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  • Electronic Resource  (1,384)
  • 1975-1979  (1,227)
  • 1965-1969  (157)
  • Engineering General  (978)
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  • Electronic Resource  (1,384)
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  • 1
    ISSN: 1435-1463
    Keywords: Cat ; electrical stimulation ; serotonin ; splanchnic nerves ; substance P
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The blood levels of serotonin (5-HT) and substance P (SP) in the portal vein were studied after splanchnic nerve stimulation in the cat. The portal levels of both substances were studied before, during and after splanchnic nerve stimulation. There was a twofold increase in 5-HT during stimulation whilst the SP concentration remained unchanged. These results suggest that the nervous control of the amine release into the portal stream and the mechanism that regulates the release of the polypeptide is not the same.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 27 (1979), S. 177-185 
    ISSN: 1432-0827
    Keywords: Calcification ; Bone ; Glycoprotein ; Golgi ; Osteoblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The elaboration of bone matrix glycoprotein by osteoblasts of alveolar bone was investigated by radioautography after the intravenous injection of3H-fucose into young rats. At selected times after injection, animals were sacrificed by intracardiac perfusion and demineralized specimens were prepared for light and electron microscope radioautography. At 5 and 10 min after injection, when the blood fucose level was high, silver grains were restricted to the spheroidal and cylindrical saccules of the Golgi apparatus. At 20 min membrane-limited secretory granules were also labeled. By 35 min, the blood fucose level had dropped and silver grains were detected over the apical cortical cytoplasm, in association with secretory granules located therein. Some grains were present over osteoblast processes and the adjacent prebone matrix. By 4 h most of the silver grains had left the cell. At that time they were observed over prebone, adjacent to osteoblast processes, as well as over the prebone-bone junction where a distinct band of label was noted. In demineralized preparations an electron-dense granular material was present at the prebone-bone junction in association with collagen fibrils. These findings provide evidence that osteoblasts in alveolar bone synthesize fucose-containing glycoprotein and indicate that the addition of3H-fucose occurs in the Golgi apparatus. The glycoprotein passes to the apical cortical cytoplasm by way of membrane-limited secretory granules, is exteriorized, and accumulates at the site where prebone transforms into bone (the prebone-bone junction). Since this is also the site of the calcification front, the deposition of labeled glycoprotein may be related to the deposition of bone mineral.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 27 (1979), S. 95-99 
    ISSN: 1432-0827
    Keywords: Bone ; Fluoride ; Analysis ; Food ; Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Recently published bone fluoride values from Iowa are very high compared to earlier reports, suggesting an increase in fluoride intake. Reanalysis of the Iowa specimens shows levels one-fourth those reported by the Iowa laboratory indicating an error in the original report. Seventeen bone specimens, collected from long-term residents of Rochester, New York, drinking 1 ppm F− water, had a mean value of 2085±270 ppm F− on an ashed-weight basis. This value is not significantly different from that predicted by the data of Zipkin et al. in 1958. These data, therefore, do not support the contention that there has been an increase in fluoride intake.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 28 (1979), S. 103-105 
    ISSN: 1432-0827
    Keywords: Bone ; Photodensitometry, X-ray ; Radiography ; Spectrophotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A conventional spectrophotometer was used to do radiographic photodensitometry of bone mass. An experiment to produce osteoporosis in guinea pigs required quantification of changes in bone mass. Lack of a densitometer necessitated finding another method for quantification. The method developed involved using industrial film in a closed X-ray system. Optical density of X-rays was then read in a spectrophotometer at nine femur locations. Highly significant correlations between optical density and femur calcium content and femur dry weight per millimeter were obtained. Histological sections from an animal with low optical density measurements confirmed bone loss by showing decreased cortical width and loss of trabecular bone. In addition to using readily available equipment, this method offers the advantage of being able to detect bone loss in specific areas of the bones. In addition, bones are left intact and can be used for other purposes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0827
    Keywords: ESR ; Bone ; Enamel ; CO3-Apatite ; Paramagnetism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Carbonate-containing hydroxyapatite, enamel, and bone were irradiated by an X-ray and investigated between 77° and 350°K by means of electron spin resonance (ESR) spectroscopy. The ESR spectrum of enamel irradiated at 77°K in vacuum and observed at the same temperature was almost the same as that of the carbonate-containing hydroxyapatite. The temperature dependence of signal intensities confirms a spin-energy exchange between the mineral and organic constituents in bone, but in enamel no or very little spinenergy exchange between the mineral and organic constituents. Considerable similarity among the ESR spectra of enamel, bone, and carbonate-containing apatite was obtained after X-ray irradiation in air at 300°K with both an X-band and a Q-band ESR spectrometer. The Q-band spectrum can be interpreted in terms of two paramagnetic species. One is identified as a CO 3 3− anion radical which has an axial symmetry withg factors of 2.0029 and 1.9972. The other species is found to be centered atg=2.0019.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 28 (1979), S. 33-36 
    ISSN: 1432-0827
    Keywords: Infrared, visible light ; Bone ; Collagen ; Apatite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Photovoltaic effect (infrared and visible light) is observed in bone and its two major components, collagen and apatite, at room temperature. A dimunition in the magnitude of photovoltage is observed after exposure to ultraviolet light in all the cases. The drift mobility of the charge carriers is obtained by measuring I versus V relationships in sandwich samples and relating them to the permitivity of the medium. Lifetime of the injected carriers is measured in the usual way. The data are consistent with the hypothesis that the effects are due to protonic conduction phenomena.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 34 (1979), S. 273-286 
    ISSN: 1432-1106
    Keywords: Stimulus velocity ; Single cell responses ; LGN ; Area 17 ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Neuronal responses to moving visual stimuli were recorded in the lateral geniculate nucleus (LGN) and area 17 of cats. Response duration (DE), number of spikes (NT), and mean frequency (FM) were estimated from the response histograms and analysed for their dependence on stimulus velocity. In the LGN, for about 2/3 of cells these response parameters changed monotonically with velocity up to about 100 °/s. In 1/3 of the cells, the response frequency was tuned to velocity. The speed at which individual cells reached a peak or plateau firing rate was correlated with their receptive field size. In area 17, most neurones were tuned to velocity. Nine out of 59 cells were insensitive to stimulus speed in that they responded equally well at stimulus velocities up to about 10 °/s. The results suggest that at higher levels in the nervous system information about velocity is represented in discrete groups of neurones. It is pointed out that different response parameters may be relevant for different perceptual phenomena associated with movement. The significance of integrational properties and lateral inhibition of nerve cells for the development of complex response properties is discussed.
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  • 8
    ISSN: 1432-1106
    Keywords: Thermoreceptors ; Gastro-intestinal tract ; Vagal nerve ; C Fibres ; Cat ; Thermorecepteurs ; Tractus gastro ; Intestinal ; Nerf vague ; Fibres amyéliniques ; Chat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Chez des chats anesthésiés, des décharges sensitives vagales ont été enregistrées dans le ganglion plexiforme à l'aide d'électrodes extracellulaires en verre. Nous avons trouvé au niveau de l'antre et du duodénum, des récepteurs toniques, activés par des solutions chaudes (38–51 ° C avec un optimum à 46–49 ° C) ou froides (36–10 ° C avec un optimum à 12–10 ° C). Ces récepteurs ne répondent ni aux stimulus mécaniques (compression et distension des viscères), ni aux stimulus chimiques (perfusion avec des solutions glucosées ou acides). Ces récepteurs n'appartiennent donc pas au groupe des récepteurs polymodaux, mais ils doivent être considérés comme de véritables thermorécepteurs spécifiquement sensibles au chaud ou au froid. Les thermorécepteurs de la région gastro-duodénale sont connectés à des fibres vagales amyéliniques (vitesse de conduction: 0,8–1,4 m/s). Par ailleurs, le rôle éventuel des thermorécepteurs vagaux de la région gastro-duodénale dans la régulation de la motilité digestive a été recherché. En dérivant simultanément l'EMG en plusieurs points du tractus gastro-duodénal, il a été possible de démontrer que les stimulations froides et chaudes du duodénum, qui mettent en jeu les thermorécepteurs, provoquent une inhibition de l'activité électrique de l'antre. Les modifications persistent apèrs bisplanchnectomie, mais disparaissent complètement après bivagotomie. A partir de ces faits, nous en avons conclu que les thermorécepteurs vagaux sont impliqués dans la régulation nerveuse de la motilité gastro-duodénale.
    Notes: Summary In anaesthetized cats, sensory vagal units were recorded in the nodose ganglion by means of extracellular glass microelectrodes. In the antrum and the duodenum we have found receptors tonically activated by warm (38–51 ° C with an optimum at 46–49 ° C) or cold (36–10 ° C with an optimum at 12–10 ° C) solutions. These receptors did not respond to mechanical stimuli (compression and distension of the viscera) and to chemical ones (perfusion with glucose and acid solutions). Thus they did not belong to polymodal type, but they must be considered as true thermoreceptors, specifically sensitive to warm or cold stimulations. The gastro-duodenal thermoreceptors were connected to non-medullated vagal fibres (conduction velocity: 0.8–1.4 m/s). On the other hand, the role of the gastro-duodenal vagal thermoreceptors in the regulation of the digestive motility was studied. By using several electromyographic recordings, it was possible to show that the cold and warm stimulations of the duodenum which elicited thermoreceptor responses, induced an inhibition of the electrical activity of the antrum. The changes persisted after bisplanchnectomy, but disappeared completely after bivagotomy. From these facts it was concluded that the vagal thermoreceptors were involved in the nervous regulations of the gastro-duodenal motility.
