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  • 1980-1984  (1,158)
  • 1983  (1,158)
  • Analytical Chemistry and Spectroscopy  (803)
  • Atomic, Molecular and Optical Physics  (251)
  • Ultrastructure  (104)
  • Nuclear reactions
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Material
Years
  • 1980-1984  (1,158)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Membranous lipodystrophy-like changes in ischemic necrosis of the legs (1983)
    Springer
    Virchows Archiv 399 (1983), S. 191-205 
    Details
    ISSN: 1432-2307
    Keywords: Membranous lipodystrophy ; Fat tissue ; Ischemic necrosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Subcutaneous fat from 3 patients with ischemic necrosis of the legs due to arteriosclerotic obstruction were examined histologically and ultrastructurally. Markedly convoluted membranocystic changes were found in all 3 cases. The light and electron microscopic findings of the membranocystic lesions are very similar to those of fat tissue changes in membranous lipodystrophy. Bone lesions and mental disturbance which suggest membranous lipodystrophy, however, were absent in these cases. It is concluded from these results that the membranocystic changes characteristic of membranous lipodystrophy can be produced by circulatory disturbance and the lesions are one of the non-specific changes of adipose tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00619579
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  • 2
    Electronic Resource
    Electronic Resource
    Mammosomatotroph cell adenoma of the human pituitary: a morphologic entity (1983)
    Springer
    Virchows Archiv 398 (1983), S. 277-289 
    Details
    ISSN: 1432-2307
    Keywords: Pituitary adenoma ; Ultrastructure ; Immunocytochemistry ; Acromegaly ; Hyperprolactinemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Nine cases of a hitherto undescribed morphologic entity, termed mammosomatotroph cell adenoma of the human pituitary, are reported. These tumors, occurring mostly in men, are invariably associated with acromegaly (or gigantism) and high-normal or slightly elevated blood prolactin levels, and it cannot be distinguished clinically from well-differentiated growth hormone cell or mixed growth hormone cell-prolactin cell adenomas. They show a slow growth rate and usually exhibit a diffuse pattern and intense cytoplasmic acidophilia by histology. The immunoperoxidase technique detects both growth hormone and prolactin within the same cells. Electron microscopy reveals monomorphous tumors with a fine structure markedly similar to that of well-differentiated, densely granulated growth hormone cell adenomas. An added feature and diagnostic marker of mammosomatotroph cell adenoma is the presence of extracellular deposits of secretory material. One tumor shows a marked abnormality of hormone packaging and storage, resulting in the cytoplasmic accumulation of pleomorphic bodies containing semicrystalline secretory material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583585
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  • 3
    Electronic Resource
    Electronic Resource
    A morphological study of the changes which occur during pregnancy in the human breast (1983)
    Springer
    Virchows Archiv 401 (1983), S. 163-175 
    Details
    ISSN: 1432-2307
    Keywords: Breast ; Human ; Pregnancy ; Ultrastructure ; Morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In this study the structural changes which occur during human pregnancy were examined by light and electron microscopy. Pregnancy was associated with proliferation and differentiation of the epithelial cells within the lobules. Proliferation was continuous throughout pregnancy with a progressive increase in the size of the lobules. The highest level of mitosis was observed in the first trimester with lower levels in the second and third trimesters. Unexpectedly a number of apoptotic cells were observed during pregnancy. Differentiation was initiated in the second trimester with an increase in the amount of rough endoplasmic reticulum and the appearance of a hypertrophic Golgi body and lipid droplets within a number of epithelial cells. A number of small vacuoles were present close to the apical plasmalemma of a few epithelial cells. As the pregnancy proceeded there was an increase in the number of cells exhibiting these features. There was also an increase in the size of the lipid droplets and the number of apical vacuoles. The apical vacuoles which have not been described previously range in size from 150–600 nm with the contents of the larger vacuoles having a whorled or labyrinth-like appearance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692642
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  • 4
    Electronic Resource
    Electronic Resource
    Achilles tendon injury (1983)
    Springer
    Virchows Archiv 399 (1983), S. 177-189 
    Details
    ISSN: 1432-2307
    Keywords: Achilles tendon ; Tendon injuries ; Muscles ; Myofibrils ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Unilateral tenotomy of the Achilles tendon was carried out in 60 rabbits. The limb was then either mobilized directly or immobilized for 10 to 35 days using a plaster usually after tendon suture. In certain cases the plaster was removed early (on the 7th or 16th day) and the animals were than allowed to use this leg. Separation between tendon ends was apparent from steel markers, placed close to each cut end of the tendon and examined by X-ray. The separation curve was biphasic and both the first and the inactive phase reflected the degree of tension over the tendon suture. However, during the second separation phase, which began between the 17th and 21st day, the separation gradually reached the same level in all groups. Enzyme histochemistry and electron microscopy revealed severe degenerative changes in immobilized and in shortened muscles. Furthermore, a gradual shift in fibre type characteristics from type 1 slow-twitch fibres towards type 2 fast-twitch fibres occurred. Rapid recovery followed removal of the plaster. The findings indicated that both degenerative and regenerative processes and adaptive processes had been initiated in all experimental muscles when the tendon continuity was broken. The adaptive processes progressed gradually during the five-week post-operative period and might have been responsible for the second phase of the tendon end separation. The fibre adaptation, i.e. the transformation, may be accounted for by changes in structure of the myofibrils and composition of the myosin molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00619578
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructure of the congenital epulis (1983)
    Springer
    Virchows Archiv 401 (1983), S. 251-260 
    Details
    ISSN: 1432-2307
    Keywords: Congenital epulis ; Ultrastructure ; Granular cells ; Intracellular collagen fibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This report presents the ultrastructural features of a congenital epulis. The granular cells of the epulis were packed with numerous membrane bound cytoplasmic granules containing particles, small vesicles, and electron-dense materials. These granules were negative in immunohistochemical reaction for CEA (DAKO PAP KIT). Cytoplasmic organelles such as mitochondria, rough surfaced endoplasmic reticulum, and Golgi apparatus, were absent. Nuclei were markedly indented. Occasionally, banded intracellular collagen fibrils were observed within the cytoplasm. Some of these fibrils were surrounded by a limiting membrane, whereas others appeared to lie free in the cytoplasm. The collagen fibrils were also seen within a deep invagination of the cell surface. There was no basal lamina around the granular cells. Sporadically, mast cells with many granules containing lamellar formations were found between the granular cells. These observations support the idea that granular cells of the congenital epulis are derived from mesenchymal cells, probably fibroblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00734843
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  • 6
    Electronic Resource
    Electronic Resource
    Pituitary hyperplasia (1983)
    Springer
    Virchows Archiv 399 (1983), S. 277-287 
    Details
    ISSN: 1432-2307
    Keywords: Pituitary ; Hyperplasias ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Surgical specimens of 15 normal and 106 para-adenomous anterior pituitaries were studied immunocytochemically and in part electron microscopically for the presence of hyperplasia. GH cell hyperplasia was found in 13% of all normal pituitaries, in 6% of the cases with Prolactin secreting adenomas and in 9% of the cases with ACTH secreting adenomas. Prolactin cell hyperplasia occured in nearly equal percentages (17–23%) in normal pituitaries and in areas adjacent to GH-, Prolactin-or ACTH-secreting adenomas or adjacent to inactive adenomas. Previous findings of relatively more frequent Prolactin cell hyperplasia occuring together with Prolactin producing adenomas have to be revised. Prolactin cell hyperplasia as a primary source of hyperprolactinemia is very rare and almost always occurs in conjunction with oncocytic adenomas. ACTH cell hyperplasia was found in 13% of the normal pituitaries, in 14% of the cases with Prolactin secreting adenomas, in 58% of the cases with ACTH producing adenomas and in 40% of the pituitaries with GH secreting adenomas. We have no explanation for the latter result. ACTH cell hyperplasia may be the primary cause of Cushing's disease (18% of all Cushing cases). Hyperplasia of TSH cells in normal pituitaries was rare (7%) and with the exception of Prolactin producing adenomas (22%) was not found near adenomas. Clinical-pathological correlations are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00612945
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  • 7
    Electronic Resource
    Electronic Resource
    Experimental autoallergic sialadenitis in mice (1983)
    Springer
    Virchows Archiv 400 (1983), S. 143-154 
    Details
    ISSN: 1432-2307
    Keywords: Experimental autoallergic sialadenitis ; Mice ; Submandibular gland ; Histopathology ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental autoallergic sialadenitis was induced in SL/Ni mice by one or two injections of syngeneic submandibular gland homogenate emulsified with adjuvant. Light microscopically, there were marked lymphoid cell infiltration in the submandibular glands with high incidence and proliferation of duct epithelia. Furthermore complete alteration of whole glandular lobules in some cases was observed. Ultrastructurally, small and medium sized lymphocytes and plasma cells constituted a major portion of the infiltrating cells, and lymphocytes were frequently observed inside the basal lamina of ductal and acinar regions, especially observed in the small ductal region. In the aggregates of infiltrating cells, the cell remnants of salivary gland epithelia were scattered. Furthermore some of the epithelial cell remnants in aggregates of infiltrating cells could be recognized as epithelial masses which were composed of proliferated duct epithelial cells, though no typical structure of epimyoepithelial islands seen in Sjögren's syndrome was found. Anti-salivary duct antibody was detected in only one case.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585496
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  • 8
    Electronic Resource
    Electronic Resource
    Undifferentiated carcinoma of the parotid gland (1983)
    Springer
    Virchows Archiv 401 (1983), S. 89-97 
    Details
    ISSN: 1432-2307
    Keywords: Undifferentiated carcinoma ; Parotid gland ; Myoepithelial cell ; Epithelial cell ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two cases of undifferentiated carcinoma of the parotid gland were studied by light and electron microscopy. Light microscopy showed nests of ovoid cells with scanty cytoplasm and pyknotic nuclei in two cases. One case was the small-cell type, and another one was the large-cell type histopathologically. Electron microscopy showed two distinct cell types in each tumor: Case 1 (small-cell type). — An epithelial-like cell, and an irregular-shaped cell containing bundles of filaments suggesting myoepithelial differentiation. Case 2 (large-cell type). — An epithelial-like cell, and a large cell containing secretory-like granules. These findings support a salivary duct epithelial origin for these tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00644792
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  • 9
    Electronic Resource
    Electronic Resource
    Uterine glands of the pig during pregnancy (1983)
    Springer
    Anatomy and embryology 166 (1983), S. 121-134 
    Details
    ISSN: 1432-0568
    Keywords: Uterine glands ; Pig ; Ultrastructure ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the porcine uterine glands is described from material taken from 11 pregnant pigs at exactly known stages of gestation (day 30; 58; 80; 100; 110). Fixation was performed by perfusion via a branch of the uterine artery and the tissue was routinely processed for electrom microscopy. Additionally, cytochemical studies (phosphotungstic acid reaction for glycoproteins, according to Rambourg 1967; acid phosphatase reaction; ultrastructural localization of cellular iron, according to Parmley et al. 1978) were performed. On day 30 of pregnancy the uterine glands are coiled, simple tubular glands with a narrow lumen. The epithelial lining is simple columnar and consists basically of two cell types, ciliated cells and secretory cells. The secretory activity of the glandular epithelium is low; only a few secretory granules are present in the supranuclear cytoplasm. At midpregnancy the ultrastructure of the glands has significantly changed and the cells now show all the characteristics of high secretory activity: numerous parallel cisternae of rough endoplasmic reticulum, an extensively developed Golgi apparatus and many secretory granules which give a positive reaction for acid phosphatase and glycoproteins. The lumina of the glands are significantly enlarged and filled with a great amount of a granular, acid phosphatase-positive material. In the last third of pregnancy, only minor changes in the ultrastructure of the uterine glands are observed. The secretory activity is still high. The amount of rough endoplasmic reticulum has further increased and parallel arrays of cisternae occupy a considerable part of the supranuclear cytoplasm. The importance of the uterine secretion for embryonic nutrition and development is only partly understood. One of the secreted glycoproteins, uteroferrin, is believed to play an important role in the iron transfer from mother to fetus. From midpregnancy onward, a special cell type, the “granule laden cell” is found scattered between normal secretory cells of the uterine glands. Contrary to the opinion of Perry and Cromby (1982), we could demonstrate that these cells frequently extend to the lumen of the gland; hence the term “basal cell” seems inappropriate for this cell type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317948
