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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 43 (1981), S. 451-454 
    ISSN: 1432-1106
    Keywords: Monocular deprivation ; Cortico-geniculate projections ; Visual cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 16 cats monocularly deprived from 2 to 3 weeks of age, we studied 53 striate cortical cells which were identified as projecting to the dorsal lateral geniculate nucleus (LGN) on the basis of antidromic activation from LGN and of histological localization within cortical layer VI. As in the normal cat, these cortico-geniculate cells could be classified as slow, intermediate or fast, according to their axonal conduction velocities. The sampling ratio of the slow cells (mostly unresponsive to visual stimuli) was much higher than normal. On the other hand, the ratio of the intermediate (one half were simple cells) and fast cells (all except one were complex cells) was significantly lower than the norm. Also, the average axonal conduction velocities of the complex and simple cells were significantly slower than normal. These results suggest that normal maturation of cortico-geniculate cells, particularly fast and intermediate ones, is retarded or arrested by monocular visual deprivation.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 44 (1981), S. 41-56 
    ISSN: 1432-1106
    Keywords: Eye movements ; Dark-rearing ; Cat ; Vestibulo-ocular reflex ; Optokinetic nystagmus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cats reared in total darkness to adulthood have abnormal eye movements. A spontaneous nystagmus is found in the dark before any visual experience. The eye movements evoked by vestibular or optokinetic stimulation are less effective at compensation than for a normal cat. The vestibuloocular reflex (VOR) has a low gain (around 0.3) and a frequency dependent phase relation. The efficiency of optokinetic nystagmus (OKN) is poorer than for a normal cat, except for downwards stimulus movement which is followed better than normal. OKN is poorest in response to a stimulus viewed monocularly moving in the nasal to temporal direction. Neither VOR nor OKN of a dark-reared cat recover in efficiency within 5 months of the animal being brought into the light. A normal cat put into the dark for 135 days shows none of these abnormalities except an occasional spontaneous nystagmus.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 42 (1981), S. 73-80 
    ISSN: 1432-1106
    Keywords: Superior colliculus ; Vestibulo-ocular responses ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Unilateral lesions of the superior colliculus were made in normal cats. Following the operation, animals exhibited a typical neglect for contralateral visual space and forced circling toward the ipsilateral side. Optokinetic nystagmus was decreased for a stimulus moving toward the ipsilateral side, particularly in the temporal-to-nasal direction when the eye contralateral to the lesion was stimulated alone. — 2. When tested in the dark, animals exhibited a strong imbalance of their vestibulo-ocular responses (VOR) to velocity steps or to sinusoidal oscillations. Rotation of the animal toward the ipsilateral side produced a VOR with a higher gain, and a shorter phase-lead than in pre-operative controls. VOR was decreased in the opposite direction to a smaller extent, however. The overall asymmetry between the two sides at the post-operative stage was about 40%. — 3. In two animals, spontaneous nystagmus was present in the dark with the fast phase toward the ipsilateral side. — 4. Visual suppression of VOR was abolished during ipsiversive rotation and was still present during contraversive rotation. — 5. The effects of unilateral colliculectomy on VOR were transient. Spontaneous nystagmus disappeared in 3 days. VOR asymmetry in the dark was no longer present after 2–3 weeks. Loss of visual VOR suppression persisted for a few more days. — 6. Superior colliculus exerts a tonic control on static and dynamic components of VOR. This control may mediate part of VOR visual modulation and provide a substitutive input for compensation of pathological VOR asymmetry.
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  • 4
    ISSN: 1432-0568
    Keywords: Thalamus ; Ultrastructure ; Cat ; Quantitative analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the ventrobasal complex (VB) of the cat thalamus was investigated in order to compare the data with those obtained in other thalamic nuclei. New data are described regarding the identification of Golgi II type neurons and several forms of axo-somatic synapses. By means of a quantitative analysis the number of different profiles, distribution of interprofile contacts, synaptic densities and degree of synaptic input of the two main cell-types were defined. The quantitative data obtained in the VB complex were compared with values for the lateral geniculate body (CGL), nucleus anterior ventralis (AV-non-sensory relay thalamic nucleus) and nucleus lateralis posterior-pulvinar complex (LP-PU-associative thalamic nucleus). The quantitative data reveal that: 1. The occurrence of RL terminals is equal in the two relay nuclei (VB and AV) and three-fold higher than in the LP-PU. 2. The percentage ratio of specific terminals (RL) in the total surface area in the relay nuclei is three-fold higher than in the LP-PU. 3. The RL→D synapses are twice as numerous in the relay nuclei as in the associative nucleus. 4. The density of synaptic RS profiles in VB is half of that found in LP-Pu. 5. In the thalamic relay nuclei the number of synaptic contacts on Golgi II type neurons is one-third of the synaptic supply of relay neurons; in the LP-Pu the amount of synaptic contacts on Golgi II type neuron approaches the synaptic supply of projective neurons.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 41 (1981), S. 233-246 
    ISSN: 1432-1106
    Keywords: Cat ; Ventral tegmental area ; Locus ceruleus ; Parabrachial nuclei ; Horseradish peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Thirty-three cat brains with injections of horseradish peroxidase in various regions of the cerebral cortex were screened for afferent projections from the ventral tegmental area, the locus ceruleus, and the parabrachial nuclei. All three structures were found to project to rather divergent parts of the cortex, including regions in the posterior half of the hemisphere. These results, especially for the ventral tegmental area and, to a lesser degree, for the parabrachial neurons, disagree with most of the target loci of established cortical afferents in the rat. Though our results might be attributed to species differences in the cortical innervation of brain stem structures, we prefer explanations which emphasize different densities in the distribution of brain stem afferents to the cortex, and/or which suggest different cortical targets of catecholaminergic and noncatecholaminergic neurons.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1106
    Keywords: Vestibulo-ocular reflex ; Optokinetic nystagmus ; Vestibular habituation ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1.Unilateral habituation of the vestibuloocular reflex was produced in adult cats stimulated by repeated unidirectional velocity steps (vestibular training) or by a continuously moving visual surround (optokinetic training). — 2. Unidirectional vestibular training produced a strong asymmetry of vestibuloocular responses (VOR). Responses to velocity steps applied to the “trained” labyrinth were decreased both in gain and in time-constant. This effect generalized to responses to sinusoidal oscillations (0.03 Hz to 0.1 Hz), i.e. to a stimulus not used during training. — No spontaneous nystagmus was ever observed in spite of the dynamic VOR asymmetry. — 3. Unilateral vestibular habituation produced by vestibular training appeared to be a long-lasting phenomenon. It was still present 10 days after the end of training. — 4. Optokinetic responses were not affected by vestibular training. — 5. Unidirectional optokinetic training produced an increase in the slow phase velocity of optokinetic nystagmus (OKN) by about 25% in both directions. This effect did not persist for more than a few minutes. A marked spontaneous nystagmus was recorded in the dark after each session of optokinetic training, with a slow phase in the direction opposite to the previous OKN. — 6. VOR in response to velocity steps and to sinusoidal oscillations were decreased unilaterally after optokinetic training. This effect was of short duration, however, and disappeared within the interval between training sessions. This lack of retention contrasted with the prolonged effect of vestibular training.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 42 (1981), S. 442-452 
    ISSN: 1432-1106
    Keywords: Chemoreceptors ; C Fibers ; Vagal nerves ; Gastro-duodenal Region ; Microelectrode technique ; Cat ; Chémorécepteurs ; Fibres C ; Nerfs vagues ; Région Gastro-duodénale ; Technique des ; Microélectrodes ; Chat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé La sensibilité vagale aux hydrates de carbone (glucose en particulier) et aux acides (acide chlorhydrique et acide acétique) a été étudiée dans la région gastro-duodénale chez des chats anesthésiés. Les potentiels d'action ont été enregistrés dans le ganglion plexiforme droit au moyen de microélectrodes extracellulaires. Des récepteurs, sélectivement sensibles soit aux hydrates de carbone, soit aux acides, ont été ainsi mis en évidence. Ces récepteurs doivent donc être considérés comme de véritables glucorécepteurs et acidorécepteurs. Les deux types de chémorécepteurs, silencieux au départ, présentent une fréquence de décharge basse qui varie entre 2 et 14 imp/s pour les acido-récepteurs et entre 2 et 30 imp/s pour les glucorécepteurs. Les réponses peuvent être irrégulières ou régulières et durer plusieurs minutes; leur latence est faible (entre 1 et 20 s) pour les deux types de chémorécepteurs, ce qui suggère une localisation dans la muqueuse ou au voisinage de celleci. Les fibres connectées aux glucorécepteurs et aux acido-récepteurs ont une vitesse de conduction qui varie entre 0,8 et 1,2 m/s: elles sont donc amyéliniques. L'enregistrement de l'activité électromyographique de la région gastro-duodénale a permis de montrer que les acido-récepteurs et les glucorécepteurs interviennent dans la régulation de la motilité gastrique et duodénale, l'effet le plus marqué étant une diminution du rythme électrique de base de l'estomac.
