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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Der Anaesthesist 47 (1998), S. 320-329 
    ISSN: 1432-055X
    Keywords: Schlüsselwörter Kardiopulmonale Reanimation ; Kammerflimmern ; Defibrillation ; automatisierter Defibrillator ; Impedanz ; biphasische Stromkurven ; Powerspektrumanalyse ; mediane Frequenz ; Frühdefibrillation ; Public Access ; Defibrillation ; Key words Cardiopulmonary resuscitation ; Ventricular fibrillation ; Defibrillation ; Automatic defibrillator ; Impedance ; Biphasic waveform ; Power spectrum analysis ; Median frequency ; Early defibrillation ; Public access defibrillation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Early defibrillation is the standard of care for patients with ventricular fibrillation (VF) and pulseless ventricular tachycardia (VT). Technical developments aim at further miniaturization and simplification of defibrillators as well as adaptation of energy requirements to the patient´s needs. Implantable Cardioverter-Defibrillators (ICD) and automated external defibrillators (AED) are based upon the same technology. Both devices analyze the ECG signal internally, followed by a ”shock” or ”no shock” decision. Use of automated devices is the prerequisite for defibrillation by non-physicians. Chest impedance measurements and use of alternative shock waveforms, such as biphasic, aim at adaptation of energy or current to the patient’s individual needs and avoid application of unnecessarily high amounts of energy to the myocardium. Calculation of median frequency is a non-invasive method for analyzing the heart´s metabolic and electrical state. It helps to determine the optimal moment for defibrillation during cardiopulmonary resuscitation (CPR). Developments concerning the structure of in-hospital emergency systems or pre-hospital emergency medical services (EMS) aim at further reductions in time from collapse of a patient until first defibrillation. Such developments include early defibrillation programs for emergency medical technicians (EMT), nurses, and fire or police department first responders as well as wide distribution of easy-to-operate defibrillators in public areas, as discussed during the American Heart Association’s Public Access Defibrillation conferences. All programs of that kind have to be organized and supervised by a physician who is responsible for training and supervision of the personnel involved.
    Notes: Zusammenfassung In allen internationalen Empfehlungen stellt die schnellstmögliche Defibrillation die Maßnahme der Wahl bei Kammerflimmern (VF) und pulsloser ventrikulärer Tachykardie (VT) dar. Der Defibrillation kommt eine Schlüsselrolle bei der Bekämpfung des plötzlichen Herztodes zu. Technische Neuerungen: Entwicklungen auf technischem Gebiet haben einerseits eine weitere Miniaturisierung und Vereinfachung der Geräte, andererseits eine Anpassung der Energiemenge an die individuellen Patientenbedingungen zum Ziel. Implantierbare Kardioverter-Defibrillatoren (ICD) und automatisierte externe Defibrillatoren (AED) basieren auf der gleichen Technologie, einer geräteinternen Analyse des EKG-Signals mit nachfolgender „Schock-” oder „Kein-Schock”-Entscheidung. Die Verwendung von AED stellt die Grundlage für die Defibrillation durch nicht-ärztliches Personal dar. Die automatische Messung der Thoraximpedanz soll die für die erfolgreiche Defibrillation erforderliche Energie den Bedürfnissen des Patienten anpassen und unnötig hohe Energiemengen vermeiden. In diese Richtung zielen auch alternative Stromkurvenformen, wie die biphasische, bei welcher der Stromfluß nach einem vorgegebenen Intervall die Polarität ändert. Die Bestimmung der medianen Frequenz des VF als nicht-invasives Verfahren zur Bestimmung des metabolischen und elektrischen Zustands des Myokards soll helfen, den optimalen Zeitpunkt für eine erfolgreiche Defibrillation im Verlauf der kardiopulmonalen Reanimation (CPR) festzulegen. Strukturänderungen: Strukturelle Reformen des präklinischen Rettungs- und des innerklinischen Notfallsystems sollen das Zeitintervall vom Kollaps des Patienten bis zur Abgabe der ersten Defibrillation verkürzen. Unter der Maßgabe, daßärztliche Hilfe nicht rechtzeitig zur Verfügung steht, stellt die Defibrillation mittels automatisierter Geräte durch speziell ausgebildetes nicht-ärztliches Personal eine sichere und effektive Methode dar. Sie erfolgt im Rahmen der Notkompetenz des Rettungsassistenten oder von prä- und innerklinischen Frühdefibrillationsprogrammen. In der Diskussion sind momentan sog. First-Responder-Projekte, bei denen z.B. Feuerwehrleute oder Polizei- und Sicherheitspersonal schon vor Eintreffen des Rettungsdienstes mit Hilfe einfacher AED Patienten mit VF defibrillieren. In den USA wird momentan die weite Verbreitung einfachster automatisierter Defibrillatoren, ähnlich Feuerlöschern in öffentlichen Gebäuden, diskutiert.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 160 (1997), S. 127-140 
