ZIB

1

Electronic Resource

A Modular Vector for Agrobacterium Mediated Transformation of Wheat (1999)

Peters, Noël R. ; Ackerman, Steven ; Davis, Elizabeth A.

Springer

Plant molecular biology reporter 17 (1999), S. 323-331

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ISSN: 1572-9818

Keywords: Agrobacterium ; modular vector ; transformation ; wheat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Wheat (cv Chinese Spring) tissues were transformed using Agrobacterium tumefasciens and a new plasmid modular vector, pMVTBP. We constructed pMVTBP with unique restriction sites connecting (1) the CaMV 35S promoter, (2) a Kozak sequence, (3) the FLAG epitope, (4) the (His)6 epitope, (5) a coding region (for wheat TATA Binding Protein, wTBP) and (6) the CaMV 35S 3′UTR. This vector thus allows easy exchange of different regulatory or coding sequences. Explants of either germinating mature seeds, or immature embryos, were induced to callus for up to two weeks, treated with virulence-induced bacteria for one hour, then regenerated into plantlets. Transient expression of a GUS reporter gene, assayed at about one week, occurred in 10–12% of calluses. Expression of the FLAG-tagged wTBP was also detected, by immunostaining. Stable expression, by selective growth on geneticin, and by GUS expression at about six weeks, occurred in 1–2% of calluses, quite comparable to that achieved by other methods.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007686408369

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2

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Polymorphism in Polyamide of Dytek®-A and Dodecanedioic Acid (1999)

Keating, M. Y. ; Gardner, K. H. ; Ng, H. ; [et al.]

Springer

Journal of thermal analysis and calorimetry 56 (1999), S. 1133-1140

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ISSN: 1572-8943

Keywords: branched diamine ; melting ; polyamides ; polymorphism ; transformation ; WXRD

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract Our X-ray work of Dytek®-A, 2-methyl-pentamethylenediamine, containing polyamides shows polymorphism, whereas the polyamides with linear diamines do not. The polyamide of Dytek®-A and dodecanedioic acid, MPMD-12, is singled out for discussion and compared with the unbranched analogs of polyamides 6,12 and 5,12. Due to the presence of the -CH3 side group in the 2-position of the diamine, the polyamide MPMD-12 exhibits two stable crystal conformations. The new δ polymorph is not seen in linear polyamides 6,12 and 5,12. Studies by DSC polyamide MPMD-12 clearly illustrates at least two crystal forms, γ and δ, coexisting over a wide temperature range, and the isolation of each phase is possible by controlling temperature and time. The DMA modulus in the temperature region between the glass transition (or alpha relaxation) and melting transition shows strong dependence on the thermal history as demonstrated in a study of crystallization kinetics.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1010105028316

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3

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Agrobacterium‐mediated transformation of lavandin (Lavandula x intermedia Emeric ex Loiseleur) (1999)

Dronne, Sandrine ; Moja, Sandrine ; Jullien, Frédéric ; [et al.]

Springer

Transgenic research 8 (1999), S. 335-347

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ISSN: 1573-9368

Keywords: Agrobacterium tumefaciens ; ß‐glucuronidase ; lamiaceae ; lavandin ; neomycin phosphotransferase II ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Lavandin (Lavandula x Emeric ex Loiseleur) is an aromatic plant, the essential oil of which is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. The qualitative or quantitative modification of its terpenes‐containing essential oil by genetic engineering could have important scientific and commercial applications. In this study, we report the first Agrobacterium tumefaciens‐mediated gene transfer into lavandin. The transformation protocol was optimized by lengthening precultivation and cocultivation periods and by testing five different bacterial strains. We obtained transformed callus lines at a frequency of 40–70 with strains AGL1/GI, EHA105/GI and C58/GI. Transgenic shoots were regenerated from these kanamycin resistant calli and rooted on selective medium with 150 mg l-1 kanamycin. The final percentage of transgenic plants obtained varied from 3 to 9, according to the strain used, within 6 months of culture. The presence of the introduced β‐glucuronidase and neomycin phosphotransferase II genes was shown both by PCR and Southern blot analysis. Transgene expression was investigated using histoenzymatic β‐glucuronidase assays, leaf callus assays and RT‐PCR. Results showed that both β‐glucuronidase and neomycin phosphotransferase II genes were expressed at a high level in at least 41 of the transgenic plants regenerated. This efficient transformation strategy could be used to modify some genetic traits of lavandin (flower colour, pathogens resistance) and to study the biosynthesis of the major monoterpene components of its essential oil (linalool, linalyl acetate, camphor and 1,8‐cineole).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008948113305

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4

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Stable Long Term Germ-Line Transmission of Transgene Integration Sites in Mice (1999)

Aigner, Bernhard ; Fleischmann, Michaela ; Müller, Mathias ; [et al.]

Springer

Transgenic research 8 (1999), S. 1-8

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ISSN: 1573-9368

Keywords: gene construct ; gene transfer ; heritability ; marker gene ; pigmentation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Transgenic mice provide a valuable tool in all fields of basic and applied biological and medical research. In this study, we describe the fate of integrated transgenes in the mammalian host genome over a large number of generations. The stability of the germ-line transmission of integrated tyrosinase transgene copies was monitored up to generation F20 in a large number of individuals from seven transgenic mouse lines. Phenotypic and molecular genetic analysis of the offspring both within the different lines and in cross-breeding experiments revealed the high stability of the transgene integration sites in mice. Only very few individuals were affected by a transgene copy loss. These results indicate that, once homozygous transgenic lines are established, breeding programs can be continued to a high number of generations without further stringent molecular genetic analysis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008824028100

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5

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Expression of the Bar Gene Confers Herbicide Resistance in Transgenic Lettuce (1999)

Mohapatra, Umaballava ; McCabe, Matthew S. ; Power, J. Brian ; [et al.]

Springer

Transgenic research 8 (1999), S. 33-44

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ISSN: 1573-9368

Keywords: Lactuca sativa ; Agrobacterium tumefaciens ; bialaphos ; phosphinothricin acetyltransferase ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Resistance to bialaphos, a broad-spectrum herbicide, was introduced into Lactuca sativa cv. Evola by Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strains 0310 and 1310, both carrying the bialaphos resistance (bar) and neomycin phosphotransferase (nptII) genes, were used for transformation. Primary transformants were selected on kanamycin sulphate-supplemented shoot regeneration medium. Integration of both transgenes was confirmed by non-radioactive Southern hybridisation. The hypervirulent plasmid ToK47 in A. tumefaciens strain 1310 generated multiple insertions of T-DNA in some transgenic plants; the absence of pToK47 (strain 0310) resulted in single gene inserts in all plants tested. Resistance to glufosinate ammonium was observed in axenic seedlings grown on medium supplemented with the herbicide at 5 mg l−1 and in glasshouse-grown plants sprayed with the compound at 300 mg l−1. Stable expression of the bar gene was observed in R2 generation plants. The kanamycin resistance of R1 seedlings was observed by germinating seeds on medium supplemented with 200 mg l−1 kanamycin sulphate. The presence of NPTII protein and PAT enzyme activity were demonstrated by ELISA and PAT enzyme assay respectively. Transgenes segregated in a Mendelian fashion in some plant lines in the R1 generation; herbicide resistance also segregated in the expected ratio in the R2 generation in most transgenic lines. This study confirmed that an agronomically important transgene can be integrated and stably expressed over several generations in lettuce.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008891216134

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6

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Gene transfer into canine myoblasts (1999)

Braun, Serge ; Thioudellet, Christine ; Perraud, Frédéric ; [et al.]

Springer

Cytotechnology 30 (1999), S. 181-189

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ISSN: 1573-0778

Keywords: culture ; dog ; Duchenne dystrophy ; gene transfer ; satellite cells

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract We have developed and characterized cultures of healthy and dystrophic canine myoblasts for the evaluation of various gene transfer protocols. The number of desmin-positive myoblasts was elevated (〉〉80%) in cultures of myoblasts obtained from different muscle territories, the diaphragm muscle giving rise to the purest cultures. Myoblasts from dogs turned out to be a very convenient source of well transfectable and transducible cells. Transfection with plasmid DNA allowed efficient transgene expression (50% of β-galactosidase positive cells and about 375 ng luciferase/mg protein after transfection with a calcium phosphate-precipitated plasmid). Infection with high concentrations of adenoviral and retroviral vectors allowed transgene (β-galactosidase or mini-dystrophin) detection in about 75 to 90% of the canine cells. Therefore, primary dog myoblast cultures represent a useful in vitro model for viral and non-viral gene delivery, as well as for functional evaluation and cell grafting with applications in genetic diseases, vaccination or production of circulating therapeutic proteins.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008026913715

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7

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Transformation of pasta wheat (Triticum turgidum L. var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality (1999)

He, G.Y. ; Rooke, L. ; Steele, S. ; [et al.]

