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  • 1995-1999  (457)
  • 1955-1959
  • 1997  (457)
  • Chemical Engineering  (418)
  • transformation
  • 1
    ISSN: 1572-8773
    Keywords: NMR and EPR spectroscopy ; speciation ; transformation ; vanadate ; vanadyl ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three strains of Saccharomyces cerevisiae, SC-1, DBVPG 6173 and DBVPG 6037, were studied for vanadate resistance in complex Sabouraud medium since they did not thrive in different minimal media (yeast nitrogen base with and without amino acids). The strain SC-1 was resistant up to 16 mm of vanadate, whereas the strains DBVPG 6173 and DBVPG 6037 were inhibited by 8 mm and 4 mm vanadate, respectively. The vanadate resistance in strain SC-1 was constitutive and due to the reduction of this oxyanion to vanadyl, which was detected by EPR spectroscopy and visible spectroscopy. The transformation of vanadate to vanadyl took place during the exponential growth phase; 10 mm of vanadate was reduced to vanadyl outside the cells since the oxyanion was not detected in the cell biomass and only a negligible concentration of vanadyl (25 nmoles mg cells dry weight) was found in the biomass. The other two vanadate-sensitive yeast strains only accumulated vanadate and did not reduce the oxyanion to vanadyl.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Catalysis letters 49 (1997), S. 121-128 
    ISSN: 1572-879X
    Keywords: cresols ; transformation ; isomerization ; disproportionation ; zeolites HY
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The transformation of m- and o-cresol has been investigated on a series of HY zeolites with Si/Al ratio in the range of 4.5 to 55, at 380°C and atmospheric pressure. It was found that the zeolite activity increased but stability decreased with NAl for both m- and o-cresol. For Si/Al = 4.5 the zeolite activity was similar for both reactants, but as Si/Al ratio increased, o-cresol became relatively more reactive than m-cresol, and for all Si/Al ratios the catalysts were more stable in the m-cresol transformation. Both m- and o-cresol were converted mainly through two parallel reactions - isomerization and disproportionation. The disproportionation reaction was predominant, and more important in the case of o-cresol. The isomerization/disproportionation selectivity increased for m-cresol and decreased for o-cresol with NAl. Catalyst deactivation favoured disproportionation in the case of o-cresol and isomerization in the case of m-cresol, except for o-cresol conversion on HY(4.5), where both reactions were equally affected. Isomerization selectivity for m-cresol (p/o) was found to be higher than the equilibrium value and to increase with catalyst deactivation; while for o-cresol, the isomerization ratio (m/p) did not change with time on stream for HY(4.5) and HY(16.6) and decreased for HY(55). Disproportionation selectivity (ph/∑x) was equal to unity independently of Si/Al ratio or catalyst deactivation.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International journal of value-based management 10 (1997), S. 9-29 
    ISSN: 1572-8528
    Keywords: business ethics ; Jewish ; religious ; community ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper examines the method of Jewish business ethics. MichaelWalzer, in his work, Interpretation and Social Criticism (1987), suggeststhree common and important approaches to moral philosophy. He labels thesethe path of discovery, the path of invention, and the path ofinterpretation. The first part of this paper argues that Jewish businessethics is best thought of in terms of interpretation. Without question, thereligious ethicist immediately recognizes Walzer‘s metaphor of the moralworld as a ’home occupied by a single family over many generations... ‘as his own. Ethical arguments from a Jewish perspective must of necessityhave a ’lived-in quality‘ and always make reference to and are based on the’memory-laden objects and artifacts.‘ The second part of the paper exploressome of the implications of Jewish business ethics as interpretation.
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  • 4
    ISSN: 1432-0789
    Keywords: Cyanobacteria ; Soil inoculation N ; transformation ; Inorganic N ; Easily oxidizable N ; Hydrolysable N ; Non-hydrolysable N ; Wetland rice Farmyard manure ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted with wetland rice (Oryza sativa cv. IR-36) in a sandy clay loam soil (Entisol) to study the effect of inoculation with a soil-based mixed culture of four diazotrophic cyanobacteria,Aulosira fertilissima, Nostoc muscorum, N. commune andAnabaena spp., on the N-flux in inorganic NH4 ++NO3 −+ NO2 −), easily oxidizable, hydrolysable and non-hydrolysable forms of N in soil during vegetative growth periods of the crop. Effects on grain and straw yield and N uptake by the crop were estimated. The effects of applying urea N and N as organic sources, viz.Sesbania aculeata, Neem (Azardirachta indica) cake and FYM, each at the rate of 40 kg N ha−1, to the soil were also evaluated. Inoculation significantly increased the release of inorganic N, evidenced by its increased concentrations either in soil or in soil solution. However, such increases rarely exceeded even 4% of total N gained in different froms in the soil system by inoculation during the vegetative growth stages of the rice plant, when the nutritional requirement of the plants is at a maximum. Most of the N2 fixed by cyanobacteria remained in the soil as the hydrolysable form (about 85%) during this period. Inoculation caused an insignificant increase in grain (8%) and straw (11%) yield, which was, however, accompanied by a significant increase in N uptake by the grain (30%) and an increase in total uptake of 15.3 kg N ha 1. Such beneficial effects of inoculation varied in magnitude with the application of organic sources, with farmyard manure (FYM) being the most effective. Application of urea N, on the other hand, markedly reduced such an effect.
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  • 5
    ISSN: 1572-9818
    Keywords: Agrobacterium ; Populus ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In recent years, Populus species have acquired an important place in basic and applied research of woody plants. The practical role of Populus species in world forestry and their importance to research as a woody-plant model have led to increasing interest in tissue-culture and molecular techniques, as well as the development of transformation procedures for this genus. A simple technical procedure is described here step-by-step, for the first time, as a routine method for transforming Populus tremula using a disarmed Agrobacterium tumefaciens hypervirulent strain. The procedure begins with the inoculation of stem explants with bacterial suspension, followed by a short period of co-cultivation on a highly regenerative medium. Transformed shoots are selected on regeneration medium containing antibiotics and the presence of the inserted target genes is checked using a rapid and efficient PCR test. Selected shoots are transferred to a rooting medium, under the same selection pressure, and propagated via stem cuttings. Selected plants can be hardened and transferred to the green-house within 4 months of inoculation. The method has proven efficient for several gene constructs, selection on Kan or Hyg, and three different Agrobacterium strains.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology reporter 15 (1997), S. 209-215 
    ISSN: 1572-9818
    Keywords: selectable marker ; cereals ; leaf assay ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A very simple leaf assay is described that rapidly and reliably identifies transgenic plants expressing the hygromycin resistance gene, hph or the phosphinothricin resistance gene, bar. Leaf tips were cut from plants propagated either in the glasshouse or in tissue culture and the cut surface embedded in solid medium containing the appropriate selective agent. Non-transgenic barley or rice leaf tips had noticeable symptoms of either bleaching or necrosis after three days on the medium and were completely bleached or necrotic after one week. Transgenic leaf tips remained green and healthy over this period. This gave unambiguous discrimination between transgenic and non-transgenic plants. The leaf assay was also effective for dicot plants tested (tobacco and peas).
