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  • 101
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Unilaterally ovariectomized and intact cattle were injected with 1,500 i.u. gonadotropins to induce superovulation, inseminated at estrus and autopsied 30 to 60 days post ovulation. The allantochorion was dissected in situ and the viability and position of embryos in utero measured. There was no difference between the intact and unilaterally ovariectomized animals in the number of corpora lutea. One to ten embryos were recovered per cow; intact animals had more embryos than the ovariectomized ones. One to two embryos migrated per uterine horn; the percentage of transuterine migration was lower in the intact animals than in the ovariectomized ones. In unilaterally ovariectomized and intact animals, a single uterine horn could sustain only one or two embryos. When the uterine horn contained one embryo, the embryo occupied the middle or upper two-thirds of the horn. With the increase of the number of embryos the sites of embryos were unevenly spaced and overcrowded. This phenomenon led to a high rate of fetal mortality especially when the uterine horn contained more than two fetuses and transuterine migration failed to occur. The results are discussed in relation to endocrine mechanisms and species differences as they affect uterine contractions.
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  • 102
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 148 (1964), S. 253-426 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Abstracts of papers presented at the 77th meeting of the American Association of Anatomists at the University of Colorado School of Medicine, Denver, Colorado, March 31, April 1, 2, 3, 1964.The abstracts are listed in alphabetical sequence by senior author in four lists as follows: papers presented from platform, papers read by title, demonstrations and motion picture demonstration abstracts.Names of authors who are guests of the Association are marked with an asterisk.
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  • 103
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 149 (1964), S. 13-21 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A method is described for making simple balloon cannulas. These may be inserted into the depths of the brain of the experimental animal and inflated, resulting in the production of a permanent destructive lesion characterized by hemorrhagic necrosis of the parenchyma, elements of the stroma being preserved peripherally. In time, a smooth, well-defined membrane delineates the oval shaped cavity resulting in a sharply punched-out lesion. A reversible lesion cannot be produced by this technique. Such lesions may have further clinical or experimental value. The local application of radioactive isotopes to specific areas of the brain under controlled situations permitting withdrawal of solutions may be possible by this method.
    Additional Material: 6 Ill.
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  • 104
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A simple, rapid method is presented which permits qualitative evaluation of osteogenesis in tissue culture. Eleven-day embryonic chick tibiae, with distal and proximal condyles removed, were grown in organ culture for two weeks. Explants demonstrated subperiosteal osteogenesis, bridging of fracture defects, and formation of bone, de novo, over the cut cartilaginous end surfaces of explants.The method presented herein has several advantages over those previously described and may be adopted for an in vitro assay of materials to determine direct effects on osteogenesis. Large quantities of explants can be prepared easily and cultured to yield consistantly abundant quantities of osteoid. Bone which is formed over ends of anlage is particularly significant by virtue of its position. New bone formed elsewhere in explants, with the possible exception of the fracture sites, however, is difficult to distinguish from bone present before onset of cultivation. Further more, osteogenesis which occurs over the cut cartilaginous ends after a week of culture, probably results from favorable environmental conditions in vitro and not from cellular activity “preprogrammed” in ovo.In addition to osteogenesis, alteration of cartilage cells and matrix were observed in this system. Chondrocytes in the cut ends of the anlagen became spheroidal and resembled osteoblasts. Bone formation, however, was not observed about these cells. In and about lacunae of degenerating chondrocytes at the ends of the anlagen, collagen fibers appeared.
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  • 105
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Pregnant mice of Swiss ICR/Ha and the A/St and the C3H/He inbred strains were given thalidomide by oral intubation for four or five days beginning six or seven days postconception. Congenital anomalies including skeletal malformations were induced with doses of 31 to 250 mg of thalidomide per kg of body weight. Skeletal malformations of the long bones, head and trunk were seen in radiographs; soft tissue malformations were limited to structural malformations of the brain, seen in histological preparations.Because of the relatively small number of Swiss and C3H mice used, no attempt was made to discern the severity or pattern of malformations presented by each strain.
    Additional Material: 3 Tab.
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  • 106
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A study of 40 racing Greyhounds and 60 mongrel dogs provided comparative data on the respective components of the canine electrocardiogram. Additional data revealed that the Greyhound had a heart weight  -  body weight ratio of 1.25 gm, a heart rate of 115 beats per minute, a systolic blood pressure of 254 mm of Hg, and a diastolic pressure of 139 mm of Hg. These findings in an athletic animal are compared with similar observations in untrained animals. The significance of this basic information is presented and discussed.
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  • 107
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A method for rapid freezing is described in which use is made of the good heat conducting properties of silver. The freezing was accomplished by bringing the tissue in contact with a polished silver surface at the temperature of liquid nitrogen either at atmospheric or reduced pressure. Helium gas flowing over this surface prevented the condensation of water or air on the silver. After freezing the tissue was placed in a substituting solvent. The best results were obtained with 2% osmium tetroxide in acetone at -85°C. The ultrastructure of the tissue was well preserved in a narrow surface layer only.
    Additional Material: 2 Ill.
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  • 108
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    The @Anatomical Record 149 (1964), S. 405-410 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Methods are described for the isolation of two separate fractions from guinea pig thymus gland; i.e., Hassall's corpuscles and stromal segments. Hassall's corpuscles are relatively dense epithelial aggregates sedimentable at low centrifugal acceleration, while the lighter stromal segments remain in the supernatant fluid. Solubilization of the thymocytes, effected by high-speed homogenization in hypertonic glycerol, is unaccompanied by visible gelation or nuclear residue. Glycerol-sensitivity resembles radiation-sensitivity in many respects.
    Additional Material: 5 Ill.
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  • 109
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    The @Anatomical Record 149 (1964), S. 443-447 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The mesonephric duct was studied in female camel fetuses (Gärtner's duct). All fetuses were well developed, aged from two to three months. The female internal genital organs were sectioned at 10 μ and stained with hematoxylin-eosin or Van Gieson's stain.In camel fetuses, the duct is usually lined with one layer of cuboidal or columnar epithelium which may be ciliated. Degeneration and cavitation occur in the lower vaginal part, which finally obliterates the previous structure, or changes it to many layers. These two processes occur in succession at different levels of the vaginal portion and are not uniform stages along the whole duct. The appearance of the duct is variable at different levels and at the same level in different ducts. The two ducts may leave the submucosa superiorly and enter the musculature of the vagina at different levels. No glands opening into the mesonephric duct were observed. The upper (mesosalpingeal) part is smaller in caliber than the lower and seems to be immune to the obliteration and cavitation mentioned.
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  • 110
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    The @Anatomical Record 149 (1964), S. 485-489 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 111
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The four pharyngeal pouches, pharyngeal grooves, and closing membranes of the dog form in an antero-posterior sequence but the encroachment of head mesoderm which results in obliteration of the closing membranes occurs in a posteroanterior sequence. No pharyngeal clefts are formed. The anlage of the auditory tube arises as a dorso-lateral extension from the dorsal surface of the first pharyngeal pouch and expands into an elongated tube directed toward the developing ear. The second pharyngeal pouch develops into a distinct tonsillar sinus dorso-lateral to the base of the tongue. The palatine tonsil extends into the tonsillar sinus, and both become covered by a fold of epithelium. The third pharyngeal pouch extends medially toward the developing thyroid after contacting the cervical sinus. The distal tip of the third pouch differentiates into Complex III which transforms into parathyroid III and thymus III. The distal tip of the fourth pharyngeal pouch, Complex IV, also extends in a medial direction after contacting the cervical sinus. Complex IV transforms into parathyroid IV and the ultimobranchial body.
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  • 112
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Procedures for experiments on the embryo of the turtle are described. Methods of obtaining the gravid females, their handling, and preoperative preparation of the egg are described. The actual operation can be carried out much as it is done on the chick embryo. Post-operative care is described. Survival can be satisfactory. Hazards of the procedure are discussed and means of controlling them indicated. Most of the experiments were done with embryos of the common snapping turtle, Chelydra serpentina. A few experiments were done with embryos of the painted turtle, Chrysemys picta, and of the soft-shelled turtle, Trionyx spiniferus. The embryos of the painted turtle promise to be well suited to use for experimental studies. The soft-shelled turtle is not such a promising form.
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  • 113
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    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 149 (1964), S. 217-223 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: While studying the electrocardiograms of developing hearts it was noted that an adult type ECG could not be obtained in an embryonic heart which could not be blocked with digitalis. The need to know more about heart-block was obvious. Intact embryos of 36 to 120 hours were floated on to glass plates and treated with digoxin-Tyrode solution. Before 36 ± hours no dissociation of beat could be produced. The heart just stopped. At 40 ± hours a conal block followed by a midventricular block appeared. At 42 ± hours conal and midventricular blocks were followed by the first appearance of AV block. It was intermittent. At 47 ± hours conal block jumped to an incisive AV block. Midventricular block was rare. At 72 hours conal and then AV block occurred. Combinations such as four atrial to two ventricular to one conal beat could be readily produced while blood circulated.Electrocardiograms and cinephotomicrographs were taken of 72 and 96 hour hearts. Most striking was the fact that the typical Wenckebach phenomenon of adult heart-block could be duplicated, i.e., an increase of PR interval preceding AV block followed by a shorter PR interval which again gradually increased preceding AV block. This sequence was repeated over and over. An explanation of these events is offered.
