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  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of histamine release from rat peritoneal mast cells by a factor from human serum — identification as transferrin (1986)
    Springer
    Inflammation research 19 (1986), S. 10-17 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Recently, we described the presence of a blocking factor (BF) in rat serum which inhibited the histamine release from rat mast cellsin vivo andin vitro. The blocking activity was demonstrated in human serum as well. Purification of the human-BF was carried out in a similar way as previously described for the rodent molecule. Both protein fractions produced a marked suppression of histamine release from rat mast cells and human basophils in a dose-dependent fashion. Qualitative analysis of the purified preparations demonstrated a major component with a molecular weight of 70 000 daltons. In human serum the blocking factor was identified as transferrin by serological and biochemical methods. It is suggested that this molecule may play an important role in regulating histamine release during allergic and inflammatory reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01977250
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells (1986)
    Springer
    Inflammation research 17 (1986), S. 418-426 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The close relation between rat mast cells and rat basophilic leukemia (RBL) cells with regard to the presence of receptors for IgE and Fcγ led us to generate monoclonal antobodies directed against cell surface antigens. Hybridomas were obtained by the fusion of NS1 mouse myeloma cells with murine spleen and lymph node cells. The culture supernatants were assayed by two ELISA techniques: a) for the production of mouse immunoglobulin in general and b) for antibodies directed against surface antigens of RBL cells. For this purpose RBL cells were attached to polyvinyl chloride microtitre plates. Eight hybrids produced antibodies directed against surface antigens on RBL cells. Hybrids were cloned and characterized with regard to their isotype and light chains. All eight clones secreted IgM with K light chains. Immunofluorescence studies performed with RBL cells revealed that all eight antibodies were able to show a specific fluorescence. Furthermore, four of these eight antibodies also showed a specific fluorescence with purified rat mast cells. These four antibodies were analyzed as to their ability of interacting with the IgE-receptor on RBL cells and purified rat mast cells. They reduced the binding rate of radiolabelled rat IgE to RBL and rat mast cells. A mutual inhibition of the passive cutaneous anaphylaxis (PCA) reaction in the rat by either mixing mouse reaginic serum directed against 2,4-dinitrophenol bovine serum albumin (DNP-BSA) or by mixing monoclonal mouse anti-DNP IgE with the monoclonal mouse anti-cell surface (rat basophilic leukemia, rat mast cell) IgM was determined. The histamine release of rat mast cells obtained from Nippo-strongylus brasiliensis infected rats was markedly reduced in the presence of two monoclonal anti cell surface antibodies. Our data suggest that monoclonal antibodies with specificity for the IgE receptor (inhibition of IgE-binding) can be distinguished from those which bind closely to the IgE-receptor but also modulate the histamine release from presensitized mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01965508
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  • 3
    Electronic Resource
    Electronic Resource
    Determinanten von Digoxin-und Digitoxinplasmakonzentrationen bei älteren Patienten (1987)
    Springer
    Journal of molecular medicine 65 (1987), S. 1073-1080 
    Details
    ISSN: 1432-1440
    Keywords: Digoxin derivatives ; Digitoxin ; Variables determining plasma concentration ; Multiple linear regression analyses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 1063 patients (≥60 years, 531 men, 532 women) the plasma concentration during digitalis maintenance therapy (metildigoxin, n=356, beta-acetyldigoxin, n=359, and digitoxin, n=348) was determined and related to sex, age, body weight, serum potassium, renal function and the prescribed daily maintenance dose. Classification of treatment groups according to renal function (Crea ≤1.3 mg/dl//〉 1.3 mg/dl) did not show any difference of the mean maintenance doses. In multiple linear regression analyses only a weak relationship between plasma digitalis concentration and the studied variables was found, which could be equally attributed to dose, creatinine and serum potassium in the digoxin derivative groups, whereas for digitoxin only body weight had a significant effect on the plasma concentration. During a maintenance dose of 0.07 or 0.1 mg/die which was given to 87% of patients in the digitoxin group, 70% were found to have plasma levels within the therapeutic range.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01736113
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  • 4
    Electronic Resource
    Electronic Resource
    Treatment of hypertension (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 1229-1236 
