Bibliothek

Notizen: Summary A comparative study was undertaken on the innervation of mucous and granular glands in frog skin. Results obtained by the Falck-Hillarp fluorescence technique and cholinesterase staining indicated that both types of glands receive exclusively adrenergic innervation. Electron microscopy was used to investigate the innervation pattern at the ultrastructural level. The distribution of nerve terminals was found to differ in the two types of glands. In the mucous gland, terminals were found at a distance of about 0.5 μm from the basement membrane but never within the gland parenchyma. In the granular gland, the terminals were located between smooth muscle cells and also in direct contact with the secretory epithelium but never outside the basement membrane.

Notizen: Summary Preliminary ultrastructural studies on the effects of 5,6-Dihydroxytryptamine (5,6-DHT) on the anterior byssus retractor muscle (ABRM) of Mytilus show degeneration of 2 types of monoaminergic nerves after 10 days of drug treatment. One type contained large granular vesicles (560–1,680 Å) while the other had small granular vesicles (200–640 Å). These axons may possibly represent serotonergic and dopaminergic nerves, thought to innervate this muscle. Two other types of profiles seemed to be unaffected by the drug. One conforms to cholinergic nerves while the other has a predominance of large opaque vesicles (1,200–2,500 Å). The significance of these findings is discussed in the light of recent observations on the neurotoxic effects of 5,6-DHT on vertebrate and molluscan nerves.

Notizen: Summary The unicellular hatching glands (UHGs) of four species of salmonid fish, Salmo gairdneri, Salmo trutta, Salvelinus fontinalis and Salvelinus pluvius were studied by light and electron microscopy. The UHGs are distributed on the epidermis of head and yolk sac, and on the epithelium of the mouth and gills. Since these cells are large and include dilated cisternae of the endoplasmic reticulum from the primitive to the mature stages they are conspicuous. Around the Golgi complexes, there are consecutive figures showing synthesis of secretory granules with close relationships to the Golgi vesicles. The secretory granules grow in size and vary in density during maturation; some have enclosed cytoplasmic structures. At the hatching stage, they are discharged with some cytoplasmic structures from the UHG which is located in the superficial layer of epithelium. After exhaustion of the secretory granules, the remainder of the contents of UHGs is liberated.

Notizen: Summary A multilamellate body (MLB), bearing close resemblance to an array of annulate lamellae, has been observed in several adenohypophysial cell types of the teleost, Hemihaplochromis philander. In longitudinal section, each MLB comprises a ladder-like row of 12–50 sausage-shaped profiles, termed lamellae. A few lamellae in each section show connections with the endoplasmic reticulum. Apposition of paired lamellar membranes at regularly spaced intervals results in a beaded appearance, whereby sites of membrane apposition are probable pore sites. The MLB differs from annulate lamellae in having poorly developed pores and closer packing of lamellae. It is suggested that the MLBs described here, may represent annulate lamellae at a stage of development or break-down when pores are incomplete.

Notizen: Summary Images have been obtained from freeze-etch replicas of neurohypophyses which are consistent with the view that orderly arranged aggregates of membrane particles occur in regions where fragments of membrane are being added to and taken away from the plasma membrane during secretion. Aggregates of particles included rosette-like and necklace-like patterns similar to those described by other authors at sites of exocytosis and endocytosis.

Notizen: Summary The fibers of drum and trunk muscles of the Tigerfish, Therapon jarbua, differ greatly in diameter. The myofibrils of the trunk muscles are irregularly oriented, while those of the drum muscles are rolled into spiral or concentric bands. Both muscle types possess the sarcomere structure typical of cross-striated musculature. However, the myofibrils of the drum muscles differ greatly in sarcomere length and width from those in the trunk musculature. The trunk muscles contain few mitochondria, whereas in the drum muscles mitochondria are abundant. The sarcoplasmic reticulum (SR) of the drum muscles takes the form of elongated tubes in both the A and the I region; that of the trunk musculature consists of small vesicles. Of the two muscle types, the drum muscle contains more SR. With respect to the form of the T system, the trunk musculature is of the Z type and the drum muscles of the A-I type. The drum muscle displays a considerably greater number of motor endplates; these lack typical junctional folds and have mitochondria with very few cristae. No fat could be demonstrated in either the drum or the trunk muscles. However, the concentration of glycogen is higher in the drum muscle than in the musculature of the trunk.

Notizen: Summary In ciliated cells of thymic cysts in Nude mice, ciliary rootlets are constantly and closely related to smooth endoplasmic reticulum and clear vesicles. This special association suggests that this structure does not play only an anchoring role but must be involved in the general metabolism of the cilium.

Notizen: Summary In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 μm diameter are present in the interphase nucleus. Granular nucleoli first appear at the 8-cell stage. These nucleoli have fibrillar and granular regions. The granular regions are made up predominantly of ribosome-like osmiophilic granules. From the 16 and 32-cell stage onwards, the one or two spherical nucleoli of each nucleus measure 2.5 μm in diameter and show a concentric organization with a very dense central region surrounded by a broad peripheral zone containing numerous granules, possibly of ribonucleoprotein. At the same time the number of ribosome-like particles increase in the karyoplasm and that of ribosomes in the cytoplasm. These findings are surprising because in eggs with radial cleavage, which have been subjected to more detailed analysis, the first granular nucleoli appear after the end of the cleavage, at the blastula or gastrula stage. The early appearance of granular nucleoli which seem to be characteristic of several eggs with spiral cleavage is discussed in connection with biochemical data on RNA-synthesis.

Notizen: Summary The fine structure of the endothelium lining the femoral and mesenteric veins in the rabbit was examined following the infusion of concentrations of adrenaline varying from 0.5 to 5.0 μg/kg. Control animals were infused with saline. Vesiculation of the endothelial cells was observed following each infusion of adrenaline, the effect being most marked with the highest dose used. It is suggested that this vesiculation may be associated with the release of antihaemophilic globulin and/or plasminogen activator from the endothelial cells.