Springer

Molecular breeding 5 (1999), S. 377-386

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ISSN: 1572-9788

Keywords: Triticum turgidum L. var. durum ; pasta wheat ; transformation ; seed protein modification ; flour quality improvement

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract Particle bombardment has been used to transform three cultivars (L35, Ofanto, Svevo) and one breeding line (Latino × Lira) of durum wheat (Triticum turgidum L. var. durum). These varieties were co-transformed with plasmids containing selectable and scorable marker genes (bar and uidA) and plasmids containing one of two high-molecular-weight (HMW) glutenin subunit genes (encoding subunits 1Ax1 or 1Dx5). Ten independent transgenic lines were recovered from 1683 bombarded scutella (transformation efficiency thus 0.6%). Five lines expressed either subunit 1Dx5 or 1Ax1 at levels similar to those of endogenous subunits encoded on chromosome 1B. To identify the effects of the transgenes on the functional properties of grain, three lines showing segregation for transgene expression were used to isolate sibling T2 plants which were null or positive for the transgene product. Analysis of these plants using a small-scale mixograph showed that expression of the additional subunits resulted in increased dough strength and stability, demonstrating that transformation can be used to modify the quality of durum wheat for bread and pasta making.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1009681321708

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8

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Transformation of the endospermous legume guar (Cyamopsis tetragonoloba L.) and analysis of transgene transmission (1999)

Joersbo, Morten ; Brunstedt, Janne ; Marcussen, Jan ; [et al.]

Springer

Molecular breeding 5 (1999), S. 521-529

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ISSN: 1572-9788

Keywords: Cyamopsis tetragonoloba ; β-lactamase inhibitor ; sulbactam ; transformation ; transgene stability ; transgenic guar

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract A procedure for transformation of the large-seeded endospermous legume guar (Cyamopsis tetragonoloba L.) and a study on transmission of the transgenes to offspring generations are presented. Using Agrobacterium tumefaciens with a T-DNA construct harbouring a β-glucuronidase gene (uidA) and a neomycin phosphotransferase gene (nptII), maximum transformation frequencies of cotyledonary explants were obtained using 145 mg/l kanamycin sulfate as selective agent. Carbenicillin and cefotaxime, used for the elimination of Agrobacterium after co-culture, displayed considerable toxicity to guar tissues but replacing most of these β-lactams by the non-phytotoxic β-lactamase inhibitor sulbactam as well as addition of thidiazuron and silver thiosulfate increased transformation frequencies up to 10-fold in total. The presence of the transgenes in the primary transformants was demonstrated by genomic DNA analysis of GUS-positive shoots. Chimaeric plants (5–10%) were identified by GUS analysis at the flowering stage and were discarded. Analysis of the R1 offspring from 17 independent transformants showed that in 41% of those, the uidA gene(s) was expressed and stably inherited consistent with Mendelian genetics. This was also found for the R2 and R3 generations of single copy transformants. On the other hand, a large proportion (47%) of the primary transformants gave R1 offspring in which 100% of the plants were GUS-negative. Analysis of these plants by PCR revealed that, at least, most of the transgene sequences were absent, suggesting that they had not been transmitted from the parent transformants. This occurred at similar high frequencies (40–50%) irrespective of the estimated copy number of the transgenes. Thus, major parts of the transgenes, even when present in multiple copies, displayed aberrant transmission, at a high frequency, in the process of going from the primary transformants to the first offspring generation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1009602722423

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9

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Partial male sterility in transgenic tobacco carrying an antisense gene for alternative oxidase under the control of a tapetum-specific promoter (1999)

Kitashiba, Hiroyasu ; Kitazawa, Erina ; Kishitani, Sachie ; [et al.]

Springer

Molecular breeding 5 (1999), S. 209-218

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ISSN: 1572-9788

Keywords: alternative oxidase ; antisense ; male-sterility ; tapetum-specific promoter ; tobacco ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract The alternative oxidase of plant mitochondria is the terminal oxidase of the cyanide-insensitive respiratory pathway and is encoded by a nuclear gene. A 1 kb genomic fragment including exon 3 of the alternative oxidase was amplified by PCR from the genome of Arabidopsis thaliana. This fragment was connected to a tapetum-specific promoter in the antisense orientation and then introduced into tobacco. The pollen viability in three transgenic plants ranged from 2% to 60%. The reduced pollen viability cosegregated with the transgene in a selfed progeny. Immunolocalization of alternative oxidase protein in the immature flower bud section indicated that expression of alternative oxidase protein in tapetum of the transgenic plant was much lower than that of the non-transformant. The histological observation and protein gel-blot analysis showed that the development of pollen grains in the transgenic plant did not progress after the degradation of the tapetum, and the amount of alternative oxidase in pollen grains of the transgenic plant became lower than that of the non-transformant. These results suggested that the alternative oxidase activity in the tapetum has a significant effect on the pollen development.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1009606601521

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10

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Expansive Visibilization of Work: An Activity-Theoretical Perspective (1999)

Engeström, Yrjö

Springer

Computer supported cooperative work 8 (1999), S. 63-93

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ISSN: 1573-7551

Keywords: activity theory ; action ; transformation ; expansive learning ; intervention ; visibilization ; health care ; medical records

Source: Springer Online Journal Archives 1860-2000

Topics: Computer Science

Notes: Abstract Work is commonly made visible along two dimensions: the linear and the socio-spatial. Both are limited to depicting work in terms of relatively discrete actions. Activity theory introduces the crucial distinction between collective activity systems and individual actions. Expansive visibilization of collective activity systems offers a powerful intervention methodology for dealing with major transformations of work. The linear and the socio-spatial dimensions of work actions are seen in the broader perspective of a third, developmental dimension of work activity. Four steps are identified in a cycle of expansive visibilization, combining activity-level visions and action-level concretizations. The cycle is examined in detail as it unfolded in an intervention study at a children's hospital in Finland. It is concluded that expansive visibilization, driven by contradictions and seeking to reconceptualize the object and motive of work, is not a straightforward process which can be neatly controlled from above. Coherent analytical explanation and goal-setting may come only after the creation and practical implementation of innovative solutions.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008648532192

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Adenovirus-mediated Gene Transfer of a Truncated Form of Fibroblast Growth Factor Receptor Inhibits Growth of Glioma Cells both In Vitro and In Vivo (1999)

Saiki, Masaaki ; Mima, Tatsuo ; Takahashi, Jun C. ; [et al.]

Springer

Journal of neuro-oncology 44 (1999), S. 195-203

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ISSN: 1573-7373

Keywords: adenovirus ; dominant negative ; fibroblast growth factor receptor ; gene transfer ; glioma

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Basic fibroblast growth factor (FGF-2) and high affinity FGF receptor (FGFR) have been detected in the nucleus as well as the cytoplasm of many human gliomas, and are known to stimulate cellular proliferation and angiogenesis in the tumors. To investigate the effects of inactivation of FGFR on the growth of malignant gliomas, we constructed a replication-deficient recombinant adenovirus vector encoding a truncated form of chicken FGFR1 (AxCA Δ FR). AxCA Δ FR-infected cells were confirmed to express truncated FGFR protein by immunoblotting and FGF-2-dependent clonogenicity of NIH3T3 cells was suppressed by infection with this virus vector. Then human malignant glioma cell lines U-251MG and T98G, both of which have been reported to express FGF-2 and FGFR, were infected with AxCA Δ FR. These infected cells showed nuclear as well as cytoplasmic expression of a truncated FGFR protein. Proliferation rate and the ability to form colonies in soft agar of the cells infected with this virus vector were significantly suppressed compared with those of uninfected and lacZ-expressing adenovirus-infected cells. Moreover, intratumoral injection of AxCA Δ FR significantly suppressed the subcutaneous tumor growth of the glioma cells in nude mice. We concluded that inactivation of the cytoplasmic and nuclear FGFR using this truncated FGFR-expressing adenovirus vector can inhibit the growth of malignant gliomas both in vitro and in vivo.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006355014351

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12

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Glycotargeting: Influence of the Sugar Moiety on Both the Uptake and the Intracellular Trafficking of Nucleic Acid Carried by Glycosylated Polymers (1999)

Monsigny, Michel ; Midoux, Patrick ; Mayer, Roger ; [et al.]

