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  • 2000-2004  (16)
  • 1955-1959  (47)
  • 1920-1924
  • Agrobacterium
  • Biochemistry and Biotechnology
  • genetic engineering
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Years
Year
  • 1
    ISSN: 1432-203X
    Keywords: Key words Chickpea ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 mg/l phosphinothricin, depending on the construct used for transformation. Expression of the chimeric GUS gene was confirmed by histochemical localization of GUS activity in regenerated shoots. Resistant shoots were grafted onto 5-day-old dark-grown seedlings, and mature plants could be recovered. T-DNA integration was confirmed by Southern analysis by random selection of putative transformants. The analysis of 4 plantlets of the T1 progeny revealed that none of them was GUS-positive, whereas the presence of the nptII gene could be detected by polymerase chain reaction.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Key words Poplar ; Populus ; Aspen ; Cottonwood ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Keywords: Key words Allium cepa ; Transformation ; Regeneration ; gfp gene ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. The method takes between 3–5 months from explant to primary regenerant entering the glasshouse. Multiple-shoot formation from primary transgenic material made possible the clonal multiplication of transformants. The binary vector used carried the nptII antibiotic resistance gene and the m-gfp5-ER reporter gene. Transgenic cultures were initially screened for their ability to fluoresce and to grow in the presence of geneticin (5–25 mg/l). The transgenic nature of individual plants was confirmed by Southern blot analysis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    International journal of technology and design education 10 (2000), S. 239-254 
    ISSN: 1573-1804
    Keywords: contexts ; critical reflection ; environment ; ethics ; genetic engineering ; impacts ; values
    Source: Springer Online Journal Archives 1860-2000
    Topics: Art History , Education , Technology
    Notes: Abstract Design and technology education aims to prepare young people for living in a rapidly changing technological society which will involve them in making many value judgements, some with complex ethical dimensions. Key aspects of the ethical judgements in relation to genetic engineering are examined: the hidden assumptions, the inevitable unpredictability when dealing with living processes highly interactive with the surroundings, the commercial and political pressures, and the underlying `world-views' and values. It is argued that responsible judgements therefore require wide consultation, sensitivity to social, cultural and moral issues, acknowledgement of the political and economic context, and above all, critical reflection on the beliefs and commitments that are shaping the vision and the drive. Teaching and learning strategies are needed that highlight the social and environmental context of technological activity, that encourage pupils to consider what determines the quality of their own lives and those of others, and that stimulates reflection on the values and beliefs which influence the priorities when value judgements are being made.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1572-9788
    Keywords: Agrobacterium ; inheritance analysis ; Lavandula latifolia ; lavender ; organogenesis ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic plants of the aromatic shrub Lavandula latifolia (Lamiaceae) were produced using Agrobacterium tumefaciens-mediated gene transfer. Leaf and hypocotyl explants from 35–40-day old lavender seedlings were inoculated with the EHA105 strain carrying the nptII gene, as selectable marker, and the reporter gusA gene with an intron. Some of the factors influencing T-DNA transfer to L. latifolia explants were assessed. Optimal transformation rates (6.0 ± 1.6% in three different experiments) were obtained when leaf explants precultured for 1 day on regeneration medium were subcultured on selection medium after a 24 h co-cultivation with Agrobacterium. Evidence for stable integration was obtained by GUS assay, PCR and Southern hybridisation. More than 250 transgenic plants were obtained from 37 independent transformation events. Twenty-four transgenic plants from 7 of those events were successfully established in soil. β-glucuronidase activity and kanamycin resistance assays in greenhouse-grown plants from two independent transgenic lines confirmed the stable expression of both gusA and nptII genes two years after the initial transformation. Evidence from PCR data, GUS assays and regeneration in the presence of kanamycin demonstrated a 1:15 Mendelian segregation of both transgenes among seedlings of the T1 progeny of two plants from one transgenic L. latifolia line.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular breeding 6 (2000), S. 185-194 
    ISSN: 1572-9788
    Keywords: Agrobacterium ; transformation ; pea ; Pisum sativum L. ; PCR analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Factors influencing the efficiency of Agrobacterium-mediated transformation of pea were tested using highly efficient, direct regeneration system. The virulence of three Agrobacterium strains (octopine LBA 4404, nopaline C58C1 and succinamopine, hypervirulent EHA 105) clearly varied giving 1 transgenic plant per 100 explants for LBA 4404, 2.2 for C58C1 and 8.2 for EHA 105. To test the efficacy of selection agents we used the hypervirulent EHA 105 strain carrying pGPTV binary vector with one of four different selection genes: nptII, hpt, dhfr or bar. The mean number of transgenic, kanamycin-resistant plants for two cultivars tested was 4.2 per 100 explants and was slightly higher than the number of phosphinothricin-resistant plants (3.6 plants per 100 explants). The proportion of transgenics among kanamycin-selected plants was also higher than among phosphinothricin-resistant plants (35% and 28% respectively). There was no regeneration on hygromycin or methotrexate media (transformation with hpt and dhfr genes). Acetosyringone had no apparent influence on efficiency of transformation with hypervirulent EHA 105 strain, however it did affect the rate of transformation when moderately virulent C58C1 was used. Recovery of transgenic plants was enhanced after application of 5-azacytidine. The presence of integrated T-DNA was checked by PCR and confirmed by Southern hybridization. T-DNA was stably transmitted to the next generation.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5028
    Keywords: Agrobacterium ; binary vectors ; plant transformation ; reporter genes ; selectable marker genes ; Ti vector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids. Its size and copy number in Escherichia coli offers increased efficiencies in routine in vitro recombination procedures. pGreen can replicate in Agrobacterium only if another plasmid, pSoup, is co-resident in the same strain. pSoup provides replication functions in trans for pGreen. The removal of RepA and Mob functions has enabled the size of pGreen to be kept to a minimum. Versions of pGreen have been used to transform several plant species with the same efficiencies as other binary Ti vectors. Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 62 (2000), S. 141-151 