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  • 9
    ISSN: 1432-1106
    Keywords: Cooling ; Ventrolateral thalamic nucleus ; Pyramidal system ; Reaction time ; Ballistic movement ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five cats were trained to perform a forelimb ballistic flexion on a reaction time paradigm including an upper limit of about 400 ms for reinforcement (food pellets). They were implanted with a cyrogenic probe thermically insulated, except at the tip, by a vacuum jacket (outer diameter, 1.1 mm). Four cats had the probe inserted into the ventrolateral thalamic nucleus (VL), contralateral to the moving limb. During cooling they showed increased reaction times, which remained constant throughout daily sessions performed during many weeks, independent of the foreperiod but varying from 25 to 100 ms according to the subject. The temperatures used to upset the reaction times varied from +10 ° C to −8 ° C, depending on the localisation of the probe and on the insulation of the silver tip used to prevent nervous tissue reaction, but for each subject the reaction times always increased when the temperature was lowered. The fifth cat, with a probe inserted between VL and the Centre Median, showed a decrease of reaction times on cooling to 0 ° C and an increase of the reaction times for a cooling at −10 ° C. For one of the four cats with a probe properly inserted into the VL, strain-gauges were stuck on the lever to measure the latency of the decrease of the pressure exerted by the subject when the subject initiated the forelimb flexion in response to the CS. Reaction times and latencies of pressure changes were closely correlated with the movement onset, and they were equally delayed during cooling. This result demonstrates that it is not by slowing down movement velocity that reaction times are upset during VL cooling but by delaying the movement onset.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1106
    Keywords: Retractor bulbi motoneurones ; Accessory abducens nucleus ; 6th nerve ; Oculomotricity ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Motoneurones innervating the retractor bulbi muscle in the cat have been identified by retrograde labelling with horseradish peroxidase, by intracellular recording and by intracellular staining with horseradish peroxidase. Their somata are found in an accessory abducens nucleus, analogous to that described in some other species, which consists of a narrow column of cells situated in the lateral tegmental reticular field, above the superior olive and medial to the facial nerve. This column of cells extends over approximately 1.5 mm from P 5.5 to P 7. The retractor bulbi motoneurones number from 80 to 120 and have large, elongated somata which give rise to five or six major dendrites. Their axons cross the reticular formation in a dorso-medial direction to pass through the principal abducens nucleus before turning to leave the brain stem in the 6th nerve. Antidromic latencies ranged from 0.4 to 0.7 ms. Some retractor bulbi motoneurones could also be activated antidromically by stimulation of the lateral rectus muscle nerve.
    Type of Medium: Electronic Resource
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 25-36 
    ISSN: 1432-1106
    Keywords: Retinal ganglion cells ; Cat ; Contrast reversal stimulus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A contrast reversal (alternating phase) stimulus was used to study the responses of 150 retinal ganglion cells from 15 adult cats. Because the majority of the cells did not show perfect linear spatial summation, a ratio of the firing rates at two time periods was used to express the degree of nonlinearity. Y-cells showed a high degree of nonlinearity, and their mean null ratio was significantly lower than that of X-cells. With the stimulus at the null position, X-cells had an unmodulated discharge rate which was significantly higher than maintained activity, while the firing rate of Y-cells was lower than maintained activity. With the stimulus placed at an eccentric position in the receptive field, X-cells responded in a sustained manner, while Y-cells respond transiently. Because of these observations, we conclude that X-cells correspond to the sustained cells, while Y-cells correspond to the transient cells.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 161-175 
    ISSN: 1432-1106
    Keywords: Cerebral cortex ; Superior colliculus ; Cat ; Horseradish peroxidase method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The retrograde labeling of cortical neurons with horseradish peroxidase (HRP) was used to investigate the morphological features of neurons in various cortical areas projecting to the superior colliculus in the cat. Corticotectal cells were found to be labeled in layer V of the entire cerebral cortex. The number of labeled cells and their locations varied according to the sites of injections of HRP in the colliculus. Most of the Corticotectal cells identified in the present study were small (9–20 μm in diameter, 66%) and medium (20–40 urn, 30%) pyramidal neurons and only 4% of them were large (more than 40 μm). The labeled cells, 261 in total number, had somal diameters of 20.8±8.0 μm (mean and SD). The range of sizes of the labeled neurons was different in different cortical areas. For example, the labeled neurons in the Clare-Bishop area had a greater proportion of large diameter cells than in other areas. The present findings are largely in agreement with the previous data of anterograde degeneration methods with respect to the topographical correlation of the Corticotectal projections. However, in some cortical areas, e.g., the sensorimotor and the first visual (area 17) cortex of the lateral surface of the hemisphere, relatively small numbers of Corticotectal neurons appear to have been labeled by retrogradely transported HRP. The sparsity of the labeled neurons in certain cortical areas may reflect the existence of Corticotectal neurons with axon collaterals supplying brain structures other than the superior colliculus.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 269-285 
    ISSN: 1432-1106
    Keywords: Cerebral cortex ; Pontine nuclei ; Cat ; Horseradish peroxidase method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Horseradish peroxidase (HRP) injections in various portions of the cat pontine nuclei resulted in retrograde labeling of neurons in layer V of the ipsilateral cerebral cortex. Corticopontine neurons, pyramidal in type, have been found to be labeled in the entire cortex, confirming the previous findings of anterograde degeneration studies. Most (91%) of the labeled cells were 14–26 μm in diameter (mean 19.4±4.5 μm SD). Small (10–20 μm) and medium (20–40 μm) cells represent 51.5% and 47.7%, respectively, of the total number of the labeled neurons. The populations of the neurons of various sizes were almost identical in different cortical areas, and were different from the populations of corticoreticular and corticospinal cells. Corticopontine cells were well labeled in experimental cases of 3-days' survival time, confirming the topographical organization established previously by degeneration studies for this projection system. However, in cases of shorter survival time (20–27 h), the number of labeled neurons was very small. The relative paucity of labeled Corticopontine neurons in the sigmoid and lateral gyri is discussed with reference to other cortical descending neurons (e.g., the corticotectal, corticoreticular and corticospinal) which have hitherto been identified morphologically as well as physiologically.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 395-406 
    ISSN: 1432-1106
    Keywords: Cat ; Spinal cord ; Phrenic motoneurons ; Neuron geometry ; Horseradish peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina IX of the lower cervical cord. Cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments C5 and C6. The phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for the crossing of phrenic axons in the cord. Assessment of cellular geometry and intercellular relationships demonstrated that phrenic cell diameters approximated a normal distribution with a single peak at 26 μm while longitudinal cell lengths averaged 76 μm. Cells of different size were mixed randomly at all levels of the nucleus. The minimum distance between cells was about 10 μm and the maximum cell packing density approached 2 cells per 106 μm3. The results confirm the location of the cat phrenic nucleus, extend the knowledge of phrenic motoneuronal geometry, and provide an anatomical basis for the understanding of recruitment and synchronization phenomena within the phrenic nucleus.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 465-477 
    ISSN: 1432-1106
    Keywords: Cat ; Monkey ; Lateral geniculate nucleus ; Visual nondominant suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have studied the suppression of firing in single LGN cells of cat and monkey in response to visual stimulation of the nondominant eye. In the cat LGN most of the cells of each of the main laminae show this nondominat suppression. X cells having their dominant input from the ipsilateral eye were suppressed to a significantly greater degree than any other cell type in the cat LGN. In the monkey LGN nondominant suppression was absent in all 19 X-like cells studied, whereas 6 of 21 Y-like cells showed nondominant suppression. Thus nondominant suppression is present in the magnocellular laminae of the monkey LGN, where the Y-like cells are found, but appears to be absent from the parvocellular laminae, where the X-like cells are found.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 479-494 
    ISSN: 1432-1106
    Keywords: Thermosensitivity ; Lesions ; Spinal cord ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The behavioural thermosensitivity of cat paws was examined before and/or after restricted uni- and/or bilateral lesions had been made in the spinal cord between the first and fifth cervical segments. Unilateral lesions of the lateral funiculus, which involved at least its whole width at the level of the central canal, reproducibly were found to interfere with the contralateral sensitivity for temperature increases and/or decreases. No corresponding thermosensory deficiencies were found after unilateral lesions involving the ventral spinal quadrant or the dorsal funiculus. Various bilateral and combined lesions were made, but no cat ever developed thermoanaesthesia. The bilateral lesions included bilateral transections of: the middle parts of the lateral funiculi, the dorsal halves of the lateral funiculi, the dorsal funiculi, and the ventral spinal half. Most of our knowledge about peripheral behavioural thermosensitivity after spinal cord injury is based on observations of human patients, especially after anterolateral chordotomies. The present finding of contralateral thermosensory deficiencies after lesions of the middle part of the lateral funiculus fits with some of the clinical reports. The present failure to cause thermoanaesthesia, on the other hand, is inconsistent with the theory of a single ascending spinal pathway for behavioural thermo-sensitivity, which has emanated mainly from the clinical observations.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 35 (1979), S. 495-510 
    ISSN: 1432-1106
    Keywords: Pretectal nuclei ; Superior colliculus ; Visual response pattern ; Retinal input types ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Single unit recordings from 220 units were obtained from the nuclei praetectalis anterior (NPA) and posterior (NPP) of 30 immobilized, anesthetized cats. Quantitative analysis of pretectal (PT) visual activity was mainly based on recordings from the NPP. For comparison, 160 collicular (CS) neurons were studied. A strong sensitivity for moving objects was evident in both samples. The following main types of PT activity were categorized: (A) slow movement, direction-selective units (21%); (B) slow movement, nondirection-selective units (19%); (C) units nonselective for stimulus velocity and direction (24%); (D) jerk movement selective, nondirection-selective units (36%). Latency measurements following single shocks to optic chiasm (OX) and tract (OT) showed mainly slow conducting fiber input to the PT and CS which can be divided into two different groups by conduction properties and synaptic delay: direct W-input and delayed W-input. Fast Y-fiber input of both types, direct and indirect, was recorded at both sites, PT and CS.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 36 (1979), S. 191-194 
    ISSN: 1432-1106
    Keywords: Hypercomplex cells ; Area 18 ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Single unit recording has revealed the same orientation sensitive cell classes in cat area 18 as are to be found in area 17. These include particularly the various types of hypercomplex cell belonging to the S, C, and B cell families.
