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  • 1
    Digitale Medien
    Digitale Medien
    A new compact electron spin polarimeter with a high efficiency (1997)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 68 (1997), S. 4390-4395 
    Details
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: We have developed a new compact retarding-potential Mott spin polarimeter and achieved an efficiency of 1.9×10−4 for gold target operating in 25 keV. A novel design of the retarding field electron optics with 0.59 sr collection solid angle for scattered electrons was adopted based on Monte Carlo calculations for the spin-dependent electron scattering process and electron beam ray-tracing calculations. We have combined the new spin polarimeter with an angle-resolved photoelectron spectrometer and measured the spin- and angle-resolved photoelectron spectra and studied the spin-dependent electronic structure of Ni(110) along the Γ¯S¯ line of its surface Brilluoin zone. © 1997 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1148401
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  • 2
    Digitale Medien
    Digitale Medien
    Design of the cell and gas-handling system for the x-ray absorption study of supercritical fluid material : Proceedings of the 5th international conference on synchrotron radiation instrumentation (1995)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 66 (1995), S. 1437-1439 
    Details
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: A cell and gas-handling system of the supercritical material for x-ray absorption measurement are designed. The cell was made of stainless steel with a pair of Be windows of 3 mm thickness. The x-ray path can be controlled by changing the distance between two Be windows. Electric cartridge heaters are embedded in the cell to control the temperature. With this system we have studied the supercritical state of CF3Br, whose critical point is 67 °C and 39.1 atm. The results of x-ray absorption fine structure measurements for Br K edge for gaseous, liquid, and supercritical states of this material are presented together with the performance of the gas-handling system. © 1995 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1145932
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  • 3
    Digitale Medien
    Digitale Medien
    Reaction of SiC+n (n=1–6) and SiC−n (n=2–4) with O2 molecules in a radio frequency ion trap (1995)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 103 (1995), S. 9963-9967 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: Ion-molecule reactions of silicon-carbon binary cluster ions SiC+n (n=1–6) and SiC−n (n=2–4) with O2 molecules were studied by using an rf ion trap. For all selected positive and negative parent ions, only SiCnO+ or SiCnO− were produced, respectively. The rate constants for the oxidation of these clusters were determined for the first time. The size dependence of the reactivities are discussed, and the presence of the ring form positive ions for n=3 and 5 are suggested. © 1995 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.469885
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  • 4
    Digitale Medien
    Digitale Medien
    A Rapid Method for the Separation of Functional Lymphoid Cell Populations of Human and Animal Origin on PVP-Silica (Percoll) Density Gradients (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 10 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Subclasses of lymphocytes can be separated on gradients of non-toxic polyvinylpyrrolidone-coated colloidal silica (Percoll) by virtue of differential densities. Such gradients can yield functionally active lymphocyte populations after brief centrifugation. Gradients can he generated in a discontinuous step fashion and centrifuged in standard table-top laboratory centrifuges or as self-generating gradients duriny ultracentrifugation. The density medium has low viscosity and can be made isotonic for virtually any use. Gradients have proved useful in both human and experimental animal studies, and high percentage yields allow for separations from small cell numbers. Methods are described for separation of whole blood and lymphotd subpopulations. including blasts stimulated with mitogens or in mixed lymphocyte reactions. The cytotoxic capability of various density fractions was evaluated for mixed lymphocyte culture-induced allogeneic killing and spontaneous, so-called ‘natural’ killer cell activity. The lower density associated with blast transformation allows for significant enrichments of stimulated cells from in vitro cultures. Higher thymidine incorporation, restimulation in mixed lymphocyte reactions, and greater cytotoxic capacity are associated with these‘blast’fractions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb01391.x
