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  • Electronic Resource  (8,501)
  • 1980-1984  (4,650)
  • 1975-1979  (3,851)
  • Analytical Chemistry and Spectroscopy  (7,572)
  • Electron microscopy  (929)
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  • Electronic Resource  (8,501)
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  • 1
    Electronic Resource
    Electronic Resource
    Epithelium of the human chorion laeve in diabetes mellitus (1984)
    Springer
    Virchows Archiv 402 (1984), S. 289-296 
    Details
    ISSN: 1432-2307
    Keywords: Epithelium ; Chorion laeve ; Diabetes mellitus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The epithelium of human chorion laeve from pregnancies complicated by diabetes mellitus was examined under the light and electron microscopy. In comparison with normal chorion laeve, the epithelium of chorion laeve in diabetes mellitus shows the following morphological changes: 1. The trophoblast cells display more microvilli and cell processes on their surface. 2. Trophoblast cells with different cytoplasmic characteristics can be seen. Some display few organelles and large glycogen depositions; other are rich in organelles. Furthermore, some cells with electron dense filaments and few cell organelles can be found. 3. Necrotic cells are frequently present in the outer epithelial layers. 4. The intercellular spaces in the epithelial layers near the basement membrane are narrower. Since vasculopathy of the decidual arteries with disturbance of blood circulation is often found in pathological pregnancies, we assume that the morphological changes found are due to deficiency in nutrient and oxygen supply.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695082
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure and membrane permeability of cultured pancreaticβ-cells exposed to alloxan or 6-hydroxydopamine (1984)
    Springer
    Virchows Archiv 404 (1984), S. 31-38 
    Details
    ISSN: 1432-2307
    Keywords: Alloxan ; Culture ; Electron microscopy ; 6-Hydroxydopamine ; Pancreaticβ-cells ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Stereological techniques on electron microscopy micrographs were used to evaluate the morphological changes of cultured isletβ cells that had been exposed to alloxan or 6-hydroxydopamine. Trypan Blue exclusion by cells cultured for 3 days indicated that the cells were 100% viable. Electron microscopy revealed that nearly all of the surviving cultured cells wereβ cells. Exposure to 5 mmol/l alloxan or 1–5 mmol/l 6-hydroxydopamine for 10 or 30 min caused a general swelling of the cultured cells with a concomitant swelling of mitochondria and nuclei. The size of the secretory granules was not affected by the drugs. Only 3–10% of the cells excluded Trypan Blue after exposure to 5 mmol/l alloxan or 6-hydroxydopamine. The data conform with the hypothesis that a primary action of alloxan and 6-hydroxydopamine is at the plasma membrane level ofβ cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00704248
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  • 3
    Electronic Resource
    Electronic Resource
    Small cell neuroendocrine (oat cell) carcinoma of the male breast (1984)
    Springer
    Virchows Archiv 404 (1984), S. 213-221 
    Details
    ISSN: 1432-2307
    Keywords: Oat cell carcinoma ; Male breast ; Immunocytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of small cell neuroendocrine (oat cell) carcinoma of the breast in a 52-year old male is presented. Oat cell carcinomas have been reported in various extrapulmonary sites, but this is the second case of a primary oat cell carcinoma of the breast and the first one to have been documented in a male. The tumor was investigated histologically, immunocytochemically and ultrastructurally. The relationship to so-called “carcinoid” mammary tumors is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00704065
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  • 4
    Electronic Resource
    Electronic Resource
    Elephantiasis neuromatosa (1984)
    Springer
    Virchows Archiv 404 (1984), S. 427-434 
    Details
    ISSN: 1432-2307
    Keywords: Elephantiasis neuromatosa ; Neurofibromatosis ; Electron microscopy ; Immunohistochemistry ; S-100 protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An example of elephantiasis neuromatosa, an unusual variant of neurofibromatosis, coexistent with lipomatosis is presented. This dual cell population produced a diffuse swelling of the gluteal sulcus of a young female. The neurogenous origin of the spindle cell component was substantiated by immunoreactivity for S-100 protein as well as by the fine structural observation of a regular basement membrane coating and a profusion of Luse bodies. As far as can be ascertained, this is the first reported immunohistochemical and ultrastructural study of this particular variant of peripheral nerve sheath lesion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695226
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  • 5
    Electronic Resource
    Electronic Resource
    Histoenzymological and ultrastructural study of a bifocal calcifying epithelial odontogenic tumor. characteristics of epithelial cells and histogenesis of amyloid-like material (1984)
    Springer
    Virchows Archiv 403 (1984), S. 67-76 
    Details
    ISSN: 1432-2307
    Keywords: Calcifying epithelial odontogenic tumor ; Electron microscopy ; Histoenzymology ; Ameloblast ; Amyloid-like substance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A calcifying epithelial odontogenic tumor, simultaneously located in the two jaws (maxilla and mandible) was examined by histochemical and electron microscopic methods. Squamous tumor cells without secretory polarity were different from those of common ameloblastoma. High activities of alkaline phosphatase and ATPases were demonstrated by light and electron microscopy on the cytoplasmic membrane, findings similar to those in the stratum intermedium cells of the normal dental germ from which these tumor cells seem to arise. The tumor cells, like preameloblasts of the dental germ, also produce a granulo-filamentous material in intracytoplasmic vesicles and discharge it into the stroma. This “pseudo-amyloid” substance represents an abnormal protein of the enamel matrix and calcification, mainly occurring in that substance, might be an attempt at mineralization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689339
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  • 6
    Electronic Resource
    Electronic Resource
    Granular cell tumour of the mammary gland simulating malignancy (1984)
    Springer
    Virchows Archiv 403 (1984), S. 391-400 
    Details
    ISSN: 1432-2307
    Keywords: Immunohistochemistry ; Electron microscopy ; Light microscopy ; Granular cell tumour ; Mammary gland neoplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Primary granular cell tumours of the breast in 35 2and 55 year old women were studied by light microscopy, transmission electron microscopy and immunohistochemistry. Light and electron microscopy revealed a neural origin of the tumours and this was further substantiated by immunohistochemical studies, with positive S-100 protein reaction and negative reactions for surface heavy and light chains, CEA, alfa-1-antitrypsin, muramidase and GFA-protein. Granular cell tumour of the mammary gland is a very rare tumour. Clinically it sometimes simulates carcinoma because of its fibrous consistency, fixation to pectoral fascia and skin retraction. The diagnosis of granular cell tumour should be included in the differential diagnosis of carcinoma of the breast. The granular cell tumour is derived from neuro-ectodermal tissue. Whether it represents a neurogenic cell-confined metabolic disturbance with lysosomal activation, or a true neoplasm remains to be elucidated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00737288
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  • 7
    Electronic Resource
    Electronic Resource
    Electron microscopy of urinary calculi — Some facts and artefacts (1984)
    Springer
    Urological research 12 (1984), S. 17-22 
    Details
    ISSN: 1434-0879
    Keywords: Urinary calculi ; Electron microscopy ; X-ray microanalysis ; Artefacts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Material in urinary calculi is prone to irradiation damage during electron microscopy and this suggests the need for care in the interpretation of data. It is shown, however, that minimum-dose transmission microscopy is feasible for single-crystal electron diffraction work, and that although internal damage in severe, morphological artefacts are unlikely in the SEM unless the incident electron flux is greater than 10-13 A nm-2. During EDX micro-analysis, the detection of light elements is impaired by irradiation effects unless a minimum-dose procedure is used. For the preparation of SEM samples, artefacts can be created by cleaving air-dried material-and it is therefore important to consider more lengthy preparation methods such as cryogenics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256304
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  • 8
    Electronic Resource
    Electronic Resource
    Distribution of concanavalin A binding sites on normal human urinary bladder mucosa and bladder tumors by transmission and scanning electron microscopy and x-ray microanalysis (1984)
    Springer
    Urological research 12 (1984), S. 135-141 
    Details
    ISSN: 1434-0879
    Keywords: Concanavalin A ; Lectin receptors ; Bladder tumor ; Electron microscopy ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We used concanavalin A (con A)-peroxidase-iron dextran-diaminobenzidine (DAB) technique for the electron microscopic detection of con A binding sites on cell membranes. Normal bladder mucosa showed a sparse distribution of con A binding sites with both transmission (TEM) and scanning (SEM) electron microscopy, but bladder tumors showed a higher concentration in the distribution of con A binding sites in proportion to the histopathological grade of transitional cell carcinoma. Quantitative estimation of the con A binding sites was attempted using scanning X-ray pulse analysis of iron elements contained in the reaction complexes. Con A binding sites were quantitatively the smallest in normal mucosa, increasing proportionate to the grade of the bladder tumor. Some specimens were compared by the ferritin-labelled method and the pattern of ferritin conjugates distribution was similar to that seen with the con A-peroxidase-iron dextran method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257180
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  • 9
    Electronic Resource
    Electronic Resource
    A study of the ultrastructure of urinary calculi by scanning electron microscopy (1984)
    Springer
    Urological research 12 (1984), S. 227-230 
    Details
    ISSN: 1434-0879
    Keywords: Urinary calculi ; Stone ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study of urinary stones obtained from patients after surgery in the Medical College Hospital, Trivandrum, under the scanning electron microscope showed the presence of calcium oxalate and calcium biphosphate crystals as the main constituents. However, the pattern of the different phases of crystal growth was not uniform. Within the crystal lattice, fibrous structures, possibly of protein matrix, were invariably observed. Electron microscopy may be usefully adapted as a particularly suitable method for ultramicroscopic investigation of the fine structure of urinary stones including single crystal surface structure, section of urinary calculi and for possible presence of hitherto unknown components within the calculus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256809
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  • 10
    Electronic Resource
    Electronic Resource
    Peroxisomes in sebaceous glands (1984)
    Springer
    Anatomy and embryology 169 (1984), S. 9-20 
    Details
    ISSN: 1432-0568
    Keywords: Peroxisomes ; Marginal plates ; DAB-Cytochemistry ; Electron microscopy ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the canine circumanal gland, the morphological alterations of peroxisomes during differentiation and maturation of the glandular cells were studied by electron microscopy, cytochemistry and freeze-etch technique. Each of the following three cell types has its own characteristic peroxisomal population: 1) The basal cell contains only a few small peroxisomes, which appear as spherical and tubular profiles showing strong DAB reaction. In the differentiating basal cells, these are joined by a few dilated, hemispherical organelles with intensely stained small marginal plates. 2) In the intermediate cell, additional to spherical and tubular peroxisomes, numerous clongated organelles with distinct marginal plates are observed, displaying weak catalase activity. 3) In the mature cell, dumbbell-shaped peroxisomes with enlarged marginal plates predominate. Serial section analysis and freeze-etching studies reveal that these dilated particles are of erythrocyte-like shape. They exhibit very weak catalase activity or do not contain any visible DAB reaction product. In their flattened, thin central portions, the memoranes enclose the marginal plates and form straight cisternae, which are closely associated with adjacent fenestrated cisternae of ER on both sides, referred to as paramarginal cisternae. Dumbbell-shaped peroxisomes with their corresponding paramarginal cisternae form large peroxisome-ER-complexes. Furthermore, three to five dumbbell-shaped particles are often stacked in parallel. Only at their flat poles are the organelles in close contact with paramarginal cisternae. The observation of continuities, in particular between erythrocyte-like organelles and tubular peroxisomes in mature glandular cells, indicates the existence of a peroxisomal compartment composed of two segments in the mature stage. In freeze-etch replicas of mature glandular cells, only the dilated segments of the peroxisomal compartment can be easily recognized because of their unusual size and erythrocyte-like shape. Additionally, on the E-face of their central portion, a straight, square or rectangular area with a distinct crystalline pattern is seen, which corresponds to the marginal plate. These findings indicate that the circumanal gland of the dog offers unique possibility to analyze the biological properties of a well-defined peroxisomal compartment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300582