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 36 (1979), S. 285-300 
    ISSN: 1432-1106
    Keywords: VL nucleus ; Single units ; Reaction time ; Movement initiation ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Unrestrained cats performed ballistic forelimb flexion movements triggered by an auditory stimulus (CS) on a simple reaction time (RT) paradigm. During the variable foreperiod the subject was required to hold down a lever and to release it on presentation of the CS. The RTs ranged from 200 to 300 ms. The activity of single neurons of the ventrolateral nucleus of the thalamus (VL) was recorded bilaterally. More than 40% of the 166 units recorded in the VL contralateral to the performing limb presented, after the CS, changes of activity with a latency less than 100 ms and were classified into three types: (1) Twenty-five units had a short latency transient increase of activity 10 to 30 ms after the CS, followed by a longer increase or decrease in activity. Short latency increase as well as subsequent increase of the firing rate were not correlated to the RTs. (2) Twenty-nine units showed a 40–60 ms latency increase of activity which lasted long enough to continue during the forelimb movement. These units displayed a correlation between the RTs and the mean firing rate measured in the 40–100 ms period after the CS. The more the cells were activated, the shorter the RTs. (3) Fifteen units presented a reciprocal pattern of discharge with respect to the type (2) units. The firing rate decreased with latencies ranging from 20 to 90 ms after the CS. Only 14,5% of the 96 units recorded in the VL ipsilateral to the performing limb presented changes of activity starting in the 100 ms period following the CS. Background firing levels as well as phasic activity were rather low compared to those observed contralaterally. Sixteen units showed burst activity while the cat was performing but burst pattern was not time-related to the task. In an unconditioned animal, a very low level of activity and an absence of modulations were observed in both VLs.
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 37 (1979), S. 41-47 
    ISSN: 1432-1106
    Keywords: Eye alignment ; Cat ; Visual experience ; Maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the developing kitten, the alignment of the pupils changes from strongly divergent to almost parallel. The visual axes, however, seem to stay almost parallel throughout this period. The influence of early visual experience on this development is examined in the present study. The results suggest that the development of eye alignment is not controlled by visual experience, but depends on maturational processes, and that normal visual input serves only to halt these processes at the appropriate time.
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  • 21
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    Electronic Resource
    Springer
    Experimental brain research 37 (1979), S. 177-181 
    ISSN: 1432-1106
    Keywords: Cat ; Autoradiography ; Abducens nucleus ; Primary vestibular fibers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Primary vestibular projections to the ipsilateral abducens nucleus were found in cats by injecting anterograde tracer substances (radioactive proline and fucose) into Scarpa's ganglion. Labelling was observed in the rostral and middle portion of the abducens nucleus.
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  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 37 (1979), S. 405-416 
    ISSN: 1432-1106
    Keywords: Cerebellum ; Purkinje Cells ; Splanchnic mechanoreceptors ; Cat ; Cervelet ; Cellules Purkinje ; Mécanorécepteurs splanchniques ; Chat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les activités simple et complexe de cellules de Purkinje du cervelet du Chat (zone vermienne des lobules V et VI) ont été enregistrées au moyen de microélectrodes extracellulaires. Ces réponses étaient obtenues par stimulation de différents types de mécanorécepteurs du tractus gastro-intestinal: récepteurs musculaires connectés à des fibres C, récepteurs péritonéaux de mouvement connectés à des fibres Aγδ ou B, corpuscules de Pacini connectés à des fibres Aβ. Les caractéristiques des fibres splanchniques projetant sur cette aire cérébelleuse ont été définies. Des convergences splanchno-somatiques, splanchno-viscérales et splanchno-corticales ont été mises en évidence.
    Notes: Summary Climbing and mossy fibre activity in Purkinje cells of cat cerebellum (vermis part of lobules V and VI) were recorded by means of extracellular microelectrodes. Responses were obtained by stimulation of different types of mechanoreceptors in the gastro-intestinal tract: muscular receptors connected with C fibres, peritoneal movement receptors connected with Aγδ or B fibres, Pacinian corpuscules connected with Aβ fibres. The characteristics of the splanchnic fibre projections on this cerebellar area were defined. Splanchno-somatic, splanchno-visceral, and splanchno-cortical convergences were demonstrated.
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  • 23
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 37 (1979), S. 609-613 
    ISSN: 1432-1106
    Keywords: Orientation subunits ; Visual cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The orientation domain in the cortical visual areas of anesthetized cats has been investigated by employing the 14C-Deoxyglucose technique (Sokoloff et al., 1977). Orientation subunits (OS) are seen in the first (V1), the second (V2) and the third visual area (V3) as well as in the visual areas of the suprasylvian sulcus. In the latter regions OS are less elaborated than in V1, V2, and V3. The OS are continuous through all cortical layers; in V1 however, only weak label is detected in layer 4C. In V1, V2, and V3 the width of the OS is about 0.4 mm and the average distance between two OS centers is 0.9 mm. The spatial pattern of the OS seems to be more regular in the visual field periphery than in regions representing the vertical meridian.
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  • 24
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    Skeletal radiology 4 (1979), S. 72-78 
    ISSN: 1432-2161
    Keywords: Sarcomas ; Bone ; Neoplasms ; Radiation induced
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Historically, the literature reveals that the incidence of radiation induced bone sarcomas is very low. Details related to epidemiology cannot be identified, however, because of the difficulty of identifying precisely the patient population at risk for development of the radiation induced sarcoma. The change in character of practice in cancer management with ever increasing numbers of patients receiving both radiation therapy and chemotherapy should alert physicians to the potential for increased incidence of this rare and unusual tumor.
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  • 25
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    Anatomy and embryology 155 (1979), S. 323-331 
    ISSN: 1432-0568
    Keywords: Telencephalon ; Choroid plexus ; Lateral ventricle ; Blood vessels ; Micro Corrosion cast ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Corrosion casts of the complete vascular network of the choroid plexus of the lateral ventricle in the cat brain were studied in SEM using the injection-replication method. The villi of this plexus are located only on its supero-anterior and infero-posterior parts, being most densely packed in the former region, close to the interventricular foramen. The capillaries of the villi display small nodular thickenings, which suggest the presence of small, sinusoidal dilatations. The main vessel supplying the plexus with blood is the anterior choroidal artery. The plexus is also characterized by a particularly rich venous network, which is drained by a prominent (main) choroid vein. The vascular organization of the choroid plexus of the lateral ventricle in cat is compared to that of the corresponding plexuses in other mammals.
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  • 26
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    Acta neuropathologica 48 (1979), S. 55-58 
    ISSN: 1432-0533
    Keywords: Lafora-like bodies ; Morphogenesis ; Glycogen metabolism ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Lafora-like bodies in an 8-year-old cat were studied light and electron microscopically and histochemically. In addition to Lafora-like bodies composed of branching filaments, glycogen granules and electron-dense materials, abnormal accumulations of glycogen granules attracted attention. The most remarkable features were the developmental processes of the branching filaments originating directly from glycogen granules. Lafora-like bodies in the present study showed ultrastructural, histochemical, and enzymatic similarities to those described in the previous reports in Lafora's disease, glycogenosis and other cases. From these results, a certain disturbance of the glycogen metabolism is considered to be probably related to the productive mechanism of Lafora-like bodies.
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  • 27
    ISSN: 1432-0428
    Keywords: Insulin ; NSILA-S ; adipocytes ; hypophysectomized rats ; insulin-receptor ; NSILA-S-receptor ; glucose metabolism ; glucose transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Isolated fat cells from normal and hypophysectomized rats have been compared with respect to: 1) binding of insulin and NSILA-S, and 2) effects of these two hormones on glucose transport and metabolism. Although both insulin and NSILA levels were decreased in the serum of hypophysectomized rats, insulin binding was decreased to about 63% of normal, whereas NSILA-S binding remained unchanged. Basal lipogenesis was similar in adipocytes of normal and hypophysectomized rats, but was not stimulated by either insulin or NSILA-S. Similarly, neither of the two hormones stimulated the net gas exchange of “intact” fat pads from hypophysectomized rats. In striking contrast to these findings, 3-O-methylglucose transport in unstimulated fat cells of hypophysectomized rats proceeded at a maximal rate which was not further enhanced by insulin or NSILA-S. These results suggest that the lack of one or several hormones of the pituitary causes one or several enzyme deficiencies responsible for the limited rate of lipogenesis, which otherwiese would proceed at a very rapid rate because of unrestrained glucose transport.
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  • 28
    ISSN: 1432-0428
    Keywords: Insulin ; glucagon ; rat ; fetus ; neonate ; isolated islets ; glucose ; arginine ; leucine ; somatostatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Islets were isolated by mild collagenase digestion and microdissection from rat fetuses 2 days before term and pups 1 or 2 days after birth and their insulin and glucagon secretion studied in vitro. Fetal B cells were stimulated by 16.7 mmol/l glucose, 20 mmol/l leucine or 20 mmol/l arginine. Fetal A cells were not affected by glucose or leucine, but were significantly stimulated by arginine. Somatostatin abolished the effect of arginine on both IRI and IRG output. Neonatal islets proportionally released more insulin and glucagon than their fetal counterparts, but reacted to the tested agents in a similar fashion. During the perinatal period, pancreatic insulin storage increased at a higher rate than that of glucagon. It is concluded that fetal B cells are equipped with sensors to a variety of agents and able to modulate their secretory rate according to the concentration of these agents. A cells are reactive to arginine 2 days before term but do not become glucose reactive until several days after birth.
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  • 29
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    Diabetologia 16 (1979), S. 201-206 
    ISSN: 1432-0428
    Keywords: Insulin ; glucose ; newborn ; early insulin response to feeding ; insulin in suckled infant ; newborn lambs ; newborn rabbits ; atropine and insulin secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Developmental changes in the plasma insulin response to milk ingestion have been determined in neonatal lambs fed by bottle and in naturally suckled infant rabbits. In lambs at 4 days of age and older plasma insulin increased within 5 min of suckling and declined again before increasing to a second peak 60 to 120 min after the feed. The early insulin peak was not associated with any change in plasma glucose and was absent in lambs at 1 day of age. When the early insulin peak was absent the increase in plasma glucose following the meal was greater. The early, apparently reflex, release of insulin also occurred in some ‘sham-fed’ lambs. Atropine administration before feeding abolished the early peak in insulin. A marked early peak of insulin release, not accompanied by any consistent change in plasma glucose, was also observed in naturally-suckled infant rabbits at 1, 5, 10 and 15 days after birth.