Springer

Bioscience reports 19 (1999), S. 125-132

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ISSN: 1573-4935

Keywords: Oligonucleotides ; gene transfer ; routing ; membrane lectins ; glycoconjugates

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract Nucleic acids (plasmids as well as oligonucleotides) used to specifically express or modulate the expression of a gene, must reach the cytosol and/or the nucleus. Several systems have been developed to increase their uptake and their efficiency. Glycosylated polylysines have been shown to specifically help nucleic acids to be taken up in cells expressing a given cell surface membrane lectin. However, it appeared that the efficiency of the imported nucleic acid was not directly related to the extent of the uptake. Indeed, some glycosylated polylysines bearing sugar moities which are poor ligands of the cell surface lectins of a given cell were found to be more efficient than those bearing better sugar ligands. The interpretation of this paradoxal result is discussed with regards to the nature of the compartment allowing the nucleic acid to cross the membrane and to be delivered in the cytosol on the one hand, and to the presence of intracellular lectins on the other hand.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1020114611517

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13

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REMI (Restriction Enzyme Mediated Integration) and its Impact on the Isolation of Pathogenicity Genes in Fungi Attacking Plants (1999)

Kahmann, Regine ; Basse, Christoph

Springer

European journal of plant pathology 105 (1999), S. 221-229

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ISSN: 1573-8469

Keywords: mutagenesis ; transformation ; plant disease ; recombination ; plant pathogenic fungi

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract Development of molecular techniques for phytopathogenic fungi aims at the identification of fungal genes whose products are essential for successful infection of the host plant. Initial approaches have relied on isolating candidate genes and generating null-mutations by homologous recombination. Unfortunately, the results of this strategy have not been overly successful. This has led to a search for alternatives which allow an unbiased identification of pathogenicity genes. One method, which has proved successful in several systems, is a tagging mutagenesis procedure termed restriction enzyme mediated integration (REMI). In this mini-review we describe this procedure and review its features and results of its use when applied to the identification of fungal genes required for disease development in planta.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008757414036

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14

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A mini binary vector series for plant transformation (1999)

Xiang, Chengbin ; Han, Peng ; Lutziger, Isabelle ; [et al.]

Springer

Plant molecular biology 40 (1999), S. 711-717

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ISSN: 1573-5028

Keywords: Agrobacterium tumefaciens ; Arabidopsis thaliana ; binary vector ; T-DNA ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A streamlined mini binary vector was constructed that is less than 1/2 the size of the pBIN19 backbone (3.5 kb). This was accomplished by eliminating over 5 kb of non-T-DNA sequences from the pBIN19 vector. The vector still retains all the essential elements required for a binary vector. These include a RK2 replication origin, the nptIII gene conferring kanamycin resistance in bacteria, both the right and left T-DNA borders, and a multiple cloning site (MCS) in between the T-DNA borders to facilitate cloning. Due to the reduced size, more unique restriction sites are available in the MCS, thus allowing more versatile cloning. Since the traF region was not included, it is not possible to mobilize this binary vector into Agrobacterium by triparental mating. This problem can be easily resolved by direct transformation. The mini binary vector has been demonstrated to successfully transform Arabidopsis plants. Based on this mini binary vector, a series of binary vectors were constructed for plant transformation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006201910593

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The S7 ribosomal protein gene is truncated and overlaps a cytochrome c biogenesis gene in pea mitochondria (1999)

Zhuo, Degen ; Thi Nguyen-Lowe, Ha ; Subramanian, Selvi ; [et al.]

Springer

Plant molecular biology 40 (1999), S. 91-97

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ISSN: 1573-5028

Keywords: cytochrome c biogenesis ; gene transfer ; mitochondria ; pea ; ribosomal protein ; soybean

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The pea mitochondrial genome contains a truncated rps7 gene lacking ca. 40 codons at its 5′ terminus. This single-copy sequence is immediately downstream of and slightly overlapping an actively transcribed and edited reading frame of 744 bp (designated ccb248) homologous to the bacterial helC gene which encodes a subunit of the ABC-type heme transporter involved in cytochrome c biogenesis. This region of mitochondrial DNA appears recombinogenic, and the carboxy-termini of helC-type proteins are predicted to vary in sequence and length among plants. Sequences corresponding to the 5′ coding region of rps7 were not detected elsewhere in the pea mitochondrial genome using wheat rps7 probes, and only a very short internal rps7 segment was observed in soybean mitochondrial DNA. The presence of rps7-homologous sequences in the nuclear genomes of pea and soybean is consistent with the recent transfer of a functional mitochondrial rps7 gene to the nucleus in certain plant lineages.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1026499906338

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Mycoplasma-Mediated Uptake of the Exogenous Human BRCA1 Gene by Hatching Blastocysts (1999)

Chan, Philip J. ; Brossfield, Jeralyn E. ; Patton, William C. ; [et al.]

Springer

Journal of assisted reproduction and genetics 16 (1999), S. 546-550

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ISSN: 1573-7330

Keywords: breast cancer ; mycoplasma ; Ureaplasma urealyticum ; foreign DNA ; gene transfer ; transgenic

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Purpose: Biological vectors for cell transfection are mainly viral in origin, with inherent shortcomings. Mycoplasmas are ubiquitous organisms that traverse cells easily. The objective was to determine if Ureaplasma urealyticum (T-mycoplasma) would vector exogenous BRCA1 DNA into blastocysts. Methods: Hatching mouse blastocysts (N = 70) were incubated in the presence of either viable or dead Ureaplasma urealyticum at 37°C for 1 hr. The blastocysts were exposed to human BRCA1 DNA lacking homology in the mouse genome for 2 hr, followed by DNase-I treatment and wash. Polymerase chain reaction and agarose gel electrophoresis analysis of amplified products were performed. Results: The BRCA1 gene was detected in the blastocysts only when viable Ureaplasma was present. PCR analyses of control Ureaplasma and untreated blastocysts were negative. Conclusion: Viable Ureaplasma organisms were shown to mediate the uptake of DNA fragments into blastocysts, resulting in transgenic mouse blastocysts with a normal human BRCA1 exon 11 gene.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1020553322073

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Wnt Signalling in Mammalian Development and Cancer (1999)

Smalley, Matthew J. ; Dale, Trevor C.

Springer

Cancer and metastasis reviews 18 (1999), S. 215-230

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ISSN: 1573-7233

Keywords: transformation ; tumour ; Frizzled ; Dishevelled ; glycogen synthase kinase-3β

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Wnt signalling is involved in a variety of mammalian developmental processes, including cell proliferation, differentiation and epithelial–mesenchymal interactions, through which they contribute to the development of tissues and organs such as the limbs, the brain, the reproductive tract and the kidney. Wnts are secreted ligands that control cell processes via at least two pathways, one of which, the ‘canonical’ Wnt signalling pathway, operates through the cytosolic stabilisation of a transcriptional co-factor, β-catenin. This is achieved by downregulating the activity of a β-catenin turnover complex. Evidence from tumour expression studies, transgenic animals and in vitro experiments suggests that inappropriate activation of the canonical Wnt signalling pathway is a major feature in human neoplasia and that oncogenic activation of this pathway can occur at many levels. Inappropriate expression of the Wnt ligand and Wnt binding proteins have been found in a variety of human tumours. Further downstream, dysregulation of the β-catenin turnover complex, by loss of the Adenomatous Polyposis Coli or Protein Phosphatase 2A proteins, or by activating mutations of β-catenin, has been found in several tumour types, and is believed to be a key step in neoplastic progression. Transcriptional targets of the Wnt pathway include the cellular oncogenes cyclin D1 and c-myc. Activation of the Wnt signalling pathway by various means can therefore be a primary cause in oncogenesis, affecting cell proliferation, morphology and contact inhibition, as well as co-operating with other oncogenes in multistep tumour progression.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006369223282

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Institutional transformation at South African universities: Implications for academic staff (1999)

Fourie, Magda

Springer

Higher education 38 (1999), S. 275-290

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ISSN: 1573-174X

Keywords: academic staff ; curriculum change ; equity ; governance ; staff development ; student needs ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Nature of Science, Research, Systems of Higher Education, Museum Science

Notes: Abstract In South Africa the restructuring of the higher education system and the transformation of higher education institutions are located within the country's broad political and socio-economic transition to democracy. This paper focuses particularly on institutional transformation, and pays attention to the implications of the process of transformation for academic staff. The following five interlinked and interdependent issues characterizing institutional transformation in South African higher education are identified: democratising the governance structures of institutions increasing access for educationally and financially disadvantaged students restructuring the curriculum focusing on developmental needs in research and community service redressing inequalities in terms of race and gender. Although the overall effect of institutional transformation is experienced rather negatively by many academic staff members, the paper concludes that academics have to be empowered by means of staff development to remain active partners in the transformation process.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1003768229291

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Anaerobic transformation of 1,1,1-trichloroethane by municipal digester sludge (1999)

Chen, Chun ; Ballapragada, Bhasker S. ; Puhakka, Jaakko A. ; [et al.]