    ISSN: 1573-5044
    Keywords: Agrobacterium ; forage quality ; KDEL ; recombinant proteins ; sulphur-rich amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Accumulation of zeins, the endosperm storage proteins of maize, in a heterologous plant expression system was attempted. Plants of Nicotiana tabacum and Lotus corniculatus were transformed by Agrobacterium with binary vectors harbouring genes that code for γ-zein and β-zein, two zeins rich in sulphur amino acids. Adding the ER retention signal KDEL to the C-terminal domain modified the zein polypeptides. Significant levels of γ-zein:KDEL and β-zein:KDEL were detected in primary transformants of tobacco. Moreover, the two zeins colocalized in leaf protein bodies of γ-/β-zein:KDEL plants derived from a cross between two primary transformants. Coexpression of γ-zein:KDEL and β-zein:KDEL could be a useful strategy to obtain genotypes of forage legumes which are able to accumulate sulphur amino acids to high levels. As a first step, L. corniculatus plants expressing γ-zein:KDEL in the leaves were obtained.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-6776
    Keywords: Escherichia coli ; genetic engineering ; metal-binding ; OmpC ; protein engineering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The outer membrane protein, OmpC, from Escherichia coli was used to display metal-binding poly-histidine peptides on the surface of this bacterium. SDS-PAGE analysis of outer membrane protein preparations confirmed the expression of the metal-binding epitopes inserted in position 162 of the mature OmpC protein. Display of these epitopes was confirmed by epifluorescence microscopy of cells bound to Ni2+-NTA-agarose beads and metal adsorption experiments. The cells harboring one or two copies of the metal binding epitope were able to adsorb 3 to 6 times more Zn2+ (13.8 μmol g−1 cell), Fe3+ (35.3 μmol g−1 cell), and Ni2+ (9.9 μmol g−1 cell) metallic ions than control cells expressing the wild-type OmpC.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-6776
    Keywords: bioremediation ; genetic engineering ; heavy metal ; hydrogen sulfide ; thiosulfate reductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The thiosulfate reductase gene (phsABC) from Salmonella typhimuriumwas expressed in Escherichia coliin order to produce sulfide from inorganic thiosulfate and precipitate metals as metal sulfide complexes. The sulfide-engineered strain removed significant amounts of heavy metals from the medium within 24 h: 99% of zinc up to 500 μM, 99% of lead up to 200 μM, 99% of 100 μM and 91% of 200 μM cadmium. In a mixture of 100 μM each of cadmium, lead, and zinc, the strain removed 99% of the total metals from solution within 10 h. Cadmium was removed first, lead second, and zinc last. These results have important implications for removal of metals from wastewater contaminated with several metals.
    Type of Medium: Electronic Resource
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 12 (2000), S. 319-330 
    ISSN: 1573-322X
    Keywords: biotechnology ; consumer sovereignty ; genetic engineering ; informed consent ; product labeling ; risk communication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract Recently, both consumers and producers ofbiotechnology products have insisted thatcommunication between the two be improved. The formerdemand more democratic participation in the riskassessment process of biotechnology products. Thelatter seek to correct misinformation regardingalleged risks from these products. One way to resolvethese concerns, I argue, is through the use ofbiotechnology labels. Such labeling fosters consumerautonomy and moves toward more participatory decisionmaking, in addition to ensuring that informed consentfrom consumers is maintained. Furthermore, althoughvoluntary biotech-free labeling in lieu of biotechlabels may uphold consumer sovereignty, the latterremains a more effective strategy for achievingethical communication between consumers and producersof biotechnology products.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 12 (2000), S. 279-303 
    ISSN: 1573-322X
    Keywords: environment ; genetic engineering ; biotechnology ; pesticides ; agriculture ; pest control ; risks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract Despite the application of 2.5 million tons ofpesticides worldwide, more than 40% of all potentialfood production is lost to insect, weed, and plantpathogen pests prior to harvest. After harvest, anadditional 20% of food is lost to another group ofpests. The use of pesticides for pest control resultsin an estimated 26 million human poisonings, with220,000 fatalities, annually worldwide. In the UnitedStates, the environmental and public health costs forthe recommended use of pesticides total approximately$9 billion/yr. Thus, there is a need for alternativenon-chemical pest controls, and genetic engineering(biotechnology) might help with this need. Diseaseand insect pest resistance to various pests has beenslowly bred into crops for the past 12,000 years;current techniques in biotechnology now offeropportunities to further and more rapidly improve thenon-chemical control of disease and insect pests ofcrops. However, relying on a single factor, like theBacillus thuringiensis toxin that has beeninserted into corn and a few other crops for insectcontrol, leads to various environmental problems,including insect resistance and, in some cases, athreat to beneficial biological control insects andendangered insect species. A major environmental andeconomic cost associated with genetic engineeringapplications in agriculture relates to the use ofherbicide resistant crops (HRC). In general, HRCtechnology results in increased herbicide use but noincrease in crop yields. The heavy use of herbicidesin HRC technology pollutes the environment and canlead to weed control costs for farmers that may be2-fold greater than standard weed control costs. Therefore, pest control with both pesticides andbiotechnology can be improved for effective, safe,economical pest control.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 13 (2000), S. 313-327 
    ISSN: 1573-322X
    Keywords: Agri-biotech companies ; agriculture ; biotechnology ; existing technologies ; farmers ; farm crisis ; genetic engineering ; hunger ; poverty ; productivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The use of genetic engineering inagriculture has been the source of much debate. Todate, arguments have focused most strongly on thepotential human health risks, the flow of geneticmaterial to related species, and ecologicalconsequences. Little attention appears to have beengiven to a more fundamental concern, namely, who willbe the beneficiaries of this technology? Given the prevalence of chronic hunger and thestark economics of farming, it is arguable thatfarmers and the hungry should be the mainbeneficiaries of agricultural research. However, theapplication of genetic engineering appears unlikely tobenefit either of these two groups. This technology islargely controlled by the private sector, and itscontinued development hinges on its profitability.Thus, the only likely beneficiaries of the applicationof genetic engineering in agriculture are companieswith the capacity to use it.