Notizen: Summary The ultrastructure of coronet cells of the saccus vasculosus has been studied in specimens of Anguilla anguilla (L.) at different stages of its life cycle. At all the stages observed coronet cells are composed of a basal and an apical part, the latter bearing globules with primary vesicles. In the larva (a marine form) and in the fully metamorphosed small eel at the time of entry into freshwater the narrow lumen and the vesicles within the apical globules are filled with electron-dense material. In forms in which adaptation to freshwater has occurred, the saccus lumen appears expanded, the apical globules are better developed, and the electron-dense material has disappeared. It is suggested that the two situations observed represent different functional states of the organ, in relation to different conditions of environmental salinity.

Notizen: Summary 6-hydroxydopamine (6-OHDA) was shown to cause ultrastructural changes in adrenocortical cells of lizards and rats. These changes comprised the formation of dense bodies with lamellar and crystalloid patterns, a decrease in the number of mitochondria and structural alterations of mitochondria. Alterations in adrenocortical cells of lizards and rats differed in both qualitative and quantitative aspects. Adrenomedullary cells were not affected as a rule. Only in young animals did 6-OHDA cause deposits of an electrondense material in medullary cells. An attempt was made to obtain information on amine uptake into cortical cells using the Falck-Hillarp technique to analyse the in-vivo and in-vitro uptake of noradrenaline (NA) into the adrenal cortex in adult rats. Extraneuronal uptake into heart and spleen was studied as well. Our results suggest that NA is taken up into cortical cells, particularly into nuclei, after exposure to 10-4 gm/ml in-vitro indicating that uptake of 6-OHDA is also likely. Investigations using labelled 6-OHDA are required for further elucidating its extraneuronal uptake.

Notizen: Summary The ciliary body of the cat was investigated by fluorescence histochemistry and electron microscopy in an attempt to clarify its sympathetic innervation. Subconjunctival doses of 5-hydroxydopamine (5-OHDA) or 6-hydroxydopamine (6-OHDA) were given to establish the precise location of the sympathetic nerve terminals. The distribution of noradrenergic fibers and terminals was shown by fluorescence histochemistry to be sparse in the trabecular meshwork and the anterior portion of the ciliary muscle, but dense in the subepithelial tissue. The small and large dense core vesicles which occur in many nerve endings of the subepithelial tissue adjacent to the pigmented epithelial layer increased in electron density following the administration of 5-OHDA. Many degenerating nerve endings were found in the same region of animals treated with 6-OHDA. In contrast, there were few noradrenergic terminals in the ciliary muscle except for a portion of the smooth muscle which was shown to be dually innervated. The noradrenergic fibers in the subepithelial region and the trabecular meshwork may play an important role in aqueous secretion and outflow.

Notizen: Summary In the lamina ganglionaris, the first optic ganglion of the fly, the inventory of cell types as well as the patterns of their connections are well known from light microscopic investigations. Even the synaptic contacts are known with relative completeness. However, the structural details visible on electron micrographs are very difficult to interpret in functional terms. This paper concentrates on two aspects: 1) the synaptic complex between a retinula cell axon and four postsynaptic elements, arranged in a constant elongated array (it is suggested that all synapses in which the retinula cell is presynaptic are of this kind), and 2) the “gnarl” complex in which a presynaptic specialization in one neuron is separated from another neuron by a complicated glial invagination. The participation of glia at postsynaptic sites seems to be quite common in this ganglion. Occasionally it seems that a glia cell is the only postsynaptic partner facing a presynaptic specialization within a neuron.

Notizen: Summary Rabbit ovarian preovulatory follicles and in particular the overlying surface epithelium were studied by morphological and ultrahistochemical means at different times after mating. By light microscopy an increase of cytoplasmic granules was found in the surface epithelium at the follicle apex 4 h after mating. The granules increased in amount and showed maximal accumulation 8–9 h after mating. They then disappeared at the same time as the connective tissue elements in the underlying tunica albuginea and theca externa disintegrated. Transmission electron microscopy showed that the membrane-bounded granules or dense bodies fused with one another and by 8 h after mating they often changed character and appeared more electron lucent. Furthermore, open communications were found between altered granules and vacuoles and between vacuoles and the extracellular space below the epithelium. Acid phosphatase reaction product was localized to the granules and Golgi cisternae. Not all the dense bodies were enzyme positive. At later stages, close to the time of follicle rupture, the epithelial cells were attenuated and thin, with only a few granules. By scanning electron microscopy it was found that the epithelial cells at the follicle apex increased in size approaching the time of follicle rupture and that their microvilli decreased in number and in size. At 8 h and later, the contours of intracellular granules could be visualized. The results of this study were similar to those found when rabbits were induced to ovulate by HCG-stimulation. This further strengthens the hypothesis that the surface epithelium contributes proteolytic enzymes which help to disintegrate the follicle apex prior to rupture.

Notizen: Summary Using the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the electron microscopic level, it was demonstrated that the hormones of the posterior pituitary of Rana temporaria are located in separate vasotocinergic and mesotocinergic nerve fibres. This observation confirms the results of our previous immunocytochemical studies at the light microscopic level.

Notizen: Summary Epidermal septate junctions of Nereis sp. and Cirriformia sp. fixed with OsO4 or glutaraldehyde/OsO4 display variable structure in electron micrographs. In transverse section the septa are often indistinct and obscured by opaque material that fills the junctional cleft. Septa (spaced at 180–280 Å) are more clearly defined in slightly oblique transverse section; they exhibit an electron lucent center and appear to be linked by arms. En face views of the junction show a honeycomb pattern. Cytoplasmic faces of junctional membranes are backed with plaques opposite the septa. Lanthanum used as a tracer delineates junctional structure in negative contrast. In transverse section a chain-like lattice is present in the junctional cleft. En face views show parallel rows of pleated elements often linked by arms into honeycomb arrays. Oblique sections demonstrate that these pleated elements are continuous with the chain-like lattice seen in transverse sections. Lanthanum does not pass entirely through the junction. Lanthanum reveals that the septa have a very intricate substructure, but it is difficult to visualize the architecture that could generate the various images presented by these junctions when seen in different orientations. However, it is clear that these junctions possess some features that are diagnostic of several supposedly different types of septate junctions in invertebrates.

