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  • 11
    Electronic Resource
    Electronic Resource
    Combined effects of NO inhalation and intravenous PGF2α on pulmonary circulation and gas exchange in an ovine ARDS model (1996)
    Springer
    Intensive care medicine 22 (1996), S. 656-663 
    Details
    ISSN: 1432-1238
    Keywords: Adult respiratory distress syndrome ; Nitric oxide ; Sheep ; Lung lavage ; Pulmonary circulation ; Pressure-flow relationship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives Inhalation of nitric oxide (NO) selectively dilates pulmonary vessels in well-ventilated regions. Prostaglandin F2α (PGF2α) is a vasoconstrictor and is reported to enhance hypoxic pulmonary vasoconstriction. The objective of this study was to examine whether the combination of intravenous PGF2α and inhaled NO in ARDS lungs has a beneficial effect on oxygenation. Design We investigated the effect of intravenous PGF2α infusion (0.05–10.0 μg/kg per min) with and without NO inhalation (60 ppm) on the hemodynamics and gas exchange in an ovine ARDS model, examining the pulmonary artery pressure versure the flow plot by varying cardiac output. Measurements and results After lung lavage, NO inhalation reduced the mean pulmonary arterial pressure (MPAP) by decreasing the zero-flow pressure intercept from 10.6±3.8 (mean±SD) to 8.5±3.8 mmHg (p〈0.05) with no significant change in slope. NO inhalation improved PaO2 from 56±12 to 84±38 mmHg (p〈0.005) and reduced pulmonary shunt from 65±5 to 53±8% ( $$\dot Qs/\dot Qt$$ ) (p〈0.001). The dose-dependent effects of PGF2α infusion were: (1) increased MPAP attributed to an increased slope in pulmonary artery pressure-flow plot; (2) decreased cardiac index; (3) decreased $$\dot Qs/\dot Qt$$ with unchanged PaO2. The dose-dependent decrease in $$\dot Qs/\dot Qt$$ after PGF2α infusion was attributed to the decreased cardiac output. Conclusions It is suggested that inhalation of NO reduced the critical vascular pressure near alveoli without affecting upstream vessels, while infused PGF2α constricted the larger upstream pulmonary artery vessels without appreciably affecting the critical pressure. Inhalation of NO into well-ventilated lung areas shifted perfusion to well-oxygenated areas, and there was no supplemental shift in blood flow by adding an infusion of PGF2α.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01709742
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  • 12
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of type V collagen in normal human skin (1988)
    Springer
    Archives of dermatological research 280 (1988), S. 145-151 
    Details
    ISSN: 1432-069X
    Keywords: Immunohistochemical localization ; Type V collagen ; Normal human skin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tissue distribution of type V collagen in normal human skin was studied using an indirect immunofluorescent technique to determine whether type V collagen is present in the interstitium or in the basement membrane. Type V collagen was isolated from the human placenta by pepsin digestion and was purified with fractioning salt precipitations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that type V collagen contained α1(V) and α2(V) chains, but not the α3(V) chain. Specificity of the rabbit antibodies to type V collagen was assessed using enzyme-linked immunosorbent assay (ELISA) and an immunoblotting method. Antibodies showed no cross-reactivity to other collagens, laminin, and fibronectin. With an indirect immunofluorescent technique, type V collagen was found to be widely distributed throughout the dermis. Intense fluorescent staining was noted in the papillary dermis and adnexal dermis surrounding hair follicles and eccrine glands. The basement membrane of the dermoepidermal junction, skin appendages, and capillaries was not stained. By indirect immunoperoxidase double staining, type V collagen was not found to be deposited on type IV collagen present in the basement membrane. Immunoelectron microscopic studies showed that type V collagen was not located in the basal lamina. These results suggest that type V collagen is distributed in the interstitium, but not in the basement membrane of normal human skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00456844
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  • 13
    Electronic Resource
    Electronic Resource
    Enhanced expression of ras gene products in psoriatic epidermis (1988)
    Springer
    Archives of dermatological research 280 (1988), S. 259-263 
    Details
    ISSN: 1432-069X
    Keywords: ras gene product ; Psoriasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ras oncogene product ras p21 is structurally homologous to guanine nucleotide-binding proteins and plays an important role in transducing signals elicited by membrane receptors into intracellular metabolism. We examined psoriatic tissues for expression of ras p21 and compared them with normal skin, using the indirect immunofluorescence technique with the anti-ras p21 monoclonal antibody (MoAb), rp-35. In normal epidermis of five healthy individuals and uninvolved epidermis of three psoriatic patients, only the basal layer was positively stained by rp-35. The spinous layer was negative or faintly positive. In contrast, all psoriatic epidermis obtained from 13 psoriatic patients had strong reactivity with rp-35 throughout the epidermis. There were no differences in the staining pattern of hair follicles, sebaceous glands, eccrine glands, and eccrine ducts, which positively reacted with rp-35, between psoriatic and normal skin. The functions of ras p21 have not been clearly identified in mammalian cells; however recent reports reveal that cyclic AMP production is inhibited by the transfection of activated ras gene into normal cells. Enhanced expression of ras p21 in psoriatic epidermis may be indicative of some mechanism of defective β-adrenergic responsiveness, which is considered to be one of the important pathophysiological phenomena causing the hyperproliferative condition in psoriasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440597