    ISSN: 1432-1424
    Keywords: Key words:Xenopus oocytes — Calcium channel —β subunit —α2δ subunit — Inactivation — Neuronal cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The α1 subunit coding for the human brain type E calcium channel (Schneider et al., 1994) was expressed in Xenopus oocytes in the absence, and in combination with auxiliary α2δ and β subunits. α1E channels directed with the expression of Ba2+ whole-cell currents that completely inactivated after a 2-sec membrane pulse. Coexpression of α1E with α2bδ shifted the peak current by +10 mV but had no significant effect on whole-cell current inactivation. Coexpression of α1E with β2a shifted the peak current relationship by −10 mV, and strongly reduced Ba2+ current inactivation. This slower rate of inactivation explains that a sizable fraction (40 ± 10%, n= 8) of the Ba2+ current failed to inactivate completely after a 5-sec prepulse. Coinjection with both the cardiac/brain β2a and the neuronal α2bδ subunits increased by ≈10-fold whole-cell Ba2+ currents although coinjection with either β2a or α2bδ alone failed to significantly increase α1E peak currents. Coexpression with β2a and α2bδ yielded Ba2+ currents with inactivation kinetics similar to the β2a induced currents, indicating that the neuronal α2bδ subunit has little effect on α1E inactivation kinetics. The subunit specificity of the changes in current properties were analyzed for all four β subunit genes. The slower inactivation was unique to α1E/β2a currents. Coexpression with β1a, β1b, β3, and β4, yielded faster-inactivating Ba2+ currents than currents recorded from the α1E subunit alone. Furthermore, α1E/α2bδ/β1a; α1E/α2bδ/β1b; α1E/α2bδ/β3; α1E/α2bδ/β4 channels elicited whole-cell currents with steady-state inactivation curves shifted in the hyperpolarized direction. The β subunit-induced changes in the properties of α1E channel were comparable to modulation effects reported for α1C and α1A channels with β3≈β1b 〉 β1a≈β4≫β2a inducing fastest to slowest rate of whole-cell inactivation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of orthopaedic surgery & traumatology 7 (1997), S. 271-275 
    ISSN: 1432-1068
    Keywords: Arthroscopic lateral release ; Patellar compression syndrome ; Section arthroscopique de l'aileron rotulien ; Syndrome fémoro-patellaire
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dans cet article nous présentons la technique, les résultats ainsi que les complications et les indications de la section de l'aileron rotulien externe et la capsulorhaphie interne, à partir des résultats obtenus par l'examen de 7o malades souffrant les uns d'une légère latéralisation rotulienne externe et d'une hyperpression fémoro-patellaire externe (42 malades), les autres d'une luxation recidivante de la rotule (28 malades). La moyenne d'âge était de 26.2 ans (entre 9 et 54 ans). Au moment de l'examen de contrôle (m=64 mois) les malades ont été soumis à un examen clinique et radiologique. Les résultats ont été évalués conformément au score de Crosby et de Insall. 77 % des patients traités par relâchement latéral externe étaient satisfaits du résultat de l'opération (score analogue visuel) et 68 % des patients traités par capsulorhaphie. Par contre seulement 45 % des malades souffrant d'une ar-throse fémoro-patellaire traités par relâchement latéral externe parlaient d'une nette amélioration. Le score dit de Crosby et Insall donnait des résultats très significatifs mais ne montrait aucune différence parmi les résultats obtenus dans ces trois groupes. 