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  • 7
    ISSN: 1572-9818
    Keywords: Junction sequence ; transformation ; Agrobacterium tumefaciens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic integration of transferred T-DNA is traditionally analyzed by Southern hybridization; however, these analyses often do not provide sufficient information pertaining to the transformation event. Analysis of the junction sequences spanning the region between the T-DNA borders and plant genomic DNA, give a clear demonstration of genomic integration. The procedures available for border junction analysis can be problematic, therefore a simplified method was developed for plants transformed by Agrobacterium tumefaciens harboring the binary vector with pBI121 backbone.
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  • 8
    ISSN: 1573-4919
    Keywords: endothelin ; fibroblasts ; receptors ; transformation ; oncogene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Endothelin-1 (ET-1), a peptide isolated from the culture medium of endothelial cells, mediates a variety of physiological and pathological responses including mitogenesis. We have compared the expression of ET receptors in untransformed versus ras-transformed NIH-3T3 murine fibroblasts and in untransformed versus SV40-transformed Wl38 (VA13) human fibroblasts by ligand binding and Northern analysis. NIH-3T3 and Wl38 cells displayed high affinity (200 and 220 pM) and high density (23,000 sites/cell and 14,000 sites/cell for NIH-3T3 and Wl38 cells, respectively) ET receptors. Competition binding experiments using subtype-selective ligands identified these receptors as the ETA subtype. Addition of ET-1 to the cells produced a concentration-dependent increase in intracellular calcium release. Both ras-transformed NIH-3T3 cells and SV40-transformed Wl38 cells (VA13) completely lacked [125I]ET-1 binding and failed to release calcium when exposed to ET-1. Northern analysis of the polyadenylat ed RNA (polyA RNA) isolated from untransformed and transformed cells revealed that the steady-state level of ETA receptor RNA was 90-95% less in transformed cells compared to untransformed cells. Thus, the loss of ET receptors as well as the receptor-mediated responses in transformed cells can be explained by down-regulation of ET receptor mRNA.
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  • 9
    ISSN: 1573-5028
    Keywords: CaMV 35S promoter ; Catharanthus roseus ; FMV 34S promoter ; particle bombardment ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Activities of several CaMV 35S and FMV 34S promoter derivatives fused to the gusA reporter gene were compared in suspension-cultured Catharanthus roseus cells that were transiently and stably transformed using particle bombardment. Our data demonstrate that the 35S and a deletion derivative of the 34S promoter combined with particle bombardment form useful tools for genetic engineering of C. roseus cells. Our results disagree on several points with activities of 35S and 34S promoter derivatives reported for tobacco, indicating that absolute and relative promoter activities can differ between plant species.
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  • 10
    ISSN: 1573-9368
    Keywords: Ac ; Agrobacterium ; aspen ; Populus ; transformation ; transposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aspen (Populus tremula) and hybrid aspen (P. tremula × P. tremuloides) were transformed with different gene constructs using two types of promoter. The aim was to determine the influence of the reporter gene rolC, controlled by promoters of viral or plant origin, on genetic and morphologic expression of different transgenic aspen clones. An improved transformation method using leaf discs was developed, by which putative transgenic plantlets were regenerated at high efficiencies (up to 34%) on kanamycin-containing medium. Transgenic aspen carrying the rolC gene from Agrobacterium rhizogenes under control of the cauliflower-35S-promoter are reduced in size with smaller leaves, whereas aspen transgenic for the same rolC gene, but under control of the light inducible rbcS promoter from potato, are only slightly reduced in size compared to untransformed controls. However, all clones carrying 35S-rolC and rbcS-rolC genes revealed light-green colouration of leaves when compared to untransformed aspen. Owing to this special feature, constructs were used in which expression of the rolC gene was inhibited by insertion of a transposable element, Ac, from maize. Transgenic aspen transformed with the 35S-Ac-rolC and rbcS-Ac-rolC genes were morphologically similar to untransformed aspen, but out of 54 independently regenerated 35S-Ac-rolC transgenic aspen clones, 30 clones showed light-green/dark green variegated leaves. In contrast, out of 19 independently transformed rbcS-Ac-rolC aspen clones, only two clones revealed light-green/dark green variegated leaves. The role of bacterial strains in transformation, and molecular genetics of transgenic aspen plants (including the function of the transposable element, Ac, in the aspen genome) are discussed
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  • 11
    ISSN: 1573-9368
    Keywords: peanut ; engineered virusresistance ; tomato spotted wiltvirus ; transformation ; nucleocapsid ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucleocapsid gene of tomato spotted wilt virus Hawaiian L isolate in a sense orientation, and the GUS and NPTII marker genes, were introduced into peanut (Arachis hypogaea cv. New Mexico Valencia A) using Agrobacterium-mediated transformation. Modifications to a previously defined transformation protocol reduced the time required for production of transformed peanut plants. Transgenes were stably integrated into the peanut genome and transmitted to progeny. RNA expression and production of nucleocapsid protein in transgenic peanut were observed. Progeny of transgenic peanut plants expressing the nucleocapsid gene showed a 10- to 15-day delay in symptom development after mechanical inoculations with the donor isolate of tomato spotted wilt virus. All transgenic plants were protected from systemic tomato spotted wilt virus infection. Inoculated non-transformed control plants and plants transformed with a gene cassette not containing the nucleocapsid gene became systemically infected and displayed typical tomato spotted wilt virus symptoms. These results demonstrate that protection against tomato spotted wilt virus can be achieved in transgenic peanut plants by expression of the sense RNA of the tomato spotted wilt virus nucleocapsid gene
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  • 12
    ISSN: 1573-9368
    Keywords: transgenic cotton ; bialaphos ; particle bombardment ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Resistance to bialaphos, a non-selective herbicide, was intro duced into cotton through genetic engineering. A gene encoding phosphinothric in acetyltransferase (bar) from Streptomyces hygroscopicus was inserted into elite varieties of cotton through particle bombardment. Based on the marker gene, β-glucuronidase (gus) expression, a total of 18 Pima (Gossypium barbadense), 45 DP50 (G. hirsutum L.), 20 Coker 312 (G. hirsutum) and 2 El Dorado (G. hirsutum) transgenic plants were recovered. Integration of the bar gene into cotton genomic DNA was confirmed by Southern blot analysis and gene expression was confirmed by northern blot and enzyme assays. Herbicide (Basta®) tolerance up to 15 000 ppm was demonstrated in greenhouse trials. The newly introduced herbicide tolerance trait is inherited in a Mendelian fashion in the progenies of germline transformants. This study demonstrates the potential for particle bombardment to introduce commerically important genes directly into elite varieties of cotton. This mode of gene transfer can expedite the introduction of transgenic cotton products into world markets