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  • 30
    ISSN: 1432-0428
    Keywords: Insulin ; glucagon ; portal blood ; glucose ; arginine ; liver cirrhosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The portal vein was catheterized via the umbilical vein under local anaesthesia in 10 non-diabetic subjects about to undergo exploratory laparotomy and in 8 patients with liver cirrhosis. Immunoreactive insulin (IRI) and glucagon (IRG) were assayed in portal and peripheral blood before and during IV infusion of glucose (0.33 g/kg) or arginine (25 g). Basal peripheral plasma (IRI) levels were raised in cirrhotic patients (19±2 versus 10±1 μU/ml; P〈0.001). Basal portal insulin values, however, did not differ in the two groups. After glucose cirrhotic patients had higher peripheral insulin concentrations, compared to controls, significant at 45 and 60 minutes. In contrast portal insulin levels were higher in controls than in cirrhotics by 1 minute (403±43 versus 158±38 μU/ml; P〈0.001) and remained so for the 60 minutes of study. Similarly, after arginine cirrhotics had significantly higher peripheral insulin concentrations and lower portal concentrations than controls. Peak portal vein insulin levels were delayed in cirrhotics (168±16 μU/ml at 3 min) compared with controls (413±25 μU/ml at 1 min). In the basal state both portal and peripheral glucagon levels were higher in cirrhotics than control subjects. Unlike in controls, IV glucose did not suppress glucagon secretion in cirrhotic patients. Peripheral plasma glucagon concentrations after arginine were also consistently higher in cirrhotics than controls, but unlike insulin portal venous glucagon levels were also raised (1800±360 pg/ml, cirrhotics; 960±87 pg/ml, controls; P〈0.001; 1 min after arginine infusion). We conclude that insulin secretion is decreased in liver cirrhosis and that the peripheral hyperinsulinaemia observed reflects diminished hormone metabolism. The high plasma glucagon levels observed in cirrhotic patients are the result of pancreatic hypersecretion of glucagon.
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  • 31
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    Diabetologia 16 (1979), S. 275-279 
    ISSN: 1432-0428
    Keywords: Insulin ; receptors ; mouse embryo retina ; Simian virus 40 ; established cell lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The binding of125I-insulin to uncloned and cloned cultures of mouse retinal cells has been investigated. At 15° C, binding of the hormone reached a steady state by 60 min, while at 37° C equilibrium was reached earlier but at a lower level than at 15° C. Porcine insulin, porcine proinsulin and guinea pig insulin displaced labelled insulin in proportion to their known biological potency. A sharp pH dependence of the hormone binding was observed with an optimum at pH 7.8. The dissociation rate of the125I-insulin was increased in the presence of unlabelled hormone, suggesting the existence of negative cooperativity in the insulin-receptor interaction. The availability of established retinal cell lines with insulin receptors should facilitate the study of the insulin-retina interactions in a controlled in vitro system.
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  • 32
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    Calcified tissue international 27 (1979), S. 33-40 
    ISSN: 1432-0827
    Keywords: Chick embryo ; Bone ; Organ culture ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The study describes the ultrastructure of the mineralized portion of chick tibiae from 10 days in ovo to 2 days post-hatch. At 10 days a single mineralized cylinder surrounds the diaphysis. On its outer surface columnar trabeculae join to form ridges parallel to the long axis of the bone. These ridges are covered by another cylinder and form the haversian canals. At 11 days vascular invasion of the marrow cavity occurs and resorption of the endosteal surface begins. This type of periosteal deposition and endosteal resorption is repeated during and subsequent to embryonic development. The mineralized portion of 10-day chick tibiae cultured for 2 days in modified BGJ medium was compared with 10-, 11-, and 12-day tibiae in ovo. Cultured tibiae were similar in length and calcium content to 11-day tibiae in ovo. The form of mineral deposited in ovo and in culture was the same, namely, aggregates of spherical mineral clusters. Differences in culture included the following: (a) few concentric cylinders were deposited as compared with tibiae in ovo; (b) trabeculae were not arranged in rows and ridges in culture; (c) osteocytic lacunae were restricted to bases of trabeculae rather than uniformly distributed as in ovo; and (d) the endosteal surface of tibiae in culture appeared etched.
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  • 33
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    Calcified tissue international 27 (1979), S. 57-64 
    ISSN: 1432-0827
    Keywords: Bone ; Bone formation ; Calcification ; Calcification nodule ; EM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Osteolathyrism has been used as an experimental model for the study of calcification nodules during the mineralization process. Periosteal exostoses developing in osteolathyrism characteristically have spherical basophilic structures (calcification nodules) in the vicinity of developing bone spicules. In thin sections, the nodules were seen scattered between collagen fibers in the intercellular matrix. Collagen fibers did not appear to be present within the nodules but sometimes were packed just outside them. Matrix vesicles were also present in areas of early mineralization. After EDTA decalcification, the majority of the nodules consisted of a fine granular material surrounded by an electron-dense peripheral zone. The peripheral dense zone was occasionally incomplete in small nodules in areas of early mineralization. An electron-dense central area could be observed in the center of the nodules. Evidence has been presented indicating that the calcification nodules arise from smaller mineralization foci, presumably matrix vesicles. The calcification nodules enlarge to approximately 1.0 µm in size, at which point development is slowed or halted allowing the formation of the peripheral dense zone. Although coalescence of nodules was observed, this was more a random event. The further mineralization of the trabeculae was achieved by the calcification of the collagen fibers. The mineralized trabeculae reflected this pattern of nodular and collagenous calcification. It is suggested that this pattern of calcification is characteristic of rapidly developing woven bone.
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  • 34
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    Calcified tissue international 27 (1979), S. 105-108 
    ISSN: 1432-0827
    Keywords: Bone ; Femur ; Strength ; Density ; Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In order to ascertain whether the intrinsic strength of human bone changes with age or not, we have determined the ultimate tensile strength and density of strips of femoral cortical bone. These femora were collected from cadavers varying in age from 13 to 97 years. The results show that both density and intrinsic strength of bone increase up to about the fourth decade of life and then decrease with age. However, the rate of decrease of strength is greater than that of density. This indicates that the density of bone is not the sole determining factor of its strength, and that some other factors play an important part.
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  • 35
    ISSN: 1432-0827
    Keywords: Bone ; Rickets ; Hypophosphatemia ; Microradiography ; D-resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The perilacunar areas of low mineral density in microradiographs from cortical bone of patients with hypophosphatemic (vitamin D-resistant) rickets are not evenly distributed throughout the bone tissue. Their frequency and distribution were determined in bone from 9 patients with this disease. It was found that the lesion was more frequent in haversian bone than in interstitial bone, and along the inner circumference of growing haversian systems as compared with the outer circumference. These findings indicate that the lesion is the result of retarded mineralization, and that mineralization slowly proceeds in these areas as the bone becomes older. A relatively high frequency of the lesion was also found in osteons with an elliptical cross section along the long axis of the ellipse. The cause of the abundance of the lesion at these sites is not clear, but it is possible to explain the uneven distribution in elliptical osteons by assuming an unequal rate of bone formation in these structures.
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  • 36
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    Calcified tissue international 27 (1979), S. 165-169 
    ISSN: 1432-0827
    Keywords: Oxygen uptake ; Bone ; Oxygen tension ; Carbon dioxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The present study was carried out to determine the influence of carbon dioxide and oxygen tension on the respiratory activity of bone cells in mouse calvaria in vitro. Five-day-old mouse calvaria were removed aseptically and incubated individually for 1 h at 37° C in a closed reaction chamber containing 1.5 ml of tissue culture medium made up of 60% horse serum in Gey's solution containing 100 unit/ml penicillin and 100µg/ml streptomycin. Before the calvaria were added, the medium in the incubation chamber was equilibrated with 10%, 20%, 30%, or 50% oxygen balanced with nitrogen. The effect of CO2 on oxygen utilization by the calvaria was determined by incubating the calvaria in a medium previously equilibrated with either 50% O2 balanced with N2 or 50% O2 and 5% CO2 balanced with N2. At each oxygen tension, the rate of oxygen utilization by the calvaria was measured polarographically by a Clark oxygen electrode. The results showed that the rate of oxygen uptake of bone increased as the oxygen tension increased and carbon dioxide stimulated significantly the rate of oxygen utilization by the bone cells. In view of the previous reports that both carbon dioxide and oxygen tension are implicated in the process of bone resorption, it is suggested that these two factors may affect bone resorption by influencing the oxygen utilization by bone cells and ultimately controlling their energy metabolism.
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  • 37
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    Calcified tissue international 28 (1979), S. 37-42 
    ISSN: 1432-0827
    Keywords: X-ray diffraction ; Hydroxyapatite ; Whitlockite ; Fish (Pisces) ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Pyrolyzed scales, fin spines, and bone from the ray-finned bony fishPolypterus (Actinopterygii) showed two mineral phases on X-ray diffraction: hydroxyapatite (HA), Ca5(PO4)3OH, and whitlockite, Ca3(PO4)2. The ratio of HA/whitlockite varied with the structure (scale, spine, bone) within each individual fish. The relative proportions of HA to whitlockite in pyrolyzed samples reflected the Ca/P ratio of the sample. Whitlockite appears after pyrolysis when the Ca/P is lower than 1.67. Among the five fish investigated, for each structure a general trend was noted. The proportion of HA relative to whitlockite increased with size (age) of the fish. Thus the smallest fish, a juvenile, exhibited a low Ca/P mineral in its calcified tissues, whereas the larger fish had progressively more HA and less whitlockite.