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  • 114
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Embryonic thymus provides a distinct advantage over the definitive thymus because the earliest formation of lymphocyte and lymphocytic precursors can be observed in a relatively simple and uncomplicated situation. Special cytological techniques combined with light, phase, and electron microscopy have been performed in this investigation involving over 150 chick embryos between 5 and 18 days of embryonic development.Sequential cytological changes in the development of the thymus into an active lymphocytopoietic organ indicate that lymphoblasts develop by the gradual proliferation and transformation of “undifferentiated” epithelial cells comprising the primordial thymus. Lymphoblastic transformation begins on the seventh day of embryonic development in the chick and is characterized by increased cytoplasmic and nucleolar basophilia and chromatin condensation. “Undifferentiated” epithelial cells undergo two distinct lines of differentiation between the seventh and tenth days: into lymphoblasts and into stellate reticular-epithelial cells which constitute the organ parenchyma. All stages of lymphocytic maturation may be observed by 10-11 days as the thymus assumes a predominately lymphocytic character. Absence of lymphocytes or lymphocytic precursors in the connective tissue surrounding the embryonic thymus before and during the period of initial lymphoblastic transformation; presence of a continuous basement membrane surrounding the developing thymus; and absence of cells passing through this membrane during this phase of development indicate that the lymphocytic elements appearing in the embryonic thymus parenchyma are of epithelial rather than mesenchymal derivation. Although a contribution of mesenchymal elements to the lymphocytic population via vascular invasion and lobular formation in later stages of thymic development is unlikely, this question cannot be answered at this time. In spite of functional immunological distinctions between the lymphocytes of the chick thymus and bursa of Fabricius, the similarity of origin of the lymphocytic elements of these lympho-epithelial organs is apparent.
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  • 115
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cytoplasm of the megakaryocyte in the rat spleen possesses three zones, the perinuclear, intermediate and marginal. The perinuclear zone is characterized by the presence of Golgi apparatus, ribosomes, endoplasmic reticulum, and mitochondria. These organelles are found also in the more voluminous intermediate zone which in addition exhibits platelet granules and an extensive development of vesicles from smooth-surfaced endoplasmic reticulum to form demarcation membranes by coalescence. The marginal zone is almost devoid of the organelles and inclusions present elsewhere. Shedding of platelets appears to occur by extension of a paired demarcation membrane from the intermediate zone to the cell membrane and subsequent separation of its lamellae so that all of the essential organelles and inclusions of the intermediate zone may be included within the platelet. In addition, platelets contain vesicles which are probably pinocytotic in nature. Platelets are sometimes engulfed by the cytoplasm of phagocytic cells.
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  • 116
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    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 149 (1964), S. 279-297 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The tensor tympani muscle of the cat has been studied using histological and electrophysiological techniques. Histological studies revealed the presence of striated and smooth muscle fibers. The striated muscle fibers could be classified structurally as “Fibrillenstruktur” and “Felderstruktur” fibers, suggesting that both “fast” phasic and “slow” tonic fibers were present. Histochemical studies showed that some of the smaller (25-40 μ) Fibrillenstruktur fibers possessed relatively large end-plate receptor areas which stained heavily for acetylcholinesterase. The membranes and cytoplasm of even smaller diameter fibers (9-25 μ) stained for acetylcholinesterase, similar to its distribution in muscle spindles of other muscles.Intracellular recordings showed that there were two distribution peaks of the resting membrane potentials  -  one at 40-50 mV, the other at 70-80 mV. When the nerve to the tensor tympani was stimulated by single square wave pulses, small junctional potentials (40 mV) followed by an after-hyperpolarization, were recorded only from fibers with low resting membrane potentials  -  presumably slow Felderstruktur fibers. Large (70-90 mV) potentials which showed overshoot of zero potential, and which were preceded by initial long depolrizing potentials, were recorded from fibers with large resting membrane potentials. These fibers, which showed occasional spontaneous activity were presumably smooth muscle fibers. Potentials similar to those recorded from fast muscle fibers in other muscles were also occasionally recorded.It was concluded that the cat tensor tympani possessed slow and fast striated muscle fibers, smooth muscle fibers, and possible some embryonic type muscle fibers.
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  • 117
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    The @Anatomical Record 149 (1964), S. 313-318 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The hymen at different stages of life from birth to menopause was examined under the microscope. Each section was stained with hematoxylin and eosin, Van Gieson, Best's carmine, Orcein, PAS, Mallory's and modified Wilder technique. The modified Bodian's technique was used to stain the nerve fibers and the nerve cells.The main bulk of the hymen is formed of fibrous connective tissue, partly elastic and partly collagenous fibers. Both surfaces are covered by stratified squamous epithelium which lacks any evidence of cornification. The epithelium is thicker at the attached edge. Glycogen granules are equally present on the vaginal and on the vulvar surface.The connective tissue papillae are more numerous and more branching at the free edge and on the vaginal surface. There is no trace of glandular or muscle element.The hymen is not richly supplied with nerve fibers. No nerve cells and fibers are present at the free edge of the hymen. Nerve cells are bipolar, small in size, oval, spindle or kidney shaped. The terminal nerve fibers penetrate the epithelium in between the connective tissue papillae.In the newborn the hymen is vascular and the epithelium is thick. During pregnancy the epithelium is very thick and very rich in glycogen. At menopause the epithelium is thin and certain areas are cornified.
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  • 118
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A technique was devised for obtaining marrow-free trabecular bone so that the trabecular and cortical bone composition of dog, steer, monkey and man could be studied. Vertebral body was cut in the frontal plane into slices 2 mm thick with an electric band saw. The cortical bone encrircling each slice was trimmed off with a cleaver, leaving intact a lacy slice of trabecular bone. The marrow substance was washed away by fine and powerful jets of tap water obtained from an adjustable nozzle. Marrow washing was discontinued when no trace of red color was visible on holding the slice in front of a strong light source.The quantities of water, volatile inorganic, organic and ash fractions in cortical bone was alike for all species. Likewise, the trabecular bone fractions of each was quantitatively similar. In general, density and amount of ash in cortical bone was higher than that in trabecular bone. The water and ash:organic ratio were higher in trabecular than in cortical bones.Ratios of trabecular to cortical ash for the bones in an entire dog skeleton were also determined. Based on this, volume or mass of the various bone fraction may be estimated when the weight of the ash for any particular bone is known.
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  • 119
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    The @Anatomical Record 149 (1964), S. 333-357 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Comparison of results obtained by S35O4= autoradiography with those from histochemical staining methods and evaluation of the effect on the staining resulting from prior sialidase digestion, methylation, methylation and saponification, or sulfation, substantiates the selectivity of some procedures for sialomucin and others for sulfomucin. These two types of mucopolysaccharide may be differentiated by the high iron diamine-alcian blue or aldehyde fuchsin-alcian blue sequences or comparison between staining with the alcian blue pH 2.5-PAS sequence and staining with the alcian blue pH 1.0-PAS method. These techniques reveal that some rodent or lagomorph salivary glands form sialomucin alone, others apparently only sulfomucin, whereas a number produce both types of acid mucopolysaccharide either in different mucous cells throughout the gland or in the same cell.Differences in affinity for basic dyes, periodate reactivity, and susceptibility to elimination of basophilia by methylation or enzymatic digestion are evident among both the sulfo- and the sialomucins in different glands. After brief saponification the sialomucin in rat sublingual glands becomes sialidase digestible and gains alcohol resistant metachromasia.Several serous or seromucous glands secrete neutral mucopolysaccharides; others secrete polysaccharides containing sulfate or sialic acid groups whose affinity is masked for some but not for other basic dyes.
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  • 120
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Proliferative activity of the adrenal cortex was studied following injections of thymidine-H3. Normal male Long-Evans rats had less activity than comparable animals of the Sprague-Dawley strain. Of the Long-Evans rats killed at different hours of the day, those injected with thymidine-H3 at 6 P.M. and killed six hours later had the greatest activity. Normal male rats 35 to 73 days of age had almost all activity in the glomerulosa and outer fasciculata of the cortex. This activity was greatly increased two or three days after injecting colchicine, compound 48/80, or carbon tetrachloride. Such stressing agents did not cause any increase in activity during the first 24 hours. Three days after carbon tetrachloride a considerable increase in proliferative activity occurred in the inner zona fasciculata and reticularis of the adrenal of female rats during diestrus. Male rats killed 28 to 40 days after injecting thymidine-H3 showed no evidence of centripetal migration of cells into the reticularis during that time. The absence of centripetal migration and the marked responsiveness of the inner fasciculata and reticularis of female animals after stressing agents supports the zonal theory of function in the adrenal cortex.