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Transgenic research 6 (1997), S. 279-288 
    ISSN: 1573-9368
    Keywords: Agrobacterium tumefaciens ; Brassica napus ; wintercultivar ; transformation ; GUS ; hygromycin ; kanamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient protocol for Agrobacterium tumefaciens-mediated transformation of six commercial Brassica napus winter cultivars is described. Two B. napus spring cultivars were analysed for comparison. Five strains of A. tumefaciens with different combinations of nopaline and octopine chromosomal backgrounds and virulence plasmids were used for cocultivation. Selection of putative regenerated transgenic plants was performed on kanamycin- or hygromycin-containing media. The scores of transgenic plants were calculated on the basis of GUS (β-glucuronidase) activity, detected by the histochemical X-Gluc test. Target tissue derived from the cut surface of cotyledon petioles resulted in successful transformation with all the winter cultivars tested. Target tissue from hypocotyl segments resulted in a successful transformation with only one winter cultivar. The transformation rates for B. napus winter cultivars in this study were higher than in previous reports. Southern blot analysis revealed that integration of marker genes occurred in single and in multiple copies and at multiple loci in the genome. The transgenic plants all grew normally and developed fertile flowers after a vernalization period. After self-pollination, Southern blot analysis of selected GUS active F1 plants revealed that introduced marker genes were stably inherited to the next generation. These data demonstrate that morphologically normal, fertile transgenic plants of B. napus winter cultivars can be achieved with both nopaline- and octopine-derived A. tumefaciens strains. This protocol should have a broad application in improvement of Brassica napus winter cultivars by introduction of foreign genes
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  • 14
    ISSN: 1573-9368
    Keywords: transformation ; Erysiphe graminis ; powderymildew ; coleoptile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A transient assay is described that should allow evaluation of the role of host genes in disease response by enhancing or disrupting expression of those genes in specific cells and looking for effects on disease development. The assay also has the potential for assessing utility of host and non-host genes in enhancing resistance to disease in transgenic plants. Particle bombardment with a helium discharge particle gun was utilized to transiently express genes in epidermal cells of coleoptiles of barley (Hordeum vulgare). An anthocyanin reporter gene construct provided a means of identifying those cells that were transiently expressing introduced DNA. Optimal transient expression rates were achieved two days following bombardment with 1800 psi helium pressure, 1.0 μm diameter gold particles, and coleoptile pre- and post-treatment in 0.30--0.35 m mannitol/sorbitol. Under optimal conditions, at least 35 cells expressed anthocyanin per bombardment. Transiently expressing cells were inoculated with the fungal pathogen, Erysiphe graminis f. sp. hordei, and fungal development observed. Neither the bombardment procedures, the presence of nearby dead cells, nor accumulation of anthocyanin within living cells affected fungal development in living cells. Therefore, incorporation of disease-related genes onto the same plasmid as the reporter genes will allow evaluation of the role of those genes in disease development or suppression. Since particle bombardment is possible with a great range of different plant tissues, the described methodology should exhibit wide applicability for evaluating genes in diverse plant-pathogen interactions, as well as genes involved in many other biological processes
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Journal of porous materials 4 (1997), S. 165-169 
    ISSN: 1573-4854
    Keywords: ferrihydrite ; hematite ; BET ; porosity ; catalyst ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Ferrihydrite is a poorly-ordered iron oxyhydroxide which possesses a high specific surface area (〉200 m2/g). This makes it an important constituent for surface related processes in nature and catalysis. This study focuses on the precautions that have to be taken in the sample preparation prior to BET measurements in order to avoid essential structural transformations. The experiments showed that temperatures between 90 and 120°C are necessary to remove surface contaminations and simultaneously prevent structural transformation of the ferrihydrite during the outgassing procedure. Furthermore, care has to be taken to keep the humidity low in the immediate proximity of the sample, due to the strong impact of this parameter on the structural stability of ferrihydrite.
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  • 16
    ISSN: 1572-9788
    Keywords: Bacillus thuringiensis ; Chilo suppressalis ; biolistic ; rice ; Scirpophaga incertulas ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Rice cultivars of isozyme group V include high-quality, aromatic rices that are difficult to improve by traditional methods because of the loss of quality characters upon sexual hybridization. Their low-tillering plant type predisposes them to economic loss from attack by stem borers, a group of insects to which they are susceptible. We report here the enhancement of stem borer resistance in cv. Tarom Molaii through transformation by microprojectile bombardment. Embryogenic calli derived from mature seeds were bombarded with gold particles coated with plasmid pCIB4421, carrying a synthetic truncated toxin gene based on the cryIA(b) gene from Bacillus thuringiensis, and plasmid pHygII, carrying the hygromycin phosphotransferase (hpt) selectable marker gene. Inclusion of 50 mg/l hygromycin B in culture media from bombardment through to rooting of plantlets eliminated escapes. The procedure generated three independent hpt transformants of which two also contained the cryIA(b) gene. One such line (No. 827) produced truncated (67 kDa) CryIA(b) protein equivalent to about 0.1% of total soluble protein. The cryIA(b) gene was controlled by the promoter of the maize C4 PEP carboxylase gene and was expressed in leaf blades but was not expressed to a detectable level in dehulled mature grain. Line 827 contained about 3 copies of the cryIA(b) gene which segregated as a single dominant Mendelian locus in the second (T1) and third (T2) generations and co-segregated with enhanced resistance to first-instar larvae of striped stem borer (Chilo suppressalis) and yellow stem borer (Scirpophaga incertulas). T2 line 827-6 homozygous for the cryIA(b) gene showed no dead hearts or whiteheads after infestation with stem borers, whereas T2 line 827-25 lacking the gene averaged 7 dead hearts per plant and 2.25 whiteheads per plant. These results establish that transformation of high-quality rices of group V is a feasible alternative to sexual hybridization.