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  • 11
    Electronic Resource
    Electronic Resource
    Ultrastructure of the wall of the Dandy-Walker cyst (1984)
    Springer
    Acta neuropathologica 62 (1984), S. 225-229 
    Details
    ISSN: 1432-0533
    Keywords: Dandy-Walker syndrome ; Dandy-Walker cyst ; Ependymal cell ; Ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the wall of the Dandy-Walker cyst has been described rarely. A boy aged 2 years was confirmed clinically, neuroradiologically, and operatively as having a Dandy-Walker cyst in the posterior fossa. The cyst wall obtained during surgery consisted of an outer arachnoid cell layer, intermediate interwoven neuroglial strands, and an inner layer of cells which lacked the characteristic appearance of ependyma. An unusual finding was a small, buried island of ependymal cells in the intermediate layer of the neuroglial tissue. Ultrastructural study of the cyst wall provides a better understanding of the pathogenesis of the Dandy-Walker syndrome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691856
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  • 12
    Electronic Resource
    Electronic Resource
    Peripheral nerve findings in hereditary coproporphyria (1984)
    Springer
    Acta neuropathologica 63 (1984), S. 96-107 
    Details
    ISSN: 1432-0533
    Keywords: Hereditary coproporphyria ; Hereditary hepatic porphyrias ; Sural nerve biopsy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In spite of several cases reported in the literature, the exact pathogenetic mechanism of neuropathic changes in porphyric neuropathy remains uncertain. Various authors have ascribed the neuropathologic findings to either a dying-back axonal degeneration or segmental demyelination. In recent years, the hypothesis of an axonal and myelinic disorder has received support by the demonstration of a combined and simultaneous involvement of both these structures. Such different opinions are also a consequence of the reduced number of detailed bioptic observations in the different forms of acute porphyria not only during acute phases but also between attacks. In this paper we report the results of light- and electronmicroscopic examination of two sural nerve biopsies from subjects with hereditary coproporphyria. The first was performed 6 months after an acute attack, the second specimen was obtained from a patient without acute attacks, who had clinical and electrophysiologic signs of a chronic progressive neuropathy. In both cases a dying-back axonal degeneration is considered the primary change. The pathogenetic mechanism of peripheral nerve lesions in porphyric neuropathy will be discussed finally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697191
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  • 13
    Electronic Resource
    Electronic Resource
    Hemiplegic atrophy (1984)
    Springer
    Acta neuropathologica 62 (1984), S. 324-331 
    Details
    ISSN: 1432-0533
    Keywords: Human skeletal muscle ; Hemiplegia ; Cerebral vascular disease ; Histopathology ; Electron microscopy ; Rehabilitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Anterior tibial muscle biopsies of the hemiplegic side of 16 patients with a cerebrovascular accident in the middle cerebral artery region were analyzed qualitatively and quantitatively by enzyme histochemistry and electron microscopy. Patients grouped according to the time lapsed as from the occurrence of the accident (1–17 months) demonstrated a progressive decrease in the fiber diameter and changes in fiber type distribution with predominant type II atrophy and type I predominance. Nuclear internalization, myopathic alterations, and perifascicular fatty infiltrations were observed constantly. In the affected fibers the ultrastructural findings were myofibrillar alterations with the formation of rods and cytoplasmic bodies. There was accumulation of lipofuscin, glycogen, and lipid droplets. Microvascular changes were observed frequently. Biopsies from the asymptomatic legs were either normal or showed age-related muscle alterations. Correlation was noted between the clinical and functional status of the patients and the morphological aspects seen in muscle biopsies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687615
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  • 14
    Electronic Resource
    Electronic Resource
    Ultrastructure of carbon disulphide neuropathy (1984)
    Springer
    Acta neuropathologica 63 (1984), S. 255-263 
    Details
    ISSN: 1432-0533
    Keywords: Carbon disulphide ; Electron microscopy ; Giant axonopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Adult Wistar rats were exposed to carbon disulphide (CS2) vapour at a concentration of 2.4 mg/l of air for 5 days a week (6h a day), and the ultrastructure of peripheral nerves, neuromuscular junctions and muscles was investigated after 6 months of exposure to CS2. Numerous giant axons, i.e. paranodal or internodal swellings, were seen in the peripheral nerves. At the swollen paranodes, the myelin sheath was thinned, in other regions large intramyelinic vacuoles indicative of more dramatic demyelination were observed at axonal enlargements. Axonal enlargements consisted essentially of whorls of tightly packed neurofilaments. A number of nerve fibres underwent complete degeneration, but at the same time there was evidence of nerve regeneration. Nerve terminals were affected in a similar way following CS2 exposure. At neuromuscular junctions, filamentous swellings of nerve terminals preceded their degeneration and eventual denudation of synaptic gutters. As a rule, the postsynaptic part of neuromuscular junctions remained unimpaired by CS2 treatment. Muscles were affected by both atrophy and degeneration. Clusters of dense and lamellar bodies and numerous autophagosomes indicative of direct myotoxic effect of CS2 were frequently encountered in the investigated muscles. Some muscle fibres apparently underwent necrosis judging from the occurrence of myotubes characteristic of muscle degeneration and regeneration. The pathomorphology of CS2 neuropathy resembles that of other toxic neuropathies which presumably have a common origin in impaired energy metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685252
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  • 15
    Electronic Resource
    Electronic Resource
    Peroxisomes in sebaceous glands (1984)
    Springer
    Anatomy and embryology 169 (1984), S. 261-270 
    Details
    ISSN: 1432-0568
    Keywords: Peroxisomes ; DAB-cytochemistry ; Electron microscopy ; Three-dimensional reconstruction ; Preputial gland ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of peroxisomes in partially differentiated cells of the mouse preputial gland was investigated using serial thin sections and three-dimensional reconstruction as well as the alkaline diaminobenzidine technique for visualization of the peroxidatic activity of catalase. An analysis of serial sections indicates that the different types of intensely stained peroxisomal profiles, classified according to their shape, represent random planes through highly complex peroxisomes. These organelles exceed 4 μm in length and exhibit a focal heterogeneity with respect to their size, shape and enzyme distribution. The graphical three-dimensional reconstruction demonstrates that the most intricate peroxisomes are characterized by tortuous, elongate, and branched tubular segments of varying diameter equipped with enlarged terminal hollow-spherical structures which engulf areas of cytoplasm. A close spatial relationship is established between adjacent peroxisomes and peroxisomes and mitochondria, the latter two of which synchronously develop into highly complex structures. A close association is also observed between peroxisomes and the endoplasmic reticulum, whereby membrane continuities between the two compartments cannot be demonstrated. These observations are inconsistent with traditional concepts concerning peroxisomal shape and size, the number per cell, as well as their biogenesis from the endoplasmic reticulum. The functional significance of individual highly complex peroxisomes and their assemblage forming an extensive netlike membraneous system throughout the cell is discussed with respect to intracellular energy transport and trans-membrane electron exchange.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315631
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  • 16
    Electronic Resource
    Electronic Resource
    Ultrastructural studies of the temporal relationship between loss of zona pellucida and appearance of blastocyst-induced stromal changes during normal pregnancy in rats (1984)
    Springer
    Anatomy and embryology 170 (1984), S. 45-49 
    Details
    ISSN: 1432-0568
    Keywords: Implantation ; Blastocyst ; Zona pellucida ; Decidual reaction ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural studies were undertaken to investigate the temporal relationship between loss of the zona pellucida around the blastocyst and the appearance of decidual changes in the endometrial stroma during normal implantation in rats. Blastocyst-free and blastocyst-containing sites of pregnant uterine horns were studied and compared with control sites from contralateral salpingectomized horns and horns of pseudopregnant animals from 24.00 h on Day 4 and onwards. There were no membrane contacts between the blastocyst and the uterine epithelium at 10.00 h on Day 5 and earlier because of an intervening zona pellucida. From 14.00 h onwards, however, such contacts were present and at 18.00 h, the zona pellucida had disappeared and the blastocyst had attached onto the uterine epithelium. The stromal cells of pregnant and control horns were indistinguishable from each other at 24.00 h on Day 4, but from 06.00 h on Day 5 onwards specific changes were noted in the stromal cell nucleoli of the pregnant horns. The results therefore suggest that the first morphological sign of decidualization occurs about 12 h before the Pontamine Blue reaction and is initiated by the blastocyst early on Day 5 while it is still encased by the zona pellucida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319456
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  • 17
    Electronic Resource
    Electronic Resource
    An ultrastructural study on the “meconium corpuscles” in rat foetal intestinal epithelium with particular reference to apoptosis (1984)
    Springer
    Anatomy and embryology 169 (1984), S. 119-124 
    Details
    ISSN: 1432-0568
    Keywords: Meconium corpuscles ; Apoptosis ; Foetal intestine ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary During the embryonic development of the intestine, programmed deletion of epithelial cells plays a vital role in the formation of secondary lumina and of villi. Electron microscopy shows that this deletion is effected by apoptosis, a distinctive mode of cellular death recently recognised as being implicated in normal tissue involution in the adult, as well as during intrauterine development. The process involves cellular condensation and budding to form membrane-bounded apoptotic bodies containing well-preserved organelles. These are then either engulfed by neighbouring principal cells or sloughed off into the intestinal lumen. Engulfed apoptotic bodies that show various degrees of lysosomal degradation have previously been reforred to as “meconium corpuscles”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303140
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  • 18
    Electronic Resource
    Electronic Resource
    The use of semiautomatic morphometry in the study of normal rat gastrocnemius muscle fibers (1984)
    Springer
    Acta neuropathologica 64 (1984), S. 108-113 
    Details
    ISSN: 1432-0533
    Keywords: Image analysis ; Electron microscopy ; Muscle fibers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Based on a fixed number of EM photomicrographs a semiautomatic image analysis is made of normal gastrocnemius muscle fibers to obtain reference values for comparison with pathologic data. Z disc, mitochondria, tubular system, and sarcoplasmic reticulum are measured. It is stated that no definite fiber type differentiation is possible based on these quantitative data. However, measurements of Z disc and mitochondria are suggestive of such a differentiation. On the other hand, all data reach a Gaussian distribution on a logarithmic scale after a 5% correction factor is introduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695573
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  • 19
    Electronic Resource
    Electronic Resource
    A possible paracellular route for the resolution of hydrocephalic edema (1984)
    Springer
    Acta neuropathologica 64 (1984), S. 122-128 
    Details
    ISSN: 1432-0533