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  • 38
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    Calcified tissue international 28 (1979), S. 17-22 
    ISSN: 1432-0827
    Keywords: Anticonvulsant ; Ketogenic diet ; Calcium ; Vitamin D ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Vitamin D and mineral metabolism status was examined in five children maintained chronically on combined ketogenic diet-anticonvulsant drug therapy (KG), and the results compared to those obtained in 18 patients treated with anticonvulsant drugs alone (AD) and 15 normal controls. KG patients exhibited biochemical findings of vitamin D deficiency osteomalacia: decreased serum 25-hydroxyvitamin D (25OHD) and calcium concentrations, elevated serum alkaline phosphatase and parathyroid hormone concentrations, decreased urinary calcium and increased urinary hydroxyproline excretion, and decreased bone mass. Although the KG and AD groups demonstrated similar reductions in serum 25OHD concentration, the KG patients exhibited a significantly greater reduction in bone mass. In response to vitamin D supplementation (5000 IU/day), mean bone mass in the KG group increased by 8.1±0.9% (P〈0.001) over a 12-month period. These results suggest that ketogenic diet and anticonvulsant drug therapy have additive deleterious effects on bone mass and that these effects are partially reversible by vitamin D treatment.
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  • 39
    ISSN: 1432-0827
    Keywords: Bone ; Bone cells ; Cyclic AMP ; Osteoclast activating factor ; Lymphokine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Osteoclast activating factor (OAF) is a lymphokine which may participate in the pathologic destruction of bone observed in a number of disorders. In the current studies, we investigated the action of OAF on cAMP accumulation by bones and isolated bone cells in culture. OAF was shown to stimulate accumulation of cAMP in mouse cranial bones at doses between 1 and 1000 ng/ml. Stimulation of bone resorption was observed in bones treated with the same doses of OAF. In order to investigate the cell types responsible for cAMP responses to OAF, we isolated bone cells and grew them in monolayer culture. The cells were isolated by a variety of techniques which separate bone cells into two types of parathyroid hormone (PTH)-responsive populations: (a) cells derived from the periosteal regions of the bone, which also respond to calcitonin with increases in cAMP; and (b) cells derived from the matrix, which do not respond to calcitonin. OAF caused elevation of cAMP levels in both the periosteum-derived cells and the matrix-derived cells. The magnitudes and time courses of OAF effects in these populations resembled the effects previously reported for PTH in the same populations. OAF stimulated adenyl cyclase in both types of cell populations, but did not produce significant changes in cAMP phosphodiesterase activity. OAF differed from PTH in that its effects on cAMP accumulation decreased sharply at supramaximal doses in both bone and isolated cells, especially in the matrix-derived populations. Bone resorption did not decrease as markedly as did cAMP accumulation at high doses of OAF, suggesting that cAMP accumulation and resorption could be dissociated under some conditions. These results indicate that OAF has effects on cAMP production in the same cell populations as PTH, and suggest that OAF could modify not only resorption but also formation of bone in vivo. OAF may exert its effects on bone by means of cAMP-dependent mechanisms, but more data will be necessary to establish this unequivocally. The observed differences between OAF and PTH may be of relevance in the mechanism and treatment of pathologic bone destruction in vivo.
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  • 40
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    Calcified tissue international 27 (1979), S. 199-204 
    ISSN: 1432-0827
    Keywords: Bone ; Parathyroid hormone ; Calcitonin ; Osteoporosis ; Paraplegia ; Hydroxyproline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In paraplegia, osteoporosis below the neurological lesion occurs early after the spinal cord affection. The serum levels of parathyroid hormone (PTH) and calcitonin (CT), using a radioimmunoassay for the measurement of immunoreactivity, were studied in 12 paraplegic patients for 9 months following onset. Serum Ca and P levels, urinary hydroxyproline excretion, and the kinetic metabolic clearance of45Ca have also been measured. P and immunoreactive (i) CT levels were found the highest at the beginning of the observation and progressively decreased with time. Ca and iPTH serum levels varied inversely with time, the highest level of Ca and the lowest level of iPTH being recorded at the third month following the paraplegia. Mean values of Ca, iPTH, and iCT were in the normal range throughout the study. P levels were increased during the first 3 months. Hydroxyprolinuria was also high and45Ca kinetics showed increased values of Vt, Vo+, and Vu. These parameters indicate a high degree of bone turnover. The results were consistent with the assumption that PTH is not responsible for the increased resorption of bone in paraplegia. Likewise, a deficiency of CT does not seem to be responsible for this bone resorption. These endocrine modifications could be secondary to an increase in the calcium flux from bone to blood and resulting from bone destruction as attested by the increase of urinary calcium and urinary hydroxyproline excretion.
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  • 41
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    Calcified tissue international 27 (1979), S. 233-237 
    ISSN: 1432-0827
    Keywords: Bone ; Vitamin D ; Acidosis ; Phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Vitamin D and phosphate deficiency were produced in rats in order (a) to evaluate the degree of bone mineral and matrix maturation using a bromoform/toluene density gradient technique; and (b) to compare the aforementioned bone maturational changes due to vitamin D and phosphate deprivation to those produced with superimposed severe acidosis. Rats were fed a diet deficient in vitamin D and phosphorus (0.2%) from 3 weeks through 7 weeks of age. To examine the additional contribution of dietary calcium, we gave one-half of the animals either a low (0.06%) or high (1.3%) calcium diet. Following the 4 weeks of vitamin D deficiency, one-half of each group was given 1.8% NH4Cl in the drinking water for 4 succeeding days to induce an acute, severe acidosis. The degree of bone maturation was quantitated via bromoformtoulene density gradient fractionation; total mineral and hydroxyproline (collagen) levels were quantitated as well. The vitamin D-deficient rats deprived of adequate dietary phosphate responded by conserving phosphorus, and as a consequence total bone phosphorus levels were maintained within that level for control rats. This conservation was independent of calcium intake but was extremely sensitive to acute acid loading, where a significant reduction in total bone phosphorus was noted. The bone maturational profile obtained from the vitamin D-phosphate deficient rats, however, revealed a significant accumulation of less mature or dense bone collagen and mineral with a corresponding decrease in the most mature or dense moieties. In contrast to the reduction of the total bone phosphorus content by acute acidosis, the skeletal collagen-mineral maturational profile was not significantly affected by the short-term systemic acidosis. The observed retardations in the bone collagen and mineral maturation of the vitamin D-deficient, phosphate-deprived state provide an additional observation which may well relate to the progressive osteopenia documented in states of chronic, mild acidosis.
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  • 42
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    Calcified tissue international 27 (1979), S. 275-279 
    ISSN: 1432-0827
    Keywords: Proline ; Cartilage ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Proline biosynthetic and degradative enzymes are unevenly distributed in differentiated mammalian tissues. Activities of the synthetic enzymes are relatively high in collagenous tissues, whereas activities of the degradative enzymes are high in noncollagenous tissues. In order to further characterize tissue-specific proline biosynthesis and degradation, we have determined proline enzyme activities during cartilage and bone formation induced by demineralized bone matrix. We can thus follow temporal changes in enzyme activity in a single tissue as different cell types develop. Ornithine aminotransferase and pyrroline-5-carboxylate reductase have peaks of activity which correlate with maximal type II collagen synthesis by chondrocytes. Both enzymes also are active during bone formation. In contrast, proline oxidase and pyrroline-5-carboxylate dehydrogenase are present at low levels and do not change as new cell types appear. Arginase activity peaks during the first 3 days and then rapidly decreases by the time cartilage and bone formation begin. These observations further substantiate the importance of proline biosynthesis in collagenous tissues. The close correlation between ornithine aminotransferase activity and type II collagen synthesis suggests that the pathway from ornithine to proline may be especially important during formation of type II collagen.
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  • 43
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    Calcified tissue international 27 (1979), S. 109-115 
    ISSN: 1432-0827
    Keywords: Bone ; Bending rigidity ; Disuse atrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The in vivo bending rigidity and bone mineral content of monkey ulnae and tibiae were measured. Bending rigidity in the anteroposterior plane was measured by an impedance probe technique. Forced vibrations of the bones were induced with an electromechanical shaker, and force and velocity at the driving point were determined. The responses over the range of 100–250 Hz were utilized to compute the bending rigidity. Bone mineral content in the cross section was determined by a photon absorption technique. Seventeen male monkeys (Macaca nemestrina) weighing 6–14 kg were evaluated. Repeatability of the rigidity measures was 4%. Bone mineral content was measured with a precision of 3.5%. Bending rigidity was correlated with the mineral content of the cross section,r=0.899. Two monkeys were evaluated during prolonged hypodynamic restraint. Restraint produced regional losses of bone most obviously in the proximal tibia. Local bone mineral content declines 17 to 24% and the average bending rigidity declines 12 to 22%. Changes in bones leading to a reduction in mineral content and stiffness are discussed.
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  • 44
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    Calcified tissue international 27 (1979), S. 13-18 
    ISSN: 1432-0827
    Keywords: Anticonvulsants ; Calcium ; Vitamin D ; Parathyroid hormone ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Parameters of mineral metabolism were examined in 6 patients with moderately severe anticonvulsant drug-induced osteomalacia. Compared to 15 matched controls, the patients exhibited significantly reduced serum calcium, inorganic phosphate, and 25-hydroxyvitamin D concentration, elevated serum alkaline phosphatase and immunoreactive parathyroid hormone (iPTH) concentration, reduced intestinal47Ca absorption, reduced urinary calcium and increased urinary hydroxyproline excretion, and reduced forearm bone mass. Intestinal absorption of vitamin D3 was normal. Following 4 months of treatment with vitamin D3 (4000 units/day), serum 25-OHD concentration was increased to 3 times mean normal values and all parameters except serum iPTH, urinary calcium excretion, and forearm bone mass were returned to levels not significantly different from normal. Serum iPTH concentration was reduced by 39% (P〈0.05); 24-h urinary calcium excretion rose by 98% (P〈0.001), and forearm bone mass increased by 5.6% (P〈0.05). It is concluded that moderate-dose vitamin D3 supplementation is effective in normalizing parameters of mineral metabolism in this disorder, despite evidence of resistance to the biologic effects of vitamin D.