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  • 17
    ISSN: 1572-9788
    Keywords: adventitious root formation ; Agrobacterium tumefaciens ; A. rhizogenes ; rootstock ; rose ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic plants of the rootstock Rosa hybrida L. cv. Moneyway were produced via a two-step procedure. First, kanamycin-resistant roots were generated on stem slices from micropropagated shoots, which were cocultivated with Agrobacterium tumefaciens containing the neomycin phosphotransferase II (NPTII) gene for conferring kanamycin resistance, together with individual ROL genes from A. rhizogenes. Root formation was quite efficient and up to two kanamycin-resistant roots per stem slice were produced. In the second step, these roots were used to regenerate transgenic plants via somatic embryogenesis. Although regeneration lasted up to 12 months, production of several transformants was successfully accomplished. Untransformed escapes were not found, indicating that the initial selection on kanamycin resistance was reliable. The presence of a combination of ROLA, B and C genes enhanced adventitious root formation on micropropagated shoots and explants of stems and leaves. It appears that the auxin sensitivity was increased to such a degree that cells were able to respond even to endogenous auxins present in shoots and leaves. Rooting experiments in greenhouse demonstrated that adventitious root formation on cuttings was improved threefold upon introduction of these ROL genes. It is concluded that a method was developed for the production of ROL gene transformed roses with improved rooting characteristics.
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  • 18
    ISSN: 1573-5028
    Keywords: competitive PCR ; flavonoid pathway ; Forsythia ; gene expression ; transformation ; woody ornamentals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression, during flower development, of the gene encoding the anthocyanin pathway key enzyme dihydroflavonol 4-reductase (DFR) was investigated in floral organs of Forsythia × intermedia cv. ‘Spring Glory’. Full-length DFR and partial chalcone synthase (CHS) cDNAs, the gene of interest and a flavonoid pathway control gene respectively, were obtained from petal RNA by reverse transcription PCR. Whereas for CHS northern blot analysis enabled the study of its expression pattern, competitive PCR assays were necessary to quantify DFR mRNA levels in wild-type plants and in petals of 2 transgenic clones containing a CaMV 35S promoter-driven DFR gene of Antirrhinum majus. Results indicated a peak of CHS and DFR transcript levels in petals at the very early stages of anthesis, and different expression patterns in anthers and sepals. In comparison to wild-type plants, transformants showed a more intense anthocyanin pigmentation of some vegetative organs, and a dramatic increase in DFR transcript concentration and enzymatic activity in petals. However, petals of transformed plants did not accumulate any anthocyanins. These results indicate that other genes and/or regulatory factors should be considered responsible for the lack of anthocyanin production in Forsythia petals.
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Pharmaceutical research 14 (1997), S. 750-756 
    ISSN: 1573-904X
    Keywords: DNA delivery ; cells ; sonication ; cavitation ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. Ultrasonic methods have considerable potential for the introduction of macromolecules into cells. In this paper we demonstrate that, under controlled conditions, application of 20kHz ultrasound to a suspension of yeast cells facilitates the delivery of plasmid DNA into these cells. Methods. Aliquots of growing yeast cells (Saccharomyces cerevisae, strain AH22) were suspended in buffer and exposed to 20kHz ultrasound from a laboratory (probe-type) sonicator in the presence of microgram quantities of plasmid DNA. Efficiency of DNA delivery was scored as the number of cells transformed. Results. Cell transformation was optimal at 30 seconds sonication using an output of 2.0 watts and resulted in a 20 fold enhancement over control values. At extended sonication times, fewer cells showed evidence of transformation because of reduced cell viability. The increased DNA uptake and the decreased cell viability were both attributable to acoustic cavitation events during sonication. The extent of acoustic cavitation was measured and it was found that there was an increase in cavitation events with increased sonication time. Cell viability was shown to be directly related to the number of cavitation events. The effects of sonication on plasmid DNA were investigated and indicated that the structural integrity of plasmid DNA was unaffected by the sonication conditions employed. Conclusions. Under controlled conditions, ultrasound is an effective means of delivering plasmid DNA into cells. The subsequent expression of DNA molecules in cells depends upon a balance between transient cell damage and cell death.
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 94 (1997), S. 83-91 
    ISSN: 1573-5060
    Keywords: Agrobacterium tumefaciens ; Beta vulgaris ; 5-enolpyruvylshikimate-3-synthase ; glyphosate ; herbicide ; sugar beet ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sugar beet (Beta vulgaris L.) lines transformed with the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium sp. CP4 and a glyphosate oxidase reductase gene (GOX) also isolated from bacteria resulted in the development of lines highly tolerant to glyphosate. Glyphosate (N-phosphonomethyl-glycine) is the active ingredient in Roundup®, herbicide. The EPSPS enzyme is involved in the biosynthesis of aromatic amino acids. Glyphosate binds irreversible to the EPSPS and inhibits the pathway. GOX degrades glyphosate into non-toxic compounds. 260 independent transformants have been evaluated in greenhouse and field trials for tolerance to Roundup® in 1993 and 1994. Variation of tolerance was recorded between different transformants, ranging from complete susceptibility to full tolerance. The Agrobacterium tumefaciens mediated transformation resulted in a negative correlation between copy number of the T-DNA insert and the level of tolerance to the herbicide. Transformants which contain a single copy insert showed tolerance to higher doses of glyphosate than transformants with multiple copies. Two transgenic lines were identified that showed agronomically useful tolerance to glyphosate.
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  • 21
    ISSN: 1573-5079
    Keywords: Chlamydomonas reinhardtii ; mutagenesis ; photoinhibition ; Photosystem II ; repair cycle ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The work outlines the isolation of transformant Chlamydomonas reinhardtii cells that appear to be unable to repair Photosystem II from photoinhibitory damage. A physiological and biochemical characterization of three mutants is presented. The results show differential stability for the D1 reaction center protein in the three mutants compared to the wild type and suggest lesions that affect different aspects of the Photosystem II repair mechanism. In the ag16.2 mutant, significantly greater amounts of D1 accumulate in the thylakoid membrane than in the wild type under steady-state growth conditions, and D1 loss is significantly retarded in the presence of the protein biosynthesis inhibitor chloramphenicol. Moreover, aberrant electrophoretic mobility of D1 in the ag16.2 suggests that this protein is modified to an as yet unknown configuration. These results indicate that the biosynthesis and/or degradation of D1 is altered in this strain. A different type of mutation occurred in the kn66.7 and kn27.4 mutants of C. reinhardtii. The stability of D1 declined much faster as a function of light intensity in these mutants than in the wild type. Thereby, the threshold of photoinhibition in these mutants was significantly lower than that in the wild type. It appears that kn66.7 and kn27.4 are similar conditional mutants, with the only difference between them being the amplitude of the chloroplast response to the mutation and the differential sensitivity they display to the level of irradiance.