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural study of the mouse antimesometrial decidua (1983)
    Springer
    Anatomy and embryology 166 (1983), S. 263-274 
    Details
    ISSN: 1432-0568
    Keywords: Decidua ; Ultrastructure ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of mouse antimesometrial decidual cells was analyzed during the development of the decidua between days 5 and 8 of pregnancy. The first decidual cells, appearing on the 5th day, are polygonal with rounded nuclei and prominent nucleoli; free ribosomes predominate in the cytoplasm. On the 6th to the 8th days the cytoplasm of these cells is typically that of cells actively engaged in macromolecular synthesis. Large numbers of granular and agranular endoplasmic reticulum cisternae are present in addition to well-developed Golgi complexes, mitochondria and lysosomes. Many bundles of microfilaments and lipid droplets occur during this period. An intense accumulation of autophagosomes and lysosomes with very heterogeneous content was noted on the 7th and especially the 8th days. The presence of these organelles is an indication that involution of this part of the decidua has begun.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305087
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  • 11
    Electronic Resource
    Electronic Resource
    Effect of prepuberal castration on porcine bulbospongiosus muscle (1983)
    Springer
    Anatomy and embryology 168 (1983), S. 51-58 
    Details
    ISSN: 1432-0568
    Keywords: Bulbospongiosus muscle ; Histochemistry ; Ultrastructure ; Castration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The histochemical profile and ultrastructural properties of the bulbospongiosus muscle (BSM) fibers from 5–6 month old boars and barrows (castrated at 7 days of age), and intact week old piglets were compared. Based on myosin ATPase, preincubated at pH 4.2, BSM of boars contained predominately intermediately staining fibers, whereas BSM of barrows and piglets had a mixture of staining intensities. Fibers from boar BSM stained intensely for SDH, with subsarcolemmal and diffuse location of reaction product. Staining intensity for SDH was variable in BSM from barrows and piglets, with diffuse location of reaction product. The BSM of boars and barrows contained predominately dark fibers when stained for glycogen and phosphorylase, and the fibers were low in stored lipids. While the fibers were smaller in barrow as compared to boar BSM, ultrastructural differences between boar and barrow BSM were not detectable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305398
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  • 12
    Electronic Resource
    Electronic Resource
    Studies of the guinea pig epididymis (1983)
    Springer
    Anatomy and embryology 168 (1983), S. 195-209 
    Details
    ISSN: 1432-0568
    Keywords: Epididymis ; Guinea pig ; Principal cells ; Zonula occludens ; Zonula adhaerens ; Ultrastructure ; Freeze fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The junctional complexes of the principal cells in the guinea pig epididymis were analyzed using freeze fracture and ultrathin section goniometric techniques. Replicas of the seven regions (I to VII) investigated reveal a continuous decrease in the number of tight junctional strands, ranging from 15.73±3.54 in zone I (proximal) to 4.39±0.78 in zone VII (distal tubule). The distance from the adluminal to the basolateral strand also diminishes from proximal, 0.73±0.02 μm to distal, 0.19±0.03 μm. The junctional strands appear on the P-face and anastomose forming compartments which are larger in the basolateral areas than those in the apical. The network of strands frequently form terminal loops and blind endings towards the more basal parts of the lateral membrane. Freeze fracture images also exhibit randomly distributed particulate aggregations which correspond to maculae adhaerentes, the highest number of which are found in zone IV, V, and VII. Desmosomal figures are found not only below, but also adjacent and intermingled among the tight junctional strands. This special junctional arrangement is confirmed upon goniometric analysis of ultrathin sections from zones IV, V, and VII. Electron dense desmosomal plaques are seen parallel and directly subjacent to the membranes of the tight junctions, following the strands in both directions to finally converge on the punctiform connections. Goniometry also reveals a dense feltwork of material closely applied along the lateral cell border. These zonulae adhaerentes are seen to be of greatest length and density in zones I, VI, and VII.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315816
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  • 13
    Electronic Resource
    Electronic Resource
    An ultrastructural study of lactation in the human breast (1983)
    Springer
    Anatomy and embryology 168 (1983), S. 349-359 
    Details
    ISSN: 1432-0568
    Keywords: Breast ; Human ; Lactation ; Ultrastructure ; Morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In this study the morphological features of lactation in the human breast were examined by scanning and transmission electron microscopy. The lactating lobules comprised large numbers of interconnecting acini which were lined by a single layer of epithelial cells with underlying myoepithelial cells. Marked variations were noted in the shape of the epithelial cells. The myoepithelial cells formed an open meshwork of interconnecting cytoplasmic processes packed with myofibrils. The basal cytoplasm of the epithelial cells was packed with rough endoplasmic reticulum while the apical cytoplasm contained a hypertrophic Golgi body, numerous vacuoles (a few of which contained casein micelles), a number of lipid droplets and small coated and uncoated vesicles. The lipid droplets were released by progressive protrusion from the apical surface. They remained covered by the plasmalemma and were finally budded off into the lumen. In certain cases a portion of cytoplasm was released with the lipid droplet. The vacuoles and small vesicles fused with the plasmalemma and released their contents by exocytosis. Within the samples the majority of epithelial cells were actively lactating although examples of undifferentiated “resting” and dead (lysed) cells were also identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304273
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  • 14
    Electronic Resource
    Electronic Resource
    Studies of the guinea pig epididymis (1983)
    Springer
    Anatomy and embryology 168 (1983), S. 173-194 
    Details
    ISSN: 1432-0568
    Keywords: Epididymis ; Guinea pig ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The guinea pig epididymis is subdivided into seven zones. The ultrastructure and morphology of the principal cells in these zones is analyzed. The position, shape and content of the nuclei are variable along the length of the epididymal duct. Features characteristic of absorptive activity, such as micropinocytotic caveolae, vacuoles, and multivesicular bodies are of high concentration in zone IV and VI. The Golgi apparatus, rough endoplasmic reticulum and secretion granules are organelles and inclusions implicated to secretory functions and in this study are not found in the following concurring amounts within the principal cells of the seven zones: the Golgi apparatus exhibits a trend of increase from zone II to zone VII while the rough endoplasmic reticulum decreases. Secretion granules, though, are detected only in zones II and III, not only in the supra-, but also in the peri-and infranuclear regions. This possibly implies an exocrine secretory functions. Lamellar whorls and profiles of tubular smooth endoplasmic reticulum are concentrated in the supranuclear and adluminal regions of zones I, II and VI. A high concentration of large lipid droplets is a consistent feature of the perinuclear region of zone II. Mitochondia and lysosomes are detected in relatively large amounts along the epididymal duct. The correlations of these morphological characteristics with respect to their possible functional role are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315815
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  • 15
    Electronic Resource
    Electronic Resource
    Ultrastructure of the absorptive cells in the small intestine of the rat during starvation (1983)
    Springer
    Anatomy and embryology 168 (1983), S. 331-339 
    Details
    ISSN: 1432-0568
    Keywords: Ultrastructure ; Starvation ; Absorptive cells ; Small intestine ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the absorptive cells in the duodenum, jejunum and ileum after 7, 14 and 21 days of starvation was investigated using rats aged from 12 to 18 months weighing about 500 g. In the basal cytoplasm of the absorptive cells (in the duodenum and ileum of 21-day-starved rats and the jejunum of 14- and 21-day-starved rats), the following changes were found: atrophied mitochondrion-like bodies, small vesicles, a short and sparse rough-surfaced endoplasmic reticulum and a lack of density in a portion of the cytoplasm. Moreover, many autolysosomes of various sizes and shapes were encountered in the basal cytoplasm; occasionally these elements accumulated and appeared to fuse to one another. In contrast, in the apical cytoplasm of absorptive cells in the intestine of starved rats, the ultrastructure was similar to that of control rats. It was considered that the apical cytoplasm of the absorptive cells in the starved rat intestine might be preserved as long as possible during starvation in order to absord nutrients when they become available again.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304271
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  • 16
    Electronic Resource
    Electronic Resource
    A fine structural study of the cells that proliferate in the partially denervated dentate gyrus of the rat (1983)
    Springer
    Anatomy and embryology 166 (1983), S. 317-332 
    Details
    ISSN: 1432-0568
    Keywords: Dentate gyrus ; 3H-thymidine ; Glial cells ; Proliferation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tritiated thymidine autoradiography has established that after interrupting the commissural afferents to the dentate gyrus a number of non-neuronal cells proliferate in the molecular layer. In the present study the fine structure of the proliferating cells was analyzed by reembedding the 2-μm thick plastic sections of the dentate gyrus which had been previously coated with a nuclear emulsion and processed for light microscopic autoradiography. The location of the labeled cells was plotted with a camera lucida and a few ultrathin sections were taken from the re-embedded sections. In these the labeled cells were re-identified and photographed in an electron microscope. Most of the identified proliferating cells exhibited the following morphological features: The nuclei were irregularly oval, sometimes with deep indentations and contained dense clumps of chromatin; their diameters ranged between 4.5 and 6.5 μm. The cytoplasm was generally disposed to one side of the nucleus and often extended into a few broad processes. The Golgi apparatus was well developed. Many rosettes of free ribosomes were scattered throughout the cytoplasm, and the rough endoplasmic reticulum usually consisted of a few short cisternae. Small multilamellated bodies were common, but dense inclusion bodies were infrequent. The observations reported in this paper suggest: 1. that the nonneuronal cells which proliferate in a neuropil undergoing a mild denervation are morphologically closely related to microglia; 2. that in young adult animals these cells do not seem to have been previously involved in intense phagocytic activity; and 3. that the proliferating cells are present in the neuropil at the time of the denervation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305921
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  • 17
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of adenylate cyclase and cAMP phosphodiesterase in the gastrulating chick embryo (1983)
    Springer
    Development genes and evolution 192 (1983), S. 42-44 
    Details
    ISSN: 1432-041X
    Keywords: Chick embryo ; Gastrulation ; Adenylate cyclase ; cAMP phosphodiesterase ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructural localization of adenylate cyclase (E.C. 4.6.1.1.) and cAMP phosphodiesterase (PDE) (E.C. 3.1.4.17.) in the ectoderm of the developmental stage 4 chick embryo was studied. Adenylate cyclase was localized in the lateral surfaces of the ectodermal cells. In the primitive streak cells the enzymatic activity was observed on all the lateral surfaces, whereas in the periphery of the blastoderm the reaction product was localized in the apical parts of the lateral plasma membranes only. cAMP PDE localized in the apical cytoplasm of the ectodermal cells, with highest activity in the globular projections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848768
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  • 18
    Electronic Resource
    Electronic Resource
    An ultrastructural study of experimental high velocity penetrating head injury (1983)
    Springer
    Acta neuropathologica 59 (1983), S. 277-282 
    Details
    ISSN: 1432-0533
    Keywords: Trauma ; Missile head injury ; Astrocyte ; Blood brain barrier ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental high velocity missile brain injury in the rhesus monkey produces widespread swelling of perivascular astrocytes within 30 min of injury. Possible mechanisms for this lesion include a direct effect of force, chemical mediation secondary to the extravasation of blood, alterations in the permeability of the blood brain barrier and ischaemia. The implications of this finding for the function of the blood brain barrier, for neurotransmission and for neuronal survival are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691493