    Details
    ISSN: 1432-1440
    Keywords: High blood pressure ; Epidemiologic study ; Treatment ; Control ; Drug utilization ; Electrocardiogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the Munich Blood Pressure Study (MBS), a cross-sectional study (MBS I) with follow-up (MBS II) of a random sample of 3,198 citizens aged 30–69 (response rate, 69.3%), treatment and control of hypertension were examined. Of the actual hypertensives 59% (221) in MBS II (373) were on drug treatment and about two-thirds (150) of those being treated had controlled blood pressure (BP). Women's BP was better controlled than men's. Of the 221 treated hypertensives 85% (188) received types of drugs or drug combinations which were in accordance with the recommendations of the German Hypertension League. However, this did not apply to the prescribed dosages of the various drugs. Of the treated hypertensives 52% were either on a diuretic, on a beta-blocker, or on a combination of both drugs. Rauwolfia alkaloids combined with a diuretic were given to 27%. In 79% of the participants who had received a beta-blocker either alone or in combination with other antihypertensive agents and who still had high BP values, the prescribed beta-blocker dosage was below the recommended daily dose. Fatigue was the most frequently reported symptom, possibly attributable to antihypertensive drug treatment. Electrocardiographic signs of left ventricular hypertrophy were found less frequently in controlled hypertensives, in comparison to treated but uncontrolled hypertensives or untreated hypertensives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01734464
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  • 5
    Electronic Resource
    Electronic Resource
    Die Bedeutung der Leukotriene bei chronischen Atemwegserkrankungen im Kindesalter (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 257-264 
    Details
    ISSN: 1432-1440
    Keywords: Asthma ; Bronchitis ; Leukotrienes ; HPLC ; RIA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The role of lipoxygenase products was studied in children suffering from chronic diseases of the lung. Leukotrienes C4, D4, E4 and B4 were measured by high performance liquid chromatography (HPLC) and a specific radioimmunoassay (RIA) for C4. Elevated levels (up to 40 ng/ml), especially for leukotriene E4, were found in plasma of asthmatic and bronchitic patients (leukotriene C4 concentrations varied between 0.05 and 40 ng/ml, mean 4.9±7.8 ng/ml). In healthy donors the concentrations were below the detection limits of HPLC, leukotriene C4 ranging between 5±4 ng/ml (RIA data). The conversion of leukotriene C4 to D4 and E4 was observed by incubating the samples with synthetic leukotriene C4. The half-life of leukotriene C4 in plasma varied greatly, ranging from 〈12 min to 72 min (mean 39±16 min). Bronchial lavages yielded leukotriene C4 concentrations of 0.2 to 7 ng. Leukotriene E4 was detected in 10 of 41 cases. Conversion of leukotriene C4 did not occur in 50% of all cases, but was regularly observed in putrid lavages. These data suggest that leukotrienes play an important role in allergic and infectious lung diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01711931
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  • 6
    Electronic Resource
    Electronic Resource
    Orinithine as a constituent of the peptidoglycan of Chloroflexus aurantiacus, diaminopimelic acid in that of Chlorobium vibrioforme f. thiosulfatophilum (1987)
    Springer
    Archives of microbiology 148 (1987), S. 72-76 
    Details
    ISSN: 1432-072X
    Keywords: Chlorobium ; Chloroflexus ; Cell wall ; Diaminopimelic acid ; Muramic acid-6-phosphate ; Ornithine ; Peptidoglycan-polysaccharide complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract L-Ornithine is the only diamino acid of the peptidoglycan of the gliding phototrophic Chloroflexus aurantiacus. The other constituents are L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (in part as muramic acid-6-phosphate), all in approximate equimolar ratios to L-ornithine, aside from small amounts of glycine and histidine. Furthermore unlike typical Gram-negative bacteria, protein is not bound to this peptidoglycan. Instead, the rigid layer (sodium dodecyl sulfate insoluble cell wall fraction) contained large amounts of a complex polysaccharide consisting of sugar O-methyl ethers, hexoses and pentoses. Its binding site is presumably muramic acid-6-phosphate of the peptidoglycan. In contrast, in Chlorobium vibrioforme f. thiosulfatophilium, meso-diaminopimelic acid was found as the only diamino acid of this peptidoglycan. As with other Gramnegative bacteria, L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (no muramic acid-6-phosphate) were observed in approximate equimolar ratios to meso-diaminopimelic acid, except a lower D-alanine content. The rigid layer of Chlorobium vibrioforme f. thiosulfatophilum contained protein, and there were no indications for a complex polysaccharide comparable to that of Chloroflexus aurantiacus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429651
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  • 7
    Electronic Resource
