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  • 2000-2004  (14)
  • 1960-1964  (234)
  • 1955-1959  (39)
  • Biochemistry and Biotechnology  (262)
  • Phosphorus
  • Polymerization
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  • 1
    ISSN: 1436-3771
    Keywords: Key words Composite resins ; Polymerization ; Marginal adaptation ; Light intensity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The aim of this study was to evaluate the effect of a rapid photopolymerization method on the marginal integrity of composite fillings. Ninety two-surface cavities were prepared in extracted human molars using the SonicSys preparation system. All cavities were bonded with one bonding agent (Syntac) and filled with a microfilled composite (Herculite XRV), a heavy filled composite (Z100 MP), and an Ormocer (Definite) in two increments. Each increment had a thickness of approximately 1.5 mm and was polymerized using either halogen light or the plasma light of Apollo 95 E. In this process, six trial groups each containing 15 fillings were created. After replicas were made, the test teeth were subjected to a thermocycling process of 2000 temperature cycles (5°C/55°C) followed by a wear simulation of 50,000 stress cycles each with 50 N. This was followed by the creation of a second set of replicas, quantitative margin analysis, and dye penetration. A significant (P〈0.05) influence of the thermomechanical stress and a significant (P〈0.05) influence of the factor ”material” were shown for the margin criterion ”continuous margin” using MANOVA. The factor ”light source” produced no significant influence (P=0.57) on the amount of continuous margin. During the dye penetration, no significant differences between the polymerization methods could be established with the materials Herculite XRV and Z100 MP (H-test according to Kruskall and Wallis). Only with the material Definite was a significantly higher dye penetration observed at the occlusal measuring point in the plasma light group. A rapid polymerization with high light intensity had no adverse effects on the margin quality within the limitations of this in vitro study. However the marginal integrity might have been influenced by the chosen adhesive system/composite resin combination, which does not represent the respective product line. Additionally, further studies ought to show which exposure time is necessary for a sufficient degree of conversion.
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  • 2
    ISSN: 1572-8935
    Keywords: Functional monomers ; Polymerization ; Copolymerization ; Kinetic studies ; Reactivity ratios
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract 1-Cyanoethanoyl-4-acryloylthiosemicarbazide (CEATS) was synthesized for the first time as a new chelating monomer. Its structure was confirmed by both elemental and spectral analyses. Radical polymerization and copolymerization of CEATS was been carried out in dimethylformamide (DMF) in the presence of azobisisobutyronitrile (AIBN) as an initiator. Kinetic studies for the polymerization behavior of CEATS were performed. The complex formation of the CEATS monomer and polymer (PCEATS) with Cu II cation was investigated and its stability constant determined. The rate of copolymerization of CEATS with some conventional monomers, namely vinyl acetate, methyl methacrylate and acrylonitrile, was measured as a function of the mole fraction of the monomers. The reactivity ratios (r1, r2) for the various copolymer systems investigated together with the Q and e values of the CEATS monomer were determined. Moreover, the thermal gravimetric analysis of the prepared polymers and their copolymers with acrylonitrile were also studied.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 32 (2000), S. 328-339 
    ISSN: 1432-0789
    Keywords: Key words Biomass transfer ; Maize ; Phosphorus ; Resin extractable phosphorus ; Triple superphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The effects of organic residues and inorganic fertilizers on P availability and maize yield were compared in a Nitisol of western Kenya. Leaf biomass of Calliandra calothyrsus, Senna spectabilis, Croton megalocarpus, Lantana camara, Sesbania sesban, and Tithonia diversifolia were incorporated into the soil at 5 Mg ha–1 for six consecutive seasons in 3 years and responses compared with those following the application of 120 kg N ha–1, 0 kg P ha–1 (0P); 120 kg N ha–1, 10 kg P ha–1; and 120 kg N ha–1 25 kg P ha–1 as urea and triple superphosphate (TSP); K was supplied in all treatments. Addition of Tithonia, Lantana and Croton increased soil resin-extractable P over that of fertilizer-amended soil throughout the first crop, but the amounts in the former treatments became similar to those for soils amended with inorganic fertilizers for subsequent crops. Addition of Sesbania, Calliandra and Senna had a similar effect on resin P as inorganic fertilizers. Total maize yields after six seasons were tripled by the application of Tithonia compared to 0P, and were higher than those of the Calliandra, Senna, Sesbania and Lantana treatments, and similar only to that of the Croton treatment. P recovered in the above-ground biomass and resin P, immediately after the implementation of the treatments, was higher in the Senna, Sesbania, Croton, Lantana and Tithonia (35–77%) treatments than in the inorganic fertilizer treatments (21–27%). The P content of organic residues, and the soluble C:total P ratio, were the main residue parameters predicting soil P availability and maize yield. All organic residues used in this study can replace inorganic fertilizers for the enhancement of P availability and maize production, while an additional benefit could be obtained from the use of Croton, Lantana and Tithonia.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 32 (2000), S. 435-440 
    ISSN: 1432-0789
    Keywords: Keywords Fertilizer ; Long-term field experiment ; Manure ; Mycorrhiza ; Phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  A pot experiment was conducted with soil from a long-term (74-year) fertilization field experiment to compare the effects of organic and mineral fertilizers on mycorrhiza formation in clover, and mycorrhiza-mediated plant P uptake. Five treatments were selected from the field experiment representing different forms and levels of P. Mycorrhizal effects on plant growth and P uptake were estimated by comparing plants grown in untreated soil containing indigenous arbuscular mycorrhizal (AM) fungi, with plants grown in pasteurized soil. Short-term versus residual effects of fertilizer/manure were also measured by comparing treatments with or without fertilizers added at the start of the pot experiment. Mycorrhiza formation was greatest in soil that had received no P for 74 years, followed by soil having received 30 or 60 Mg ha–1 farmyard manure (FYM), and soil having received 25 or 44 kg P ha–1 in NPK fertilizers. Plant growth and P uptake were severely reduced in the absence of AM fungi for all mineral fertilizer treatments. In contrast, plants growing in soil that had received FYM grew equally well or better when non-mycorrhizal. Recent additions of NK fertilizer and FYM had no effect on mycorrhiza formation, while additions of NPK led to reduced colonization. It thus seems that moderate quantities of FYM have less adverse effects on AM than equivalent amounts of nutrients in NPK fertilizers, a phenomenon that is most likely due to a temporal difference in P availability and its gradual release that balance plant demand.
