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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Histopathology 26 (1995), S. 0 
    ISSN: 1365-2559
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: We report a retrospective study of the pathological features in 69 primary pulmonary non-Hodgkin's lymphomas which have previously been clinically reviewed. The tumours consisted of 61 (88%) low-grade and eight (12%) high-grade malignant lymphomas. Fifty-four of the low-grade malignant lymphomas were MALT lymphomas. Lymphoepithelial lesions were observed in bronchial, bronchiolar and alveolar lining. All tumours were composed of nodules, forming a lymphangitic pattern at the periphery and a confluent central mass. Invasion of pleura and vessels was often seen but this without any consequence on survival. Granulomas were found in 20% of cases. Six of the eight high-grade tumours were centroblastic and another two were B-cell lymphomas of undetermined type. In four cases, associated areas of low-grade malignant lymphoma with lympho-epithelial lesions indicated a preexisting MALT lymphoma. Clinical data suggest that limited surgery or non-aggressive chemotherapy can provide long-term survival in patients with such slowly developing neoplasms. However, non-invasive diagnostic methods need to be developed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 353 (1995), S. 64-72 
    ISSN: 1432-1912
    Schlagwort(e): Sheep cardiac Purkinje fibre ; Voltage-clamp ; Pacemaker current ; Use dependence ; Specific bradycardic agent ; ZD 7288
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The inhibition of the pacemaker current (i f) in sheep cardiac Purkinje fibres by ZD 7288 [4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino)pyrimidinium chloride] is lost use-dependently. This disinhibition of i f was investigated by using the two-microelectrode voltage-clamp technique. The pulse protocol consisted of a rest period (holding potential of about -50 mV, 1–10 μmol/l ZD 7288) followed by a train of test pulses (potential negative to -100 mV, stimulation frequency 0.05 Hz). At the beginning of the first test pulse there was an immediate reduction of i f but inhibition was lost during continued stimulation. Activation of i f is sigmoidal and the early delay in current activation was prolonged from 33 ms (no ZD 7288) to 424 ms (10 μmol/l ZD 7288). Therefore hardly any disinhibition occurred during short test pulses (0.5 s). During longer test pulses (5 s, -120 mV, 10 μmol/l) disinhibition developed with a time constant of about 2 s. The inhibition of i f by ZD 7288 was lost voltage-dependently. With 10 μmol/l ZD 7288 the half-maximal disinhibition occurred at -92 mV and the slope factor of the disinhibition/voltage curve (Boltzmann relation) was 4.8 mV. The voltage-dependent disinhibition could be abolished largely by extracellular application of protease (0.5 mg/ml, 7 min). After prior disinhibition, reinhibition at the holding potential (about -50 mV) followed a bi-exponential time course indicating that inhibition may be produced by a fast (τ=0.7 min) and a slow component (τ=20–30 min). Increasing ZD 7288 concentration from 1 to 10 μmol/l accelerated reinhibition, mainly by an increase of the amplitude (A) of the fast component. The ratio A fast/A sIow was 0.399 at 1 μmol/l and 2.65 at 10 μmol/1 ZD 7288. The reinhibition of i f was unchanged by shifting the holding potential from -50 mV to -20 mV Trials to wash out the effects of 10 μmol/l ZD 7288 gave two results. The inhibition of i f was slightly reversed after a wash-out of 1.5 h with drug-free solution. A second effect of the drug, the fast reinhibition, could be completely removed by washout. In summary i f is inhibited by ZD 7288 at membrane potentials at which the virtual i f gate is closed. Disinhibition occurs during long-lasting hyperpolarization but will hardly be operative in unclamped fibres under physiological conditions.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 353 (1995), S. 64-72 
    ISSN: 1432-1912
