution, as a result of long periods of adverse conditions. Within the recovery periods 50% of the snails resumed egg production. During recovery the relative increases in weight of the accessory sex glands seemed to exceed that of other body parts, indicating that the restoration of the accessory sex glands is favoured. Histologically, reactivation of the accessory sex glands was apparent after 36–72 hours of recovery. At this time secretory material was again observed within the cisternae of the gland cell Golgi-bodies. Because reactivation is fairly rapid, a nervous or hormonal control of this process appears more likely than control via the general metabolism. The possible functions of various body organs in storing food reserve material is discussed, and it is concluded that the glycogen storing vesicular connective tissue cells (which occur throughout the whole body in the connective tissue) play a major role.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307443
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  • 66
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    Annular nucleolus in Leydig cells of mouse (1973)
    Springer
    Cell & tissue research 137 (1973), S. 167-175 
    Details
    ISSN: 1432-0878
    Keywords: Leydig cells ; Mouse ; Ring-shaped nucleolus ; Endoplasmic reticulum ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Many mature Leydig cells of adult mice contain annular nucleoli. In the cytoplasm of these cells, there is an abundance of agranular endoplasmic reticulum and its modifications, i.e. membranous whorls and double-walled tubules. By contrast, the cytoplasm of Leydig cells with compact nucleoli shows abundant free ribosomes and granular endoplasmic reticulum. It is assumed that the granular endoplasmic reticulum shifts to the agranular endoplasmic reticulum and its modifications during the differentiation of the Leydig cell. For the maturation of the Leydig cell the annular nucleoli may play an important role.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307428
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    Riesenmitochondrien im Übergangsepithel der Harnblase des Maulwurfs (1973)
    Springer
    Cell & tissue research 137 (1973), S. 421-434 
    Details
    ISSN: 1432-0878
    Keywords: Giant mitochondria ; Transitional epithelium ; Urinary bladder (mole) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Harnblasenepithel des Maulwurfs wurde elektronenmikroskopisch untersucht. Bei bestimmten Tieren finden sich in den Intermediärzellen „matrixreiche“ Riesenmitochondrien. Diese Riesenmitochondrien scheinen aus normalen Mitochondrien hervorzugehen und enthalten verschieden geformte Einschlüsse. Andere Organe und Zellen bei Maulwürfen mit Riesenmitochondrien in den Intermediärzellen des Harnblasenepithels weisen normale Mitochondrien auf. Es handelt sich demnach hier um eine sog. „Mitochondriose der Intermediärzellen“. Bedeutung, Bildung und Abbau der Riesenmitochondrien werden diskutiert.
    Notes: Summary Giant mitochondria containing an enormous matrix volume were found in the intermediary cells of the urinary bladder transitional epithelium of the mole. Regularly shaped mitochondria develop club-like protrusions forming giant mitochondria that contain several types of inclusions. Various other tissues were examined, but we could state that giant mitochondria exist only in the intermediary cells of the urinary bladder epithelium. Thus a peculiar “intermediary cell mitochondriosis” is described in this paper, not regularly occurring in the mole. The functional significance of the giant mitochondria is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307213
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  • 68
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    Mode de formation des lamelles annelées résultant de l'évolution, en condition anhormonale, des ovocytes de Nereis diversicolor (Annélide Polychète) (1973)
    Springer
    Cell & tissue research 137 (1973), S. 481-492 
    Details
    ISSN: 1432-0878
    Keywords: Annulate lamellae ; Annelidae ; Oogenesis ; Endocrinology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'évolution en l'absence d'hormone cérébrale provoque, dans les ovocytes de N. diversicolor, la formation de lamelles annelées cytoplasmiques (L.A.). Le processus débute par l'apparition de lames de réticulum à proximité du noyau (15 jours d'évolution à 15° C). Les lames se rapprochent ensuite du noyau et des pores apparaissent, situés le plus souvent face à ceux de l'enveloppe nucléaire (20ème jour). La différenciation des lames du réticulum en L.A. progresse ensuite depuis les éléments les plus proches du noyau vers les plus externes. Dans le cytoplasme des ovocytes ayant évolué à la température de 20° C, on assiste en outre à la formation d'empilements de citernes ergastoplasmiques. Ces citernes, non situées à proximité du noyau, subissent également une transformation progressive en L.A. L'étude cytochimique effectuée par digestion enzymatique a montré que la pepsine dégrade fortement le matériel dense des lamelles annelées. Ce matériel semble donc renfermer une teneur élevée en protéines auxquelles pourrait se trouver associé du RNA.
    Notes: Summary Experimental elimination of brain activity leads to the formation of cytoplasmic annulate lamellae (L.A.) in oocytes of N. diversicolor. This process begins with the appearance of cisternae of endoplasmic reticulum in the vicinity of the nucleus (15 days of development at 15° C). Then, the cisternae move closer to the nucleus and pores appear, often facing the pores of the nuclear envelope (20th day). The differentiation of endoplasmic reticulum into L.A. progresses from the elements closest to the nucleus to the more peripheral ones. In the cytoplasm of oocytes which developed at 20° C, stacks of cisternae of rough endoplasmic reticulum have been observed. These cisternae, which are not localized near the nucleus, also undergo a progressive transformation into annulate lamellae. A cytochemical study, based on enzymatic digestion, shows that pepsin strongly digests the dense material of L.A. This material, therefore, seems to contain a high amount of proteins, perhaps associated with RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307225
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  • 69
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    Elektronenmikroskopische Untersuchungen an der Froschlunge (1973)
    Springer
    Cell & tissue research 137 (1973), S. 553-561 
    Details
    ISSN: 1432-0878
    Keywords: Lung (frog) ; Alveolar epithelium ; Cytosomes ; Alveolar lining layer surfactant ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Alveolarepithel der Froschlunge weist nur einen einzigen Zelltyp auf. Die Zellkörper sitzen in den Nischen zwischen den Kapillaren, die sie mit Zytoplasmaausläufern überdecken. Die Epithelzellen enthalten große Zytosomen mit osmiophilen Lamellen mit einer Periode von 40–42 Å. Sie sind den Typ II-Pneumozyten der Säugerlunge vergleichbar. Das Alveolarepithel der Froschlunge ist mit einer Grenzschicht bedeckt, die in Abhängigkeit von der Fixierung eine 40–42 Å-Periode aufweist oder aus einer oder mehreren Doppelmembranen zusammengesetzt ist. Gittermuster und Myelinfiguren sind vorhanden. Das bedeutet, daß Surfactant in der Froschlunge in gleicher Weise wie in der Säugerlunge dargestellt werden kann.