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  • 19
    Electronic Resource
    Electronic Resource
    Oligodendroglioma-like cells (clear cells) in ependymoma (1983)
    Springer
    Acta neuropathologica 62 (1983), S. 141-144 
    Details
    ISSN: 1432-0533
    Keywords: Ependymoma ; Clear cells ; Oligodendroglioma-like cells ; Mixed glioma ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A brain tumor of a 22-year-old man was composed mostly of round cells with perinuclear halos (clear cells), forming clusters intersected by small blood vessels. In some areas, the tumor cells showed perivascular arrangement and epithelial pattern. Phosphotungstic-acid hematoxylin stain and immunoper-oxidase stain for glial fibrillary acidic protein (GFAP) technique failed to stain the clear cells. Electron microscopy of the clear cells revealed them to be classical ependymoma cells with well developed intercellular junctions, microvilli and cilia. As no reporters in the past showed the evidence to clarify the nature of the clear cells, this case is considered a good example to support the viewpoint that the clear cells (oligodendroglioma-like cells) commonly observed in ependymomas are in reality ependymoma cells. It is stressed that the diagnosis of “mixed glioma” or “oligoependymoma” should be made with sufficient caution despite the recent advances of GFAP technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684931
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  • 20
    Electronic Resource
    Electronic Resource
    An ultrastructural study of epidermolytic leukoplakia (1983)
    Springer
    Archives of dermatological research 275 (1983), S. 86-91 
    Details
    ISSN: 1432-069X
    Keywords: Vermilion of the lip ; Leukoplakia ; Epidermolytic hyperkeratosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A leukoplakic lesion of the lip showing the histologic features of epidermolytic hyperkeratosis above solar elastosis was investigated by electron microscopy. The ultrastructural alterations observed in the upper epidermal layers corresponded in the main with those of epidermolytic hyperkeratosis in other skin diseases. The keratinocytes showed irregularly formed tonofilaments, a marked intracellular edema, and premature cornification. In addition, there were discrete subcellular signs of premalignancy in the cells of the basal and suprabasal layers. These alterations suggest that the epidermolytic leukoplakia represents a rare histopathologic variant of actinic cheilitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412880
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  • 21
    Electronic Resource
    Electronic Resource
    Chroococcus S24 and Chroococcus N41 (cyanobacteria): morphological, biochemical and genetic characterization and effects of water stress on ultrastructure (1983)
    Springer
    Archives of microbiology 135 (1983), S. 81-90 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Ultrastructure ; Nitrogen fixation ; Water stress ; Taxonomy ; DNA ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains of desiccation-tolerant coccoid cyanobacteria, Chroococcus S24, a marine form, and Chroococcus N41, a cryptoendolith isolated from a hot-desert rock, have been characterized. The mol % DNA base compositions of the strains are 47.1 and 48.9% respectively. Plasmid DNA was not detected in either strain. The pigment contents and nutritional characteristics of the strains are identical. Both lack phycoerythrinoid pigments and, in culture, behave as slow-growing halotolerant marine forms with elevated requirements for Na+, Cl−, Mg2+ and Ca2+. Sucrose was the only carbon source of those tested that supported photoheterotrophic growth. Each strain synthesizes nitrogenase under anaerobic conditions but not in air. Morphologically the two strains are indistinguishable. They are considered to be independent isolates of the same cyanobacterial species. Chroococcus N41 was studied in detail with the electron microscope. When brought to equilibrium at matric water potentials of-168 MPa and lower (to-673 MPa=c0.12a w) the protoplast shrinks, but the cells maintain the same size and diameter as those at-2,156 kPa (MN medium; control); the sheath expands and remains attached to the cell wall outer membrane by fibrils. The cell wall, cell membrane, thylakoid membranes, cyanophycin granules and carboxysomes appeared intact in desiccated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408014
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  • 22
    Electronic Resource
    Electronic Resource
    Ultrastructural demonstration of a projection from the flocculus to the nucleus prepositus hypoglossi in the cat (1983)
    Springer
    Experimental brain research 51 (1983), S. 192-198 
    Details
    ISSN: 1432-1106
    Keywords: Flocculus ; Nucleus prepositus hypoglossi ; Ultrastructure ; Degeneration ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Small electrolytic lesions were made in the flocculus of two adult cats by means of a stereotactic approach avoiding any damage to the cerebellar nuclei. After a survival time of 3 days the animals were killed and the brains fixed and prepared according to standard procedures for ultrastructural studies. The brains of two unoperated cats were similarly treated and served as normal controls. In the experimental animals a large number of boutons in the rostral part of the nucleus prepositus hypoglossi (Ph) ipsilateral to the floccular lesion showed degenerative changes. These were characterized by hypertrophy, a prominent aggregation of densely packed parallel tubules or concentric arrays of cisternae and a filamentous hyperplasia. Only very rarely were such abnormal boutons seen in the caudal half of the ipsilateral Ph, or on the contralateral side or in the unoperated animals. The degenerating boutons contain clusters of pleomorphic vesicles and they establish symmetrical synaptic contacts with somata, dendritic shafts and dendritic spines. Some of the degenerating boutons appear to be of the en passant type. These findings thus affirm the existence of a direct flocculo-prepositus projection in the cat. It is suggested that this pathway could be responsible for mediating information about eye position and velocity to Ph neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237194
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  • 23
    Electronic Resource
    Electronic Resource
    Ultrastructure of tuberculate spores in Streptomyces thermoviolaceus (1983)
    Springer
    Archives of microbiology 134 (1983), S. 295-298 
    Details
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Streptomyces thermoviolaceus ; Sporogenesis ; Spore ornamentation ; Cupular knobs ; Sheath ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sporogenesis of aerial spores in Streptomyces thermoviolaceus corresponded to a common sporulation type in the genus. The sporulation septum was composed of an outer ring-shaped constriction wall and an inner interspace septum arising by the inwards growth of a double annulus. In mature spores the wall was composed of two layers, the outer one was part of the parent hyphal wall and septum material, the inner one was formed de novo. The spore chains were enclosed by the thin breakable sheath containing small rod-like elements. The ornamentation in the form of knobs, which were a characteristic feature of the species originated from the sheath. The knobs were hemispherical particles with an inner electron dense core and an outer electron transparent shell. The term “cupular knobs” was suggested for this type of tuberculate ornamentation. Frequently, the knobs became detached from the surface in which case the inner core separated easily from the shell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407805
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  • 24
    Electronic Resource
    Electronic Resource
    A sertoli-leydig cell tumor and pregnancy (1983)
    Springer
    Archives of gynecology and obstetrics 233 (1983), S. 295-308 
    Details
    ISSN: 1432-0711
    Keywords: Sertoli-Leydig cell tumor ; Pregnancy ; Catheterization ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An extremely rare case of a conception occurring in a 26-year-old patient with a small virilizing Sertoli-Leydig cell tumor (diameter: 0.6 cm), bilateral polycystic ovaries and non-tumorous adrenal hyperandrogenism is presented. Prepregnancy findings included hirsutism, clitoromegaly, secondary amenorrhea, and elevated peripheral plasma testosterone (T; 5.7 ng/ml). Extensive basal steroid screening, dynamic function tests, conventional radiologic procedures, selective glandular vein catheterization, and laparoscopy failed to localize unequivocally the source of androgen excess, but suggested bilateral adrenal involvement. The patient conceived during the diagnostic work-up; peripheral T levels increased to 12.1 ng/ml within the first trimester. An exploratory laparotomy with left adrenalectomy, right adrenal biopsy and left ovarian wedge resection revealed an incompletely removed Sertoli-Leydig cell tumor, but normal adrenal histology. The pregnancy was terminated, a left oophorectomy and right ovarian wedge resection were performed at 14 weeks' gestation. Subsequently, peripheral androgens returned to normal, regular menses resumed, and hirsutism disappeared. Three years later the patient delivered a healthy female infant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133804
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  • 25
    Electronic Resource
    Electronic Resource
    Electron microscopical study of self-differentiation potency in the chick embryonic endoderm cultured in vitro (1983)
    Springer
    Development genes and evolution 192 (1983), S. 171-178 
    Details
    ISSN: 1432-041X
    Keywords: Differentiation ; Digestive tract ; Endoderm ; Organ culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The self-differentiation potency of the endoderm of the chick embryo was investigated mainly by transmission electron microscopy. Endodermal fragments isolated from 4- to 6-day stomach or small intestine were cultured in the absence of mesenchyme and were able to differentiate in vitro into organ-specific epithelia. Endodermal fragments isolated from the stomach region differentiated into a pseudo-stratified epithelium with periodic acid Schiff-positive mucous granules in the apical cytoplasm, while those from the small intestinal region differentiated into a simple columnar epithelium with a striated border which was positive in alkaline phosphatase activity. These features are comparable with those of the mucous secretory epithelium of the normal embryonic stomach and the absorptive epithelium of normal embryonic small intestine, respectively. Next, the self-differentiation potencies were investigated of the upper and lower layers of the blastoderms, at stages 1–5 of Hamburger and Hamilton (H. and H.). Both stomach-type and small-intestine-type epithelia developed only when fragments of the lower layer isolated from the blastoderms older than stage 3 of H. and H. were cultured, suggesting that cells possessing the potency to differentiate into the stomach- and small-intestine-type epithelia exist in the definitive endoderm at the beginning of its formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848687
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  • 26
    Electronic Resource
    Electronic Resource
    Immunoagglutination of type-C virus particles in a human T-cell line by serum supplementation from patients with adult T-cell leukemia (1983)
    Springer
    Journal of cancer research and clinical oncology 105 (1983), S. 106-108 
    Details
    ISSN: 1432-1335
    Keywords: Human T-cell line ; Type-C virus particles ; Adult T-cell leukemia ; Immunoagglutination ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A T-cell line, MT-2, derived from human cord blood lymphocytes by cocultivation with adult T-cell leukemia (ATL) cells is a continuous producer of type-C virus particles. Electron microscopy of MT-2 cells cultured for 1–3 weeks in medium containing 10% ATL patients' sera revealed agglutination of type-C virus particles within the electron-dense deposits in the extracellular spaces. No such agglutination occurred in control cultures supplemented with normal human or fetal calf serum. These results provide direct evidence for the specific reactivity of ATL patients' sera with type-C virus particles in the MT-2 cell line at the ultrastructural level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391841
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  • 27
    Electronic Resource
    Electronic Resource
    Ultrastructural study on nervous system of fetus with GM1-gangliosidosis type 1 (1983)
    Springer
    Acta neuropathologica 61 (1983), S. 15-20 
    Details
    ISSN: 1432-0533
    Keywords: Ultrastructure ; Fetus ; Nervous system ; GM1-gangliosidosis type 1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The nervous system of a 22-week-old fetus with GM1-gangliosidosis type 1 was studied by electron microscopy. The tissues thus examined were the cerebral cortex at the parietal region, the cerebellum, the thoracic spinal cord, the Auerbach's myenteric plexus in the large intestine and the radial nerve fibers. In the cerebral cortex, membrane-bound vacuoles, which occasionally contained stacks of fine fibrils, were observed in the large young neurons in the deeper part of the cortical plate. The neurons in the other part of the cerebral cortex carried no storage materials. In the cerebellum, the membrane-bound vacuoles with stacks of fine fibrils were seen only in the Purkinje cells. The neurons in the spinal cord also contained several zebra-like bodies and the above membrane-bound vacuoles. As for the peripheral nervous system (PNS), neurons in the Auerbach's myenteric plexus carried membranous cytoplasmic bodies and zebra-like bodies. Some of the axons in the radial nerve fibers also contained a lot of pleomorphic electron-dense bodies and a few membranous cytoplasmic ones. These results show that the accumulation of storage materials is started in the large neurons which are produced in the early stage of neurogenesis in the central nervous system (CNS). Additionally, the observed membrane-bound vacuoles are considered to be structures which occur before the membranous cytoplasmic bodies and/or the zebra-like bodies. It is also elucidated that the PNS is affected earlier than the cerebral and cerebellar cortices and thoracic spinal cord.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688381