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  • 5
    ISSN: 1432-0975
    Keywords: Keywords Coral ; Nitrogen ; Phosphorus ; Eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract  The effect of prolonged (9 week) nutrient enrichment on the growth and photosynthetic rates of the zooxanthellate coral Stylophora pistillata was investigated. The main questions were: (1) what is the exposure time needed to induce measurable change in growth rate? (2) which are the concentrations of nitrogen and phosphorus required to cause changes in these rates? (3) what is the recovery potential of the corals after the nutrient stress? For this purpose, three tanks (N, P, NP) were enriched with ammonium (N), phosphorus (P) or both nutrients (NP), respectively. A fourth tank (C) served as a control. The growth of 40 nubbins (10 in each tank) was monitored during four periods: period 1 (nutrient-poor conditions), period 2 (10 μm NH4 and/or 2 μm PO4 enrichment), period 3 (20 μm NH4 and/or 2 μm PO4) and period 4 (nutrient-poor conditions). Period 4 was performed to study the recovery potential of corals after a nutrient stress. During period 1, growth rates remained constant in all tanks. In the P tank, growth rates declined during the two enrichment periods, with a total decrease of 60% by the end of period 3. In the N tank, growth rates remained nearly constant during period 2 but decreased in period 3 (60% decrease). In the NP tank, 50% and 25% decreases were observed during periods 2 and 3. At the end of the recovery period, a regain in growth rate was observed in the N and NP tanks (35 and 30% increase, respectively, compared with the rates measured at the end of period 3) and growth rates returned to 60% of the initial rates. By contrast, in the P tank, there was no regain in growth and a further decrease of 5% was observed. Rates of photosynthesis were often higher during the enriched than the nutrient-poor period (up to 150% increase). Corals with the highest percent increases in maximal gross photosynthetic rate (P g max ) had the smallest decreases in growth rate due to nutrient enrichment. In conclusion, high ammonium (20 μm) and relatively low phosphorus concentrations (2 μm) are required to induce a significant decrease in coral growth rate. The largest reduction was observed with both ammonium and phosphorus enrichment. The decrease in growth rate was rapid following nutrient enrichment, since a 10% decrease or more could be observed after the first week of treatment.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of wood science 46 (2000), S. 143-148 
    ISSN: 1611-4663
    Keywords: α-Pinene ; Phenol ; Formaldehyde ; Polymerization ; Condensation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Synthesis conditions of terpene-phenol-aldehyde resin with a high softening point were studied based on the reactions amongα-pinene, phenol, and formaldehyde. A suitable catalyst system and the best processing conditions were obtained by laboratory experiments. The influence of the raw materials ratios, reaction temperature, reaction time, and catalyst dose on the softening point, yield, and color of the synthesized resin was examined. The physical and chemical characteristics of the resin were determined: softening point ≥140°C (ball and ring method); color value ≤7 (Gardner); acid value (KOH mg/g) 〈1; bromine value (Br2 mg/100g) 〈64; saponification value (KOH mg/g) 〈1; average molecular weight ∼830. The yield of resin was more than 85% (based on the total raw material).
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  • 7
    ISSN: 1615-6102
    Keywords: Allium porrum ; Arbuscular mycorrhiza ; Mitotic cycle ; Phosphorus ; Root apex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Arbuscular mycorrhizal (AM) fungi significantly improve plant growth in soils with low phosphorus availability and cause many changes in root morphology, similar to those produced by increased P nutrition, mainly depending on root apex size and activity. The aim of this work was to discriminate between the morphogenetic role of AM fungi and P in leek (Allium porrum L.) by feeding mycorrhizal and nonmycorrhizal plants with two nutrient solutions containing 3.2 or 96 μM P and examining specific parameters related to adventitious root apices (apex size, mitotic cycle, and RNA synthesis). The results showed that AM fungi blocked meristem activity as indicated by the higher percentages of inactive apices and metaphases in the apical meristem of mycorrhizal plants, whereas the high P supply lengthened the mitotic cycle without blocking the apices, resulting in steady, slow root growth. The possible involvement of abscisic acid in the regulation of root apex activity is discussed.
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  • 8
    ISSN: 1435-5604
    Keywords: Key words Calcium ; Phosphorus ; PTH ; Bone meal powder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the effects of bone meal powder (BEC) on calcium and phosphorus metabolism, a calcium absorption test was conducted using a preparation of calcium carbonate (CAC) as the control drug. A total of 12 healthy volunteers, consisting of 6 younger (aged 20–29 years, 3 men and 3 women) and 6 older (aged 60–69 years, 3 men and 3 women) persons, were subjected to a double-blinded crossover study. Serum calcium (s-Ca) level significantly increased to 105.3% ± 1.9% (P 〈 0.01 vs the basal value; mean ± SD) from the basal value in the BEC group and to 104.4% ± 2.7% (P 〈 0.01) in the CAC group at 3 h post load. Urinary excretions of calcium (u-Ca/glomerular filtration rate, u-Ca/GF) after BEC and CAC load rose to 226.6% ± 154.5% (P 〈 0.05) and 211.1% ± 148.0% (P 〈 0.05), respectively. Serum phosphorus (s-P) levels after BEC load increased to 110.0% ± 15.1% (P 〈 0.05), whereas that after CAC load showed no significant change (99.3% ± 7.9%). On the other hand, urinary excretion of phosphorus (u-P/GF) after CAC load decreased to 60.0% ± 32.4% (P 〈 0.01) and that in the BEC group showed no significant change (92.5% ± 49.5%). The increase in s-Ca led to decrease in serum intact parathyroid hormone (i-PTH) level [77.3% ± 33.4% (P 〈 0.05) for BEC and 69.5% ± 20.3% (P 〈 0.01) for CAC] although s-P was increased by the BEC load. The responses to BEC and CAC administration were compared in the younger and the older groups. The responses in the younger and the older group showed fundamentally the same trends and to the same extent. However, the changes in serum ionized calcium (i-Ca) and i-PTH levels at 1.5 h post load were significantly smaller in the older group than in the younger group (P 〈 0.01; P 〈 0.05). The increment in s-P level after BEC load in the older group was larger than that in the younger group. In conclusion, BEC can modulate not only calcium but also phosphorus metabolism in both younger and older subjects. Further investigations are required to evaluate the effects of BEC on bone density and safety for renal function in long-term observations.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 278 (2000), S. 150-154 
    ISSN: 1435-1536
    Keywords: Key words Hydrolysis kinetics ; Polymerization ; Microemulsions ; Aspirin ; Styrene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Two kinds of chemical reactions were studied in two different microemulsion systems: cetyltrimethylammonium bromide/1-butanol/10 and 25% n-octane/water and sodium dodecyl sulfonate/1-butanol/20% styrene/water. One reaction is a hydrolysis reaction, in which aspirin and 2,4-dinitrochlorobenzene were used as the hydrolysis substrates. The second reaction is the polymerization of styrene, which was initiated by using two initiators, water-soluble K2S2O8 and oil-soluble 2,2′-azobis(isobutyronitrile), and, at the same time, the polymerization of acrylamide, which was initiated by NaHSO3, was also studied. All the hydrolysis reaction experimental results show that the hydrolysis is greatly affected by the structures and the structural transitions of microemulsions. The hydrolysis rates are higher in water-in-oil (W/O) microemulsion media and decrease with the addition of water. The rates increase in bicontinuous (BC) microemulsions and decrease in oil-in-water (O/W) microemulsions. The transition points of the hydrolysis rates occurred at the two microemulsion structural transition points from W/O to BC and from BC to O/W. The polymerization relationships between the conversions of styrene, the molecular weights of polystyrene and the water contents of the microemulsion system were obtained. The effects of microemulsion structures on the sizes of the polystyrene particles and on the molecular weights of the polymers are discussed. Polystyrene particles with diameters of 10–60 nm were observed by microscopy. Our experimental polymerization results show that microemulsions are suitable as media for the production of polymers, the molecular weights and the particle sizes of which can be controlled and predicted by variations in microemulsion structures.