    Notes: Summary Vagal sensitivity to carbohydrates (mainly glucose) and to acids (hydrochloric and acetic acids) was studied in the gastro-duodenal region of anesthetized cats. Action potentials were recorded extracellularly from the nodose ganglion by means of glass microelectrodes. Receptors responding to glucose perfusion were found at this level as well as receptors stimulated by acid perfusion. It is shown that each type of receptor was activated by only one kind of stimulus (carbohydrates and acids, respectively). These receptors must thus be considered as true glucoreceptors or acido-receptors. Being silent before activation, these two types of chemoreceptors discharged at a frequency that varied from 2 to 14 imp/s for the acido-receptors, and from 2 to 30 imp/s for the glucoreceptors. The response could be irregular or regular and might last several minutes. Its latency was short (between 1 and 20 s for both types of chemoreceptors. It was therefore supposed that they were located in the mucosa or neighboring structures. From the conduction velocities (0.8–1.2 m/s), it was concluded that fibers originating from the acidoand glucoreceptors belonged to the C type. Recording of the electromyographic activity from the gastro-duodenal region demonstrated that the gluco- and acido-receptors are involved in the regulation of motility of the gastric and duodenal regions.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 43 (1981), S. 25-33 
    ISSN: 1432-1106
    Keywords: Cat ; LP-pulvinar complex ; MIN ; Visual texture ; Receptive field properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Multiple visual field representations are contained within the feline LP-pulvinar complex; regions differentiated by their afferent and efferent connectivity patterns as the striate-, tecto- and retino-recipient zones. Cell responses from these visuotopic zones were investigated in immobilized cats under N2O/O2 supplemented with pentobarbitone or Althesin, using spot, bar and textured stimuli. Response fields recorded within the LP-pulvinar complex were classified as diffuse, concentric, movement-, direction- or orientation-sensitive. Concentric receptive fields were further classified as sustained (X), transient (Y) or tonic/phasic W-cells. Direction-and movement-sensitive cells predominated in the striate- and tecto-recipient zones, respectively. Motion of noise fields, or noise bars against an identical stationary noise background elicited vigorous responses from cells in the striate zone, many showing a preference for noise stimuli. In contrast, cells from the tectal zone and other divisions of the LP-pulvinar complex were insensitive to noise. The retino-recipient zone at the lateral margin of the pulvinar nucleus was characterized by cells with concentric receptive fields, the majority exhibiting properties similar to W-cells in the LGNd. The evidence supports the notion of functional subdivision within the LP-pulvinar complex corresponding to the visuotopically organized regions defined by their connectivity patterns. Consideration of the retino-recipient zone as an extension of the LGNd-MIN complex is discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 43 (1981), S. 101-103 
    ISSN: 1432-1106
    Keywords: Cat ; Pyramidal tract ; Corticospinal system ; Locomotion ; Stepping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We studied the pattern of stepping in cats, before and after the placement of lesions in the corticospinal system. We found a deficit after the lesions, which was characterized by increased extension of the involved hindlimbs during the stance (E2–E3) phase of the step cycle. This deficit lasted two weeks or less. It may reflect loss of inhibitory influences upon extensor motoneuron pools.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 43 (1981), S. 413-418 
    ISSN: 1432-1106
    Keywords: Gracile nucleus ; Plasticity ; Somatotopy ; Agenetic defect ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The somatotopic representation of the hindlimb in the gracile nucleus was studied in two cats who had a congenital defect of one hindfoot. The defect comprised all of the foot downward from and including the heel, and the distal third of tibia and fibula. The part of the sciatic nerve normally supplying the lower hindlimb and the hindfoot was reduced in diameter by one third. The motoneurones corresponding to the absent muscles were lacking and replaced by glial elements. The cross-sectional area of the dorsal columns at segment S2 was reduced by more than 20%. The gracile nuclei, in contrast, were not reduced in size. Only the diameter of its neurones was significantly smaller. Electrophysiological single and multi-neurone recordings revealed an altered somatotopic representation in the gracile nucleus on the defective side. The nuclear area normally representing the missing parts of the body surface now received input from the stump. There was no nuclear area devoid of afferent input, and there was no input in the gracile from the forelimb or from the contralateral side. It is concluded that the remaining parts of the leg project onto the gracile nucleus in an ordered fashion, sharing the entire nucleus according to their present afferent fibres.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 43 (1981), S. 422-428 
    ISSN: 1432-1106
    Keywords: Retinal ganglion cells ; Cat ; Rod and cone mediated receptive fields ; Receptive field size/shape
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Receptive field centres of cat retinal ganglion cells, as mediated by rod and by cone inputs, were mapped as contours of iso-sensitivity at a mid-mesopic adapting luminance using, respec-tively, 452 nm-blue and 578 nm-yellow narrow-band lights at an intensity 1 log unit above threshold for the most sensitive locus. Based on the sizes and shapes of mapped rod and cone centres for 74 ganglion cells, four receptive field centre categories were distinguished. Cone and rod centres were usually elliptical, and in almost 60% of cells the major axis through the receptive field centre was oriented within ±20 ° of horizontal. In 69%, rod and cone centres were non-concentric, 66% had larger rod than cone centres — area ratios ranging from 0.6 ∶ 1 to 2.9 ∶ 1, and in only two cases was the rod centre actually smaller than the cone centre.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 41 (1981), S. 301-309 
    ISSN: 1432-1106
    Keywords: Cat ; Auditory ; Localization ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Responses of single neurones in the inferior colliculus of anaesthetized adult cats and kittens were studied using best-frequency stimuli of varying interaural intensity difference (IID). Two broad classes of neurone, distinguished by the predominant type of input from each ear, were examined. One class of cells received predominantly excitatory input from each ear (EE cells). The other class were excited by monaural stimulation of the contralateral ear and showed no response to monaural stimulation of the ipsilateral ear, but inhibition of the excitatory response by simultaneous ipsilateral stimulation (EI cells). Fourteen of the 18 adult EI cells showed marked changes in discharge rate with variation in IID. Adult EI cells showed low response variability and were insensitive to changes in average binaural intensity. In all cases of IID sensitivity, the onset component of the response was less sensitive to IID than the sustained component. Eight out of ten EE cells were insensitive to IID over the range tested. Cells of high best-frequency in kittens younger than 28 days showed irregular changes in discharge rate with variation in IID and wide response variability. Some low-frequency EI cells in young kittens showed sensitivity to IID, but it is unlikely that these could be involved in sound localization as their frequency response was inappropriate. Many cells in kittens aged 31–40 days showed monotonic, adult-like IID functions, but the response variability of these cells remained higher than that of adult cat neurones. These data provide evidence for a developmental change of binaural interaction in the cat.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 41 (1981), S. 329-337 
    ISSN: 1432-1106