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  • 45
    ISSN: 1432-0827
    Keywords: Calcium ; PTH ; 1,25(OH)2D ; Acid ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We measured mineral and acid balances, serum iPTH, urinary cAMP/creatinine, and plasma concentrations of 25OHD and 1,25(OH)2D in 7 healthy adults during control conditions and during increased fixed acid production achieved either by the administration of NH4Cl (N=3) or by increased dietary protein intake (N=4). When acid production was increased, the subjects were in positive acid balance and negative Ca balance because of increased urinary Ca excretion. Serum iPTH fell slightly but urinary cAMP and the plasma levels of vitamin D metabolites did not change. We conclude that the accelerated skeletal and urinary losses of Ca that occur when fixed acid production is increased are not contributed to nor compensated for by the parathyroid-vitamin D endocrine systems.
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  • 46
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    Calcified tissue international 29 (1979), S. 169-171 
    ISSN: 1432-0827
    Keywords: Glycosaminoglycans ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Compact bone tissue chondroitin sulphate, previously considered to be pure chondroitin-4-sulphate, was isolated from three adult human femoral diaphyses and digested with chondroitinase ABC. Assaying these digests by means of high performance liquid chromatography we could demonstrate that 12 to 14% of the disaccharide residues were 6-sulphated. The 4/6-ratios were also studied in chondroitin sulphate fractions of different molecular size. Slightly increasing amounts of 6-sulphated disaccharides were found with increasing size of the chondroitin sulphates.
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  • 47
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    Calcified tissue international 29 (1979), S. 7-13 
    ISSN: 1432-0827
    Keywords: Bone ; Culture ; Resorption ; Acidosis ; Alkalosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We have examined the effects of H+, CO2, and HCO3 − concentrations during metabolic and respiratory acidosis and alkalosis on bone resorption in vitro. Rat fetal bones prelabeled with45CaCl2 were cultured at 2%, 5%, and 10% CO2 for up to 120 h, and the release of45Ca was measured in devitalized bones (non-cell-mediated45Ca release) and in live bones (cell-mediated45Ca release) cultured with or without PTH and 1,25(OH)2D3. Non-cell-mediated mineral loss was linearly related to H+ concentration but not to CO2 or HCO3 − concentration. This effect was observed on both labeled and stable calcium. Over a wide pH range (6.9–7.5) H+, CO2, or HCO3 − concentrations did not influence cell-mediated bone resorption in control or in PTH-and 1,25(OH)2D3-stimulated cultures. However, inhibition of cell-mediated bone resorption was observed at higher or lower pH irrespective of CO2 or HCO3 − concentrations. These observations demonstrate that the bone mineral mobilizing effect of acidosis in vitro is mainly due to the effect of changing H+ concentration on devitalized bone. Effects on cell-mediated bone resorption and hormonal response were observed only at extremes of pH. The effects of H+ were independent of changes in CO2 or HCO3 − concentration and could be responsible for the negative calcium balance and increased urinary loss observed in metabolic acidosis in vivo, but do not explain the reported differences in effects on calcium metabolism between respiratory and metabolic acidosis.
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  • 48
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    Experimental brain research 34 (1979), S. 197-200 
    ISSN: 1432-1106
    Keywords: Claustrum ; Visual cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Visual cortex, including areas 17, 18, and sometimes 19, was injected with tritiated leucine. Terminal labelling could be detected by autoradiography in the dorsocaudal part of the ipsilateral claustrum in all cases.
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  • 49
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    Experimental brain research 34 (1979), S. 241-261 
    ISSN: 1432-1106
    Keywords: Locomotion ; Central generator ; Cat ; Deafferentation ; Spinal cord
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A central network of neurones in the spinal cord has been shown to produce a rhythmic motor output similar to locomotion after suppression of all afferent inflow. The experiments were performed mainly in acute spinal cats (th. 12), which had received DOPA i.v. and the monoamine oxidase inhibitor Nialamide. In some preparations all dorsal roots supplying the spinal cord were transected, in others phasic afferent activity was suppressed by curarization. The activity was recorded as neurograms from nerve filaments or as electromyograms. It is concluded that: 1. alternating activity between flexors and extensors of foot, ankle, knee, and hip of one limb can still occur 2. the duration of the flexor discharges vary less with the cycle duration than the extensor discharges 3. different flexor muscles may retain individual patterns 4. the activity at different joints can be dissociated 5. there is at least one network for each limb 6. the coordination between the two hindlimbs can be alternating as in walking or be more closely spaced as in galloping 7. alternating activity in the ankle remains even when only segments L6, L7 and S1 are intact.
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  • 50
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    Pflügers Archiv 382 (1979), S. 51-55 
    ISSN: 1432-2013
    Keywords: Cerebellectomy ; Vestibuloocular reflex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of cerebellectomy on the semi-circular canal evoked responses recorded from individual vertical and oblique eye muscles were studied in cats anesthetized with Ketamine. The phase lag relative angular acceleration of the electromyographic response was consistantly smaller over the frequency range tested (0.02–1.0 Hz) in cerebellectomized than in intact animals. This finding indicates that the time constant of the central, neural integrator was shifted towards smaller values by the lesion. This was also suggested when the vertical eye movements generated by rotation about the pitch axis were recorded in the fully alert animal. In addition, the EOG data show that the phase of the VOR in the low frequency range was not altered following cerebellectomy suggesting that the macular influences were still present. When the EMG responses of the superior oblique (SO) and superior rectus (SR) muscles were studied in their presumed main mode, i.e. roll-canal and pitch-canal, respectively, no difference was noted in hemicerebellectomized animals when compared to intact control animals. However, when SO and SR were studied in pitch-canal and roll-canal rotation, respectively, dramatic changes in the response pattern were noted in lesioned animals.
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  • 51
    ISSN: 1432-2013
    Keywords: Adrenergic innervation ; Endocrine pancreas ; Insulin ; Exercise ; Glucagon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The normal depression of plasma insulin concentration during exercise has been ascribed to adrenergic inhibition of insulin release and the role of humoral catecholamines in this hormonal adjustment has repeatedly been stressed. In the present study this contention has been investigated in 6 bilaterally adrenalectomized patients and in 6 sex- and age-matched controls who undertook exercise on an ergometer until they were exhausted. No differences were observed in any cardiovascular or metabolic adjustments between the two groups during strenous exercise. Mean plasma insulin concentration fell by about 50% in both groups. Phentolamine effectively abolished the fall in plasma insulin concentration during exercise in 2 adrenalectomized patients. The results suggest that the adrenergic nerves that supply the B-cells have a functional role in man during exercise.
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    European journal of pediatrics 132 (1979), S. 189-195 
    ISSN: 1432-1076
    Keywords: First feed ; Newborn ; Insulin ; Glucagon ; C-peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Blood glucose, insulin, C-peptide and glucagon were evaluated in 36 newborn term infants at birth, and before and 60 min after the first feed during the first day of life. Under basal conditions glycaemia diminished during the first day of life and glucagon increased, while insulin and C-peptide did not show any variation. The C-peptide: insulin molar ratio was higher in the newborn than in adults because of the longer half-life of C-peptide, probably due to reduced renal function in the neonatal period. The subjects were divided into two groups: 18 newborn infants were given a feed of commercially available milk powder reconstituted in water at 10% (5 ml/kg); the other 18 were given a feed of 5 ml/kg 10% glucose solution. In each group 6 were given the first feed after a fast of 6 h, 6 after a fast of 12 h, and 6 after a fast of 24 h from birth. After the first feed with milk, the average increase of glycaemia was 19.83 mg%, of insulin 6.06 μU/ml, and of C-peptide 1.88 ng/ml. After the first feed with glucose the average increase of glycaemia was 13.59 mg%, of insulin 2.46 μU/ml, and of C-peptide 0.59 ng/ml. Glucagon did not show significant changes after the first feed.
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    European journal of pediatrics 131 (1979), S. 185-198 
    ISSN: 1432-1076
    Keywords: Growth hormone ; Somatomedin ; Chronic renal failure ; Uraemic children ; Haemodialysis ; Insulin ; Arginine ; Carbohydrate intolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Plasma growth hormone (GH) concentrations after insulin and arginine stimulation were estimated in 11 dialyzed and 6 non-dialyzed children with chronic renal failure. Twenty healthy children served as controls. Plasma GH peak concentration and estimation of the total area under the plasma GH concentration-time curve by the trapezoidal rule were used to evaluate results. Elevated basal GH levels and an exaggerated response to the stimuli were seen in several of the patients. The causes of the abnormal GH secretion and the role of high GH levels in carbohydrate intolerance are discussed. No consistent pattern was seen in GH secretion during haemodialysis without glucose in the dialysate. In children undergoing haemodialysis with a fluid containing glucose, plasma GH fell considerably.
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  • 54
    ISSN: 1432-1440
    Keywords: Papillary muscle ; Hypoxia ; Glucose ; Insulin ; Force of contraction ; Papillarmuskel ; Kontraktionskraft ; Hypoxie ; Glucose ; Insulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die minimal verbleibende Kontraktionskraft unter dem Einfluß von Hypoxie wurde an isolierten, rechtsventrikulären Papillarmuskeln (Meerschweinchen) gemessen, die kurzdauernd (90 s) mit hypoxischem (pO2 90 mm Hg) statt oxygeniertem (pO2 630 mm Hg) Medium inkubiert wurden. Unter dem Einfluß von Insulin (0,05 IE/ml) bei erhöhter Glucosekonzentration (300 mg% im Vergleich mit 100 mg%) sank die Kontraktionskraft minimal nur auf 70±9% im Vergleich mit 38±11% des Ausgangswertes (Kontrolle,p〈0,001). Eine alleinige Erhöhung der Glucosekonzentration oder Zugabe von Insulin hatte geringere Effekte zur Folge. Diese Befunde dokumentieren eine erhebliche Steigerung der Hypoxietoleranz des Myokards durch Glucose-Insulin.
    Notes: Summary The minimally remaining force of contraction under the influence of hypoxia was measured in isolated right-ventricular papillary muscles of the guinea-pig, incubated in hypoxic (pO2 90 mm Hg) and oxygenated (pO2 630 mm Hg) buffer solution. With insulin (0.05 IE/ml) and elevated glucose (300 mg% compared to 100 mg%) the force of contraction fell to only 70±9% minimally instead of 38±11% (control,p〈0,001). Elevated glucose or insulin alone had less pronounced effects which were not statistically significant for insulin. The results show an increase of tolerance against hypoxia of isolated papillary muscles under the influence of glucose and insulin.