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  • 10
    ISSN: 1434-1948
    Keywords: Hypercoordinated phosphorus ; Hypervalent phosphorus ; Phosphorus ; Diphosphonium compounds ; Ionomers ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Starting from R′R2P (R′ = 8-dimethylamino-1-naphthyl) containing a donor dimethylamino group, the new phosphonium salts [R′R2P(CH2Ph)]+Br- [R = Me (9) or Ph (10)] and [R′R2P(p-CH2C6H4CH2)PR2R′]2+[2Br]2- [R = Ph (12)] have been prepared. An interaction between the N and P atoms is evident from the X-ray crystal structure of 10 the N-P distance being less than the sum of the van der Waals radii of the 2 atoms. The geometry of 10 is that of a monocapped tetrahedron whereas the X-ray crystal structure determination shows essentially regular tetrahedral geometry for the analogous compound without the donor amino group, [(1-Np)Ph2P(CH2Ph)]+Br- (11). Treatment of 1,5-bis(dimethylamino)-2,6-dilithionaphthalene with chlorodiphenylphosphane gave 1,5-bis(dimethylamino)-2,6-bis(diphenylphosphanyl)naphthalene (8) which in the presence of methyl iodide afforded the diphosphonium salt [1,5-bis(dimethylamino)-2,6-bis(diphenylmethylphosphonium)naphthalene]2+[2I]2- (13). Similarly, treatment of 8 with 1 equivalent of benzyl bromide gave the monophosphonium salt [1,5-bis(dimethylamino)-2diphenylbenzylphosphonium-6-diphenylphosphanyl-naphthalene]+[Br]- (14) whereas in the presence of 2 equivalents of the same reagent [1,5-bis(dimethylamino)-2,6-bis(diphenylbenzylphosphonium)naphthalene]2+[2 Br]2- (15) was obtained. The ionomer poly([(1,5-bis{dimethylamino}2,6-bis{diphenylphosphonium}naphthalene)-(P,P-p-xylylene)]2+[2 Br]2-) (16), soluble in liquid SO2, was prepared by treatment of 8 with α,α′-dibromo-p-xylene.
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  • 11
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 2000 (2000), S. 103-112 
    ISSN: 1434-1948
    Keywords: Ab initio calculations ; Ionization potentials ; Phosphorus ; Heterocycles ; Small ring systems ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Ab initio quantum chemical calculations have been used to explore the P3H3 potential energy surface focussing on the ring-chain rearrangements of the three-membered ring in (PH)3 (1), the parent triphosphirane. Relative energies between stationary points were estimated using the QCISD(T)/6-311G(d,p) method based on MP2/6-31G(d,p) geometries and corrected for zero-point contributions. Ring strain, proton affinities, ionization and excitation energies and heats of formation have been evaluated using larger basis sets, e.g. 6-311++G(3df,2p). The cyclic trans-triphosphirane (1a) is the most stable P3H3 isomer and lies about 40 kJ/mol below the open-chain phosphanyldi-phosphene (H2P-P=PH). The decrease of ring strain in three-membered rings when CH2 is replaced by PH is confirmed. Triphosphirane 1a is a virtually strain-free ring and even gains some stabilization relative to three separate P-P single bonds. The reduced ring strain also helps diminish the phosphorus inversion barrier to 224 kJ/mol compared to the monocyclic isomers of (CH2)(PH)2 and (CH2)2(PH). Compound 1a follows a pure ring-opening or a 1,2-hydrogen shift rather than a combined motion pathway, in fundamental contrast with corresponding processes of diphosphirane and phosphirane. This is due to the existence of an open-chain P3H3 phosphorane intermediate stabilized by allylic conjugation. The pericyclic ring-opening of 1a is the most favored process but the energy barrier in the gas phase is about 180 kJ/mol high. Electron density is largely delocalized within the three-membered P3 ring not only in the C3v-symmetric 1b (all-cis) but also in 1a (Cs). The proton affinity of 1a is similar to that of PH3. The proton affinities decrease with n in cyclo-(CH3)3 -n(PH)n and their values were obtained: PA(1a) = 777 ±10, PA(diphosphirane) = 799 ±10 and PA(phosphirane) = 802 ±10 kJ/mol. Heats of formation are evaluated as follows (ΔH°f0 at 0 K in kJ/mol): 1a, 70 ±10; cyclo-(PH)2(PH2)+ (protonated 1a), 821 ±10; diphosphirane, 85 ±10; cyclo-(CH2)(PH)(PH2)+ (protonated diphosphirane), 814 ±10; phosphirane, 86 ±10; and protonated phosphirane, 812 ±10 kJ/mol. All P rings remain cyclic following ionization to the radical cations. Adiabatic ionization energies (IEa) are estimated as: 1a and diphosphirane, 9.3 ±0.3 eV and phosphirane 9.5 ±0.3 eV. The first UV absorption band shifts toward the longer wavelength region on going from phosphirane to 1a. The GIAO/B3LYP computed magnetic shieldings for 1a and related molecules reveal a clear relationship between the narrow bond angles in the rings and their unusually strong magnetic shielding. The similarity of the predicted 31P-NMR signals in 1a and its heteroanalog diphosphirane, (CH2)(PH)2, can be rationalized in terms of a compensation of the carbon-substituent effect (downfield shift) and the bond-bending effect imposed by the ring (upfield shift).
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  • 12
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 2000 (2000), S. 417-421 
    ISSN: 1434-1948
    Keywords: Phosphorus ; Benzynezirconocenes ; Azazirconacyclopentenes ; Exchange reactions ; 1,2-Azaphosphindole ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The first 1,2-azaphosphindoles 14a,b, 15a,b were obtained in a straightforward manner upon heating diphenylzirconocene in the presence of a cyanophosphane, which afforded azazirconacyclopentenes 11a,b which can be reacted with various dichlorophosphanes. The use of the tetrachlorodiphosphane Cl2P(CH2)2PCl2 instead of a dichlorophosphane allowed the preparation of the bis(1,2-azaphosphindoles) 19, 19′. The monosulfur adduct of the azaphosphindole 14a, i.e. 16a, was characterized by X-ray structure analysis. Alkylation of 14a or 16a with methyltrifluoromethane sulfonate occurred selectively on the intracyclic phosphorus atom or on the sulfur atom, giving the salts 20 or 21, respectively.
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  • 13
    ISSN: 1434-1948
    Keywords: Aluminum ; Alkoxides ; Lactone ; Ring-opening ; Polymerization ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Reaction of trialkylaluminum Al2R6 (R = Me, Et) with 2,2′-methylenebis(6-tert-butyl-4-methylphenol) (mbmpH2) gives the dimeric mono(alkyl) complex [Al(mbmp)R]2 with bridging oxygen atoms. Reaction of [Al(mbmp)R]2 with one equiv. of 2-propanol results in the formation of the dimeric isopropoxide [Al(mbmp)(μ-OiPr)]2. Single-crystal X-ray analysis shows a C2h-symmetric structure with a planar Al2O2 core. Monomeric methylbis(2,6-di-tert-butyl-4-methylphenolato)aluminum, AlMe(OC6H2-2,6-tBu2-4-Me)2 (MAD), was found to react with one equiv. of 2-propanol to give a dimeric isopropoxide [AlMe(OC6H2-2,6-tBu2-4-Me)(μ-OiPr)]2 in which the bulky phenolate, instead of the methyl group, has been displaced. According to the single-crystal X-ray structure analysis, the molecule contains a similar Al2O2 core, but the two 2,6-di-tert-butyl-4-methylphenolato ligands are cis- and orthogonally arranged to each other. ε-Caprolactone is polymerized at 50 °C in toluene in a controlled manner by [Al(mbmp)(μ-OiPr)]2, to give poly(ε-caprolactone) with high molecular weights and narrow molecular weight distributions (Mw/Mn 〈 1.50). The low efficiency values (number of polymer chains initiated per aluminum atom) imply that [Al(mbmp)(μ-OiPr)]2 exists in a monomer-dimer equilibrium. The polymerization of ε-caprolactone by [AlMe(OC6H2-2,6-tBu2-4-Me)(μ-OiPr)]2 is faster, but somewhat less controlled.
