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  • 1
    Digitale Medien
    Digitale Medien
    Segregation of linked probes to myotonic dystrophy in a family demonstrating that 152 and APOC2 are on the same side of DM on 19q (1988)
    Springer
    Human genetics 〈Berlin〉 80 (1988), S. 379-381 
    Details
    ISSN: 1432-1203
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The two markers most closely linked to the myotonic dystrophy (DM) locus on chromosome 19 are the gene that codes for apolipoprotein CII (APOC2) and the anonymous probe D19S19 (LDR152). Both of these markers show tight linkage to DM, with maximum lod scores of〉20 at recombination fractions of less than 0.05. We have identified, in a family in which DM segregates, an affected individual where a meiotic recombination event has occurred in which both of these linked markers have crossed over with the gene defect. This demonstrates that APOC2 and D19S19 are probably on the same side of DM.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00273655
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  • 2
    Digitale Medien
    Digitale Medien
    Murine invariant chain gene: Chromosomal assignment and segregation in recombinant inbred strains (1985)
    Springer
    Immunogenetics 22 (1985), S. 193-199 
    Details
    ISSN: 1432-1211
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00563518
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  • 3
    Digitale Medien
    Digitale Medien
    A simple method for elution of IgA deposits from the skin of patients with dermatitis herpetiformis (1989)
    Springer
    Archives of dermatological research 281 (1989), S. 406-410 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Dermatitis herpetiformis ; Immunoglobulins ; Complement components ; Elution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary To better understand the role of autoimmunity in the pathogenesis of dermatitis herpetiformis, linear IgA bullous dermatosis or other skin disorders, the antigenic specificity of the immune reactants bound in vivo in the skin must be identified. In order to do so, one must first be able to elute these immune reactants from the skin. We describe here a simple method of eluting not only specifically bound IgG, but also IgA and other immunoglobulins and complement components from skin biopsy material. The method involves cutaneous washing of the entrapped serum proteins in PBS pH 7 and pH 5 buffers followed by specific immunoglobulin elutions at pH 3 and 2. The IgA deposits which could not be removed by this treatment were eluted by a combination of low pH (0.5 M citrate pH 2) and a chaotropic agent (2 M NaCl). The relative concentration of IgA in eluates when quantitated by fluoroimmunoassay were three-to five-fold higher in dermatitis herpetiformis skin biopsy specimens, than in eluates of bullous pemphigoid or normal skin biopsy specimens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00455326
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  • 4
    Digitale Medien
    Digitale Medien
    Non-tuberculous mycobacterial lymphadenitis in children (1989)
    Springer
    European journal of pediatrics 148 (1989), S. 751-754 
    Details
    ISSN: 1432-1076
    Schlagwort(e): Lymphadenopathy ; Atypical mycobacteria ; Non-tuberculous mycobacterial lymphadenitis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Eighty-six children (44 males, 42 females) were identified as having non-tuberculous mycobacterial lymphadenitis. The diagnostic criteria were either culture of the organism from the affected lymph node (n=68), or, when culture was negative, a positive skin test with non-tuberculous mycobacterial antigens and negative skin test responses to tuberculin purified protein derivative (PPD) in association with typical histological features (n=18). All children had histopathological findings of granulomatous inflammation with caseation and/or acid-fast bacilli. Eighty-two percent of the children were under 5 years of age at presentation and 30% were less than 2 years old. Most (79%) were city dwellers. Lymph node enlargement had been present for less than 6 months in almost all children (97.5%) and was almost exclusively in the face and neck region (97%). Disease was confined to the involved lymph nodes in 56% but had extended beyond the confines of the infected node to form a collar stud abscess in 38% and 6% presented with a skin sinus. Extranodal extension did not show any statistically significant association with the duration of lymphadenopathy. The duration of lymphadenopathy had been greater in those children in whom an organism was not isolated on culture resected tissue χ2, P〈0.01). All children were treated surgically, and recurrence occurred in five patients. This study describes the clinical and demographic characteristics of non-tuberculous mycobacterial lymphadenopathy in children in a population in which tuberculous adenitis is rare. Recognition of these features may allow earlier diagnosis and appropriate surgical therapy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00443102
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  • 5
    Digitale Medien
    Digitale Medien