13 % des malades souffraient d'un glissement de la rotule anormal en mobilisation active. Le test de glissement passif de la rotule montrait une hyper-mobilité de la rotule vers le côté latéral dans 37 % des cas et vers le côté médial dans 8o % des cas. Les radiographies des deux genoux montraient un angle de sulcus accru de 143 %. Cependant, aucune progression de l'arthrose n'a pu être relevée dans les articulations fémoro-patellaires opérées. Une hémarthrose post-opératoire précoce, nécessitant une révision, a été constatée dans un grand nombre de cas. Intérêt clinique: Vu les résultats sur les malades ayant souffert d'un syndrome d'hyperpression rotulienne sans arthrose patello-fémorale et les malades souffrant d'une luxation ou subluxation récidivante de la rotule, ces patients sont de bons candidats pour une section endoscopique de l'aileron rotulien externe et éventuellement pour capsulorhaphie interne. Dans une population relativement jeune la section endoscopique de l'aileron externe est une technique chirur-gicale relativement simple qui peut néanmoins être suivie par une technique “ouverte” en cas de défaillance de la première procédure.
    Notes: Summary Based on the results of 70 patients with mild lateral tracking and lateral compression of the patella, intact (22 patients) or defective (20 patients) cartilage of the patellofemoral joint, and recurrent patellofemoral dislocation (28 patients) we present the technique, results, complications and indications for the lateral retinacular release and medial imbrication of the vastus medialis obliquus. The average age of the patients was 26.2 years (range 9–54). At time of follow-up (m = 64 months) the patients were examined clinically as well as radiologically. The results were evaluated according to a Crosby and Insall score. 77% of the patients with mild lateral tracking and lateral compression (lateral release) were satisfied with the operative result (visual analog scale), as well as 68% of the patients with recurrent patellofemoral dislocation (lateral release and medial imbrication). Only 45% of the patients with patellofemoral arthritis (lateral release) reported an improvement in their condition after this procedure. The Crosby and Insall score showed significant improvement but did not show any difference between the 3 groups. 13% of the patients showed an abnormal lateral tracking of the patella in active motion. The passive patellar glide test showed patellar hypermobility to the lateral side in 37% of the cases and to the medial side in 80%. X-rays of both knees showed a sulcus angle of 143°. No progression in osteoarthritis in the operated patellofemoral joint was found. Hemarthrosis complicated a high number of cases in the early postoperative period, and in 3 cases an open revision was necessary. Clinical relevance: According to our results patients with patellar compression syndrome without patellofemoral arthrosis and patients with recurrent dislocation or subluxation of the patella are good candidates for endoscopic lateral release and medial imbrication of the medial vastus obliquus. In a young population endoscopic lateral release is a relatively simple surgical procedure making an open technique in failed cases possible later on.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European journal of orthopaedic surgery & traumatology 8 (1998), S. 141-143 
    ISSN: 1432-1068
    Keywords: Osteochondral graft
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Major cartilaginous defects of the femoral condyles of the knee joint, caused by chondropathy and chondral trauma can be a precursor to osteoarthritis. Particularly in cases with large defects the therapy often remains unsuccessful. Even the substitution of articular hyaline cartilage by autogenous osteochondral grafts, regarded as superior to other procedures like Pridies's drilling and abrasion arthroplasty, carries the risk of failure by necrosis of the craft and morbidity at the donor side. We present a 10-year follow-up case who had a large autogenous osteochondral graft from the non-weight bearing periphery of the patellofemoral joint that was implanted after necrosis of the medial femoral condyle following immunsuppressive chemotherapy.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    European journal of orthopaedic surgery & traumatology 8 (1998), S. 61-64 
    ISSN: 1432-1068