    Keywords: Caudate nucleus ; Golgi/EM ; Corticocaudate projection ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A combined Golgi/electron microscopic technique was used to investigate the fine structure and synaptology of Golgi-stained spiny neurons in the caudate nucleus of the cat. In order to study the termination sites of cortical afferents on Golgistained spiny neurons, cortical fibres were caused to degenerate by making extensive cortical lesions 3 days prior to fixation of the animals. When examined in the electron microscope, perikarya of labelled spiny neurons have a round nucleus, a few mitochondria and microtubules, and a poorly developed Golgi apparatus and rough endoplasmic reticulum. Only rarely are axo-somatic contacts seen. Labelled dendrites exhibit a moderate number of microtubules and sometimes elongated mitochondria. Numerous labelled spines are seen in the vicinity of their parent dendrites. They are contacted by smaller type I and type III boutons and larger type IV boutons (Hassler et al. 1978). Large boutons filled with clear round vesicles establish symmetric contacts with labelled dendritic shafts. Degenerating boutons of cortical afferents are seen in contact with spines and, more rarely, with dendritic shafts of Golgi-stained spiny neurons. All degenerating boutons synapsing with labelled structures are found some distance from the cell body. No contacts of degenerating cortical boutons with the soma or with stem dendrites of Golgi-stained spiny neurons are found.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 42 (1981), S. 196-202 
    ISSN: 1432-1106
    Keywords: Retina ; Sampling theorem ; Brisk, X and Y, ganglion cells ; Cat ; Visual acuity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relationship of brisk transient and brisk-sustained unit cut-off frequencies to their respective array cut-off frequencies has been calculated by means of sampling theory from the corresponding α and β-mode cell densities at various retinal eccentricities. Interpretation of the results depends on whether on and off cells of each class are functionally homogeneous or heterogeneous populations. In agreement with expectations from sampling theory for a system involved in resolution tasks, it is found that the brisk-sustained system has less potential for undersampling and spurious imagery than the brisk-transient system, which is thought to play a different role. The brisk-sustained array could avoid such aliasing altogether if it is functionally heterogeneous. The differences between the two systems are similar to those between insect eyes respectively optimised for high resolution vision or the detection of high velocity image motion.
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  • 15
    ISSN: 1432-1106
    Keywords: Oculomotricity ; Lateral rectus and retractor bulbi motoneurones ; Infraorbital nerve ; Vibrissal pad ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Responses from lateral rectus, medial rectus and retractor bulbi nerves were obtained following electrical stimulation of the vibrissal pad of the cat. Discharges in afferent fibres dissected from the infraorbital nerve were recorded during movements of the vibrissae and following electrical stimulation of the vibrissal pad. Both stimuli activated the same population of Aα fibers. Intracellular records were obtained from lateral rectus motoneurones identified antidromically in the principal abducens nucleus and from retractor bulbi motoneurones similarly identified in the accessory abducens nucleus. EPSPs (3 mV) were recorded in lateral rectus motoneurones following electrical stimulation of the ipsilateral vibrissal pad at a latency of 3.5 ms. Large-amplitude disynaptic EPSPs (15 mV) were recorded in retractor bulbi motoneurones following the same vibrissal stimulation. The synaptic excitation evoked in both lateral rectus and retractor bulbi motoneurones through stimulation of the ipsilateral vibrissal pad induced an early retraction followed by an abduction of the eye ball. The hypothesis is that the vibrissal message might complement other sensory modalities in the generation of patterned eye movements.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 44 (1981), S. 431-436 
    ISSN: 1432-1106
    Keywords: Cat ; Visual cortex ; Orientation columns ; Deoxyglucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Three-dimensional reconstructions of the orientation column system were obtained from the visual cortex of four cats using the deoxyglucose technique. One cat had normal visual experience, one was monocularly deprived and two had selective experience with vertical and horizontal contours, respectively. In areas 17 and 18 orientation columns form a remarkably regular system of equally spaced parallel bands whose trajectory is orthogonal to the borderline between areas 17 and 18. This topographic organization is resistant to manipulations of early visual experience.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 44 (1981), S. 437-440 
    ISSN: 1432-1106
    Keywords: Response variability ; Visual cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relationship between the variance and mean rate of discharges of simple cells in the cat striate cortex has been examined when mean rate was varied by changing either stimulus spatial frequency or contrast. In both cases, the variance was related to the mean discharge rate by an exponent of about 1.15; the relation was thus roughly linear. The discharge variance was on average 1.7 times the mean rate for data obtained from measurements of the neurones' spatial frequency tuning curves, and 1.48 times the mean for data from the response-contrast determination. However, this difference was not statistically significant.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 41 (1981), S. 420-426 
    ISSN: 1432-1106
    Keywords: Vestibular nystagmus ; Cat ; Visual suppression ; Vertical asymmetries ; Nystagmus vestibulaire ; Chat ; Suppression visuelle ; Asymétries verticales
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Chez le chat, l'asymétrie des réponses nystagmiques verticales à une rotation autour d'un axe interaural a été caractérisée en mesurant la fréquence de battement et le gain du réflexe vestibulooculaire dans chaque sens vers le haut et vers le bas. Des variations sinusoïdales de la vitesse de la tête, ou des échelons de vitesse ont été exercés dans trois conditions visuelles: à l'obscurité (stimulation vestibulaire pure); à la lumière (stimulation mixte vestibulaire et optocinétique); et enfin, alors qu'un miroir placé devant l'animal et solidaire du mouvement de sa tête lui fournissait une référence visuelle stable (vision stabilisée). Dans les trois conditions, la fréquence de battement et le gain du nystagmus vers le bas (désigné par le sens des phases rapides) sont plus grands que ceux du nystagmus vers le haut. A l'obscurité, les caractéristiques des nystagmus postrotatoires suggèrent une constante de temps plus longue pour le réflexe vestibulo-oculaire vers le bas que vers le haut. A la lumière, les deux stimulations agissent synergiquement. En vision stabilisée, la suppression du nystagmus s'exerce préférentiellement sur le nystagmus haut, ce qui appuie l'hypothèse d'une addition algébrique des effets des deux stimulations.
    Notes: Summary In the cat, the asymmetry of vertical nystagmus in response to a rotation around the Yaxis has been characterized by measuring the beat frequency and gain of vestibulo-ocular reflexes in each direction (upward and downward). Sinusoidal variations of head velocity or velocity steps have been applied under three visual conditions (a) in darkness (pure vestibular stimulation); (b) in the light (mixed vestibular and optokinetic stimulation); (c) with a mirror placed in front of the animal; since the mirror image moved with the head, the animal was provided with a stable visual cue (stabilized vision). In all three conditions, beat frequency and gain were greater for downward than for upward nystagmus (the direction refers to that of the quick phase). In darkness, the characteristics of postrotatory nystagmus suggested a greater time constant for downward than for upward vestibulo-ocular reflexes. In the light, both stimuli acted synergistically. In stabilized vision, upward vestibular nystagmus was preferentially suppressed, suggesting an algebraic summation of the effects arising from both kinds of stimuli.