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  • 5
    Digitale Medien
    Digitale Medien
    Evidence of ‘K’-Cell Killing by Alloactivated, Fc-Receptor-Bearing Cytotoxic T Lymphocytes (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Upon in vivo alloactivation of Ig-anti-Ig column-purified splenic ‘T’ cells in lethally irradiated allogeneic recipients, a variable proportion of donor-derived cytotoxic T lymphocytes (CTLs) are able to bind IgG antibody-coated erythrocytes through surface Fc receptors (FcR) and form rosettes. The use of fractionation procedures based on the ability of these cells to form rosettes has enabled us to separate FcR-positive CTLs from FcR-negative CTLs and to examine the ability of these two cell populations to perform as effector cells in direct T-cell-mediated killing and in antibody-dependent cellular cytotoxicity. A series of experiments, either by direct isolation of the two cell populations or by deletion of the FcR-positive population by filtration through complexed immunoglobulin columns (Ig-anti-Ig), has shown both populations to be efficient in direct T-cell mediated cytotoxicity against the relevant target cell. The striking difference between the two populations is the exclusive ability of the FcR-positive population to function as effector cells in antibody-dependent cellular cytotoxicity (ADCC). Purification steps before in vivo alloactivation of our responding cells for the removal of ‘B’ cells and FcR-bearing cells with‘K’-cell activity, followed by procedures to remove phagocytic and adherent cells in the resulting immune spleen cell preparation and, finally, by velocity sedimentation of the rosetting and nonrosetting blasts from the small lymphocyte population, has resulted in a population of FcR-positive cells 98% positive for the Thy 1.2 alloantigen. These fractionation steps and immnunofluorescence criteria of purity strongly favor the contention that the ADCC activity within the FcR-positive T-cell population is indeed a property of the CTL itself.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02152.x
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  • 6
    Digitale Medien
    Digitale Medien
    Comparison of a multi-allergen dipstick IgE assay to skin-prick test and RAST (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 20 (1990), S. 0 
    Details
    ISSN: 1365-2222
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: A multi-allergen dipstick enzyme immunoassay ‘Quidel Allergy Screen’ (QAS) has recently been developed commercially for measuring IgE antibodies against nine allergens (house dust 1, house dust 2, Dermatophagoides pteronyssinus, D. farinae, Japanese cedar, ragweed, cat dander, sweet vernal grass, and egg white) at one time. To assess whether this assay is useful in screening allergen-specific IgE antibody, we compared the titres of IgE antibodies against the nine allergens measured by QAS to those in the skin-prick test and by RAST in 93 atopic asthmatics and 22 normal subjects. We found a good overall agreement between the results of the skin-prick test and the results of QAS (sensitivity = 47.1-81.4%, specificity = 84.5-100%, and agreement =78.9-88.9%). The sensitivities against house dust I, D. pteronyssinus. and D. farinae ranged from 77.2 to 81.4%. However, the sensitivities against house dust 2, Japanese cedar, ragweed, and cat dander were low (47.1-68.8%). We also found a good overall agreement between the results of RAST and the results of QAS, except for egg white (sensitivity = 46.2-94.4%, specificity = 87.4-100%, and agreement = 77.4-96.5%). The sensitivities against house dust 1 and 2, D, pteronyssinus, D. farinae, and Japanese cedar ranged from 86.0 to 94.4%. The sensitivities against ragweed, cat dander, and sweet vernal grass were low (46.2-52.6%). There were strong correlations between the titres of RAST and the titres of QAS except cat dander and egg white (r= 0.701 0.924 for the seven allergens). Thus, we conclude that QAS is useful in screening IgE antibodies against multiple allergens at one time. However, because the sensitivities against some allergens tested were low, further improvement of some allergen preparations seems to be necessary in the assay.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2222.1990.tb02664.x
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  • 7
    Digitale Medien
    Digitale Medien
    GLYCOSURIA AND INSULITIS IN NOD MICE EXPRESSING THE HLA-DQw6 MOLECULE (1989)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 16 (1989), S. 0 
    Details
    ISSN: 1744-313X