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  • 14
    ISSN: 1434-1948
    Keywords: Thiophosphites ; X-ray diffraction ; Manganese ; Iron ; Phosphorus ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Thioesters of the phosphorous acid containing cymantrenyl and ferrocenyl substituents at sulfur were obtained for the first time from the reaction of organometallic disulfides with white phosphorus. According to an X-ray diffraction study tricymantrenyl trithiophosphite adopts an unusual asymmetric cis, gauche, trans conformation along the P-S bonds with respect to the phosphorus lone electron pair. Triferrocenyl trithiophosphate possesses a classical propeller-like structure in the solid state which is different from its oxygen analogue.
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  • 15
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 309-319 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The authors describe continuous cultivations of Escherichia coli possessing high penicillin-acylase activity in corn-steep liquor, peptone, and ammonium phenylacetate containing nutrient medium. If the cultivation is performed in the absence of ammonium phenylacetate the enzymic activities of cells from the batch as well as the continuous cultures are very low. The enzymic activity of cells in the batch process was considerably increased by the addition of 0.015% ammonium phenylacetate to the nutrient medium. Further increase of ammonium phenylacetate concentration did not result in any further increase of acylase activity. Continuous cultivation of the bacteria at the above ammonium phenyl-acetate concentration was unsuccessful, as the enzymic activity of the bacteria constantly decreased during the process. On increasing the concentration of ammonium phenylacetate in the medium to 0.15% the authors succeeded in maintaining the enzymic activity of the bacteria at the same level as in the batch process performed at 0.015% concentration, throughout the whole continuous cultivation. At the dilution rate D = 0.5 hr.-1 the concentration of cells in the culture effluent from the fermentor at the steady state was equal to cell concentration at the end of batch cultivation. In relation to the cultivation time the output of cells in continuous cultivation is almost seven times higher as compared with the batch process.
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  • 16
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Steam-sterilizable membrane probes for monitoring the dissolved oxygen level in fermentors, or the oxygen content of gas streams, are described. The probes have a silver cathode, a lead anode, and an acetate buffer as an electrolyte. The membrane is Teflon. The current output of the probes in the absence of oxygen is negligible.
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  • 17
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 6 (1964), S. 191-221 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Distillers' yeasts, strains of Saccharomyces cerevisiae, although capable of sexual reproduction, in distillery practice reproduce asexually, by budding. A cell may bring forth another one in 50 min. With every ounce of whiskey produced, 30 billion new cells come to existence. Within a few days, in 4-8 propagation stages, a test tube full of culture will populate 100,000 gallons beer with 150 million cells per ml. Rate of reproduction, the number of new cells per each original cell varies from 5 to 50 in the various propagation stages. The number of new cells produced in a given nutrient is independent from the number of initial cells; and the utilization of the nutrient increases with the dilution of the substrate. Although distillers' yeasts may reproduce at such extremes as 1-46°C, 2½-10½ pH, presence of 0-15% alcohol by volume, and 0.1-25% sugar content, in distillery practice the factors are so selected to maintain conditions close to the optimum. When placed in the nutrient the initial cells will measure the chemical and physical characteristics of the new living space and the cell population, and will prepare a design of reproduction best suited to the conditions. The design includes a symmetry in the grouping of the cells and a rhythmic timing in starting new buds. Each healthy cells is biologically equal to the others and is capable of performing all functions characteristic of the strain. In spite of the sensitive coordination system between the individual cells that regulates their activity, marked differences exist among the cells to the degree of cell individuality. Distillers yeasts are superbly equipped to live and reproduce.
    Additional Material: 23 Ill.
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  • 18
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    Biotechnology and Bioengineering 6 (1964), S. 469-471 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It was found that moderate pressures (16.0 to 43.1 atmospheres) perceptibly decreased the rate of α-amylase catalyzed starch hydrolysis. The decreased rate was apparently due to pressure induced deactivation of the enzyme.
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  • 19
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    Biotechnology and Bioengineering 6 (1964), S. 491-496 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 20
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    Biotechnology and Bioengineering 6 (1964), S. 223-234 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of performing electrophoretic separations in a thin horizontal layer of continuously flowing buffer has been developed. The theoretical considerations that make possible the use of a thin layer for stabilization against convection effects, without the assistance of a packing material, are discussed. The apparatus is described together with an example of its performance, and a comparison with that of other workers.
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  • 21
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    Biotechnology and Bioengineering 6 (1964), S. 235-240 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The operation and construction of a liquid-liquid chromatography apparatus containing differentially permeable partions is described. The method is of value for the separation of heat sensitive and nonvolatile solutes.
    Additional Material: 2 Ill.
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  • 22
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    Biotechnology and Bioengineering 6 (1964), S. 367-379 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous flow-type reactors have been used to study the kinetics of biological systems for quite some time. For continuous media sterilization, tubular flow reactors are particularly useful being simple in character and easy to control. However, one aspect quite often neglected in sterilization calculations is the residence time distribution of the reactor system. Serious errors in estimating the degree of bacterial destruction can be encountered if the residence time distribution is neglected; especially when a high degree of destruction is desired. This paper reports a study made to characterize and use the residence time distribution of a tubular reactor in the interpretation of high-temperature, short exposure time data for inactivation of Bacillus stearothermophilus spores. Mathematical models accounting for the residence time distribution of the tubular reactor have been proposed and employed to obtain high-temperature death-rate data.
    Additional Material: 8 Ill.
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  • 23
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A cultivation apparatus made up from six small (80-800 ml.) glass units with independent pH-, aeration-, and foam control is described. Exchangeable attachments made it possible to run the unit both batchwise and continuous and to connect up the units in various fashions.
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  • 24
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two sources of oxygen for man in closed-cycle space system environment have been considered in previous studies: (1) photosynthesis using algae, and (2) electrolysis of water. The latter system appears to be the most promising from the standpoints of energy and weight requirements and ability to operate in a zero gravity field. The surplus hydrogen produced by the electrolysis of water may be utilized together with waste carbon dioxide, part of the oxygen, and waste urea by bacteria of the genus Hydrogenomonas to produce cellular protein which might be used as a source of food. A continuous culture system for the propagation of hydrogen-fixing bacteria consists of a baffled borosilicate glass culture vessel provided with an impeller, a reservoir vessel for the culture medium, and an overflow vessel for collecting the bacterial cells removed from the culture vessel. Complete feedback control of all parameters affecting growth can be provided by hydrogen, oxygen, and carbon dioxide sensors, and a pH electrode in the culture medium. In addition, total pressure is monitored. Cell density is controlled in the optimum range by means of a photoelectric cell which dictates the amount of fresh medium to be added and the amount of cells to be removed. Operating data indicate that some of the key parameters are the ratio of hydrogen, oxygen, and carbon dioxide in solution. The harvested bacterial substance is high in protein, which contains all the essential amino acids.
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  • 25
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    Biotechnology and Bioengineering 6 (1964), S. 3-4 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 26
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    Biotechnology and Bioengineering 6 (1964), S. 50-52 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 27
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    Biotechnology and Bioengineering 6 (1964), S. 63-64 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 28
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    Biotechnology and Bioengineering 6 (1964), S. 71-72 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 29
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    Biotechnology and Bioengineering 6 (1964) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 30
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    Biotechnology and Bioengineering 6 (1964), S. 147-158 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The equipment and operational techniques are described which were found suitable to control pH in the range 6.8-7.8 pH, within ±0.03 pH units of the desired value, at cell concentrations up to a maximum of 2.5 × 106/ml. The results of batch growth of a suspension strain of the BHK cell (clone 13) under conditions of controlled pH are given and the significance of these results is discussed.