    Keywords: Hydrocephalus ; Blood-brain barrier ; Tight junctions ; Paracellular pathway ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Considering the possibility of a paracellular route for edema resolution we studied the microvasculature of the subependymal and subcortical white matter in hydrocephalic rats. Normal adult rats were used as controls. After injection of kaolin suspension into the cisterna magna, the animals were killed at intervals of 1, 2, 4, and 8 weeks. In hydrocephalic rats at 1 week after kaolin injection, widening of the interendothelical cleft between the tight junction (dehiscence) was seen in 27 of 76 (35%) vessels. At 2 weeks after kaolin injection, the number of the dehiscences had increased (39/7:56%) and some were enlarged, forming interendothelial blisters. At 4 weeks in hydrocephalic rats, both dehiscences and blisters were still prominent (45/73∶63%) and at 8 weeks the dehiscences were still prominent, but the number of the blisters had decreased (25/81∶31%). The blisters and dehiscences were most pronounced in the corpus callosum and occipital regions. Following i.v. injection of horseradish peroxidase, the interendothelial dehiscences and blisters were completely devoid of the marker substance. These findings indicate that in obstructive hydrocephalus the tight junctions may constitute part of a paracellular pathway for the resorption of interstitial edema fluid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695575
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  • 20
    Electronic Resource
    Electronic Resource
    A semiquantitative light and electron microscopic analysis of histopathologic changes in photochemotherapy-induced freckles (1984)
    Springer
    Archives of dermatological research 276 (1984), S. 2-11 
    Details
    ISSN: 1432-069X
    Keywords: Photochemotherapy ; Freckles ; Electron microscopy ; Melanocytes ; Langerhans cells ; Retinoid therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Photochemotherapy (PUVA)-induced freckles were found in 25 patients (41%) who had received more than 1,000 J/cm2 of PUVA. The patients had been treated with PUVA for more than 2 years, with more than 150 exposures before PUVA lentigines appeared on the thighs, the upper arm, the mid-lower arm, the waist, and the buttocks. The histopathology of these freckles was analyzed by light and electron microscopy. Light microscopy showed an increased amount of pigment and melanophages and increased numbers and size of melanocytes. The keratinocytes often displayed atypical features such as enlarged nuclei, giant size, or fibrillar degeneration. Homogenization of the papillary dermis was observed in 11 patients. The activation of melanocytes was confirmed electron microscopically, and pathological features such as large amounts of lipid droplets and lysosome-melanosome complexes within the melanocytes were seen. The Langerhans cells were mostly normal, whereas the keratinocytes showed cytolytic changes, fibrillar degeneration, and vacuolization. A close follow-up of patients with prolonged PUVA treatment is recommended.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412555
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  • 21
    Electronic Resource
    Electronic Resource
    Electron microscopic study on Langerhans cells and related cells in lymph nodes of DNCB-sensitive mice (1984)
    Springer
    Archives of dermatological research 277 (1984), S. 44-54 
    Details
    ISSN: 1432-069X
    Keywords: Birbeck granule ; Birbeck granule-like structure ; Electron microscopy ; Langerhans cell ; Lymph node
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To understand contact hypersensitivity, it is important to know the kinetics of Langerhans cells (LC) and related cells in the lymph node (LN), as well as in the skin. For this purpose, we tried experimentally to induce increased numbers of LCs, Birbeck granule-like structure (BgS)-containing cells, and interdigitating reticulum cells (IDC) in DNCB-sensitive mice and studied them by means of electron microscopy with the following results: (1) cytologically, LC, BgS-containing cells and IDC were closely related; (2) BgS seemed to arise from rough-surfaced endoplasmic reticulum (r-ER), and BgS-containing cells were midway in nature between LC and IDC from the morphological view point. From these findings, it appears that IDC, BgS-containing cells, and LCs were simultaneously involved in the contact hypersensitivity reactions of LNs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406480
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  • 22
    Electronic Resource
    Electronic Resource
    Hereditary motor and sensory neuropathy of demyelinating and remyelinating type in children (1984)
    Springer
    Acta neuropathologica 65 (1984), S. 1-9 
    Details
    ISSN: 1432-0533
    Keywords: Polyneuropathy ; Hereditary disease ; Chiloren ; Sural nerve biopsy ; Electron microscopy ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ten autosomal recessive/sporadic cases of hereditary motor and sensory neuropathy type I (HMSN I), nine of which originated from the northern part of Sweden, were included in the study. Parents were free from neurologic symptoms. Motor and sensory conduction velocity was normal when recorded, i.e., in 19 and 17 parents, respectively. Sural nerve biopsies from the ten cases revealed a varying degree of onion bulb formation. In eight of the cases the onion bulbs consisted of abundant basement membranes, whereas the Schwann cells were few and sometimes lacking. There were in some cases considerable differences between separate fascicles as to the loss of myelinated nerve fibers. In the six biopsies in which teasing was performed signs of present and previous demyelination were noticed. Numerous internodal segments were abnormally thin with reference to their length. In many such segments there were marked local thickenings of the nerve fiber. In cross sections the probable counterparts to these thickenings were nerve fibers with unduly thick myelin sheaths and complex folding of the myelin. Ultrastructural axonal changes were seen in the majority of the cases. The pathogenetic and diagnostic implications of the present findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689822
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  • 23
    Electronic Resource
    Electronic Resource
    Axonopathy and microangiopathy in chronic alloxan diabetes (1984)
    Springer
    Acta neuropathologica 65 (1984), S. 128-137 
    Details
    ISSN: 1432-0533
    Keywords: Quantitative ; Electron microscopy ; Microangiopathy ; Pi granules ; Schwann cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A significant reduction in the myelinated nerve fiber population was observed during quantitative electron-microscopic examination of peripheral nerves in chronic alloxan diabetic rats. Dystrophic axonal abnormalities and regenerating fibers were more numerous in diabetics than age-matched controls. Schwann cells showed reactive changes including prominent pi granules of Reich and intracytoplasmic filament accumulation. Enumeration of these alterations, however, revealed no singificant difference from controls. Endoneurial macrophages in diabetic rats were also filled with lamellar intracytoplasmic inclusions characteristic of a chronic neuropathy. Quantitation of pathologic lesions in teased nerve fibers confirmed the preponderance of axonal over demyelinative disease and showed demyelination to be segmental. Microangiopathy was noted throughout the vasa nervorum of diabetic rats, and quantitative electron microscopy showed endothelial proliferation with doubling of the number of endothelial cells and proportional capillary mural thickening. Swollen, reactive endothelial cells appeared to effece the vascular lumen and may impair capillary perfusion. These microcirculatory changes, in the presence of biochemical and rheologic disturbances may contribute to tissue hypoxia and underly the loss of axons in experimental diabetic neuropathy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690466
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  • 24
    Electronic Resource
    Electronic Resource
    Site of action of the natural algicide, cyanobacterin, in the blue-green alga, Synechococcus sp. (1984)
    Springer
    Archives of microbiology 138 (1984), S. 273-277 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Secondary metabolite ; Allelopathy ; Photosynthesis ; Electron transport ; Thylakoids ; Herbicides ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanobacterin is a secondary metabolite produced by the cyanobacterium, Scytonema hofmanni. Highly purified cyanobacterin was found to inhibit the growth of many cyanobacteria at a minimum effective dose of 2 μg/ml (4.6 μM). The antibiotic had no effect on eubacteria including the photosynthetic Rhodospirillum rubrum. The site of action of cyanobacterin was further investigated in the unicellular cyanobacterium, Synechococcus sp. Electron micrographs of antibiotic-treated Synechococcus cells indicated that cyanobacterin affects thylakoid membrane structure. The antibiotic also inhibited light-dependent oxygen evolution in Synechococcus cells and in spheroplasts. These data support our conclusion that cyanobacterin specifically inhibits photosynthetic electron transport. This activity is similar to herbicides such as 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU). The anhydro analog of cyanobacterin had no biological activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402134
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  • 25
    Electronic Resource
    Electronic Resource
    Structural analysis of EcoRI-DNA complexes as revealed by electron microscopy (1984)
    Springer
    Archives of microbiology 140 (1984), S. 265-270 
    Details
    ISSN: 1432-072X
    Keywords: EcoRI ; EcoRI-DNA complexes ; EcoRI* activity ; Recognition sites ; Frequency of binding ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electron microscopy of negatively stained isolated restriction enzyme EcoRI revealed particle projections with triangular or square outlines, indicating that the enzyme, in its tetrameric state, is tetrahedron-like. The two dimers making up the tetramer appear to be arranged in two planes orthogonal to each other. Complexes formed by EcoRI with the plasmids pBR322 or pGW10 were investigated by electron microscopic spreading techniques. In the presence of Mg2+, EcoRI was bound to the DNA molecules to form pearl necklace-like aggregates. The number of bound EcoRI particles was much higher as the sum of EcoRI-and 5′..AATT..3′ sites (with exceptions, the 5′..AATT..3′ sites may function as one type of EcoRI* sites) along the DNAs, indicating unspecific binding. In the absence of Mg2+, EcoRI was bound to the DNA only at the recognition site for EcoRI and the sites where the tetranucleotide sequence 5′..AATT..3′ was present. A direct correlation of the local concentrations of the bases A and T within the flanking sequences of the binding sites with the frequency of EcoRI to the DNA was observed. Dimers and tetramers of the enzyme was found to bind to the DNA. Tetramers occasionally exhibited two binding sites for DNA as indicated by the observation of DNA loops originating at the sites of bound tetrameric EcoRI particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454940
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  • 26
    Electronic Resource
    Electronic Resource
    Phospholipase activity in human tumors: Localization by cytochemical staining (1984)
    Springer
    Journal of cancer research and clinical oncology 107 (1984), S. 242-244 
    Details
    ISSN: 1432-1335
    Keywords: Phospholipase ; Plasma membrane ; Cytochemistry ; Electron microscopy ; Human tumors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Phospholipase activity was localized for the first time by cytochemical staining at the plasma membrane of malginant cells in gastric adenocarcinomas by electron microscopy. This activity was not found in benign cells of the same tumor, e.g., in components of the connective tissue underlying the glandular epithelium, or in cells beyond the margin of the same tumor. Neither benign nor maligant cells of colorectal tumors, nor those in malignant melanoma, had any phospholipase activity that could be demonstrated by the method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01032616
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  • 27
    Electronic Resource
    Electronic Resource
    Cytoplasmic inclusions and X-ray microprobe analysis in a case of prolymphocytic leukemia (1984)
    Springer
    Journal of cancer research and clinical oncology 107 (1984), S. 229-232 
    Details
    ISSN: 1432-1335
    Keywords: Prolymphocytic leukemia ; Cytoplasmic inclusions ; X-ray microanalysis ; Chronic lymphocytic leukemia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A patient with prolymphocytic leukemia is described. The peripheral blood and bone-marrow cells contained nuclear pockets, bridges, and appendices, as well as cytoplasmic inclusions that were not membrane bound or connected with the endoplasmic reticulum. X-ray microanalysis of the cells showed them to contain large amounts of phosphorus, sulfur, chlorine, and calcium, as well as a smaller amount of sodium and magnesium in comparison with control lymphocytes. When compared with lymphocytes of a patient with chronic lymphocytic leukemia (CLL), the patient's cells showed higher amounts of magnesium, sulfur, and chlorine, while the sodium content was decreased. The usefulness of electron microscopy and X-ray microanalysis in the diagnosis of this type of leukemia is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01032613
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  • 28
    Electronic Resource
    Electronic Resource
    Ellipsoids in the human spleen (1984)
    Springer
    Virchows Archiv 403 (1984), S. 27-40 
    Details
    ISSN: 1432-2307