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: To investigate HLA-linked genes controlling the susceptibility and resistance to insulin dependent diabetes mellitus (IDDM), HLA-DQ alleles of 45 Japanese patients with IDDM were analysed, using sequence specific oligonucleotide (SSO). DQA1*0301 and DQBI*04 were positively associated (R.R = 6.6, Pc〈0.05 and R.R. = 4.7 Pc〈0.01) and DQAI*0103 and DQBI*0104 were negatively associated (R.R. =0–2, Pc〈0.01) with IDDM. DQAI*0103 and DQB1*0104 were in strong linkage disequilibrium to encode for DQw6 molecule. Therefore, in a Japanese population, the DQw6 molecule seems to control the resistance to IDDM. To determine whether or not the DQw6 molecule itself can protect against glycosuria and insulitis in NOD mice, these animals were mated with HLA-DQw6 transgenic-C57BL/6 mice (DQw6-B6) and the F1 progeny expressing the DQw6 molecule were backcrossed with NOD mice. Eighty-five female backcross progenies were classified into four groups, according to the MHC classII phenotype; I-Anod/I-Anod DQw6(-), I-Anod/I-Anod DQw6(+), I-Anod/I-Ab DQw6(-) and I-Anod/I-Ab DQw6(+). At the age of 16 weeks, 9.1% of the DQw6(-) I-Ab(-) mice had a glycosuria whereas none of the DQw6(+) I-Ab(-) mice had a glycosuria. At the age of 30 weeks 13.6% of the DQw6(-) I-Ab(-) mice had a glycosuria and 7.7% of the DQw6(+) I-Ab(-) mice had a glycosuria. Histological examinations of the pancreas were performed in the 30 week old mice or after the development of glycosuria. About 50% of I-Ab(-) mice developed insulitis, regardless of the expression of the DQw6 molecule. Thus, the DQw6 molecule seemed to delay the onset of glycosuria but did not protect against glycosuria and insulitis in the NOD mice despite a functional expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1744-313X.1989.tb00493.x
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  • 8
    Digitale Medien
    Digitale Medien
    Molecular cloning and nucleotide sequence of tuna growth hormone cDNA
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 949 (1988), S. 35-42 
    Details
    ISSN: 0167-4781
    Schlagwort(e): (Tuna) ; Growth hormone ; Nucleotide sequence ; cDNA library
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(88)90051-6
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  • 9
    Digitale Medien
    Digitale Medien
    Immunohistochemical staining of cutaneous tumours with G-81, a monoclonal antibody to dermcidin (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 151 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Background  Recently, the novel antimicrobial peptide named dermcidin (DCD) was reported in human eccrine sweat glands.Objectives  We investigated the expression of DCD in a variety of cutaneous tumours in order to assess the usefulness of the monoclonal antibody (G-81), which recognizes a fragment of DCD.Patients/methods  We studied the immunoreactivity of the G-81 antibody on 197 cutaneous tumours.Results  A total of 13 of 26 cutaneous mixed tumours showed substantial immunoreactivity. In contrast all the following cases were completely unreactive: (i) epithelial tumours (seborrhoeic keratosis, squamous cell carcinoma, Bowen's disease, actinic keratosis, genital Paget's disease); (ii) follicular tumours (basal cell carcinoma, trichilemmoma, trichoepithelioma, trichoblastoma, keratoacanthoma, proliferating trichilemmal tumour, pilomatricoma); (iii) melanocytic tumours (malignant melanoma, naevus cell naevus, Spitz naevus, blue naevus); (iv) neural tumours (schwannoma, neurofibroma, Merkel cell neoplasm); (v) mesenchymal tumours (soft fibroma, dermatofibroma, dermatofibrosarcoma protuberans, vascular leiomyoma, leiomyosarcoma, lipoma, juvenile xanthogranuloma, angiomyoma); and (vi) other sweat gland tumours (poroid neoplasms, syringoma, cylindroma, clear cell hidradenoma, spiradenoma, syringoid eccrine carcinoma, mucinous carcinoma, apocrine cystadenoma, syringocystadenoma papilliferum, apocrine adenocarcinoma). Twenty-six cutaneous mixed tumours were considered from histopathological findings to be the apocrine type, but 13 of 26 mixed tumours contained some DCD-immunopositive cells that possibly differentiate into eccrine secretory glands.Conclusions  We found the expression of DCD in tubular structures of 50% of cutaneous mixed tumours with apocrine differentiation. These results suggest that a number of cutaneous mixed tumours show both eccrine and apocrine differentiation in the same neoplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.06079.x
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  • 10
    Digitale Medien
    Digitale Medien
    Purification and characterization of Contractin A from the pedicellarial venom of sea urchin, Toxopneustes pileolus
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 284 (1991), S. 279-284 
    Details
    ISSN: 0003-9861
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0003-9861(91)90296-U
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