    Schlagwort(e): Key words Sheep cardiac Purkinje fibre ; Voltage-clamp ; Pacemaker current ; Use dependence ; Specific bradycardic agent ; ZD 7288
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The inhibition of the pacemaker current (i f) in sheep cardiac Purkinje fibres by ZD 7288 [4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino)pyrimidinium chloride] is lost use-dependently. This disinhibition of i f was investigated by using the two-microelectrode voltage-clamp technique. The pulse protocol consisted of a rest period (holding potential of about –50 mV, 1–10 μmol/l ZD 7288) followed by a train of test pulses (potential negative to –100 mV, stimulation frequency 0.05 Hz). At the beginning of the first test pulse there was an immediate reduction of i f but inhibition was lost during continued stimulation. Activation of i f is sigmoidal and the early delay in current activation was prolonged from 33 ms (no ZD 7288) to 424 ms (10 μmol/l ZD 7288). Therefore hardly any disinhibition occurred during short test pulses (0.5 s). During longer test pulses (5 s, –120 mV, 10 μmol/l) disinhibition developed with a time constant of about 2 s. The inhibition of i f by ZD 7288 was lost voltage-dependently. With 10 μmol/l ZD 7288 the half-maximal disinhibition occurred at –92 mV and the slope factor of the disinhibition/voltage curve (Boltzmann relation) was 4.8 mV. The voltage-dependent disinhibition could be abolished largely by extracellular application of protease (0.5 mg/ml, 7 min). After prior disinhibition, reinhibition at the holding potential (about –50 mV) followed a bi-exponential time course indicating that inhibition may be produced by a fast (τ=0.7 min) and a slow component (τ=20–30 min). Increasing ZD 7288 concentration from 1 to 10 μmol/l accelerated reinhibition, mainly by an increase of the amplitude (A) of the fast component. The ratio A fast/A slow was 0.399 at 1 μmol/l and 2.65 at 10 μmol/l ZD 7288. The reinhibition of i f was unchanged by shifting the holding potential from –50 mV to –20 mV. Trials to wash out the effects of 10 μmol/l ZD 7288 gave two results. The inhibition of i f was slightly reversed after a wash-out of 1.5 h with drug-free solution. A second effect of the drug, the fast reinhibition, could be completely removed by wash-out. In summary i f is inhibited by ZD 7288 at membrane potentials at which the virtual i f gate is closed. Disinhibition occurs during long-lasting hyperpolarization but will hardly be operative in unclamped fibres under physiological conditions.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Monatsschrift Kinderheilkunde 145 (1997), S. 798-801 
    ISSN: 1433-0474
    Schlagwort(e): Schlüsselwörter Persistierender Ductus arteriosus (PDA) ; Katheterinterventioneller PDA-Verschluß ; Raskind-occluder ; Key words Patent ductus arteriosus (PDA) ; Transcatheter closure of PDA ; Rashkind occluder ; Front-loading-technique
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary Transcatheter closure of patent ductus arteriosus Botalli (PDA) has become a routine procedure. Only PDA in low birth weight infants, and short and wide open PDA in small children could not be closed by means of catheter technology. Different transcatheter closure systems are available now. We report on a premature infant now 16 month old weighting 6,8 kg were surgery seemed inadvisable. After angiography a funnel- shaped PDA seemed to be suitable for closure by a Ductocclud spiral coil (PFM company). The spiral coil could not be set in place safely because meanwhile the pulmonary PDA month had widened after angiography. Transcatheter closure then was successful using a 17 mm Rashkind double umbrella (Bard company) in modified implantation technique with a 8 french introduction set, and special attention to avoid the isthmus of the aorta and pulmonary artery stenosis. Discussion: Ductal reactivity at an age of 16 months seems unusual. Even at this age one has to be aware of possibly dramatic changes of the shape of the ductus during examination. Experience with several transcatheter PDA-closure systems is necessary for the successful closure even in cases of unexpected changes of shape of PDA.