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  • 31
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    Biotechnology and Bioengineering 6 (1964), S. 127-146 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sugar molasses of high specific activity is prepared by photosynthesizing C14O2 into sugar in a leaf of the Canna plant and then extracting the sugar. When T. utilis is grown in such a medium about 45% of the radioactivity is incorporated into the yeast cell; the remaining radioactivity is carbon dioxide and volatile organic compounds. The cells are now randomly labeled in all carbon compounds. Distribution of C14 in the major cell components, such as protein, polysaccharide, and nucleic acids is shown.Nucleic acid is extracted from the cells and subject to chemical and enzyme action, for the preparation of randomly labeled ribonucleotides. Proteins are isolated and hydrolyzed to yield high specific activity amino acids, up to 150 mc. per millimole. Similarly polysaccharides are also isolated and from them high specific activity glucose and mannose are obtained by acid hydrolysis.By growing T. utilis in a medium containing C14-8-adenine and C14-2-uracil, these bases are about 90% incorporated into the nucleic acids of the cell. Using the same methods described for the uniformly labeled nucleic acid only base labeled nucleosides and nucleotides are prepared.Preparative procedures for the isolation of some of these compounds are discussed. The latest biochemical techniques for their purification are described. Methods for assaying for radiochemical purity and problems concerining stability of high specific activity compounds are discussed.
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  • 32
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    Biotechnology and Bioengineering 6 (1964), S. 159-165 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method has been devised for the estimation in fermentation media, of 1-dehydro-17-α-methyltestosterone in the presence of 17-α-methyltestosterone. This method involves the extraction of the steroids with chloroform, and spectrophotometric measurement of the chromogen formed by a specific color reaction with sulfuric acid and 1-dehydro-17-α-methyltestosterone. The fermentation liquor and 17-α-methyltestosterone do not interfere. With this rapid (30 min.) method, quantitative tests on the progress of the steroid transformation can be made at frequent intervals during one fermentation run, these tests yielding the necessary information for an adequate control of the optimum conditions of production.
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  • 33
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    Biotechnology and Bioengineering 6 (1964), S. 167-171 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Solubilization of proteins from cereals, legumes, and oil seed cakes by alkali peptization and isoelectric precipitation is adversely affected if the source material is pretreated by ethanol or cooked under pressure. The degree of hydrolysis by trypsin of protein isolates obtained from unautoclaved materials is, however, considerably raised by heat treatment prior to enzymatic digestion.
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  • 34
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    Biotechnology and Bioengineering 6 (1964), S. 173-190 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 2000 l. 1 m. deep mass culture of algae was operated with continuous stirring, as an open system. The system behaved as an ecological unit selecting the most favored species. The ecological conditions could be modified by stirring speed and pattern in the tank. Methods for improving yields and utilization of CO2 are described. Assessment of algal species for suitability in mass cultures is discussed. Yields obtained were 13 g. dry matter/sq. m. illuminated area/day.
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  • 35
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    Biotechnology and Bioengineering 6 (1964), S. 245-245 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 36
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    Biotechnology and Bioengineering 6 (1964), S. 247-270 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of ambient pressure and ultrasonic power on the disintegration of yeast suspension have been investigated. The results obtained are, in the main, consistent with the theory that cell breakage is primarily a phenomenon dependent on producing gaseous cavitation in the medium. The importance of the experimental results and techniques applied to Commercial cell disintegrators is briefly discussed. A simple flow system is described which is easily attached to probe-type disintegrators. The use of a crystal pickup for tuning and control purposes is described.
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  • 37
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    Biotechnology and Bioengineering 6 (1964), S. 241-242 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 38
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    Biotechnology and Bioengineering 6 (1964) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 39
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    Biotechnology and Bioengineering 6 (1964), S. 271-283 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aeration and agitation conditions in 250 l. baffled fermenters with different impeller diameters, speeds, and air flows were studied during fermentations for the production of rifamycin, a new antibiotic produced by Streptomyces mediterranei. Dissolved oxygen concentration was continuously measured and the courses of power input, oxygen diffusion rate QO2, dry weight, viscosity, pH, sugar utilization, and antibiotic titer determined. Higher antibiotic yields occurred when the oxygen demand of the culture was satisfied and an excess of dissolved oxygen was still present during a critical period of the fermentation between the 50th and the 80th hr. To meet this requirement a power input of about 3.0 w./l. and an air flow ranging from 0.8 to 1.5 l./l./min. were found to be necessary.
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  • 40
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    Biotechnology and Bioengineering 6 (1964), S. 285-297 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Semicontinuous sulfide fermentation has been conducted with distillery spent liquor as the source of carbon employing a strain similar to Desulphovibrio rubentschikii. Mineral barites and gypsum have been used as the hydrogen acceptors during the studies. Results reveal that mineral barites can also react very favorably as hydrogen acceptors. Sulfates have been used both in powdered (200 mesh) and lumpy (1/4-1/2 in. size) forms. When applied in lumpy form in a packed tower, both the mineral sulfates produced higher yields of sulfide compared to their corresponding finely pulverized states. Thus, under similar conditions, fermentation conducted with pulverized gypsum showed a yield of 450-550 p.p.m. hydrogen sulfide, whereas the yield was as high as 750-1025 p.p.m. when gypsum lumps were used as a packing. Corresponding figures with barites are 300 and 540 p.p.m., respectively. Continuous sulfide fermentation has also been conducted in a packed tower containing lumpy gypsum as the packing under total recirculation. Daily buildup of sulfide was observed to be as high as 180 mg./l. of fermenting medium. Appreciable reduction in COD of the effluent was observed during the above studies. Thus, with barites, per cent COD reduction values were 30-35, 45-50, 60-65, and 75-80 with retention periods of 10, 15, 25, and 35 days, respectively. With gypsum, per cent COD reduction was 50-70 with 10 days's residence time. The effects of the retention period on sulfide yield and volatile acid content were also studied. The longer the retention period, the higher is the sulfide yield and the lower the volatile acid content of the discharge becomes. Studies were also conducted to find the correct composition of volatile acids in the discharge, as well as the levels of their concentration during continuous feeding and discharge over a number of days. It has been observed that during 18 hr. of fermentation in a batch, volatile acid concentration increased from 6.76 m. to 15.01 mmoles per liter of medium of which 10 mmoles are acetic, 0.833 mmoles butyric, and 4.16 mmoles propionic acid. Studies on soluble and insoluble sulfate balance have also been made in a synthetic medium containing calcium acetate as the substrate. Unaccounted for sulfates were negligible and a deviation between the actual and calculated sulfide yield did not exceed 2%. It is also observed that, when fermentations are conducted in sulfate packed columns, the color of the effluent becomes paleyellow containing very little suspended and volatile matter compared to the deep-black color of the emuent emerging from a ferrnenter fed with pulverized sulfates. This liquor contains 0.5271% soluble ash (computed as potash) and might be a good source of potash.