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  • 121
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A simple method for separating the ectodermal (chorionic) and entodermal (allantoic) layers of the chorio-allantoic membrane has been devised. It consists of placing, unfolded, a membrane fragment on a rubber balloon filled with water; air is blown briskly upon the membrane by means of a rubber bulb until its shiny surface becomes dull; following this, a glass slide is applied on it, surface to surface, and then it is suddenly taken off, whereby the desired epithelial layer becomes attached to the glass surface. The microscopic observation of the layers thus individualized shows, on frontal view, their histological architecture, and the characteristic structural details of the constituent cells (cytoplasm, nucleus, nucleolus, cytoplasmic inclusions, mitochondria, stages in mitotic cell division, etc.).
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  • 122
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    The @Anatomical Record 149 (1964), S. 449-451 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Measurements of the lengths of the shafts of the third, fourth and fifth metatarsals have been made on serial standarized radiographs of white Australian children. A statistical analysis showed that, in the boys, there were no real differences between the rates of elongation of the shafts during periods when the corresponding epiphyses became radio-opaque and those that occurred during earlier or later periods. There was a statistically significant tendency in the girls for rate of elongation of metatarsals to be more rapid before radio-opaque changes in epiphyses were noted than after.
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  • 123
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    The @Anatomical Record 149 (1964), S. 458-458 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 124
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    The @Anatomical Record 149 (1964), S. 493-558 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 125
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 126
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The anatomical and histological picture following intravenous injections of several concentrations of aqueous solutions of Alcian Blue has been described. Alcian Blue granules can be found in all cases within the reticulo-endothelial cells of the body in lungs, spleen, liver and bone marrow and in the kidney.The greatest concentration of the dye is found within the renal glomeruli. It is in such concentration as to enable one to see individual glomeruli with the 10 × magnification of the dissecting microscope. On high power (950 ×) the dye can be seen as granules within the endothelial cells and podocytes of the glomerulus, as well as in the cells of the glomerular stalk. No Alcian Blue has been identified within the macula densa.Certain difficulties due to the solubility of the dye in sections are mentioned. Present means of meeting them are covered. Also discussed are follow-up studies being undertaken and possible implications of the procedure.
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  • 127
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    The @Anatomical Record 149 (1964), S. 691-697 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Aggregation of acid mucopolysaccharides in normal palates and cleft palates in mouse embryos was studied by various histochemical methods. An “index of staining intensity” was introduced as a mean numerical value derived from the visual grading of all sections of each litter. Animals were killed at 20 different points of gestation period, from thirteenth to seventeenth day. In this manner a composite staining intensity  -  time table could be obtained for both experimental groups. The observed data seem to indicate some correlation between the concentration of acid mucopolysaccharides in palatine shelves and the competence of embryonic mechanism of palate closure.
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  • 128
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    The @Anatomical Record 149 (1964), S. 699-705 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In the female genital system of the rat the only organ to give a strong histochemical reaction for β-galactosidase with the post-coupling technique is the ovary, where strong staining occurs in the corpora lutea, the interstitial cells and some of the macrophages. In the uterus and vagina the relatively weak reaction is restricted to the epithelia, and is reduced by ovariectomy. During pregnancy the decidua shows marked activity, but in the portions of the uterus which are not involved in placentation the reaction is still restricted to the epithelium. The mucified vaginal epithelium of late pregnancy is moderately reactive. In the rabbit ovary the activity is strong, with intense staining in interstitial cells; in the ovaries of the guinea pig and monkey it is much weaker.Though β-galactosidase is considered to be mainly lysosomal, its ovarian distribution differs in some respects from the distribution of acid phosphatase. The relative intensities of the ovarian and uterine reactions in the rat, as shown histochemically, do not appear to correspond with biochemical findings.
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  • 129
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Damage produced by the multiple insertion of a needle into a mouse kidney results, in two days, in the stimulation of mitotic activity around the damaged areas, and in the cortex of the contralateral kidney. The mitotic activity in the contralateral kidney cortex approximates that seen after unilateral nephrectomy.Evidence is presented which suggests that the loss of kidney mass associated with the damage is not the cause of the increased mitotic activity in the damaged or contralateral kidneys. The release of stimulatingsubstances from the wound is probably required. This is in contrast to growth produced by unilateral nephrectomy where the loss of kidney mass is the initiating factor.
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  • 130
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    The @Anatomical Record 150 (1964), S. 35-50 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Detailed histological and histochemical studies were made on the effect of transection on the perineural epithelium of the peripheral nerves of rat and cat. Histologically and histochemically it has been shown that the perineural epithelium does not undergo any change in the proximal as well as in distal segments of these transected nerves and is maintained as seen in the untransected nerves. These studies also show that the capsule of the muscle spindle is made up of a continuation of the perineural epithelium and maintains its histological structure, as well as its enzyme equipment. Electronmicroscopic evidences are given for the normal maintenance of this epithelium in the regenerating nerve. The only structure which does not show any change when a nerve is transected is the perineural epithelium. It has been pointed out that the perineural epithelial tube plays an important role in conducting the regenerating axons within the neurilemmal tube to the proper sensory and motor endings.
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  • 131
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Adult virgin females were made pseudopregnant by cervical stimulation and four days after stimulation the uteri were traumatized with a thread. The animals were autopsied 24, 48, 72, 96, and 120 hours after trauma. Frozen sections of the deciduomata were sectioned on the cryostat. For the identification of phosphorylase the sections were placed in the substrate containing 50 mg glucose-1-phosphate (G-1-P), 10 mg muscle adenylic acid, 15 ml distilled water, 10 ml 0.2 M acetate buffer (pH 5.8), 25 units of insulin and 5 mg of glycogen. To demonstrate uridinediphosphoglucose (UDPG)-glycogen transferase sections were placed in the substrate containing 50 mg UDPG, 10 mg glycogen, 20 mg Versene, 10 mg G-6-P, 14 ml distilled water, 10 ml 0.2 M tris buffer (pH 7.4) and 1 ml absolute alcohol. Following incubation for one hour at 37° the sections were treated with either Lugol's or Gram's solution until the color developed. A positive response for phosphorylase was a blue-black color while a red-purple color indicated UDPG-glycogen transferase. The periodic-leucofuchsin technique was used to identify glycogen and hemotoxylin and eosin sections were studied for general morphology.Glycogen and phosphorylase were observed in the decidual cells. However, the enzymatic activity was not as intense or as extensive as the polysaccharide. UDPG-glycogen transferase activity was observed in only two of the 50 deciduomata studied. On the basis of the present observations it may be suggested that the important pathway for glycogen synthesis in the decidual cells is from G-1-P by the action of phosphorylase and not from UDPG by the action of UDPG-glycogen transferase.
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  • 132
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The purpose of this study was to determine the dimensions of capilaries in various tissues, in vivo, in frogs, mice, and pups. Capillaries were measured in the web, strap muscles of the neck, mesentery and bladder of frogs, and in the mesentery of mice and pups. The light source was a fused quartz rod transilumination apparatus. In all cases the exposed tissues were kept moist by a continuous flow of Ringer's solution over their surfaces.The mean values found for the tissues of the frog are web 11.94 μ, skeletal muscle 11.30 μ, mesentery 11.97 μ, and bladder 11.40 μ. The mesenteric capillaries of the mice and pups had a mean value of 4.64 μand 7.36 μ respectively.Difficulties encountered during the course of observation included vibrations of the optical equipment, movement of the tissue under the microscope, fogging of the objective lens, and the determining of which of the microscopic vessels were “capillaries.”A statistical analysis of the data indicates that the variance in the measurements recorded on the capillaries of a single animal is greater than the variance from animal to animal.
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  • 133
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    The @Anatomical Record 150 (1964), S. 215-219 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Physical anthropological and x-ray linear measurements of length, breadth and height of 28 dried skulls were made. The means and variances of these measurements were calculated and are given in tables. Next, the cranial capacity of these skulls was measured with mustard seeds (this method was validated using water measured skull) and the means and variances are given separately for males and females. Two formulae: (1) Lee's - Males, 0.365 (L × B × H) + 359.34; Females, 0.375 (L × B × H) + 296.40. (2) Spheroid-π/6 (L × B × H) were applied to calculate cranial capacity (in milliliters) from the linear anthropological and radiological dimensions (in centimeters). The results of the volumes thus obtained were compared to each other and to the control volume of the respective skulls. It was concluded that both the Lee's and Spheroid formulas for calculating the volume showed acceptably small difference from the respective control values. However, the Lee's method gave closer to control volumes for anthropological measurements whereas the Spheroid method gave closer to control volumes for x-ray measurements.