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  • 28
    Electronic Resource
    Electronic Resource
    Cerebral ischemia in the rat: Ultrastructural and morphometric analysis of synapses in stratum radiatum of the hippocampal CA-1 region (1983)
    Springer
    Acta neuropathologica 61 (1983), S. 52-60 
    Details
    ISSN: 1432-0533
    Keywords: Ischemia ; Brain ; Hippocampus ; Synapses ; Ultrastructure ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 10-min long ischemic insult followed by up to 60-min survival results in several changes of the synaptic ultrastructure in the hippocampal CA-1 region. The alternations consist of gradual change of synaptic curvature from neutral to positive, cleavage and decrease in thickness of the postsynaptic densities and, in the case of many terminals, wrinkling of their profiles. The most striking form of damage are membrane discontinuities which begin to appear in very small numbers after 20 min of blood reflow and become much more pronounced after 60 min. The development of those modifications seems to be time-related, whereas decrease in the number of synaptic vesicles, as shown by the morphometric analysis, occurs after 10 min and does not progress any further after 20 and 60 min. This decrease is most pronounced in the immediate vicinity of the presynaptic membrane. Although the observed signs of ultrastructural alternations of synapses in the postischemic period appear to conform to the general pattern of synaptic degeneration observed under other conditions, the severity of ischemia is underlined with the rate at which those changes develop, thus pointing toward grossly disturbed metabolism of postischemic neurons. Recently, a number of theories have been advanced, discussing significance of ischemic destruction of membrane phospholipids. These theories are discussed in the context of membrane discontinuities reported in this investigation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688386
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  • 29
    Electronic Resource
    Electronic Resource
    Neurofibromatosis tumor and skin cells in culture (1983)
    Springer
    Acta neuropathologica 61 (1983), S. 275-282 
    Details
    ISSN: 1432-0533
    Keywords: Neurofibromatosis ; Cell culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Skin fibroblasts and tumor cells were cultured from four patients with peripheral von Recklinghausen's neurofibromatosis (NF). The cell type enriched in culture from the tumors carried the fibroblastic Thy 1.1. cell surface antigen and produced fibronectin, like fibroblasts from skin of NF-patients or from control persons. In electron micrographs the NF tumor and NF skin cells were similar to the control skin fibroblasts; elongated in shape, contained tubular mitochondria, variable amounts of granular endoplasmic reticulum, numerous lysosomal inclusion bodies and collections of 5 nm filaments. Trypsinized cells were fractionated with centrifugation in a Percoll density gradient. All cell lines produced only one sharp band of viable cells at the buoyant density of 1.03. Compared with the NF skin or control skin fibroblasts the NF tumor cells, however, produced a less well organized peri-and extracellular matrix estimated from fibronectin fluorescence. The nuclear sizes were measured from photographs of the cultures. The nuclei of all four tumor cell lines were larger than those of the skin fibroblasts of the corresponding patients. Neurofibromatosis tumor cells thus resemble skin fibroblasts in their density and in some ultrastructural properties but are different in their growth pattern and synthetic functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691998
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  • 30
    Electronic Resource
    Electronic Resource
    Neuropathological findings of an autopsy case of adult β-galactosidase and neuraminidase deficiency (1983)
    Springer
    Acta neuropathologica 61 (1983), S. 283-290 
    Details
    ISSN: 1432-0533
    Keywords: β-Galactosidase and neuraminidase deficiency ; Neuronal inclusion bodies ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An autopsy case of a Japanese male with familial β-galactosidase and neuraminidase deficiency is reported. The clinical picture was characterized by adult onset, a gargoyle-like face, cerebellar ataxia, myoclonus, convulsions, retinal degeneration and cortical blindness. Histopathologically, most neurons seemed to have become degenerated in the whole cerebral cortex. Moreover, the calcarine cortex appeared spongy with depopulation of nerve cells. Stuffed neurons or neuronal storage changes were found throughout the brain, especially in the motor nuclei of the spinal cord and brain stem. The inclusions in the stuffed neurons revealed various profiles on the electron microscope. They were composed of membranous lamellar and/or multilamellar structures, often accompanying vacuoles and reminiscent of lipofuscin-like profiles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691999
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  • 31
    Electronic Resource
    Electronic Resource
    Pituitary adenomas: Immunohistology and ultrastructural analysis of 118 tumors (1983)
    Springer
    Acta neuropathologica 62 (1983), S. 1-14 
    Details
    ISSN: 1432-0533
    Keywords: Pituitary adenomas ; Immunohistology ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An analysis is presented of the immunohistological and ultrastructural features in a series of 118 surgically removed pituitary adenomas all of which were studied immunohistologically using antisera to growth hormone (GH), prolactin (PRL) ACTH, βFSH, βLH and βTSH, and 75 of which were studied ultrastructurally. Results were analysed according to the mode of presentation of patients. Forty-one (35%) of the tumours were from patients with acromegaly or gigantism, ten (9%) from patients with Cushing's syndrome or Nelson's syndrome, 19 (16%) from patients with clinical features associated with hyperprolactinaemia and 48 (40%) from patients with space occupying lesions which appeared clinically to be overtly endocrinologically functionless. By light microscopy, using the immunoperoxidase (PAP) technique, immunoreactive GH was demonstrated in all the tumours from patients with acromegaly or gigantism, immunoreactive ACTH in all tumours from patients with Cushing's syndrome or Nelson's syndrome and immunoreactive PRL in 95% of tumours associated with effects of hyperprolactinaemia. Forty-five percent of the tumours from acromegalic patients contained some PRL-positive cells as well as GH-positive cells. Among the tumours which appeared clinically to be endocrinologically functionless were three tumours (from males) uniformly stained for immunoreactive PRL. Of the remainder, 60% were negative for immunoreactive hormones and 40% contained small numbers of cells which were positive for a variety of immunoreactive hormones. ACTH-cell and PRL-cell tumours had ultrastructural features as described in previous studies. Fifty percent of GH-cell tumours examined at the EM level contained fibrous bodies, while in the remainder these structures were not identified. Tumours with fibrous bodies were more likely to contain PRL as well as GH with immunoperoxidase. All tumours that were endocrinologically functionless and which were examined at the EM level contained secretory granules. Oncocytic change was common in these tumours. No ultrastructural differences were observed between those which contained immunoreactive hormones by light microscopy and those which did not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684914
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  • 32
    Electronic Resource
    Electronic Resource
    Specific ultrastructural markers of human pinealomas (1983)
    Springer
    Acta neuropathologica 62 (1983), S. 31-40 
    Details
    ISSN: 1432-0533
    Keywords: Human pinealomas ; Ultrastructure ; Specific markers ; Pinealocyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An ultrastructural study of four pinealomas was carried out to precise eventual specific markers. Dark and clear cells joined with zonulae adherents, extensive and pleiomorphous processes, a complex vacuolar system, and characteristic organelles (lysosome-like structures, clear and dense-core vesicles, vesicle-crowned rodlets and related structures, microtubular sheaves and centriolar derivatives, membranous whorls, fibrous bodies, microtubules, heterogeneous cytoplasmic inclusions) offered a typical pattern. No correlation could be made between the histological and ultrastructural features. The authors stress the ultrastructural similarities between the human tumor cells and the mammalian pineal cells. Pinealomas appeared as a morphological entity distinct from neuronal and astrocytic tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684917
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  • 33
    Electronic Resource
    Electronic Resource
    Some aspects of the development of the endodermis and cortex ofTilia cordata andPicea sitchensis (1983)
    Springer
    Plant and soil 71 (1983), S. 147-153 
    Details
    ISSN: 1573-5036
    Keywords: Cortex ; Endodermis ; Picea sitchensis ; Tilia cordata ; Transfer cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The endodermis of bothTilia cordata andPicae citchensis progressess through 3 characteristic phases of development. These developments are delayed somewhat in the xylem pole endodermis ofT. cordata, while inP. sitchensis 3–5 passage cells are found. The cortex ofT. cordata is characterised by very thick walls, while that ofP. sitchensis is characterised by a thick walled layer just outside the endodermis and by 2–3 outer layers of transfer cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182649
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  • 34
    Electronic Resource
    Electronic Resource
    Effect of hyperthermic vesical irrigation with bleomycin on the ultrastructure of the well-differentiated tumour and non-tumorous mucosa of the human urinary bladder (1983)
    Springer
    Urological research 11 (1983), S. 131-137 
    Details
    ISSN: 1434-0879
    Keywords: Human bladder tumour ; Non-tumorous human bladder mucosa ; Hyperthermic vesical irrigation ; Bleomycin ; Ultrastructure ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Bladder tumours and non-tumorous bladder mucosa were studied by scanning and transmission electron microscopy in seven patients who had undergone hyperthermic vesical irrigation with bleomycin. The treatment induced sloughing of the outermost tumour cells, an increase of blebs and a decrease of cytoplasmic processes of the deeply located tumour cells as well as cellular degeneration. Although less severe, non-tumorous mucosa showed similar changes. Microvilli also appeared on the superficial cells of non-tumorous mucosa after the treatment. This treatment is effective by inducing cell degeneration and desquamation but not selective to the bladder tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257718
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  • 35
    Electronic Resource
    Electronic Resource
    Changes in the satellite cells of the anuran tadpole tail muscles during metamorphosis (1983)
    Springer
    Cell & tissue research 228 (1983), S. 573-585 
    Details
    ISSN: 1432-0878
    Keywords: Satellite cells ; Muscle fibres ; Anura ; Metamorphosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The incidence and the ultrastructure of the satellite cells associated with red and white muscle fibres of the anuran (Rana japonica) tadpole tail were examined in three stages (hindlimb-bud, prometamorphic, and climax) during metamorphosis. In these three stages the satellite cells are found more frequently in the red fibres than in the white fibres. As metamorphosis proceeds, the incidence of the satellite cells decreases in both red and white fibres. The satellite cells in the hindlimb-bud stage have abundant cytoplasm rich in organells. Cisternae of rough endoplasmic reticulum are often dilated. The intercellular space between the satellite cell and the muscle fibre is narrow and relatively constant (15–25 nm). Some satellite cells appear to be in the process of fusion with the muscle fibre. Cytoplasmic volume and organellar content are gradually reduced during metamorphosis. In the prometamorphic and climax stages, the intercellular space is in general gradually extended. It often exceeds 0.5 μm, and usually contains the basal lamina and fibrous materials. Some satellite cells have cytoplasmic processes that project into the interstitial space through a gap in the basal lamina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211477
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  • 36
    Electronic Resource
    Electronic Resource
    Ultrastructure and differentiation of ascidian muscle (1983)
    Springer
    Cell & tissue research 230 (1983), S. 77-94 
    Details
    ISSN: 1432-0878
    Keywords: Ascidian larva ; Embryology ; Myogenesis ; Somatic striated (skeletal) muscle ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The larval muscle cells of Diplosoma macdonaldi contain subcortical and medullary myofibrils which are invested by fenestrated sheets of the sarcoplasmic reticulum. Cisternae of the sarcoplasmic reticulum are coupled with tubular invaginations of the sarcolemma. To appreciate better such uncommon features of cellular organization, six embryonic stages were selected for an ultrastructural study of myogenesis. The proliferative, synthetic, and elaborative phases of myogenesis were represented by embryos ranging from neurulae to prehatching larvae. The contractile apparatus originates during the synthetic phase of myogenesis, when thick and thin myofilaments appear in the cortical sarcoplasm at the epidermal and notochordal poles of the cell. The myofilaments promptly aggregate into unstriated fascicles, and the fascicles unite in series to establish the rudimentary myofibrils. All major sarcomeric bands, except the Z-lines, are evident along the myofibrils. Cisternae of the sarcoplasmic reticulum form peripheral couplings with the overlying sarcolemma, and they also form interior couplings with sarcolemmal invaginations from the ends of the cell. The interior couplings localize over the I-bands of the myofibrils. In the elaborative phase of myogenesis, mitochondria invade the cortical sarcoplasm, and the contractile apparatus passively shifts to the subcortex and medulla of the cell. Relocation of the myofibrils coincides with the disappearance of all peripheral couplings. Cisternae of the sarcoplasmic reticulum anastomose around the myofibrils, creating the fenestrated sheets that extend between sarcomeres. As Z-lines begin to bisect the I-bands, the perifibrillar cisternae become confluent with the cisternae in the precocious interior couplings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216029