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  • 41
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    Biotechnology and Bioengineering 6 (1964), S. 10-11 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 42
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    Biotechnology and Bioengineering 6 (1964), S. 22-22 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 43
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    Biotechnology and Bioengineering 6 (1964), S. 36-49 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 44
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    Biotechnology and Bioengineering 6 (1964), S. 59-62 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 45
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    Biotechnology and Bioengineering 6 (1964), S. 73-81 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 46
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    Biotechnology and Bioengineering 6 (1964), S. 8-9 
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  • 47
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    Biotechnology and Bioengineering 6 (1964), S. 18-21 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 48
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    Biotechnology and Bioengineering 6 (1964), S. 26-28 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 49
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    Biotechnology and Bioengineering 6 (1964), S. 53-56 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 50
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    Biotechnology and Bioengineering 6 (1964), S. 65-68 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 51
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    Biotechnology and Bioengineering 6 (1964), S. 329-345 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: During investigations of submerged fermentation of penicillin and chlortetracycline, we reached the conclusion that production in relation to oxygen transfer is relatively uninfluenced by the composition of the fermentation medium. The maximum penicillin production, using the strain Penicillium chrysogenum NG in flasks on a shaker was uniformly achieved in all investigated media with an oxygen transfer rate of 0.81; and in chlortetracycline, using strain Streptomyces aureofaciens BMK-11, with an oxygen transfer rate of 0.59 ml. O2/ml./hour. The primary factor which influences the antibiotics production is the transfer of oxygen into the liquid. The absolute volume of production is, however, partly influenced by mechanical conditions of fermentation and the composition of the medium.
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  • 52
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    Biotechnology and Bioengineering 6 (1964), S. 361-365 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability of Bacillus subtilis to accumulate guanosine 5′-monophosphate (5′-GMP) extracellularly was demonstrated by isolation of 85% pure 5′-GMP from the broth. In addition, a compound with the properties of guanosine 5′ diphosphate was obtained from the same broth.
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    Biotechnology and Bioengineering 6 (1964), S. 5-5 
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  • 54
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    Biotechnology and Bioengineering 6 (1964), S. 12-13 
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    Biotechnology and Bioengineering 6 (1964), S. 82-84 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 56
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    Biotechnology and Bioengineering 6 (1964), S. 87-125 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
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  • 57
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    Biotechnology and Bioengineering 6 (1964), S. 243-243 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 58
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    Biotechnology and Bioengineering 6 (1964), S. 23-25 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 59
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    Biotechnology and Bioengineering 6 (1964), S. 299-307 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A soil isolate, HD-5, identified as closely resembling Candida intermedia, was grown on normal alkanes ranging from dodecane (C12) to octadecane (C18). The growth rate of the organism increased with increasing length of the alkane chain. The shortest generation time was 4.5 hr. with octadecane as the carbon source. Cell yields of 82% were common with even numbered alkanes from C14 through C18. The cell yields appeared to decrease with shorter chain (C12 or below) and odd-numbered chain alkanes. The cellular nitrogen ranged from 6.9 to 7.5% and the highest lipid content found was 10.3% (with C17).
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  • 60
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Various methods for the removal of extraneous matter from vaccinia virus suspensions are described and compared. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high virus titers in embryonated eggs and high infectivity for mice. Although both processes resulted in products with the same titers, the differentially centrifuged product was approximately tenfold more purified as shown by the percentage solids of the suspensions before freeze drying.
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  • 61
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    Biotechnology and Bioengineering 6 (1964) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 62
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    Biotechnology and Bioengineering 6 (1964), S. 347-360 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The oxygen uptake rate to suspended cells of Pseudomonas ovalis was measured in two ways using the same cell suspension. Initially the rate was found by measuring the rate of production of gluconic acid by cells suspended in a nitrogenfree, aerated medium. Then, an oxygen electrode was used to measure the rate of transfer of dissolved oxygen to cells suspended in a liquid that was being agitated but not sparged. These rates were markedly different. It was found that agitation affected the oxygen transfer rates in aerated solutions at dissolved oxygen concentrations well above the critical level, but had no affect on the oxygen uptake rates by cells suspended in an unsparged but agitated medium. The data suggested that an additional path existed for oxygen transfer. This alternate route, parallel to the conventional pathway of oxygen transfer, becomes operative when the liquid films surrounding the cells and bubbles merge. The resulting shorter path presents a mechanism for direct transfer of oxygen which increases in importance as the gas-liquid interfacial area increases.
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  • 63
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    Biotechnology and Bioengineering 6 (1964), S. 381-401 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A unit of four fermentors, which is specially designed for batch and continuous cultivation of pathogenic microorganisms in vaccine preparation, is described. Each unit consists of a panel with controls and recorders. The fermentor itself consists of a stainless steel lid, to which a spherical or cylindrical glass vessel is attached. Holding volumes are from 1 to 100 l. (3 and 7 l. being mostly used) and can be obtained by simply exchanging the vessels which have similar flanges.Interchangeability, not only of the glass vessels, but also of the stirrers, pipes, pH electrodes, etc., together with variable control of temperature, stirrer speed, air or gas flow, and pH value gives the unit a maximum of versatility.
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  • 64
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    Biotechnology and Bioengineering 6 (1964), S. 403-418 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Methods for composting small quantities of organic materials have been developed in order to be able to better study the composting process. A composting bin was developed which holds 27 cu. ft. of compost and which provides uniform conditions of temperature, moisture, and aeration within the compost mass. A system for indoor composting of less than one cubic foot of material has been developed and is described. The methods have been shown to be reliable and reproducible. The composts were evaluated for their suitability for mushroom growing and were found to give high yields of mushrooms.
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  • 65
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    Biotechnology and Bioengineering 6 (1964), S. 473-490 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 7 Ill.
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  • 66
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    Biotechnology and Bioengineering 6 (1964) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 67
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    Biotechnology and Bioengineering 6 (1964), S. 6-7 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 68
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    Biotechnology and Bioengineering 6 (1964), S. 14-17 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 69
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    Biotechnology and Bioengineering 6 (1964), S. 29-35 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 70
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    Biotechnology and Bioengineering 6 (1964), S. 57-58 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 71
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    Biotechnology and Bioengineering 6 (1964), S. 69-70 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 72
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    Biotechnology and Bioengineering 6 (1964), S. 243-243 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 73
    ISSN: 0570-0833
    Keywords: Stereospecific polymerization ; Polymerization ; Polycyclopentene ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The polymerization of cyclo-olefins, like that of olefins with an internal double bond, is sterically hindered. Within recent years it has become possible to prepare copolymers of these compounds with ethylene, with the aid of anionic coordination Ziegler catalysts. This copolymerization always results in cis-opening of the double bond. Despite the steric hindrance, cyclobutene and cyclopropene have also been homopolymerized, with opening of the double bond. - Rather surprisingly, the best catalysts for homopolymerization of cyclopentene are those which exhibit low activity in the polymerization of ethylene. Ring cleavage occurs with MoCl5/Al(C2H5)3 to give the cis-polypentenamer, whereas WCl6/Al(C2H5)3 gives the trans-polypentenamer. Both polypentenamers exhibit elastomeric properties. - Evidence from infrared spectra and oxidative degradation indicates that the monomer units in the trans-polypentenamer are linked head-to-tail. It is presumably the single bond adjacent to the double bond that is broken. Using X-ray methods at -50 °C, it was possible to determine the crystal structure of the crystalline trans-polypentenamer at about 400% elongation.
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  • 74
    ISSN: 0570-0833
    Keywords: Tetradentate ligands ; P ligands ; Arsenic ligands ; Phosphorus ; Arsenic ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The effect of ligand shape on coordination numbers and geometry of coordination compounds has been studied using two tetradentate ligands containing phosphorus and arsenic. These tend to form five-coordinate complexes with trigonal bipyramidal structure [Fe(II), Co(I), Co(II), Rh(I), Ni(II), Pd(II), Pt(II)], but can also form six-coordinate complexes with octahedral structure [Fe(II), Ru(II), Os(II), Co(III), Rh(III), Pd(IV), Pt(IV)]. The magnetic properties and the stereochemistry of the complexes are explained by ligand field theory which predicts that, for metal ions with d6-electronic configuration, a low overall field strength should favor the formation of five-coordinate paramagnetic complexes while a high overall field strength should favor the formation of six-coordinate diamagnetic complexes. - This work provides further indication that the tendency of polydentate ligands to use all the potential donor atoms is not as pronounced as is generally believed. This is shown by the isolation of chromium(III) complexes in which the tetradentate ligands are, in fact, acting as tridentate, and by the isolation of mercury(II) complexes where only two of the four donor atoms of the polydentate ligand are actually bonded to the central metal atom.