Notizen: Summary The effects of various concentrations of 6-hydroxydopamine (6OHDA) on rat adrenocortical cells in tissue culture were studied with phase contrast and electron microscopy. With 40 mg/l of 6-OHDA the first signs of alteration as revealed by microcinematography appeared in isolated cortical cells as early as 15 min after addition of the drug. There was a cessation of movement of cell organelles and an immobilisation of membrane undulations followed by the development of dark inclusion bodies. The cells underwent increasing shrinkage and collapsed by 11/2 h. Chromaffin cells were not affected until 45 min after exposure to the drug and neurons were the most resistant population. However 61/2 h after application of the drug most cells in the culture were dead. 6-OHDA applied in different doses and to adrenal expiants did not alter the sequence of events. Ultrastructurally cortex cells underwent damage along two lines: they either showed lytic changes or developed various types of dense bodies before reaching the lytic stage. Treatment of cortical cells with 40 mg/l 5-or 6-OHDA followed by exposure to buffered 2% glyoxylic acid and heat did not produce a fluorescence within the cells. Microspectrofluorimetry on amine models with noradrenaline, 5- and 6-OHDA revealed that neither 5-nor 6-OHDA are capable to form a fluorophore with glyoxylic acid.

Notizen: Summary Electron microscopy of testicular biopsies obtained from two adult males with tunica vaginal hydrocele revealed some protrusions from the basal lamina to the germinal epithelium in the seminiferous tubule. The protrusions were of three types: some between the spermatogonia and Sertoli cells, some directly within the Sertoli cell cytoplasm and others inside the spermatogonia. The protrusions inside the spermatogonia were only 0.5 μm deep whereas the other types were from 1–11 μm deep. Occasionally some cut off portions of these protrusions were seen inside the ground cytoplasm of the Sertoli cell without an apparent connection with the original stalk. The matrix of the protrusions contained a homogenous component (composed of a fine filamentous element) and granular and membranous components. These components closely resemble the materials found in the basal lamina of the seminiferous tubule. It has been suggested that under mild pathological conditions, i.e., hydrocele, the junctions between the seminiferous tubule epithelium and the basal lamina become somewhat more flexible. As a result, the protrusions become longer and a passage might be formed to allow the flow of raw materials in or out of the seminiferous tubule.

Notizen: Summary The structure of the nongranulated cells in the sea lamprey adenohypophysis and similar cells of the rostral pars distalis in a number of actinopterygian fishes was examined with the aim of determining the role(s) of these cells in pituitary function. A number of possible roles are proposed for the nongranulated cells. In salmonids and Amia calva the cells may be involved in the active movement of material into or from the follicle lumina. The structure of the nongranulated cells in in vitro cultured and in in vivo transplanted trout rostral pars distalis also suggests a phagocytotic role for these cells. In teleosts with a non-follicular rostral pars distalis the nongranulated cells appear to play roles in the release of granules from the prolactin cells and in the subsequent dispersal of the hormone (and/or carrier substance) into the peripheral circulation.

Notizen: Summary The problem of the direct retinohypothalamic projection in mammals (Moore, 1973) was reinvestigated in the laboratory mouse by electron microscopy and cobalt chloride-iontophoresis. The time-course of the axonal degeneration in the suprachiasmatic nucleus was studied 3, 6 and 12 h, 1, 2, 4, 6, 9 and 12 days after unilateral retinectomy. Specificity of the degenerative changes was controlled by investigation of the superficial layers of the superior colliculus. The ratio of crossed to uncrossed optic fibers could be determined by counting degenerating structures (axons and terminals) in the optic chiasma and the ipsilateral and contralateral areas of the optic tract, the suprachiasmatic nucleus, and the superior colliculus. The number of degenerating axons in the suprachiasmatic nucleus showed a maximum one day after unilateral retinectomy and was, at all stages studied, two to three times higher in the contralateral than in the ipsilateral nuclear area. In the optic tract and in the superior colliculus the number of degenerating profiles was three times higher in the contralateral than in the ipsilateral area. Retinohypothalamic connections and crossing pattern of retinal fibers were studied light microscopically using impregnation with cobalt sulfide in whole mounts of brains. Most of the optic fibers in the laboratory mouse are crossed (70–80%). A bundle of predominantly crossed optic fibers runs to the suprachiasmatic nucleus.

Notizen: Summary Tissue from the digitiform rectal gland of the spiny dogfish, Squalus acanthias, was fixed briefly by formaldehyde perfusion and studied for the specificity and localization of p-nitrophenyl phosphatase (NPP'ase) activity. The enzymatic activity was K+-dependent (56%) and ouabain-sensitive (67% inhibition). The electron-dense reaction product (SrPO4) of the cytochemical reaction (Ernst, 1972b) was localized along the inner surfaces of the basolateral membranes of the secretory cells. It was absent from mitochondria, nuclei, vesicles, and other organelles. The luminal surface of the secretory cells was slightly reactive. On the basis of (1) this pattern of localization for the sodium transport system, (2) the presence of extensive intercellular labyrinthine channels (Bulger, 1963) that would facilitate “standing gradients” (Diamond and Bossert, 1968), and (3) the specific distribution of the energy-providing mitochondria, we conclude that the concentration and electrochemical gradients recorded from the secreting gland (Hayslett et al., 1974) are maintained across the domains of the basolateral surfaces of the secretory cells.