Springer

Biodegradation 10 (1999), S. 297-305

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ISSN: 1572-9729

Keywords: abiotic ; biological ; cell-free extract ; chloroethane ; dechlorination ; 1,1-dichloroethane ; 1,1-dichloroethene ; digester ; methanogenic ; transformation ; 1,1,1-trichloroethane

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract Anaerobic transformations of 1,1,1-trichloroethane (TCA), 1,1-dichloroethane (DCA), and chloroethane (CA) were studied with sludge from a lab-scale, municipal wastewater sludge digester. TCA was biologically transformed to DCA and CA and further to ethane by reductive dechlorination. TCA was also converted to acetic acid and 1,1-dichloroethene (11DCE) by cell-free extract. 11DCE was further biologically converted to ethene. This pathway was confirmed by transformation tests of TCA, DCA and CA, by tests with cell-free extract, and by chloride release during TCA degradation. With cell-free extract, acetic acid accounted for approximately 90% of the TCA transformed; tests with live cells indicate that the fraction of TCA transformed by this pathway decreased with lower biomass. The dechlorination of DCA to CA and CA to ethane was not stoichiometric. A high rate of TCA removal was observed under the experimental conditions. The results indicate that removal of TCA in anaerobic digestion should be complete, but DCA and CA could persist in a normally operating digester.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008336103968

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Urban development in East Germany — specific features of urban transformation processes (1999)

Wießner, Reinhard

Springer

GeoJournal 49 (1999), S. 43-51

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ISSN: 1572-9893

Keywords: housing market ; suburbanisation ; transformation ; urban development ; urban renewal ; East Germany

Source: Springer Online Journal Archives 1860-2000

Topics: Geography

Notes: Abstract The aim of this paper is to analyse the main characteristics of post-socialist urban development in East Germany, especially the differences compared to urban development in other East and Central European countries. In spite of the many similar problems and processes in urban development, specific features of East Germany are characterised by the rapid growth of suburbia, especially in the first phase of transition, by the proceeding activities of urban renewal and revitalisation, and by a lower level of social polarisation and socio-spatial segregation as compared to other post-socialist countries. Important conditions for urban development in East Germany exist in special support programmes, high subsidies and other financial transfers as well as in engaged planning conceptions of the local authorities.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007016912703

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Tissue culture and Agrobacterium-mediated transformation of watercress (1999)

Jin, Rong-Guan ; Liu, Yong-Biao ; Tabashnik, Bruce E. ; [et al.]

Springer

Plant cell, tissue and organ culture 58 (1999), S. 171-176

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ISSN: 1573-5044

Keywords: Bacillus thuringiensis Brassicaceae ; gene transfer ; insect resistance ; plant regeneration ; Rorippa nasturtium-aquaticum

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Adventitious shoot regeneration could be obtained from more than 80% of the calluses initiated from stem explants of watercress (Rorippa nasturtium-aquaticum) by using an induction medium and a shoot regeneration medium. The induction medium contained 1.15 μM 2,4-dichlorophenoxyacetic acid and 5 μM thidiazuron; the shoot regeneration medium was composed of 0.5 μM thidiazuron and 2.25 μM 6-benzylaminopurine. This regeneration procedure was incorporated into an Agrobacterium-mediated transformation procedure for gene transfer into watercress. Factors affecting transformation included preculture, selection agents, use of tobacco nurse cells, and the length of coculture. A transgenic line of watercress transformed with a wild-type Bacillus thuringiensis insecticidal gene, cry1Ia3, was not toxic to larvae of the diamondback moth (Plutella xylostella), presumably due to premature polyadenylation of the transcript encoded by this gene in the plant.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006399130272

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A promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants (1999)

Schenk, Peer M. ; Sagi, Laszlo ; Remans, Tony ; [et al.]

Springer

Plant molecular biology 39 (1999), S. 1221-1230

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ISSN: 1573-5028

Keywords: constitutive expression ; GFP ; GUS ; Musa ; ScBV ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A 1369 bp DNA fragment (Sc) was isolated from a full-length clone of sugarcane bacilliform badnavirus (ScBV) and was shown to have promoter activity in transient expression assays using monocot (banana, maize, millet and sorghum) and dicot plant species (tobacco, sunflower, canola and Nicotiana benthamiana). This promoter was also tested for stable expression in transgenic banana and tobacco plants. These experiments showed that this promoter could drive high-level expression of the β-glucuronidase (GUS) reporter gene in most plant cells. The expression level was comparable to the maize ubiquitin promoter in standardised transient assays in maize. In transgenic banana plants the expression levels were variable for different transgenic lines but was generally comparable with the activities of both the maize ubiquitin promoter and the enhanced cauliflower mosaic virus (CaMV) 35S promoter. The Sc promoter appears to express in a near-constitutive manner in transgenic banana and tobacco plants. The promoter from sugarcane bacilliform virus represents a useful tool for the high-level expression of foreign genes in both monocot and dicot transgenic plants that could be used similarly to the CaMV 35S or maize polyubiquitin promoter.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006125229477

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Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene (1999)

Gleave, Andrew P. ; Mitra, Deepali S. ; Mudge, Stephen R. ; [et al.]

Springer

Plant molecular biology 40 (1999), S. 223-235

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ISSN: 1573-5028

Keywords: conditional lethal dominant gene ; Cre/loxP ; Nicotiana tabacum ; site-specific recombinase ; transformation ; transient expression

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Transgenic tobacco plants were produced that contained single-copy pART54 T-DNA, with a 35S-uidA gene linked to loxP-flanked kanamycin resistance (nptII) and cytosine deaminase (codA) genes. Retransformation of these plants with pCre1 (containing 35S transcribed cre recombinase and hygromycin (hpt) resistance genes) resulted in excision of the loxP-flanked genes from the genome. Phenotypes of progeny from selfed-retransformed plants confirmed nptII and codA excision and integration of the cre-linked hpt gene. To avoid integration of the hpt gene, and thereby generate plants totally free of marker genes, we attempted to transiently express the cre recombinase. Agrobacterium tumefaciens (pCre1) was cocultivated with leaf discs of two pART54-transformed lines and shoots were regenerated in the absence of hygromycin selection. Nineteen of 773 (0.25%) shoots showed tolerance to 5-fluorocytosine (5-fc) which is converted to the toxic 5-fluorouracil by cytosine deaminase. 5-fc tolerance in six shoots was found to be due to excision of the loxP-flanked region of the pART54 T-DNA. In four of these shoots excision could be attributed to cre expression from integrated pCre1 T-DNA, whereas in two shoots excision appeared to be a consequence of transient cre expression from pCre1 T-DNA molecules which had been transferred to the plant cells but not integrated into the genome. The absence of selectable marker genes was confirmed by the phenotype of the T1 progeny. Therefore, through transient cre expression, marker-free transgenic plants were produced without sexual crossing. This approach could be applicable to the elimination of marker genes from transgenic crops which must be vegetatively propagated to maintain their elite genotype.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006184221051

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Optimisation of procedures for microprojectile bombardment of microspore-derived embryos in wheat (1999)

Ingram, H.M. ; Power, J.B. ; Lowe, K.C. ; [et al.]

Springer

Plant cell, tissue and organ culture 57 (1999), S. 207-210

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ISSN: 1573-5044

Keywords: biolistics ; gene expression ; haploid ; transformation ; Triticum aestivum

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Using the PDS-1000/He Biolistic® Particle Delivery System, the microprojectile travel distance, rupture disk pressure and DNA/gold particle concentrations were assessed in order to optimise short and longer-term β-glucuronidase reporter gene expression in microspore-derived embryos of wheat. The effects were also evaluated of using sterile filter paper to support explants and treatment with a high osmoticum medium (0.2 M mannitol/0.2 M sorbitol or 0.4 M maltose). In the optimised procedure, wheat microspore-derived embryos (MDEs), were placed on filter paper and incubated on medium containing 0.4 M maltose, for 4 h pre- and 45 h post-bombardment. Five μl pAHC25 (0.75 mg ml-1 in TE buffer) was precipitated onto 25 μl gold particles (60 mg ml-1 in sterile water), using 20 μl spermidine (0.1 M) and 50 μl CaCl2 (2.5 M). The particles were centrifuged and resuspended in 75 μl absolute ethanol prior to the preparation of 6 macrocarriers. A microprojectile travel distance of 70 mm, a rupture pressure of 1300 p.s.i., and a vacuum of 29′′ Hg were employed. Maltose at 0.4 M in the support medium was the most important factor influencing GUS activity in bombarded tissues. GUS activity, 1 day post-bombardment, reached 52 ± 17 GUS-positive foci/MDE (mean ± s.e.m, n=3), with 17 ± 4 foci/MDE at 15 days, giving a 3.0-fold increase (p〈0.05) compared to expression in MDEs bombarded on medium without a high osmoticum treatment.

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URL: http://dx.doi.org/10.1023/A:1006384525513

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Recovery of transgenic orchid plants with hygromycin selection by particle bombardment to protocorms (1999)

Yu, Zhihua ; Chen, Minyong ; Nie, Lin ; [et al.]