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  • 75
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 76
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    Biotechnology and Bioengineering 5 (1963), S. 309-330 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Biological purification of water pollutants depends upon a complex balance of organisms and environment conducive to effective metabolism. The bench scale test is useful to determine effects of a given waste water on that balance and to suggest controls promoting optimum purification. Applicability of laboratory results depends, in part, upon an experimental design similating anticipated operating conditions. This paper discusses advantages and limitations of historical bench-tests as a guide for design of tests giving data that are more useful during full-scale operation.
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  • 77
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    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 78
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 50-gal. growth chamber is described together with its air filter and humidifier. The unit was designed especially for the production of Neurospora crassa, and was found to be effective in producing bulk amounts of mycelia with a high specific activity of tryptophan synthetase.
    Additional Material: 3 Ill.
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  • 79
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    Biotechnology and Bioengineering 5 (1963), S. 87-89 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Addition of the S-2-hydroxyethyl-, S-2-chloroethyl-, S-n-propyl-, and S-iso-propyl-derivatives of homocysteine, and methoxinine to S. aureofaciens (ATCC 13900) fermentations resulted in production of 7-chloro-6-demethyltetracycline in addition to the normally synthesized 7-chlortetracycline.
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  • 80
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    Biotechnology and Bioengineering 5 (1963), S. 123-129 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of inoculating nutrient surfaces uniformly with bacterial cells has been developed. The method is based on controlled exposure of samples in a humidified chamber to a spray produced from a bacterial suspension. Factors affecting uniformity of inoculation (ionic strength of suspension, force of spray, distance between spray nozzle and sample, and exposure time) were studied in detail. Under optimum condition using agar medium in Petri plates, differences between total counts for six plates inoculated simultaneously, or between counts for individual 2 cm.2 areas taken at any location on any of the plates, were not statistically significant at the 5% level. The coefficient of variability for counts on 2 cm.2 areas renged from 8 to 12% between tests of 216 counts each.
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  • 81
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    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 82
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    Biotechnology and Bioengineering 5 (1963), S. 185-192 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: By growing Aspergillus parasiticus (C.M.I. 15957) under controlled conditions on sterilized peanuts an average of 265 mg. of aflatoxin per kilogram of peanuts has been produced and subsequently isolated. Other strains from the A. flavus-oryzae group gave lower yields of aflatoxin and one such strain gave aflatoxin from which certain normal components were absent. The aflatoxin produced on sterilized peanuts by any particular strain of A. flavus-oryzae was shown by thin-layer chromatography to contain the same major components as were produced by that strain on unsterilized whole peanuts.
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  • 83
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    Biotechnology and Bioengineering 5 (1963), S. 221-230 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process has been developed giving over-all yields of about 50%: which could be increased to about 80% with higher material costs and processing time. The preferred method of recovering nucleotides from the cytoplasm has been thermal extraction by means of a heat exchanger followed by filtration, protein precipitation, and carbon adsorption. The concentrated eluate from the carbon is absorbed onto Dowex 1 ion-exchange resin, and groups of nucleotides are removed by pH-defined eluents and recovered by a second carbon adsorption. Finally, standard ion-exchange resolutions have been used to recover individual nucleotides. Other biochemicals in the extract have been investigated, and preliminary work has been done on the combination of nucleotide extraction with the recovery of nucleic acids from the residual cells.
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  • 84
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    Biotechnology and Bioengineering 5 (1963), S. 231-242 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the present paper the authors described laboratory fermentors used in the Antibiotics Research Institute since 1958 for research concerning the technology of the biosynthesis of antibiotics and amino acids and other aerobic fermentations. The lids of the fermentors are made from stainless steel, the vessels are made from glass. The fermentors are placed, always four each, in eight water baths with automatic temperature regulation within a range of ±0.5°C. Details regarding construction, auxiliary equipment, and working procedures used for the sterilization of fermentors and media, inoculation, injection of additives, and defoaming are given. The set-up for one-stage and multi-stage, batch and continuous cultivation are discussed.
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  • 85
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    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 86
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    Biotechnology and Bioengineering 5 (1963), S. 131-146 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A sensitive cup plate assay method for lysine on large plates using S. faecalis ATCC 6057 as the test organism is described in detail. Well-defined zones of dense growth were obtained in a rectilinear dose - response range from 5 to 3000 μg. of L-lysine/ml. Comparative assays on twelve different samples were carried out using the turbidimetric tube assay method with Leuc. mesenteroides ATCC 8042 and the cup plate method with S. faecalis ATCC 6057. Good agreement between the two assay methods was obtained for five samples. For the remaining seven samples the tube assay values were appreciably higher than those obtained by the plate method. This discrepancy is discussed. Pure lysine added to various sample extracts could be quantitatively recovered. The specificity of the lysine requirement of S. faecalis ATCC 6057 in the plate assay was also investigated. Under certain experimental conditions, a weak and variable response was obtained with D-lysine and the polyamines cadaverine, spermine, and spermidine. There was generally no difficulty in distinguishing the D-lysine and polyamine growth zones from the L-lysine growth zones. This growth effect of D-lysine and polyamines could be diminished and even completely abolished by increasing the DL-hydroxylysine hydrochloride level in the plate medium. The lysine peptides glycyl-L-lysine and L-lysylglycine gave excellent growth zones of the same appearance as those produced by L-lysine. The activity of these two peptides in cup tests were on a molar basis about 70% of that of L-lysine.
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  • 87
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    Biotechnology and Bioengineering 5 (1963), S. 9-15 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method by which a paramagnetic oxygen analyzer is used to determine oxygen consumption and carbon dioxide production during aerobic fermentation of Torulopsis utilis is described. The volume of carbon dioxide produced is not necessarily related to the volume of oxygen consumed by the microorganisms. For this reason, all carbon dioxide is removed from the effluent gas before it enters the analyzer when determining oxygen consumption. A method for calibrating the analyzer to correct for this removal is outlined. When determining carbon dioxide, effluent gas bypasses the carbon dioxide absorption tower before entering the analyzer. The carbon dioxide which it contains dilutes the oxygen remaining in the gas. The magnitude of this dilution can be used to calculate the percentage of carbon dioxide produced in terms of the air flow to the fermentor. Equipment required to alternately measure and record oxygen consumption and carbon dioxide production is described.
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  • 88
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    Biotechnology and Bioengineering 5 (1963), S. 21-25 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cobamide production by Butyribacterium rettgeri Barker and Haas (ATCC 10825) was studied on a laboratory scale. Yields of the order of 5 μg./ml. were obtained when this anaerobe was grown for 6 days at 37°C. in a medium containing glucose, cornsteep liquor, cobalt nitrate, calcium carbonate, and sufficient ammonium hydroxide to keep the pH between 6.0 and 7.2. All of the vitamin was found associated with the bacterial cells, and examination of extracts of these cells showed the presence of 5,6-dimethylbenzimidazolylcobamide coenzyme in large quantities. Supplementation of the fermentation medium with a number of benzimidazoles resulted in formation of traces of “new” cobamides with these bases in the nucleotide protion of the cobamide. Supplementation with a number of purines did not result in incorporation of these moieties in the cobamide portion of the molecule.