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    Psychopharmacology 63 (1979), S. 151-153 
    ISSN: 1432-2072
    Keywords: Discriminative stimulus properties ; Psychomotor stimulants ; Cat ; d-Amphetamine ; Clozapine ; Cocaine ; Morphine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cats were trained to choose between two levers of an operant chamber using interoceptive cues provided by d-amphetamine or saline as the discriminative stimuli. Following training, stimulus generalization was observed to additional doses of d-amphetamine and cocaine, but not to morphine. Clozapine blocked the generalization of the drug discrimination response to d-amphetamine, but had no effect on generalization to cocaine. These data indicate that discriminative stimulus properties of psychomotor stimulants, previously described in rats, are similar in cats.
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  • 56
    ISSN: 1432-0878
    Keywords: Thalamus ; Horseradish peroxidase ; Golgi-impregnation ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Afferent connections to the ventrobasal complex (VB) of the thalamus were studied by means of retrograde transport of horseradish peroxidase (HRP) and by the Golgi-method. After HRP-injection into the VB, peroxidase-positive cells were observed contralaterally in the dorsal column nuclei (DCN), in the trigeminal nuclei and in the lateral cervical nucleus (LCN), and ipsilaterally in the somatosensory I (SI) and II (SII) cortical areas. Labeled cells of different shape and size were compared with neurons impregnated by the Golgi-technique. On the basis of HRP-labeling it is concluded that cells projecting to the VB are different in size and shape even within one region and that they correspond to the relay or efferent neurons observed in the Golgimaterial.
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    European journal of applied physiology 41 (1979), S. 51-59 
    ISSN: 1439-6327
    Keywords: Exercise ; Training ; Free fatty acids ; Triglycerides ; Insulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Twenty healthy athletes performed a heavy physical exercise before and after a controlled training period of 3 months. As a result of physical training there was a reduction in lactate concentration during and after exercise. Plasma free fatty acids and triglyceride levels were lower at rest as well as during and after exercise. Insulin concentrations decreased during exercise before the training period whereas they remained constant afterwards. The composition of individual free fatty acids changed in the same way during exercise before and after training: fatty acids with shorter hydrocarbon chains increased, those with longer chains decreased. Comparing the pattern of individual free fatty acids before and after training a higher percentage of saturated and a lower percentage of mono-unsaturated fatty acids was observed. It is concluded that changes in the plasma free fatty acid profile during heavy exercise reflect a preferential uptake and oxidation of certain individual free fatty acids. The significance of training-induced changes in the plasma free fatty acid pattern is discussed.
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    European journal of applied physiology 41 (1979), S. 211-219 
    ISSN: 1439-6327
    Keywords: Adrenaline ; Noradrenaline ; Glucagon ; Insulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of 9 weeks of training on responses of plasma hormones to swimming were studied in eight competitive swimmers who had not trained for several months. Two types of swimming tests were used: (1) 200 yd, a high intensity, exhausting type of exercise in which maximal effort was required both before and after training, and (2) 1000 yd, a pace type of exercise in which subjects swam as fast as possible prior to training and at the same rate after training. Plasma levels of glucagon increased and of insulin decreased during 1000 yd of swimming, but were not altered by 200 yd of swimming. No training effects were apparent in responses of plasma insulin and glucagon to these short-term, high intensity exercise tests. During the 1000 yd swim, plasma adrenaline was 0.8 ng/ml before vs. 0.1 ng/ml after training. Plasma noradrenaline response decreased from 3.4 to 1.2 ng/ml as a result of training. In the 200 yd swim, adrenaline, but not noradrenaline, was lower after training.
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    European archives of oto-rhino-laryngology and head & neck 222 (1979), S. 113-118 
    ISSN: 1434-4726
    Keywords: Brain-stem electric response (BSER) ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The early auditory evoked responses have been studied in bilaterally hearing cats, and in animals with hereditary unilateral anacusis. Cross-over stimulation of the hearing ear occurs at an interaural attenuation of 50-50 dB, and all six waves of the responsare present at supra-threshold stimulus levels.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 1851-1863 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The boundary control problem of optimal heating of an infinitely long slab with tempertue-dependent thermal conductivity, subjected to a convection and radiation boundary condition, is analysed by numerical methods.In order to reformulate the optimal control problem of distributed parameter systems as a mathematical programming problem of finite dimension, a space, co-ordinate is discretized by use of the finite element method, while the Runge-Kutta method is utilized for time integrations. Finally, the performance index of the optimal control problem is minimized by the conjugate gradient method of optimization.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 1879-1879 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 37-50 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 11-35 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Finite element equivalents of the equations governing shearing and buoyancy driven flows are derived, and reduced to upwind forms suitable for the solution of problems in which the Reynolds and Rayleigh numbers are large. A modification to the central difference iterative method is studied which increases the Reynolds and Rayleigh numbers for which a central difference form may be used. A comparison is made between the results obtained using the central and upwind forms of the finite element method and those predicted by finite difference methods in the case of flow in a cavity. A mesh refinement study is made. The upwind forms of the finite element equations are applied to the solution of a complex flow problem involving the flow of glass in a throated furnace in the case of constant- and temperature- dependent viscosity and conductivity.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 51-68 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The 18 degree-of-freedom flat triangular shell element is reformulated by combining the well-known bending triangle with a plane stress triangle incorporating in-plane rotations at each vertex. Both elements are displacement formulated. The plane stress element's displacement interpolation is incomplete and hence convergence to exact solutions is precluded. Comprehensive test results are presented for several types of problem including plane stress, thin shells and folded plates. The results indicate that the element does produce rapidly convergent answers. However these answers are not the correct ones, although they may be acceptable engineering approximations in many applications. Further, the element seems to provide reasonably good results even for relatively coarse element grids.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 81-102 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The paper considers the finite element analysis of the free, undamped and the forced damped vibrations of rotationally periodic structures. Associated with every natural frequency (except for those for which the deflection is the same at corresponding points on every substructure) there are a pair of orthogonal mode shapes, with eigenvectors {u} and {ü}. The complex vector {z}={u}+i{ü} is also an eigenvector of the equations of motion, and represents a rotating normal mode. The deflection of one substructure has the same amplitude as, and a constant phase difference from the deflection of the preceding substructure. It is therefore possible to analysse the complete structure by considering only one substructure, and applying appropriate complex constraints at its boundary with the following substructure, so as to and applying appropriate complex constrainsts at its boundary with the following substructure, so as to impose this phase difference. The method has been implemented in a computer program and is illustrated by analyses of an alternator end winding, a cooling tower with legs, and a wheel of turbine blades.For forced vibration, it is shown that any arbitrary oscillatory force can be decomposed into a series of rotating forces. For any one of these rotating components, there is a fixed relationship between the amplitude and phase of the force acting on one substructure, and that acting on an adjoining substructure. This relationship, which does not involve any approximation, can be used to enable a series of calculations of the response of one substructure to be performed instead of one on the whole structure. A series of calculations on an individual substructure normally requires much less computer time and storage than a single calculation on the complete structure.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 115-124 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Load-free notch surfaces of bodies made of a linear-elastic, homogeneous, isotropic material are optimized within given variation domains by means of an interation procedure so that the occurring maximum tangéntial stress is reduced to a minimum. The hypothesis of a constant tangential stress distribution for obtaining minimal notch stresses proposed by R. V. Baud has been confirmed by means of the Fade-away Law of the notch stress theory. The finite element method is applied for calculating the displacement and stress field of the styructure. An increment procedure is used for determing the displacement field after every iteration step, which permits the calculation of the displacement quantities with sufficient accuracy in a single step. Numerical solutions and their comparison with analytical solutions and stress results obtained with the aid of photo-elasticity confirm the usefulness of this procedure. The known optimization methods1,2 differ from those used in the present paper.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 317-336 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper presents a novel integral-equation technique for solving the steady-state wave-resistance problem. The free-surface condition is linearized, but the body condition is satisfied exactly. An integral relation describing the flow inside an arbitrarily truncated internal region is first obtained by applying Green's Theorem, using only the simple source function for an infinite fluid. The internal flow is next matched with eigen expansions in the upstream and downstream outer regions. The radiation condition can be satisfied exactly simply by a proper choice of the solution form in these outer regions. The method is applied to investigate flows about both lifting and non-lifting two-dimensional bodies. Agreement with existing results is excellent. The present formulation provides a simple yet rational basis for tackling the practical three-dimensional ship-wave problem, in which past workers have encountered considerable difficulties using a complicated free-surface Green function.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 379-408 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper we present an algorithm for adaptively computing smooth piecewise polynomial approximations using restricted range uniform approximations on a finite point set. We also present several numerical examples and offer suggestions for the effective use of this algorithm. We have found the algorithm to be effective for approximating a wide class of functions, either with or without significant levels of noise. Furthermore, since the user of this algorithm actually defines tolerance bands within which the approximation will lie, the algorithm allows the user a great deal of flexibility and control over the shape of the resulting approximations.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 337-357 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The use of contacting components such as gear teeth in mesh and shrink-fitted shafts is very common in engineering practice. This paper deals with the development of a theoretical method which gives a solution for non-linear contact problems with irreversibility resulting from stick-slip phenomenon.The method is based on the finite element method and load incremental theory. The geometrical and the statical boundary conditions on contact surfaces are treated as additional conditions being independent of stiffness equations. As a result, the algorithm of calculation is simplified and only a part of the simultaneous equations related to the contact surfaces is required to be solved instead of the overall stiffness equations at each step. Furthermore, the magnitude of load causing a change in a contact condition of one contact nodepair is taken as a load increment, in analogy with the incremental iterative procedure for elastic-plastic problems. Therefore, the method provides a general and efficient method for analysis and design of such problems.As illustrative examples, the stick-slip behaviour of turbo-alternator end-bells and other problems are discussed. The calculated results show a reasonable agreement with experimental data and other solutions.