Notizen: Summary The ultrastructure of human placental capillaries was investigated using perfusion fixation and the freeze-fracturing technique. The capillaries have a continuous endothelium especially rich in microfilaments, whereas micropinocytotic vesicles are exceedingly scarce. The endothelial cells are connected by three types of junctions: (1) zonulae occludentes characterized by 2 to 4 focal regions of membrane contact in thin-sectioned specimens and an equal number of ridges on the membrane E-face in freeze-fractured specimens; (2) small gap junctions associated with the zonula occludens. (3) attachment plaques resembling zonulae adhaerentes in their fine structure. Endothelial cells are provided with long, circularly oriented pseudopodial extensions, which may be responsible for intermittent constrictions of the vessel lumen. These findings indicate that diaplacental transport at the level of the fetal capillary is controlled by the cytoplasm of the endothelial cells and probably occurs only to a very limited extent by way of micropinocytotic vesicles.

Notizen: Summary In the rat superior cervical and coeliac-mesenteric ganglia we have observed three types of small granulated (SG) cell: Type I cells are characterised by membrane-bounded cytoplasmic granules with a core of variable, moderate to low electron-density, whose limiting membranes are rounded in profile ranging from 50–150 nm in diameter. Type II SG cells contain numerous highly electron-dense, polymorphic cytoplasmic granules ranging from 100–300 nm in diameter. The haloes of Type II cell granules are variable in shape, and the core is often eccentrically located or fragmented. Type III SG cells contain membrane-bounded granules with a core of variable moderate to low electron-density. In profile these granules appear oblong or circular with average dimensions of 170 × 50 nm. All three SG cell types receive cholinergic-type pre-ganglionic terminals whose afferent nature is confirmed by their degeneration following pre-ganglionic neurectomy. Only Type I cells have been observed to donate efferent synapses to dendrites of principal ganglionic neurones and are thus interneuronal.

Notizen: Summary The sinu-atrial node in the heart of the mole, Mogera wogera, contains myocytes which are devoid of atrial specific granules and which may be classified into two types: electron-lucent (majority) and dark (minority). Numerous unmyelinated axon terminals, containing synaptic vesicles, face the nodal myocyte surface with interspaces of less than 300 nm. There are about 6 terminals per myocyte profile and about 10 per profile at nuclear levels. Of a total of 2717 of these terminals 85.2% are 50–200 nm from the nodal myocytes, and 9.6% form closer neuromuscular junctions, with less than 50 nm interspace and some membrane specialization. Such specializations are almost exclusively found in relation to the dark, minority-type of nodal myocyte. Myelinated nerve fibers are also numerous within the mole SA node, and may terminate almost directly on a nodal myocyte. A few nerve fibers contain many mitochondria and may represent afferent endings. Nodal capillaries are smaller and fewer than capillaries in the juxtanodal myocardium, probably indicating a lesser blood supply to the SA node than to the ordinary myocardium.

Notizen: Summary The duodenum of 16-day Black Australorp chick embryos, and the duodenum, ileum, large intestine and caeca of 18-day embryos and of chicks within 30 h of hatching, have been studied by electron microscopy. Cells were found with secretory granules resembling those in mammalian EC, S, A-like, EG and D cells (terminology of Solcia et al., 1973), and were on this basis tentatively identified accordingly. The distribution and frequency of the chick cells in different parts of the tract correspond well to the situation in mammals.

Notizen: Summary The proventriculus, gizzard and pyloric antrum (region between the gizzard and the duodenum) of 18-day Black Australorp chick embryos and of chicks within 30 h of hatching have been studied by electron microscopy. D and EC cells, and putative G, D1 and A-like cells were identified (terminology of Solcia et al., 1973) but no ECL cells. No endocrine cells of any kind were revealed in the gizzard.

Notizen: Summary Reflecting chromatophores in the integument of the guppy, Lebistes reticulatus Peters, are of two distinct types, iridophores and leucophores. The iridophores are smaller and fixed, producing a metallic iridescent color. The cytoplasmic organelles involved in the coloration of iridophores are the reflecting platelets, as in the iridophores of other fish and amphibian species on which earlier reports have been made. Spherical granules of pleiomorphic internal structure, quite variable in size but generally 0.2 μm to 1.0 μm in diameter, are also numerous in the iridophores. The nature of these granules remains unknown. The leucophores are larger, and highly dendritic; their pigment granules are migratory and they exhibit a dull whitish color. Pigment granules of the leucophores are spherical in form, varying from 0.5–0.8 μm in diameter, with a double membrane enclosing the internal fibrous materials. Melamine-treatment of the fish caused degenerative changes in the pigment granules and also the other cytoplasmic organelles of the leucophores, whereas the other kinds of chromatophores, including the iridiophores, remained intact. Some problems in general characterization and classification between these two types of chromatophores were discussed.