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  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 49 (1997), S. 71-74 
    ISSN: 1573-5044
    Keywords: cocultivation ; monocot ; regeneration ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root explants from in vitro-grown plants of Anthurium andraeanum ‘Alii’ and Anthurium interspecific hybrid ‘UH1060’ readily produced multiple shoots under weak light on a modified Murashige & Skoog medium containing 2.2 μM BA. Regenerated ‘UH1060’ plants grew normally and flowered within 16 months after transfer to the greenhouse. Cocultivation of root cuttings with Agrobacterium tumefaciens LBA4404 carrying the binary vector pCa2Att resulted in kanamycin-resistant ‘Anuenue’ shoots transgenic for neo and att and recovered more than one year after culture on selection media. Transformation efficiency (number of explants with transgenic shoots per total explants) was 1.3%. Other cultivars (‘UH1003’, ‘UH1060’, ‘Rudolph’, and ‘Mauna Kea’) failed to produce shoots under the transformation condition employed.
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  • 23
    ISSN: 1573-5044
    Keywords: Agrobacterium ; coat protein ; grapevine ; hairy root ; nepovirus ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy root cultures of grapevine were obtained from plantlets co-inoculated by virulent Agrobacterium rhizogenes strains and disarmed A. tumefaciens strains harbouring the binary vectors pKHG4 and pKVHG 2+. These plasmids contain the nptII, hpt and gus genes and differ for the presence of the gene encoding for the grapevine chrome mosaic virus coat protein. For the cultivar ‘Gravesac’, 72% of the excised root tips initiated hairy root cultures on growth regulator-free media. According to the nature of the strains used in co-inoculation, co-transformation frequencies of the hairy root clones ranged from 4 to 16%. Co-transformed roots showed resistance to kanamycin and hygromycin but responses varied from clone to clone. Fluorometric GUS expression and GCMV coat protein production showed a large variability among hairy root clones co-transformed by pKHVG2+. Though the presence of gus, nptII and GCMV coat protein genes was checked by polymerase chain reaction and Southern blotting, it was difficult to establish a clear relationship between expression of the different transgenes. The regeneration of plants was not achieved, but the possibility to graft in vitro transgenic roots to non transformed shoot systems could permit rapid testing of the resistance induced by nepovirus coat protein in roots of cultivars that are recalcitrant to A. tumefaciens-mediated transformation.
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  • 24
    ISSN: 1573-6857
    Keywords: Anopheles albimanus ; malaria vector ; transformation ; white gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have cloned and characterized the white gene of Anopheles albimanus. Comparison of the deduced amino acid sequence of this white gene with its homologs from six species of Diptera show that the An. albimanus gene is most similar to the white gene of An. gambiae (92% identity). A spontaneous white-eyed mutant An. albimanus was caused by an approximately 10 kb insertion into a CT dinucleotide repeat region of intron 2 of the white locus. The flanks of this insertion are long (at least 400 bp), nearly perfect inverted terminal repeat sequences. This cloned white gene should be useful as a marker for germ line transformation of An. albimanus.
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  • 25
    ISSN: 1572-994X
    Keywords: adenovirus type 12 ; E1A ; transformation ; p300 binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The early region 1A (E1A) gene of highly oncogenic adenovirus type 12 (Ad12) was analyzed for transforming activity and protein binding using specific mutations. The Ad12 E1A proteins were found to bind p300 protein mainly within the CR1 region, although mutations that affect both p300 binding and transformation were identified in both the CR1 and the N-terminal region. The most critical mutation dlf89 located in the CR1 region was further dissected by point mutations and the results identified 68S as the most critical for transformation and 67E as the most critical for p300 binding. Specific mutations that retain p300 binding but impair transcriptional repression of a viral enhancer were also identified in both the N-terminal and CR1 regions.
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  • 26
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    Euphytica 96 (1997), S. 153-161 
    ISSN: 1573-5060
    Keywords: cassava ; transformation ; review ; somatic embryogenesis ; adventitious shoot formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A prerequisite for the development of a successful transformation system is the availability of efficient regeneration systems. Up to 1995 the only available regeneration system in cassava was an organized type of somatic embryogenesis. Transformation of these organized somatic embryogenic cultures with particle bombardment or Agrobacterium tumefaciens resulted in chimeric transformed embryos. However, the transformed sector was lost after repeated cycles of secondary somatic embryogenesis. After 1995 a less organized system of somatic embryogenesis was developed, so called friable embryogenic callus (FEC) and a system of adventitious shoot regeneration. The FEC regeneration system was combined successfully with particle bombardment. Selection of transgenic plants was based on either luciferase activity, or resistance to the aminoglycoside paromomycin or the herbicide phosphinothricin. Furthermore, protoplasts of FEC are able to regenerate into plants and can be transformed by electroporation. The adventitious shoot regeneration system was combined successfully with Agrobacterium tumefaciens. For this mature somatic embryos were cocultivated with Agrobacterium and cultured for adventitious shoot development. After selection based on the aminoglycoside geneticin or on hygromycin transgenic plants were formed.
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  • 27
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    Euphytica 96 (1997), S. 167-172 
    ISSN: 1573-5060
    Keywords: Coffea spp. ; coffee ; genetic ; in vitro ; regeneration ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most important advances obtained on in vitro coffee regeneration systems and in coffee genetic transformation, drawing perspectives and scopes to further studies in these fields are presented and discussed.