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  • 134
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Macroscopic and neurohistological observations of the tongues of the pigmy and common marmosets are described. On the dorsum, there are three circumvallate papillae arranged in an inverted V-shape. Through the magnifying glass the foliate papillae with a few clefts are found anterior to the attachment of the glossopalatine fold. The fungiform papillae can be seen with a lens. The vallate papillae are supplied with abundant nerves. The taste buds are located only in the lateral non-cornified wall of the papilla, closely associated with the subgemmal nerve plexus. The lingual glands of the serous type are present deep in the muscle of this region. The ganglion cells composing the ganglion are located inside and below the papilla. The foliate papillae consist of a few furrows with many taste buds and are supplied with abundant nerves and a few ganglion cells. The serous glands are not plentiful. Infiltration of the lymphoid cells in the foliate papilla region should be regarded as the lingual follicle of the primitive type. The fungiform papillae are supplied with abundant nerve fibers which terminate free and are furnished with many taste buds having an embryonic appearance. The papillae are comparable to those of the human fetal or newborn tongue. The “sublingua” is a rudimentary structure having neither sensory nerves nor taste buds. The ganglion cells lying on the nerve fiber bundles in the apical region probably are cells migrating from the submandibular ganglion. No apical glands of Nuhn are present.
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  • 135
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    The @Anatomical Record 148 (1964), S. 503-505 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: UDPG-glycogen synthetase was studied in the uterus of ovariectomized and ovariectomized-hormone treated rabbits and in the tongue. Following the biochemical technic of Leloir and Goldenberg it was not possible to demonstrate the enzyme in the uterus of the ovariectomized or ovariectomized treated animals. However, in the tongue the enzyme was present. The data from this study along with previous histochemical studies indicates that the glycogen in the uterine muscle is not synthesized through the UPDG pathway, but is synthesized from G-1-P by the action of phosphorylase. Since UDPG-glycogen synthetase can be demonstrated in the tongue a difference seems to exist in glycogen synthesis between the smooth muscle of the uterus and skeletal muscle of the tongue.
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  • 136
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    The @Anatomical Record 149 (1964), S. 1-11 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ectopic glomeruli and proximal convoluted tubules were found in the kidneys of the rat, cat, dog, rabbit and ferret and in the human full-term fetus. They lay in the connective tissue around the main intrarenal vessels and deep to the pelvic mucosa. Their afferent arterioles were long and gave branches to the pelvic mucosal plexus, while their efferents supplied the pelvic mucosa and the medulla, or one or the other of these tissues. In the rabbit the ectopic glomeruli often produced small cysts in the pelvic septum. Degenerating ectopic glomeruli were occasionally found and in the dog the majority of them showed this change. In the adult human kidney, aglomerular arteries supplied both the pelvic mucosa and the medulla and it is suggested that such arteries are the result of degeneration of ectopic glomeruli.
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  • 137
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The adrenal cortex of the male guinea pig has been examined with the electron microscope. Many of the cells of the zone glomerulosa possessed bizarreshaped nuclei with frequent cytoplasmic invaginations. The internal structure of the mitochondria was entirely cristaform, the cristae being spaced at reasonably regular intervals. A rough-surfaced endoplasmic reticulum was present in this zone only. The outer fasciculata showed an abundance of lipid in droplet from. The mitochondrial internal structure of this zone was predominantly cristaform, but the cristae were aggregated in clumps instead of being evenly spaced. Smooth-surfaced cytoplasmic vacuoles were frequent. The inner fasciculate and the reticularis were generally similar. They differed from the above zones in that little lipid was present in droplet form and by the presence of a profusion of smooth-surfaced cytoplasmic vacuoles which were interpreted as being homologous with the agranular endoplasmic reticulum. Mitochondria of the inner fasciculata were irregular in shape, while those of the reticularis were larger and more regular. In all zones of the cortex, the vascular channels were lined with an endothelium separated from the parenchymal cell by a space which was frequently seen to contain fibrils as well as fibroblast-like cells.
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  • 138
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    The @Anatomical Record 149 (1964) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 139
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The medullae from the right adrenal glands of hamsters given one or three injections of reserpine were prepared for electron microscopy by fixation in buffered osmic acid while the contralateral left glands were fixed in formol-dichromate for light microscopic determinations of the chromaffin reaction. The adreno-medullary cells of these animals were compared with cells of untreated hamsters in order to determine if catecholamine loss was accompanied by changes in the electron opaque cytoplasmic granules.Ten and 15 hours following one reserpine injection (1 mg/kg) the chromaffin reaction was positive, whereas after 20 hours the reaction was negative, reflecting a catecholamine reduction. The negative chromaffin reaction was accompanied by alterations in granule ultrastructure. The dense central core of the granules was less electron opaque compared with those of control medullary cells. The decreased density likely reflected a catecholamine loss since the opaque appearance of the granules is attributed to the presence of the amines. Following three injections (1 mg/kg daily; three days) the chromaffin reaction was negative and most granules had disappeared from the cytoplasm. The results give evidence that, after limited reserpine injections (1 mg/kg; one injection), catecholamines can be released from the medullary cells without granule disappearance.
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  • 140
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    The @Anatomical Record 149 (1964), S. 299-307 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A cyanotic child and a newborn puppy were found to have a complicated cardiac malformation of essentially identical design. There was situs inversus of the cardiac chambers, hypoplasia of the anatomic left heart with transposition of the great vessels, pulmonary atresia, patent ductus arteriosus and drainage of all pulmonary veins into the anatomic right atrium. There was situs inversus totalis with pivotal levocardia in the child and dextrocardia in the puppy.The patient was markedly improved by a systemic-pulmonary arterial anastomosis indicating the feasibility of palliative surgery in spite of the servere cardiac abnormality.The association of visceral heterotaxy with an identical complicated malformation of the heart in the human and canine species appears to indicate a common developmental deviation and thus is considered to be of teratologic significance.
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  • 141
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    The @Anatomical Record 149 (1964), S. 67-72 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This study points out a heretofore unrecognized range of variation in the mastoid area, between modern and ancient man. The mastoid region of modern man was re-investigated and found to present a very different picture from fossil man. A trend was noted in modern man toward the enlargement of the mastoid process, the eruption of the juxtamastoid process in the digastric fossa and the absence of a pronounced occipitomastoid crest. The conditios were considerably differnt in Neanderthal man. In this group the area consisted of a small mastoid process, a spacious digastric fossa with no intervening juxtamastoid process and an occipitomastoid crest which exceeded or at least equaled, the projection of its mastoid process.This additional data emphasizes the cranial differences between modern man and Neanderthal man and suggests that the region should be re-evaluated in light of this new data.
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  • 142
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Vital staining by trypan blue was used to demonstrate macrophages within involuting (post-secretory), lactating and resting mammae of mice. Involution was produced by removal of the nursing young on the eighth day post-partum when maximal lactation was present. The number of macrophages in involutin mammae was not significantly greater than that found in the rsting glands of mice receiving comparable injections of dye. Macrophages (coarse, granular accmulations of trypan blue in cytoplasm) in involutin mammae were congregated around and within atrophic alveoli and small ducts, while in lactating and resting mammae they were located primarily in the areolar stroma. Plasma cells, lymphocytes and neutrophils were increased during involution, but the limited leucocytic response did not indicate that a significant inflammatory reaction was active in the process of involution. Difuse parenchymal staining, indicating cellular injury or death, was limited to the epithelium of necroptic ducts and alveoli of involuting glands. Granular deposits of dye, as in macrophages or renal tubular epitelium, were not seen in the resting, lacating or involuting mammary parenchyma.
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  • 143
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The right knees of rabbits were rigidly fixed with metal clamps midway between full flexion and full extension. After six dayas the knees of six were preserved while clamped. Twenty other rabbits were sacrificed after 16 days. Ten received colchicine on the eighth, eleventh and fifteenth day following clamp application. Their knees were preserved after clamp removal. The femoral and tibial articular cartilages subjected to pressure for six days were extremely compressed but viable. In knees clamped for 16 days the changes found were related to the amount of knee movement present at autopsy. Where it was small but easily detectable the cartilages were normal. Where there was no movement the cartilage was dead. Where it was only slight variable cartilage changes were found ranging from surface abradement, Matrix swelling, enlargement of lacunae and chondrocytes, increased number of cells per lacuna, and an occasional chondrocyte in mitosis, to an extensive matrix alteration resulting in the disappearance of lacunae, chondrocytes released from the confines of lacunae and interspersed among a meshwork of fine collagen fibers, and a few released chondrocytes in mitosis. The most extensive change was where the cartilage defect area was occupied by a mass of proliferating richly-cellular tissue. The knee articular cartilage changes of this experiment are compared with those occurring during pregnancy in the pubic symphyseal cartilage of mice.
