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  • 1
    Electronic Resource
    Electronic Resource
    Influence of sodium chloride on the amount of CRF-granules and “classical” neurosecretory material (NSM) in the neurohypophysis of bilaterally adrenalectomized rats (1976)
    Springer
    Anatomy and embryology 149 (1976), S. 307-313 
    Details
    ISSN: 1432-0568
    Keywords: CRF-granules ; Sodium chloride ; Adrenalectomy ; Rat ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Bilaterally adrenalectomized female SPF Wistar rats were given tap water containing various concentrations of sodium chloride to drink ad lib. After a survival period of 14 days the animals were killed by perfusion-fixation. Their brains were removed, fixed in pieric acid formalin solution and embedded in Paraplast. Serial sections of median eminence and hypophysial posterior lobe were cut and stained with crotonaldehyde-fuchsine. In the stained sections the amount of CRF-granules visible in the external zone of the median eminence, and the amount of “classical” neurosecretory material (NSM) occuring in the internal zone of the median eminence and in the posterior lobe of the hypophysis were estimated. After administration of drinking solutions to which 0%–1% sodium chloride were added, the amount of CRF-granules increased with increasing NaCl uptake, whereas the amount of “classical” NSM was not changed. Application of tap water containing 2% NaCl led to a marked decrease in the amount of “classical” NSM but did only slightly impair the amount of CRF-granules. From the findings it is concluded that 1. following bilateral adrenalectomy administration of sodium chloride inhibits the secretion of CRF-granules; 2. CRF-granules and “classical” NSM, although histochemical identical, have different functional significances; 3. the mechanisms regulating the secretion of CRF-granules are different from those controlling the release of “classical” NSM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315446
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of adrenalectomy, total body X-irradiation and dexamethasone on the amount of CRF-Granules and “classical” neurosecretory material in the rat neurohypophysis (1975)
    Springer
    Anatomy and embryology 148 (1975), S. 267-278 
    Details
    ISSN: 1432-0568
    Keywords: Corticotropin releasing factor ; Neurosecretion ; Hypophysis ; X-irradiation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 195 female Wistar rats the influence of adrenalectomy, total body X-irradiation and dexamethasone treatment on the amount of CRF-granules in the external zone of the median eminence and “classical” neurosecretory material (NSM) in the internal zone of the median and in the posterior lobe of the hypophysis has been studied. In the normal rat only very few CRF-granules occur. 15 days after adrenalectomy increased amounts of CRF-granules but normal amounts of “classical” NSM are found. X-irradiation leads to a slight diminishment of “classical” NSM in normal rats. In adrenalectomized animals X-irradiation causes no changes in the amount of “classical” NSM but a marked decrease in the amount of CRF-granules. Application of dexamethasone from the 15th to 18th day after adrenalectomy enhances the augmentation of CRF-granules in irradiated and non-irradiated rats but does not influence the amount of “classical” NSM. The findings show that no correlation exists between the quantitative changes of CRF-granules and “classical” NSM under the experimental conditions used. Therefore it is to be assumed that the release of the two substances is regulated by different control mechanisms and that the substances have a different functional significance. The observations do not exclude the possibility that CRF-granules and “classical” NSM are biochemically identical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319847
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of adrenalectomy on “Gomori-positive” substances in the hypothalamo-neurohypophysial system of rats heterozygous and homozygous for hypothalamic diabetes insipidus (1977)
    Springer
    Anatomy and embryology 151 (1977), S. 81-89 
    Details
    ISSN: 1432-0568
    Keywords: Neurosecretion ; Adrenalectomy ; Diabetes insipidus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of adrenalectomy on the amount of “Gomori-positive” neurosecretory material in the outer layer of the median eminence and in the supraoptico-hypophysial system was studied in normal Long-Evans rats and in Long-Evans rats heterozygous and homozygous for hypothalamic diabetes insipidus. In all non-adrenalectomized rats very few “Gomori-positive” granules were found in the outer median eminence layer. After adrenalectomy the amount of the granules increased markedly in normal Long-Evans rats and only slightly in Long-Evans rats heterozygous for diabetes insipidus. However, no augmentation of the granules occurred in homozygous diabetes insipidus rats. The amount of “Gomori-positive” substances demonstrable in the supraoptic and paraventricular nuclei, in the inner layer of the median eminence, and in the neural lobe of the hypophysis was less in rats heterozygous for diabetes insipidus than in normal Long-Evans rats and the smallest in homozygous diabetes insipidus rats. No differences in the amounts of the substances were observed between adrenalectomized and non-adrenalectomized animals. The findings suggest that the “Gomori-positive” granules occurring in the outer layer of the median eminence of adrenalectomized rats are of similar origin as those of the supraoptico-hypophysial system and represent a vasopressin-neurophysin-complex. The results support the concept that vasopressin is involved in the regulation of ACTH release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315300
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  • 4
    Electronic Resource
    Electronic Resource
    Histochemical and immunohistochemical properties of the CRF-Granules and other „Gomori-positive” substances of the rat (1976)
    Springer