Springer

Plant cell, tissue and organ culture 58 (1999), S. 87-92

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ISSN: 1573-5044

Keywords: ß-glucuronidase ; dendrobium ; hygromycin phosphotransferase ; orchid ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Protocorms of orchid (Dendrobium hybrid) were transformed by microprojectile bombardment with a helium-pressured PDS 1000 particle gun. Gold particles coated with plasmid DNA containing ß-glucuronidase (GUS) and hygromycin phosphotransferase (Hpt) marker genes were used. Potentially transformed tissues were identified by active growth on MS medium supplemented with 50mg l-1 hygromycin. After 4–6 months of continuous selection, 15 hygromycin-resistant lines were recovered. Integration of transgenes into the genome of the transformed protocorms and plantlets were confirmed by GUS histochemical assay and Southern blot hybridization. The transgenic protocorms have gone through propagation for more than 8 months and maintained their transgenic characters. These results indicate that we have established a system for orchid transformation in a relatively high frequency and the transgenes are stably expressed in the transgenic plants.

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URL: http://dx.doi.org/10.1023/A:1006334605947

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Isolation of state transition mutants of Chlamydomonas reinhardtii by fluorescence video imaging (1999)

Kruse, Olaf ; Nixon, Peter J. ; Schmid, Georg H. ; [et al.]

Springer

Photosynthesis research 61 (1999), S. 43-51

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ISSN: 1573-5079

Keywords: Chlamydomonas reinhardtii ; LHC II phosphorylation ; mutagenesis ; Photosystem II redox control ; state 2 to state 1 transition ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Oxygenic photosynthetic organisms adapt to varying light conditions by changing the distribution of light energy between Photosystem II (PS II) and photosystem I (PS I) during so-called state transitions. To identify the genes involved in this process, we have exploited a simple chlorophyll fluorescence video-imaging technique to screen a library of nuclear mutants of Chlamydomonas reinhardtii for colonies grown on agar plates that are disturbed in their ability to regulate light energy distribution between PS I and PS II. Subsequent modulated fluorescence measurements at room temperature and 77 K fluorescence emission spectra confirmed that 5 mutants (0.025% of total number screened) were defective in state transitions. [32P]orthophosphate phosphorylation experiments in vivo revealed that in one of these mutants, designated stm1, the level of LHC II polypeptide phosphorylation was drastically reduced compared with wild type. Despite WT levels of PS I and PS II, stm1 grew photoautotrophically at reduced rates, compared with WT especially under low light conditions, which is consistent with an important physiological role for state transitions. Our results highlight the feasibility of video imaging in tandem with mutagenesis as a means of identifying the genes involved in controlling state transitions in eukaryotic photosynthetic organisms.

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URL: http://dx.doi.org/10.1023/A:1006229308606

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Simple method for plasmid mediated transformation of different Bacillus species (1999)

Martinez, M. Alejandra ; Dezar, Carlos ; Baigorí, Mario ; [et al.]

Springer

Biotechnology techniques 13 (1999), S. 337-340

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ISSN: 1573-6784

Keywords: Bacillus ; plasmids ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract A simple and easy method for the introduction of plasmid DNA into different species of Bacillus was developed. The method involves the suspension in a transformation buffer of nutrient agar grown cells in their late exponential phase and the addition of unpurified plasmid DNA. Transformants were obtained at a frequency of about 103 to 105 stable transformants per μg of plasmid DNA.

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URL: http://dx.doi.org/10.1023/A:1008941007983

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Intracellular fate and nuclear targeting of plasmid DNA (1999)

Neves, C. ; Escriou, V. ; Byk, G. ; [et al.]

Springer

Cell biology and toxicology 15 (1999), S. 193-202

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ISSN: 1573-6822

Keywords: DNA ; gene transfer ; importin ; nuclear import ; nuclear localization signal

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract One of the major steps limiting nonviral gene transfer efficiency is the entry of plasmid DNA from the cytoplasm into the nucleus of the transfected cells. The nuclear localization signal (NLS) of the SV40 large T antigen is known to efficiently induce nuclear targeting of proteins. We have developed two chemical strategies for covalent coupling of NLS peptides to plasmid DNA. One method involves a site-specific labeling of plasmid DNA by formation of a triple helix with an oligonucleotide–NLS peptide conjugate. After such modification with one NLS peptide per plasmid molecule, plasmid DNA remained fully active in cationic lipid-mediated transfection. In the other method, we randomly coupled 5–115 p-azidotetrafluorobenzyllissamine–NLS peptide molecules per plasmid DNA by photoactivation. Oligonucleotide–NLS and plasmid–lissamine–NLS conjugates interacted specifically with the NLS-receptor importin α. Plasmid–lissamine–NLS conjugates were not detected in the nucleus, after cytoplasmic microinjection. Plasmids did not diffuse from the site of injection and plasmid–lissamine–NLS conjugates appeared to be progressively degraded in the cytoplasm. The process of plasmid DNA sequestration/degradation stressed in this study might be as important in limiting the efficiency of nonviral gene transfer as the generally recognized entry step of plasmid DNA from the cytoplasm into the nucleus

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URL: http://dx.doi.org/10.1023/A:1007693805849

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Long-Term Transformation and Redistribution of Potentially Toxic Heavy Metals in Arid-Zone Soils: II. Incubation at the Field Capacity Moisture Content (1999)

Han, Feng Xiang ; Banin, Amos

Springer

Water, air & soil pollution 114 (1999), S. 221-250

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ISSN: 1573-2932

Keywords: arid-zone soils ; field capacity ; fractionation ; heavy metals ; kinetics ; redistribution ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Energy, Environment Protection, Nuclear Power Engineering

Notes: Abstract Solid-phase transformation of added Cd, Cu, Cr, Ni, Pb and Zn, in two arid-zone soils incubated in the field capacity moisture regime for one year, were studied. The heavy metals were fractionated into six empirically defined fractions using a selective sequential dissolution (SSD) protocol optimized for arid-zone soils. Each of these fractions was named based on the major soil component targeted for dissolution during the specific SSD step, but it is not assumed that they are mineralogically and chemically totally specific. The transformations of the metals in the two soils incubated at the field capacity regime were compared with those at the moisture saturation regime (Han and Banin, 1997). An initial fast stage of transformation of the soluble metals from the exchangeable (EXC) fraction to the less labile fractions (the carbonate (CARB) fraction for Cd, Pb, Zn, Ni and Cu, and the organic matter (OM) fraction for Cr, and to some extent Cu and Ni) occurred during the fractionation and within one hour after addition. This was followed by a second stage, involving long-term transformation processes of all metals: added Cd was transferred from the EXC into the CARB fraction; added Cr was transferred from the CARB to the OM fraction and Pb was transferred very slowly to the easily reducible oxide (ERO) fraction. Added Cu, Ni and Zn were transferred from the EXC and CARB fractions into the ERO fraction and to some extent OM and RO fractions. In Part I of this series, we reported that during incubation in the saturated moisture regime, Zn and Ni were transferred mainly into the RO and OM fractions. Cadmium, Cr and Pb underwent the same transformation pathways during the slow long-term process, with slightly different rates, in both water regimes. At low levels of addition, the incubated soils moved over one year towards a distribution similar to that of the native soil. At higher levels, the soils still remained removed from the quasi-equilibrium which characterized the native soil, even at the end of one year of incubation.

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URL: http://dx.doi.org/10.1023/A:1005006801650

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A Novel Non-Viral Vector for DNA Delivery Based on Low Molecular Weight, Branched Polyethylenimine: Effect of Molecular Weight on Transfection Efficiency and Cytotoxicity (1999)

Fischer, Dagmar ; Bieber, Thorsten ; Li, Youxin ; [et al.]

Springer

Pharmaceutical research 16 (1999), S. 1273-1279

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ISSN: 1573-904X

Keywords: gene transfer ; cytotoxicity ; polyethylenimine ; polyfection

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract Purpose. Low molecular weight branched polyethylenimine (LMW-PEI) was synthesized and studied as a DNA carrier for gene delivery with regard to physico-chemical properties, cytotoxicity, and transfection efficiency. Methods. The architecture of LMW-PEI, synthesized by acid catalyzed ring-opening polymerization of aziridine was characterized by size exclusion chromatography in combination with laser light scattering and 13C-NMR-spectroscopy. In vitro cytotoxic effects were quantified by LDH and MTT assay and visualized by transmission electron microscopy. The potential for transgene expression was monitored in ECV304 cells using luciferase driven by a SV40 promoter as reporter gene system. Results. LMW-PEI (Mw 11′900 D) with a low degree of branching was synthesized as a DNA carrier for gene delivery. In contrast to high molecular weight polyethylenimines (HMW-PEI; Mw l′616′OOO D), the polymer described here showed a different degree of branching and was less cytotoxic in a broad range of concentrations. As demonstrated by transmission electron microscopy the LMW-PEI formed only small aggregates which were efficiently taken up by different cells in the presence of serum, most likely by an endocytic pathway. LMW-PEI yielded transfection efficiencies measured via expression of the reporter gene luciferase which were up to two orders of magnitude higher than those obtained with HMW-PEI. The reporter gene expression was concentration dependent, but in contrast to lipofection independent of serum addition. Conclusions. The LMW-PEI described here is a new, highly efficient, and non-cytotoxic vector with a favorable efficiency/toxicity profile for gene therapeutic applications.