    Notes: Summary The alveolar epithelium of the frog exhibits only one type of cells. The cell-bodies are situated in the spaces among the capillaries, which they cover with cytoplasmic extensions. The epithelial cells contain large bodies (cytosomes) with osmiophilic lamellae having a period of 40–42 Å. The alveolar cells are considered to be similar to the type II-pneumocytes of mammalian lungs. The alveolar epithelium of the lung of the frog is covered with a lining layer, which depending on the method of fixation consists of periods of 40–42 Å or of one or more double membranes. Lattice formations and myelin figures are seen. This means that the surfactant in the lung of the frog can be demonstrated in the same way as in mammalian lungs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307230
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  • 70
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    On the axonal migration of catecholamines in constricted sciatic nerve of the rat (1973)
    Springer
    Cell & tissue research 138 (1973), S. 345-370 
    Details
    ISSN: 1432-0878
    Keywords: Axonal migration ; Catecholamines ; Sciatic nerve ; Radioautography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The radioautographic technique has been used to study the axonal migration of catecholamines in sympathetic fibres of the sciatic nerve of rats after ligature. A first series of experiments aimed at ascertaining the capacity of the proximal portion of adrenergic fibres to take up and store exogenous tritiated catecholamines (3H-DOPA; 3H-DA and 3H-NA) 3 to 22 hours after ligation. The results are qualitatively similar in rats pretreated and non-pretreated with IMAO, but the intensity of the radioautographic reaction is lower in the latter cases. Most of the labeled axons are filled mainly with vesicular and tubular profiles of endoplasmic reticular origin, large dense bodies (probably lysosomes) and/or mitochondria. The silver grains are generally superimposed on the vesicular and/or the tubular profiles, but in some cases on the large dense bodies, suggesting that exogenous catecholamines can be stored in lysosomes. The question whether SGV specifically store catecholamines also in the modified adrenergic fibres has been investigated in KMnO4 fixed material. These results show that beside a large number of fibres in which there is a strict correlation between labeling and SGV, some fibres containing SGV do not retain the 3H-NA. Conversely some fibres which contain mainly agranular vesicles display radioautographic reaction. Therefore, in case of ligated fibres, SGV cannot be considered the specific organelles for storage of catecholamines. The axonal migration of labeled catecholamines has been studied in animals pretreated with IMAO. A moderate, but selective, labeling is present in the proximal portion of sciatic fibres of rats in which administration of labeled catecholamine preceeded of 2 hours the ligature and this was performed 22 hours before fixation. From these combined types of experiments, it is concluded that despite the presence of all the structures necessary for the storage of a high amount of catecholamines in the modified adrenergic fibres, only a small fraction of catecholamines accumulated above the ligature has been transported by axonal migration. Therefore, the axonal migration of catecholamines appears as an epiphenomenon related to the distal migration of enzymatic and storage proteins from the perikaryon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307098
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  • 71
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    Ultrastructural changes in the coronet cells of the saccus vasculosus from rainbow trout, Salmo gairdneri (Richardson), kept in sea water (1973)
    Springer
    Cell & tissue research 139 (1973), S. 271-284 
    Details
    ISSN: 1432-0878
    Keywords: Saccus vasculosus ; Rainbow trout ; Coronet cell ; Sea water adaptation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The coronet cells of saccus vasculosus of fresh-water living and sea-water adapted rainbow trout were studied with the electron microscope, with special regard to changes in the latter group. Only quantitative differences were observed, namely a raised number of mitochondria in the apical region and the head and also a concentration of the agranular endoplasmic reticulum with a higher amount of electron-dense material and vesicles around the Golgi saccules. Together, these findings suggest a secretory function for the coronet cell. A supposed transport of vesicles from the head region of the coronet cell out into the globules is suggested. Interrelation between primary and secondary vesicles is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306526
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  • 72
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    The development of synapses in vitro between previously dissociated chick spinal cord neurons (1973)
    Springer
    Cell & tissue research 140 (1973), S. 203-216 
    Details
    ISSN: 1432-0878
    Keywords: Synapses ; Development ; Chick embryo ; Cell culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The formation and development of synaptic contacts between dissociated chick spinal cord neurons has been investigated. By the 6th day in vitro “immature” profiles with few vesicles were observed. By 14–18 days “mature” types with numerous vesicles were found, indistinguishable from those of newly hatched chick spinal cord. After this period degeneration occurred, and was especially marked in the post-synaptic element. Such degeneration could be postponed by the addition of small numbers of somatic muscle cells. The Kanaseki and Kadota (1969) technique was applied to the study of coated vesicles at various stages of synaptic development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306695
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  • 73
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    Quantitative microscopical studies of the mouse peritoneal macrophage following stimulation in vivo (1973)
    Springer
    Cell & tissue research 140 (1973), S. 187-202 
    Details
    ISSN: 1432-0878
    Keywords: Macrophage ; Differentiation ; Triolein ; Stereology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The results of an objective two- and three-dimensional analysis of the morphological features of normal and triolein-induced mouse peritoneal macrophages are reported. An “equivalent circle“ technique for resolving the effects of volume and surface area on volume-to-surface parameters is described. The method is a simple comparative one which does not require the actual determination of cell volume. Macrophage stimulation promoted increases in mean cell size, cytoplasmic granularity and volume-to-surface ratio. In addition, a reduction in nuclear volume-to-surface ratio accompanied in vivo stimulation. Nucleocytoplasmic ratio remained constant. The equivalent circle procedure showed that the increase in cellular volume-to-surface ratio was due largely to the increase in cell volume; the decrease in nuclear volume-to-surface ratio was primarily the result of a substantial increase in nuclear membrane surface area. Stereological estimations suggest that interiorized cell membrane (in the form of triolein-containing phagosomes) is replaced by newly reconstructed surface membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306694
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  • 74
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    Cytologische Studien an Trichocysten (1973)
    Springer
    Cell & tissue research 140 (1973), S. 235-259 
    Details
    ISSN: 1432-0878
    Keywords: Ciliated protozoa ; Loxophyllum and Prorodon ; Trichocysts ; Toxicysts ; Expulsion mechanism ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Nesselkapseltrichocysten der Ciliaten Loxophyllum und Prorodon wurden hinsichtlich ihrer Feinstruktur sowie des Ausschleuderungsablaufes untersucht. Loxophyllum besitzt zwei morphologisch unterscheidbare Sorten von Toxicysten, wohingegen Prorodon nur eine Art dieser Organelle aufweist (Krüger, 1936). Wie an Hand gehemmter Stadien gezeigt werden kann, verläuft die Ausschleuderung bis auf geringfügige Variationen bei den drei Sorten in der gleichen Art: Schläuche, die im ruhenden Zustand bereits in den endgültigen Dimensionen in der Toxicystenkapsel eng ineinandergeschachtelt vorliegen, werden handschuhfingerförmig umgestülpt und verlassen hierdurch die Kapsel und damit den Zellkörper. Dieser Prozeß ist mit der Ausscheidung einer teils fädigen, teils amorphen Substanz gekoppelt. Der Umstülpungsvorgang der Innenstruktur der Nesselkapseltrichocysten wird mit den völlig andersartigen, während der Ausschleuderung von Mucocysten und Spindeltrichocysten ablaufenden morphologischen Veränderungen verglichen. Es zeigte sich, daß aufgrund ihrer differierenden Feinstrukturen und Funktionsweisen in den Nesselkapseltrichocysten einerseits und in den Mucocysten sowie Spindeltrichocysten andererseits zwei grundsätzlich voneinander verschiedene Organellentypen gesehen werden müssen.
