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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] We found that PPM1D, encoding a serine/threonine protein phosphatase, lies within an epicenter of the region at 17q23 that is amplified in breast cancer. We show that overexpression of this gene confers two oncogenic phenotypes on cells in culture: attenuation of apoptosis induced by serum ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To date, more than 200 microRNAs have been described in humans; however, the precise functions of these regulatory, non-coding RNAs remains largely obscure. One cluster of microRNAs, the mir-17–92 polycistron, is located in a region of DNA that is amplified in human B-cell lymphomas. Here ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 397 (1982), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 318 (1985), S. 20-20 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-In a recent News and Views article on yeast cell biology (Nature 316, 678), I. HerSkowitz states that mutations in yeast that result in Gj arrest with reduced protein synthesis are relatively unlikely to reflect specific cell-cycle controls, in contrast with mutations such as cdc28, which ...
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1439-6327
    Keywords: Key words Diaphragm ; Oxidative stress ; Fatigue ; Lipid peroxidation ; Antioxidants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract These experiments tested the hypothesis that short-term endurance exercise training would rapidly improve (within 5 days) the diaphragm oxidative/antioxidant capacity and protect the diaphragm against contraction-induced oxidative stress. To test this postulate, male Sprague-Dawley rats (6 weeks old) ran on a motorized treadmill for 5 consecutive days (40–60 min · day−1) at approximately 65% maximal oxygen uptake. Costal diaphragm strips were excised from both sedentary control (CON, n=14) and trained (TR, n=13) animals 24 h after the last exercise session, for measurement of in vitro contraction properties and selected biochemical parameters of oxidative/antioxidant capacity. Training did not alter diaphragm force-frequency characteristics over a full range of submaximal and maximal stimulation frequencies (P 〉 0.05). In contrast, training improved diaphragm resistance to fatigue as contraction forces were better-maintained by the diaphragms of the TR animals during a submaximal 60-min fatigue protocol (P 〈 0.05). Following the fatigue protocol, diaphragm strips from the TR animals contained 30% lower concentrations of lipid hydroperoxides compared to CON (P 〈 0.05). Biochemical analysis revealed that exercise training increased diaphragm oxidative and antioxidant capacity (citrate synthase activity +18%, catalase activity +24%, total superoxide dismutase activity +20%, glutathione concentration +10%) (P 〈 0.05). These data indicate that short-term exercise training can rapidly elevate oxidative capacity as well as enzymatic and non-enzymatic antioxidant defenses in the diaphragm. Furthermore, this up-regulation in antioxidant defenses would be accompanied by a reduction in contraction-induced lipid peroxidation and an increased fatigue resistance.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1439-6327
    Keywords: Electrolytes ; Fluid balance ; Glucose ; Exercise metabolism ; Blood pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The purpose of these experiments was to examine the influence of various fluid replacement drinks on exercise-induced disturbances in homeostasis during heavy exercise. Nine trained cyclists performed constant load exercise on a cycle ergometer to fatigue on three occasions with 1-week separating experiments. The work rate was set initially at ∼ 85% of $$\dot V_{o_{2{\text{ }}max} } $$ (range 82–88%) with fatigue being defined as a 10% decline in power output below the initial value. During each experiment subjects consumed one of the following three beverages prior to and every 15 min during exercise: (1) non-electrolyte placebo (NEP; 31 mosmol · kg−1); (2) glucose polymer drink containing electrolytes (GP; 7% CHO, 231 mosmol · kg−1), and (3) electrolyte placebo drink without carbohydrate (EP; 48 mosmol · kg−1). Both the GP and EP beverage contained sodium citrate/citric acid (C) as a flavoring agent while C was not contained in the NEP drink. Although seven of nine subjects worked longer during the GP and EP treatment when compared with the NEP trial, the difference was not significant (P〉0.05). No differences (P〉0.05) existed between the GP and EP treatments in performance time. Exercise changes in rectal temperature, heart rate, Δ % plasma volume and plasma concentrations of total protein, free fatty acids, glucose, lactate, potassium, chloride, calcium, and sodium did not differ (P〉0.05) between trials. In contrast, blood hydrogen ion concentration [H+] was significantly lower (P〈0.05) at 30 min of exercise during the GP and EP treatment when compared with the NEP run. These data provide evidence that electrolyte drinks do not minimize exercise-induced disturbances in blood-electrolyte concentrations during heavy execrcise when compared with nonelectrolyte drinks; however, these results suggest that fluid replacement beverages containing buffers (i.e. C) and/or electrolytes may minimize blood alterations in [H+] during intense exercise. Additional research is required to determine if the buffering influence of these beverages has an ergogenic benefit during heavy exercise.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1439-6327