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URL: http://dx.doi.org/10.1023/A:1014861900478

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Sorption Kinetics and Transformation of DDT in Sediment (1999)

van den Hoop, Marc A. G. T. ; Kreule, Petra ; Gustav Loch, J. P.

Springer

Water, air & soil pollution 110 (1999), S. 57-66

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ISSN: 1573-2932

Keywords: DDT ; kinetic ; organic pollutant ; sediment ; sorption ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Energy, Environment Protection, Nuclear Power Engineering

Notes: Abstract The overall objective of this study was to investigate the sorption kinetics of DDT in sediment under similar experimental conditions employed in corresponding toxicity studies for bentic organisms. A batch of aerated Schoonrewoerdse Wiel sediment, initially spiked with DDT, was sampled over a period of seven days. Concentrations of DDT, DDD and DDE were determined in both the solid and the solution phase in the sediment/water system after separation by centrifugation. It was found that the extractable amount of DDT decreased with increasing contact time. This can partly be explained in terms of transformation of DDT into DDD. Furthermore, the present applied extraction procedure seems to be less effective with increasing contact time, indicating an increase in binding strength of DDT with the sediment material. Finally, on the basis of DDT, DDE and DDD concentrations in both the solid phase and the solution phase, partition coefficients were calculated, which appeared to be independent of the contact time. This points at a very rapid equilibrating between DDT in pore water and in the extractable forms adsorbed at the solid phase.

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URL: http://dx.doi.org/10.1023/A:1005087429581

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Environmental Risk Assessment for Pesticides in the Atmosphere; The Results of an International Workshop (1999)

Guicherit, Robert ; Bakker, Dick J. ; de Voogt, Pim ; [et al.]

Springer

Water, air & soil pollution 115 (1999), S. 5-19

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ISSN: 1573-2932

Keywords: atmospheric fate ; atmospheric transport ; deposition ; emission ; long-range transport ; pesticides ; registration ; remote area ; risk assessment ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Energy, Environment Protection, Nuclear Power Engineering

Notes: Abstract The Health Council of the Netherlands organised an international workshop on the fate of pesticides in the atmosphere and possible approaches for their regulatory environmental risk assessment. Approximately forty experts discussed what is currently known about the atmospheric fate of pesticides and major gaps in our understanding were identified. They favoured a tiered approach for assessing the environmental risks of atmospheric dispersion of these chemicals. In the first tier a pesticide's potential for emission during application, as well as its volatilisation potential should be assessed. Estimates of the former should be based on the application method and the formulation, estimates of the latter on a compound's solubility in water, saturated vapour pressure and octanol/water partition coefficient. Where a pesticide's potential for becoming airborne exceeds critical values, it should be subjected to a more rigorous second tier evaluation which considers its toxicity to organisms in non-target areas. This evaluation can be achieved by calculating and comparing a predicted environmental concentration (PEC) and a predicted no-effect concentration (PNEC). By applying an extra uncertainty factor the PNEC can be provisionally derived from standard toxicity data that is already required for the registration of pesticides. Depending on the distance between the source and the reception area, the PEC can be estimated for remote areas using simple dispersion, trajectory type models and for nearby areas using common dispersion models and standard scenarios of pesticide use. A pesticide's atmospheric transport potential is based on factors such as its reaction rate with OH radicals. It should be used to discriminate between those compounds for which only the risks to nearby ecosystems have to be assessed, and those for which the risks to remote ecosystems also have to be determined. The participants were of the opinion that this approach is, in principle, scientifically feasible, although the remaining uncertainties are substantial. Further field and laboratory research is necessary to gain more reliable estimates of the physico-chemical properties of pesticides, to validate and improve environmental fate models and to validate the applicability of standard toxicity data. This will increase both the accuracy of and our confidence in the outcome of the risk assessment.

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URL: http://dx.doi.org/10.1023/A:1005238703698

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Evolution of gene transfer in bacteria (1999)

Trevors, J.T.

Springer

World journal of microbiology and biotechnology 15 (1999), S. 1-6

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ISSN: 1573-0972

Keywords: Bacteria ; conjugation ; DNA ; evolution ; gene transfer ; transduction ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract The transfer of genetic information by transformation, conjugation and transduction in bacteria occurs frequently in nature. These diverse gene transfer mechanisms in bacteria are the result of evolution and are not linked to reproduction as in eukaryotic organisms. In this review, gene transfer in bacteria will be considered from an evolutionary perspective.

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URL: http://dx.doi.org/10.1023/A:1008830914223

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Biotransformation of cholesterol by Micrococcus species mediated by plasmid DNA (1999)

Dogra, N. ; Qazi, G.N.

Springer

World journal of microbiology and biotechnology 15 (1999), S. 411-415

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ISSN: 1573-0972

Keywords: Electroporation ; Micrococcus species ; steroid biotransformation ; transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract A steroid-biotransforming strain RJ6 was identified as Micrococcus roseus. This bacterium has a 10 kb plasmid pMQV10. Curing mediated through cultivation of the culture with a low concentration (200 ng/ml) of mitomycin C is described. Loss of cholesterol degradation (chol+) and streptomycin resistance (Smr) phenotypes as a consequence of the loss of plasmid indicate the extrachromosomal location of these two genes in this strain. An electroporation procedure was developed for transformation of cured strain of Micrococcus (RJC6) by plasmids. Frequency of greater than 105 transformants/μg DNA was achieved, which is 100-fold higher than the standard transformation procedure that yielded 5.3×103 transformants/μg DNA in the same strain.

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URL: http://dx.doi.org/10.1023/A:1008990910303

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Airflow and temperature distribution in two-dimensional drying bins (1999)

Peng, W. ; Smith, E. A. ; de Ville, A.

Springer

Journal of engineering mathematics 36 (1999), S. 241-254

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ISSN: 1573-2703

Keywords: flows in porous media ; transformation ; heat transfer ; drying bins ; conformal mapping.

Source: Springer Online Journal Archives 1860-2000

Topics: Mathematics , Technology

Notes: Abstract The design of a drying or cooling store aims to provide an even airflow distribution, when aerated, for preservation purposes. The airflow in some curved bottom bins are studied in this paper. The flow is modelled, using Darcy's law. A generalized Schwarz-Christoffel transformation is employed to reduce the problem of computing streamlines and isobars of airflow to solving a single nonlinear equation for the flow angle along the wall. Corresponding to different bin shapes, a few computed streamlines and isobars of airflow are presented, showing the effect of changing bottom geometries on the air flow. Heat transfer in such bins is also investigated. Based on an analysis of the far field of airflow, finite-height bins are considered. Analytical solutions of the heat conduction equation in terms of streamlines and isobars are obtained.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1004488010532

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Changes in morphology, cytoskeleton, and substrate dependence of proliferation after transfection of immortalized rat embryonic fibroblasts with E7 gene of type 16 human papilloma virus (1999)

Zhurbitskaya, V. A. ; Rovenskii, Yu. A. ; Kaverina, I. N.

Springer

Bulletin of experimental biology and medicine 127 (1999), S. 99-102

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ISSN: 1573-8221

Keywords: human papilloma virus ; transformation ; actin ; fibronectin

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Transfection of rat embryonic fibroblasts with E7 gene of type 16 human papilloma virus changed the cytoskeleton and cell-cell and cell-matrix interactions in two clones of transformed cells. Cell morphology and substrate-dependent proliferation were also changed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02432813

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Masthead (1999)

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999)

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710301

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Nanofiltrationsmembranen für Trennprobleme in organischen Lösungen (1999)

Schmidt, Matthias ; Mirza, Sohail ; Schubert, Rolf ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 199-206

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Nanofiltration Membranes for Separation Problems in Organic Solutions.Nanofiltration based on rejection and flux features intermediate between those ultrafiltration and reverse osmosis is one of the technically and scientifically interesting membrane processes with a great future. Moreover, most commercial available nanofiltration membranes are only suitable for separation processes in aqueous solutions. A small number of composite membranes with a highly cross-linked selective layer show a technically interesting resistance towards organic solvents, such as ketones, esters, ethers or alcohols. The present article describes such nanofiltration membranes used for separation of low molecular weight chemical compounds from polar or nonpolar solvents, but also for the removal of organic compound from aqueous solutions. Methods of manufacturing and modifying such solvent stable composite membranes are shown, as are the conditioning of membranes and examples of industrial application.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710304

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Seitliche Reaktandenzufuhr in großmaßstäblichen Wirbelschichtreaktoren - Modellierung am Beispiel der Sekundärlufteinblasung in WirbelschichtfeuerungenVorgetragen von T. KNÖBIG anläßlich der internen Arbeitssitzung des Fachausschusses „Energieverfahrenstechnik“, 20./22. April 1998 in Würzburg. (1999)