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  • 144
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: It is well known that cadmium chloride administered as single, subcutaneous, sub-toxic doses selectively damages the rat testis, producing irreversible injury of the germinal epithelium and temporary damage to the interstitial tissue.Studies reported here demonstrate that: (1) this injury represents an ischemic necrosis secondary to rapid production of intertubular edema (4 to 6 hours), increased intratesticular pressure, with or without associated hemorrhage, and ultimate interference with testicular blood supply and drainage - not unlike injury following testicular artery ligation; (2) as dosage is progressively reduced to approximately one-sixth that employed by most investigators, interstitial tissue is unaffected and ischemic necrosis is supplanted by a non-necrotic degeneration of the germinal epithelium closely resembling, in its histopathology and irreparability, that occurring after vitamin E deficiency; (3) oral administration of vitamin E has no protective effect, and testes of rats critically depleted of E show no increased susceptiblity to cadmium.Observations on the nature and distribution of tubular injury, and on the vasculature of the testis and epididymis, suggest that the unusual sensitivity of the testis to cadmium is related to unique features of its vasculature; namely, the pulseless, semistagnant flow of blood in the intratesticular course of the testicular artery, which permits cadmium to alter capillary endothelium permeability, causing edema and pressure effects leading to acute anoxia at higher levels of dosage, and diminishing degrees of anoxia with decreasing dosage levels. Comparable morphological features of the proximal segment of the caput epididymis make it likewise susceptible to cadmium injury.
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  • 145
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A study of nerve-muscle relations and fine innervation patterns is reported for the stapedius muscle of the cat. Gross relations of the muscle to its associated nerves and other middle ear structures are shown by illustrations. Histologic features of innervation are studied with the Bodian silver method. the thiocholine technique, and iron hematoxylin and osmic acid stains.The muscle is richly supplied by 3 to 4 facial nerve branches in which no axons are found above 8 μ and the majority are unimodally grouped at 2-4 μ. The auricular nerve of the vagus does not penetrate the muscle. Three to four distinct zone is of fasciculi are found whose muscle fibers are 14-20 μ in diameter. Each zone is supplied by primary nerves whose branches do not exhibit terminal overlap or multiple innervation of muscle fibers. Short terminal axons lead to motor and plates from all intramuscular nerve branches. Nerve branches and motor end plates are confined to the basal third of the muscle. Sensory endings are not detected within the muscle or its tendon. It is estimated that each motor neuron supplies no more than five muscle fibers but more probably three or less.
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  • 146
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Large intracellular ice formations are assumed to be more damaging than small ones. Nuclear alteration, such as pycnosis, generally is considered a reflection of reduced vital cellular functions. Evidence is presented which shows, on the basis of rate of oxygen consumption, that survival of kidney cells is greater after the formation of large rather than small intracellular ice artifacts, and that nucelar alteration such as pycnosis does not indicate the degree of damage to a vital function, their respiratory activity. Findings agree with those based upon autotransplantation of skin (Anat. Rec., 144: 171-191, '62). It is suggested that mitochondria are one of the cellular sites of freeze-thaw injury and that the mechanism of freezing injury may differ in nucleus and ctyoplasm.Microscopic appearance of ice artifacts formed in cells of mouse kidney slices after slow (1.5° and 3.0°C/min) and rapid (19° and 38°C/sec) freezing to -75° and -196°C was preserved by the author's freeze-drying method. Control (unfrozen) and frozen-thawed slices were fixed in Bouin's fluid and processed routinely for study of microstructure. Oxygen consumption of parallel groups of control and frozen-thawed slices was measured by the conventional Warburg manometric technique. The data revealed that: (1) the size of intracellular ice artifacts was 5 to 17 times larger in slices frozen at slow than at rapid rate; (2) Q02 of cells in slowly frozen tissue slices (average of 21% of control Q02) was significantly greater than rapidly frozen cells (average of 9% of control Q02); (3) Nuclear pycnosis, crenation and vacuolation were more extensive in cells which were frozen slowly.
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  • 147
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    The @Anatomical Record 149 (1964), S. 623-649 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Human embryonic myocardial tissues ranging from eight to eighteen weeks of development were prefixed and dissected in buffered formaldehyde, postfixed in buffered OsO4 and processes for Epon embedding. At these stages of development the myocardial cells contain an abundance of sarcoplasm with a sparse random distribution of myofibrils that frequently appear branched. The A, I and Z bands exhibited in the adult myocardial cells are also observed in these stages of development. However, the M line is only slightly apparent, while no H band was observed. The mitochondria are not arranged in parallel arrays as found in the adult, but appear to be randomly distributed throughout the interfibrillar sarcoplasm. An array of tubules, cisternae and vesicles that comprise the sarcoplasmic reticulum is also distributed throughout the interfibrillar sarcoplasm. In some areas this tubular system appears as vesicles with small dense granules along its outer surface, while in other areas it appears smooth. The distribution and orientation of the sarcoplasmic reticulum is far less pronounced in the embryonic stages examined in this study than that reported for the adult myocardium. Dense granules (150 to 350 Å) appear as clusters or aggregates of granules in the sarcoplasm and are similar to the glycogen granules described by other investigators. The intercalated disc is observed at the junction of two opposing cells and occurs with less frequency than the intercalated disc in the adult myocardium.
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  • 148
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Histological techniques were used to obtain fiber counts, capillary counts, fiber diameters, and capillary-fiber ratios in the adult rat heart. Based on measurements of 3,600 fibers in six rats, the means and standard deviations of the fiber diameters for the left and right ventricles were 11.8 ± 4.8 and 11.5 ± 4.3 μ respectively. The corresponding capillary-fiber ratios were 0.92 ± 0.23 and 0.90 ± 0.33. The differences between the right and left ventricles were not statistically significant. Statistical analysis was used to evaluate the reproducibility of the results. The frequency distribution of the fiber diameters was found to be unimodal in both right and left ventricles suggesting that only one group of fiber sizes is involved. It was found that fiber counts could be used as an indication of fiber size under ordinary conditions provided that the total number of fibers counted is over 200.
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  • 149
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    The @Anatomical Record 149 (1964), S. 411-433 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The distribution and origin of interstitial tissue has been studied in various mammalian ovaries with histochemcal techniques for lipids. The interstitial tissue contains sudanophilic droplets of different sizes consisting of phospholipids, triglycerides and cholesterol and its esters; the lipids are comparatively unsaturated.The granulosa of normal follicles contains heterogeneous lipid bodies of various sizes composed of unsaturated phospholipids. With the start of atresia these lipid bodies also develop neutral fats (triglycerides). Besides the heterogeneous lipid bodies, another type of sudanophilic lipid droplets of atresia, consisting of triglycerides, cholesterol and its ester and little phospholopids, are formed in the granulosa cells. When the number of these lipid droplets is sufficiently increased, the heterogeneous lipid bodies disappear from the atretic follicles. The theca of such atretic follicles hypertrophies and persists to form the interstitial tissue whereas the granulosa cells, along with the lipid droplets, regress and disappear.In the rat ovary, the interstitial tissue occurs as scattered patches of various sizes that are derived from the theca of atretic preantral and antral follicles. Most of the lipid droplets of the interstitial tissue are mobilized from the preovulatory rat ovary. All of the bat ovary is occupied by interstitial tissue except for the cortex; the tissue is formed in the same way as in the rat. In the cat ovary, the interstitial tissue is sparsely scattered between the follicles and originates from the theca and adjacent stromal cells of normal and atretic follicles. In the dog ovary the interstitial tissue has a similar origin and also arises from invaginations of germinal epithelium. In the opossum ovary the interstitial tissue is present in the form of sparsely scattered patches of cells which arise from the theca of large preantral follicles. A few lipid bodies, consisting of unsaturated phospholipids, appear in the interstitial tissue.
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  • 150
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: An electron microscopic study has been made of the constituents of the lamina propria of the stomach, small intestine and large intestine of the mouse. This layer is highly cellular in the intestines, less so in the stomach. The connective tissue cells are often crowded together without detectable fibers or ground substance between.Below the basement (boundary) membrane of the epithelium attenuated fibrocytes form an almost continuous layer. The appearance of a fibroblast in mitotic division is described.In the connective tissue spaces are abundant plasma cells, macrophages, undifferentiated cells and eosinophils. The fine structure of each is described and illustrated.Particularly evident is the frequency with which plasma cells lie in direct contact with macrophages and with eosinophils. In some instances, vesicles of similar appearance occur in apposed plasma cell and macrophage, suggestive of exchange of material.These close relations of macrophages, plasma cells, and eosinophils are discussed in terms of the role each plays in the defense of the organism against foreign antigens.
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  • 151
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    The @Anatomical Record 149 (1964) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 152
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: With the aid of a Zeiss operation microscope, a dissection was made of the nerves to the blood vessels of the dorsum and sides in the two feet of the cadaver of an adult man. No obvious vascular nerve issued from the saphenous nerve. The terminations of the dorsal metatarsal and interdigital arteries and veins over the two medial thirds of the dorsum of the foot were supplied by extremely thin nervelets which originated chiefly from the medial branch of the superficial peroneal nerve. The sural nerve issued conspicuous filaments to the lateral interdigital and metatarsal blood vessels, to the lateral calcaneal arteries and to the talo-calcaneal region. These findings provided a firmer anatomical basis for the interruption of the vascular nerves of the dorsum and sides of the foot by blocking with analgesic agents or by crushing the superficial peroneal, sural and saphenous nerves above the ankle.