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  • 37
    Electronic Resource
    Electronic Resource
    The demonstration of close nerve-Purkinje fibre contacts in false tendons of sheep heart (1983)
    Springer
    Cell & tissue research 230 (1983), S. 105-111 
    Details
    ISSN: 1432-0878
    Keywords: Heart ; Purkinje fibre ; Innervation ; Ultrastructure ; Scanning electron microscopy ; Transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An observation of intimate nerve-Purkinje fibre associations in false tendons of sheep heart is reported. Nerve bundles were observed in deep clefts of Purkinje fibres, in channels running between coupled Purkinje cells and embedded within Purkinje cells, as well as in the outer connective tissue sheath. Most nerve terminals in these areas were filled with small clear vesicles and a few large dense-cored vesicles. Only a few axons with many small dense-cored vesicles were observed. Intimate associations (separation, 60 to 90 nm) between the Purkinje cell and nerve varicosity were observed in the deep clefts. Similar close appositions were also present where nerves were embedded in Purkinje cells. In these cases the Purkinje cell enclosing the nerve bundle formed intercellular junctions with its own sarcolemma. Elaborate sarcolemmal folds with multi-vesicular bodies were also frequently observed near nerve bundles and varicosities. The identity of the transmitter is unknown although the nerves forming intimate associations with Purkinje cells have a morphology typical of cholinergic nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216031
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  • 38
    Electronic Resource
    Electronic Resource
    Glomerular ultrastructure of the trout, Salmo gairdneri (1983)
    Springer
    Cell & tissue research 230 (1983), S. 205-218 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Glomerulus ; Ultrastructure ; Teleosts ; Seawater adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The epithelial ultrastructure of the glomerulus of fresh- and sea-water adapted rainbow trout, Salmo gairdneri was examined by scanning and transmission electron microscopy after in vivo-perfusion fixation. Adaptation to seawater for a period of up to 9 weeks was not associated with a change in glomerular size, although after one year in seawater both glomeruli and their capsules were significantly smaller than the glomeruli of freshwater adapted fish. In freshwater adapted trout the epithelial ultrastructure conformed to the general vertebrate pattern, with rounded podocytes, well-defined primary processes and interdigitating pedicels. Adaptation to seawater was associated with a marked increase in the frequency of cytoplasmic microprojections. In many glomeruli there was a flattening of the podocytes, broadening of the primary processes, and areas of closely-packed pedicels were common. These observations are discussed in the light of previous studies on the mammalian kidney and the changes of single nephron function known to be associated with adaptation of trout to increased environmental salinities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216040
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  • 39
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    Electronic Resource
    Structure of the pars distalis in pouch-young tammar wallabies (Macropus eugenii) (1983)
    Springer
    Cell & tissue research 230 (1983), S. 587-603 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Tammar wallaby ; Marsupial ; Neonate ; Ultrastructure ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pars distalis of pouch-young wallabies (Macropus eugenii) aged 1 to 50 days was studied by means of light-microscopic immunohistochemistry and electron microscopy. In the pars distalis of these pouch-young presumptive somatotrops, which constituted up to 70% of the gland, and nongranulated cells were the most numerous cell types. Small numbers (together representing less than 30% of the pars distalis) of immunoreactive mammotrops, thyrotrops, gonadotrops and corticotrops were also found. The presumptive mammotrops, gonadotrops and thyrotrops increased in number and apparent activity between 1 and 50 days postpartum. Presumptive corticotrop cells in 25 to 30 day-old animals were relatively most numerous, and apparently more active than at any other stage of pouch life; these cells decreased in apparent activity and relative number in older animals. The changes in number and activity of cell types in the pars distalis correlated well with major developmental events such as the onset of adrenal activity, the rapid growth phase in the first 100 days postpartum, and the generally low thyroid activity in pouch-young of less than 50 days of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216203
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  • 40
    Electronic Resource
    Electronic Resource
    The role of connective tissue in the maintenance of epithelial differentiation in the adult (1983)
    Springer
    Cell & tissue research 230 (1983), S. 615-630 
    Details
    ISSN: 1432-0878
    Keywords: Epidermis ; Mouth mucosa ; Connective tissue ; Cell differentiation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specimens of buccal mucosa and ear skin were introduced into the uteri of isologous rats and the animals maintained under estrogen stimulation for ten weeks, whereupon the uteri were removed and processed for light- and electron-microscopic examination. The majority of implants were successful with the epithelium having migrated to replace the adjacent uterine epithelium. Epidermis and oral epithelium growing on its own connective tissue in the uterus showed a normal pattern of histodifferentiation, including the formation of a thickened keratinized surface and appendages such as hair and sebaceous glands. Ultrastructurally, maturation was similar to that of normal tissue. Epidermis growing on uterine connective tissue did not form appendages but the ultrastructural pattern of cytodifferentiation was similar to control tissue. Buccal epithelium on the uterine stroma was markedly altered in its pattern of maturation, the epithelium appearing thinner than usual and showing a pattern of maturation resembling that of non-keratinization. These results suggest that normal histodifferentiation in epidermis and oral epithelium requires the presence of the appropriate connective tissue; in its absence epidermis has an intrinsic capacity for more or less normal cytodifferentiation but this is lacking in the buccal epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216205
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  • 41
    Electronic Resource
    Electronic Resource
    Ultrastructure and morphometric analysis of preimplantation mouse embryos (1983)
    Springer
    Cell & tissue research 230 (1983), S. 661-670 
    Details
    ISSN: 1432-0878
    Keywords: Preimplantation embryos ; Mouse ; Ultrastructure ; Morphometric analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary During cleavage, both qualitative and quantitative morphologic characters of mouse ova change. Up to the 8-cell stage, the volume density of mitochondria remains nearly the same although it increases during early and late blastocyst stages. While a rise of the volume fraction of granular endoplasmic reticulum is noticed during cleavage, the volume density of agranular endoplasmic reticulum diminishes gradually from the 1-cell stage onwards. An increase in the volume fraction of autophagic vacuoles is found, the maximum being reached in early blastocysts. The volume fraction of crystalloid inclusions slightly increases after the 4-cell stage, but this increase is statistically insignificant. The volume density of filamentous material (plaques) conspicuously decreases in all studied embryos from the beginning of cleavage. Starting with the 2-cell stage, the volume fraction of lipid droplets remains practically unchanged. No differences in volume densities of the Golgi apparatus, multivesicular and residual bodies, and large cytoplasmic vesicles with medium electron-dense content are found between the respective cleavage stages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216209
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  • 42
    Electronic Resource
    Electronic Resource
    Ultrastructural studies on sporogony of Babesia microti in salivary gland cells of the tick Ixodes dammini (1983)
    Springer
    Cell & tissue research 231 (1983), S. 275-287 
    Details
    ISSN: 1432-0878
    Keywords: Babesia ; Tick ; Sporogony ; Salivary gland ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tick larvae were permitted to feed on infected hamsters and then allowed to molt. Nymphs were examined just prior to feeding on uninfected hamsters or at timed intervals thereafter. Invasion of the salivary gland by B. microti occurs before feeding of the nymph begins, and development of the parasite is further stimulated by feeding. The sporoblast forms a massive multinucleated meshwork which ramifies throughout the large host cell. No separation of the meshwork into multiple subdivisions, termed “cytomeres” by other workers, has been detected. Instead the specialized organelles characteristic of sporozoites, namely micronemes, rhoptries, and segments of double membrane appear in the meshwork itself and gradually become organized into sporozoite anlagen which protrude from its surface. At the same time the meshwork shortens and thickens giving rise to large compact undifferentiated bodies whose surface is also studded with sporozoite anlagen. Sporozoites thus originate either from the meshwork or from the undifferentiated bodies. In either case large lobate nuclei send projections into the anlagen as they protrude from the surface of the sporoblast. In a final step the mature sporozoites arise by simultaneous nuclear and cytoplasmic divisions. There is no separate stage of schizogony and the process is one of true budding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222180
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  • 43
    Electronic Resource
    Electronic Resource
    Structural and functional studies of the adrenal zona glomerulosa in sodium-depleted and sodium-loaded sheep (1983)
    Springer
    Cell & tissue research 229 (1983), S. 515-531 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal zona glomerulosa ; Ultrastructure ; Sodium depletion ; Sodium loading ; Sheep
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of alterations in sodium status upon the morphology of the adrenal zona glomerulosa in sheep have been examined qualitatively and quantitatively, using lightand electron microscopy, and correlated with functionally related biochemical data. With severe sodium depletion induced by parotid-cannula drainage, there was mitotic activity throughout the zona glomerulosa, and glandular cells showed striking ultrastructural changes. These changes particularly affected mitochondria, which were enlarged, rounded and showed replacement of their normal lamelliform cristae by thin elongated cristal elements and bundles of tubular “rod-like” structures. Quantitative morphometric studies showed an increase in the volumes of zona glomerulosa cells, nuclei, mitochondria, smooth and granular endoplasmic reticulum, and Golgi profiles. In contrast, with dietary sodium loading, zona glomerulosa cells appeared shrunken and showed cytoplasmic lipid accumulation; mitochondria and other organelles were not significantly altered. The correlation of the ultrastructural cytological alterations in zona glomerulosa cells in sodium-depleted sheep with raised blood aldosterone levels suggests that such morphologic changes reflect a heightened capacity of these cells for aldosterone biosynthesis and secretion. These changes may also account for the increased sensitivity of the zona glomerulosa to aldosterone-producing stimuli during sodium deficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00207695
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  • 44
    Electronic Resource
    Electronic Resource
    Putative sense organs on the palliai tentacles of the limpet, Patella vulgata (L.) (1983)
    Springer
    Cell & tissue research 231 (1983), S. 663-674 
    Details
    ISSN: 1432-0878
    Keywords: Ciliary tuft ; Pallil tentacles ; Patella vulgata ; Sensory ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure and ultrastructure of ciliary tufts on the pallil tentacles of the limpet Patella vulgata (L.) are described. The tip of each tentacle is covered by a dense crown of tufts and additional tufts can be seen scattered evenly across the surface of each tentacle. The cilia are nonmotile and nerve fibres run from the base of the ciliated cells suggesting a sensory function. Comparisons are made with ciliary tufts found in a Pacific species of limpet, Acmaea scutum, and other molluscan sensory structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218124
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  • 45
    Electronic Resource
    Electronic Resource
    Effects of melatonin on synaptic ribbons in pinealocytes of the Chinese hamster, Cricetulus griseus (1983)
    Springer