    Notizen: Zusammenfassung Der katheterinterventionelle Verschluß eines persistierenden Ductus arteriosus Botalli (PDA) ist heute eine Routinemethode, für die eine ganze Reihe von verschiedenen Verschlußsystemen zur Verfügung steht. Eine Ausnahme für die Intervention stellen nur noch Frühgeborene und kleine Kinder mit kurzen, weit offenen Duktus dar. Wir berichten über ein 16 Monate altes, 6,8 kg schweres ehemaliges Frühgeborenes mit relativen Kontraindikationen zu einer Operation, bei dem nach Angiographie ein trichterförmiger Duktus zum Verschluß durch eine Ductocclud-Spirale (Fa. PFM) geeignet erschien. Die Spirale war jedoch nicht sicher zu plazieren, da sich die pulmonale Duktusmündung durch die mit der Intervention verbundenen Manipulationen zwischenzeitlich erheblich geweitet hatte. Der Verschluß des PDA gelang dann mit einem 17-mm-Rashkind-Doppelschirm (Fa. Bard) in modifizierter Implantationstechnik mit einer 8-Fr.-Schleuse unter besonderer Berücksichtigung der Schonung der Aortenisthmusregion und der Pulmonalarterienstrombahn. Diskussion: Die Empfindlichkeit des Duktusgewebes noch im Alter von 16 Monaten erscheint ungewöhnlich. Der Untersucher muß auch in diesem Alter auf drastische Änderungen der Duktusmorphologie vorbereitet sein. Bei entsprechender Erfahrung mit verschiedenen Verschlußsystemen kann die Intervention trotz „Duktusspiel” gelingen.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Zeitschrift für Herz-, Thorax- und Gefässchirurgie 12 (1998), S. 189-192 
    ISSN: 0930-9225
    Schlagwort(e): Schlüsselwörter Echokardiographie – Chirurgie angeborener Herzfehler ; Key words Echocardiography – Surgery of congenital heart disease
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary Purpose of this study was to evaluate the clinical utility of intraoperative echocardiography in the assessment of surgical repair of congenitally malformed hearts. In 235 of 508 patients who underwent cardiac surgery during a one year period transesophageal intraoperative echocardiography was performed. This was done to exclude residual ventricular septal defects or right ventricular outflow tract obstruction (n=84), to evaluate results of AV valve reconstruction (n=61), to assess ventricular function following arterial switch operation for complete transposition or in complex heart defects palliated by a Fontan-type procedure (n=50), to assess repair of left ventricular outflow obstruction (n=22) and for miscellaneous defects (n=18). Residual defects leading to immediate reoperation during the same surgical session were found in 4 patients (1.7%), minor residual defects not requiring reoperation were present in 6 patients (2.6%). In 2 patients with complete AV septal defect the left AV valves were only mildly regurgitant immediately after reconstruction but subsequent suture deshiscence required later reopoeration. Echocardiographic data were supplemented by direct pressure measurements in the ventricles and contrast injection into the left atrium. We conclude that intraoperative echocardiography can provide useful clinical informations, if its findings are interpreted in context with pressure measurements and contrast injections.
    Notizen: Zusammenfassung Zweck dieser Studie war, den klinischen Nutzen der intraoperativen Echokardiographie bei Patienten mit angeborenen Herzfehlern zu untersuchen. Von 508 während des Zeitraumes eines Jahres durchgeführten Operationen bei angeborenen Herzfehlern wurde bei 235 (47%) eine intraoperative Echokardiographie durchgeführt. Die Indikationen waren Ausschluß von residuellen Ventrikelseptumdefekten und residuellen rechtsventrikulären Ausflußbahnobstruktionen (n=84), Evaluation von AV Klappenrekonstruktionen (n=61), vorwiegende Evaluation der Ventrikelfunktion nach arterieller Switchoperation bei kompletter Transposition oder bei Palliation komplexer Herzfehler durch eine Fontanoperation (n=50), Untersuchungen nach Operation einer linksventrikulären Ausflußbahnobstruktion (n=22), sowie sonstige Operationen (n=18). Residuelle Defekte, welche zu sofortiger Reoperation in der gleichen Sitzung Anlaß gaben, wurden bei 4 Patienten (1,7%) gefunden, unbedeutende residuelle Defekte, die keine Reoperation erforderlich machten bei 6 Patienten (2,6%). In 2 Fällen wurden bei korrigierten kompletten AV Septumdefekten unmittelbar postoperativ geringe Insuffizienzen der linken AV Klappe gesehen, welche später so an Schweregrad zunahmen, daß spätere Reoperationen notwendig wurden. Die durch die transösophageale Echokardiographie erhobenen Befunde wurden durch direkte blutige Druckmessungen und Kontrastinjektion in den linken Vorhof ergänzt. Wir schlußfolgern, daß die intraoperative Echokardiographie klinisch sinnvolle Informationen liefern kann, wenn die erhobenen Befunde im Kontext mit den oben aufgeführten anderen Messungen interpretiert werden.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Zeitschrift für Herz-, Thorax- und Gefässchirurgie 12 (1998), S. 221-225 
    ISSN: 0930-9225
    Schlagwort(e): Schlüsselwörter Vorhofseptumdefekt – Interventioneller Verschluß– Echokardiographie ; Key words Atrial septal defect – transcatheter closure – echocardiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary Surgical closure of atrial septal defects can nowadays be performed without preoperative cardiac catheterization. For a transcatheter closure, however, x-ray exposure has been indispensable. We report a closure of an atrial septal defect under transesophageal echocardiography without fluoroscopy. A nine year old girl with an 11 mm atrial septal defect centrally located in the oval fossa was elected for transcatheter closure. Doppler-echocardiographically estimated Qp/Qs was 1.7:1. Oxymetric Qp/Qs was 1.6:1. After sedation with midazolam and propofol a diagnostic and interventional catheterization was performed without fluoroscopy. Under transesophageal echocardiography, the defect was sized over the wire with a 5F balloon catheter. The distance to the right pulmonary veins was 10 mm, to the coronary sinus and to the mitral valve 8 mm, respectively. Under transesophageal echocardiography, an 11 mm Amplatzer Septal Occluder was placed into the defect. Complete closure was achieved and no complications were encountered. We conclude that in selected cases with an atrial septal defect located in the oval fossa and clear-cut echocardiographic findings, an Amplatzer Septal Occluder can be safely deployed under echocardiographic guidance alone.