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  • 153
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A new and relatively simple technique for conducting developmental neurology experiments is described. This technique consists of subjecting rats less than four days in age to a collimated source of external beta radiation that can be directed to selected parts of the brain. Use of this method yields the following advantages: (1) the size relationships of the parts of the brain can be altered, (2) bilateral asymmetry of the cerebrum, and/or cerebellum can be produced, (3) chronic functional defects which develop as the rat develops can be obtained, (4) the experimenter may selectively destroy any site in the brain provided that it is superficial enough, and (5) foci of epilation appear on the scalp and serve as markers. The limitations of this method are also discussed.
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  • 154
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    The @Anatomical Record 149 (1964), S. 651-669 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Host tissue underlying skin homografts and isografts was removed 4 to 8 days after grafting and fixed in osmium tetroxide for electron microscopy. The skin homograft bed was composed of a dense mass of cells, with bundles of collagenous fibers interspersed. Two types of large, elongated cells with elongated nuclei were commonly seen, one characterized by extensive granular endoplasmic reticulum and the other by masses of free ribosomes. The former corresponds to descriptions of fibroblasts and the latter resembles a lymphoid cell but is considerably larger than those seen in peripheral blood. These two cell types contain most of the ribosomes present in the graft bed. Numerous macrophages and neutrophils were present also. The skin isograft bed was similar except that no large elongated cells with masses of free ribosomes were found. The cell types identified with the electron microscope correspond to the cell types proposed earlier on the basis of light microscope radioautography with thymidine-H3. It is suggested that the elongated, hypertrophied, lymphoid cell, rather than the macrophage, is responsible for homograft rejection.
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  • 155
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: One hundred twenty-two of 225 accurately located and measured mammary segments were transplanted successfully into the isologous female mice, and intact or castrated male host mice treated with estrogen and progesterone pellets. Five of 42 segments that had ligatures placed to determine the orientation of the segment were also recovered. Fewer grafts were recovered from intact male hosts than from castrated male or intact female hosts. The lengths of transplants ranged from less than 0.2 mm to 3.0 mm. Transplants that were 0.6 mm long grew most frequently. Transplants obtained from different portions of any one mammary gland or from any mammary glands were recovered at comparable rates. The fourth mammary gland-free fat pads, the pararenal fat pads, the mesometrial fat layers and subcutaneous areas were used as transplantation sites. The ultimate size of the regenerated transplants was determined by the amount of adipose tissue in the transplantation site and the hormonal environment, but not by the original size of the transplant. Mammary transplants obtained from all portions of ducts, even from quiescent glands of a 734-day-old mouse, or grafts which had been ligated with silk or hair, showed equal capability of regeneration. All successful transplants responded to either endogenous or exogenous hormones in a manner comparable to the hosts' own mammary glands, and lactated at parturition.
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  • 156
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Variable numbers of small lymphocytes with “atypical nuclei” have been shown to occur regularly in lymph nodes from normal animals. The present studies were carried out in explanation of these cells.Mice were injected subcutaneonsly or intraperitoneally with physiologic saline or isotonic glucose, and with solutions of various organic substances in glucose. The draining nodes were fixed at various intervals thereafter, and unstained sections were examined by light and polarized microscopy.These lymphocytes increased after all of the injections. The increments were moderate and of short duration after injections of saline and glucose alone, and the nuclei were morphologically similar to those found in the controls with the techniques employed. The increments were greater and more protracted after injections of most of the organic substances, and the morphology of the nuclei in polarized light differed from that of the normal. The effect on the morphology was influenced, in part, by the degree of solubility of the organic substance and the duration of exposure of the cells to it.The conclusion is drawn that lymphocytes with “atypical nuclei” represent response to lymph drainage, in general, while modifications of their characteristic nuclear morphology are indicative of specific biologic responses.
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  • 157
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ninety-eight fetal specimens of the circulus arteriosus cerebri and adjacent arteries were injected with a mixture of latex and barium sulphate and dissected after fixation in formalin. Measurements of the intracranial part of the internal carotid artery were carried out and the measured diameters were compared to those of the other arteries at the base of the fetal brain. The diameters of the internal carotid artery were symmetrical and increased approximately linearly with fetal size from 0.7 mm for a fetus of 15.5 cm crown rump length to 2 mm in a specimen with 37.2 cm crown rump length. In one specimen, the intracranial part of the right internal carotid artery was smaller than the left. This was associated with an anomaly of the arterial circle. Twenty-one circles were symmetrical and complete. The most significant differences between adult and fetal diameters were in the posterior part of the circle, the circular part of the posterior cerebral artery showing a relatively smaller average diameter (0.34 of the diameter of the internal carotid artery) than that described for adult specimens (0.47) while the posterior communicating arteries were of similar diameter (approximately 0.27) in both adults and fetuses. In three cases one posterior communicating artery was absent. No berry aneurysms were found upon inspection with a hand lens.
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  • 158
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Sections for electron and light microscopy were easily prepared from mixtures of Dow Epoxy Resins 332 and 732 (D.E.R. 332 and D.E.R. 732) cured with dodecenyl succinic anhydride (DDSA) and DMP-30. The most useful formula contained 46.7% D.E.R. 332, 20% D.E.R. 732, and 33.3% DDSA, plus 2% DMP-30. Stronger sections were derived from a preparation containing 31.6% D.E.R. 332, 15.8% D.E.R. 732, and 52.6% DDSA, plus 2% DMP-30. Harder resins were made by reducing the amount of D.E.R. 732 incorporated in the mixtures. When prolonged infiltration was required polymerization was slowed by substituting DMP-10 in place of the more reactive DMP-30. Compressed sections were easily restored to their proper size by the vapors of chloroform, dichloroethylene or xylene. The sections were strong enough to withstand intense electron beams on bare 150 mesh copper grids. The inherent contrast was good without staining, but additional contrast was obtained by brief treatment with aqueous solutions of lead or uranyl salts. Sections for light microscopy could be stained by water soluble basic dyes.
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  • 159
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: An infrahyoid, parapharyngeal method of hypophysectomy in the rabbit has been developed. This method is easily learned and the operation can be performed in less than an hour. Complete removal is verified by examination of the sellar contents either with a dissecting microscope or histologically by fixation, embedding and serial sectioning. The operative mortality is about 10%. No hormonal supplements are required postoperatively.
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  • 160
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Suspensions of thymic lymphocytes, 60-80% of which had been labeled in vivo with H3-thymidine, were injected intravenously into 54 homologous rats and 22 isologous mice. Radioautographs2 of blood and of tissues were analyzed. The cells rapidly disappeared from the blood, although less rapidly in the isologous than in the homologous systems. A large fraction was still present in the tissues during the first post-injection day. In the first hour many were arrested in the lung, some of which were later redistributed to other organs. At later intervals the spleen contained the greatest concentration, followed by lymph nodes and bone marrow. Cells were found occasionally in the duodenal mucosa, in mice only, and in no case did they reach the thymus. A few survived in both rats and mice for as long as seven days.Only one labeled cell was seen in mitosis. There was evidence of transformation to erythroblasts in the liver, spleen and bone marrow; to myelocytes in the bone marrow; and to macrophages in the spleen. Label from some of the cells dying in the liver apparently was reutilized by the Kupffer cells.
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  • 161
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    The @Anatomical Record 150 (1964), S. 249-256 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The extent and arrangement of cardiac muscle in the great veins of adult dogs of both sexes was investigated. Cardiac muscle was found for varying distances in the venae cavae and pulmonary and azygos veins in all animals. The average distances which the muscle extended into these vessels in the beagles were: 1.88, 0.859, 0.525, and 0.622 cm. Similar measurements in the poodle were: 1.35, 0.705, 0.764, and 0.690 cm and in the shepherd: 3.05, 1.36, 1.22, and 0.926 cm. Both the venae cavae and pulmonary veins contained circular, spiral and longitudinal muscle bundles. The cardiac muscle was continuous from the superior venae cavae into the wall of the azygos vein and appeared to occupy the medial layer of the wall in all instances. Elastic and white fibrous connective tissue extended between the muscle bundles and appeared to invest the tapered muscle fibers in some places.
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  • 162
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    The @Anatomical Record 150 (1964), S. 265-269 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: By means of a new staining procedure (using a mixture of Astra blue and safranin O), studies on the polysaccharides of the mast cell granules were made in adult and embryonic connective tissues of the rat. According to their staining reactivity toward these dyes, it was found that, in the adult tissues, mast cells can be classified into three groups: (1) Astra blue positive mast cells, or “blue” cells; (2) safranin positive mast cells, or “red” cells, which are the most numerous cells; (3) intermediate forms, which exhibit affinity for both dyes, or “mixed” cells. In the embryo, mast cells appear first on the fifteenth-sixteenth day of development as small round cells, the cytoplasm of which contains flakes of Astra blue positive material; rapidly, this material becomes granular, and on the twenty-first day (just before birth), a large mast cell population exists in the subcutaneous connective tissue of the embryo, containing only cells of the “blue” type. Eight to 15 days after birth, “red” and “mixed” cells are visible. Progressively, the mast cell population becomes of the adult type. It is thought that these variations in the staining reactivity correspond to the evolution of the cell: the “blue” forms are the younger cells and the “red” forms are the adult mast cells. This is probably related to a progressive increase of the number of strong acidic groups which are present in the polysaccharidic molecule.