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 42 (1981), S. 89-98 
    ISSN: 1432-1106
    Keywords: Receptive field ; Single unit ; Striate cortex ; Simple cell ; Cat ; Model stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The receptive field organization of simple cells was studied by analyzing interaction effects between two stationary flashing light stimuli. One stimulus was positioned in the most responsive part of the receptive field to produce activity against which the effects of the other stimulus in various positions of the visual field could be determined. The receptive field was subdivided into an elongated center and elongated antagonistic flanks. The effects on the flanks were always considerably stronger on one side. Powerful flank suppression could be elicited within a region which usually was only slightly wider than the receptive field center. The suppression was just as stimulus specific as the activation of the center and occurred only by light ON or OFF. The cells were classified into ON-dominant and OFF-dominant depending on the kind of response found in the center. In ON-dominant cells the strong flank suppression occurred only by light ON, and light OFF produced enhancement. Correspondingly, the strong flank suppression occurred only by light OFF in OFF-dominant cells. This is consistent with the interpretation that simple cells have excitatory and inhibitory input from the same type of cells in the lateral geniculate nucleus (LGN), i.e., only from ON-center or OFF-center cells. The small size of the area where strong flank suppression occurred shows that inhibition comes from a few LGN cells rather than from a large pool of cells. A model for simple cell receptive fields presuming overlapping but acentric excitatory and inhibitory fields with input to both fields from either ON- or OFF-center LGN cells was tested by computer simulation and shown to fit the experimental data.
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 42 (1981), S. 99-107 
    ISSN: 1432-1106
    Keywords: Receptive field ; Single unit ; Striate cortex ; Complex cells ; Cat ; Model simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The receptive field organization of complex cells was studied by analyzing interaction effects between two stationary flashing light stimuli. One was placed in the most responsive part of the receptive field to produce activity against which effects of the other in different visual field positions could be determined. The receptive field was spatially organized into antagonistic center and flanks just like the fields of simple cells. However, both center and flanks were found within the receptive field area where a single slit evoked discharge. Center and flanks were elongated along the optimal stimulus orientation. The flanks were displaced from the center normal to optimal stimulus orientation. In the center, ON- and OFF-responses were usually about equal in strength and the maximum ON- and OFF-responses occurred in about the same position. This shows that complex cells are activated by input from both ON- and OFF-center cells in the lateral geniculate nucleus (LGN) where the receptive field centers of the LGN cells overlap closely. This explains most of the specific features of complex cells, e.g., the spatially overlapping ON- and OFF-zones, the large response field, the repetitive firing when a slit moves over the receptive field, and the marked non-linear spatial summation. Strong flank suppression occurred with both ON and OFF. The effects were usually stronger on one side of the center. Maximal suppression occurred on the same side with both ON and OFF. This is consistent with the interpretation that complex cells are inhibited by input from both LGN ON- and OFF-center cells with overlapping receptive field centers. A model presuming that complex cells have overlapping but acentric excitatory and inhibitory fields was tested by computer simulation and shown to fit the experimental data. This is the same model as presented for simple cells in the preceding paper (Heggelund 1980), except that the excitatory and inhibitory fields of simple cells have input from either ON- or OFF-center LGN cells, whereas in complex cells they have input from both types.
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  • 21
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    Experimental brain research 42 (1981), S. 146-157 
    ISSN: 1432-1106
    Keywords: Nucleus tractus opticus ; Visual response ; Direction specificity ; Optokinetic nystagmus ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary All cells in the nucleus of the optic tract (NOT) of the cat, that Bcould be activated antidromically from the inferior olive, were shown to be direction-specific, as influenced by horizontal movements of an extensive visual stimulus. Cells in the left NOT were activated by leftward and inhibited by rightward movement, while those in the right NOT were activated by rightward and inhibited by leftward movement. Vertical movements did not modulate the spontaneous activity of the cells. The mean spontaneous discharge rate in 50 NOT cells was 30 spikes/s. This direction-specific response was maintained over a broad velocity range (〈0.1 ° – 〉100 °/s). Velocities over 200 °/s could inhibit NOT cells regardless of stimulus direction. All cells in the NOT were driven by the contralateral eye, about half of them by the ipsilateral eye also. In addition, activation through the contralateral eye was stronger in most binocular units. Binocular cells preferred the same direction in the visual space through both eyes. An area approximately corresponding to the visual streak in the cat's retina projected most densely onto NOT cells. This included an extensive ipsilateral projection. No clear retinotopic order was seen. The most sensitive zone in the very large receptive fields (most diameters being 〉20 °) was along the horizontal zero meridian of the visual field. The retinal input to NOT cells was mediated by W-fibers. The striking similarities between the input characteristics of NOT-cells and optokinetic nystagmus are discussed. The direction selectivity and ocular dominance of the NOT system as a whole can provide a possible explanation for the directional asymmetry in the cat's optokinetic nystagmus when only one eye is stimulated.
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  • 22
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    Experimental brain research 44 (1981), S. 177-186 
    ISSN: 1432-1106
    Keywords: Posture ; Movement ; Motor cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of postural support on posturo-kinetic co-ordination has been studied with a model of movement consisting of limb flexion induced by stimulation of the motor cortex in chronically implanted cats. To more easily quantify the responses by measuring the vertical forces exerted at the level of the four limbs, only weak stimuli were used, mainly triggering isometric motor activity. The aim of the study was to analyse the influence on the motor responses of various postural supports leading to a weight distribution between the limbs which differs from the normal situation. Three kinds of initial posture were tested. The first was obtained by directing the head of the cat to the left or to the right. The second type consisted in raising one or two of the platforms supporting the limbs. The third was obtained by adding a load to one of the limbs. The main results established in these conditions were the following: The latencies of the beginning of the movement and its associated postural adjustment, as revealed by the onset of force changes, were independent of the initial postural situation. Whatever the initial weight distribution, the amplitudes of force variations induced by given intensities of cortical stimulation were generally constant, though small variations of amplitude were noted in some cases. When there were changes in the response amplitudes, all four limbs were generally affected. The effect was observed whatever the limb concerned by modification of the support. This not only means that the postural responses depend on the amplitude of the movement but also that the amplitude of the movement itself may be modified according to perturbations affecting the postural limbs. Comparison of the present data with previous results leads to the hypothesis that a postural reference is taken into account in the determination of amplitudes of responses to cortical stimulation.
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  • 23
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    Pflügers Archiv 391 (1981), S. 226-230 
    ISSN: 1432-2013
    Keywords: Central chemosensitivity ; Morphology ; HRP ; Labelling ; Mapping ; Medulla oblongata ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A modified horseradish peroxidase labelling technique was used to study the distribution pattern of neurons in the central chemosensitive fields of the medulla oblongata of cats. In several cryosectioned medullae a mapping of superficially located HRP-labelled neurons was achieved. The distribution and configuration of the labelled neurons indicate that most of them belong to the nucleus paragigantocellularis lateralis. However, by varying the time of incubation it was possible to identify different types of neurons. On the basis of certain aspects of the HRP incorporation mode and neuronal topography a specific type of small-sized neurons has been identified. Some functional implications of these small neurons with respect to their possible chemosensitive activity are discussed.