    Notizen: Zusammenfassung Der operative Verschluß eines Vorhofseptumdefekts (ASD) kann heutzutage ohne präoperative Katheteruntersuchung durchgeführt werden. Für den interventionellen Verschluß unter Durchleuchtung ist jedoch eine Strahlenexposition unvermeidbar. Wir berichten über einen interventionellen ASD-Verschluß allein unter transösophagealer Ultraschallkontrolle. Ein neun Jahre altes Mädchen mit einem 11 mm großen ASD vom Secundum-Typ wurde zum interventionellen ASD-Verschluß vorgestellt. Das Shuntvolumen über den Defekt wurde Doppler-echokardiographisch mit Qp/Qs=1,7:1 und oxymetrisch mit Qp/Qs=1,6:1 bestimmt. Unter Sedierung mit Midazolam und Propofol wurde sowohl die Katheterdiagnostik als auch die Intervention ohne Durchleuchtung durchgeführt. Die Defektgröße wurde unter transösophagealer Echokardiographie mit einem 5F Ballonkatheter ermittelt: Der Defekt war 11 mm groß, der Abstand zu den rechten Lungenvenen betrug 10 mm, zum Koronarsinus und zur Mitralklappe jeweils 8 mm. Unter transösophagealer Echokardiographie konnte komplikationslos ein 11 mm großer Amplatzer Septal Occluder in den Defekt plaziert werden. Es bestand kein Restshunt. Der hier berichtete Fall demonstriert, daß die Technik des interventionellen ASD-Verschlusses inzwischen so ausgereift ist, daß sowohl Diagnostik, Sizing als auch Implantation eines transvenös eingebrachten Amplatzer Septal Occluders ganz ohne Röntgenstrahlung möglich ist.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Intensive care medicine 24 (1998), S. 1232-1233 
    ISSN: 1432-1238
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 357 (1998), S. 291-298 
    ISSN: 1432-1912
    Schlagwort(e): Key words Rat ventricular myocyte ; Voltage-clamp ; Transient outward current ; Action potential ; Tedisamil
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Tedisamil has been described as a selective inhibitor of a fast inactivating transient outward current (ito,f) in rat ventricular myocytes. Because recent reports demonstrated the existence of a second slowly inactivating transient component (ito,s) we investigated ito,s and differentiated the effects of tedisamil on both transient outward current components and their influence on action potential duration. Standard electrophysiological techniques were used for whole cell recordings at 24–26° C from enzymatically isolated myocytes. Inhibition of ito,f by tedisamil was the result of an acceleration of inactivation at positive test potentials with a concentration for halfmaximal inhibition (EC50) of 4–7 μmol/l, which is confirmatory to reports from other investigators. Our new results show that ito,s is more sensitive to tedisamil with an EC50 of 0.5 μmol/l. Furthermore the pattern of ito,s inhibition is different compared with ito,f, because inactivation of ito,s is not accelerated by tedisamil. Instead the amplitude of the steady state inactivation curve of ito,s is attenuated which indicates a reduction of maximally available current. Ito,s was evaluated by three different methods as time-dependently inactivating current (7.5 s test pulse duration), voltage-dependently inactivated current and tedisamil-sensitive current. All approaches yield similar inactivation curves. The potential for halfmaximal inactivation of ito,s lies about 35 mV more negative than that for ito,f and the slope factor (K = –23 mV) is different to that of ito,f (K = –3 mV). Effectiveness of tedisamil-induced modulation of ito,f and ito,s on action potential repolarization was tested. Action potentials stimulated at 0.5 Hz were not prolonged by 1 μmol/l tedisamil (dominant ito,s block) at a repolarization level of 0 mV but prolonged to about 120% of control at –70 mV. This indicates that ito,f was sufficient to guarantee a regular early repolarization whereas decrease of ito,s delayed the final repolarization. In conclusion, the observation that tedisamil inhibits ito,f and ito,s differently supports the hypothesis that the two ito-components are related to two different channel populations expressed in rat ventricular myocytes.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 356 (1997), S. 788-796 
    ISSN: 1432-1912
    Schlagwort(e): Key words 17β-Estradiol ; Action potential ; Transient ; outward currents ; Calcium current ; Rat ventricular ; myocyte