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  • 163
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    The @Anatomical Record 150 (1964) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 164
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The common bile duct epithelium has been studied in common bile duct-ligated and sham-operated rats treated with colchicine, and numerous colchicinemitoses have been observed largely localized in the biliary or tubular “glands.” This finding suggests that the “glands” serve as the centers of cell division, i.e., the “renewal areas,” within the duct epithelium. Evidence has also been found that the duct epithelial cells are shed from the surface (luminal) epithelium into the lumen.In these cytological features, the common bile duct epithelium resembles the small intestinal epithelium, and this suggests the possibility that the pattern of cell renewal and replacement in the common bile duct epithelium is similar to that in the small intestinal epithelium, i.e., the “gland” cells constantly divide and replace the cells which are lost from the surface epithelium.
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  • 165
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    The @Anatomical Record 150 (1964), S. 271-277 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: S35-labeled inorganic sulfate was employed as a precursor of sulfated mucopolysaccharides in a radioassay of embryonic palatine shelves of the offspring of normal and cortisone-treated mice. Samples, composed of four palatine shelves derived from the same litter, were counted on a liquid scintillation spectrometer. A close correlation has been established between the time of the maximum aggregation of sulfated mucopolysaccharides, as expressed by the relative specific activity of retained S35-labeled sulfate, and the time of “horizontalization” of palatine shelves in both experimental groups.The competence of embryological mechanism of palate closure has been related to the developmentally normal relative increase of the concentration of sulfated mucopolysaccharides, viewed as a factor maintaining maximum visco-elasticity and minimal swelling of palatine shelves.
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  • 166
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Mucosal reduplications of the ampullary mucosa in the major duodenal papilla have been found to be of two types: (1) columnar-shaped projections apparently related to the wall of the terminal bile duct in adults only, are structures acquired postnatally; (2) leaf-like flaps that are generally transversely oriented along the caudal wall of the ampullary space both in adults and in fetuses are congenital structures concerned in guarding the orifice of the main pancreatic duct against the incursion of back-flowing fluids.
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  • 167
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    The @Anatomical Record 150 (1964), S. 319-334 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The epaxial musculature of the golden hamster based on 180 specimens has been described as follows. The Longissimus dorsi Group: L.d. pars cervicis, L.d. pars thoracis, L.d. pars lumborum, Extensor caudae lateralis. Muscles Primarily Cervical in Location: Splenius, Complexus, Biventer cervicis Semispinalis cervicis, Rectus capitis dorsalis major, Rectus capitis dorsalis minor, Rectus capitis lateralis, Obliquus capitis inferior, Obliquus capitis superior, Longus atlantis. Muscles Primarily Thoracic in Location: Spinalis dorsi, Iliocostalis. Short Deep Epaxial Bundles: Multifidus spinae, Extensor caudae medialis, Intertransversarii, Interspinales.
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  • 168
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    The @Anatomical Record 150 (1964) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
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  • 169
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    The @Anatomical Record 150 (1964), S. 365-381 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Marginal bands from erythrocytes of several non-mammalian vertebrates were studied by light and electron microscopy. Cells were observed in thin sections after various fixing procedures and in wholemount preparations. Isolated marginal bands were prepared by air drying and freeze drying. The marginal bands are composed of tubular elements which vary in diameter from 200 Å to 500 Å, depending on the preparation method. It is suggested that these tubules are part of a general class of microtubules, and that the real diameters of microtubules are approximately twice those observed in fixed and sectioned material.
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  • 170
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    The @Anatomical Record 150 (1964), S. 417-421 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rudimentary cilia were found on fibroblasts of a human brain tumor examined in the electron microscope. These cilia apparently have their origin in the centrioles of the fibroblasts; both centrioles appear to be involved in formation of cilia in most instances observed. Because of their atypical appearance and confinement by adjacent cell processes, it is unlikely that these cilia can perform any useful motor function for the cell. The cilia may serve as an expression of the morphogenetic potential of centrioles.
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  • 171
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ova were removed from donors 63-68 hours after ovulation and placed in the uteri of pseudopregnant recipients of the same pregnancy age. In transfers of ova from young (2 1/2-6 month) to old (14-18 month) golden hamsters, only 8.3% developed into term fetuses. From old donors to young recipients, 4.5% of ova developed to term fetuses. In transfers of ova from young donors to young recipients, 49.2% of ova developed into term fetuses. There was no significant difference in percentage of fetal development between the two groups containing old females. The difference between these and the control (young to young) group was highly significant.There was a significantly lower number of ova recovered from old donors. At time of transfer, over one-sixth of the ova from old donors consisted of only one to four cells. At seven and one-half days, embryos developing from ova of old donors in uteri of young recipients were retarded and malformed.It is concluded that while the uterine environment in old hamsters appears inadequate to foster normal fetal development, there are also fewer ova ovulated in old females and these ova more frequently develop abnormally.
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  • 172
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Development of that part of the external ear which protects the external auditory meatus appears to be similar in all animals since the hillocks of His have been described for reptiles, amphibia, and birds as well as mammals. Disagreement exists as to the origin and fate of the various hillocks, especially tragus and antitragus. Most observations of the developing ear have begun with the formation of the hillocks at which time the tragus is situated on the mandibular arch. Because of the rapid early growth, transitional steps from the open branchial groove to this stage have not been described previously. The retarded growth of the dachs rabbit makes it possible to see intermediate steps. Here it is shown that the entire auricle arises from an intact and continuous primordium, with the tragus arising from the hyoid arch and migrating to the mandibular bar in the normal course of cell proliferation and differentiation. Variations in normal and heterozygous animals can be found in the relative rates of development of the surrounding areas.
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  • 173
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    The @Anatomical Record 148 (1964), S. 15-27 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The A-V node of the dog is composed of closely interwoven fibers which frequently connect with each other within a sparse collagen framework. Fibers from the interatrial septum enter the posterior half of the node along its superior and posterior margins, while the anterior half of the node is isolated from such connections. In addition there are fibers from the interatrial septum which bypass these marginal atrionodal junctions and instead enter the convex surface of the node, which lies beneath the right atrial endocardium. These bypassing fibers circumvent most of the interweaving fibers within the A-V node. At the posterior margin of the node, lying between it and the coronary sinus, there are ganglia and nerves, with the nerve fibers extending anteriorly through the A-V node into the A-V bundle. The only major anatomic difference between the human and canine A-V nodes is their arterial blood supply, that of man being derived from one source originating from the right coronary artery and that of the dog being from two sources, both of which originate from the left coronary artery.
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  • 174
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The excessive growth of fatty tissue around atrophic organs has been described in humans as “hypertrophy ex vacuo”. Since adipose tissue receives its blood supply from smaller branches of arteries which also supply organs or muscles, it has been suggested that increased amounts of blood could be diverted from the inactive organs to the surrounding fatty tissue and promote its growth. This theory was investigated, using rats and mice of different strains. One testis, the artery of which also supplies the epididymal fat pad, was removed, and the animals sacrificed 3-4 weeks later. Growth of the affected fat pad in both rats and mice of normal body composition was significantly reduced. Removal of the testis in adult yellow-obese mice during periods of weight gain produced a moderate but significant weight increase of the affected fat body.
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  • 175
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    The @Anatomical Record 148 (1964), S. 115-119 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: NOrmal and atypical follicular structures were classified and counted in the ovaries of guinea pigs 3 to 11 weeks of age. The per cent of primary and secondary follicles remain almost constant throughout the period of observation. Corpora lutea appeared at the fifth week and reached what appeared to be the mature level by the sixth week. The per cent of follicles having polynuclear ova reached an early peak at four weeks and decreased sharply at five weeks postpartum. The per cent of polyovular follicles increased to a low peak at nine weeks and decreased in frequency thereafter. The frequencies of atretic follicles with polynuclear ova and of quency thereafter. The frequencies of atretic follicles with polynuclear ova and of atretic polyovular follicles were significantly higher than that of atretic normal follicles. The results of this study were compared to simular observations on the Swiss mouse, Golden Hamster and Sprague-Dawley rat. The data were discussed in relation to gonadotripic hormone levels.
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  • 176
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    The @Anatomical Record 148 (1964), S. 149-159 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A marginal sinus, formed by the anastomosed terminations of white pulp capillaries, bounds the inner edge of the marginal zone. The endothelial lining on the nodular surface is complete. Pores appear along the lateral aspect thus allowing blood to pass freely into the interstices of the marginal zone. A dense aggregation of Marshall's metalophil cells occurs in the region of the marginal sinus. Such cells are lacking in the marginal zone.Intravenous injections of particulate matter were used to determine the course of blood flow and phagocytic potential. Ten minutes after injections, particles were found to be dispersed among the cells of the marginal zone. None were found in the marginal metalophils. In four hours the particles had cleared the marginal zone and were within red pulp macrophages. In a few rats, the marginal metalophils reacted positively to tests for iron. It is concluded that marginal metalophils represent a large reserve of potential phagocytes situated at a point where blood first leaves capillaries to enter tissue interstices.