    Notes: Summary The toxicysts of the ciliates Loxophyllum meleagris and Prorodon teres were examined with regard to fine structure and expulsion mechanism. Loxophyllum possesses two morphologically distinct types of toxicysts, whereas in Prorodon only one type is present. As can be shown by discharge inhibition experiments, the expulsion mechanisms, except for small variations, are identical in all three types: Tubes, which in their resting state lie closely packed one within the other possess already at this state their final dimensions; they are inverted, thereby leaving the capsule and thus the cell body. This process is correlated with the excretion of a substance partly filamentous, partly amorphous. The inversion of the tubes during the expulsion is compared to the fundamentally different morphological alterations during discharge of mucocysts and spindle trichocysts. The differences and similarities in fine structure and function between toxicysts on the one hand and mucocysts and spindle trichocysts on the other, indicate that two rather than three fundamentally different organelle types must be distinguished.
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    URL: http://dx.doi.org/10.1007/BF00306697
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    Die Feinstruktur der Kehlkopfmuskeln des Laubfrosches, Hyla arborea arborea (L.), im Vergleich zu einem Skelettmuskel (1973)
    Springer
    Cell & tissue research 141 (1973), S. 223-233 
    Details
    ISSN: 1432-0878
    Keywords: Laryngeal muscles ; Tree Frog, Hyla aborea arborea (L.) ; Fine structure ; Motor endplates ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Um Hinweise auf die Funktion des Kehlkopfes bei der Rufabgabe zu bekommen, führten wir eine vergleichende elektronenmikroskopische Untersuchung an den vier Kehlkopfmuskeln rufaktiver Laubfrösche durch. Zum weiteren Vergleich studierten wir auch einen Extremitätenmuskel. Die Kehlkopfmuskeln sind untereinander gleich gebaut, unterscheiden sich aber in mehrfacher Hinsicht vom Extremitätenmuskel: Ihre Myofibrillen sind nur ein Drittel so dick. Die Kehlkopfmuskeln enthalten sehr viele, auffallend große und dichte Mitochondrien; das sarcoplasmatische Retikulum ist mächtig entwickelt. Sie enthalten außerdem sehr viel Fett, das stets mit den Mitochondrien assoziiert ist. In beiden Muskeltypen kommt Glykogen vor; seine Menge ist in den Kehlkopfmuskeln jedoch größer als im Extremitätenmuskel. Die Zahl der motorischen Endplatten ist in den Kehlkopfmuskeln 25 mal höher als im M. plantaris longus. Die Zahl der Triaden pro Sarcomer und ihre Anordnung ist bei den beiden Muskeltypen gleich. Die Kehlkopfmuskeln winterstarrer Hyla-Männchen unterscheiden sich in ihrem Feinbau und Fettgehalt nicht von den Muskeln rufaktiver Tiere.
    Notes: Summary In order to obtain information on the function of the larynx during vocalization, a comparative electron microscopic study was made of the four laryngeal muscles of male tree frogs druing their vocalizing phase. A skeletal muscle (m. plantaris longus) was examined for comparison. The fine structure of the laryngeal muscles is uniform, but differs from skeletal muscles in several respects: the diameter of the myofibrils of the laryngeal muscles is only 1/3 that of the skeletal muscle. They contain many strikingly large and dense mitochondria. There is a strongly developed sarcoplasmic reticulum. Laryngeal muscles contain large quantities of fat which is always associated with mitochondria. Laryngeal muscles contain more glycogen than skeletal muscle. The number of motor endplates in laryngeal muscles is 25 times larger than in m. plantaris longus. The number of triads per sarcomere and their location is the same in both types of muscle. The laryngeal muscles of hibernating and active tree frogs do not show any difference with respect to fine structure and amount of fat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00311355
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    Age changes in the centrally and peripherally located sensory neurons in rat (1973)
    Springer
    Cell & tissue research 141 (1973), S. 285-298 
    Details
    ISSN: 1432-0878
    Keywords: Sensory neurons ; Lipofuscin pigment ; Mitochondrion ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lipofuscin pigment formation and distribution in the Mes. N.5 neurons, trigeminal and spinal ganglia of male Wistar rats of 2, 14, 32 and 49 months as an indication of aging has been investigated. These intraneuronal pigment granules are found as early as 2 months in all the cells, and continue to accumulate in all the cells in varying amounts until the first year of life. The different rate at which lipofuscin accumulates probably shows the difference in the maturation of the functionally related cells. At later stages the obvious findings are complex pigment body formation and localization of the pigment bodies either at one pole as seen in the Mes. N. 5 neurons or arranged submembranously parallel to the long axis of the cells in the ganglia. The vacuolated lipofuscin pigment bodies are bound by a double limiting membrane and among the vacuoles are found tubular membranous structures resembling residual mitochondrial substructures. These findings suggest a mitochondrial origin of lipofuscin, rather than a lysosomal. The intracellular pigment bodies seen in the perineuronal satellite cells of peripheral ganglia appear to be signs of removal of lipofuscin from the ganglion cells.
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    URL: http://dx.doi.org/10.1007/BF00311358
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  • 77
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    Histological and ultrastructural observations on wound healing in the freshwater pulmonate Lymnaea stagnalis (1973)
    Springer
    Cell & tissue research 141 (1973), S. 561-573 
    Details
    ISSN: 1432-0878
    Keywords: Wound healing ; Lymnaea stagnalis ; Amoebocytes ; Phagocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The process of wound healing in Lymnaea stagnalis was studied by light and electron microscopy. Snails were wounded by making incisions in the skin. The observations showed that the wounds are closed by muscular contraction and by formation of thrombi of blood amoebocytes. These thrombi form a large amoebocyte plug. During the first 72 hrs after incision thin tubules (diameter 175–225 Å) were observed between the amoebocytes in the plug. Possibly these tubules represent a blood clotting protein. The round amoebocytes constituting the plug can be regarded as normal blood amoebocytes. First, ultrastructurally they closely resemble the amoebocytes of the circulating blood. Second, not only blood amoebocytes but also plug amoebocytes of snails injected with India ink before incision contained ink particles, indicating that the cells are of one type. Apparently due to phagocytosis of cell debris the number of lysosomes in plug amoebocytes increased during the first days after incision. Eighteen to twenty four hrs after incision the first signs of differentiation of round plug amoebocytes into flattened cells were observed. Between these cells collagen was seen from 3–5 days after incision and onwards. It is suggested that these flattened amoebocytes produce collagen fibrils. These cells are structurally different from collagen producing fibroblasts and from muscle cells of the surrounding connective tissue. Transformations of amoebocytes into these two latter cell types were not found. Ninety days after incision the connective tissue in the wound area is still different from that on non-injured sites.
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    URL: http://dx.doi.org/10.1007/BF00307125
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    Effects of parathyroid hormone on osteoclasts in vivo (1973)
    Springer
    Cell & tissue research 141 (1973), S. 529-544 
    Details
    ISSN: 1432-0878
    Keywords: Osteoclast ; Parathyroid hormone ; Lysosomes ; Bone resorption ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Young rats were treated with high doses of parathyroid hormone (PTH). Osteoclasts from these animals revealed characteristic alterations in comparison to control cells: a) The cytoplasm contained large vacuoles with phagocytosed cells, some of which resembled osteoblasts or osteocytes. The vacuoles were interpreted as lysosomes because the engulfed cells often appeared partly digested and the vacuoles contained acid phosphatase as demonstrated histochemically, b) lipid droplets were present in the cytoplasm and usually located close to the endoplasmic reticulum and/or in regions with many free ribosomes, c) the Golgi complex was more frequently separated from the nuclei than in control cells, d) small coated cytoplasmic bodies were numerous in the peripheral cytoplasm, e) the membranes of the endoplasmic reticulum were fused in some places, f) cytoplasmic regions with numerous free ribosomes were frequent, g) large ring-shaped granules occurred in some mitochondria. Energy dispersive X-ray analysis of these granules provided evidence that they contained calcium and probably phosphorus, h) in some osteoclasts the mitochondria were enlarged. — The findings are consistent with an increased activity of osteoclasts and in particular a stimulation of the lysosomal system in these cells.