Notizen: Summary Neither the concept of the Reticulo-Endothelial-System (RES) Aschoff's (1924) nor that of the Reticulo-Histiocyte-System (RHS) provides a satisfactory framework into which the present knowledge of the phagocytic mononuclear cells can be fitted. Current knowledge concerning morphology, histochemistry (peroxydase and esterase activity), immunology (specific surface antigens, receptors on the cell membranes), function (immune phagocytosis, pinocytosis), kinetics (3H-thymidine labelling) and culture makes it possible to place all highly phagocytic mononuclear cells and their precursors in one system, which is called the Mononuclear-Phagocytic-System (MPS) (Langevoort, Conn, Hirsch, Humphrey, Spector, van Furth, 1969). Kinetic studies with labelled cells have shown, that mononuclear phagocytes originate from precursor cells in the bone marrow (stem cell → monoblasts → promonocytes), than are circulating in the peripheral blood as monocytes and are transformed to tissue macrophages entering tissues. The MPS comprises following cells in following organs: connective tissue (histiocytes resp. macrophages); liver (Kupffer-cells); lung (alveolar macrophages); lymph nodes (free and fixed macrophages); bone marrow (macrophages); serous cavities (pleural and peritoneal macrophages); bone tissue (osteoclasts?); nervous system (microglial cells) (see Table 1). The reticular cells, endothelial cells and fibroblasts (fibrocytes) can therefore not be included in the MPS. Besides differences in morphology, histochemistry and function, they derive from mesenchymal cells and not from the bone marrow as the MPS. The present investigation demonstrates the structure and significance of the MPS in various kinds of chronic-specific and non-specific rhinosinusitis. On semithin sections two kinds of macrophages can be distinguished light-microscopically: 1. Larger macrophages with many phagosomes (storage cells) (Fig. 1A), which can exhibit sometimes a ring-shape on sections embracing greater parts of the interstitium (Fig. 1B). Such forms are mainly found in chronic (maxillary) sinusitis and are interpretated as “scavenger” macrophages. 2. The second type consists of smaller macrophages with extremely ruffling of the cell surface, wich is interpretated as an expression of highly (specific?) stimulated states. These later macrophages can be seen mainly in edematous nasal polyps, which might be caused by allergic reactions of the anaphylactic type. The fine structure of the phagocytes is to some extend dependent on the actual development and functional state: there are “immature” macrophages, which are practically indistinguishable from blood monocytes (Fig. 2A); some of them can be stimulated and can therefore show many surface foldings and projections (Fig. 2B). The “mature” macrophage shows a well developped Golgi-area and many secondary lysosomes (Fig. 3). The storage type of the macrophages, which can predominate in some cases of chronic maxillary sinusitis, is characterized by many electron-lucent vacuoles (Fig. 4). The macrophages can transform unspecifically to epitheloid- and giant-cells, as it is shown in foreign bodys- and cholesterol-granulomas in maxillary sinusitis (Fig. 5A and 5B). This tranformation to epitheloid and giant-cells might also be induced specifically by stimulated T-lymphocytes in immunological reaction of the delayed type, for example in sarcoidosis (Fig. 6A) and tuberculosis (Fig. 6B) of the nose. The macrophage is not only of great importance as the carrier of the cellular immunity in the sense of Metschnikoff (1905), but he is also involved in the initiation and regulation of the specific humoral and cellular immune responses. These facts and the phagocytic and synthetic properties of the macrophages are discussed in regard to their significance for the etiology and course of chronic rhinosinusitis.

Notizen: A method is presented to calculate the eigenvalues of the Helmholtz equation Δ2ø + k2ø = 0 in a two-dimensional area when ø vanishes on the boundary. The method is based on an integral equation, which can be easily solved numerically. Results obtained for circular and rectangular geometries are also given and compared to the exact values.

Notizen: This paper presents the results of an aeroelastic optimization study. In this study the weight of a large aspect ratio panel immersed in high Mach number supersonic flow is minimized subject to the requirement that a critical aeroelastic parameter for flutter be held within specified limits. A refined finite element technique is used to model the panel flutter equilibrium equations, equations which act as constraints on the design search. The optimization mechanism itself is studied and discussed to provide qualitative results which may be useful to the solution of other aeroelastic optimization problems. The numerical results of this investigation are compared to converged numerical results to illustrate finite element accuracy. The results of the study show that the weight savings and material distribution found with an effective finite element model are comparable to those found by more complicated numerical methods.

Notizen: A computer solution for two-dimensional fluid-particle flows using the cellular approach is presented. The euqations describing the flow of the continuous (fluid) phase are formulated using stream function, vorticity and enthalpy as the dependent variables. The effect of the disperse (particle) phase on the continous phase is represented by vorticity and energy sources in each cell. To illustrate the capability of the solution scheme, the flow of gas laden with hot particles issuing into a sudden expansion is analysed.

Notizen: This paper presents the optimum design of stiffened cylindrical panels with weight as the objective function and constraints on the frequencies in the presence of initial stresses, using unconstrained minimization techniques of non-linear mathematical programming problem. The interaction between the buckling constraints and the frequency constraints in the presence of initial stresses are included in the formulation. Loss of load carrying capacity due to imperfection and due to suddenly applied loads are included in the buckling analyses. Results for cylindrical shell are obtained by setting the panel angle to 360 degrees. The relationship between the weight of the cylinder and the panel (a segment of the cylinder) is examined.

Notizen: The solutions of plane elasto-plastic problems usually use one or two field variables - namely a stress functions or the displacements. The use of three, four or five field variables is investigated and it is concluded that the three stresses from the best basis for a multivariable approach. Attempts to solve the governing equations with an initial value technique were exhaustively tried and discarded in preference to a boundary value or elliptical technique. The problem solved to check the method is that of a hole in plane strain uniaxial tension. Effective plastic strain distributions are plotted, along with stress and strain concentration data and distributions of the mean to effective stress ratio. Comparison is made between solutions produced using incremental and deformation theories.

Notizen: Finite element procedures and related illustrative numerical examples for incompressible viscous fluid motion are discussed in this paper. The steady flow problem is solved by the Newton-Raphson method and the perturbation method. By numerical examples, it can be shown that the combined use of the Newton-Raphson method and perturbation method is suitable. For the analysis of unsteady flow, the perturbation method is employed. Assuming that the basic flow is known, unsteady flow is calculated by accumulating the solution of the linearized equation in which the boundary values are varied by small amounts. Steady flows of temperature dependent free convection are also discretized and analyzed by the same procedure as the conventional finite element Galerkin method. For shape functions, quadratic polynomials are used for velocity and temperature, and linear polynomials for pressure. It is to be noted that the selections of shape functions and solution method are the keys to the analysis of highly non-linear fluid flow problems such as those discussed in this paper.

Notizen: A procedure of applying the perturbation method is presented for the incremental numerical analysis of materially and combined non-linear problems of discrete and discretized structral systems. Small but finite strain and stress increments are strictly distinguished from the strain rates and stress rates, respectively. It is shown that, by applying the perturbation procedure not only to the non-linear strain-displacement relations and equilibrium equations, but also to the constitutive equations in terms of rate quantities, all the governing equations can be satisfied to any desired accuracy at every instantaneous configuration in between the starting and terminal points of an incremental step. The proposed method provides also means of finding, to a desired accuracy, every point on an equilibrium path of a discrete system at which a new element will start yielding or unloading and possible critical points on the path. The significance of the proposed method is expected to be appreciated particularly in numerical investigations of critical behaviours and post-buckling behaviours.