    Keywords: Spleen ; Ellipsoids ; Morphometry ; Electron microscopy ; Lymphocyte traffic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study of ellipsoids (EL) in the human spleen was done on 25 surgically resected specimens in order to assess the number of EL, their structure, the nature of the cuff cells and the problem of lymphocyte traffic through their wall. The results show that an average spleen of 200 g contains 1.6 × 107 EL. They are localized in a constant segment of the vascular tree, just at the transition of the penicillary arterioles into capillaries and they consist of a capillary (not an arteriole) surrounded by a cuff of cells. These cells are neither endothelial nor smooth muscle cells, display lysosomal activity and are considered as fixed specialized pericapillary cells capable of cytoplasmic expansion. Since their number is inconstant in different individuals and in different species they are considered to be dynamic histological structures. Because of the constant presence of lymphocytes in their walls we consider the EL to be a transit system for lymphocytes and the splenic equivalent of the high endothelial venules in the lymph nodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689336
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  • 29
    Electronic Resource
    Electronic Resource
    Distribution of collagen types I and III and basal lamina in human gastric carcinoma: An immunohistochemical and electron microscopic study (1984)
    Springer
    Virchows Archiv 403 (1984), S. 313-322 
    Details
    ISSN: 1432-2307
    Keywords: Gastric carcinoma ; Scirrhous carcinoma ; Collagen ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Collagen types I and III were examined immunohistochemically in 32 cases of gastric carcinoma classified as poorly differentiated adenocarcinoma with scirrhous stroma, well differentiated adenocarcinoma with intermediate stroma, or poorly differentiated adenocarcinoma with medullary stroma. In the stroma of scirrhous carcinoma, types I and III collagens were distributed abundantly in fibrillar or granular patterns with little difference in the intensity of staining. In well differentiated adenocarcinoma, type I collagen was diffusely distributed in the stroma with type III collagen distributed sparsely. In poorly differentiated adenocarcinoma with medullary stroma, the two types of collagen were only found around capillaries, constituting the tumor interstitium. Electron microscopic examination of scirrhous carcinoma showed tumor cells partially covered with fibroblasts, and discontinuous basal lamina, collagen fibers and microfibrils present between tumor cells and fibroblasts. In well differentiated carcinoma, tumor cells were surrounded by fibroblasts, and well developed basal lamina was observed beneath the tumor cells. In poorly differentiated carcinoma with medullary stroma, the stroma consisted of capillaries and very few fibroblasts with discontinuous basal lamina occasionally being present between tumor cells and fibroblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00737282
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  • 30
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in the spermatogonia and spermatocytes of Poecilia reticulata during spermatogenesis (1984)
    Springer
    Cell & tissue research 237 (1984), S. 219-226 
    Details
    ISSN: 1432-0878
    Keywords: Spermatogonia ; Spermatocytes ; Carbohydrates ; Guppy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of guppy (Poecilia reticulata) spermatogonia and spermatocytes has been studied using electron microscopy. The spermatogonia, situated at the apex of the seminiferous tubule, are almost all surrounded by a network of Sertoli cells; they have very diffuse chromatin and one or two large nucleoli. The cytoplasm contains relatively few organelles, although annulate lamellae are found. The mitochondria have few cristae and are concentrated at one pole of the cell; they are sometimes found with intermitochondrial cement. These spermatogonia are separated from each other, having no intercellular bridges or inclusion in Sertoli cells, and are relatively undifferentiated; they correspond to stem cells. The spermatogonia beneath the apex are organized into cysts. First-generation spermatogonia are more dense and heterogeneous, their nuclei becoming smaller and their chromatin becoming denser during successive generations. In spermatocytes, the synaptinemal complex exists as a modified form until metaphase. The concentration of organelles in the cytoplasm increases and the organelles become more diversified as spermatogenesis progresses. Many cytoplasmic bridges are observed (several per cell), indicating that the cells remain in contact after several divisions. These changes in germ cell structure have been related to some of the characteristic features of spermatogenesis in guppy, e.g. the large number of spermatogonial generations and the complexity of spermiogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217139
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  • 31
    Electronic Resource
    Electronic Resource
    Marginal plates in hepatic peroxisomes of Ichthyophis glutinosus (Amphibia: Gymnophiona) (1984)
    Springer
    Cell & tissue research 238 (1984), S. 413-416 
    Details
    ISSN: 1432-0878
    Keywords: Peroxisomes ; DAB-cytochemistry ; Electron microscopy ; Liver, amphibian ; Gymnophiona ; Ichthyophis glutinosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of hepatic peroxisomes was investigated in Ichthyophis glutinosus (Amphibia: Gymnophiona), employing perfusion fixation and the diaminobenzidine (DAB) technique for the visualization of catalase. The majority of peroxisomes is circular or rod-shaped, although elongated particles occasionally occur. They contain a finely granular matrix, lightly stained after the DAB procedure. Their mean diameter is approximately 0.25 μm. Serial sections reveal that the circular and rod-shaped peroxisomal profiles are cross and oblique sections of highly tortuous, tubular organelles exceeding 2 μm in length. In addition to tubular profiles, elongated, rectangular particles, as well as straight dumbbell-shaped organelles with distinct marginal plates are observed. They range from 900 to 1650 nm in length (mean = 1200 nm). In the flattened, thin central portion of the dumbbell-shaped particle, the peroxisomal membranes form a cisterna enclosing one or two uniformly thick marginal plates, which display a definite substructure with a periodicity of 10 nm. These findings indicate that peroxisomes in the liver of Ichthyophis exhibit a complex organization. It is suggested that the organelles undergo a specific differentiation process, morphologically characterized by the formation of enlarged segments of unusual shape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217316
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  • 32
    Electronic Resource
    Electronic Resource
    Studies on the morphology of the isolated perfused rabbit ovary (1984)
    Springer
    Cell & tissue research 235 (1984), S. 59-63 
    Details
    ISSN: 1432-0878
    Keywords: Ovulation (rabbit) ; Graafian follicle ; Perfusion ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated ovaries from untreated, sexually mature rabbits were introduced into an in vitro perfusion system and perfused with a chemically defined medium containing albumin. The ovaries were perfused for up to 15 h (mean 11.5 h) and then processed for morphological investigation. Both at the light- and electron-microscopical levels, most of the ovaries exhibited a normal structure comparable with ovaries in situ. In two cases, however, marked accumulations of bacteria were found, although not inside the follicles. Since ovulation in the rabbit normally occurs between 9.5–13 h after mating or human chorionic gonadotrophin treatment, this model seems adequate for studies of ovulation in vitro. It is, however, important to study the ovaries microscopically after the perfusion to detect artifacts, e.g., bacterial infection, that may have influence on the process of ovulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213723
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  • 33
    Electronic Resource
    Electronic Resource
    Regions of putative acetylcholine receptors at synaptic contacts between neurons maintained in culture and subsequently fixed in solutions containing tannic acid (1984)
    Springer
    Cell & tissue research 235 (1984), S. 85-89 
    Details
    ISSN: 1432-0878
    Keywords: Tannic acid ; Acetylcholine receptors ; Tissue culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Spinal cord neurons from 9-day chick embryos were maintained in culture for up to 35 days and then fixed in 4% cacodylate-buffered glutaraldehyde containing 2% tannic acid. After about 15 days in culture a small percentage of the synaptic specializations present were characterized by striking electron-dense striations averaging 15 nm in width, oriented perpendicular to the postsynaptic membrane. These structures increased in frequency with time in culture (to a maximum of about 10% of all synapses in the oldest cultures); they were asymmetrical, protruding approximately 8 nm into the synaptic cleft, and more deeply (approximately 15–18 nm), into the postsynaptic cytoplasm. On the basis of earlier work by Sealock (1980) they are interpreted as concentrations of acetylcholine receptors. Similar membrane differentiations were also seen associated with active-zone areas of a few presynaptic membranes, and the possibility that these represent presynaptic acetylcholine receptors is discussed. Additional observations reported are (1) the presence of striations resembling those seen at the postsynaptic membrane in the membranes of some postsynaptic vesicles, and (2) filamentous links between the striations and cytoskeletal elements of the postsynaptic cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213727
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  • 34
    Electronic Resource
    Electronic Resource
    Fine structure of monoamine-containing basal cells in the taste buds on the barbels of three species of teleosts (1984)
    Springer
    Cell & tissue research 235 (1984), S. 479-484 
    Details
    ISSN: 1432-0878
    Keywords: Monoamine-containing cells ; Taste bud ; Paracrine cells ; Mechanoreceptors ; Electron microscopy ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The taste buds on the barbels in three species of teleosts (Cyprinus carpio, Misgurnus anguillicaudatus, Parasilurus asotus) were studied by means of fluorescence and electron microscopy. Intensely yellow-fluorescent cells, which are disk-shaped and located exclusively in a basal position, are observed in the barbel-buds of all fishes examined. The basal cells contain a large number of small clear vesicles approximately 40–60 nm in diameter, which show a tendency to aggregate in the cytoplasm facing the junction of the nerve terminals; chemically transmitting synapses are seen in the latter region. It is suggested from the present observations that the basal cells in the barbel-bud may originate from Schwann cells and have a dual function both as mechanoreceptors and paracrine elements. Since the administration of 5,6-DHT results in an appearance of small dense vesicles among the small clear vesicles, the possibility exists that the basal cell may be capable of taking up monoamines and storing them in the small clear vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226942
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  • 35
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    Electronic Resource
    Electron-microscopic demonstration of the distribution of calcium deposits in the exocrine pancreas of the rat after application of carbachol, atropine, cholecystokinin, and procaine (1984)
    Springer
    Cell & tissue research 235 (1984), S. 683-690 
    Details
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Calcium pool ; Calcium release ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In an attempt to identify a cellular Ca2+-pool, from which calcium is released when secretagogues are applied, tissue fragments of the rat exocrine pancreas were incubated and fixed with glutaraldehyde in the presence of calcium. By means of this procedure electron-dense deposits were found on plasma membranes. X-ray microanalysis showed that these deposits contain calcium. Stimulation of tissue fragments with the use of the secretagogues carbachol or cholecystokinin reduced the number of deposits by about 80%. When the antagonist atropine was applied after carbachol stimulation, deposits reappeared on cell membranes, which then disappeared again after a second stimulation with cholecystokinin. In the presence of procaine, carbachol was inhibited and only slightly reduced the Ca2+-deposits on the plasma membranes. These results suggest that a calcium pool, from which calcium is released to induce enzyme secretion on stimulation, is located in the cell membrane
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    URL: http://dx.doi.org/10.1007/BF00226969
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  • 36
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    Electronic Resource
    Morphological and morphometrical changes in chloride cells of the gills of Pimephales promelas after chronic exposure to acid water (1984)
    Springer
    Cell & tissue research 236 (1984), S. 121-128 
    Details
    ISSN: 1432-0878
    Keywords: Chloride cells ; Acid stress ; Gill ; Electron microscopy ; Fathead minnow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fathead minnows, Pimephales promelas, were exposed for 129 days to Lake Superior water acidified with sulfuric acid by means of a flow-through toxicant injection system. The effects of chronic acid stress (pH 6.5, 6.0, 5.5, 5.0) on gill histology were examined. Most of the histological effects were seen at pH 5.5 and 5.0 and were confined primarily to changes in numbers, distribution, and morphology of chloride cells. At low pH levels there tend to be more chloride cells in the gill epithelium and an increased percentage of these cells in the secondary lamellae. In contrast to normal chloride cells, chloride cells from fish exposed to low pH frequently had apical pits while some had bulbous apical evaginations. The occurrence of structural changes in chloride cells during exposure to acid water suggests that chloride cells may be involved in acclimation to acid stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216521