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    URL: http://dx.doi.org/10.1007/BF00307123
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  • 79
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    Post-coital depletion of dense-core vesicles from the external layer of the rabbit median eminence (1973)
    Springer
    Cell & tissue research 142 (1973), S. 27-36 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (Rabbit) ; Dense-core vesicles depletion ; Releasing factors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of mating on the depletion of osmiophilic material from dense-core vesicles (DCV) within the external layer of the rabbit median eminence (ME) were investigated. Two different populations of DCV were demonstrated within the ME external layer of the non-mated control animals. A plot of the diameters of these vesicles showed two peaks, one at 90–100 nm, and the other at 120–130 nm. These two populations of DCV were found in separate axons and axon terminals in contact with, or near the perivascular spaces of, portal capillaries. Within these axons and terminals an occasional membrane profile or “vesicle ghost” was also observed. The same two populations of DCV were evident in the ME external layer of animals that were sacrificed at 10 minutes post-coitus. In these experimental animals, the number and size of the smaller population of DCV were the same as those of the none-mated controls. However, there was an obvious decrease in the number of the large (120–130nm) DCV, together with a simultaneous marked increase in the number of “vesicle ghosts”. These ghosts had a mean diameter of 137±14 nm. On the basis of their size, it is suggested that the “vesicle ghosts” represent large DCV that have been depleted of their content following mating. Ultrastructural evidence for the depletion of osmiophilic material from the large DCV of mated animals is provided. According to previous writers the smaller DCV within the ME are aminergic, whereas the larger DCV may contain releasing factors (RF). Our findings provide further morphological evidence to support this hypothesis. Furthermore, RF and biogenic amines appear to be contained within separate nerve terminals in the rabbit ME.
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    URL: http://dx.doi.org/10.1007/BF00306702
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    Topochemischer Nachweis von carbonylhaltigen Verbindungen im Thymus (1973)
    Springer
    Cell & tissue research 142 (1973), S. 465-477 
    Details
    ISSN: 1432-0878
    Keywords: Rabbit thymus ; Ketosteroids ; Granulated cells ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Topochemisch konnten im Kaninchenthymus Ketosteroide nachgewiesen werden, für deren Vorhandensein die chemische Analyse von Thymuslipidextrakten Hinweise ergeben hatte. Die Darstellung der Ketosteroide erfolgte mit der NAHD-Reaktion (Camber, 1949). Diese Befunde dürften als spezifisch gelten, da eine Verfälschung durch freie Gewebsaldehyde, Plasmalogene (Gomori, 1952) und Corticosteroide (Khanolkar et al., 1958) ausgeschlossen wurde. Mit Hilfe der Camber-Methode konnten Granula bestimmter Zellen des Kaninchenthymus selektiv dargestellt werden, die auf Grund morphologischer Kriterien in zwei Gruppen unterteilt wurden. Die Zellen liegen im gesamten Thymusparenchym sowie im Bindegewebe der Septen und der sogenannten „Kapsel“. Prädilektionsorte stellen die Umgebung der Hassallschen Körperchen, die Mark-Rinden-Grenze, der intra- und perivasale Raum sowie die subkapsuläre Zone dar. Beide Zellgruppen sind Sudan III-, PAS- und Eosin-positiv, besitzen eine ausgeprägte gelbgrüne Eigenfluoreszenz und können differentialdiagnostisch gegen Mastzellen abgegrenzt werden. Elektronenmikroskopisch zeigen beide Zellgruppen zahlreiche Übereinstimmungen mit Reifestadien eosinophiler Knochenmarkszellen.
    Notes: Summary Ketosteroids were demonstrated topochemically in the rabbit thymus following indications given by chemical analysis of thymus lipid extracts. They were revealed by means of the NAHD-reaction (Camber, 1949). The results are thought to be specific, because adulteration by free tissue aldehydes, plasmalogens (Gomori, 1952) and corticosteroids (Khanolkar et al., 1958) can be excluded. The Camber-method selectively reveals the presence of two groups of morphologically differentiable granules in specific rabbit thymus cells which are distributed throughout the entire thymic parenchyme as well as in the connective tissue of the septa and the in so-called “capsule”. They are mainly seen in the vicinity of Hassall's bodies, in the zone between medulla and cortex, the intra- and perivascular space and in the subcapsular space. Both cell groups are Sudan III-, PAS- and eosin-positive, show intensive yellow-green primary fluorescence and can be distinguished from mast cells by differential diagnosis. Electron microscopy reveals that both cell groups show many similarities with the maturation phase of bone marrow eosinophils.
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    URL: http://dx.doi.org/10.1007/BF00306710
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    Effects of colchicine on release of neurosecretory material from the posterior pituitary gland of the rat (1973)
    Springer
    Cell & tissue research 142 (1973), S. 443-464 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Posterior Pituitary gland ; Colchicine ; Axoplasmic flow ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of colchicine on the release of neurosecretory material from the posterior pituitary gland was investigated in the rat in vivo and in vitro. Colchicine was administered subarachnoidally when neurophysin, radiolabelled by injection of (35S) cysteine into the supraoptic nucleus, had accumulated in the neural lobe. Dehydration for 3 days of non-colchicine-treated rats was followed by a 100% reduction of neurophysin-bound radioactivity. When colchicine was given prior to dehydration, the reduction of radioactive neurophysin was less marked. Colchicine treatment alone was likewise followed by a lowering of protein-bound radioactivity in the neural lobe, which may indicate that colchicine, in addition to blocking the rapid axonal transport of neurosecretory material, also impedes the slow transport. The release of radioactive neurophysin in response to depolarizing concentration of potassium in vitro was diminished in the presence of colchicine, the reduction being most pronounced after colchicine treatment in vivo. The biochemical data prove the view that colchicine inhibits the release of neurosecretory material from the neural lobe. The ultrastructural findings support the biochemical data. Thus, colchicine treatment alone or followed by dehydration induced a marked increase in the number of organelles, especially of mitochondria and dense bodies. There was a marked increase in the number of enlarged axons filled with dammed organelles in the infundibulum and neurohypophysis. There was an accumulation of dense core vesicles and microvesicles in the axonal terminals in the neurohypophysis after treatment with either colchicine or colchicine followed by dehydration, which indicates an impediment of the release process. Dehydration alone induced a depletion of the dense core vesicles in the terminals. Out from the combined biochemical and ultrastructural findings possible mechanisms for the action of colchicine are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306709
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    Identification of adenohypophysiotropic neurohormone producing neurosecretory cells in Rana temporaria (1973)
    Springer
    Cell & tissue research 142 (1973), S. 479-513 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (Frog) ; Tubero-hypophysial system ; Neurosecretory axon types ; Release phenomena ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By means of electron microscopy, in the median eminence of Rana temporaria, the terminal arborizations of axons of six different types of neurosecretory cells, located in the pars ventralis of the tuber cinereum, were identified. In addition, phenomena connected with the release of neurosecretory material from the axon terminals of these neurosecretory cells into the blood capillaries of the median eminence are described. Preliminary results suggested the existence, in the median eminence, of additional different neurosecretory axon types which could also belong to corresponding neurosecretory cell types probably located in the apical part of the pars ventralis of the tuber cinereum. Moreover, in the external region of the normal median eminence, separate monoaminergic nerve fibres were tentatively identified. Arguments are adduced which plead (1) against the assumption that the ependyma or the pituicytes of the median eminence could produce adenohypophysiotropic hormones; (2) against the inference that the ependymal cells of the median eminence might be involved in the transport of adenohypophysiotropic hormones from the cerebrospinal fluid into the blood capillaries of the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306711