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  • 177
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    The @Anatomical Record 148 (1964), S. 197-202 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This report on the early human ilium is based on the Streeter collection, Carnegie Institution of Washington, Department of Embryology, Baltimore. The five phases in chondrogenesis, described by Streeter for the humerus ('49) and the horizons (Streeter, '42) are used in the description of the changes observed in the ilium. From horizon XV (7-8 mm, 30-32 days) to horizon XVIII (14-16 mm, 36-38 days) component cells of the ilium are mesoblasts. In horizon XIX (17-20 mm, 38-40 days) young cartilage cells have appeared. Both phase 1 and phase 2 cells appear in horizon XX (21-23 mm, 40-42 days), the former characterized mainly by the first appearance of intercellular material, the latter by their slender shape and arrangement in tiers. Phase 3 cartilage cells, cuboidal with many vacuoles, appear in horizon XXI (22-24 mm, 42-44 days). Increasing vacuolization of the cytoplasm of cells is noted throughout horizons XXII and XXIII until, in horizon XXIII (28-30 mm, 46-48 days) total disintegration of cells, a feature of phase 5, is evident. Thus the phases of cartilage differentiation described for the humerus are present also in the ilium.
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  • 178
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Intimal proliferation in dog coronary arteries occurred in the vessel having the greatest range of motion. Within an involved segment of the artery, intimal proliferation could be related to the anatomy of the muscle bundles. Arterial muscle bundles originate from the adventitia, spiral around the artery and insert distally into the adventitia. The adventitia and the internal elastic lamina course along the long axis of the artery and are connected by elastin-collagen fibers. Intimal thickening developed under the muscle insertion sites in regions where the adventitia was apparently placed under tension. In these regions, the net force of a muscle bundle contraction increases and shearing would occur between a muscle bundle at its insertion site and the remaining bundles gliding past it. This local shear force would then be transmitted to the internal elastic lamina through the elastin-collagen fibers which connect it to the adventitia. Deformation and apparent injury of the internal elastica would follow and cause a fibrous tissue response. In the smaller arteries and arterioles, intimal proliferation occurred most prominently in the vessels supplying a mobile structure, i.e., anterior papillary muscle of the left ventricle.
    Additional Material: 17 Ill.
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  • 179
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The luminal and glandular epithelia of the rat uterus during delayed implantation have been studied with the electron microscope. The tall columnar cells of the luminal epithelium are characterized by a distinct zonation of organelles. On the free surface there are short microvilli and occasional cytoplasmic extrusions, with terminal bars joining adjacent cells at their apical margins. Beneath the free surface are numerous pinocytotic vesicles and mitochondria. Above the nucleus are a prominent Golgi apparatus with large dilated vacuoles, and numerous dense inclusion bodies. Large clusters of lipid droplets and aggregations of mitochondria occupy the basal zone of the cell.The apical portion of the gland cell has fewer pinocytotic vesicles and the Golgi is smaller than in the luminal epithelial cells. Dense bodies are numerous above the nucleus. The basal portion of the gland cell is devoid of lipid, but there are often one or more enlarged mitochondria. Although a dense PAS+ substance fills the lumina of the glands, there is no obvious evidence of secretory activity in the glands during delay.The relationships of the epithelia to the environment of the blastocysts during delay is discussed.
    Additional Material: 7 Ill.
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  • 180
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    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 148 (1964) 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 181
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Experiments reveal that growth reactions may be evoked in amputated forelimbs of fully adult Rana pipiens and R. catesbiana by electrical stimulation of the normal limb nerve supply. The evoked growths were generally small cones, 5-10 mm in length; none developed into limbs of normal size and morphology, but some formed digits and cartilage and developed striated muscle. The combined influences of trauma, effected by salt treatment, and electrical stimulation of the nerve produced more growths than trauma or stimulation alone.
    Additional Material: 27 Ill.
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  • 182
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The area of new cell formation, the rate of proliferation, and the manner in which cells are lost from the intestinal epithelium in the turtle, Chrysemys picta, were studied using a cell labeling technique with tritiated thymidine and mitotic inhibiting technique with colchicine.In the intestinal epithelium of Chrysemys picta, cellular proliferation was observed to occur in the layer of cells adjacent to the basement membrane. Mitosis occurred throughout the epithelium at a fairly low rate (mitotic index 0.0016). In the upper intestine fewer labeled cells were found in the apical one-third of a villusfold than in either the mid or basal one-third. The time required to renew the intestinal epithelium, was estimated to be about eight weeks in winter turtles maintained at 20° to 24°C.Cell loss from the system appeared to be due to a randomly located movement of cells lumenward from the basement membrane.Mitotic activity in cold torpid turtles (11°C ambient temperature) appeared to be completely suppressed as shown by a lack of incorporation of tritiated thymidine and an absence of mitotic figures in the intestinal epithelium.The term villi-folds was proposed to provide a category for those animals possessing structures having characteristics both of villi and of folds as found in C. picta.
    Additional Material: 14 Ill.
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  • 183
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Conventional radiographs of lungs of calves which had been injected post mortem with barium sulphate could not be used to demonstrate vessels smaller than 50 μ in diameter. To overcome this difficulty, frozen sections 50-150 μ thick were floated on to high resolution photographic plates and contact microradiographs made using soft radiation. The resultant microradiogram was then viewed and photographed with the light microscope. It was possible to visualize the smaller arterioles and the capillary networks around the alveoli. The technique is simple and gives reproducible results.
    Additional Material: 5 Ill.
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  • 184
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Fifty day old Sprague-Dawley rats were given sodium fluoride (50 mg/kg) and killed at times between one half hour and 32 days after injection. Transverse sections of the upper incisor 40 μ thick were radiographed, demineralized and stained with hematoxylin and eosin. The response seen in hematoxylin and eosin stained material agreed with previous descriptions of the “calciotraumatic response” and consisted of three components. However, in the radiogram only two components could be distinguished: an external hypermineralized zone and an adjacent hypomineralized zone. The radiographic response was first recognized four hours after injection as a narrow zone of hypermineralized dentin at the dentin-predentin junction. When the radiograms were compared to the H and E stained sections in animals killed up to 19 hours after injection, two important features were observed: (1) the width of the predentin had increased markedly, and (2) the hypermineralized zone had increased to its final width. This was interpreted as concurrent formation of both hypermineralized and hypomineralized components. At 24 hours normal dentin formation had resumed. Since both components were forming simultaneously it is believed that sodium fluoride does not cause the response by interfering with the humoral mechanisms of mineralization, but, rather, its effect is most likely on collagen or ground substance.
    Additional Material: 13 Ill.
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  • 185
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    The @Anatomical Record 148 (1964), S. 485-497 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The secretion of protein by the acinar cells of the pancreas occurs in two steps: the synthesis of the protein from amino acids and its migration through the cell to the acinar lumen. Since very soon (one minute) after the injection of leucine-H3 the labeled protein is found mainly in the ergastoplasm of the basal half of the cell, protein synthesis is believed to occur in this location. At later intervals the labeled protein is found in the Golgi complex (10 and 30 minutes), and then in the zymogen granules of the apical half of the cell (60 minutes). The newly synthesized protein thus migrates from the ergastoplasm to the Golgi complex, and from there as zymogen granules through the apical half of the cell prior to being released in the acinar lumen.
    Additional Material: 7 Ill.
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  • 186
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    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 148 (1964), S. 499-501 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The volumes of parenchymal cells and their nuclei in liver were calculated in white rats, Columba livia (rock pigeon), Calotes versicolor (garden lizard) and Bufo melanostictus (toad); from their optical area and cell thickness. The area was measured from camera lucida drawings. The largest cellular and nuclear volume was observed in rats and smallest in pigeons. The cell volume in toads was similar to that found in rats but they had smaller nuclei. The cellular and nuclear volumes in lizards were smaller than in toads; and in pigeons smaller than lizards. The significant difference, in both cellular and nuclear volumes that has been observed in different classes of vertebrate, cannot be correlated with the evolutionary changes.
    Additional Material: 1 Tab.
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  • 187
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 114 (1964) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 188
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 114 (1964), S. 1-41 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 14 Ill.
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  • 189
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 114 (1964), S. 43-57 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 9 Ill.
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  • 190
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
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  • 191
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 114 (1964), S. 83-105 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 12 Ill.
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  • 192
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 193
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 114 (1964) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 194
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 195
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 12 Ill.
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  • 196
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 5 Ill.
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  • 197
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 1 Tab.
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  • 198
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 4 Ill.
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  • 199
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 8 Ill.
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  • 200
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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