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  • 14
    Electronic Resource
    Electronic Resource
    Structural and immunochemical characterization of β-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of Candida glabrata (1998)
    Springer
    Archives of microbiology 169 (1998), S. 188-194 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsCandida glabrata ; Cell wall mannan ; Polysaccharide antigen ; Antigenic factor ; Chemical ; structure ; β-1 ; 2 Linkage ; Phosphodiester ; 13C nuclear ; magnetic resonance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A mannan of Candida glabrata IFO 0622 digested by Arthrobacter exo-α-mannosidase and a β-1,2-linked mannobiose obtained from the parent mannan by acid treatment was analyzed using 13C nuclear magnetic resonance spectroscopy. The results show that the β-1,2-linked mannobiosyl residue is esterified to a phosphate group through position C-1 in the α-configuration, Manβ1– 2Manα1–HPO3–. The results of immunochemical assays of these mannans using the commercial antigenic factor sera of the genus Candida (Candida Check, Iatron) indicate that the main recognition site of serum no. 6 in this kit is the mannotetraosyl side-chain Manβ1–2Manα1– 2Manα1–2Man in C. glabrata mannan and also suggest that the phosphate-containing unit (such as Manβ1– 2Manα1–HPO3– in this mannan) behaves as one of the antigenic determinants of serum no. 6, but not of serum no. 5. Therefore, the present and previous findings indicate that serum no. 5 recognizes relatively longer β-1,2-linked oligomannosyl side-chains, Manβ1–[2Manβ1–]n 2Man (n = 1–6), attached to the phosphate groups previously observed in the cell wall mannans of Candida albicans, Candida stellatoidea, and Candida tropicalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050559
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  • 15
    Electronic Resource
    Electronic Resource
    A case of melorheostosis with a 14-year follow-up (1995)
    Springer
    European radiology 5 (1995), S. 651-653 
    Details
    ISSN: 1432-1084
    Keywords: Melorheostosis ; Bone scintigraphy ; Radiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Melorheostosis is a benign sclerosing dysplasia with a very unusual and characteristic roentgenographic appearance. We reported a patient with melorheostosis in the left lower extremity followed up for 14 years. Although the extraosseous lesions only slightly enlarged, the bone scintigraphy showed the activity of the metabolism to be still high.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190935
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  • 16
    Electronic Resource
    Electronic Resource
    A Slow-Positron Source Project Using the Photon Factory Electron Linac (1992)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 105-110 (Jan. 1992), p. 1833-1836 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/59/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.105-110.1833.pdf
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  • 17
    Electronic Resource
    Electronic Resource
    The influence of total body hyperthermia on brain haemodynamics and blood-brain barrier in dogs (1995)
    Springer
    Acta neurochirurgica 135 (1995), S. 62-69 
    Details
    ISSN: 0942-0940
    Keywords: Total body hyperthermia ; autoregulation ; blood-brain barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was designed to examine the influence of total body hyperthermia (TBHT) using an extracorporeal circuit with a heat exchanger on the cerebral blood flow (CBF), intracranial pressure (ICP), brain tissue pH, cerebral autoregulation and blood-brain barrier (BBB) permeability in dogs. The rectal temperature of the dow was raised to 41.5 °C, maintained at 41.5–42.0 °C for 2 hours (HT period) and then reduced to normothermia by cooling. Regional CBF was measured by the hydrogen clearance method before heating, during the HT period and after cooling. ICP and brain tissue pH were monitored during the TBHT treatment. Autoregulation of the CBF during the HT period was assessed by measuring the regional CBF and the ICP in a state of induced hypo- or hypertension. The influence of TBHT on BBB permeability was examined using an immunohistochemical technique. The regional CBF increased from 38.1±6.5 (mean±SD) to 49.1±9.8 ml/100 g/min and the ICP from 10.3±4.2 to 16.8±3.4 mmHg when TBHT was raised. These returned to normal values after cooling. The regional CBF and the ICP changed in parallel with drug-induced changes of mean arterial blood pressure during the HT period. These changes suggest that autoregulation of the CBF is paralysed during the HT period. Brain tissue pH decreased rapidly when the rectal temperature exceeded 41.0 °C. The pH was 7.18±0.05 during the HT period and was relatively stable. The pH returned to a normal value after cooling. Immunopositive stain for albumin was not observed in heated brain tissue except for the normally leaky pineal gland and the choroid plexus, indicating preservation of BBB during TBHT. These results suggest that brain oedema may occur easily due to paralysed cerebral autoregulation when the arterial blood pressure fluctuates excessively, so arterial blood presssure must be controlled strictly during TBHT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02307416