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    Ultrastructure of possible sites of ultrafiltration in some gastropoda, with particular reference to the auricle of the freshwater prosobranch Viviparus viviparus L. (1973)
    Springer
    Cell & tissue research 143 (1973), S. 329-341 
    Details
    ISSN: 1432-0878
    Keywords: Sites of ultrafiltration ; Gastropoda ; Podocytes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the Gastropoda pressure-ultrafiltration of the blood is assumed to be the first step in urine formation. The most probable site of ultrafiltration is the wall of the heart. Since in other animal groups ultrafilters are characterized by a special cell type, the podocyte, the hearts of two pulmonates (Lymnaea stagnalis, Biomphalaria glabrata) and of four prosobranchs (Viviparus viviparus, Bithynia tentaculata, Ampullaria gigas, Littorina littorea) were ultrastructurally investigated, in order to establish whether or not podocytes occur in these structures. It appeared that only in the wall of the auricle of Viviparus podocytes are present. They form a layer underneath the epicardium, the epithelium covering the auricle. It is assumed that in Viviparus ultrafiltration proceeds in the auricle. The possible route of the pro-urine is discussed. The location of the ultrafilters in the other species studied remains still unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307419
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    Studies on the surface coat of Paramecium aurelia (1973)
    Springer
    Cell & tissue research 143 (1973), S. 343-353 
    Details
    ISSN: 1432-0878
    Keywords: Surface coat (Paramecium aurelia) ; Ruthenium red staining ; Enzyme treatment ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The plasma membrane of Paramecium aurelia is covered with a ruthenium red stainable surface coat. Results obtained after digestion with pronase, trypsin and neuraminidase suggest the glycoprotein nature of this structure. Lipid extraction also affects the surface coat forming material. The results are consistent with the model proposed by Ginsburg and Kobata dealing with spatial configuration of the surface coat components.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307420
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    Ultrastructure de l'épithélium tégumentaire de Glossiphonia complanata (L.) (Hirudinée): cellules épithéliales et organes sensoriels de Bayer (1973)
    Springer
    Cell & tissue research 143 (1973), S. 355-365 
    Details
    ISSN: 1432-0878
    Keywords: Integument ; Hirudinea ; Sensory organs ; Nerves ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le tégument de Glossiphonia complanata comprend, outre les cellules épithéliales banales, de nombreux organes particuliers ou organes de Bayer, regroupés essentiellement sur la face dorsale de l'animal. Ils sont formés par une cellule apicale saillante enchassée dans une cellule musculaire en anneau. L'ensemble de ces formations est étudié du point de vue ultrastructural. Des cellules épithéliales partent des fibres nerveuses afférentes, sans doute vecteur des perceptions de stimuli mécaniques de pression au niveau du tégument; la réponse se faisant sans doute par la contraction de la cellule basale de chaque organe de Bayer, innervée par des fibres nerveuses efférentes, entrainant la saillie de la cellule apicale. Le hérissement de ces nombreuses papilles du tégument dorsal pourrait être un signal perçu par le partenaire sexuel, chez cette Hirudinée à fécondation hypodermique.
    Notes: Summary The integument of Glossiphonia complanata, built up by epithelial cells, contains numerous particular organs (Bayer organs), mainly on the dorsal side of the animal. They consist of a protuberant apical cell, which is surrounded by a ring-shaped muscle cell. All the integumentary formations are studied from an ultrastructural point of view. From the epithelial cells issue afferent nerve fibres, considered as vectors of the perception of mechanical stimuli of pressure at the level of the integument; the response no doubt operating by the contraction of the basal muscle cell of each Bayer organ, innervated by efferent nerve fibres, bringing forth the protrusion of the apical cell. The erection of these numerous papillae of the dorsal integument might be a signal perceived by the sexual partner, fecundation occurring in this group of leeches under the integument.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307421
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    Effects of testosterone, hydrocortisone and insulin on the fine structure of the epithelium of rat ventral prostate in organ culture (1973)
    Springer
    Cell & tissue research 143 (1973), S. 425-438 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Tissue culture ; Androgens ; Hormones ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The explants of the rat ventral prostate were cultured on a chemically defined medium and the effects of individual hormones viz. testosterone, hydrocortisone and insulin on the fine structure of the epithelial cells were investigated. The epithelial cells without hormones underwent regressive changes resembling those seen in vivo after castration. Large autophagic vacuoles, residual bodies and dilations of rough endoplasmic reticulum (ER) were noted on day 2 of the culture. Four days later the amount of rough ER was markedly decreased and no secretory vesicles were visible. The treatment of the explants with testosterone resulted in an apparent intensification of the morphological equivalents of secretion. Beside the well developed rough ER and Golgi complex, numerous large secretory vesicles with flocculent content were observed. These observations are compatible with the idea that testosterone promotes the formation and probably also the excretion of the secretion in the epithelial cells. Testosterone also prevented partially the appearance of the signs of cellular degeneration. The most characteristic feature of hydrocortisone-treated epithelial cells was the copious supply of rough ER. Of particular interest was the formation of endoplasmic reticulum whorls which could not be observed in control and testosterone-treated cells. Hydrocortisone was shown to promote the formation of juvenile secretory vesicles on the second day. Insulin delayed the onset of the degeneration of the epithelial cells. Marked degenerative changes were, however, noted on day 6. There was no specific ultrastructural characteristic that could be interpreted to be specific for insulin action in the epithelial cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307425
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    Unique sensory endings in rat muscle spindles (1973)
    Springer
    Cell & tissue research 136 (1973), S. 511-520 
    Details
    ISSN: 1432-0878
    Keywords: Muscle spindle ; Sensory ending ; Rat ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A fine structural study of sternothyroid muscle spindles of young adult rat demonstrates the rare occurrence of unique nerve endings. These endings are situated in the juxta-equatorial region of nuclear-bag fibers, adjacent to the annulo-spiral sensory endings. They consist of a bundle of terminal axons less than 0.3 μ in diameter and appear to be disposed nearly longitudinally to the axis of the intrafusal fibers. Whereas, the annulo-spiral endings consist of a single axon, coiling around the intrafusal fibers. Transverse sections of these muscle spindles reveal these unique endings scattered around the muscle fibers, fitting into depressions on their surface. The innermost axons directly face the muscle surface and are separated by a narrow gap less than 200 Å in width. No Schwann cell process appears to be associated with these endings. From their unique multi-axonal composition, these endings are termed “bundled endings”. Investigations of developing muscle spindles show the occasional presence of a similar multi-axonal composition of sensory endings in perinatal rats. It is suggested that “bundled endings” are sensory in nature, carrying an immature feature over to adult life.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307367