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  • 37
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    Electronic Resource
    Morphometric evaluation of lipid droplet associations with secretory vesicles, mitochondria and other components in the lactating cell (1984)
    Springer
    Cell & tissue research 236 (1984), S. 471-475 
    Details
    ISSN: 1432-0878
    Keywords: Lactating cell ; Lipid droplets ; Secretory vesicles ; Mitochondria ; Intracellular associations ; Electron microscopy ; Milk secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The size, cellular location, and identity of surface-associated components were determined for lipid droplets in lactating cells. Transmission electron-microscopic measurements were made involving 3801 droplets in approximately 211 cells from three rats and 1197 droplets in 66 cells from a mouse. For the purposes of droplet evaluation, cells were divided into seven locations ranging from basal to secreting positions. Droplets were also categorized with respect to contact with other droplets, basolateral plasma membrane, mitochondria, Golgi apparatus, secretory vesicles, and endoplasmic reticulum-cytoplasm (ERC). Data on droplet size showed that droplet growth occurs mainly in the secretory position, confirming previously published findings. Lipid droplets from mouse tissue, although somewhat smaller in size showed similar growth trends to those of the rat. Data on numbers of droplet contacts and percentages of droplet circumferences involved in associations with other cell components showed that the dominant interaction of lipid droplets was with the ERC. However, intimate association of droplets with mitochondria was noted in all cellular locations. In addition, nursed animals exhibited a greater proportion of droplet surface association with secretory vesicles and less in contact with mitochondria in comparison to those not nursed. The significance of these relationships to milk synthesis and secretion is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214252
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  • 38
    Electronic Resource
    Electronic Resource
    Electron-microscopic study of silver staining of nucleoli in growing oocytes of rat ovaries (1984)
    Springer
    Cell & tissue research 236 (1984), S. 249-255 
    Details
    ISSN: 1432-0878
    Keywords: Oocyte ; Nucleolus ; Silver staining ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nucleoli of dictyate-stage growing oocytes in rat ovaries were examined both with routine electron microscopy and electron microscopy after silver nitrate and ammoniacal silver nitrate (Ag-AS) staining. The nucleoli of the unilaminar follicular oocytes consist of twisted strands of dense fibrillar components, aggregates of granular components, and small fibrillar centers. After Ag-AS staining, silver grains are numerous on the dense fibrillar strands, fewer on the fibrillar centers, and very sporadic on the granular aggregates. The same stainability of three nucleolar components with the Ag-AS method was also confirmed in the nucleoli segregated by actinomycin D. During the transition of growing oocytes from bilaminar to plurilaminar follicle stage, the nucleolar dense fibrillar strands gradually conglomerate and are transformed into large and compact spherules. The stainability of dense fibrillar components with the Ag-AS method was lost along with this nucleolar transformation. These results may provide some new clues on the functional significance of AgAS-positive proteins in the nucleoli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214225
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  • 39
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    Electronic Resource
    Structure of the parathyroid glands, as revealed by different methods of fixation (1984)
    Springer
    Cell & tissue research 235 (1984), S. 51-58 
    Details
    ISSN: 1432-0878
    Keywords: Parathyroid glands ; Electron microscopy ; Light microscopy ; Quantitative histology ; Mongolian gerbil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Stereology and semi-automatic image analysis were used with the aim of comparing the structure of parathyroid glands from untreated adult Mongolian gerbils fixed by immersion with those fixed by perfusion. Subclassification of the chief cells based upon the staining affinity or electron density of the cytoplasm was readily performed only in glands fixed by immersion, and so-called atrophic cells were observed only in these glands. The atrophic cells were often surrounded by “light” chief cells. In glands fixed by perfusion, “light” chief cells were only rarely encountered. A significant difference between glands fixed by immersion and those fixed by perfusion was found only with regard to the form of cells and nuclei, those fixed by perfusion being more spherical. When comparing individual cells within glands fixed by immersion, “light” chief cells were more spherical and had a significantly larger nuclear and cellular size, and a lower mitochondrial volume density than the “intermediate”/“dark” chief cells. Otherwise there were no significant differences in any of the parameters investigated. These data indicate that occurrence of socalled “light” chief cells and atrophic cells is a result of improper fixation. The results of this study do not favour the concept of a functional cycle with a simultaneous occurrence of active and inactive cells within parathyroid glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213722
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  • 40
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    Electronic Resource
    Interdigitating cells in the lymphoid tissues of bovine fetuses and calves (1984)
    Springer
    Cell & tissue research 235 (1984), S. 153-158 
    Details
    ISSN: 1432-0878
    Keywords: Bovine ; Interdigitating cell ; Lymphoid tissues ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron-microscopic studies of lymphoid tissues from bovine fetuses and from calves disclosed a non-lymphoid cell type in the thymus-dependent zones of secondary lymphoid tissues and in the thymus that is distinguishable from reticulum cells, epithelial and endothelial cells, and macrophages. Based on morphological and topographical criteria, the cell is identified as the interdigitating cell. In addition, studies of the tissues of normal and virus-challenged fetuses, and of conventionally reared calves, indicated that the interdigitating cells originate from monocytoid cells, which undergo differentiation in the thymus-dependent zones during an immune response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213735
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  • 41
    Electronic Resource
    Electronic Resource
    The fine structure of identified electrotonic synapses following increased coupling resistance (1984)
    Springer
    Cell & tissue research 235 (1984), S. 243-249 
    Details
    ISSN: 1432-0878
    Keywords: Gap junction ; Electron microscopy ; Freeze fracture ; Cell-to-cell communication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparations were used to monitor the morphological changes which occurred up to 45 min after injury. There was no apparent change in the organization (a loose polygonal array) of the intramembrane particles which make up the junctional complex up to 45 min after injury. In some instances, however, the intramembrane particles appeared to have moved away from the junctional area. Other junctional regions were internalized and appeared similar to what have been called annular gap junctions. Also at this time (20–25 min after injury), a dense cytoplasmic plug formed in uninjured axon near the junctional region. It is concluded that the gap junctions that exhibit a loose polygonal organization of the intramembrane particles may be either in a state of low resistance (coupled) or a state of high resistance (uncoupled).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217847
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  • 42
    Electronic Resource
    Electronic Resource
    Studies on the morphology of the isolated perfused rabbit ovary (1984)
    Springer
    Cell & tissue research 235 (1984), S. 565-573 
    Details
    ISSN: 1432-0878
    Keywords: Ovulation ; Perfusion ; Graafian follicle (rabbit) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ovulation was induced in rabbits by intravenous administration of human chorionic gonadotrophin (HCG), and 4–5 h later the ovaries were isolated and introduced into an in-vitro perfusion system containing synthetic medium with albumin. Rupture of follicles occurred in vitro within the physiological time range (mean 11.3 h after injection of HCG), although with a reduced frequency. Preovulatory and ruptured follicles were studied in detail by light and electron microscopy. In the granulosa layer of ruptured or preovulatory follicles cytoplasmic blebbing activity, disappearance of CallExner bodies and differentiation toward luteinized cells were found. Perhaps the most important sign of normal preovulatory development in vitro was that the basement membrane surrounding the granulosa layer was penetrated by projections of granulosa cells. In the absence of this penetration phenomenon the granulosa layer prolapsed out of the follicle. Immediately before rupture, follicles showed marked degeneration, restricted to the outer layers of the apical wall, which is compatible with the hypothesis that degradative enzymes are released close to the surface of preovulatory follicles. Although the majority of follicles that ovulated under in-vitro conditions showed the same kind of morphological alterations as can be seen in vivo, occasional atypical ruptures occurred without any overt signs during perfusion. Also technical manipulations of the perfusion system, e.g., nonphysiological increase of perfusion pressure, could force follicles to rupture. This illustrates the importance of careful morphological study of all ovaries perfused in vitro before conclusions are drawn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226954
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  • 43
    Electronic Resource
    Electronic Resource
    Electron-microscopic investigation of the innervation of the pars distalis of the hypophysis in adult Rana temporaria L. (1984)
    Springer
    Cell & tissue research 237 (1984), S. 149-154 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland, pars distalis ; Innervation ; Synaptoid contacts ; Electron microscopy ; Rana temporaria L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the pars distalis of the hypophysis of adult Rana temporaria, three types of nerve-fiber profiles were found at two distinct sites, in both lateral parts of the bordering regions of the anterior lobe with the intermediate lobe of the hypophysis. The first type of nerve-fiber profile consists of bundles of very fine axonal elements (diameter: 〈0.7 μm). The second type is formed by larger nerve fibers (diameter up to 4 μm) containing a few neurosecretory granules of approximately 100 nm. The third type of nervefiber profile resembles the second type but these nerve fibers make synaptoid contacts on at least two different types of glandular cells. The possible functional significance of these nerve fibers in the pars distalis is discussed. No nerve fibers were found (1) in the central part of the bordering region of the pars distalis with the intermediate lobe, (2) at the bordering region with the median eminence and (3) with the neurohypophysial stalk, and (4) in all other parts of the pars distalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229210
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  • 44
    Electronic Resource
    Electronic Resource
    Vascularization and glomerular ultrastructure in the pig mesonephros (1984)
    Springer
    Cell & tissue research 238 (1984), S. 165-175 
    Details
    ISSN: 1432-0878
    Keywords: Mesonephros ; Pig embryo ; Glomerulus ; Microvasculature ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vascularization of the pig mesonephros was investigated in embryos 5–8 cm in length. Vascular injections with microfil were cleared and dissected; corrosion casts were studied under the scanning electron microscope (SEM). Perfusion-fixed tissue was used for SEM and transmission electron microscope (TEM) studies, including freeze-fracture specimens. The branches of one mesonephric artery carry up to 15 glomeruli. Several glomeruli occupy the same arterial branch, with very short afferent arterioles proper. The efferent vessels, frequently 2–5, leave the extensive vascular pole opposite the entering arteriole and split into peritubular capillaries radiating towards the superficial veins. These capillaries form vascular regions in the shape of flattened pyramids. Along its course, one nephron is supplied by vessels derived from 4–7 glomeruli. The nephrons have less vascular contact than in the definitive kidney. The ultrastructure of the single mesonephric vessels matches the metanephric counterparts. Epithelioid cells with renin granules are common in afferent arterioles, larger arteries, and efferent vessels. The lobulated glomeruli are up to 750 μm long and flattened, showing the usual features of podocytes, mesangial cells, and an attenuated endothelium with fenestrations between 50 and 250 μm. It partially retains its own basement membrane. There is no proximal mesangium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215158
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  • 45
    Electronic Resource
    Electronic Resource
    The effect of long-term denervation on the ultrastructure of Pacinian corpuscles in the cat (1984)
    Springer
    Cell & tissue research 238 (1984), S. 387-394 
    Details
    ISSN: 1432-0878