    Cell & tissue research 233 (1983), S. 59-67 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Synaptic ribbon ; Melatonin ; Ultrastructure ; Chinese hamster (Cricetulus griseus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of melatonin on synaptic ribbons (SR) in pinealocytes of the Chinese hamster (Cricetulus griseus) were examined. SR were classified into types 1, 2 and 3, which appear as rods, round or irregular bodies and ring-shaped structures, respectively; a synaptic ribbon index (SR index) was determined for the three types. Administration of two doses of 1.5 mg/kg melatonin at noon and 3 p.m. causes an increase in the type-1 and type-2 SR indices 3 h after the second injection in hamsters kept under alternating light and dark conditions (lights on from 7 a.m. to 7 p.m.). Likewise, in animals that are exposed to extended light for 6 h and receive two doses of melatonin at 7 p.m. and 10 p.m., an increase in the type-1 and type-2 SR indices occurs 3 h after the second injection. The increase in the type-2 SR index induced by melatonin administration to hamsters exposed to extended light is greater than the increase in the type-1 SR index under the same experimental conditions. Type-2 SR index, but not type-1 SR index, increases following bilateral superior cervical ganglionectomy. An increase in type-1 and type-2 SR indices occurs at 6 p.m. in ganglionectomized animals administered two doses of melatonin 6 h (noon) and 3 h (3 p.m.) before the time of sacrifice. No significant change is observed in type-3 SR index in animals subjected to any of the above treatments. The results indicate that exogenous melatonin may act directly on pinealocytes of the Chinese hamster to cause an increase in size and/or number of the type-1 and type-2 SR. Type 3-SR may have a role different from that of type-1 and type-2 SR; type-1 and type-2 SR may be functionally related.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222232
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  • 46
    Electronic Resource
    Electronic Resource
    An ultrastructural morphometric study of the papillary muscle of the right ventricle of the cat (1983)
    Springer
    Cell & tissue research 230 (1983), S. 543-552 
    Details
    ISSN: 1432-0878
    Keywords: Myocardium ; Ultrastructure ; Myocardial biopsy ; Morphometry ; Papillary muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The papillary muscle of the cat heart's right ventricle has not been studied previously with quantitative ultrastructural techniques despite its wide use for functional studies. This tissue was perfusion-fixed, processed for electron microscopy, and morphometric techniques were used to assess the ultrastructural characteristics of the papillary muscle as well as the working myocardial cells. The results of this study were that 73.5% of the papillary muscle was composed of muscle cells, 9.7% of blood vessels, and the remainder of interstitial connective tissue. In the muscle cell the volume fraction of mitochondria was 17.3%, that of myofibrils was 49.8%, and that of the nucleus was 2.0%. The mitochondria to myofibrils ratio was 0.36 and the surface to volume ratio was 0.309. In a quantitative ultrastructural comparison of perfusion and immersion fixed tissue it was found that significant differences in the volume density of the blood vessel lumen existed between the two groups. In addition, there were significant differences in the volume fraction of mitochondria and nucleus between perfusion-fixed and immersion-fixed muscle cells. A concurrent significant decrease between the two groups was also found for the ratio of mitochondria to myofibrils. The perfusion-fixed tissue can be considered to provide only normal baseline data for the papillary muscle of the right ventricle. These data are important as they can be used in future structure-function studies on normal and pathological heart tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216200
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  • 47
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence of variation in the number of secretory granules within the granulosa cells of the sheep corpus luteum (1983)
    Springer
    Cell & tissue research 230 (1983), S. 631-638 
    Details
    ISSN: 1432-0878
    Keywords: Corpus luteum ; Ultrastructure ; Secretory granules ; Hormones ; Quantitative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The granulosa luteal cells of the sheep corpus luteum secrete their hormonal products by exocytosis of granules. Electron micrographs of randomly selected granulosa cells from nine corpora lutea at day 10 of the oestrous cycle were examined to obtain the cellular density of these granules. From the area of the cell, calculated using an x -y digitiser, and the number of granules observed, the number of intracellular secretory granules per μm3 of luteal cell cytoplasm was calculated. There was a large variation in the number of granules per cell within the same corpus luteum and between corpora lutea taken at the same stage of the cycle. The number of intracellular granules in nine corpora lutea varied from 2.12±1.05 granules per μm3 (x, SD, n = 30) cells to 0.36±0.18 granules per μm3 ( x, SD, n = 26). These morphological data suggest that the variation in granule synthesis in individual cells may contribute to the variation in hormone content of corpora lutea at the same stage of the cycle, and the episodic release of hormone into the plasma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216206
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  • 48
    Electronic Resource
    Electronic Resource
    Ultrastructure of synapses with different transmitter-releasing characteristics on motor axon terminals of a crab, Hyas areneas (1983)
    Springer
    Cell & tissue research 231 (1983), S. 103-115 
    Details
    ISSN: 1432-0878
    Keywords: Synapses ; Ultrastructure ; Crustacean ; Neuromuscular synapse ; Synaptic differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic terminals on branches of an excitatory motor axon in a spider crab (Hyas areneas) were examined by electron microscopy to determine whether differences in size, structure, and number of synapses could be correlated with differences in transmitter release. Terminals releasing relatively large amounts of transmitter during low frequencies of nerve impulses (“high-output” terminals) had larger synapses, more prominent presynaptic dense bodies (active zones), and fewer synapses per unit length than terminals releasing relatively small amounts of transmitter (“low-output” terminals). Neither the difference in synaptic area, nor the quantitative differences in the active zones, were sufficient in themselves to explain the difference in synaptic efficacy, and it is postulated that a non-linear relationship may exist between structural features of the synapse and release of transmitter by a nerve impulse, and that differences other than those apparent from the ultrastructure could be involved. Greater facilitation at low-output terminals with high frequencies of nerve impulses may be due to greater reserves of “immediately available” transmitter, and to recruitment or activation of more individual synaptic contacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215778
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  • 49
    Electronic Resource
    Electronic Resource
    Erythropoiesis in the thymus of the spotless starling, Sturnus unicolor (1983)
    Springer
    Cell & tissue research 232 (1983), S. 445-455 
    Details
    ISSN: 1432-0878
    Keywords: Avian thymus ; Intrathymic erythropoiesis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Although previously described in other avian species, intrathymic erythropoiesis is a remarkable feature of the thymus of Sturnus unicolor. In discrete stages of the life cycle of this species, erythroblasts and mature erythrocytes occupy large areas of the thymic cortex and cortico-medullary border. Simultaneously, degenerated thymocytes and epithelial-reticular cells occur in the same areas. The relationship between intrathymic erythropoiesis, degeneration of cortical lymphocytes and epithelial-reticular cells, and macrophage activity is discussed and related to a possible functional role of sex hormones in this phenomenon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213799
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  • 50
    Electronic Resource
    Electronic Resource
    Ciliary epithelia of the mammalian eye in cultured explants (1983)
    Springer
    Cell & tissue research 233 (1983), S. 629-638 
    Details
    ISSN: 1432-0878
    Keywords: Ciliary process ; Cultured explant ; Epithelial cells ; Ultrastructure ; Bovine and rabbit eyes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural characteristics of ciliary epithelium from bovine, pigmented rabbit, and fetal albino rabbit were studied in cultured explants. The tips of ciliary processes were cultured in plastic dishes with Dulbecco Modified Eagle Medium (DMEM) containing 5% fetal bovine serum. More than half of the expiants adhered to the plastic culture dish, and epithelial cells spread as monolayers within a few days. Initially the explant contains two layers, the outer (nonpigmented cells) and the inner (pigmented cells). Later the explant exhibits three layers: 1) outermost lightly pigmented flattened cells, 2) an outer layer of nonpigmented cells, and 3) an inner layer of densely pigmented cuboidal cells. The cells of the outermost layer are continuous with the cells of the inner layer. A narrow space lies between the outermost layer and the outer layer. The columnar cells in the outer layer contain well developed organelles but no pigment granules; they possess a basement membrane, lateral interdigitations, and junctional complexes near their apices. Numerous focal junctions and some ciliary channel-like structures were detected between the columnar cells of the outer layer and the cuboidal cells of the inner layer. The cuboidal cells of the inner layer are filled with pigment granules. These observations suggest that the columnar cells of the outer layer are nonpigmented epithelium, the cuboidal cells of the inner layer are pigmented epithelium, and the flatened cells in the outermost layer are derived from pigmented epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212230
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  • 51
    Electronic Resource
    Electronic Resource
    Halogenated compound response in an oxygen doped ion mobility detector for capillary gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 4-10 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Ion mobility detector ; Plasma chromatography ; Selective detection ; Quantitative analysis ; Halogenated compounds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An ion mobility detector that has been specifically developed for interfacing with capillary gas chromatography is investigated in the negative ion mode. Like the electron capture detector, to which this instrument is closely related, the ion mobility detector shows an enhanced response to low molecular weight halogenated compounds when a small quantity of oxygen is doped into the make-up gas flow. Under O2 doping conditions, the device can operate in a reactant ion monitoring mode responding universally to compounds capable of capturing thermal electrons and in a tunable selective product ion mode providing increased selectivity over that achieved by the ECD. At an oxygen concentration of 0.5%, minimum detectable amounts as low as 600 femtograms have been realized for carbon tetrachloride. Selectivity of chloro- versus bromo- compounds is demonstrated using a mixture of p-dichlorobenzene and p-dibromobenzene.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060102
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  • 52
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    Electronic Resource
    Trace analysis of halocarbons in water; Direct aqueous injection with electron capture detection (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 11-15 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; ECD ; Halocarbons in water, 0.1-10 ppb ; 0.5-2 μl injected on-column ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Interest in monitoring halogenated organics in drinking water and natural surface and ground water in the low ppb range continues to grow. There is a tendency to include still more volatile halocarbons, the trace determination of which is known to be rather demanding. This prompted us to re-examine the feasibility of large-volume direct aqueous injection onto capillary columns, coupled with ECD. A primary problem was to avoid simultaneous elution of water with halocarbons, since water suppresses the ground current of the ECD. The following measures contributed to the solution of this problem.Apolar, extremely inert, columns are required to elute water completely, and even before very light halocarbons. Their coatings have to be far thicker (≍ 5 mUm) than commonly employed thick films since they must permit isothermal analysis at a column temperature around 100°C in order to ensure rapid and complete elution of water. Finally, it is essential that sampling be carried out on-column for two reasons: diffusion of water vapor in the injector, resulting in delayed elution, is then eliminated, and peak distortion during splitless injection is avoided.Although we now know that persilylated columns with immobilized coatings withstand routine water injections, more longterm experience is needed to provide detailed recommendations for the handling of these columns.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060103
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  • 53
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    Electronic Resource
    Derivatives of cholesterol cinnamate: A comparison of the separations of geometrical isomers when used as gas chromatographic stationary phasesMention of a commercial or proprietary product does not constitute an endorsement by the USDA. (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 16-19 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Liquid crystal stationary phases ; para-Substituted cholesterol cinnamate ; Geometrical isomers (insect pheromones) ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Four para-substituted cholesterol cinnamates were synthesized and evaluated for utility as gas chromatograph (GC) liquid crystal stationary phases. The capability of the phases to separate olefinic geometrical isomers was found to be dependent on the position of the olefinic bond. When unsaturation occurred at positions four carbons or greater from the terminal methyl of the compounds investigated, the relative ability of the phases to separate geometrical isomers was p-Cl 〉 p-Me 〉 cholesterol cinnamate 〉 p-MeO derivative 〉 p-NO2. When unsaturation occurred at positions four carbons or less from the terminal methyl the relative ability of the para-derivatives of cholesterol cinnamate to separate geometrical isomers was reversed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060104