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    The fine structure of the branchial heart appendage of the cephalopod Octopus dofleini martini (1973)
    Springer
    Cell & tissue research 136 (1973), S. 545-568 
    Details
    ISSN: 1432-0878
    Keywords: Pericardial gland ; Octopus ; Excretory organ ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The branchial heart appendage of Octopus dofleini martini has been investigated electron microscopically. This organ is dominated by peripherally lobed blood sinuses. It contains free hemocyanin (often aligned in rows), amoebocytes, endothelial cells, and muscle cells which occur mainly in connection with neurons. The neurons are often exposed to the blood. The blood sinuses are enclosed by a basement membrane which contains collagen equivalents and fine fibrillar elements. The sinuses are covered by two different epithelia: 1) the epithelium in the caoity of the appendage consisting of irregularly shaped cells with processes, the so called (∼ 30 μ high) podocytes, and 2) the epithelium (∼ 40 μ in height) on the surface of the organ, which is composed of two parts: a) a “lacuna”-forming portion directly adjacent to the basement membrane, which is topped by b) a continuous tissue portion with occasional “lacuna”-canals. The intercellular spaces of the inner and outer epithelium are connected. The structures of these epithelial cells are discussed in relation to the formation of the pericardial fluid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307370
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    Synaptic structure in the visceral ganglion of the lamellibranch mollusc, Spisula solida (1973)
    Springer
    Cell & tissue research 138 (1973), S. 75-83 
    Details
    ISSN: 1432-0878
    Keywords: Invertebrate ganglia ; Spisula solida ; ynapses ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An examination using the electron microscope was carried out on the visceral ganglion of the marine bivalve mollusc Spisula solida. A range of fixation, block staining and section staining technique was used to study the structure of chemical synapses. Phosphotungstic acid employed as a block stain specifically stained pre- and post-synaptic structures associated with the membrane at synapses as well as one class of granular vesicle. The specialised contacts were however shown to be rare and in many parts completely absent. Many axons, containing several types of vesicle, were shown to be varicose and it is proposed that they may function in a similar way to the unspecialised varicose terminals of vertebrate autonomic neurons. The role of membrane specialisations in intercellular adhesion is discussed. This study concludes that many synapses may be morphologically unidentified using present criteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307079
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    Spermatogenesis revisited (1973)
    Springer
    Cell & tissue research 138 (1973), S. 155-169 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Mouse ; Multinucleate spermatids ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This ultrastructural study confirms and extends the light microscope findings of Bryan (1971) concerning the presence and developmental fate of multinucleate spermatids. Four main classes of cells: uninucleate-individual, uninucleate-conjoined, multinucleate-conjoined, and multinucleate-individual, were identified along with a few instances of more complex syncytial organizations. When the respective nuclei in a given multinucleate are far enough apart, each develops autonomously but in synchrony with its neighbors. When nuclei are intimately associated, the “normal” pattern of spermiogenesis may be altered, giving rise to highly bizarre spermatozoa. Commonly, a single Golgi complex serves a pair of nuclei and gives rise to a “T-shaped” acrosome which binds the nuclei together. During the ensuing nuclear elongation phase, such units are invested by a single manchette. Pairs of axonemes within a common plasma membrane have also been encountered. These ultrastructural findings indicate that multinucleate spermatids are true components (not artifacts) of the seminiferous epithelium of normal animals. The presence of such cells and the unusual developmental consequences which can arise as a result of the multinucleate state must be taken into account when evaluating the course of spermatogenesis in cases of mutation- or chemically-induced infertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306605
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    The epithelium of the middle ear in the guinea pig (1973)
    Springer
    Cell & tissue research 138 (1973), S. 283-294 
    Details
    ISSN: 1432-0878
    Keywords: Middle ear ; Guinea pig ; Epithelial cells ; Intercellular junctions ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An electron microscopic study of aldehyde and osmium fixed normal guinea pig middle ear epithelium was made. Numerous branching microvilli occur between the cilia of the ciliated cells. The granules of the secretory cells are always surrounded by a membrane, and they vary in their content of electron dense substance. Half desmosomes are frequent in basal cells. The squamous epithelial cells of the bulla contain few microvilli and pinocytoric invaginations. In the basal part of the squamous epithelium dilations of the intercellular clefts often occur. The luminal part of the intercellular clefts are closed by multiple tight junctions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306613
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    Der praecorneale Glykosaminoglykan-Film beim Haushuhn (1973)
    Springer
    Cell & tissue research 138 (1973), S. 295-298 
    Details
    ISSN: 1432-0878
    Keywords: Cornea epithelium ; Precorneal film ; Domestic fowl ; Ruthenium red ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das vordere Corneaepithel des Haushuhnes besteht aus 5 Schichten. Die oberflächlichste Lage hat einen Zottenbesatz. Die 0,2 μ langen Mikrovilli haben eine Dicke von 0,04–0,05 μ und einen Abstand von höchstens 0,1 μ voneinander. Auf der freien Oberfläche liegt ein mit Rutheniumrot anfärbbarer Film von Glykosaminoglykanen von 0,26–0,3 μ Dicke. Die Mikrovilli ragen in diesen Film hinein. Dieser Film ist für den spiegelnden Glanz der Cornea verantwortlich. Die Tränensekretion kann sehr gering sein, weil ein Teil der Flüssigkeit an die sauren Mukopolysaccharide gebunden ist. Die Moleküle dieses Films werden wahrscheinlich in der oberen Zellage synthetisiert.
    Notes: Summary The anterior cornea epithelium of the house-hen consists of 5 layers. The outermost layer has a villus border. The 0.2 μ long microvilli are 0.04–0.05 μ thick and are not more than 0.1 μ apart. A film of ruthenium red stainable glycosamine Glycans of 0.26–0.3 μ thickness lies on the free surface. The microvilli protrude into this film. This film is responsible for the reflecting shine of the cornea. The tear secretion can be kept at a low level as a part of the fluid is bound to the acid mucopolysaccharides. The molecules for this film are probably synthesized in the outermost cell layer.
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    URL: http://dx.doi.org/10.1007/BF00306614
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    Thyroidectomy cells and their response to thyrotrophin releasing hormone (TRH) in the rat (1973)
    Springer
    Cell & tissue research 138 (1973), S. 327-332 
    Details
    ISSN: 1432-0878
    Keywords: Thyroidectomy cell ; Pituitary gland ; Electron microscopy ; TRH and granule extrusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thyroidectomy cells of the rat pituitary gland were studied by the peroxidase-antibody labeling procedure and by electron microscopy. Secretory granules accumulated in these cells in response to a short-term treatment with thyroxine, and the cells were then reactive to the peroxidase-antibody labeling procedure. An intravenous injection of synthetic thyrotrophin releasing hormone (TRH) to thyroxine-treated, thyroidectomized rats provoked an acute and active extrusion of secretory granules from the thyroidectomy cells. The secretory granules in these cells were mostly “haloed” after primary fixation in osmium tetroxide. It is concluded that TRH causes thyroidectomy cells to release their secretory granules, and presumably TSH, by the usual process of exocytosis or granule extrusion.