    Electronic Resource
    Nontoxic and toxic oligopeptides with D-amino acids and unusual residues in Microcystis aeruginosa PCC 7806 (1989)
    Springer
    Archives of microbiology 151 (1989), S. 411-415 
    Details
    ISSN: 1432-072X
    Keywords: Microcystis aeruginosa ; Cyanobacterium ; Toxin ; Oligopeptides ; D-Amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Toxic and nontoxic peptides were isolated from the cyanobacterium Microcystis aeruginosa PCC 7806 by a procedure including extraction of cells with water-saturated 1-butanol, chromatography of the extract on silica gel plates and high performance liquid chromatography (HPLC) on Partisil-5. The toxin was shown to be only a minor constituent, being negatively charged and thus separable by electrophoresis, within the HPLC-purified fraction. It contained erythro-β-methyl-D-Asp, D-Glu, D-Ala, L-Leu, and L-Arg known to be part of the Microcystis peptide-toxin with Mr 994. The major part of the HPLC-purified fraction was assigned, however, to a nontoxic peptide with a Mr of 956. Partial hydrolysis studies of the nontoxic peptide(s) revealed amino acid sequences composed of D-Glu, N-methyl-Phe, and 3,4-dehydro-Pro, aside from the common L-amino acids. Cyclic linkage in the nontoxic peptide(s) appears likely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00416599
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  • 8
    Electronic Resource
    Electronic Resource
    Psychotropic drug utilization patterns in a metropolitan population (1987)
    Springer
    European journal of clinical pharmacology 32 (1987), S. 43-51 
    Details
    ISSN: 1432-1041
    Keywords: benzodiazepines ; psychotropic drug intake ; long-term use ; epidemiological study ; multiple logistic regression analysis ; drug interaction ; sex differences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Psychotropic drug intake by a random sample of citizens of the city of Munich aged 30–69 years has been assessed. A 1-week prevalence of 9.3% for all psychotropic drug users was found, benzodiazepines accounting for approximately two-thirds (6.6%) of the users. Two-thirds of drug users were women. Drug use in both sexes increased with age. The doses of benzodiazepines prescribed in most cases were less than 10 mg diazepam equivalent per day. Intake of benzodiazepines in combination with analgesics or alcohol (⩾40 g/day) did not appear to represent a major problem. Multiple logistic regression analysis showed that the number of chronic diseases was the strongest predictor of benzodiazepine intake in men, whereas stress and age determined intake in women. Long-term use seemed to be relatively rare at 11% of all benzodiazepine users, so it was not considered to be a severe public health problem.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00609956
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  • 9
    Electronic Resource
    Electronic Resource
    Contact allergy due to colophony (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Contact dermatitis 20 (1989), S. 0 
    Details
    ISSN: 1600-0536
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 7 resin acids, 3 symbolically prepared derivatives and the neutral fraction of Chinese colophony were studied by experimental sensitization using a modified FCA method. 4 resin acids, laevopimaric. abietic. podocarpic and tetrahydroabietic. proved to be weak sensitizers Neoabietic, dehydroabietic, isopimarie acid and larixol remained negative. However, the derivative, methyl abietate and calcium abietate. were shown to be moderate sensitizers. and the maleic-modified adduct to be a moderate to strong sensitizer. As laevopimaric acid plays only a minor rote in colophony, abietic acid must he considered a major, even if weak, allergen in non-modified colophony. The neutral fraction of colophony is also partially involved in colophony allergy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0536.1989.tb03094.x
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  • 10
    Electronic Resource
    Electronic Resource
    Allergic and irritant contact dermatitis from falcarinol and didehydrofalcarinol in common ivy (Hedera helix L.) (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Contact dermatitis 17 (1987), S. 0 
    Details
    ISSN: 1600-0536
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Experimental and chemical investigations revealed that common ivy (Hedera helix susp. Helix) contains 3 compounds which are powerful irritants and moderate sensitizers. Only 2 of these constituents, falcarinol and didehydrofalcarinol, are present in the plant during the whole year. Besides Panax ginseng and Schefflera arboricala, this is the third species of the Araliaceae in which these polyacetylenic sensitizers have been found. Falcarinol and didehydrofalcarinol also occur in Hedera helix subsp. canariensis. 4 patients have been patch tested. Even in low concentrations (0.03%). the main allergen falcarinol elicited strong reactions in all of them. One of the authors became sensitized during the investigations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0536.1987.tb02634.x
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