    Keywords: Pacinian corpuscles, cat ; Denervation ; Atrophy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of Pacinian corpuscles of the cat located in the crural region and innervated by the interosseous nerve was studied 1 to 14 months after denervation. Both the Pacinian inner core and capsule remained well preserved one month after denervation. However, the denervated inner cores underwent progressive atrophy and wasting, which resulted in a gradual reduction of the amount of inner-core cells and lamellae, widening of interlamellar clefts, formation of empty spaces in the axial region and a considerable increase in the number of collagen fibrils. In spite of the wasting, the inner core still survived 14 months after denervation, but at least half of its volume became occupied by collagen fibrils which surrounded the remaining inner-core cells and lamellae. Collagen fibrils assembled in the denervated core were markedly thinner than those found in the capsule, as is also the case in normal Pacinian corpuscles. In the capsule, discrete focal degeneration, occasional pyknosis of the innermost capsular cells and macrophage infiltration were observed from the first month after nerve section onward, but the number of capsular layers remained within the normal range (30–40) up to 14 months after denervation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217312
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  • 46
    Electronic Resource
    Electronic Resource
    Enhanced cellular membrane contrast in a marine alga by Osmium-azole complexes (1984)
    Springer
    Protoplasma 119 (1984), S. 48-54 
    Details
    ISSN: 1615-6102
    Keywords: Electron microscopy ; Marine alga ; Membrane contrast ; Osmium-azole complexes ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Addition of certain heterocyclic nitrogen-carbon compounds to standard osmium tetroxide solutions used as secondary fixative resulted in an enhanced general membrane contrast in cells of the marine algaEmiliania huxleyi (Lohmann) Kamptner. Ultrastructural cell morphology and the contrast distribution were compared between cells treated according to a standard secondary fixation procedure and cells post-fixed when above mentioned heterocyclic compounds were introduced; in both cases some of the ultrathin sections were post-stained. Different compounds were tested: 1,2,4-triazole (TRA), 3-amino-1,2,4-triazole (A-TRA), 5-amino-tetrazole (A-TEA) and 2,4,6-tri-amino-1,3,5-triazine (melamine). The results were interpreted to indicate the possible bonding types arising from interaction of the heterocyclic compounds with osmium tetroxide and with membrane constituents. Interpretations were partly inspired by considerations from coordination chemistry. All above tests which did not include post-staining of thin sections could be performed at alkaline pH, and consequently calcified structures were preserved. The enhanced osmium accumulation at membranes was verified with X-ray microanalysis, which also showed that in the cases where membranes were visibly contrasted, localization of probable sites of intracellular non-crystalline calcium was facilitated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287816
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  • 47
    Electronic Resource
    Electronic Resource
    Biological and biochemical analysis of soils (1984)
    Springer
    Plant and soil 76 (1984), S. 127-137 
    Details
    ISSN: 1573-5036
    Keywords: Adenylate pool ; Biomass volume ; CO2 evolution ; Chitin ; DNA ; Electron microscopy ; Enzymes ; Fluorescent antibody ; Fumigation-respiration ; Fungi Histochemistry ; Imunofluorecence ; Jones-Mollison technique ; Microcosms ; Monoclonal antibodies ; Nitrogen ; Nutrients ; Oxygen consumption ; Phosphorus ; Phytotoxins ; Plate counts ; Rhizobium ; Rhizosphere ; Sulphur ; Xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary There is an immense literature on biological and biochemical analyses of soils. Such analyses have revealed the enormous richness of species in soil and their vast range of metabolic potentials and ecological diversity. Accordingly, the approaches used to investigate the soil biota and its biochemistry usually have to be modified or adapted depending upon the purpose of the investigation. Studies of micro-organisms in the soil environment, are complicated because microbial cells are commonly attached to surfaces where they live side-by-side with other populations in consortia usually containing different morphological and physiological types. Such assemblages of organisms cannot be described quantitatively using cultural techniques, such as plate counts, which underestimate both cell numbers and viable biomass. The development of more powerful observational and staining techniques has improved our knowledge of the diverse morphological and biochemical composition of soil micro-communities. Such findings have been amplified at a grosser level by laboratory studies with multi-component systems (microcosms) to mimic field situations and to assess the range of biochemical potentials of microbial consortia. But despite notable advances in analytical methods we are still, with a few exceptions, unable to detect or identify those microorganisms which carry out specific biochemical transformations or determine whether particular cells are alive, dormant or dead at the time of observation. Considerable work has been done to define some of the fundamental ecological attributes of microbial assemblages in soil. Productive work on the metabolic activities of the soil microbiota, specially geochemical transformations of C, N, S and P, has been under way for more than a century. But only in more recent years have more sensitive and reproducible analytical methods become available to measure viable biomass in soil. This will enable some insight to be gained into the role that microbial biomass plays as a labile source and sink for plant nutrients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02205573
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  • 48
    Electronic Resource
    Electronic Resource
    Soil organic matter and structural stability: mechanisms and implications for management (1984)
    Springer
    Plant and soil 76 (1984), S. 319-337 
    Details
    ISSN: 1573-5036
    Keywords: Aggregates ; Aluminium ; Bacterial mucilage ; Binding agents ; Calcium ; Cation bridges ; Complexing agents ; Dispersion ; Electron microscopy ; Electrophoretic mobility ; Fungal hyphae ; Glues Iron ; Management Periodate ; Polysaccharides ; Rhizosphere ; Roots ; Slaking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The stability of pores and particles is essential for optimum growth of plants. Two categories of aggregates macro- (〉 250 μm) and micro- (〈250 μm) depend on organic matter for stability against disruptive forces caused by rapid wetting. Dispersion of clay particles from microaggregates is promoted by adsorption of complexing organic acids which increase the negative charge on clays. The acids are produced by plants, bacteria and fungi. However, the dispersibility of clay in microaggregates is offset by the binding action of polysaccharides, mainly mucilages produced by bacteria, but also by plant roots and fungal hyphae. The stability of microaggregates is also enhanced by multivalent cations which act as bridges between organic colloids and clays. Macroaggregates are enmeshed by plant roots, both living and decomposing, and are thus sensitive to management, and increase in number when grasses are grown and the soil is not disturbed. Lack of root growth,i.e. fallow, has the opposite effect. Various implications for management of soil structure are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02205590
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  • 49
    Electronic Resource
    Electronic Resource
    Cytochemical localization of adenylcyclase in the lateral wall of the inner ear (1984)
    Springer
    European archives of oto-rhino-laryngology and head & neck 240 (1984), S. 55-61 
    Details
    ISSN: 1434-4726
    Keywords: Inner ear ; Adenylcyclase ; Ouabain-insensitive endothelial ATPase ; Cytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cytochemical localization of adenylcyclase in the lateral cochlear wall was studied using a modification of the Reik-Howell method. All the cell membranes of the stria vascularis and spiral prominence cells, except the vascular endothelium, showed a low enzyme activity. In the marginal cell and spiral prominence epithelium facing the endolymph, this activity was limited to the perilymphatic membrane sections. The results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00464345
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  • 50
    Electronic Resource
    Electronic Resource
    High resolution gas chromatography: Simultaneous detection of narcotics, adulterants, and diluents in street samples (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 140-141 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Narcotics: street samples ; Narcotics, adulterants and diluents: simultaneous determination ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070308
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  • 51
    Electronic Resource
    Electronic Resource
    Enantiomer separation of some aromatic carboxylic acids by HPLC on a chemically bonded β-cyclodextrin stationary phase (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 147-148 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; HPLC ; Racemate separation ; β-Cyclodextrin stationary phase ; Cyclohexylphenylglycolic acid ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070311
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  • 52
    Electronic Resource
    Electronic Resource
    A simple effluent splitter for capillary gas chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 154-155 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Effluent splitter ; Dual detection ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070315
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  • 53
    Electronic Resource
    Electronic Resource
    Chromatography calendar (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 165-166 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070320
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  • 54
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    Electronic Resource
    HPLC on 3,5-dinitrobenzamidopropyl silica gel (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 160-161 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography HPLC ; Bonded phases ; 3,5-Dinitrobenzamidopropyl silica gel ; Charge transfer interaction ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070318
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  • 55
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    Electronic Resource
    High performance thin layer chromatography - densitometric method for content uniformity test of lanatoside C tablets (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 161-164 
    Details
    ISSN: 0935-6304
    Keywords: High-performance-thin layer chromatography, HPTLC ; Zirconium oxychloride ; Backside fluorodensitometric detection on TLC plate ; Content uniformity test for lanatoside C tablets ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070319
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  • 56
    Electronic Resource
    Electronic Resource
    Developments in pyrolysis capillary GC (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 172-184 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070402
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  • 57
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    Electronic Resource
    Multilayer effects in liquid adsorption chromatography with mixed mobile phases (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 203-207 
    Details
    ISSN: 0935-6304
    Keywords: Liquid adsorption chromatography, LSC, HPLC ; Multilayer effects in LSC ; HPLC with mixed mobile phase ; HPLC theory ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper describes the theory of liquid adsorption chromatography (LSC) with mixed mobile phase, involving formation of multilayer surface phases. An equation describing the dependence of the capacity ratio upon the mobile phase composition is derived and theoretical curves illustrating the same dependence are calculated accordingly by assuming different thicknesses of the surface phase.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070406
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  • 58
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    Electronic Resource
    Separation of distearoylphosphatidylcholine, dipalmitoylphosphatidylcholine, and dioleylphosphatidylcholine by thin-layer chromatography using new modified plates (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 211-212 
    Details
    ISSN: 0935-6304
    Keywords: Thin layer chromatography, TLC ; Alkylchlorosilanes ; Phosphatidylcholines ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070408
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  • 59
    Electronic Resource
    Electronic Resource
    Masthead (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984) 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070501
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  • 60
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    Electronic Resource
    Reversed-phase separation of tetodotoxin and its oxidized derivative at the C11-position detected spectrophotometrically at 195 nm (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 265-267 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; C18-silica ; UV-detection ; Tetrodotoxin ; Oxidized derivative ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070506
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  • 61
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    Electronic Resource