    Type of Medium: Electronic Resource
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 13 (2000), S. 43-51 
    ISSN: 1573-322X
    Keywords: genetic engineering ; inherent value ; moral obligation ; Swiss constitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The Swiss expert report suggests thatthe inherent dignity of a living being be identifiedwith its inherent value. But the phrase ``inherentvalue of a living being'' seems to connote two conceptsof inherent value. One has a morally obligatingcharacter but is counterintuitive because of itsegalitarianism. The other is one of non-moral value.It is more compatible with considered intuitions butinsufficient for substantiating the expert report'sclaim that human beings have moral duties towardsanimals and plants. The paper discusses theseconcepts. Consideration is then given to the problemof how discursive support can be generated for theexpert report's claim that human beings have the moralduty to abstain from impairing those functions andabilities of a non‐uman being that members of itsspecies as a rule can practice.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 13 (2000), S. 29-42 
    ISSN: 1573-322X
    Keywords: dignity of creatures ; genetic engineering ; human dignity ; inherent value ; Swiss Constitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract In their report for the Swiss government onthe notion of the dignity of creatures, PhilippBalzer, Klaus-Peter Rippe, and Peter Schaber analyzethe relationship between human dignity and the dignityof creatures, taking them as two categoricallydifferent concepts. Human dignity is defined as the``moral right not to be humiliated,'' whereas thedignity of creatures is taken to be ``the inherentvalue of non‐human living beings.'' To my mind there isno need to draw a categorical distinction between thetwo concepts. Both notions could be brought togetherunder an all-encompassing concept of the inherentvalue of living beings, humans and non-humans alike,a concept one could name ``the dignity of livingbeings.'' Indeed, this very notion underlies theposition taken in the report, although this is notmade explicit by the authors themselves. As the aim of the paper is only to clarify theconcepts used, I do not go beyond this ``internal''critique of their position, i.e., I don't assess howthe claims articulated via these concepts – theclaim that humans and/or creatures have an inherentvalue consisting in a supposed intrinsic good – areto be justified, although I myself would be ratherskeptical that this might be successfully done.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Journal of agricultural and environmental ethics 13 (2000), S. 7-27 
    ISSN: 1573-322X
    Keywords: dignity ; Swiss Constitution ; nonhuman inherent value ; genetic engineering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The 1992 incorporation of an article by referendum in the SwissConstitution mandating that the federal government issue regulations onthe use of genetic material that take into account the dignity ofnonhuman organism raises philosophical questions about how we shouldunderstand what is meant by ``the dignity of nonhuman animals,'' andabout what sort of moral demands arise from recognizing this dignitywith respect to their genetic engineering. The first step in determiningwhat is meant is to clarify the difference between dignity when appliedto humans and when applied to nonhumans. Several conceptions of humandignity should be rejected in favor of a fourth conception: the rightnot to be degraded. This right implies that those who have it have thecognitive capacities that are prerequisite for self-respect. In the caseof nonhuman organisms that lack this capacity, respecting their dignityrequires the recognition that their inherent value, which is tied totheir abilities to pursue their own good, be respected. This value isnot absolute, as it is in the case of humans, so it does not prohibitbreeding manipulations that make organisms more useful to humans. But itdoes restrict morally how sentient animals can be used. In regard togenetic engineering, this conception requires that animals be allowedthe uninhibited development of species specific functions, a positionshared by Holland and Attfield, as opposed to the Original Purposeconception proposed by Fox and the Integrity of the Genetic Make-upposition proposed by Rolston. The inherent value conception of dignity,as here defended, is what is meant in the Swiss Constitution article.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 1-11 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of pH and concentration on foam separation of the protein bovine serum albumin (BSA) from solution has been studied. All results agree, at least qualitatively, with theory. BSA maximum enrichments were observed at the isoelectric pH and enrichment ratio was found to increase with decreasing protein concentrations.