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  • 24
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    Pflügers Archiv 389 (1981), S. 143-148 
    ISSN: 1432-2013
    Keywords: Retractor bulbi muscle ; Trigeminal afferents ; Oculomotricity ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of vestibular and trigeminal stimulation on reflex responses of each slip of the retractor bulbi muscle were tested by recording the electromyogram. 1. In “encéphale isolé” cat, phasic electrical stimulation of the horizontal canal induced no response in the RB slips. Repetitive vestibular stimulation did not produce nystagmus in the RB muscle while strong muscular discharges were observed in the nystagmus lateral rectus muscle. 2. In anaesthetized cats, three trigeminal inputs elicited strong reflex responses in each slip of the RB muscle. Electrical stimulation of the vibrissae or the infra-orbital nerve evoked a two component reflex response (latencies: 5ms±0.5 and 14ms±2). Electrical stimulation of the supraorbital nerve elicited a single component reflex response (latency: 6ms±0.5). Electrical stimulation of the long ciliary nerves evoked a complex response with four components (latencies: 7.5ms±0.5, 10ms±2,15ms±2,20ms±2) 3. Pentobarbital and morphine produced lasting depression of the reflex responses of the RB muscle. The depressive effect of morphine was reversed by naloxone.
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  • 25
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    Naunyn-Schmiedeberg's archives of pharmacology 317 (1981), S. 168-172 
    ISSN: 1432-1912
    Keywords: Paraoxon ; Blood pressure ; Vertebral artery ; Central muscarinic receptors ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Application of paraoxon into the left vertebral artery (8–80 μg) or both the left and right vertebral artery (4–8 μg) of the anaesthetized cat evoked dose-dependent depressor effects, whereas heart rate was not influenced significantly. Also after systemic administration of paraoxon (150–825 μg·kg−1), while peripheral muscarinic receptors were blocked, depressor effects were still observed. Dose-response curves for the depressor response to paraoxon were established. Infusion of low doses of dexetimide via the vertebral artery prevented the hypotensive action of paraoxon. The distribution of this antimuscarinic drug in the brain was investigated. The depressor effect of paraoxon can be attributed to both a decrease in peripheral resistance and cardiac output. Decerebration and midcollicular transection were carried out in order to elucidate the site and mechanism of action. The depressor effect of paraoxon seems to be mediated by a central mechanism of action located within the lower brain stem. It is concluded that stimulation of muscarinic receptors in the pontomedullary region gives rise to the observed changes in haemodynamic parameters. Muscarinic receptors in the hypothalamus seem to be of minor importance for the hypotensive action of paraoxon.
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  • 26
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    Cell & tissue research 216 (1981), S. 557-568 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Neuroglia ; Silver impregnation ; Brain ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The silver-impregnation procedure of Tsujiyama is suitable for demonstration of all three classical types of neuroglial cells; in the present study it was used for electron microscopic identification of neuroglial cells in the brain of the cat. The aim of the present study was 1) to determine impregnated structural correlates of neuroglial cells at the light- and electron-microscopic levels, and 2) to determine whether the method of Tsujiyama is applicable for the electron microscopic identification of the single types of neuroglial cells. Silver deposits were observed over the cytoplasm and processes of astrocytes where numerous glial filaments were present. Oligodendrocytes and microglial cells may be precisely differentiated by use of Tsujiyama's silver impregnation method at the electron microscopic level due to the pattern of silver-deposition in these two basic types of cells. This silver-impregnation method combined with electron microscopy is thus suitable for a precise identification of neuroglial cells; the technique may prove to be very helpful in identification of such categories of neuroglial cells that encompass also the images of cells which cannot be classified by use of the standard methods.
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  • 27
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    European archives of oto-rhino-laryngology and head & neck 233 (1981), S. 1-12 
    ISSN: 1434-4726
    Keywords: CM ; SP ; Hirnstamm-Reaktion ; Katze ; Entwicklung ; CM ; SP ; Brainstem response ; Cat ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The development of the surface-recorded cochlear and early neural potentials has been studied in the cat from birth to the 8th post-natal week. CM, SP, and each of the neural waves has a characteristic temporal developmental pattern and mature threshold. Wave IV is the most resistant to reduction in stimulus intensity levels, while the thresholds of both cochlear potentials are high. Animals with hereditary unilateral deafness constitute a most convenient model for the study of functional maturation in the auditory pathways, particularly with regard to precise latency estimations.
    Notes: Zusammenfassung Die Entwicklung der an der Oberfläche registrierten cochlearen und frühen neuralen Potentiale wurde bei der Katze von der Geburt bis zur 8. Woche studiert. CM, SP und jeder der neuralen Wellen haben ein charakteristisches altersbedingtes Entwicklungsmuster und eine Responsschwelle. Welle IV wird am wenigsten von einer Reduktion in der Stimulusintensität beeinflußt. Die Schwelle der beiden cochlearen Potentiale sind dagegen erhöht. Tiere mit angeborener einseitiger Taubheit sind bestens geeignet für das Studium der funktionellen Reifung der Gehörbahnen, besonders in bezug auf die exakten Latenzzeitmessungen.
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    European archives of oto-rhino-laryngology and head & neck 230 (1981), S. 251-255 
    ISSN: 1434-4726
    Keywords: Ganglion Scarpae ; Katze ; Labyrinthektomie ; Scarpa's ganglion ; Cat ; Labyrinthectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary One year after membraneous labyrinthectomy in three cats a quantitative analysis of neurons in Scarpa's ganglion was performed under the light microscope. Total numbers of neurons were found 30% lower than in the respective contralateral ears. Although remaining perikarya showed signs of degeneration, such as shrinkage and vacuoles the proportions of large and small neurons remained unchanged after this procedure.
    Notes: Zusammenfassung Ein Jahr nach Labyrinthektomie wurde eine quantitative Analyse der Neuronen im Ganglion Scarpae der Katze durchgeführt. Die Gesamtzahl der Perikarya war 30% niedriger als in den unoperiert belassenen kontralateralen Ohren. Obwohl die verbleibenden Neuronen degenerative Veränderungen wie Schrumpfung und Vakuolen aufwiesen, waren die Anteile an großen und kleinen Neuronen unverändert.
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  • 29
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 11-16 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; SCOT column ; Coating, liquid phase ; Single-step coating ; Phenols ; Phenolic acids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A single-step coating method for the preparation of glass capillary SCOT columns is described. It is reproducible and less time-consuming than the well-known two-step coating procedures. Other methods attempted are discussed briefly. Both the flame ionization and electron capture detectors could be used in conjunction with temperature programming. The separations achieved with an “activity mixture”, phenols and phenolic acids, illustrate the resolution obtained. The columns are suitable for quantitative determinations and a comparison is made with a conventional packed column.
    Additional Material: 6 Ill.
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  • 30
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    Journal of High Resolution Chromatography 4 (1981), S. 35-36 
    ISSN: 0935-6304
    Keywords: Gel permeation chromatography, GPC ; High-speed analysis ; Pollen allergen extract ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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    Journal of High Resolution Chromatography 4 (1981), S. 115-120 
    ISSN: 0935-6304
    Keywords: TLC ; Gradient and continuous development ; Amino acids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Thin-layer gradient elution chromatography is a convenient technique for separation of very complicated mixtures; in some cases the gradient technique can be replaced by continuous development. The two techniques are compared in practice by using them to separate a mixture of dabsyl derivatives of amino acids.
    Additional Material: 6 Ill.