Notizen: This paper is concerned with the development of a numerical procedure for solving complex boundary value problems in plane elastostatics. This procedure - the displacement discontinuity method - consists simply of placing N displacement discontinuities of unknown magnitude along the boundaries of the region to be analyzed, then setting up and solving a system of algebraic equations to find the discontinuity values that produce prescribed boundary tractions or displacements. The displacement discontinuity method is in some respects similar to integral equation or ‘influence function’ techniques, and contrasts with finite difference and finite element procedures in that approximations are made only on the boundary contours, and not in the field. The method is illustrated by comparing computed results with the analytical solutions of two boundary value problems: a circular disc subjected to diametral compression, and a circular hole in an infinite plate under a uniaxial stress field. In both cases the numerical results are in excellent agreement with the exact solutions.

Notizen: The application of the finite element method to the supersonic flutter of circular cylindrical shells subjected to internal pressure and axial compression is presented. A circular cylindrical shell element is used. The element stiffness, mass and initial stiffness matrices are given. The element aerodynamic matrix is derived based on a first order high Mach number approximation to the linear potential flow theory. The eigenvalue problem is solved by the QR algorithm. Numerical results are presented and these are compared with analytical solutions and experimental data.

Notizen: A straightforward and general computer program for assembling and solving (using Gauss elimination technique) widely sparsed finite element matrix equations with very large bandwidth and capable of handling different degrees-of-freedom and variable bandwidth at different nodes, is described herein. The program assembles any type of finite elements having arbitrary number of nodes and each node may have differnt degrees-of-freedom. It requires only a small core memory in the computer, although a fast random access device is also needed. The two very important features of this program are (i) it does not store any zero submatrices within the band and (ii) during the solution of equations all operations dealing with zero submatrices within the band are automatically skipped and thus the savings of a considerable amount of disc storage space and computer time can be effected in many cases. Another feature is that many right hand sides can be handled simultaneously. Hence the program is very economical for structures having widely sparsed matrix equations. A listing of the computer program written in FORTRAN IV for CDC 6400 computer is readily available from the authors, but unfortunately could not be given here because of lack of space. The program is so general that it can be used to solve a wide class of finite element problems without actually having to understand fully the techniques behind it.

Notizen: The Peaceman-Rachford ADI technique is a well-known iterative method for solving large systems of linear equations arising from finite difference methods of solution of partial differntial equations. The present note demonstrates how the algorithm can be conveniently rearranged for computation in a virtual storage system with paging. Timing results are given for a sample problem typical of those encountered in nuclear diffusion computation.

Notizen: Alternative finite difference formulations for elliptic equations on skew nets are investigated for suitability for rapid convergence of S.O.R. iterations. In dealing with the differential equation directly, diagonal dominance is a key property for convergence theorems. Mixed derivatives boundary conditions threatens this property, but formulas are presented which protect it as long as the net skewness is not too severe. Test calculations are reported showing convergence for these formulas to be as good as that of optimal S.O.R. theory, whereas other more direct formulas give a very inferior performance.

Notizen: A frontal solution program is presented which may be used for the solution of unsymmetric matrix equations arising in certain applications of the finite element method to boundary value problems. Based on the Gaussian elimination algorithm, it has advantages over band matrix methods in that core requirements and computation times may be considerably reduced; furthermore numbering of the finite element mesh may be completed in an arbitrary manner. The program is written in FORTRAN and a glossary of terms is provided.

Notizen: Quadratic isoparametric elements which embody the inverse square root singularity are used in the calculation of stress intensity factors of elastic fracture mechanics. Examples of the plane eight noded isoparametric element show that it has the same singularity as other special crack tip elements, and still includes the constant strain and rigid body motion modes. Application to three-dimensional analysis is also explored. Stress intensity factors are calculated for mechanical and thermal loads for a number of plane strain and three-dimensional problems.

Notizen: The equations for the meridional through-flow in a turbomachine are formulated as a quasi-harmonic non-linear equation. This equation is then solved iteratively by the finite element method and in order to obtain convergence an under-relaxation factor has to be introduced. Comparisons with experimental results in single and multistage axial compressors show very good agreement. The finite element method is thus shown to be an efficient tool for this type of problem.

Notizen: Finite element methods for solving the two-dimensional x-y transport equation are considered. Numerical schemes are defined and tested on some simple practical problems.

Notizen: The problems associated with the stress analysis of structures subjected to body force loading due to compound motion are outlined. An economic method for calculating equivalent nodal loading, for use in a finite element displacement analysis, is proposed in which the element discretization and consistent mass matrices are used to advantage. The relevant equations, relating to the rigid body dynamics of compound motion, are appended in matrix notation. Validity of the method is demonstrated by an analysis of the stresses, due to gyroscopic and centrifugal forces, in an aero engine blade model.

Notizen: An automatic algorithm is presented for reducing the bandwidth of symmetric sparse connectivity matrices. The procedure takes an arbitrary input sequence and creates an optimal bandwidth for complex network systems by renumbering the node labels. Thus, considerable improvement for computer storage and time is achieved in the solution of the systems.A large number of examples is presented to demonstrate the reliability and the efficiency of the method.

Notizen: An accurate reduction scheme for structural eigenvalue problems is deduced from a variational theorem in which the displacement, velocity and/or momentum fields are taken to be independent.

Notizen: This paper compares three methods for dealing with an exterior boundary value problem by the Finite Element Method, one of which involves using an infinite element. The methods are illustrated by application to the problem of ground water flow round a tunnel with permeable invert. The use of a special trial function with a variable parameter in the infinite element gives a particularly efficient method of solution.

Notizen: Bathe's algorithm of subspace iteration for the solution of the eigenvalue problem with symmetric matrices is improved by incorporating an acceleration technique using Chebyshev polynomials. This method of acceleration is particularly effective for this kind of iteration. The rate of convergence of the iteration scheme presented is considerably improved when compared with the original one, and satisfactory rates of convergence can be obtained for a wider range of eigenvalues.

Notizen: It is shown that an apparently sound selection of trial functions can give rise to hybrid elements with rank deficient stiffness matrices and unused interior fields. An account is given of modifications which overcome these problems for a particular plate bending element.