    Additional Material: 5 Ill.
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  • 18
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 13-25 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Most of the free fluid can generally be expressed from fibrous pulps in a few seconds at 50 lb/in2 if the layer of pulp is kept thinner than 1 in. after it has been pressed, and if the fluid is allowed to run away freely. The need for this time and the frictional properties of many pulps are serious obstacles to the design of any strictly continuous press. Roller presses do not allow enough time, screw expellers create too much friction, continuous centrifuges and some other arrangements are intricate and vulnerable.An intermittent but automatic press which avoids these defects is described. In this the material on a perforated conveyor is pressed by a ram and then moved forward as the ram lifts. One such machine has already been made. An outline is given of the principles on which an improved design will depend.
    Additional Material: 10 Ill.
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  • 19
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 27-36 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Equipment has been developed in which fermentation processes for fastidious, pathogenic micro-organisms can be operated on a pilot-plant scale. Experience has shown the need for the development of concepts, techniques, and equipment meeting more stringent requirements than those ordinarily encountered in the fermentation industry. A system has been developed for the preparation of pure cultures which may be used either for the development of processes or in the preparation of substantial quantities of pure cultures. The equipment includes specially designed valves, fermentors, piping arrangement, and requires special methods of operation in order to maintain pure cultures during growth of the organism. The number of cultures of pathogenic, fastidious micro-organisms contaminated in the systems commonly used in industry is materially greater than the number of contaminated cultures produced in the system described.
    Additional Material: 6 Ill.
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  • 20
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A strain of C. purpurea has been isolated which will produce L-tryptophan from indole in submerged culture.The L-tryptophan produced is extracellular and in a free form.Levels of up to 1·5 mg/ml of L-tryptophan have been obtained.
    Additional Material: 4 Ill.
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  • 21
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 37-47 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The results of a study on the surface electric charge carried by fresh-water planktonic algae are used to provide a theory for the flocculation of algae with chemical coagulants. Where it is desirable to remove algae from water, for example from lakes or reservoirs for water supply, or from sewage oxidation ponds, the theory indicates certain practices are desirable to obtain the best removal. It is planned to extend the theory and experimental work to cover the removal of untreated algae from water by filtration through porous granular media, believed to be an adsorptive effect. Separation of different species of algae employing their electrokinetic properties is also described.
    Additional Material: 5 Ill.
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  • 22
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 59-76 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An apparatus is described in which large numbers of algal cultures can be maintained under the following conditions: (1) Constant temperature within the range 5 to 70°C, four different temperatures being maintained simultaneously; (2) uniform illumination at intensities up to 20,000 metre-candles; (3) continuous shaking at variable speed; and (4) aeration under aseptic conditions with mixtures of up to four different gases in any desired proportions.Among other features, thermoregulator heads, flow-meters and heaters for sterile filters, of improved design are described.To illustrate the use of the apparatus, data showing the effects of light intensity and temperature on the growth of Monodus subterraneus are presented.
    Additional Material: 8 Ill.
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  • 23
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 99-113 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nature of the problem of mould growth on baked products is discussed. Existing information on the reduction of this source of wastage by chemical and irradiation techniques is briefly reviewed, and new experimental evidence is presented relating to the use of these procedures. The effectiveness of ionizing radiations in preventing mould growth is indicated but there are many problems to be considered before it can be regarded as a satisfactorily established technique.
    Additional Material: 4 Tab.
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  • 24
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 25
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 77-98 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fractions of markedly different protein contents may be obtained by air-classifying flour (in the sub-sieve range) at cut sizes of about 17 and 35 μ respectively. Of these fractions, the fine, 1 (of high protein content), contains fragments of interstitial endosperm protein and small starch granules; the intermediate, 2 (low protein), mainly large starch granules; the coarse, 3, fragments of endosperm cells. The yields and protein contents of the fractions vary with different wheats; the yields of 1 and 2 may be greatly increased through suitably grinding the flour after milling.In general, 1 may be used for raising the protein content of flours deficient in protein to a level suitable for bread-making. The amount of 1 required depends on its protein contents and on whether it came from a hard or soft wheat, the hard wheat fraction having better bread-making qualities. The resulting variation in the proportion of 1 required in the blend causes variation in the proportion of other particulate materials introduced with 1, in which they have become concentrated during the air classification. These include diastatically active agents and finely divided discolouring matter; the level of the former reached in the blend greatly affects its bread-making value. Pre-grinding of the flour may be helpful in this respect.When chlorinated, 2 is potentially useful for making light-structured cakes, but hard English wheats have mainly given less satisfactory results than soft. Under certain conditions, 3, with or without 2, may be better for biscuit-making than the parent flour.
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  • 26
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 129-141 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Applying radio-isotope 32P, the distribution of bacterial cells retained within glass-fibre air sterilization filters was measured. In particular, the time-dependence of the radial as well as the longitudinal distribution was studied. The collection efficiency of glass-fibre was obtained and the experimental result was compared with a theoretical value based upon the sum of interception, inertia and diffusion. Discrepancies could not be accounted for and are similar to those in the literature. An equation was proposed for the estimation of air filter life. This was not completely verified but leads to some suggestive results.
    Additional Material: 6 Ill.