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  • 28
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    Plant molecular biology 35 (1997), S. 205-218 
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; rice ; transformation ; monocotyledons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium tumefaciens has been routinely utilized in gene transfer to dicotyledonous plants, but monocotyledonous plants including important cereals were thought to be recalcitrant to this technology as they were outside the host range of crown gall. Various challenges to infect monocotyledons including rice with Agrobacterium had been made in many laboratories, but the results were not conclusive until recently. Efficient transformation protocols mediated by Agrobacterium were reported for rice in 1994 and 1996. A key point in the protocols was the fact that tissues consisting of actively dividing, embryonic cells, such as immature embryos and calli induced from scutella, were co-cultivated with Agrobacterium in the presence of acetosyringonc, which is a potent inducer of the virulence genes. It is now clear that Agrobacterium is capable of transferring DNA to monocotyledons if tissues containing ‘competent’ cells are infected. The studies of transformation of rice suggested that numerous factors including genotype of plants, types and ages of tissues inoculated, kind of vectors, strains of Agrobacterium, selection marker genes and selective agents, and various conditions of tissue culture, are of critical importance. Advantages of the Agrobacterium-mediated transformation in rice, like on dicotyledons, include the transfer of pieces of DNA with defined ends with minimal rearrangements, the transfer of relatively large segments of DNA, the integration of small numbers of copies of genes into plant chromosomes, and high quality and fertility of transgenic plants. Delivery of foreign DNA to rice plants via A. tumefaciens is a routine technique in a growing number of laboratories. This technique will allow the genetic improvement of diverse varieties of rice, as well as studies of many aspects of the molecular biology of rice.
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  • 29
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    Water, air & soil pollution 95 (1997), S. 35-43 
    ISSN: 1573-2932
    Keywords: humic acid ; mercury ; transport ; transformation ; soil-plant system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The influence of humic acid (HA) on the transport and transformation of mercury (Hg) in soil was studied. No available Hg could be detected (〈2.5 μg kg−1) in alluvial soil when the content of HA-carbon (HA-C) was higher than 0.2 g kg−1 although a large amount of Hg (8 μg kg−1) was applied to the soil. The available Hg decreased with the increase of HA in purple soil (r=0.735). There are significant correlations between HA concentration and organic Hg in the tested soils (r=0.974 for the purple soil and r=0.979 for the alluvial soil). The increase of HA results in decrease of Hg absorbed by plant from the soil. A loss of Hg from soil caused by microbes was observed.
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  • 30
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    Water, air & soil pollution 95 (1997), S. 35-43 
    ISSN: 1573-2932
    Keywords: humic acid ; mercury ; transport ; transformation ; soil-plant system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The influence of humic acid (HA) on the transport and transformation of mercury (Hg) in soil was studied. No available Hg could be detected (〈2.5 μg kg-1) in alluvial soil when the content of HA-carbon (HA-C) was higher than 0.2 g kg-1 although a large amount of Hg (8 μg kg-1) was applied to the soil. The available Hg decreased with the increase of HA in purple soil (r=0.735). There are significant correlations between HA concentration and organic Hg in the tested soils (r=0.974 for the purple soil and r=0.979 for the alluvial soil). The increase of HA results in decrease of Hg absorbed by plant from the soil. A loss of Hg from soil caused by microbes was observed.
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  • 31
    ISSN: 1573-2932
    Keywords: desorption ; ferrihydrite ; modelling ; phosphate ; TEM ; transformation ; wastewater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The presence of phosphate retards the transformation of ferrihydrite into crystalline products. Increasing phosphate from 0 to 1 mole % results in an order of magnitude decrease in the rate of transformation of ferrihydrite at pH 12. Levels of phosphate of ∼1 mol % suppress the formation of goethite (α-FeO(OH)) and result in the formation of a product consisting of η-Fe2O3. Higher levels of phosphate result in the ferrihydrite remaining amorphous, even after several hundred hours. Phosphate prevents formation of goethite by hindering the dissolution of ferrihydrite rather than by interfering with nucleation and growth of goethite in solution. The transformation rate of pure ferrihydrite is also strongly inhibited in the presence of dissolved phosphate. This is due to surface complexation. The transformation rate was measured at temperatures of 60 °C and 70 °C. The rate of transformation was found to be described by either (i) a solid-state reaction equation for powdered compacts or (ii) a zero-order reaction controlled by desorption. The transformation of the ferrihydrite matrix was accompanied by the loss of the phosphate trace component. X-ray diffraction indicates that no solid solution involving phosphate substitution into η-Fe2O3 is formed. Transmission electron microphotographs of the original precipitates containing phosphate confirm the presence of the phosphate and demonstrate its involvement in linking together extremely small particles of ferrihydrite.
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  • 32
    ISSN: 1573-2932
    Keywords: desorption ; ferrihydrite ; modelling ; phosphate ; TEM ; transformation ; wastewater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The presence of phosphate retards the transformation of ferrihydrite into crystalline products. Increasing phosphate from 0 to 1 mole % results in an order of magnitude decrease in the rate of transformation of ferrihydrite at pH 12. Levels of phosphate of ∼1 mol % suppress the formation of goethite (α-FeO(OH)) and result in the formation of a product consisting ofη-Fe2O3. Higher levels of phosphate result in the ferrihydrite remaining amorphous, even after several hundred hours. Phosphate prevents formation of goethite by hindering the dissolution of ferrihydrite rather than by interfering with nucleation and growth of goethite in solution. The transformation rate of pure ferrihydrite is also strongly inhibited in the presence of dissolved phosphate. This is due to surface complexation. The transformation rate was measured at temperatures of 60 °C and 70 °C. The rate of transformation was found to be described by either (i) a solid-state reaction equation for powdered compacts or (ii) a zero-order reaction controlled by desorption. The transformation of the ferrihydrite matrix was accompanied by the loss of the phosphate trace component. X-ray diffraction indicates that no solid solution involving phosphate substitution intoη-Fe2O3 is formed. Transmission electron microphotographs of the original precipitates containing phosphate confirm the presence of the phosphate and demonstrate its involvement in linking together extremely small particles of ferrihydrite.
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  • 33
    ISSN: 1572-9788
    Keywords: Agrobacterium tumefaciens ; bar gene ; grain legume ; Lupinus angustifolius ; phosphinothricin ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic plants of Lupinus angustifolius L. (cvs. Unicrop and Merrit) were routinely generated using Agrobacterium-mediated gene transfer to shoot apices. The bar gene for resistance to phosphinothricin (PPT, the active ingredient of the herbicide Basta) was used as the selectable marker. After co-cultivation, the shoot apex explants were transferred onto a PPT-free regeneration medium and their tops were thoroughly wetted with PPT solution (2 mg/ml). The multiple axillary shoots developing from the shoot apices were excised onto a medium containing 20 mg/l PPT. The surviving shoots were transferred every second week onto fresh medium containing 20 mg/l PPT. At each transfer, the number of surviving shoots decreased, until it stabilized. Indeed, some of these chimeric shoots surviving the PPT selection, eventually produced new green healthier axillary shoots which could be transferred to soil. This whole process took from 5 to 9 months after co-cultivation. Average transformation frequencies of 2.8% for cv. Unicrop and of 0.4% for the commercial cultivar Merrit were achieved. Molecular analysis of T0, T1, and T2 generations demonstrated stable integration of the foreign gene into the plant genome and expression of the integrated gene. Transformed plants of the T1 and T2 generations were resistant in glasshouse trials where the herbicide Basta (0.1 mg/ml) was sprayed onto whole plants. These results demonstrate that Agrobacterium-mediated gene transfer to preorganised meristematic tissue combined with axillary regeneration can form the basis of a routine transformation system for legume crop species which are difficult to regenerate from other explants.