    Histochemistry and cell biology 46 (1976), S. 81-105 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In adrenalectomized rats, histochemical and immunohistochemical properties of the following secretion products have been investigated: 1. CRF-granules in the outer layer of the median eminence; 2. neurosecretory material (NSM) in the supraoptico-hypophysial system of the hypothalamus; 3. secretory granules in the TSH-cells of the anterior lobe of the hypophysis; 4. secretory granules in the ependymal cells of the subcommissural organ (SCO); 5. β-cell-granules in the islets of Langerhans in the pancreas. All these substances are characterized by their stainability with the so-called “Gomorimethod”. The experiments have included studies into: a) the extractability of the substances by various solvents; b) the digestability of the substances by pepsin or trypsin; c) their histochemically detectable content of disulfide groups, arginine and periodic acid-Schiff (PAS) reactive carbohydrates; d) their reaction with porcine-neurophysin-II-antibodies. All substances exhibited a positive reaction for disulfide groups. Based on their solutility properties, their resistance to pepsin or trypsin, their respective content of PAS-reactive carbohydrates and their failure to react with anti-neurophysin serum the “Gomori-positive” granules in TSH-, SCO- and pancreatic β-cells can be distinguished from one another and from CRF- and neurosecretory granules. In contrast, CRF-granules and NSM showed identical properties. Taking into consideration data from the biochemical and histochemical literature, the present findings suggest that CRF-granules and NSM consist of closely related biochemical substances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02462734
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of aldehydefuchsin-positive neurosecretory granules in the cells of the suprachiasmatic nucleus of the rat (1978)
    Springer
    Histochemistry and cell biology 58 (1978), S. 335-338 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Application of dark field microscopy to sections fixed with picric acid — formalin and stained with crotonaldehydefuchsin allows the demonstration of neurosecretory granules in the neurones of the suprachiasmatic nuclei of normal rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495390
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of fixation on the demonstration of neurophysin and “Gomori-positive” substances in neurosecretory granules of the rat hypothalamus (1977)
    Springer
    Histochemistry and cell biology 51 (1977), S. 327-333 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Proteins reacting with neurophysin antibodies and “Gomori-positive” substances were demonstrated histochemically in hypothalamic neurosecretory material of normal and bilaterally adrenalectomized rats after two different fixations: a) picric acid-formalin (PAF) for 7 days at 37°C; b) Bouin's fluid for 20 h at 4°C. After PAF-fixation anti-neurophysin reactive neurosecretory granules are found in all parts of the supraoptico-hypophysial system and in the suprachiasmatic nucleus of normal and adrenalectomized animals. In the latter they can additionally be demonstrated in the outer layer of the median eminence. Amount and distribution of “Gomori-positive” sustances correspond to those described for the immunoreactive material, except for the suprachiasmatic nucleus, in which the substances can not be detected. Following fixation in Bouin's fluid the immunohistochemical reactions are unchanged whereas the staining of “Gomori-positive” substances is remarkably impaired. The amounts of the substances demonstrable in the neural lobe are diminished and in the cells of the supraoptic and paraventricular nuclei as well as in both median eminence layers only traces of the substances are to be seen. The findings indicate that negative results in demonstrating “Gomori-positive” substances may be caused by inappropriate fixation and need to be controlled by immunohistochemical techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494369
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  • 7
    Electronic Resource
    Electronic Resource
    Distribution and properties of an adenosine triphosphatase in the tanycyte ependyma of the IIIrd ventricle of the rat (1976)
    Springer
    Histochemistry and cell biology 47 (1976), S. 145-157 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution, histochemical properties and ultrastructural localization of adenosine triphosphate (ATP) hydrolyzing enzymes in the tanycyte ependyma of the third ventricle have been studied in female Wistar rats. Using a calcium-cobalt procedure and a lead capture technique, splitting of ATP could be demonstrated in perikarya and processes of tanycytes in the region of the ventromedial nucleus. The reaction showed no dependence on magnesium or sodium ions, did not occur with other monodi-, and tri-phosphates as substrates, and was inhibited by p-chlormercuribenzoate (PCMB) and sodium fluoride, but not by ouabain. With the calciumcobalt method the highest intensity of reaction was found at pH 9.4, whereas the lead method gave optimal results at pH 6–8. At the ultrastructural level, the reaction product was found at the outer surface of the plasma membranes of tanycytes and reached its highest concentrations in the region of the apical microvilli. From the findings it is concluded that splitting of ATP in tanycytes is due to a true ATPase. The enzyme might be involved in an active transport of substances by tanycytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492562
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  • 8
    Electronic Resource
    Electronic Resource
    Buchbesprechungen (1979)
    Springer
    Naturwissenschaften 66 (1979), S. 118-120 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373513
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  • 9
    Electronic Resource
    Electronic Resource
    Buchbesprechungen (1978)
    Springer
    Naturwissenschaften 65 (1978), S. 496-500 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702850
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  • 10
    Electronic Resource
    Electronic Resource
    Neuroanatomical studies on corticotropin releasing factor (CRF) (1977)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 297 (1977), S. S57 
    Details
    ISSN: 1432-1912
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587779
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