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  • 18
    Electronic Resource
    Electronic Resource
    Primary intracranial arachnoid cyst in the elderly: A survey on 39 cases (1991)
    Springer
    Acta neurochirurgica 113 (1991), S. 42-47 
    Details
    ISSN: 0942-0940
    Keywords: Primary intracranial arachnoid cyst ; elderly ; clinical analysis ; management ; outcome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Thirty-nine cases of primary intracranial arachnoid cyst in the elderly, including one case of our own, were analyzed. Clinical characteristics in these patients were as follows. (1) The number of patients decreased with age, but there were no differences according to sex. (2) Clinical manifestations were similar to those of chronic subdural haematoma or normal pressure hydrocephalus including dementia, urinary incontinence, and hemiparesis. General symptoms such as headache and seizures were also present. (3) Surgery was performed in most patients with generally good outcome regardless of operative procedures (capsular resection vs. shunt). (4) In some cases of advanced age, disease manifestation may have been due to slight head injuries. Our own case is described as an example.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01402113
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  • 19
    Electronic Resource
    Electronic Resource
    Transient cerebral ischaemia in mongolian gerbils pre-exposed to hypoxia (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 678-683 
    Details
    ISSN: 0942-0940
    Keywords: Cerebral ischaemia ; hippocampus ; hypoxia ; delayed neuronal death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The objective of this study was to clarify whether pre-exposure to hypoxia influences neuronal death following transient cerebral ischaemia. Twenty gerbils were exposed to 10% oxygen in a chamber for 3 weeks. The other control gerbils (n = 20) were fed in normoxia for 3 weeks. Both carotid arteries in the neck were occluded with aneurysm clips for 5 minutes under halothane anaesthesia in 30 gerbils, recirculated and then fed in normoxia. Five animals in both groups were sacrificed before, and 2, 4, and 7 days after surgery. The animals were fixed with 4% paraformaldehyde and histological study was performed. Immunohistochemical study was also done with antibodies against basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). The neuronal death in the hippocampus was more severe in the hypoxic group. Expression of both bFGF and VEGF was obvious in the cingulate cortex, corpus callosum and internal capsule before clipping in the hypoxic group, but not observed in the normoxic group before clipping. We observed the expression of both bFGF and VEGF widely in the brain at 2 and 4 days after recirculation in both groups. The expression in the hypoxic group was much more prominent than that in the normoxic group. These expressions were not observed at 7 days in both groups. Pre-exposure to hypoxia followed by transient cerebral ischaemia accelerated neuronal death in the hippocampus, and induced the more obvious expression of both VEGF and bFGF compared with those in the normoxic group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01412004
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  • 20
    Electronic Resource
    Electronic Resource
    Alterations of Calcium/Calmodulin-Dependent Protein Kinase II Activity in Ischaemia-Induced Neuronal Death and Neuronal Protection Against Ischaemia in the Gerbil Hippocampus (1999)
    Springer
    Acta neurochirurgica 141 (1999), S. 287-294 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Transient forebrain ischaemia; delayed neuronal death; neuronal protection; calcium/calmodulin-dependent protein kinase II.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary  To clarify the relation between neuronal protection against ischaemia and calcium/calmodulin-dependent protein kinase II (CaM kinase II) activity, we investigated temporal alterations of the kinase activity in the hippocampus after transient forebrain ischaemia under neuroprotective conditions, employing the gerbil bilateral carotid artery occlusion model.  The hippocampal CA1 neuronal density at 2 hours after 5 minutes of forebrain ischaemia was 214.7±25.8 /mm (mean±S.D.), and did not differ from the control significantly; however, it decreased to 11.7±4.2 /mm at 7 days after the ischaemia. The neuronal density at 7 days after the ischaemia was 185.1±18.5 under the hypothermic conditions, 128.7±19.6 with the brief ischaemic pretreatment, 65.0±13.4 with administration of MK-801, and 20.5±4.2 with the repetitive hyperthermic pretreatment, respectively.  The Ca2+/calmodulin-dependent activity of CaM kinase II in the hippocampal cytosolic fraction was decreased to 47.5% of the control value at 2 hours after the ischaemia, when CA1 neuronal death was not observed. In contrast, the activity was 98.8% of the control under the hypothermic conditions, 91.4% with the brief ischaemic pretreatment, 71.2% with administration of MK-801, and 47.9% with the repetitive hyperthermic pretreatment, respectively.  These results indicated that the preservation of the Ca2+/calmodulin-dependent activity of cytosolic CaM kinase II after ischaemia parallelled the neuroprotective effect in the gerbil hippocampus. Thus, it is suggested that the preservation of the activity may be involved in the mechanism of neuronal protection against ischaemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050300
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