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    International Journal for Numerical Methods in Engineering 14 (1979) 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 507-513 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 499-505 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The method for the evaluation of the thermal loads used in the incremental finite element analysis of elasto-plastic stress is presented. The stress distributions obtained by two apparently possible methods for evaluation of the thermal loads are compared.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 475-497 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: This paper presents an isoparametric finite element formulation for the torsion and the flexure due to end shears for the beam cross-sections of arbitrary shape. Isoparametric line elements are developed using this formulation for the beam cross-sections consisting of very thin wall open or close multicells. Isoparametric transition elements are also developed for the beam cross-sections consisting of both thin wall sections and solid like sections. Numerical examples are presented to demonstrate the accuracy and the applications of such elements.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 515-529 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new element stiffness matrix is derived for straight cable elements subjected to tension and torsion. The cross-section of a cable, which may consist of many different structural components, is treated in the following as a single composite element. The derivation is quite general; consequently, the results can be used for a broad category of cable configurations. Individual helical armourning wires, for instance, may have unique geometric and material properties. In addition, no limit is placed on the number of wire layers. Furthermore, compressibility of the central core element can also be considered. The equations of equilibrium are first derived to include ‘internal’ geometric non-linearties produced by large deformations (axial elongation and rotatioin) of a straight cable element. These equations are then linearized in a consistent manner to give a liner stiffness matrix. Linear elasticity is assumed throughout. Excellent agreement with experimental results for two different cables validates the correctness of the analysis.
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  • 75
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 531-544 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A high precision triangular thick orthotropic plate bending element on an elastic foundation is developed for the free vibration analysis of thick plates on elastic foundation. The element has three nodes with twelve degrees-of-freedom per node, and takes into account the shear deformation and rotatory inertia. The accuracy of the element is established by comparison of the natural frequencies of certain thick and thin plates, determined from a consistent mass matrix formulation, with available results.
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  • 76
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 545-565 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A finite element simulation of the equations of momentum and energy transport in fluids has been implemented with triangular elements. An attempt is made to single out the reasons for numerical instabilities reported by other investigators for convection-diffusion transport operations in fluid mechanics when the ratio of the convective to the diffusive terms, measured by the Reynolds and Peclét numbers, is of the order of a hundred. To this end, the equations are solved for several problems to permit a direct comparison with results of other formulations. It is shown that the appearance of instability can be delayed by a proper choice of boundary conditions, and its intensity can be reduced through the use of triangular finite elements. Results agree very well with theoretical solutions for particular test problems including flows with large convection effects, large dissipation effects and fluids with temperature dependent properties.
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  • 77
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 567-580 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Methods are described and reviewed for the accurate numerical evaluation of improper integrals encountered in conformal transformation solutions involving boundaries of relatively complicated shape. Four methods are reviewed for the solution of integrals containing end-point singularities. Two new methods are discussed for the solution of integrals containing both end point singularities and simple poles within the range of integration. One method uses a combination of a simple recursive formula and the coefficients of a Chebyshev series and a second method involves subtracting out the singularity and the use of Gauss-Jacobi quadrature. Both methods can give results of high accuracy and an upper limit of the error is readily found. A numerical example is taken which is typical of the application to practical problems and this brings out a comparison of the two methods.
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  • 78
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 633-645 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper present an algorithm and its corresponding FORTRAN IV program which solves a system of simultaneous equations of the type generated in structural analysis. It is based on the Gauss elimination method, so it is a direct method. But it only needs to have in core as many equations as it can contain, with a minimum of two. The program offers the following advantages: (a) Takes into account zeros to avoid operations. (b) Considers very big numbers in the diagonal, corresponding to boundary conditions, to avoid operations. (c) It can handle several load cases either simultaneously or in groups, at choice.
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  • 79
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 786-786 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 80
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 787-790 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 81
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 785-786 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 82
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    International Journal for Numerical Methods in Engineering 14 (1979) 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 83
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    International Journal for Numerical Methods in Engineering 14 (1979), S. i 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 84
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    International Journal for Numerical Methods in Engineering 14 (1979), S. ii 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
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  • 85
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 791-812 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The essence of the proposed curvilinear finite difference solution method is the direct solution of the transformed fourth order partial differentiall equation using the finite difference expressions based on the flexible structure so that the geometry generality of the finite element method can be approached. The examples of applications indicte that the curvilinear mesh can be used and the boundary condition on the curved boundary can be easily and accurately prescribed.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 813-836 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An interactive computer graphics system has been developed for generating the geometric description of an arbitrary shell surfface. The various difficulties facing the user of sophisticated, doubly-curved shell finite elements and the inefficiency of preprocessing for shell analysis are discussed to explain the benefits of this system. The method is a synthesis of spline theory and algorithms, and an interactive means for man-machine communication. The basic technique employed is a modified lofting method in which cardinal splines. An inversion procedure is incorporated which increases significantly the efficiency of the B-spline approximation process. Static and dynamic graphic displays greatly enhance visualization at any stage of the procedure. Two examples of practical interest are presented in some detail to demonstratge the flexibility and usefulness of this approach. Some natural extensions of this system for interactive mesh generation on the surface are also demonstrated.
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  • 87
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 837-853 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Three widely-used nodal resequencing algorithms were tested and compared for their ability to reduce matrix profile and root-mean-square (rms) wavefront, the latter being the most critical parameter in determining matrix decomposition time in the NASTRAN finite element computer program. The three algorithms are Cuthill-McKee (CM), Gibbs-Poole-Stockmeyer (GPS), and Levy. Results are presented for a diversified collection of 30 test problems ranging in size from 59 to 2680 nodes. It is concluded that GPS is exceptionally fast, and, for the conditions under which the test was made, the algorithm best able to reduce profile and rms wavefront consistently well. An extensive bibliography of resequencing algorithms is included.
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  • 88
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 855-870 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A simple computer method is presented for analysing statically indeterminate beams having sectionally constant EI values and loads consisting of any number of arbitrarily placed concentrated forces and couples as well as any number of uniform and ramp loads. Unknown reactions are treated as parameters which are determined by simulataneous imposition of end conditions and interior constraints. Support locations are specified independently of any discontinuites of load or cross-section. A computer program implementing this method is described which has very concise data input considerably shorter than tupically required in programs based on the stiffness method.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 871-903 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In References 1 and 2, algorithms were introduced for adaptively computing smooth piecewise polynomial approximations using uniform, least-squars (l2) and restricted-range uniform approximations. This present paper introduces a FORTRAN program for computing smooth piecewise polynomial approximations to data and/or precise mathematical functions (in discrete form) which allows the user the option of using best l1 of best l2 approximations. (The l2 option of this newly developed l1-l2 adaptive curve-fitting package supersedes the code listed in Reference 1 for the old least-squares program.) In this paper, adaptive curve-fitting algorithm used in this l1-l2 package is described in detail, the FORTRAN program is the given (in the Appendix) and discussed, the numerical results are presented in an effort to illustrate how the l1 and l2 versions of this algorithm may be used most effectively.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 905-911 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A numerical experiment has been conducted to check theeffectiveness and reliablity of the orthnormalization process tosolve two-point boundary value problems. This process in usedalong with the method of superposition in the linear case andwith the quasilinearization and then the method of superpositionin the non-linear case.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 921-931 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Truncation errors introduced by operator splittingtechniques, such as the ADI scheme, are discussed in the contextof the two-dimensional shallow-water equations. Numericalexamples of flow along a uniform channel, with and withoutfriction, illustrate the practical significance of these errors when the boundary is not aligned with the computational gridaxes. With irregular boundaries the maximum time step aceptable ismore severely restricted than has been realized hitherto. The problem, which is shown to stem from the treatment of the boundary conditons, is inherent in any splitting scheme and is difficult to circumvent.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 913-920 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Long thin test specimens of orthotropic material are modelledby quadratic isoparametric finite elements in plane strain. Displacement oscillations occur down the long free edge of themodel with both reduced (2 × 2) and full (3 × 3) integration, themagnitude of the oscillations being affected by element aspectratio and the elastic constants of the material. The rate ofdiffusion of stress as predicted by the classical shear-lag theory, being dependent on the material constants, is reflectedin the displacement oscillations near the load in 3 × 3integration. In 2 × 2 integration, however, this effect is swampedby the build-up of oscillations away from the restraints in alowenergy deformation mode which becomes dominant as the elementaspect ration is increased. These latter voscillations arepredicted by the illconditioning of the structural stiffnessmatrix measured by a solution diagonal decay factor.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 937-941 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 933-936 
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    Keywords: Engineering ; Engineering General
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 946-948 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 96
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 942-945 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 3 Ill.
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    International Journal for Numerical Methods in Engineering 14 (1979) 
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  • 98
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 949-959 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A modification of the collocation method for the numerical solution of Cauchy-type singular integral equations appearing in plane elasticity and, especially, crack problems is proposed. This modification, based on a variable transformation, applies to the case when the unknown function of the singular integral equation behaves like A(x - c)α + B(x - c)β, where α 〈 0, 0 〈 β - α 〈 1, near an endpoint c of the integration interval. In plane elasticity such a point is either a crack tip or a corner point of the boundary of the elastic medium. Thus the method seems to be quite efficient for the numerical evaluation of generalized stress intensity factors near such points. A successful application of the method to the classical plane elasticity problem of an antiplane shear crack terminating at a bimaterial interface was also made.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 961-986 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An updated Lagrangian and a total Lagrangian formulation of a three-dimensional beam element are presented for large displacement and large rotation analysis. It is shown that the two formulations yield identical element stiffness matrices and nodal point force vectors, and that the updated Lagragian formulation is computationally more effective. This formulation has been implemented and the resulted of some sample analyses are given.
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    International Journal for Numerical Methods in Engineering 14 (1979), S. 987-1000 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new numerical method of calculating unsteady-state heat conduction and thermal stresses by using improved coefficient matrix patterns on the nodal points of a structure is given. Good agreement is obtained between our results and the rigorous results, and this method seems to yield better accuracy than other well-known approximate methods for two-dimensional transient thermal stress problems.
    Additional Material: 9 Ill.
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