Notizen: The shape of a plate in plane stress is determined, such that the maximum elastic stress corresponding to given loads is minimized. The shape of the boundary is approximated by a series and optimization is carried out by solving a sequence of linearized minimum-maximum problems using linear programming. The optimization problem is extended to include multiple loading cases and geometrical constraints. The stress derivatives are found using analytical expressions for stiffnesses and stiffness derivatives of the finite elements. For the optimum design of a hole in an infinite plate under biaxial stretching the numerical result is compared with an analytical solution. As another example the method is used to optimize the edge shape of a shape of a junction in a web frame.

Notizen: Continuous (integral) and discrete (point-matching) least-squares methods are presented for linear and non-linear problems in boundary-value, eigenvalue, and initial-value form. The history is traced, and important theoretical and practical results are summarized. A comprehensive sample of the literature is presented, indexed to show type of application, version of least squares used, and results of comparison studies. The advantages of least-squares methods are discussed, including convenience in formulation and error evaluation, generality of mixed and local (finite element) versions, and performance that is competitive with other methods.

Notizen: An algorithm is presented which generates an element stiffness matrix for non-prismatic beam-column members using Newmark's numerical procedure of successive approximations. The resulting element stiffness matrix on element co-ordinates is fond to be in excellent agreement with those available for limiting cases. An approximate stability analysis conducted on the covergence of the numerical scheme shows that the proposed algorithm is stable. The critical buckling loads for various and conditions are computed as part of the computational scheme. A computer program listing in FORTRAN IV is included which will handle essentially and order and/or kind of non-prismaticity of beam elements. The results are presented in the conventional and convenient coefficient format.

Notizen: It is first shown that the two-dimensional linearized ship wave problem can be recast as the sum of a radiation and a diffraction problem for simple harmonic waves. Each problem can be solved by a hybrid element method (HEM) where conventional finite elements are used near the body and analytical solutions are used in the remaining infinite regions (super-elements). Variational principles which incorporate the matching conditions between regular and super-elements as natural conditions are derived. Numerical examples are presented. The theoretical aspects for extending the above ideas to a three-dimensional ship wave problems are also described.

Notizen: The quadrature rule for a finite interval obtained by transforming to an infinite range and applying the midpoint rule is examined. It is found that the condition required to make the quadrature exact for a constant integrand imposes severe practical limits. The method is very good for integrands with singularities near the endpoints but ineffective for oscillatory integrands.

Notizen: The contouring of stresses in two-dimensional slopes is done quickly and efficiently by using the computer and an incremental plotter. The computer program developed here interpolates in two directions between centroids of finite elements at which the stresses are given, determines contour line co-ordinates, and plots the contours. This program can be easily modified to plot stresses produced by other two-dimensional finite element analysis programs as long as the arrangement of elements in the mesh is similar.

Notizen: A conformable displacement model is presented for the finite element analysis of stress intensity factors in linear elastic fracture mechanics. The method is an extension to anisotropic materials of the one developed by Benzley for isotropic materials. Very good agreement is shown for the case of inclined cracks in an orthotropic plate between finite element results and those obtained by least squares collocation of stress functions.

Notizen: A numerical method for integrating the three-dimensional Navier-Stokes equations for a viscous, incompressible, laminar duct flow is given. A new method is applied to the derivation of stable, and second order accurate difference representations to the momentum transport and diffusion terms. Numerical examples are given.

Notizen: An elasto-plastic plate-bending analysis is developed based on the finite element method and utilizing an assumed-stress hybrid (Pian) element. Plasticity is incorporated using the Prandtl-Reuss relations and isotropic hardening theory together with an incremental residual force technique. Several example solutions are included to demonstrate the ability of this approach in elasto-plastic plate-bending problems.

Notizen: A finite element procedure based on residual potential or flow concept is proposed for free surface flow through porous media. A preliminary example is solved by using the proposed procedure.

Notizen: The convergence of Hankel's asymptotic expansions for the Bessel functions of large complex argument are discussed, and a method is proposed to avoid numerical problems derived from their implementation in computer programs. Whiel the error tolerance cannot in all cases be controlled by the user, excellent results can indeed be obtained with application of the proposed method.

Notizen: Some new rational approximations to the error function erf(x) are discussed. They have the same asymptotic behaviour as erf(x), and are more accurate than the ones previously published.

Notizen: An algorithm for computing a linear Chebyshev approximation to a function defined on a finite set of points is presented. The method requires the accuracy of the approximation to be specified, and determines the least degree approximation which achieves this accuracy. The algorithm is based upon the simplex method of linear programming. A FORTRAN program is supplied in the Appendix.

Notizen: The classical concept of slip-surfaces in soil mechanics is assigned a physical significance based on frequent observations of rupture in overconsolidated soils and rock masses; propagation of a narrow shear-band (i.e. slip-surface) is commonly the mechanism of failure. Effectively, relative sliding occurs between adjacent material points on the surface, a concept familiar in rack mechanics; just as failure by crack propagation can occur in metals well below net section yielding, so can progressive failure by band propagation cause slope failure at nominal stresses well below the peak strength of the material. The relative sliding is simulated here, for a material response which is linear outside the band, by superposing continuously distributed dislocations; use is made of influence functions, for dislocation and point-force singularities in a variety of materials and geometries. The method has further application in rock mechanics and metallurgy. A stress-displacement constitutive relation is imposed on the line of the band and the resulting non-linear singular integral equations necessitate numerical solutions; the routines are prefected on two problems for which answers are otherwise available. Extension to previously intractable plane-strain problems is then described and results are given for a surface-parallel shear-band corresponding to a flake slide on a long slope.

Notizen: The transmission-line matrix method is a time-domain numerical method for solving wave problems. This paper describes how a minor change in the computer program for loss-free dielectrics extends the method to include dielectric losses. Results are given in terms of the wave impedance of a waveguide with losses, and also in terms of power decay due to losses in the dielectric of a resonant cavity.