Knöbig, Thomas ; Werther, Joachim

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 219-222

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710307

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Numerische Simulation der Strömungsvorgänge und des Trennverhaltens von Suspensionen im freien Einzelspalt eines TellerseparatorsVortrag auf der GVC-Tagung „Mechanische Flüssigkeitsabtrennung“, 25. März 1998 in Freiburg. (1999)

Janoske, Uwe ; Piesche, Manfred

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 249-253

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710315

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CAPE in der Praxis - Möglichkeiten und Grenzen aus NutzersichtVortrag anläßlich der GVC-Jahrestagung, 24./26. Sept. 1997 in Dresden. (1999)

Lohe, B. ; Bessling, B. ; Schoenmakers, H. ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 323-327

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: CAPE in Practice - Opportunities and Limits from the User's Point of View.CAPE tools comprise computer based tools and methods for the design of chemical processes - the use of which is indispensable in modern chemical engineering. This paper discusses the degree to which CAPE is already applied and whether limitations exist. Within BASF, the CAPE framework is provided by the “ProcessNet”, which integrates numerous simulation and design tools. The engineer relies alternatively on inhouse or commercial software. The advantage of CAPE in terms of efficiency depends not only on complex programs, but also to a large extent on the multiple use of intermediate data and program modules. The ProcessNet also features a hierarchical file structure to support the engineer's desktop management. The CAPE environment is being continuously improved. The main constraints on CAPE today are, for example, the missing link between fluid and solid applications, a deficit in data management and the poor state of development of advanced or special programs. The progress of research in this area is followed with great interest.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710403

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Einfluß der Fluidisierungsbedingungen auf Abscheidung und Agglomeration von Aerosolen beim Durchgang durch Wirbelschichten (1999)

Fleck, Sabine ; Riebel, Ulrich

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 361-364

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710409

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Modifizierung der Partikelwechselwirkung durch Beschichtung mit grenzflächenaktiven ReagenzienÜberarbeiteter Vortrag anläßlich der Sitzung des GVC-Fachausschusses „Grenzflächen“, 23. März 1998 in Freiburg. (1999)

Wolf, Regine ; Hoffmann, Bernd ; Wahl, Beate ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 372-376

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710412

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Untersuchung zur Hydrodynamik und Kinetik an KATAPAK-Packungen (1999)

Brehm, Axel ; Antons, Uwe ; Bekurdts, Alexander

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 388-392

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710416

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Konzentrationsprogrammierte Reaktionstechnik - Eine Methode zur Beurteilung des Anwendungspotentials instationärer Prozeßführungen bei PartialoxidationenTeil eines Vortrags anläßlich der 3. Merseburger Fachtagung „Automatisierung, Meßmethoden und Experimente in der chemischen Reaktionstechnik“, 6. Nov. 1997. (1999)

Böhling, Ralf ; Drochner, Alfons ; Fehlings, Michael ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 226-230

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710309

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Zur Kopplung von Impuls- und Stofftransport an fluiden Phasengrenzen - Teil 1: TheorieVortrag (gekürzt) anläßlich der GVC-Jahres-tagung, 24./26. Sept. 1997 in Dresden. (1999)

Tokarz, Alexander ; Millies, Marco ; Mewes, Dieter

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 472-477

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710510

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VerGrößerung der spezifischen Oberfläche von Kristallen durch gezieltes Nutzen von PseudopolymorphenErweiterte Fassung eines Vortrags von ST. NORDHOFF (Vortragender) und J. ULRICH anläßlich der internen Arbeitssitzung des GVC-Fachauschusses „Kristallisation“, 2./6. März 1998 in Aachen. (1999)

Nordhoff, Stefan ; Dümpelmann, Ralf ; Wagner, Gerhard ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 487-490

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710514

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Einfluß der Dimerisierung von Isobuten auf die MTBE-Produktion bei der reaktiven Destillation (1999)

Gravekarstens, Manfred ; Hoffmann, Ulrich

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 500-503

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710518

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Chemical Reaction and Reactor Design (1999)

Chuang, Steven

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 518-518

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710523

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Processing of Particulate Solids (1999)

Schwedes, I.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 520-520

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710526

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GVC-Fachausschuß „Prozeß-und Anlagentechnik“ (1999)

Bathen, Dieter

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 516-517

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Die Sitzung des GVC-Fachausschusses „Prozeß- und Anlagentechnik“ fand am 25. und 26. Oktober 1998 in Kleve statt. Gemeinsam mit dem DECHEMA-Ausschuß „Computeranwendungen in der chemischen Industrie“ und dem GVC-Arbeitskreis „Produktionslogistik“ wurden aktuelle Problemstellungen aus der Industrie und neue Problemlösungsansätze aus der Forschung präsentiert und diskutiert.In seiner kurzen Begrüßung erläuterte der Ausschußvorsitzende H. SCHMIDT-TRAUB einerseits die Zielsetzung der Tagung und verdeutlichte andererseits den Teilnehmern die Ursachen und Konsequenzen des drastischen Rückgangs der Anfängerzahlen in den verfahrenstechnischen Studiengängen, was für Gesprächsstoff in den Pausen sorgte. Die anschließende Tagung umfaßte insgesamt 35 Vorträge in zehn Schwerpunktthemen.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710522

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Abwasser (1999)

Frimmel, F. H.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 523-524

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710532

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Aktuelles (1999)

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 531-553

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710602

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Abtrennen von Wertstoffen aus BauschuttVortrag anläßlich der GVC-Jahrestagung, 30. Sept./2. Okt. 1998 in Freiburg. (1999)

Tomas, Jirgen ; Gröger, Torsten ; Küchler, Christoph ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 637-642

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710619

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Wärmeintegration bei Batch-Prozessen (1999)

Uhlenbruck, Stefan ; Vogel, Rainer ; Lucas, Klaus

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 700-704

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710710

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Rheologische Eigenschaften von wäßrigen nicht-ionischen Tensidlösungen in stationärer ScherströmungVortrag anläßlich der Sitzung des GVC-Fachausschusses „Rheologie“, 2./6. März 1998 in Aachen. (1999)

Teipel, Ulrich ; Aksel, Nuri

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 720-725

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 7 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710715

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Systematische vergleichende Kostenanalyse von destillativen und extraktiven Stofftrennverfahren (1999)

Glanz, Stephan ; Blaß, Eckhart ; Stichlmair, Johann ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 669-673

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Systematic Comparative Cost Analysis of Separation Processes Based on Distillation and Extraction.Rectification is the most commonly used but not always the most economical method of thermal separation. Above all in the case of closely boiling mixtures it becomes very demanding with regard to both equipment and energy. It is often possible to replace rectification by another energetically more favourable separation process such as liquid-liquid distillation, decantation, or absorption. Owing to the resulting wealth of possibilities for the separation of a mixture it is desirable to gain further knowledge about the economics of individual processes and about the selection of suitable entrainers under given conditions.

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710703

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Die Hydrolyse von HCN als Zwischenschritt bei der Stickoxidbildung (1999)

Schäfer, S. ; Bonn, B.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 613-618

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 8 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710614

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Oxidische Schichten auf EisenChrom-Nickel-Werkstoffen und ihre chemischen Eigenschaften (1999)

Koppe, Jürgen ; Lausch, Hart Mut ; Heubner, Ulrich ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 609-612

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 6 Tab.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710613

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Verfahren zum Abbau von organischen Schadstoffen durch indirekte Elektrooxidation (1999)

Galla, Ulrich ; Kritzer, Peter ; Bringmann, Jan ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 628-633

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 7 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710617

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Kontinuierliche Dosierung und Dispergierung feindisperser, kohäsiver Feststoffe mit einem neuartigen Staubdosierer (1999)

Dittler, Achim ; Kasper, Gerhard

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 685-688

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710706

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Numerische Untersuchung der Strömungsverhältnisse im Innern von Hohlkegeldüsen mit Leitapparat (1999)

Nonnenmacher, Stefan ; Piesche, Manfred

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 688-692

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710707

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Kinetik der Umesterung von Ethanol und Butylacetat - Ein Modellsystem für die Reaktivrektifikation (1999)

Schmidt, Jörg ; Reusch, Dieter ; Elgeti, Klaus ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 704-708

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710711

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BRICK - Ein 1-D-Simulationstool für Mehrphasenströmungen in Behältern (1999)

Lucas, Dirk

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 713-717

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710713

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Vergleichende Untersuchungen zur nichtadiabaten Absorption im Film- und Blasen-KolbenregimeÜberarbeitete Fassung eines Postervortrages von U. HELBING anläßlich der GVC-Jahrestagung, 30. Sept./02. Okt. 1998 in Freiburg. (1999)

Helbing, Uwe ; Würfel, Reinhard ; Fratzscher, Wolfgang

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 844-849

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Additional Material: 7 Ill.