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  • 89
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    Biotechnology and Bioengineering 5 (1963), S. 49-52 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An investigation was made of the stability of product limited continuous cultures of Saccharomyces carlsbergensis and Pseudomonas ovalis. No oscillations were found in pH or glucose concentration during the steady state, and no overshoot resulted from changing from one steady state to another.
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  • 90
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    Biotechnology and Bioengineering 5 (1963), S. 41-48 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacillus subtilis NRRL B-2612, isolated at this laboratory, was used to produce polyglutamic acid from devitalized wheat gluten. Conditions for production of the polypeptide were investigated. When NRRL B-2612 was grown on wheat gluten in shaken flasks with phsophate buffer and salts at pH 6.5, yields of polymer reflected a 25-40% incorporation of glutamic acid into polyglutamic acid. Repeated alcoholic precipitation yielded peptides containing between 45 and 90% D-glutamic acid. Physical and chemical properties of a typical purified polyglutamic acid indicated that the product was a gamma-linked polypeptide composed of 75% D-glutamic acid and 25% L-glutamic acid with a molecular weight of approximately 200,000.
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  • 91
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    Biotechnology and Bioengineering 5 (1963), S. 109-121 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbiological production of beta-carotene previously investigated in shakenflask culture has been scaled-up to 20-liter fermentors containing an operating volume of 10-liters. Yields obtained in shaken flasks, approximately 100 mg./100 ml. have been reproduced with a fermentation time of 72 hr. Factors affecting the fermentation: inoculum, mash ingredients, and environmental conditions, were investigated. Typical data are presented. A preliminary cost analysis indicates a “cost to make” of $31.35/kg. of carotene contained in the dried fermentation solids.
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  • 92
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    Biotechnology and Bioengineering 5 (1963), S. 167-184 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Shock pressure waves with a frequency of 50/sec. and a maximal pressure of 60-70 kg./cm.2 greatly reduced the viability of E. coli. A similar effect was obtained also on staphylococci, enterococci, Salmonella Zanzibar, and coliphages T2. On the contrary no effect on spores of B. subtilis was found. The effect was practically independent of the maximal pressure within the range from 30-80 kg./cm.2, the highest pressure tested. The effect decreased with increasing cell density of the bacterial suspension. Bacteria surviving shock treatment died considerably more rapidly when stored at 4°C. than bacteria treated in the same way except for exposure to pressure waves. Morphological examinations with the aid of ordinary light microscopy as well as electron microscopy suggest that the effect was due to mechanical action on the bacterial cell walls.
    Additional Material: 10 Ill.
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  • 93
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 94
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 287-307 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sphaerotilus is usually present in biological waste treatment processes and is considered to be a nuisance organism because it may cause bulking of activated sludge. However, it may play an important role in the decomposition of putrescible organic matter in properly operated biological waste treatment processes. The research described herein is a study of the variety of types of organic compounds which may be decomposed by Sphaerotilus and the effect of some environmental factors upon the rate of decomposition. Although there are some types of compounds which cannot be decomposed by Sphaerotilus, it is shown that this organism is able to assimilate and oxidize a large variety of organic compounds and that it is tolerant of an extremely wide range of environmental conditions. The role of Sphaerotilus in biological waste treatment is discussed in the light of the data presented.
    Additional Material: 9 Ill.
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  • 95
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 355-365 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It has been found that a bacterium (isolant C12B) grew poorly on 6 and 8 carbon alcohols, well on 10 and 12 carbon alcohols, and slowly but well on the 14 to 18 carbon compounds. Unexpectedly, resting cells oxidized the 6 and 8 carbon compounds most readily and cell-free extracts oxidized 6, 8, and 10 carbon alcohols. Extracts from cells adapted to sodium lauryl sulfate oxidized the 12 carbon alcohol as well, if small concentrations of substrate were used. The bacterium grew on benzene sulfonate and adapted resting cells oxidized that compound. Pure culture degradation studies revealed that isolant C12B used only 11% of tetrapropylene ABS supplied as a sole carbon source, whereas 66% of sodium lauryl sulfate was degraded. Intermediate quantities of kerosene-based alkyl benzene sulfonate and a tallow-derived detergent were utilized. Extracts of another bacterium, TEG-5, grown on tetraethylene glycol, contained enzymes which oxidized polyethylene glycols up to an average molecular weight of 600, but not any larger polymers. This oxidation was visualized by coupling to reduction of ferricyanide.
    Additional Material: 9 Ill.
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  • 96
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 17-20 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The applicability of physicochemical adsorption laws to the sorption of methylene blue by dead Penicillium chrysogenum was studied. The specific area (area/mass) of the mold was measured.
    Additional Material: 2 Ill.
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  • 97
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 147-165 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of factors was found to affect the cup plate assay for lysine with Streptococcus faecalis ATCC 6057. Hydroxylysine, aspartic acid, and Tween 80 were found to be among the essential components of the assay medium. Removal of any of these three components produced diffuse growth zones or otherwise unsatisfactory plates. The inclusion of asparagine and additional dipotassium phosphate to the medium was found to be advantageous but not an absolute requirement. It was important not to autoclave the hydroxylysine and the additional dipotassium phosphate together with the rest of the assay medium, but to add these components to the sterilized medium immediately before pouring the plates. With certain concentrations of hydroxylysine in the medium, the addition of L-glutamine was found to potentiate the effect of hydroxylysine and thereby improve the lysine plates. The effect of graded amounts of the above mentioned medium components and of the pH of the assay medium was also studied. Increasing the sterilization time of the medium from 5 to 10 min. at 120°C. improved the readability of the lysine plates and led to more easily reproducible results. Factors influencing the preparation of the inoculum for the lysine plates were also studied: the age of the stab culture, the amount of inoculum used per plate, and the influence of the nature of the suspension medium used for washing the inoculum.
    Additional Material: 1 Ill.
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  • 98
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 193-199 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ferrocyanide-treated beet molasses was fermented in fully baffled mechanically agitated, 2.5-liter, tank-type fermenters using pellets of Aspergillus niger grown in shake flasks as an inoculum. The object of the study was to adapt the fermentation method previously developed in an unstirred tower-type fermenter to a conventional tank-type fermenter. Under selected conditions of stirring (agitator speed 400-700 rpm) and sparging (medium porosity sintered disk), high yields of citric acid (8.6% citric acid, 70% conversion of available sugar) were obtained in 140 hr. of fermentation. There was little relation between citric acid yield and aeration conditions in the fermenter as judged by mash viscosity measurements and sulfite oxidation studies.
    Additional Material: 3 Ill.
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  • 99
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 53-58 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design of a cabinet for the aseptic manipulation of material is described. The interior surfaces of the cabinet are sterilized by swabbing with a germicide. Germ-free conditions are maintained during operation by means of a positive pressure of sterile air. A fan and filter unit attached to the cabinet is used to blow sterile air into the cabinet in sufficient quantity so as to flow out of the hand ports at a velocity of 50 ft./min. In the design, careful consideration was given to the ease of manipulations in the cabinet.
    Additional Material: 1 Ill.
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  • 100
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Removal of the pungent factor, allyl isothiocyanate, will partly determine whether mustard seed can become a commerical source for oil and meal in the United States. In processing studies at the Northern Laboratory, the mustard glucoside was converted enzymatically and the pungent oil was removed. This process has now been extended to pilot-plant scale by using filtration-extraction equipment at the Southern Laboratory. After desolventization and further steam stripping, the extracted meal had a residual content of 2.9% crude fat and 0.004% allyl isothiocyanate.
    Additional Material: 5 Ill.
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