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    URL: http://dx.doi.org/10.1007/BF00307096
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    Adrenergic and cholinergic nerves of bovine, guinea pig and hamster salivary glands (1973)
    Springer
    Cell & tissue research 138 (1973), S. 407-420 
    Details
    ISSN: 1432-0878
    Keywords: Salivary glands ; Autonomic innervation ; Acetylcholine-esterase ; Fluorescence microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of formaldehyde-induced fluorescence and acetylcholine-esterase (AChE) activity was histochemically investigated in certain salivary glands of the cow (submandibular gland), guinea pig and hamster (submandibular and sublingual glands). Adrenergic nerves occur around the secretory acini of the bovine, guinea pig and hamster submandibular glands, as well as around those of the hamster sublingual gland. The mucous secretory acini of the guinea pig sublingual gland, however, seem to be devoid of adrenergic nerve supply. Except in the sublingual gland of the hamster, no adrenergic nerves occur in relation to duct cells. The pattern of AChE activity is similar to that of adrenergic nerves. Thus, AChE-positive nerves form a network around secretory acini of all the five glands examined. Furthermore, AChE activity was also observed in nerve fibres in close proximity to striated duct cells. Both adrenergic and AChE-containing fibres were observed around blood vessels of different sizes. Ganglionic cells are occasionally to be seen; they all display AChE-activity. No adrenergic ganglionic cells were observed in any of the glands examined. All glands were also studied in the electron microscope. Interest was focussed on the fine structure of the autonomic nerves with special reference to their contents and type of storage vesicles. The content of noradrenaline was chemically determined in each type of salivary gland studied.
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    URL: http://dx.doi.org/10.1007/BF00307102
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    Elektronenmikroskopische Untersuchungen zur funktionellen Morphologie des Tubero-hypophysären Systems der Ratte (1973)
    Springer
    Cell & tissue research 139 (1973), S. 101-148 
    Details
    ISSN: 1432-0878
    Keywords: Infundibulum ; Rat ; Neuro-glial synapses ; Neuro-Vascular contacts ; Corticotropin-Releasing Factor ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Die Gefäßfortsätze von ependymalen und subependymalen Zellen bilden in der subependymalen Zone und in der Zona interna des Ratteninfundibulum mit Nervenfasern kompakte neuro-gliöse Faserbündel, die sich in der Zona externa aufzweigen. 2. In allen Zonen des Infundibulum kommen zwischen den Nervenfasern und den Gefäßfortsätzen zahlreiche neuro-gliöse Synapsen vor. In den Gefäßfortsätzen fällt die hohe Zahl an Mikrotubuli sowie die zahlreichen, vielgestaltigen Einschlüsse auf. 3. In der subependymalen Zone sind die Interzellularspalten an bestimmten Stellen außerordentlich weit. Sie haben eine kanalartige Beschaffenheit und enthalten feine Bündel von Nervenfasern. 4. Von den lateralen Anteilen des Infundibulum her erreichen Dendriten von Ganglienzellen des Nucleus infundibularis die Mitte des Infundibulum. In dieser Region sind axodendritische Synapsen anzutreffen. 5. Morphometrische Analysen der Nervenfaserendigungen der Zona externa von Normaltieren zeigen, daß die prozentuale Verteilung der nach Granulagröße differenzierten Nervenfaserklassen für Mitte und Seite der Zona externa etwa gleich ist. Zwischen der Größe der Elementargranula und der Anschnittfläche der zugehörigen Nervenfasern besteht eine direkte Beziehung. 6. Die Nervenfaserendigungen erreichen die Basalmembran des perikapillären Raumes fast ausschließlich im Bereich von gefäßwärts gerichteten Vorwölbungen der Zona externa. Das Ausmaß, in dem Nervenfasern im Vergleich zu den Gefäßfortsätzen von Ependymund Gliazellen den perivaskulären Raum erreichen, ist medial weitaus größer als lateral. 7. Bei bilateral adrenalektomierten Ratten nimmt in bestimmten, vorwiegend lateral gelegenen Nervenfasern die Zahl und Größe der Elementargranula in Abhängigkeit von der Überlebensdauer zu. Dies dürfte auf eine verstärkte Synthese und Speicherung von Corticotropin-Releasing Factor in diesen Nervenfasern zurückzuführen sein. Gegenüber dem Normalbefund ist die neurohämale Kontaktfläche erheblich vergrößert. Der perivaskuläre Raum enthält zerfallene Nervenfaserteile, die durch Bindegewebszellen phagocytiert werden. Diese Veränderungen dürften durch eine unter Versuchsbedingungen verstärkte Wachstumstendenz der Nervenfasern in Richtung auf die Blutgefäße und durch eine Abschnürung der Nerven-faserendigungen ausgelöst werden.
    Notes: Summary 1. In the subependymal and internal zones of the rat median eminence nerve fibres and vascular processes of ependymal and subependymal cells form neuro-glial bundles. They branch in the external zone. 2. In all these three zones of the infundibulum numerous neuro-glial synapses are found between nerve fibres and vascular processes of glial cells. The vascular processes contain a high number of microtubules as well as polymorphous granular inclusions. 3. In certain regions of the subependymal layer the intercellular spaces are enlarged. They form channel-like spaces containing small bundles of delicate nerve fibres. 4. Nerve cells of the infundibular nucleus located in the lateral parts of the infundibulum send dendrites to the medial parts of the infundibulum. In this area axo-dendritic synapses are found. 5. For morphometric analysis, the nerve fibres of the external zone were classified according to the diameter of their granules. It is shown that in the different regions of the external zone the distribution of the various types of nerve fibre is similar. Moreover it can be seen that a direct correlation exists between the size of the sectional plane of a given nerve fibre and the size of the granules it comprises. 6. Nerve fibre endings abutting on the basement membrane of the pericapillary space are mostly found in bulb-like protrusions of the external zone. The extent to which nerve fibres reach the perivascular space—as compared with the vascular processes of ependymal and glial cells—is higher in the medial than in the lateral parts of the infundibulum. 7. In bilaterally adrenalectomized rats the number and diameter of elementary granules increases in nerve fibres located laterally. This increase is directly related to the survival time and may be due to an enhanced synthesis and storage of Corticotropin-Releasing Factor in these nerve fibres. Compared with the findings in untreated animals the neurohemal contact area is significantly enlarged. The perivascular space contains degenerating nerve fibres which are undergoing phagocytosis by connective tissue cells. It is assumed that these alterations are due to the increased growth of nerve fibres towards the vessels of the “Mantelplexus”, and that, following adrenalectomy, this excessive growth leads to a pinching off of nerve fibres.