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  • 32
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    Journal of High Resolution Chromatography 4 (1981), S. 123-124 
    ISSN: 0935-6304
    Keywords: (GC)2 in short capillaries ; Liquid crystalline stationary phase ; Supercooled temperature range ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 33
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    Journal of High Resolution Chromatography 4 (1981), S. 302-304 
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; Hemagglutinin glycoprotein of influenza virus investigated ; Peptide separation ; 14C labeling for identification ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The potential of reverse-phase high-performance liquid chromatography (RP-HPLC) for the separation of the tryptic glycopeptides of the hemagglutinin (HA) glycoprotein of influenza virus has been investigated. Excellent separation was accomplished with sample recovery of at least 90-95% by the utilization of an octadecasilyl silica stationary phase and a phosphoric acid/n-propanol solvent system. Eight glycosylated tryptic peptide classes were resolved from the HA glycoprotein of the A/USSR/90/77 virus strain.
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  • 34
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    Journal of High Resolution Chromatography 4 (1981), S. 477-478 
    ISSN: 0935-6304
    Keywords: Square relationship ; Effect of separation efficiency ; Simple equation for plate comparison ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
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  • 35
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    Journal of High Resolution Chromatography 4 (1981), S. 42-42 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 36
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    Journal of High Resolution Chromatography 4 (1981), S. 141-142 
    ISSN: 0935-6304
    Keywords: TLC ; Reflectance ; Transmission ; Probability vector ; Transition matrix ; Markov chains ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Changes of reflected and transmitted light in cases where a band lies in different sublayers of a thin-layer chromatogram are calculated by the use of Markov chains. The problem of a “random walk with absorbing barriers” is described and compared with the problem of transmission and reflectance of light in a layer of sorbent.
    Additional Material: 1 Ill.
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  • 37
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    Journal of High Resolution Chromatography 4 (1981), S. 151-155 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; XAD-2 resin ; Fungicide residues, determination in wine and grape juice ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The fungicides Bupirimate, Fenarimol, and Vinclozolin were sprayed on Vines. The fruits were harvested 8 and 21 days after the final application and processed to juice and wine. The fungicide residues were determined by filtration of the samples throug XAD-2 after pH adjustment, elution with methylene chloride, and glass capillary gas chromatography with SE-30 as stationary phase. Residue values of the three compounds were reported before and after fermentation. The precision of the analytical method was established by spiking portuguese white wine and spanish grape juice with the three fungicides and Triadimefon. The recoveries were in the range 80%-100%, except for Vinclozolin.
    Additional Material: 3 Ill.
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  • 38
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    Journal of High Resolution Chromatography 4 (1981), S. 183-183 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; End-sealing technique for static coating ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 39
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    Journal of High Resolution Chromatography 4 (1981), S. 197-200 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 40
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    Journal of High Resolution Chromatography 4 (1981), S. 215-217 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Direct method for flavor evaluation ; Aromatic herbs analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A simple, quick method is described for GC head space evaluation of herbs and drugs. The proposed procedure consists of milling a weighed sample of a certain herb in a glass device like a Braun MXK coffee grinder, modified in such a way that a special gas-tight valve allows the direct sampling of the vapors. Glass capillary columns are used to analyze the head space volatiles. Some preliminary applications of the method are given.
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  • 41
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    Journal of High Resolution Chromatography 4 (1981), S. 232-232 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Static coating ; Sealing method ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 42
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    Journal of High Resolution Chromatography 4 (1981), S. 237-239 
    ISSN: 0935-6304
    Keywords: HPLC fluorescence detection ; Pre-column derivatization ; Glibenclamide ; Serum drug levels ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 43
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    Journal of High Resolution Chromatography 4 (1981), S. 253-254 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 44
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    Journal of High Resolution Chromatography 4 (1981), S. 42-42 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 45
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    Journal of High Resolution Chromatography 4 (1981), S. 81-83 
    ISSN: 0935-6304
    Keywords: HPLC bonded phases ; Derivatisation of aminopropyl silica ; Resolution of enantiomers ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
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  • 46
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    Journal of High Resolution Chromatography 4 (1981), S. 308-308 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 47
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    Journal of High Resolution Chromatography 4 (1981), S. 38-39 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Fused silica capillary column ; Activity ; Hydroxylated surfaces ; Dehydroxylated surfaces ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 48
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    Journal of High Resolution Chromatography 4 (1981) 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 49
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    Journal of High Resolution Chromatography 4 (1981), S. 83-85 
    ISSN: 0935-6304
    Keywords: HPLC ; Nanoliter volume detector ; 1.5 × 10-5 AU noise (0.1 μl 0.1% benzene in methanol) ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
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    Journal of High Resolution Chromatography 4 (1981), S. 95-96 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 51
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    Journal of High Resolution Chromatography 4 (1981), S. 132-134 
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Ion-pair reversed-phase HPLC ; Chlorhexidine in pharmaceutical preparations ; Quantitation within 0.66-3.41% relative standard deviation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 8 Ill.
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  • 52
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    Journal of High Resolution Chromatography 4 (1981), S. 142-142 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 53
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    Journal of High Resolution Chromatography 4 (1981) 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 54
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    Journal of High Resolution Chromatography 4 (1981), S. 247-248 
    ISSN: 0935-6304
    Keywords: Gas Chromatography ; No simple relationship found between polarity of polysiloxane phases and N-TFA amino acid butyl esters ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In this work, we have investigated the influence of the polarities and structures of 13 polysiloxanes on the retention of some N-TFA N-butyl esters of amino acids. Gehrke [1] was the first to examine the possible relationship between polarity and retention data of amino acid derivatives for 9 stationary liquid phases. He concluded that the molecular structure of the stationary liquid phase is an important factor determining retention.
    Additional Material: 3 Ill.
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  • 55
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    Journal of High Resolution Chromatography 4 (1981), S. 287-291 
    ISSN: 0935-6304
    Keywords: Thin layer chromatography, TLC ; Ion exchange resin plates/tert-butyl alcohol, H2O, Na2HPO4, Nacl; ethanol, H2O, Na-acetate, NaCl ; Sulfonamides and other chemotherapeutic substances ; Qualitative analysis ; Effect of pH variation on Rf values ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A thin-layer chromatographic method is described for sulfonamides and some other chemotherapeutic compounds. Ion-exchange resin precoated plates (Fixion 50 × 8 Na+, Chinoin, Budapest or Polygram Ionex-25 SA-Na, Macherey-Nagel and Co.,Düren, FRG) are employed with Na2HPO4 or Na-acetate (adjusted to different pH values) containing NaCl and tert-butyl alcohol or ethanol as developing solvent system. For qualitative and/or semiquantitave determinations the chromatograms can be stained: (A) with o-tolidine, after chlorination; (B) with p-dimethylaminobenzaldehyde; (C) with p-dimethylaminocinnamaldehyde.
    Additional Material: 3 Ill.
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  • 56
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 346-347 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; WCOT-glass capillary ; Organophosphorus insecticides ; Pesticides ; Metabolites ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This article considers the separation of 23 organophosphorus insecticides to which two metabolites have been added. The high resolution of the column permits detection of the Z and E isomers of three of these insecticides.
    Additional Material: 1 Ill.
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  • 57
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 361-362 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 58
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 366-384 
    ISSN: 0935-6304
    Keywords: GC/MS ; Capillary columns, fused silica ; Priority pollutants ; Quantitation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Operational characteristics have been determined for fused silica capillary column (FSCC) GC/MS as applied to “extractable” priority polutants. Chromatographic data show excellent relative retention time (RRT) intralaboratory precision and interlaboratory accuracy when multiple internal standards are empolyed. Potential chromatographic problems, such as column overload and “double peaking”, are addressed. Response factor relative standard deviations (RSD) at 50 ng for most of the extractable priority pollutants over the long term indicated precise determination (i.e. RSD generally ≤ 10%). Linearity was demonstrated over two orders of magnitude for FSCC GC/MS analysis of compounds with relatively low and high RF (response factor) values. Potential quantitative problems, such as saturation, are discussed. For certain aromatic priority pollutants interlaboratory RF agreement was observed. This was noted as perhaps the most important property of FSCC GC/MS analysis when the multiple internal standard approach is utilized. Determinations of extractable priority pollutants are directly compared for paced column GC/MS and FSCC GC/MS analysis of separate and composited extracts. For six extracts analyzed in triplicate, the latter configuration was shown to produce more consistent results. In view of the superior analysis logistics of composite extract FSCC GC/MS analysis, this approach was established as the preferred method for the analysis of priority pollutants classified as extractable.