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  • 27
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 207-215 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The mathematical relationship between the throughput of a batch centrifuge and its mechanical characteristics has been applied to the scale-up of one of the steps in virus processing for possible application to the production of vaccines and similar antigens. Slurries that contained a test virus were purified in a laboratory centrifuge. The Q/Σ relationship, developed by Ambler, was used to predict the operating conditions of a larger centrifuge in order to achieve the same clarification of slurry as that produced in the laboratory centrifuge. A correlation between conditions of centrifugation and amount of virus removed from the feed to the centrifuge shows that there is a well-defined Q/Σ value at which significant concentrations of the test virus are sedimented.
    Additional Material: 3 Ill.
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  • 28
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 239-242 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
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  • 29
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 243-243 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 30
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 229-238 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An account is given of the construction and operation of a fully automatic apparatus in which the material is kept under agitation in a closed container alternately surrounded by hot and cold alcohol supplied from separate baths. The apparatus has the following applications in microbiology and cell physiology: (1)Extraction of micro-organisms and cell tissues by repeated freezing (F) and thawing (T) at variable temperatures and lengths of FT cycle. Extraction can be controlled automatically or manually. As a practical example of the use of the apparatus a description is given of the extraction of living virulent S. typhi bacteria.(2)Studies of the reactions of living micro-organisms and tissues under varying physical conditions (e.g. high- and low-temperature resistance experiments, osmotic studies, thermal synchronization of bacterial cultures, etc.).(3)If required, the normal gas mixture in the residual air space can without difficulty be replaced by other gases or mixtures such as CO2 or N2.
    Additional Material: 7 Ill.
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  • 31
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 217-228 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process is described for the extraction of co-enzyme A from dried yeast. It involves cold water extraction, charcoal adsorption followed by pyridine elution, preparation of an acetone power, co-precipitation as the double cuprous complex with glutathione and removal of the glutathione by means of an ion exchange resin.Improvements over previous processes include use of drum dried yeast plus cold extraction resulting in easy filtration and increase of throughput by a factor of three to five, together with the elimination of the ion exchange step formerly thought necessary to remove zine before the co-precipitation stage. Under the best conditions yields represent 30% recovery of the co-enzyme A in the yeast, the product being 90% pure.
    Additional Material: 4 Ill.
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  • 32
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 33
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 244-244 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 34
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 245-259 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design and construction of a highly sensitive katharometer is described with special reference to the application of the instrument for the precise determination of traces of ethylene by gas chromatography. Factors influencing katharometer sensitivity, and the relative advantages and disadvantages of katharometers as compared with other types of detectors, are discussed.
    Additional Material: 9 Ill.
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  • 35
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 273-288 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An electrophoretic method for the separation of acid polyelectrolytes from proteins in cell extracts is described.A description of the apparatus is given and some results of the separation of extracts from Bordetella pertussis are included.
    Additional Material: 11 Ill.
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  • 36
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 289-295 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An ultrasonic generator was tested at 3 frequencies for its effects on Serratia marcescens during fermentation. The generator controlled foam adequately at 26, 29, and 34 kc/sec at medium aeration rates up to 0·6 ft3/min. Final viable counts were in the same range as those for cultures grown using lard oil as a foam control agent. At 34 kc/sec the final viable counts were slightly higher than those of cultures with lard oil.No harmful effects were evident when large volumes of cultures were exposed to ultrasonic energy during growth.
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  • 37
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The feasibility of producing antibiotics by single-stage continuous fermentation was explored by means of a specially designed pilot plant. Both the chloramphenicol and penicillin processes appeared adaptable to such an operation. At dilution rates of 1·0 and 0·5 volume changes per day respectively, yields of from ¼ to ½ of the maxima obtained in batch operation were maintained in the steady-state for more than 2 weeks.
    Additional Material: 10 Ill.
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  • 38
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 393-412 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Kinetic data are needed to develop basic understanding of fermentation processes and to permit rational design of continuous fermentation processes.The kinetics of the fermentation of glucose to lactic acid have been studied at six constant pH levels between 4·5 and 6·0 by measuring the instantaneous rates of bacterial growth and of lactic acid formation throughout each fermentation.It was found that the instantaneous rate of acid formation dP/dt, could be related to the instantaneous rate of bacterial growth dN/dt, and to the bacterial density N, throughout a fermentation at a given pH, by the expression \documentclass{article}\pagestyle{empty}\begin{document}$\frac{{{\rm d}P}}{{{\rm d}t}} = \alpha \frac{{{\rm d}N}}{{{\rm d}t}} + \beta N$\end{document} where the constants α and β are determined by the pH of the fermentation.
    Additional Material: 11 Ill.
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  • 39
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 413-429 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Information on fermentation process kinetics is potentially valuable for the improvement of batch process performance; it is essential for continuous process design. An empirical examination of rate patterns in various fermentations discloses three basic types: (1) ‘growth associated’ products arising directly from the energy metabolism of carbohydrates supplied, (2) indirect products of carbohydrate metabolism and (3) products apparently unrelated to carbohydrate oxidation. Effects of operating variables on the primary kinetic processes, growth, sugar utilization and antibiotic formation, in the penicillin process, illustrate the special nature of this type.
    Additional Material: 6 Ill.
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  • 40
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Steady-state and transient equations are derived for continuous-flow fermentation processes. Analytical as well as graphical methods for predicting from batch data the performance of single- or multi-stage continuous fermentations are developed. The criteria for stability are also examined. Actual experimental measurements of continuous lactic acid fermentations conducted at controlled pH levels agree with the theory developed.