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  • 34
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    World journal of microbiology and biotechnology 14 (1997), S. 271-276 
    ISSN: 1573-0972
    Keywords: Biodegradation ; 3-chlorobenzoic acid ; degradative plasmid ; Pseudomonas putida ; rec− Escherichia coli DH5 ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 35
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    World journal of microbiology and biotechnology 14 (1997), S. 7-10 
    ISSN: 1573-0972
    Keywords: Biodegradation ; morpholine ; plasmid ; Pseudomonas fluorescens ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A fast-growing Pseudomonas fluorescens CAS102, isolated by enrichment technique from polluted soil, effectively utilized morpholine as the energy source. The strain was able to grow in high concentrations of morpholine but accumulation of ammonia inhibited its growth and complete mineralization. The molar conversion ratio of morpholine to ammonia was 1:0.82. The organism harboured a single, multiple antibiotic- and heavy metal-resistance 140kb plasmid which was resistant to curing. Transformation studies showed that the morpholine degradative phenotype was expressed only in Pseudomonas putida and not in Escherichia coli. Growth studies on different degradative intermediates of morpholine suggested that plasmid-encoded genes were involved in the heterocyclic ring cleavage and the remaining reactions were mediated by chromosomal genes.
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  • 36
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    AIChE Journal 43 (1997), S. 104-117 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Computational fluid dynamics methods are used to provide three-dimensional simulations of a low-density polyethylene (LDPE) autoclave reactor under normal operating conditions. For the conditions used, the reactor is not very well mixed; thus, the common model approximation of a perfectly stirred reactor is not warranted. The simulations verify the sensitive nature of the polymerization reactors and indicate a need for optimizing operating parameters.
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  • 37
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    AIChE Journal 43 (1997), S. 118-126 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A rigorous approach was developed for the simulation of the decoking of an industrial cracking furnace. A one-dimensional heterogeneous reactor model, which accounts for the interfacial gradients between the process gas and the coke surface, was used to simulate reactor coils. Both the combustion and steam gasification of the coke layer were taken into account. The reactor model for the decoking was coupled with a detailed firebox simulation model. The initial profile of the coke layer thickness, required for the decoking calculations, was obtained by a run-length simulation. The evolution with time of the temperature distribution inside the cracking coil and in the furnace was generated simultaneously, which made it possible to understand the decoking operation in detail and to predict its duration accurately.
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  • 38
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    AIChE Journal 43 (1997), S. 157-165 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A semibatch flow scheduling strategy proposed by Teymour and Ray (1989, 1996) is evaluated for a polymerization reaction conducted in a pilot-plant reactor. The reaction used is the free radical terpolymerization of styrene, α-methyl styrene, and acrylic acid monomers initiated by an organic peroxide initiator and carried out in the presence of a reactive glycol ether solvent. This strategy was tested in both single batch and sequential semibatch modes. The process was shown to produce polymer of constant molecular weight properties and composition as inferred from acid number and monomer conversion measurements. This process could be used for obtaining polymer products from a semibatch reactor that are of comparable quality to CSTR products. Results indicate success of this process at meeting this objective; however, practical considerations relating to agitation and temperature control need to be properly addressed to ensure this success.
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  • 39
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    AIChE Journal 43 (1997), S. 127-134 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The steady-state simulation of a cocurrently cooled autothermal fixed-bed reactor was carried out using a 2-D heterogeneous mathematical model. The ammonia synthesis was chosen as a case study. Unlike the not-autothermal cocurrent reactor, which is unconditionally stable, the autothermal cocurrent reactor shows multiple steady states within a broad range of operating conditions. This finding, not reported in the literature, is explained through the mass transport from the bottom to the top of the reactor, associated with a feedback of energy. The feedback of heat, which is inherent to autothermal reactors, leads to an ignition-extinction phenomenon similar to that found in the countercurrent configuration. The influence of different parameters on the stability of the autothermal cocurrent reactor was analyzed. The regions where steady-state multiplicity occurs were compared with those presented by the autothermal countercurrent reactor. The influence of an additional heat exchanger on the reactor stability was considered.
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  • 40
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    AIChE Journal 43 (1997), S. 277-280 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
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  • 41
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    AIChE Journal 43 (1997), S. 265-267 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
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  • 42
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    AIChE Journal 43 (1997), S. 268-272 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 43
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    AIChE Journal 43 (1997), S. 339-344 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An experimental investigation was conducted to determine the boiling heat-transfer characteristics in a thermosyphon condenser-reboiler of air-separation plants. The test section was a plate-fin heat exchanger of practical size of 0.106 m width and 2.56 m length. The testing fluid was nitrogen. Experiments were carried out within a range of pressure from 150 to 300 kPa, submergence from 1.95 to 2.80 m, and heat flux up to 11 kW/m2 under constant wall-temperature condition. The liquid superheat at the onset of boiling was rather significant. The boiling heat-transfer coefficients obtained agreed well with Chen's correlation modified by Colburn's J factor within a mean deviation of 9.3%.
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  • 44
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    AIChE Journal 43 (1997) 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 45
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    AIChE Journal 43 (1997), S. 440-447 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The concept of robust design involves identification of design settings that make the product performance less sensitive to the effects of seasonal and environmental variations. This concept is discussed in this article in the context of batch distillation column design with feed stock variations, and internal and external uncertainties. Stochastic optimization methods provide a general approach to robust/parameter design as compared to conventional techniques. However, the computational burden of these approaches can be extreme and depends on the sample size used for characterizing the parametric variations and uncertainties. A novel sampling technique is presented that generates and inverts the Hammersley points (an optimal design for placing n points uniformly on a k-dimensional cube) to provide a representative sample for multivariate probability distributions. The example of robust batch-distillation column design illustrates that the new sampling technique offers significant computational savings and better accuracy.