    Keywords: ACL rupture ; Arthrotomy ; Meniscectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A group of 94 male and 12 female patients who all suffered from ACL ruptures had a follow-up examination after a period of 14 to 21 years (m = 17.2 years). Each of them had undergone a primary arthrotomy with total or partial meniscectomy. In none of the cases, however, had operative repair of the ACL rupture been performed. The most frequent concomitant injury was a torn medial meniscus which occurred in 74.5% of the cases whereas a damaged lateral meniscus was found in 17.0% of the cases only. Chondromalacia of tibia, femur or patella was observed in 42.5% of the cases. Over 60% of the patients resumed their former sports activities, although in nearly 30% of these cases at a lower level whereas one third of the patients had to practice some other sport or were even forced to give up sports completely. 61.3% still complained about instability of the knee joint, 73.6% of the patients with partial or total meniscectomy had developed osteoarthritis of grade III and IV.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 157 (1998), S. 382-385 
    ISSN: 1432-1076
    Keywords: Key words Chronic hepatitis B ; Children ; Alpha-interferon ; Non-responder ; Retreatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract More than 50% of children with chronic hepatitis B do not respond to treatment with alpha-interferon. Since these patients continue to display high viral replication and progressive liver disease, retreatment should be considered. To date it has not been well evaluated whether a second course of treatment could increase the response rate. In two alpha-interferon retreatment trials in adult patients the response rate, defined by seroconversion from HBeAg to anti-HBe, ranged between 11% and 44%. One beta-interferon retreatment study in children reported a seroconversion rate of 32%. Regrettably, none of the studies included a control group observing the `spontaneous' seroconversion rate after a first interferon cycle. Thus, a nonrandomized alpha-interferon retreatment study in children including control patients was performed. Alpha-interferon for retreatment was administered 3 times a week for 16–24 weeks in 15 children (5–16 years) at least 6 months after ceasing the first cycle. Four children received 5 MU/m2 of a natural alpha-interferon and 11 children 9 MU/m2 recombinant alpha-interferon 2b. Follow up was 18–47 months after initial treatment. In parallel, a control group of 19 un-retreated children with comparable clinical and demographic data was followed for 12–39 months. HBeAg seroconversion was observed in 5 (33%) of the retreated children and in 5 (26%) of the control patients during follow up. The difference is not significant. In the initially nonresponding children, those with high ALT levels before the first treatment showed late HBeAg seroconversion more frequently than those with low ALT levels (P = 0.017) irrespective of retreatment. The ALT level before retreatment was not a predictor for response. Conclusions A second cycle of alpha-interferon during the 3 years following the first treatment in nonresponding children with chronic hepatitis B can be safely performed but did not increase HBeAg/anti-HBe seroconversion compared with the spontaneous seroconversion rate of patients without retreatment.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 60-68 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The octapeptide octreotide crystallizes with three peptide molecules and about 20% water in the asymmetric unit, and in many ways possesses diffraction properties similar to those of a `mini-protein' consisting of 24 amino-acid residues. It diffracts to about 1.0 Å but data in the range 1.4–1.0 Å are weak. It provides a suitable test of different macromolecular X-ray data-collection techniques, especially of their ability to measure weak reflections accurately. In contrast to typical proteins it is possible to perform a full anisotropic refinement, that we believe provides a more objective test of the quality of the data than the internal consistency of equivalent reflections. We have collected a total of six data sets. The X-ray sources included synchrotron radiation, Cu Kα rotating anodes and Mo Kα sealed tubes; position-sensitive two-dimensional detectors from four manufacturers and a four-circle diffractometer with scintillation counter were employed. Two of the six data sets were collected at low temperature. Reasonable anisotropic refinement was possible with all area-detector data sets, although significant differences in the precision of the final model were observed. In addition we tested the ability of automated Patterson interpretation to solve the structure using the six independent data sets. The structure solution was only successful using the synchrotron or rotating-anode data sets, i.e. for the more intense sources. It appears that for structure solution the maximum resolution of the data is critical, whereas for refinement the accuracy of the data is more important.