    Additional Material: 4 Ill.
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  • 41
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 379-392 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pilot plant unit for continuous cultivation of hyphaeforming micro-organisms, providing for a uniform flow velocity in fermentations on suspension media with a massive growth of mycelium, is described. For the maintenance of aseptic conditions, over-pressre of air is maintained in the whole equipment. This equipment has been used for studies of the continuous biosynthesis of streptomycin. A three-stage fermentation proved the most advantageous. The first stage serves for multiplication of the inoculum; the second and third for the formation of the antibiotic. The system was maintained for 300-400 h with yields amounting to 2,000-2,500 u. of streptomycin/ml without any signs of contamination or degeneration of the growing culture.
    Additional Material: 8 Ill.
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  • 42
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 43
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 143-162 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An analysis of the organic constituents of whole, domestic sewage was made by analysing four physically separated fractions. Settlement, centrifuging and filtration were used to obtain one liquid and three solid fractions. Concentration of the filtrate was achieved by freeze-drying with only minor changes in composition. The average concentration of organic carbon in fresh, whole domestic sewage was 310 p.p.m., of which about 70 per cent was in suspension. Eight classes of compounds were found, on analysis, to comprise about 75 per cent of the organic carbon in whole sewage and of the soluble organic carbon 80 per cent was distributed between five groups. Using chromatographic methods the concentrations of four volatile acids were determined and the presence of other acids was detected. Similarly, seven of the soluble sugars and eight present in hydrolysed suspended solids have been identified. The probable nature of some of the compounds unaccounted for is discussed.
    Additional Material: 8 Tab.
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  • 44
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 163-172 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of mechanical agitation in aerobic fermentations is felt mainly through increased aeration efficiency. For yeast propagations in which oxygen supply is abundant - and presumably not limiting to growth - a specific, but limited, effect of fluid agitation on growth rate has been demonstrated. The improved growth rate is attributed primarily to better mixing of the entire fluid mixture rather than to improved cell-fluid mass transfer.
    Additional Material: 6 Ill.
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  • 45
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 115-127 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Information on a reliable method for determining the nutritive value of existing and experimental peptones is lacking in the literature. To overcome this handicap, a method for comparative evaluation of microbiological peptones has been developed.The method is based on measuring the growth of eight carefully selected, fastidious, test cultures. The cultures are grown in presence and absence of yeast extract in an inorganic basal medium containing test peptone and a low and high level of glucose.Statistical analysis of replicate peptone evaluations of six peptones, conducted over a period of six months, shows that 95 per cent confidence limits for this method are ± 2·5 to 7·9 per cent of the mean.Four criteria are employed for determining the overall performance of 12 widely used, commercial peptones.The method is very sensitive for detecting differences between batches of commercial peptones. With the aid of this method a new peptone ‘N-Z-Amine YT’ has been developed. Considering performance and relative cost, this peptone is the most economical of the peptones evaluated.
    Additional Material: 2 Ill.
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  • 46
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 173-184 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A study of factors affecting the reproducibility and yields in cultivations of H. pertussis (the whooping cough organism). The search for suitable media led to the development of a new kind of blood medium suitable for pre-cultures. Sterility of the blood additive could be ensured by filtration, and contamination on starting the freeze-dried cells was avoided by the use of a special kind of ampoule.In the course of studies of different cultivation techniques a 6,000 ml ‘pulsaerator’ was found to be ideal for the preparation of ordinary small batches. Inoculation density, aeration and other factors affecting the yield were studied and a culture cycle suitable for preparing 500 l. batches was developed.
    Additional Material: 2 Ill.
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  • 47
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 185-205 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The mathematical theory of the sedimentation type of centrifuge is developed in terms of unhindered settling of a single particle that has reached equilibrium velocity. From this is derived the sigma value for several of the different forms of sedimentation type centrifuge which is the calculated equivalent area of a settling tank theoretically capable of doing the same amount of work in a unit gravitational field. The limitations controlling the accuracy of scale up between centrifuges of similar geometry and between centrifuges of substantially different geometry are discussed.
    Additional Material: 8 Ill.
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  • 48
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 261-271 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: During the biosynthetic production of chlortetracycline on a medium with sucrose, soy meal, corn steep extract, sodium chloride, ammonium sulphate, calcium carbonate and beet molasses, the amount of nucleic acids synthesized by the culture and the maximum respiration of the culture is determined by the amount of inorganic phosphate in the original medium. Chlortetracycline production does not start until all the inorganic phosphate has been consumed. The adverse influence of increased amounts of inorganic phosphate upon production of chlortetracycline is more apparent in flasks on a shaking machine than in agitated and aerated fermentation tanks with higher oxygen transfer numbers.
    Additional Material: 4 Ill.
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  • 49
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 297-302 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple, rugged and compact automatic feed control attachment for the laboratory-size glass circulating evaporator has been designed and constructed. Experience has demonstrated that this apparatus operates satisfactorily with a variety of aqueous biological extracts and solutions having resistivity values of 50,000 ohm-in. or less. The device in adaptable to other evaporators and stills.
    Additional Material: 2 Ill.
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  • 50
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 303-309 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A description of the cultivation of Rhodospirillum rubrum in 1,000 and 3,000 l. fermentors fitted with a special illumination device, and a discussion of the results.