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    URL: http://dx.doi.org/10.1007/BF00307465
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    Elektronenmikroskopische und histochemische Untersuchungen zur Funktion der Branchialdrüse (Parabranchialdrüse) der Cephalopoda (1973)
    Springer
    Cell & tissue research 139 (1973), S. 253-269 
    Details
    ISSN: 1432-0878
    Keywords: Branchial gland ; Cephalopoda ; Fine structure ; Hemocyanin ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Licht- und elektronenmikroskopische Untersuchungen an der Branchialdrüse verschiedener Cephalopoden (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) zeigen, daß das Drüsengewebe des stark vaskularisierten Organs aus einem sekretorisch aktiven Zelltypus mit ausgeprägtem endoplasmatischen Retikulum besteht. In den Zisternen des endoplasmatischen Retikulums lassen sich granuläre und ringförmige Strukturen (Durchmesser: 45–65 Å und 170 Å) nachweisen, die in Größe und Gestalt mit den Hämocyanin-Einheiten in den Gefäßen und Lakunen übereinstimmen und durch randlichen Zerfall der Zellen in den Blutraum auszuwandern scheinen. Die histochemischen Untersuchungen ergeben eine positive Reaktion für Monoaminoxydase, aber vergleichsweise geringe Phosphatase- und Dehydrogenase-Aktivitäten. Diese Befunde sowie der histochemisch nachgewiesene hohe Kupfergehalt des Drüsengewebes sprechen für eine hämopoetische, d.h. hämocyaninbildende Funktion der Kiemendrüse.
    Notes: Summary Light and electron microscopic investigations on the branchial gland of different Cephalopoda (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) show that the gland tissue, strongly vascularized, consists of an actively secreting cell type with a remarkable endoplasmatic reticulum. In the cisternae of the endoplasmatic reticulum granular and ring-shaped structures can be identified (diameter: 45–65 Å and 170 Å) which correspond in size and shape with the hemocyanin units in the vessels and lacunae and which seem to emigrate into the blood space caused by the peripheral decay of the cells. Histochemical investigations show a positive reaction for monoaminoxidase, but comparatively low phosphatase and dehydrogenase activities. These results, and also the high content of histochemically identified copper in the gland tissue, seem to indicate a hemopoetic, i.e. hemocyanin-forming function for the branchial gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306525
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  • 97
    Electronic Resource
    Electronic Resource
    Electron microscopic observations on the mucous secretory activity of the human gallbladder epithelium (1973)
    Springer
    Cell & tissue research 139 (1973), S. 463-471 
    Details
    ISSN: 1432-0878
    Keywords: Gallbladder (Human) ; Mucus secretion ; Reverse pinocytosis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mucous secretory activity of the human gallbladder epithelium was investigated by light and electron microscopy and with histochemical techniques. There are two types of granules in the supranuclear region of the epithelial cells. The one low in density contains a fine filamentous material and gives a strongly positive silver methenamine reaction. The other is dense and only faintly positive. The granules of the former are considered to be mucous secretory granules and the granules of the latter may be lysosomes. PAS positive granules correspond presumably to both types of granules mentioned above. The mucous secretory granules are considered to be synthesized by the Golgi apparatus and the granular endoplasmic reticulum as has been confirmed in other mucous secretory cells. Their content is released from the cell by reverse pinocytosis. Typical goblet cells occur frequently in the fetal epithelium, but cannot be observed in the adult specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02028388
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  • 98
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    Electronic Resource
    An analysis of some aspects of synaptosomal ultrastructure by the use of serial sections (1973)
    Springer
    Cell & tissue research 140 (1973), S. 481-496 
    Details
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Dense projections ; Complex vesicles ; Serial sections ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptosomes and synaptic junctions have been examined employing serial sections, with emphasis placed on four areas of investigation. 1. Starting from unequivocal synaptosomal profiles and tracing them through consecutive sections to the periphery of the synaptosomes, it is clear that vesicles are the one constant feature of the presynaptic terminal. In no instance was it possible to identify an empty membranous profile as synaptosomal. 2. Following a similar procedure it was found that the criteria required to predict the existence of a junctional region within a synaptosomal profile are: the accumulation of synaptic vesicles at one locus within its presynaptic component, and the presence of a postsynaptic profile characterized by two or more junctional features. 3. Serial sections of non-osmicated, PTA stained synaptic junctions confirm the regularity and orderliness of dense projection distribution along the length of the junction. 4. Complex vesicles can usually be followed in two and sometimes three adjacent sections, appearing either as intact vesicles or empty shells. Further observations confirmed that the latter profiles may be sections through the periphery of intact vesicles or through isolated shell fragments. They are more common in the latter form in unbuffered material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306675
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  • 99
    Electronic Resource
    Electronic Resource
    Ultrastructure of the sexual segments of the kidney in male and female lizards, Cnemidophorus l. lemniscatus (L.) (1973)
    Springer
    Cell & tissue research 144 (1973), S. 325-337 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Sexual accessory organs ; Reptiles ; Secretion ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Kidneys of adult male and female lizards were studied by electron microscopy, in order to understand the ultrastructure of the collecting duct and a differentiated part thereof, the sexual segment, which is an important accessory sexual organ. First portion of sexual segment in males: The cells are filled with large secretory granules of a wide range of opacities. The granular endoplasmic reticulum is abundant; basal formations of superimposed flat cisternae are frequent. Distended vesicles and microvesicles prevail in the supranuclear, well developed Golgi apparatus. Evidences indicate that secretion of these cells is holocrine. Second portion of sexual segment in males: All of the secretory granules are apical in location and relatively electron-opaque; they show a denser core. This core is formed by a substance which, after lying in contact with ribosomes, enters the secretory vesicles of the highly developed Golgi apparatus. A lighter substance is then condensed around it. The secretion of the granules is merocrine. The granular endoplasmic reticulum is very abundant in these cells, but basal ergastoplasmic formations are lacking. Sexual segment in females: The cells show features similar to those of the male first portion, but they are smaller. Undifferentiated collecting duct: Most of the cells are mucigenic. They have small ovoid, apical secretory granules. The density of the granules varies from cell to cell; when they are electron-lucent, they exhibit laminar or dotted opaque figures. Moderately developed Golgi apparatus and granular endoplasmic reticulum, as well as elongated mitochondria, occur in mucigenic cells. Intercalated among the latter are non-secretory cells. They have very abundant mitochondria, numerous microvilli, many pinocytic and smooth-membrane vesicles, whereas the organelles participating in synthetic processes are poorly developed; their function is most likely related to active solute transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307580
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  • 100
    Electronic Resource
    Electronic Resource
    Ultrastructure of subcellular fractions of bull spermatozoa after hyamine treatment (1973)
    Springer
    Cell & tissue research 144 (1973), S. 395-408 
    Details
    ISSN: 1432-0878
    Keywords: Spermatozoa ; Cattle ; Influence of cationic surface active agent ; Acrosome reaction ; Centrifugation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ejaculated bull spermatozoa (SZ) were washed and incubated with a cationic surface active agent, Hyamine 2389, and centrifuged using 2-step discontinuous sucrose density gradient. The washed SZ, Hyamine-treated SZ and subcellular spermatozoal fractions obtained after centrifugation were prepared for electron microscopy. The washing did not cause any major structural changes in SZ. The Hyamine treatment of SZ disrupted the outer acrosome membranes. The anterior part of acrosome (the acrosomal cap) was detached retaining its integrity, or forming vesicles by fusing with the cell membrane as in “true” acrosome reaction. Because of this structural similarity in vesicle formation, Hyamine is assumed to be a suitable experimental initiator for acrosome reaction. The loosened acrosomal membranes were harvested almost totally by the centrifugation. The acrosomes were seen as loosened U-shaped unbroken acrosomal caps or as vesicles with fuzzy acrosomal material. The lightest particles were vesicles consisting of smooth membranes, formed evidently of sperm cell membrane. A negligible amount of fibrous sheaths were also among acrosomal membranes but no other sperm parts were encountered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307584
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