    Additional Material: 12 Ill.
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  • 59
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 393-397 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, fused silica ; Simultaneous detection ; Splitting capillary effluent ; Detection limit of 1 ppm Archlor 1254 ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A state-of-the-art gas chromatographic system for automatic simultaneous detection of halogenated and normal hydrocarbons has been developed, which consists of a standard Hewlett-Packard 5880 with a fused silica capillary column, whose effluent is split between the standard flame ionization detector and a Tracor Hall electroconductivity detector. The system provedes excellent capillary chromatography results and high sensitivity for halogenated compounds (a detection limit of 1 ppm Archlor 1254 in fuel oil). Reliability has been provides in the daily analysis of complex environmental samples.Emergency response cleanup and the containment of hazardous chemical spills and chemical dump sites forces one to deal with samples that are very complex. The contain large numbers of naturally occurring organic compounds and varying types of organic pollutants. Capillary chromatographic techniques of gas chromatographic and gas chromatographic/mass spectrometric analyses are necessary to achieve the resolution required for the analysis of these samples.
    Additional Material: 10 Ill.
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  • 60
    ISSN: 0935-6304
    Keywords: Thin layer chromatography ; Use of molten solids as mobile phase advantageous ; Migration at higher temperature (80-180°C) studied ; Correction of front position for unsaturated flow possible ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper considers some aspects of a new TLC technique using a molten mobile phase which is solid under ambient conditions. The flow of high-boiling mobile phase at elevated temperature in thin-layer chromatography has been investigated and it is shown that the equation Zf2 = kt is not applicable to migration of the high boiling mobile phase front. The flow stability of the high-boiling mobile phase is noted. It is suggested on the basis of studies of concentration profiles of the solidified mobile phases by scanning photometers that the shape of the mobile phase concentration profile be taken into account in calculation of Rf Values.
    Additional Material: 7 Ill.
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  • 61
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981) 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 62
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    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 500-507 
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; Weak anion exchanger ; Octadecyl bonded packing ; Urine analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The analysis of ultraviolet-absorbing constituents in urine was undertaken by anion-exchange and reversed-phase liquid chromatography. A newly developed anion exchanger (TSK-IEX540 DEAE) was used for ion-exchange mode liquid chromatography and its high polarity and physical strength greatly reduced the analysis time. Up to 80 peaks were resolved within 30 min. Hydrophobicity values were used to predict the retention times of acids in reversed-phase mode liquid chromatography.
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  • 63
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    Journal of High Resolution Chromatography 4 (1981), S. 523-524 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Split injection ; Injection time influence ; PAH ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 64
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    Journal of High Resolution Chromatography 4 (1981), S. 533-534 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; End-sealing method for static coating ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 65
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    Journal of High Resolution Chromatography 4 (1981), S. 532-533 
    ISSN: 0935-6304
    Keywords: Micro high performance liquid chromatography, MHPLC ; Fourier transform infrared spectrometry, FT- IR ; Combination of MHPLC and FT-IR ; KBr buffer memory technique ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
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  • 66
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    Journal of High Resolution Chromatography 4 (1981), S. 546-546 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 67
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    Journal of High Resolution Chromatography 4 (1981), S. 557-560 
    ISSN: 0935-6304
    Keywords: Thin layer chromatography, TLC, HPTLC ; Reversed phase, chemically bonded ; Phenols ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 68
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    Journal of High Resolution Chromatography 4 (1981), S. 580-581 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Coal gasification tar ; Boiling point distribution ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
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  • 69
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    Journal of High Resolution Chromatography 4 (1981), S. 584-585 
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Reverse-phase mode ; TBAOAc/NH4OAc ion-pairing reagent circumvents problems ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 70
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    Journal of High Resolution Chromatography 4 (1981), S. 591-594 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 71
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    Journal of High Resolution Chromatography 4 (1981) 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 72
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    Journal of High Resolution Chromatography 4 (1981), S. 647-648 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Direct injection of silicone fluids method of choice for determining trace organic contaminants ; ≥ 1 ppm toluene determined ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 73
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    Journal of High Resolution Chromatography 4 (1981), S. 653-654 
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Diastereoisomer separation of organic sulfites ; Bis(2-aryl-3-oxotetrahydro-2H-1,2-oxazin-5-yl) sulfite ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 74
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    Journal of High Resolution Chromatography 4 (1981), S. 659-660 
    ISSN: 0935-6304
    Keywords: Thin layer chromatography, TLC ; Quantitation by TLC scanner ; Detection mode, reflection ; Measuring mode, absorbance ; RSD 3% for carbofuran and 1% for quinalphos ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 75
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    Journal of High Resolution Chromatography 4 (1981), S. 3-5 
    ISSN: 0935-6304
    Keywords: Molecular weight distribution ; Polyglycol GC stationary phases ; Carbowax ; Ucon ; Pluronic ; Emulphor ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The width of the molecular weight distribution (MWD) of polymer stationary phases influences their polarity and other gas chromatographic characteristics. The MWD of the most frequently used polyglycol-type stationary phases such as Carbowax, Ucon, Emulphor and Pluronic has been determined and compared. The MWD determined by gel chromatography allows for the characterization of the actual batch of a stationary phase, provides a check on its consistency and helps to screen out the apparently less promising phases.
    Additional Material: 4 Ill.
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  • 76
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    Journal of High Resolution Chromatography 4 (1981), S. 24-26 
    ISSN: 0935-6304
    Keywords: HPTLC ; HPLC ; GC/MS ; Methyl hydroxybenzoate (MHB) ; Propyl hydroxybenzoate (PHB) ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Different chromatographic methods for determination of methyl hydroxybenzoate and propyl hydroxybenzoate in pharmaceutical formulations are compared. Procedures for HPTLC, HPLC and GC/MS are given.
    Additional Material: 5 Ill.
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  • 77
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    Journal of High Resolution Chromatography 4 (1981), S. 37-38 
    ISSN: 0935-6304
    Keywords: HPLC ; Cardenolides in Convallaria majalis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Cardenolides of Convallaria majalis were separated by reverse-phase HPLC on Partisil PXS 10/25 ODS columns using 25% acetonitrile as mobile phase. Periplorhamnoside, convallatoxin and either convallatoxol or convalloside were resolved from glycoside mixtures. Convallatoxin and periplorhamnoside were identified in plant extracts, convalloside and convallatoxol were not resolved from non-cardenolide material present in leaf extracts.
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  • 78
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    Journal of High Resolution Chromatography 4 (1981), S. 79-80 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Glass capillary columns ; Barium carbonate ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
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  • 79
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    Journal of High Resolution Chromatography 4 (1981), S. 86-89 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Packed columns ; Multidimensional switching, MDSS ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A multidimensional switching system with two packed columns has been used for the analysis of volatiles. Use of a high-boiling solvent and the backflush mode permitted total resolution of the mixture discussed in a short analysis time.