    Additional Material: 1 Ill.
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  • 51
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    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959) 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 52
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 311-324 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Novobiocin fermentations in 20 l. baffled fermentors were studied as a function of the size and speed of the dual four-bladed flat-blade turbines used. Power input and sulphite oxidation rate measurements were made. The courses of pH, sugar utilization, mycelial dry weight, carbon dioxide evolution and antibiotic titer were determined. Optimum antibiotic yields were achieved at a power input of 0·5 h.p./100 gal, equivalent to a sulphite oxidation rate of 110 mmoles O2/l.h, when the impeller diameters were 29 per cent or 39 per cent of the tank diameter. A power input of 0·75 h.p./100 gal, equivalent to a sulphite oxidation rate of 160 mmoles O2/l.h, was required for equivalent results with the impeller diameter 49 per cent of the tank diameter. Some explanations of the lack of equivalent results with the large impeller are discussed.
    Additional Material: 7 Ill.
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  • 53
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 325-334 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Probability theory has been applied to the analysis of bacterial distribution within a fibrous air sterilization filter. For a long filter layer, to which the so-called ‘log-penetration’ law is no longer applicable, the probability theory is considered to be effective in interpreting the distribution data.The effects of the diameter, volume fraction and moisture content of the glass-fibre on the longitudinal distribution of bacterial cells, were studied. The distribution was estimated beyond the experimental data points. This estimation will be of use for predicting the ‘life’ of an air sterilization filter.
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  • 54
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    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 349-357 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The equations of Monod that describe the course of bacterial population growth and substrate concentration during continuous culturing are usually analysed with reference to steady-state conditions. These equations are analysed to give information on the course of bacterial population growth from inoculation to the achievement of a steady-state condition. For common values of certain parameters occurring in Monod's development, growth is well approximated by an exponential law (logarithmic phase) which depends on: (a) the maximum specific growth-rate constant for the organism and medium concerned, and (b) the constant dilution rate.
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  • 55
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of agitation on the rate of acid formation by the microaerophilic organism Lactobacillus delbrueckii was studied. Fermentations were conducted at constant temperature and pH, and with the continuous addition of a nutrilite source. The range of agitator speeds was from 85 to 720 rev/min.The acid formation rate was found to increase with increasing agitator speeds and decrease with decreasing speeds if corn-steep liquor was the nutrilite source. No effect of agitation was observed with yeast extract as the nutrilite source.The data from corn-steep liquor media were found to be consistent with a mass transfer mechanism, but did not warrant a conclusion as to the specific nature of the effect of agitation. It was concluded, however, that the effect of agitation on the acid formation rate was at most very small.
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  • 56
    ISSN: 1573-5060
    Keywords: Agrobacterium ; transformation ; lily ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Lily cv. Harmony was inoculated with several Agrobacterium strains to study its susceptibility to Agrobacterium infection and transformation. Tumorous tissue formation on inoculated stem internodes of sterile-grown plantlets, as well as expression of a β-glucuronidase marker gene interrupted by an intron in cells of inoculated stem nodes, indicate that the monocotyledon Lilium is a host for Agrobacterium.
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  • 57
    ISSN: 1573-5060
    Keywords: Agrobacterium ; Brassica napus ; CaMV 35S promoter ; mas promoter ; gene expression ; risk assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Gene fusions between the β-glucuronidase (GUS) reporter gene and the promoters of the cauliflower mosaic virus 35S RNA transcript (CaMV 35S) and the mannopine synthase (mas) genes were introduced into rapeseed varieties via Agrobacterium-mediated transformation. Fluorometric assay of β-glucuronidase activity indicated different expression patterns for the two promoters. In seedlings, the CaMV 35S promoter had maximum activity in the primary roots, while the mas promoter was most active in the cotyledons. Etiolated seedlings cultured in the dark showed reduced activity of the mas promoter. Before vernalization at the rosette stage, both promoters were more active in older plant parts than in younger ones. At this stage the highest activity was recorded in cotyledons. After the plants had bolted reduced promoter function was detected in the upper parts of the transformed plants. Both promoters were found to be functional in the majority of the studied organs of transgenic rapeseed plants, but the promoter activity varied considerably between the organs at different developmental stages. The ability of pollen to transfer the introduced genes to other varieties and related species (e.g. Brassica napus and Diplotaxus muralis) by cross-pollination was studied in greenhouse experiments, and field trials were carried out to estimate the distance for biologically-relevant gene dispersal. In artificial crossing, the introduced marker gene was transferable into other varieties of Brassica napus. In field trials, at a distance of 1 metre from the source of transgenic plants, the frequency of an outcrossing event was relatively high (10-3). Resistant individuals were found at 16 and 32 metres from the transgenic pollen donors, but the frequency of an outcrossing event dropped to 10-5.
    Type of Medium: Electronic Resource
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  • 58
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Indica-type rice ; genetic engineering ; vitamin A endosperm ; insect resistance ; virus resistance ; fungus resistance ; essential amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Indica-type rice provides the staple food for two billion people in Third World countries. Several problems involved in the stable and sustained production of high quality food cannot be solved by traditional breeding. Methods have been established for gene transfer to Indica rice breeding lines to study possible contributions from genetic engineering. Experiments are in progress on the development of transgenic resistance towards Yellow Stem Borer, resistance towards Rice Tungro Virus, accumulation of provitamin A in the endosperm, increase of essential amino acids in the endosperm such as lysine, cysteine and methionine and resistance towards fungal pests such as Rice Blast and Sheath Blight. Transgenic clones from Indica rice breeding lines have been recovered from several of the approaches mentioned, some of which have been regenerated to plants.