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URL: http://dx.doi.org/10.1002/cite.330710813

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Möglichkeiten zur Vorausberechnung von Verweilzeitverteilungen (1999)

Platzer, Bernd ; Steffani, Katharina ; GroßE, Stefan

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 795-807

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Notes: Possible Approaches to the Prediction of Residence Time Distributions Although the experimental determination of residence time distributions is based on a black-box method, the signal shape also permits certain conclusions to be drawn about the internal conditions. However, if it is to provide a sole basis for elucidating process steps in a plant this method is soon overtaxed. A deeper theoretical penetration of the pertinent transport processes opens up the way ahead. However, since residence time distributions are based on mass-oriented considerations, substantial advances in fluid mechanics, where location-based considerations predominate, have not led to improved predictive capability. Yet the computer power now available offers a variety of ways of calculating residence-time distributions for a known flow profile, thus permitting a step in the direction of better predictability of residence time distributions. This article presents utilisable methods and evaluates them with regard to their demands on time and effort and their predictive power. Problems arising in connection with the models are discussed.

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Dreiphasenrektifikation in Packungskolonnen (1999)

Siegert, Markus ; Stichlmair, Johann ; Repke, Jens-Uwe ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 819-823

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Einfluß von Polyelektrolytkomplexschichten auf Trennleistung und Foulingverhalten oberflächen- und bulkmodifizierter MembranenPosterbeitrag anläßlich des 7. Aachener Membran Kolloquiums, 9./11. März 1999 in Aachen. (1999)

Meier-Haack, Jochen ; Rieser, Theresia ; Lenk, Wolfgang ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 839-844

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In-situ-Bestimmung momentaner Reaktionsraten von Brennstoffpartikeln durch simultane Messung von Partikeltemperatur und GrößeÜberarbeitete und ergänzte Version des Vortrages von T. REICHELT anläßlich der GVC-Jahrestagung, 30. Sept./2. Okt. 1998 in Freiburg. (1999)

Reichelt, Tobias ; Spliethoff, Hartmut ; Hein, Klaus R. G.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 857-861

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Parallelisierte Synthese und schnelle katalytische Testung von Katalysatorbibliotheken für Oxidationsreaktionen (1999)

Rodemerck, Uwe ; Ignaszewski, (Fh) Peter ; Lucas, (Fh) Martin ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 873-877

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GVC-Fachausschuß „Gasreinigung“Berichterstatter: Dipl.-Ing. ACHIM DITTLER, Institut für Mechanische Verfahrenstechnik und Mechanik, Universität Karlsruhe (TH), D-76128 Karlsruhe, und Priv.-Doz. Dr.-Ing. EBERHARD SCHMIDT, DEGUSSA-HÜLS AG, D-63403 Hanau (1999)

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 882-883

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Notes: Die diesjährige interne Sitzung des GVC-Fachausschusses „Gasreinigung“ wurde zusammen mit der Arbeitssitzung des Fachausschusses „Energieverfahrenstechnik“ am 19. und 20. April im Tagungszentrum „Festung Marienburg“ in Würzburg abgehalten.

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Lime und Limestone. J.A.H. Oates Wiley-VCH, Weinheim 1998, 455 Seiten, 79 Abbildungen, 86 Tabellen, geb. DM 298,- ISBN 3-527-29527-5 (1999)

Oppermann, B. ; Esser, P.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 889-889

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124. Bestimmung von Trennschärfegraden bei Klassierern zum naßmechanischen Recycling von Betonrestmassen (1999)

Ressel, O. ; Stark, U. ; Müller, A.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1024-1025

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URL: http://dx.doi.org/10.1002/cite.3307109128

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128. Echtzeit-Standard-Infrastrukturkonzept für die Integration von Daten, Applikationen und Präsentationen (1999)

Schneider, Herbert

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1026-1026

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132. Simulationsbasierte intelligente Optimierung in der Prozeßindustrie (1999)

Krug, W. ; Liebelt, J.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1029-1029

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URL: http://dx.doi.org/10.1002/cite.3307109136

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140. Strukturwandel im Anlagenbau: Von der Projektierung bis zur Instandhaltung (1999)

Mohrenz, H.-J. ; Bornträger, Carsten

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1035-1035

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108. Trocknung und Pyrolyse von Holz - Flüssigkeits- und Gastransport im porösen Partikel (1999)

Wolfinger, Martin Georg ; Steiner, Gerhard ; Rath, Johannes ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1013-1014

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113. Homogenisierung des Feeds und Verbesserung des Strömungsprofils im Reaktoreintritt von Salpetersäureanlagen durch neuartige Strömungseinbauten (1999)

Schneider, Gottlieb

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1016-1017

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152. Fluoreszenzsondentechnik zum Stoffübergang in der Reaktivextraktion (1999)

Mörters, M. ; Bart, H.-J.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1043-1043

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155. Aufarbeitung von Kühlschmierstoffen im Festbett-Umlaufreaktor (1999)

Wisst, S. ; Trick, I. ; Sternad, W. ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1045-1045

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156. NF und RO zur Kreislaufführung von Laugen und Nachspülwässern in Flaschenwaschmaschinen (1999)

Rögener, F. ; Mavrov, V. ; Chmiel, H.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1046-1046

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159. Absorption von NOX-Gasen in wäßrigen Lösungen mit Hilfe von H2O2 (1999)

Sowade, T. ; Ham, R. ; Elgeti, K.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1047-1047

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URL: http://dx.doi.org/10.1002/cite.3307109163

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164. Abgasnachbehandlung durch Reaktivfiltration (1999)

ClaußEn, Michael ; Gärtner, Peter

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1050-1050

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URL: http://dx.doi.org/10.1002/cite.3307109168

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134. Zurückgezogen (1999)

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1030-1030

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137. Simulation der Strömung und des Wärmeaustauschs in und um Katalysatorformkörpers (1999)

Mindt, H.-W. ; Megahed, M.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1030-1031

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172. Mobilisierung und Austrag von Mineralölkohlenwasserstoffen bei der In-situ-Bodensanierung unter Anwendung von HF-Energie und Bodenluftabsaugung (1999)

Müller, S. ; Struppe, H. G. ; Roland, U.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1055-1056

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174. Thermowaage mit intelligenter Gasdosiereinheit zur reaktionstechnischen Untersuchung heterogener Katalysatoren (1999)

Schnitzlein, K. ; Otto, O.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1056-1056

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179. Anwendung von Röntgen-Computertomographie bei Zweiphasenströmungen (1999)

Simon, M. ; Cherdron, W. ; Cacuci, H. C. D. G.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1060-1060

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182. Strömungs- und Transportvorgänge in Rechteckkanälen mit gekreuzten Gitterstrukturen (1999)

Gschwind, P. ; Kottke, V. ; Zimmerer, C. C.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1062-1063

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189. Ingenieure in Forschung und Entwicklung (1999)

Plöcker, Ulf ; Bode, Henning ; Schönborn, J.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1068-1069

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190. Alternative Möglichkeiten zur Studienfinanzierung (1999)

Bauer, Michael

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1069-1069

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194. Datenbankbasierte Informationssysteme zur Unterstützung von Verfahrensentwicklung, -bearbeitung und -dokumentation (1999)

Neef, Oliver

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 1070-1071

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Masthead (1999)

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999)

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1. Kaltes Plasma zur Oxidation von flüchtigen Kohlenwasserstoffen (1999)

Prölss, Julian ; Modigell, Michael

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 925-926

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5. Manufacture of Nanoparticles (1999)

Pratsinis, S. E.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 929-930

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8. Nano-Prozeßtechnologie für die Herstellung von halbleitenden Nano-Partikeln (1999)

Kruis, F. E. ; Fissan, H.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 932-932

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12. Korrelation zwischen Reaktivität und Oberflächenzustand von gasgetragenen Nickel-Nanopartikeln am Beispiel der Methanbildung (1999)

Weber, Alfred P. ; Seipenbusch, Martin ; Kasper, Gerhard

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 936-937

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16. Systematische Prozeßsynthese mit Hilfe mathematischer Methoden (1999)

Brusis, D. ; Frey, Th. ; Stichlmair, J. ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 940-941

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20. Einflußfaktoren bei der Modellierung der Flüssig/Flüssig-Extraktion basierend auf Laborversuchen (1999)

Henschke, M. ; Pfennig, A.

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 944-944

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710924

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9. Partikelpopulationsbilanzen: Numerische Simulation der Partikelsynthese (1999)

Mühlenweg, Heike ; Gutsch, Andreas ; Schild, Andreas ; [et al.]

Weinheim : Wiley-Blackwell

Chemie Ingenieur Technik - CIT 71 (1999), S. 932-933

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ISSN: 0009-286X

Keywords: Chemistry ; Industrial Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/cite.330710913

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Paper (German National Licenses)

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