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract This study describes electrophysiological effects of estrogens in isolated male rat ventricular myocytes. According to the literature these cells do not express the nuclear estrogen receptor. Action potentials or membrane currents were recorded in the whole-cell configuration with standard techniques. Action potential durations (APD) measured at a level of 0 mV (APD 0) and –70 mV (APD –70) were prolonged by 17β-estradiol (0.5 Hz stimulation frequency, 24–26° C). Threshold concentration was 1 μmol/l. At the highest concentration used (30 μmol/l) no saturation of the response was reached and APD 0 was 162% and APD –70 was 230% of the respective control. The resting potential remained unaffected in most cells. The prolongation induced by 17β-estradiol developed rapidly and reached a steady state 10 min after start of hormone superfusion. Effects of estrogen were completely reversible during 10–15 min wash-out with hormone-free solution. The extent of prolongation (10 μmol/l 17β-estradiol) was frequency dependent. Expressed as percentage of the respective control APD 0 (or APD –70) was 115% (188%) at 0.05 Hz, 118% (163%) at 0.5 Hz and 99% (129%) at 5 Hz stimulation frequency. The response was stereoselective, because 30 μmol/l 17α-estradiol did not prolong action potentials (APD 0: 101%, APD –70: 104% of the respective control, 0.5 Hz stimulation frequency). The endogenous estrogens estrone and estriol were less effective than 17β-estradiol. With 30 μmol/l estrone (0.5 Hz stimulation frequency) APD 0 was 103% and ADP-70 148% of control and with 30 μmol/l estriol APD 0 was 135% and APD –70 137% of control. The prolongation of action potentials can be explained by inhibition of transient outward current which, in rat ventricle, is composed of fast (i to,f) and slowly (i to,s) inactivating components. At 30 μmol/l 17β-estradiol i to,f was reduced to 50% and i to,s to 43% of their maximal amplitudes. The voltage sensor of i to,f or i to,s was hardly affected. Additionally, 17β-estradiol decreased the calcium current (i Ca,L) to 76% (10 μmol/l) and 38% at 30 μmol/l. The inwardly rectifying potassium current (i K1) was reduced partly with 30 μmol/l 17β-estradiol and its amplitude was 72% of control at –90 mV (inward current flow) and 65% at –40 mV (outward current flow). These results show that 17β-estradiol is active in cardiac cells which do not express the nuclear estrogen receptor. The hormone exerts class III activity and reduces calcium inward current. These effects, however, occur in vitro with concentrations above the physiological level and therefore may be without significance in vivo.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1569-8041
    Schlagwort(e): autologous transplant ; lymphoma ; mantle cell ; peripheral stem cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Background: Despite improved detection of mantle cell lymphoma (MCL),results of its treatment with conventional therapies remain disappointing andthe survival rate poor. The role of high-dose chemotherapy has recently beeninvestigated but no potential benefit has been clearly established. We reporthere our experience with MCL patients treated with intensive chemotherapy andautologous stem cell transplantation (ASCT). Patients and methods: Of the 16 MCL patients who received high-dosechemotherapy and ASCT beginning in 1989, six were treated in first-line and10 in sensitive relapse. Twelve of 16 patients received regimens whichincluded total body irradiation. All patients received peripheral blood stemcells (PBSC) with the exception of one, who underwent bone marrowtransplantation. Results: Three patients died of toxic effects of treatment. Three monthsafter transplant, seven achieved complete responses (CR) and two partialresponses (PR), two were stable and two had progressed. With a medianfollow-up after transplant of 22 months, five of the six surviving patientswere without progression, and three were in CR. The median times forevent-free survival (EFS) and overall survival (OS) were, respectively, 249and 317 days. The expected three-year EFS and OS were 24%. The mediansurvival after diagnosis was only 29 months. None of the criteria appeared tobe significantly associated with a better outcome, but first-lineintensification and a short delay after initial diagnosis may be favorable. Conclusion: In this study we were not able to confirm the hypotheticalbenefit of high-dose chemotherapy and PBSC transplantation in mantle celllymphoma, even though this approach may be promising in a subgroup of patient.
    Materialart: Digitale Medien
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