    High performance liquid chromatography determination of prochloraz residues in citrus fruit (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 268-270 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Citrus fruit ; Fungicide residues ; Prochioraz ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070507
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  • 62
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    Electronic Resource
    High resolution gas chromatography (HRGC) of the lipids extracted from blood serum (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 274-276 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, glass ; Liquid-liquid and liquid-solid extraction ; Serum lipids ; Fatty acids ; Cholesterol and cholesteryl esters ; Triglycerides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070509
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  • 63
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    Electronic Resource
    Picomole amounts of methyl chloride by reaction gas chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 280-281 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Reaction GC ; Iodination reactor ; Electron capture detection ; Trace analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070512
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  • 64
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    Electronic Resource
    Chromatography calendar (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 289-291 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070516
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  • 65
    Electronic Resource
    Electronic Resource
    The optimized separation of aromatic carboxylic acids by HPLC (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 375-381 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Reverse phase columns ; Window diagrams ; Aromatic carboxylic acids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The optimization of the separation of eight aromatic carboxylic acids, using Hypersil SAS and MOS reverse phase columns, with respect to eluent (methanol : water) composition, pH, and temperature, is described. Window diagram techniques are used and the pH of the aqueous buffer and elution temperature are shown to be more critical than eluent composition.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070707
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  • 66
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    Enantiomeric separation of halocarboxylic acids by capillary gas chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 398-403 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Enantiomeric separation ; Racemization ; Halocarboxylic acids ; Amide derivatives ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The direct determination of the enantiomeric purity of halocarboxylic acids is described. The method used involves gas chromatographic separation of the corresponding tert-butylamides on deactivated glass or fused-silica capillaries coated with Chirasil-Val. Both the influence of the amide moiety on enantiomeric resolution and tailing, and the racemization during formation of the amide derivatives were studied.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/jhrc.1240070710
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    Device for injection of absorbent-trapped compounds on to a WCOT column (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 477-479 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Glass capillary column ; Adsorbent ; Cold trapping ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070806
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  • 68
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    Analysis of pesticide residues by high resolution gas chromatography. Part 1: Comparison between packed and capillary columns in pesticide residue determinations. Practical considerations for routine use of capillary gas chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 480-484 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Packed and capillary columns ; Fungicides and insecticides ; Adsorption ; Length of column ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070807
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  • 69
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    Sensitization of thiamine analysis by the peak compression technique (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 490-491 
    Details
    ISSN: 0935-6304
    Keywords: Column liquid chromatography, HPLC ; Peak compression technique ; Precolumn derivatization ; Thiamine compounds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070810
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  • 70
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    Product news (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 499-500 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070814
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  • 71
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    Masthead (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984) 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070901
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  • 72
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    Characterization of OV-1/FFAP-mixture coated glass capillary columns used for the separation of free fatty acids (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 509-514 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; OV-1/FFAP mixed phase, high-temperature ; silylated glass capillary ; Polar phase-coated HTS capillary ; Golay curves of mixed phases ; Thermal and long-term stability of OV-1/FFAP ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: As an alternative to acid pretreated UCON and FFAP capillaries for the analysis of wide-boiling free fatty acid mixtures, OV-1 : FFAP phase mixtures were used on high-temperature silylated inert glass capillary columns. The HETP-carrier gas velocity curves, peak asymmetry factors, coating efficiency, gas phase and stationary phase contributions to the mass transfer resistance were determined for various OV-1 : FFAP ratios. Mixed-phase capillaries showed optimum performance at a 2 : 1 OV-1 : FFAP ratio. The thermal and long-term stability of OV-1-stabilized FFAP columns surpassed those of the UCON and pure FFAP reference column used.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070903
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  • 73
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    The length of the zone flooded by the injection of large volumes onto retention gaps in capillary GC (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 531-532 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; Capillary columns ; Retention gaps ; Large sample volumes ; Flooded zone ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070908
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  • 74
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    High speed zone electrophoresis in open-tubular fused silica capillaries (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 529-531 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrophoresis ; High resolution separation ; High speed analysis ; Dansyl amino acids ; Fluorescamine labeled peptides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070907
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  • 75
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    Advantages and limits of the quantitative determination of α-ferrocenyl carbinols with capillary GC (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 536-537 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column ; On-Column injection ; Vaporizing injection ; Decomposition ; α-Ferrocenyl carbinols ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070910
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  • 76
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    The Analysis of volatile compounds by purge and trap with whole column cryotrapping (WCC) on a fused silica capillary column (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 504-508 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Fused silica capillary columns ; GC/MS/DS ; Cryotrapping and cryofocussing of organics ; Purgeable compound analysis ; Trace organic analysis ; Priority pollutants ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The purge and trap (P&T) method of analysis has been interfaced with fused silica capillary column gas chromatography. This interfacing has been accomplished without splitting the P&T trap desorption carrier gas. Thus, 100% of the purged compounds are transferred to the column. The analytes are cryofocussed on the column using whole column cryotrapping (WCC) at -80°C. The resulting P&T/WCC procedure is extremely well-suited to the analysis of trace purgeable aqueous organic compounds. Samples and standards containing a variety of aromatic standard compounds were analyzed. The standards included benzene, toluene, ethylbenzene, xylenes, C3-C4-benzenes, and naphthalene, as well as three P&T internal standard compounds. Chromatographic peak widths were uniformly less than 6 s at the base and excellent precision was obtained in the relative retention time data for all compounds. The chromatogram of a groundwater sample contaminated with aromatic gasoline compounds is also presented. Since P&T/WCC works well with fused silica capillary columns, the full sensitivity and chromatographic efficiency of capillary gas chromatography is made available to P&T analyses.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/jhrc.1240070902
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  • 77
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    Optimization of retention time of aromatic acids in liquid chromatography from log P and predicted pKa valuesThis paper was presented at VIIth International Symposium on Column Liquid Chromatography, Baden-Baden, May (1983) (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 524-528 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Reversed phase mode ; Prediction of retention time ; Prediction of pKa ; Hydrophobic fragmental constant ; Aromatic acids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: For optimization of liquid chromatography separations, several approaches have been reported, among them the use of the hydrophobic fragmental constant, as proposed by Rekker [1]. The fragmental constant is derived from the partition coefficients of various solutes between octanol and water and represents a summation of solubility parameters: van der Waals volume, π - π interactions, hydrogen bonding, etc. With these constants the optimization of reversed phase mode liquid chromatography using octadecyl bonded silica gels as packing was feasible, although large error was found for the eluent with a high concentration of organic modifier or water. This is probably due to the heterogeneous solvation of the surface of the packings and to various solvent effects. In addition, when the acid dissociation constant was known, the retention times of aromatic acids in the ionic form were also predicted using the hydrophobic fragmental constant and the dissociation constant. By comparison of the previous data and a method proposed by Perrin et al. [2] it was possible to predict the dissociation constant of aromatic acids and optimize separation in reversed phase mode liquid chromatography using octadecyl bonded silica gels as packing. Furthermore, an equation for calculating the capacity ratios in a given pH eluent was proposed and the predicted and observed capacity ratios of aromatic acids in several eluents were examined in order to improve the above system.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070906
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  • 78
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    Kovats retention indices of selected olefins on bonded phase fused silica capillaries (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 542-544 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Fused silica (capillary) columns ; Bonded (cross-linked) phases ; Hydrocarbon analysis ; Olefinic hydrocarbons ; Retention index data ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070913
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  • 79
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    Analysis of mutagenic amino acid pyrolyzates with a fused silica capillary column (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 563-565 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; Fused silica capillary columns ; Amino acid pyrolysates ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In this study, application of a fused silica capillary column for analysis of major mutagenic heterocyclic compounds found in cooked foods was investigated.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240071003
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  • 80
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    Valveless flow switching using constant flow controllers (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 577-577 
    Details
    ISSN: 0935-6304
    Keywords: Multidimensional gas chromatography, MGC ; Deans System ; Temperature programming ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240071006
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  • 81
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    HPLC analysis of steroid esters (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 584-586 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Normal and reversed phases ; Steroid esters ; Long-acting contraceptive ; Injectable contraceptives ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240071010
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  • 82