    Type of Medium: Electronic Resource
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  • 59
    ISSN: 1573-5060
    Keywords: genetic engineering ; introgression ; molecular markers ; potatoes ; resistances ; Solanum ; technology mansfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato genetic improvement has been facilitated using new knowledge of potato reproductive biology and new techniques. Many wild diploid species as well as landrace cultivars have been used in breeding at the diploid level, a strategy which is supported by 1) 2n gametes and 2) haploids from tetraploid cultivars. Different categories of wild species which have been under-utilized are now being exploited further in more systematic enhancement programmes using semi-conventional and biotechnological methods. Molecular maps of the potato genome are used actively to achieve marker-assisted introgression and improved selection among the germplasm collections to facilitate the use of valuable wild genetic resources. As an alternative method to incorporate a high level of fesistance, genetic engineering has been employed to facilitate the initial breeding process using various gene constructs for controlling major biotic stresses in the world.
    Type of Medium: Electronic Resource
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  • 60
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 85 (1955), S. 1-12 
    ISSN: 1573-5060
    Keywords: genetic engineering ; gene targets ; mapping ; markers ; transformation ; QTLs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 61
    ISSN: 1573-5060
    Keywords: Linum usitatissimum ; linseed ; mutation breeding ; somaclonal variation ; fatty acids ; genetic engineering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the early 1980s the phenomenon of somaclonal variation induced by cell culture was exploited to produce genetic variation in linseed. The linseed variety Andro, derived from the widely grown Canadian variety McGregor, was selected in saline culture and was released for production in Canada. ‘Andro’ possesses traits very different from its parent, such as increased seedling vigour and tolerance to heat stress. Additional stable somaclonal variation in characters such as yield, days to maturity, seed weight and oil content were subsequently induced in ‘McGregor’. However, despite extensive screening of the somaclonal variants, no significant variation in the fatty acid profile was found. Chemical mutagenesis using ethyl methanesulphonate was, however, succesful in modifying the fatty acid profile of McGregor. Initial screening of M2 seed by the thiobarbituric acid colourimetric procedure was followed by gas chromatography to select half-seeds with atypical fatty acid profiles. Two independent, partially dominant genes were identified that were responsible for reducing the linolenic acid (18 : 3) from 50% to 2% while increasing linoleic acid (18 : 2) to 70%. A single, partially dominant gene, inherited independently of the linolenic acid genes, increased palmitic acid (16 : 0) from 7% to 30% and palmitoleic acid (16 : 1) from trace amounts to 4%. Agrobacterium-mediated transformation of linseed has also been successful. Herbicide tolerance genes for glyphosate, sulfonylurea and phosphinothricin have been incorporated into Canadian varieties. Commercially useful levels of tolerance to sulfonylurea herbicides have been achieved with no adverse agronomic affect. It is expected that a transgenic variety containing this resistance will be registered for commercial production in Canada in 1994. Standard breeding techniques, the application of antisense technology and the overexpression of fatty acid synthesis genes are being used to further modify the fatty acid profile of linseed, as well as for the transfer of abiotic stress-related genes identified in bromegrass.
    Type of Medium: Electronic Resource
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  • 62
    ISSN: 1573-5060
    Keywords: Agrobacterium ; plant regeneration ; potato ; Solanum tuberosum ; tissue culture ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To provide a truly genotype-independent transformation system, it is necessary to be able to transform a wide range of potato genotypes. The ability to regenerate shoots in vitro was determined for 34 potato varieties using tuber disc explants. Following a culture regime used extensively in previous studies with the variety Desiree, half of the varieties could be regenerated from tuber discs and half could not. From a sample of varieties that could be regenerated from tuber discs, all but one variety gave transgenic plants. Twelve varieties were evaluated for the capacity to regenerate shoots from leaf and internode explants excised from in vitro grown plants. All of the varieties tested regenerated adventitious shoots. Leaf and internode explants from 5 varieties were subsequently used for transformation, and transgenic plants were produced from two potato varieties that did not give transgenic plants from tuber disc explants. Some varieties could not be transformed by either method, and will require modification of the in vitro regeneration and transformation system to be successful.
    Type of Medium: Electronic Resource
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  • 63
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 85 (1955), S. 131-134 
    ISSN: 1573-5060
    Keywords: apple ; transformation ; Agrobacterium ; preculture ; azacytidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf explants of apple cvs Gala and Golden Delicious were infected with the Agrobacterium tumefaciens strain AGL0(pMOG410). The effects of a 2 d preculture of the explants before infection and the addition of 5-azacytidine to the selection medium were studied. The percentages of GUS-positive explants after 5 w did not significantly alter due to these treatments. One of the ‘Gala’ shoots, which was removed from a leaf explant cultured for 8 w on selection medium, proved to be GUS-positive and will be analyzed further. In general, however, it should be concluded that regeneration of transgenic shoots directly from leaf tissue was not very effective.
    Type of Medium: Electronic Resource
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