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  • 54
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    Electronic Resource
    Dependence of selectivity and resolution upon composition of the binary mobile phase in LSC (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 27-30 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; LSC with binary eluents ; Resolution in LSC ; Selectivity in LSC ; Adsorption LC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The recently derived general equation for the capacity ratio has been utilized to study the dependence of selectivity and resolution upon mobile phase composition. This equation accounts for differences in molecular size of solutes and solvents, and for the energetic heterogeneity of the adscrbent surface. Model calculations illustrating the influence of these factors on the selectivity and resolution are presented and discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060106
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  • 55
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    Electronic Resource
    Aqueous two-layer liquid distribution for chromatographic resolution of nucleoside analogues (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 33-33 
    Details
    ISSN: 0935-6304
    Keywords: Liquid-liquid partition chromatography, HPLC ; Liquid-liquid distribution ; Nucleoside ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060107
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  • 56
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    Electronic Resource
    Effect of water on retention time (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 34-35 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Samples with varying water content ; Retention times correlated with water content ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060108
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  • 57
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    Corrigenda (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 50-50 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060117
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  • 58
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    Preparation of reversed-phase high-performance thin-layer plates by in situ reaction with monochlorosilanes (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 95-100 
    Details
    ISSN: 0935-6304
    Keywords: Thin-layer chromatography, HPTLC ; Reversed phase mode ; Modification of commercially available plates ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The condition for in situ chemical modification of commercially available thin-layer plates have been varied and the influence thereof upon reversed phase plate properties has been studied. The reaction variables studied were: average silica pore size, silica water content, use of tri- or monochlorosilanes as modifiers, reaction temperature, and addition of base. Coverage and activity of residual silanol groups was studied on modified plates.Desired plate properties are high speed of development in aqueous solvents and low content of active residual silanol groups. Under the conditions tested, the lowest amount of residual silanols was achieved at the expence of the speed of development in aqueous media and vice versa.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060209
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    HPTLC analysis of organotin compounds in preservative solutions and preservative-treated wood (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 89-94 
    Details
    ISSN: 0935-6304
    Keywords: Thin-layer chromatography, HPTLC ; Analysis of organotin compounds ; Organotin preservative solutions ; Preservative-treated wood ; Degradation of organotin compounds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A high-performance thin-layer chromatographic (HPTLC) method is described for the determination of tributyltin compounds (bis(tri-n-butyltin) oxide, TBTO, and tri-n-butyltin naphthenate, TBTN) and their degradation products (dibutyltin and monobutyltin compounds). The organotin compounds are extracted from wood with ethanol containing 0.5% (v/v) of hydrochloric acid and the separation of the defferent kinds of organotin compounds is achieved by thin-layer chromatography. The sample spots are measured using a scanning densitometer after decomposing the organotin compounds to inorganic tin by ultraviolet irradiation and visualization of the spots with pyrocatechol violet.Applications of the method to detection and quantification of organotin compounds in preservative solutions, in recently impregnated wood, and in wood samples from five-year-old window frames are described.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060208
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  • 60
    Electronic Resource
    Electronic Resource
    Cutting of glass and fused silica capillaries (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 140-144 
    Details
    ISSN: 0935-6304
    Keywords: Capillary chromatography ; Glass capillary tubing ; Fused silica capillary tubing ; Cutting of glass tubing ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An investigation of techniques suitable for precision cutting of glass and fused silica capillary tubing has been carried out. Accurate trimming of capillary tubing is important when precision splitters and manifolds have to be made. Techniques employed for cutting optical fibers proved to be useful, and factors affecting the suface quality of the fracture are discussed.It was found to be difficult to obtain a square cut with a flat surface in the case of fused silica tubing. The best results were obtained when the tubing was scored with the edge of a broken silicon wafer before the fracture was accomplished. This technique allows precision cutting of glass tubing with an outer diameter of 100 μm or less.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060305
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  • 61
    Electronic Resource
    Electronic Resource
    Optimization via liquid phase mixtures in capillary gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 145-151 
    Details
    ISSN: 0935-6304
    Keywords: Binary phase mixtures ; Coupled capillary columns ; Velocity gradient ; Mixed Liquid phases ; Optimization ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The use of sequentially coupled columns to achieve a binary liquid phase mixture has been simplified by the availability of zero dead volume fittings compatible with fused silica columns. The extent to which the velocity gradient through the coupled column affects the “apparent” liquid phase ratio can be determined by graphic interpolation; the length ratio of the coupled column segments can then be adjusted so that the “apparent” liquid phase ratio actually experienced by the solutes agrees with the targeted value. The fact that the direction of flow through the coupled column affects the chromatographic dispersion suggests that accepted generalizations on flow optimization throughout the column may not be precisely correct.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060306
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  • 62
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    Separation and quantification of epinephrine, norepinephrine, and dopamine by chromatography on diphenyl thin layers (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 156-157 
    Details
    ISSN: 0935-6304
    Keywords: Thin layer chromatography, TLC ; Densitometry ; Catecholamines ; Diphenyl layer ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060308
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  • 63
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    Electronic Resource
    Gradient elution system for capillary and micro HPLC (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 216-217 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; Capillary, fused silica ; Micropacked columns ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060415
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  • 64
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    Behavior of fused silica capillaries subjected to gamma radiation. Part 1: Chromatographic performance (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 241-246 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Fused silica capillary columns ; Cobalt-60 gamma radiation ; Immobilized phase ; Crosslinking ; Column activity ; Temperature Stability ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fused silica capillaries deactivated with D4 and coated with OV-1 were subjected to cobalt-60 gamma radiation in order to elucidate the effect of incident radiation on column performance. The chromatographic performance of these open tubular columns in which OV-1 was polymerized in situ was found to be dependent on the dosage of radiation and was evaluated before and after the irradiated columns were rinsed with solvent. Of the radiation dosages employed, 3 MRad produced superior chromatographic performance coupled with very favorable residual surface activity.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060505
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  • 65
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    Electronic Resource
    Applications of capillary gas chromatography in routine toxicological analyses (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 247-254 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, fused silica ; Screening for basic drugs with retention indices differing by only four units ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Capillary columns offer the toxicologist several advantages over packed columns. We have presented a highly reproducible screening procedure based on the retention index system and have shown the use of capillary columns for routine quantitation. We are currently expanding the use of these columns in our laboratory and predict that they will be in routine use by most toxicologists in the near future.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060506
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  • 66
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    Residue analysis of organophosphorus pesticides in food with two-dimensional gas chromatography using capillary columns and flame photometric detection (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 255-263 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass, fused silica ; Two-dimensional technique ; Residue analysis in food ; Organophosphorus pesticides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Two-dimensional gas chromatography with two capillary columns of different polarity (SP 2100 and OV 225) is used for pesticide residue analysis in food samples. By means of “live chromatography” applying pneumatic switching technique according to Deans, unequivocal identification of 57 organophosphorus pesticides in food samples is achieved at trace concentrations. The instrument is equipped with only one injection port and one flame photometric detector. On-line data processing is very helpful, especially in calibration and checking the system's reliability with the multitude of test compounds. The complete pesticide residue analysis including clean-up of about six food samples can be completed by one person in 8 hours.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060507
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  • 67
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    Evaluation of loop and pump injection systems in preparative liquid chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 264-268 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; Preparative scale chromatography ; Injection mode ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The usefulness of pump or loop injectors in preparative liquid chromatography was evaluated. The concentration distribution of samples flowing through a UV detector cell, directly connected to the injector, was recorded at various flow velocities, at various diameters of loop tubing, and using various methods of injection [injection with sample volumes equal to a fraction or total loop volume, etc.]. The most advantageous methods were found to be either use of a loop injector to inject only a fraction of its total volume or use of a pump. Both of these methods ensure a almost rectangular concentration distribution.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060508
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  • 68
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    An equation for the condition for the basic solvent effect in GLC (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 273-274 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Solvent effect ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060510
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  • 69
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    Analysis of acidic-neutral drugs in human serum by fused silica capillary chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 317-319 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, fused silica ; Drug screening ; Fused silica capillary column ; Toxicology ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060606
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  • 70
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    Solute focusing using the solvent effect: Solute lagging (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 321-322 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060608
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  • 71
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    Product news (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 341-341 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060615