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Type I allergy represents a severe health problem in industrialized countries where up to 20% of the population suffer froin allergic rhinitis, conjunctivitis and allergic asthma bronchiale and in severe cases from anaphylaxis. leading to death.Objective The aim of this study was to evaluate recombinant Bet v I, the major birch pollen allergen for in vivo and in vitro diagnosis of birch pollen allergy.Methods A group of 51 birch pollen allergic patients and eight non-allergic control individuals were tested for birch pollen allergy by skin-prick and intradennal testing, comparing commercial birch pollen extracts with recombinant Bet v I. Quantitative and qualitative serological testing was done with natural and recombinant allergens by radioallergosorbent test (RAST), enzyme-linked immunosorbent assay (ELISA) and immunoblotting.Results Recombinant Bet v I allowed accurate in vivo and in vitro diagnosis of tree pollen allergy in 49/51 patients tested. No false positive results were obtained in any in vitro assay system (ELISA. Westernblot) or by skin testing (skin-prick, intradermal test) with recombinant Bet v I.Conclusion Our results document that recombinant Bet v I produced in bacterial expression systems allows accurate in vitro and in vivo diagnosis of birch pollen allergy in 〉 95% of birch pollen allergic patients.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Voltage-operated Ca2+ channels are heteromultimeric proteins. Their structural diversity is caused by several genes encoding homologous subunits and by alternative splicing of single transcripts. Isoforms of α1 subunits, which contain the ion conducting pore, have been deduced from each of the six cDNA sequences cloned so far from different species. The isoforms predicted for the α1E subunit are structurally related to the primary sequence of the amino terminus, the centre of the subunit (II–III loop), and the carboxy terminus. Mouse and human α1E transcripts have been analysed by reverse transcription–polymerase chain reaction and by sequencing of amplified fragments. For the II–III loop three different α1E cDNA fragments are amplified from mouse and human brain, showing that isoforms originally predicted from sequence alignment of different species are expressed in a single one. Both predicted α1E cDNA fragments of the carboxy terminus are identified in vivo. Two different α1E constructs, referring to the major structural difference in the carboxy terminus, were stably transfected in HEK293 cells. The biophysical properties of these cells were compared in order to evaluate the importance in vitro of the carboxy terminal insertion found in vivo. The wild-type α1E subunit showed properties, typical for a high-voltage activated Ca2+ channel. The deletion of 43 amino acid residues at the carboxy terminus does not cause significant differences in the current density and the basic biophysical properties. However, a functional difference is suggested, as in embryonic stem cells, differentiated in vitro to neuronal cells, the pattern of transcripts indicative for different α1E isoforms changes during development. In human cerebellum the longer α1E isoform is expressed predominantly. Although, it has not been possible to assign functional differences to the two α1E constructs tested in vitro, the expression pattern of the structurally related isoforms may have functional importance in vivo.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Torsatron and stellarator plasma devices rely on magnetic surface mapping to verify the detailed vacuum magnetic field structure. Two new techniques of magnetic surface mapping are under development on the compact Auburn torsatron. The first is a fast-rotating fluorescent wire (FRFW) system. Compared with the usual fluorescent screen, fluorescent rod, or the capacitive point probe techniques, the FRFW system has the advantage of greatly increasing the number of detectable toroidal transits and of minimizing surface mapping time. The second technique is a resistive wire system which provides an alternative fast-field mapping method. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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