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  • 80
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    Journal of High Resolution Chromatography 4 (1981) 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 81
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    Journal of High Resolution Chromatography 4 (1981), S. 102-108 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Series-coupled columns with different stationary phases ; Independent temperature control of columns ; Constant mass flow rate ; Retention time expressions ; Theory ; Selectivity control ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The technique of controlling chromatographic selectivity by the adjustment of column temperatures in systems of series-coupled columns is investigated by means of a general model incorporating the effects of temperature and mobile phase compressibility. In a previous article the performance of series-coupled columns was investigated under conditions of constant overall pressure drop. The present paper, on the other hand, considers systems in which a constant mass flow rate is maintained at all temperatures. The expressions derived for the effective partition coefficient and the retention time are compared with those of the previous paper. The numerical results for two-column systems exhibit the same major trends as those for constant pressure drop. It is shown that the effective partition coefficient is slightly dependent on the mass flow rate.
    Additional Material: 12 Ill.
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  • 82
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    Journal of High Resolution Chromatography 4 (1981), S. 127-129 
    ISSN: 0935-6304
    Keywords: Gas liquid chromatography ; Open tubular capillary columns ; Adsorption and resolution ; Hydrocarbons ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
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  • 83
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    Journal of High Resolution Chromatography 4 (1981), S. 130-131 
    ISSN: 0935-6304
    Keywords: Liquid chromatography/mass spectrometry ; Atrazine ; Quantification ; Triazine herbicides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 84
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    Journal of High Resolution Chromatography 4 (1981), S. 143-145 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 85
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    Journal of High Resolution Chromatography 4 (1981), S. 147-148 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 86
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    Journal of High Resolution Chromatography 4 (1981), S. 164-168 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Simultaneous detection with effluent splitter ; Element-specific detection ; Petroleum residues ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Two glass capillary gas chromatographic systems were equipped with inert effluent splitters which allowed simultaneous data acquisition using nonspecific and element-specific detectors. Simultaneous detection was achieved using the nonspecific flame ionization detector (FID) and the Hall® electrolytic conductivity detector (HECD) operated in either the sulfur-or the nitrogen-specific mode. Typical application of the simultaneous detection system as applied to analysis of petroleum residues is briefly described. The Hall electrolytic conductivity detector can be made element specific for halogen-, sulfur-, or nitrogen-containing compounds. Simultaneous detection enhances the information yield from a single sample injection and proves to be a powerful complementary technique when used with computerized gas chromatography/mass spectrometry.
    Additional Material: 5 Ill.
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  • 87
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    Journal of High Resolution Chromatography 4 (1981), S. 186-187 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Effluent splitter, preparation of ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
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  • 88
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    Journal of High Resolution Chromatography 4 (1981), S. 191-192 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 89
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    Journal of High Resolution Chromatography 4 (1981), S. 218-223 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Column preparation ; “Split” vs. “on-column” injection ; Amino acid analysis, quantitative 10 ng level ; Average relative standard deviation 1% ; Fish muscle protein analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A carefully Standardized technique is described for the preparation of glass capillary columns which can be used successfully for routine quantitative amino acid analysis. Comparison is made between two different modes of sample injection. Preliminary quantitative results from “split” injection and “on-column” injection techniques are evaluated statistically and it is concluded that the “on-column” system is a prerequisite for quantitative amino acid analysis by glass capillary gas chromatography. An analysis of fish muscle protein hydrolyzate illustrates an application of this technique and results are compared with those from a packed column analysis.
    Additional Material: 4 Ill.
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  • 90
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    Journal of High Resolution Chromatography 4 (1981), S. 224-227 
    ISSN: 0935-6304
    Keywords: Thin-layer adsorption chromatography ; Optimum separation ; Binary mobile phases ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The measure of the distribution of a mixture of substances in TLC with a binary mobile phase is expressed as ΔRM1,2i,j which represents the difference between the RM values of substances i and j on use of the binary solvent 1 + 2 as the mobile phase. The possibility of determining its maximum value at an optimum composition of the binary mobile phase is demonstrated in this paper. This value can calculated from experimental and theoretical functions RM1,2 = f(Φ1) of substance i and j. More simply, ΔRM1,2i,j can be calculated theoretically from easily measurable adsorption parameters of excess adsorption isotherms and from chromatographic parameters obtained for pure solvents. The ΔRM1,2i,j value calculated theoretically can be utilized in a pilot technique for determination of the optimum composition of the mobile phase in gradient liquid chromatography.
    Additional Material: 2 Ill.
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  • 91
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    Journal of High Resolution Chromatography 4 (1981), S. 437-443 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Gas chromatographic profiles ; n-Butylboronates ; Alkyl-oximes ; Primary prostaglandins ; 6-Keto prostaglandin F1α ; Thromboxane B2 ; Isomers of prostaglandin F1α ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This work describes the use of glass capillary columns (GCC) in the rapid concurrent analysis of primary prostaglandins (PGs) (e. g. PGE2, PGE2, PGF2α) and other functionally significant metabolites of arachidonic acid (AA) such as TXB2 and 6-keto PGF1α. The use of a new multistep mixed derivatization approach that generates the methyl esters of n-butylboronate, pentafluorobenzyloxime, trimethylsilyl ether derivatives of these compounds remarkably simplifies the GC profiling of the three main pathways of AA metabolism (PGs, thromboxane, and prostacyclin). Furthermore, isomeric species giving very similar or identical mass spectral patterns can be easily identified by their relative retention times on high efficiency capillary columns.
    Additional Material: 6 Ill.
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  • 92
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    Journal of High Resolution Chromatography 4 (1981), S. 276-279 
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; Linear elution strength mobile phase technique ; Calculation of eluent compostion ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The use of gradient elution in RP-HPLC is increasing. Aqueous buffers, such as phosphate at pH 1.7, with acetonitrile as the organic modifier are particularly advantageous as eluents with linear elution strength (LES). In the isocratic mode, under the experimental conditions used, fairly good linearity exists between logk' and xB (xBº is the volume ratio of the organic modifier, i.e. acetonitrile) with a correlation coefficient of 0.9995. The relationship between isocratic and gradient elution parameters, such as xB, xBº (starting composition in gradient elution), m (slope of the linear gradient), is transparent and the parameters of interest can be predicted fairly accurately using simple equations. The proposed system could be used in elaboration of a general retention index system for organic molecules in RP-HPLC.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 93
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 482-482 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 94
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 495-499 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, fused silica ; Mass spectrometry ; Interface, direct coupling ; On-column injection ; Aflatoxin B1 ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A novel fused silica capillary GC/MS direct coupling is described which ensures quantitative transfer of sample from the point of injection to the ion source of a mass spectrometer without contacting any surfaces other than the column wall. This device permits GC analysis of highly labile substances. Aflatoxin B1, previously considered unassayable by GC, can now be determined by GC/MS.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 95
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 309-314 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 96
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 292-292 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 97
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 292-292 
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 98
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 333-336 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Rapid analysis ; On-line analysis ; Low molecular weight hydrocarbons ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Rapid analysis is important for on-line chromatography. Gaseous or vaporized samples have been injected via heated gas sampling valves of less than 500 μl dead volume. The critical sampling and split problems could be solved by temperature programming. The general analysis described could be successfully used inter alia in scouting reactions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 99
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 357-358 
    ISSN: 0935-6304
    Keywords: Partition thin-layer chromatography ; Pesticides ; Separation of the s-triazine herbicides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 100
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 354-356 
    ISSN: 0935-6304
    Keywords: HPLC ; Derivatization of amino acids ; Effect of reaction pH on isoindole formation ; Effect of reaction time on isoindole formation ; 2-buffer program for HPLC separation of isoindoles ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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