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    Masthead (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983) 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060701
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  • 73
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    Microbore column HPLC (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 348-358 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060702
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  • 74
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    Correlations between HPLC and NMR properties of some selected alkyl bonded phases (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 433-435 
    Details
    ISSN: 0935-6304
    Keywords: Reversed-phase HPLC ; Solid state NMR ; Surface characterization ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060806
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  • 75
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    Simple alternative method for preparing high quality fused silica SE-54 WCOT capillary columns (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 436-440 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, fused silica ; Superox-4 pretreatment, deactivation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060807
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  • 76
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    Electronic Resource
    Slurry packing of high-performance columns for liquid chromatography. Part 1: Effect of quality of tube inner wall (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 440-441 
    Details
    ISSN: 0935-6304
    Keywords: High performance liquid chromatography, HPLC ; Slurry packing ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060808
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  • 77
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    Preparative-scale separation of close isomers and isotope-substituted compounds by high resolution circulation gas chromatography. Part 1: State-tof-the-art (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 460-470 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060902
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  • 78
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    1983 Tswwtt medals awarded to Jellum, Schomburg, Zlatkis (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 492-493 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060906
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  • 79
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    Injection temperature effects using On-column and split sampling in capillary gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 471-479 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; On-column injection ; Split sampling ; Temperature control ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The effect of sample injection temperature on quantitation is examined for on-column and conventional split modes of sampling in capillary gas chromatography. Discrimination effects can be observed even with on-column injection if the injection temperature is too far above the boiling point of the solvent (or that of a major low boiling constituent(. This is attributed to higher boiling components being left behind in the syringe needle, and a set of simulation experiments are described to illustrate this effect. Various discrimination patterns using conventional split injection were observed, depending on temperature of injection. Apart from syringe needle effects, discrimination is probably due to the preferential venting of higher boiling components as liquid sample droplets, which can have a lifetime greater than the time of transit to the splitter. With such a two-phase system, involving variable droplet size, the flow distribution in the splitter will be critical to uniform sampling. The use of combination on-column/split sampling, with the appropriate temperature control to provide sample uniformity to the splitter is discussed as an advantageous alternative to conventional split sampling.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060903
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  • 80
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    Capillary gas chromatography of pesticide residues in foods: Application of a split/splitless inlet system (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 480-487 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column ; Residue mixtures ; Inlet characteristics ; Injection procedure ; Repeatability ; Linearity ; Quantification ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Residue mixtures of pesticides in foods which were difficult to separate by packed-column gas chromatography were determined by capillary gas chromatography and electron capture detection. Manual injections of sample and reference standard were made with a 10 μL syringe on a septum-enclosed, split/ splitless inlet system. Optimal peak height repeatability (± 3%( was achieved with three-μL injections which were made within time periods of four hours or less. The average linearity coefficient (slope( obtained for detector response as a function of solution concentration was 1.1.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060904
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    Elimination of peak splitting and distortion associated with liquid sample on-column injection: Solvent polarity effects (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 497-500 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, fused silica ; On-column injection ; Solvent polarity ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060908
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  • 82
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    On the efficiency of thick film capillary columns (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 501-504 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Van Deemter curves ; Thick film columns ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060909
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  • 83
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    Construction of a polyimide effluent splitter for multiple detection in capillary gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 504-507 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Effluent splitting ; Polyimide ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060911
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  • 84
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    Determination of monosodium glutamate enantiomers by chiral phase capillary gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 510-511 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Optically active compounds ; Determination of trace amounts ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060913
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  • 85
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    Sensitive HPLC determination of atropine with electrochemical detection (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 514-515 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Partition chromatography ; Atropine ; Belladonna alkaloids ; Electrochemical detection ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060915
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  • 86
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    Solute focusing technique for on-column injection in capillary gas chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 554-559 
    Details
    ISSN: 0935-6304
    Keywords: Gas Chromatography ; On-column Injection ; Capillary Columns ; Solute Focusing ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A novel solute focusing technique for on-column injection of liquid samples onto capillary GC columns is described. The focusing technique allows injection of 8.0 microliters or more of sample without producing the peak distortion or splitting observed under conventional on-column injection conditions. The experimentally determined performance of the technique is given for a wide volatility range sample. Solute focusing is useful in situations where on-column injection of 1.0 microliter or greater is required.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061004
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    Improved quantitative capillary GC by the use of CO2 as secondary coolant in cold on-column injectionBased on a paper presented at the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Atlantic City, New Jersey, USA, March 1983 (Paper No. 923). (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 550-553 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, capillary ; Sample discrimination ; Injection, on-column ; Secondary cooling ; Quantitation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A comparison is made between air and carbon dioxide as secondary cooling gases in cold on-column injection into capillary columns. Because CO2 is a more effective coolant than air, the injection zone can be kept at a colder temperature, thus preventing loss of sample vapor through the open injector even as the initial oven temperatures because greater than the boiling point of the solvent. Sample loss at these elevated temperatures is shown as a reduction in peak area as functions of column temperature during injection and different injection zone temperatures. The maximum allowable oven temperature during injection is significantly higher when CO2 is used as secondary coolant rather than air.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061003
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  • 88
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    Technique for accurate measurement of capillary lengths and diameters (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 566-567 
    Details
    ISSN: 0935-6304
    Keywords: Capillary ; Capillary liquid chromatography ; Capillary gas chromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061007
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  • 89
    Electronic Resource
    Electronic Resource
    The solvent effect in gas-liquid chromatography: The analysis of vapor samples (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 567-568 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Solvent effect ; Headspace analysis ; Trace analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061008
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  • 90
    Electronic Resource
    Electronic Resource
    Analysis of bile acids in a serum by micro-HPLC (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 571-572 
    Details
    ISSN: 0935-6304
    Keywords: Micro HPLC ; Bile acids ; Precolumn concentration ; Postcolumn reaction ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061010
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  • 91
    Electronic Resource
    Electronic Resource
    Reversed phase HPLC separation of fossil chlorins (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 573-574 
    Details
    ISSN: 0935-6304
    Keywords: Reversed phase HPLC ; Fluorimetric detection ; Fossil chlorins ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061011
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  • 92
    Electronic Resource
    Electronic Resource
    Guidelines on how to carry out on-column injections (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 581-583 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061014
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  • 93
    Electronic Resource
    Electronic Resource
    Specific retention volumes and retention indices of selected hydrocarbon solutes with OV-101 and SP-2100 polydimethylsiloxane solvents (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 577-580 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061013
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  • 94
    Electronic Resource
    Electronic Resource
    Method of separation and detection of substances on silica gel thin films impregnated with silver nitrate and phosphatotungstic acid (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 575-576 
    Details
    ISSN: 0935-6304
    Keywords: Thin-film chromatography, HPTFC ; Separation on argentized silica gel ; Detection ; Scanning densitometry ; Lipids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061012
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  • 95
    Electronic Resource
    Electronic Resource
    Advances in liquid chromatography (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 583-583 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240061015
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  • 96
    Electronic Resource
    Electronic Resource
    Masthead (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983) 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060101
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  • 97
    Electronic Resource
    Electronic Resource
    Cyanoalkyl-bonded HPTLC plates and their chromatographic characteristics (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 47-48 
    Details
    ISSN: 0935-6304
    Keywords: High performance thin-layer chromatography ; HPTLC bonded phases ; Cyanoalkyl-bonded phase ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060114
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  • 98
    Electronic Resource
    Electronic Resource
    Carrier displacement chromatography for the identification of deprenyl and its metabolites (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 49-50 
    Details
    ISSN: 0935-6304
    Keywords: Thin-layer displacement chromatography ; Carrier displacement ; Deprenyl metabolites ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060115
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  • 99
    Electronic Resource
    Electronic Resource
    Addendum (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 50-50 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060116
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  • 100
    Electronic Resource
    Electronic Resource
    Capillary GC separation of LSD and LAMPA (1983)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 101-103 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, fused silica ; GC/MS ; Drugs of abuse ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240060210
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