    Efficient and selective population of high vibrational levels by stimulated near resonance Raman scattering (1987)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 87 (1987), S. 5064-5076 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: We report the successful application of an optically pumped molecular beam laser for efficient and selective population transfer from v″=0 to high lying vibrational levels of Na2 in the electronic ground state. More than 50% of the molecules in a thermally populated rotational level of v″=0 can be transferred to a state v″(very-much-greater-than)1 with typically ≥75% of the molecules excited out of the initial state found in the predetermined end vibrational level and approximately ≤25% distributed over other levels according to the Franck–Condon factors. The applicability of this technique to the study of collision processes involving vibrationally highly excited levels is discussed. This work also contributes in general to a better understanding of optically pumped dimer lasers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.453674
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  • 6
    Digitale Medien
    Digitale Medien
    Mechanism of CO bond scission at alkali promoter sites—IRAS study of the system CO/K/Ni(111) (1987)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 86 (1987), S. 6523-6530 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: IRAS (infrared reflection absorption spectroscopy) has been employed to study the isotopic mixing reaction: 13C16O(a)+12C18O(a)→13C18O(a)+12C16O(a) on Ni(111) with preadsorbed K adatoms. Under high temperature conditions (T〉450 K) where the isotopically mixed CO is being desorbed, it has been shown that the isotopic exchange reaction cannot be detected by IRAS on the surface among CO molecules strongly interacting with K adatoms. This result implies that dissociation of CO occurs at K-promoted Ni sites followed by surface diffusion of C(a) and O(a) away from the K promoter site. Statistical recombination of C(a)+O(a) occurs with concomitant CO desorption on Ni(111) sites some distance from K centers. The empty K-promoted sites may be refilled by surface diffusion of CO from outside. Thus, alkali metal promotion of C–O bond scission may act via a "feeder-site'' mechanism connecting promoter atoms to external surface sites via surface diffusion of atomic C and O species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.452444
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  • 7
    Digitale Medien
    Digitale Medien
    Comment on: "The infrared spectrum of the ν3 band of TiF4'' (1989)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 90 (1989), S. 5209-5210 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.456542
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  • 8
    Digitale Medien
    Digitale Medien
    The determination of absolute structure. III. An ambiguity table for the non-centrosymmetric crystal classes (1986)
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 42 (1986), S. 57-57 
    Details
    ISSN: 1600-5724
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: The general term 'determination of absolute structure' is more precisely defined for each non-centrosymmetric crystal class.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0108767386099890
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  • 9
    Digitale Medien
    Digitale Medien
    The determination of absolute structure. V. The importance of absorption corrections (1987)
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 43 (1987), S. 79-80 
    Details
    ISSN: 1600-5724
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: Thirteen non-centrosymmetric structures with absorption coefficients ranging from 45 to 0.6 mm-1 were refined using both absorption-corrected and uncorrected data. The absorption was in no case severe, since \bar \mu r was less than 1 for each structure. In these circumstances, the absolute-structure parameter η could be determined equally clearly in both cases, although its e.s.d. increased somewhat for uncorrected data. More serious were the effects of uncorrected data on the precision of molecular dimensions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0108767387099847
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  • 10
    Digitale Medien
    Digitale Medien
    EXPRESSION OF HUMAN CD4 BY TWO HUMAN-MOUSE INTERLINEAGE HYBRIDS (1988)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 15 (1988), S. 0 
    Details
    ISSN: 1744-313X
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Two hybrid cell lines expressing human CD4 were prepared by fusing human B-lymphoid cells with the mouse T-lymphoma BW5147. Hybrid TF42 was derived from a human B-lymphoblastoid line and TF53.1 from a human B-ALL. Variants of these hybrids expressing or lacking CD4 were isolated by sorting cells stained with the monoclonal antibody (mAb) OKT4 on a fluorescence-activated cell sorter (FACS). Cytogenetic, isoenzyme and DNA analysis confirmed the presence of human chromosome 12 in the CD4+ hybrids, and revealed that CD4 expression by TF42 was associated with multiple copies of this chromosome. Of seventy mAb recognizing human T-cell antigens screened on the CD4+ and CD4− variants of the two hybrids, only mAb recognizing CD4 and Leu 8 reacted with the CD4+ cells. These hybrids should be useful in the preparation, screening and analysis of anti-CD4 monoclonal antibodies, and in studies of CD4 epitopes recognized by HIV.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1744-313X.1988.tb00422.x
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