Notizen: A procedure for numerical quadrature over a semi-infinite range is described which does not require storing weights or nodes. It is applicable to a wide variety of integrands including oscillatory functions and forms with integrable singularites. A computer program and numerical examples are presented.

Notizen: It is possible to transform elliptic partial differential equations to exchange the dependent with one of the independent variables. The Laplace equation for a stream function ‘Ψ’ over the X and Y co-ordinate system, for example, can be transformed into a relationship expressing the Y position of streamlines in terms of strem function Ψ and X.Although the resulting new partial differential equation is much more complex it is much more convenient to use in a computer. An irregular Y boundary becomes, with the new relationship, merely the boundary values assigned to the outer streamlines and the computer always need only deal with a rectangular array. The resulting answer is in the form of the position of streamlines which is the information directly required for plotting flow maps.

Notizen: An elasto-plastic analysis of anisotropic work-hardening materials, using the finite element method is presented. The analysis is based on the generalized Huber-Mises yield criterion extended by Hill for anisotropic materials. General expressions for the anisotropic parameters in the yield criterion have been derived both for initial yielding as well as subsequent yielding in the case of work-hardening materials.The isoparametric ‘quadratic’ quadrilateral elements have been used for the analysis and the ‘initial stress technique’ has been adopted for the iterative solution of the non-linear problems. The results of the various numerical examples have been compared with the available solutions.

Notizen: An automated digital computer procedure is presented in this paper which enables efficient solution of the eigenvalue problem associated with the supersonic panel flutter phenomena. The step-by-step incremental solution procedure is based on an inverse iteration technique which effectively utilizes solution results from the previous step in determining such results during the current solution step. Also, the computations are limited to the determination of a few specific roots only, which are expected to contain the flutter mode, and this is achieved at each step without having to compute any other root.The structural discretization achieved by the finite element method yields highly banded stiffness, mass, and aerodynamic matrices; the aerodynamic matrix evaluated by the linearized piston theory is real but unsymmetric in nature. The solution algorithm presented in this paper fully exploits the banded form of the associated matrices, and the resulting computer program written in FORTRAN V for the JPL UNIVAC 1108 computer proves to be most efficient and economical when compared to existing procedures of such analysis. Numerical results are presented for a two-dimensional panel flutter problem.

Notizen: The described program PFVIBAT uses the exact displacement method to perform free and forced vibration analysis entirely within the differential equation theory of beams thus avoiding assumed modes and lumped masses. The frame may contain rigid bodies. Clamped, hinged, guided and rolling connections are allowed for. Consideration of rotatory inertia, shear deformation and second-order bending moments and shear forces as caused by static axial load is optional. Eigenfrequencies and modal masses are calculated with an accuracy that may be specified. Displacement and moment modes are plotted. Transient vibrations are studied.

Notizen: ADI difference schemes of two and three levels for the parabolic equations with a mixed derivative have been derived. Using the stability analysis and the principal part of the truncation error, a difference formula with minimum truncation error has been found. Comparative study of the two and three level formulas has been made through two examples, one with constant coefficients and another with variable coefficients.

Notizen: One of the integral parts of any finite element procedure is the subdivision of a finite dimensional region into a finite number of non-overlapping subregions. An algorithm for subdivision of 2-dimensional polygons into triangular subregions is presented here. The algorithm consists conceptually of two steps. In the first step the region is subdivided into convex subregions; In the second step the convex subregions are further subdivided into triangular elements.

Notizen: Galerkin finite element methods based on symmetric pyramid basis functions give poor accuracy when applied to second order elliptic equations with large coefficients of the first order terms. This is particularly so when the mesh size is such that oscillations are present in the numerical solution. In the present note asymmetric linear and quadratic basis functions are introduced and shown to overcome this difficulty in an appropriate two point boundary value problem. In particular symmetric quadratic basis functions are oscillation free and highly accurate for a working range of mesh sizes.

Notizen: Generalized quadrature is used for the numerical solution of two Fredholm integral equations which occur in electrostatics and aerodynamics.

Notizen: A procedure is given for generating two-dimensional conforming singularity elements from standard conforming elements. Three new elements with 0(r-p) derivative singularities are introduced. The technique is based on the use of elements defined by numerically integrated shape functions. Special quadrature rules are suggested for triangular elements. The degeneration of these elements to crack tip elements and the direct evaluation near the singularity of element quantities, such as the stress intensity factors, is discussed.

Notizen: A finite element model for analysing static problems of three-dimensional thin panel constructions is presented. The potential energy of stretching of each panel is represented by the classical Lagrange formulation of two-dimensional elasticity and in the finite element discretization piecewise linear u, v displacements are used. The potential energy of bending of each panel is represented in the finite element discretization by Hellan and Herrmann's model, i.e. piecewise constant moments and piecewise linear w-displacements are used. The physical appropriateness of the model is verified for different assemblages of panels, and in all cases good numerical results are obtained.

Notizen: A general method to formulate improved quadratic elements is presented. Derivation of the method is based on more accurate shape functions that take into account effects of governing differential equations. These new shape functions have the same form as those of the standard eight-node quadratic element. Therefore, they may be easily adapted into existing programs.The new quadratic element is compared with both standard and standard condensed quadratic elements. To show relative merits of different quadratic elements eigenvalue tests are performed. Several examples ranging from field problems, plane stress and bar vibration are used to demonstrate the applicability of this approach.

Notizen: The exact solution to the quasi-static, inelastic response of a cantilever beam of rectangular cross-section subjected to a bending moment at the tip is obtained. The material of the beam is assumed to be linearly elastic-linearly strain-hardening. This solution is then compared with three different numerical solutions of the same problem obtained by minimizing the total potential energy using Gaussian quadratures of two different orders and a Newton-Cotes scheme for integrating the strain energy of deformation. Significant differences between the exact dissipative strain energy and its numerical counterpart are emphasized. The consequence of this on the non-linear transient responses of a beam with solid cross-section and that of a thin-walled beam on elastic supports under impulsive loads are examined.