    Keywords: Exercise ; Induced hypoxemia ; Incremental exercise ; Trained athletes ; Arterial oxyhemoglobin saturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recent evidence suggests that exercise-induced hypoxemia (EIH) may occur in healthy trained endurance athletes. However, at present, no data exist to describe the regularity of EIH in athletes or non-athletes. Therefore, the purpose of the present investigation was to determine the incidence of EIH during exercise in healthy subjects varying in physical fitness. Subjects (N=68) performed an incremental cycle ergometer test to volitional fatigue with percent arterial oxyhemoglobin saturation (%SaO2) measured min-by-min. For the purpose of data analysis subjects were divided into three groups according to their level of physical training: 1) untrained (N=16), 2) moderately trained (N=27), and 3) elite highly trained endurance athletes (N=25). EIH was defined as a %SaO2 of ≤91% during exercise. EIH did not occur in any of the untrained subjects or the moderately trained subjects. However, EIH occurred in 52% of the highly trained endurance athletes tested and was highly reproducible (r=0.95; P〈0.05). These findings further confirm the existence of EIH in healthy highly trained endurance athletes and suggests a rather high incidence of EIH in this healthy population. Hence, it is important that the clinician or physiologist performing exercise testing in elite endurance athletes recognize that EIH can and does occur in the elite endurance athlete in the absence of lung disease.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 65 (1992), S. 37-42 
    ISSN: 1439-6327
    Keywords: Alveolar-arterialPO2 difference ; Pulmonary gas exchange ; VO2max ; Hypoxia ; Hyperoxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary These experiments examined the exercise-induced changes in pulmonary gas exchange in elite endurance athletes and tested the hypothesis that an inadequate hyperventilatory response might explain the large intersubject variability in arterial partial pressure of oxygen (P a02) during heavy exercise in this population. Twelve highly trained endurance cyclists [maximum oxygen consumption (VO2max) range = 65-77 ml·kg−1·min−1] performed a normoxic graded exercise test on a cycle ergometer toVO2max at sea level. During incremental exercise atVO2max 5 of the 12 subjects had ideal alveolar to arterial P02 gradients (P A-aO2) of above 5 kPa (range 5-5.7) and a decline from restingP aO2 (ΔP aO2) 2.4 kPa or above (range 2.4-2.7). In contrast, 4 subjects had a maximal exercise (P A-aO2) of 4.0-4.3 kPa with ΔP aO2 of 0.4-1.3 kPa while the remaining 3 subjects hadP A-aO2 of 4.3-5 kPa with ΔP aO2 between 1.7 and 2.0 kPa. The correlation between PAO2 andP aO2 atVO2max was 0.17. Further, the correlation between the ratio of ventilation to oxygen consumption VSP aO2 and arterial partial pressure of carbon dioxide VSP aO2 atVO2max was 0.17 and 0.34, respectively. These experiments demonstrate that heavy exercise results in significantly compromised pulmonary gas exchange in approximately 40% of the elite endurance athletes studied. These data do not support the hypothesis that the principal mechanism to explain this gas exchange failure is an inadequate hyperventilatory response.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 74 (1996), S. 391-396 
    ISSN: 1439-6327
    Keywords: β(2)-Adrenoceptor agonist ; Skeletal muscle ; Fiber type ; Myosin heavy chain isoforms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study examined the effects of 6 weeks of treatment with theβ (2)-adrenoceptor agonist, clenbuterol, on the soleus muscle of adult female Sprague-Dawley rats. Animals (4 months old) were divided into two groups: clenbuterol treated (CL,n=7) (2 mg kg−1 body mass injected subcutaneously every other day), and control (CON,n=7) (injected with isotonic saline). Post-treatment body weights were ≈ 5% greater in the CL group compared to CON (P〈0.05). Polyacrylamide gel electrophoresis (SDS-PAGE) of soleus myofibrillar protein indicated a clenbuterol-induced decrease (P〈0.05) in the relative percentage of type I myosin heavy chain (MHC) with a concomitant increase (P〈0.05) in type IIdx MHC, while the proportion of type IIa MHC was unaffected. ATPase fiber typing revealed increases (P〈0.05) in the proportion of type II fibers expressed both as a percentage of total fiber number and total cross-sectional area (CSA). Finally, mean type II fiber CSA was ≈25% greater (P〈0.05) in the CL groups as compared to the CON group. These data indicate that clenbuterol treatment results in alterations in the MHC phenotype and an increased proportion of type II fiber CSA in the soleus of adult rats. These observations were due to an increase in the total number of type II fibers, as well as hypertrophy of these fibers. Thus, the relative increase in the number of histochemically determined type II fibers and the emergence of the normally unexpressed type IIdx MHC isoform in the soleus suggest a clenbuterol-induced transition of muscle fiber phenotype as well as selective hypertrophy of the type 11 fibers.
    Type of Medium: Electronic Resource
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