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    HPTLC separation of PGF2α, PGE1, PGE2, PGA1 prostaglandins and quantitative determination in situ by induced fluorescence (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 593-595 
    Details
    ISSN: 0935-6304
    Keywords: High performance thin layer chromatography, HPTLC ; Fluorescence ; Prostaglandins ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240071013
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  • 83
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    Product news (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 601-604 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240071017
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  • 84
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    Solute focusing using the solvent effect  -  A mass spectrometric investigation of packed bed inlets (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 142-143 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Solute focussing ; Solvent effect ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070309
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  • 85
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    Determination of silanol groups on surface of sorbents for HPLC (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 153-154 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Silanized silica for HPLC ; Surface silanol groups ; Dimethylzinc tetrahydrofuranate ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070314
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  • 86
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    High performance liquid chromatography profiling of prostaglandins (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 156-157 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; HPLC profiling ; Prostaglandins ; High speed HPLC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070316
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  • 87
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    Multi-mode liquid chromatography of polystyrenes on phenyl-bonded phase (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 158-159 
    Details
    ISSN: 0935-6304
    Keywords: Multi-mode liquid chromatography ; Adsorption chromatography ; Size exclusion chromatography ; Phenyl-bonded phase ; Styrene oligomers ; Polystyrene polymers ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070317
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  • 88
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    Effect of column diameter on efficiency in capillary supercritical fluid chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 312-318 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical fluid chromatography ; Capillary column ; Column diameter ; Efficiency ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fused silica capillary columns with internal diameters from 100 to 25 μm were coated with SE-54 and evaluated under supercritical fluid chromatographic conditions using carbon dioxide as mobile phase. Experimental results compared well with theoretical predictions. At ten times the optimum mobile phase velocity and for a capacity factor, k of 3, efficiencies of 2300 to 5600 plates m-1 were obtained for column diameters of 100 to 25 μm, respectively.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070605
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    Open tubular column gas chromatography: Theory and practice. Milton L. Lee, Frank J. Yang Keith D. Bartle John Wiley & Sons, New York, 1984, X + 445 pp., US $ 48.00. (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 327-327 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070607
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  • 90
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    Deactivation of fused silica capillary columns with polymethylhydrosiloxanes (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 329-332 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Fused silica capillary columns ; Deactivation ; Polymethylhydrosiloxanes ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070608
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  • 91
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    Multidetection of pesticides in capillary gas chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 338-339 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Pesticides ; Multidetection ; ECD-NPSD-SSD ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070613
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  • 92
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    Product news (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 345-350 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070616
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    Editorial (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 355-355 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070702
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    Analysis of gases containing inorganic compounds and light hydrocarbons on fused-silica open-tubular columns prepared with a thick liquid phase filmEnlarged text of a paper presented at the 35th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Atlantic City, NJ, USA, March 5-9, 1984. (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 358-369 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Thick liquid phase films ; Natural and refinery gases ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Capillary columns having a thick liquid phase film and a low phase ratio permit the separation of low molecular mass compounds which would have a very small capacity factor on columns with a classical thin film. At the same time, the increased sample capacity allows conventional hot-wire thermal conductivity detectors to be used with such columns. The analysis of natural and refinery gases, containing both inorganic compounds and light (C1—C7) hydrocarbons, utilizing a combination of hot-wire and flame ionization detectors, is demonstrated.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/jhrc.1240070705
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  • 95
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    Optimization of capillary parameters for gas chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 382-388 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; HETP equation ; Optimum column parameters ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An HETP equation for the capillary column is developed that takes into account the dependence of gaseous diffusion on pressure, the compressibility of the mobile phase, together with the unique relationship between mobile phase velocity, and the resistance to mass transfer in the stationary phase. The equation is used to develop a procedure for column optimization and expressions are derived that allow the optimum column radius and optimum column length to be calculated for a given fixed inlet pressure. It is shown that fast, simple separations are optimally achieved using relatively short small diameter columns. Conversely, optimum performance for the separation of complex mixtures requiring higher efficiencies requires the use of long columns with relatively large diameters.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jhrc.1240070708
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    On the potential of CGC in triglyceride analysis (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 431-432 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Triglycerides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070716
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  • 97
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    Effect of stationary phase film thickness on efficiency in capillary supercritical fluid chromatography (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 423-428 
    Details
    ISSN: 0935-6304
    Keywords: Capillary column ; Supercritical fluid chromatography ; Stationary phase film thickness ; Efficiency ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fused-silica capillary columns with internal diameters of 50 μm were coated with 0.25 to 1.0 μm films of SE-54 and evaluated under supercritical fluid chromatographic conditions using carbon dioxide as mobile phase. Experimental results compared well with theoretical predictions. There was no significant difference in hmin or ūopt for film thicknesses from 0.25 to 1.0 μm over k = 1 to 5. At a film thickness of 1.0 μm, calculations indicate that approximately 10% resolution loss would be expected for solutes with k = 1.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070714
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  • 98
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    Electronic Resource
    Chromatography calendar (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 433-435 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070717
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  • 99
    Electronic Resource
    Electronic Resource
    Determination of nitrated-polyaromatic hydrocarbons (nitro-PAHs) in environmental samples by high resolution chromatographic techniques (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 389-397 
    Details
    ISSN: 0935-6304
    Keywords: Low and high resolution liquid chromatography ; Gas chromatography ; Capillary columns ; Alkali flame detection ; Chemical ionization mass spectrometry ; Nitro-PAHs ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An analytical procedure is described for the fractionation of organic compounds present in environmental samples and the determination of nitro polyaromatic hydrocarbons (nitro-PAHs). Both low and high resolution liquid chromatography are employed for the prefractionation of the soluble organic fraction (SOF) extracted from particulate matter or gaseous pollutants collected on adsorption traps. High resolution gas chromatography is used to analyze four fractions containing alkanes, PAHs, nitro-PAHs, and other polar PAHs. Nitrogen-containing species are separated by GC and detected specifically using an alkali flame (NPD) detector. Flame ionization (FID) detection, GC-MS of positive ions, and negative ion chemical ionization MS of the whole fraction is used for the identification and quantitation of the various components. The composition of SOF extracted from particulate matter emitted from diesel exhausts is elucidated and a large number of nitro-PAHs identified by the combination of the various techniques.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070709
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  • 100
    Electronic Resource
    Electronic Resource
    HPLC of nucleotides. II. General methods and their development for analysis and preparative separation. An approach to selectivity controlThis review was presented at the Budapest Chromatography Conference, June 1983, as a report and two posters. Part of it was included in [1]. Abbreviations: r - denotes ribonucleotides; d - (deoxy) is omitted; p - denotes terminal phosphate groups; internucleotide phosphate groups also are omitted. (1984)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 7 (1984), S. 442-460 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; General methods of nucleotide HPLC ; Molecular and ionic basis of separation ; Two-dimensional separation ; Stationary phase; selectivity to nucleotide size and composition, selectivity control ; Mobile phase (solvent, salt, pH), influence on retention and selectivity ; Guard-column functions ; Column loadability and packing, consolidation of bed particles ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In order to develop efficient separation methods for nucleotides according to their size and heterocyclic composition, the application of ion-exchange, reverse-phase, and normal-phase adsorption HPLC has been studied. The comparative investigation of retention power and selectivity of various packings (non-polar bonded-phase and amino silicas) in relation to nucleotide length and composition yields data which enable suitable packings to be selected and a method of preparing the new packing for a particular separation problem to be formulated. Thus a new anion exchanger with high selectivity and dynamic mass transfer has been prepared for fractionation of large oligonucleotides. The effect of the eluent pH and composition (organic modifier, salt) on retention, selectivity, and resolution in ion-exchange and reverse-phase HPLC has been studied. The optimum separation conditions comprise elution with oppositely directed gradients of the salt and the modifier, use of a precolumn packing that provides the best protection for the main column without loss of its efficiency, and the optimum gradient program for the desired retention of the component of interest. The relation between loading and sample concentration has been studied and the system for gradient elution improved. Our work shows that two-dimensional separation is the most reliable and informative method for preparation of homogeneous oligonucleotides. The hydrophobic-pair ion-exchange mechanism is proposed for ion-pair chromatography. Protected and partially deblocked oligonucleotides, chemically synthesized for genetic engineering studies, have been separated with high selectivity by adsorption (normal-phase) HPLC which is efficient for gradient elution with isohydric eluents. The analysis of a monomeric composition of nano-(pico-) molar amounts of oligonucleotides has been developed; the procedure involves microcolumn digestion of the oligonucleotides with immobilized enzymes followed by microcolumn separation of the nucleoside-mononucleotide mixture. Also, a new slurry method for packing stable HPLC columns with a tightly consolidated, nonshrinkable bed of particles has been developed.
    Additional Material: 31 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240070802
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