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  • 46
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    AIChE Journal 43 (1997), S. 33-44 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Equations were derived that describe the dynamics of cake growth in cake filtration, and methods for their solutions were developed. In deriving the equations, the moving boundary nature of the cake formation process and the effect of fine particle retention were considered. It was shown that fine particle retention may contribute significantly to the decrease of cake permeability and thus alters the performance of cake filtration even if the amount of fine particles involved is small. Through numerical examples, it was also demonstrated that fine particle retention in filter cakes may cause serious errors in determining the constitutive relationships from filter test data.
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  • 47
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    AIChE Journal 43 (1997), S. 45-57 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fundamental characteristics of liquid-solid fluidization at high pressures (up to 21 MPa) and moderate temperatures (up to 89°C) are investigated. Properties under study include minimum fluidization velocity (umf), bed voidage at minimum fluidization, and bed expansion. Devices for in-situ measurements of physical properties of the liquid in the bed are developed, and measurements are carried out to quantify the pressure and temperature effects on the fluidization behavior. The results indicate that under high pressures and moderate temperatures, liquid-solid fluidization behavior is affected significantly by the variation of liquid density and viscosity with pressure. As the pressure increases, the liquid viscosity and density increase, yielding an increased drag force and buoyancy force on the particles, and hence a decreasing umf and an increasing bed expansion for a given liquid flow rate. An increase in temperature has an opposite effect on the physical properties of the liquid, increasing umf and decreasing the bed expansion for a given liquid flow rate. Various correlations proposed in the literature for umf and bed expansion, including those by Richardson and Zaki (1954) and Chitester et al. (1984), are applicable to high-pressure and high-temperature conditions when proper account is made of the liquid physical properties under bed operating conditions.
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  • 48
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    AIChE Journal 43 (1997), S. 58-63 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Asymptotic power series solutions for the mass-transfer enhancement factor for absorption of a gas component into a liquid where it undergoes irreversible instantaneous chemical reaction(s) with one and two liquid-phase reactants are developed in this work. The Padé technique is used to extend the region of applicability (accelerate the convergence) of the four-term asymptotic power series solutions. The resulting modified asymptotic expressions for the enhancement factor show excellent accuracy over a wide range and can be used to predict enhancement factors as low as 2 with an error of about 5% compared to the exact numerical solution. Predictions of these new asymptotic solutions are compared with experimental absorption data for H2S absorption into aqueous methyldiethanolamine and H2S absorption into aqueous mixtures of methyldiethanolamine and diethanolamine obtained in a laminar-jet absorber. The absolute mean deviations of the predictions from the experimental absorption data for the single and mixed amine solutions were 4.6% and 2.4%, respectively.
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  • 49
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    AIChE Journal 43 (1997), S. 166-172 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: When a trickle-bed reactor (TBR) is operated periodically, the bed is fed with liquid on and off, while the gas phase passes continuously. Rates and conversions could be higher than those corresponding to the steady-state operation. In the “dry cycles” the heat generated by the reaction can drive the vaporization of the liquid phase, and a much more rapid “gas phase” reaction may occur. There is a trade-off between the potential for hot-spot formation and the fact that overall rates are higher during cycling. A qualitative understanding of the phase transition in TBRs is obtained by a simple phenomenological model that takes into account different transport and reaction mechanisms occurring in a catalytic particle under different cycling conditions. A mathematical model is used to predict results.
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  • 50
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    AIChE Journal 43 (1997), S. 180-195 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Theoretical and engineering models for the thermodynamic properties of strong electrolyte solutions have advanced significantly since 1985. This progress is notable in the ability to calculate selected properties of single and mixed strong electrolyte solutions over a wide range of temperatures and compositions, including effects of various nonelectrolytes, solvents and supercritical components. Theoretical studies have begun to consider more realistic fundamental interactions between various components in these systems. There have been several successful conversions of theories based on the mean spherical approximation and perturbation methods into engineering equations, without large numbers of empirical parameters. Other theoretical models seem almost ready for application to real systems. The capability to estimate a wide variety of thermodynamic properties accurately with a consistent set of equations and a small number of adjustable parameters has been achieved by several groups over limited temperature and composition ranges. Much work remains to be done, however, to understand completely the interplay and relative importance of various contributing energy effects.
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  • 51
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    AIChE Journal 43 (1997), S. 212-217 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The NpT + test-particle method for the calculation of vapor-liquid equilibria by molecular simulations is extended to ternary mixtures. It is applied to the system methane + ethane + carbon dioxide, for which all binary molecular interaction models are available from previous work. Methane is described as one-center Lennard-Jones fluid, ethane as two-center Lennard-Jones fluid, and carbon dioxide as two-center Lennard-Jones plus point quadrupole fluid. The unlike interactions are treated in the same way as the binary mixtures, using two parameters for each binary interaction. No ternary parameters are introduced. Vapor-liquid phase equilibria are calculated for the ternary mixture at the following temperature-pressure pairs: 233.15 K - 2 MPa; 250.5 K - 2 MPa; and 250.5 K - 3.04 MPa. Comparison of the simulation data with experimental and equation-of-state results shows excellent agreement. Bubble and dew densities are also reported.
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  • 52
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    AIChE Journal 43 (1997), S. 232-242 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An efficient and economical low-pressure liquid chromatography process has been developed for paclitaxel recovery and purification directly from plant-tissue culture (PTC) broth. PTC broth is first diluted with ethanol to ensure padlitaxel dissolution and then passed through a column packed with a high-capacity polystyrene divinyl-benzene sorbent. A step increase in ethanol concentration in the mobile phase (ethanol:water) is used to concentrate and compress the taxane bands to as high as 29-fold of influent concentrations (about 1 mg/L). A recycle technique is then used to separate the concentrated paclitaxel band from other taxane bands, achieving 95% purity with more than 90% recovery and 99% purity with more than 80% recovery. In this process, the same low-pressure columns are used to capture, concentrate and purify paclitaxel. Theoretical predictions agree closely with the stepwise elution and recycle chromatography data. After validation, simulations are used to explore various design and operating alternatives. Analysis of the alternatives shows that the process cost can be further reduced by using higher feed concentration, larger loading volume, smaller particle size, and optimal gradient and recycle strategies.
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  • 53
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    AIChE Journal 43 (1997), S. 218-231 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: When polymers are added to a solution containing proteins, the protein may deposit as an amorphous precipitate or as crystals. Experimental evidence indicates that from a thermodynamic viewpoint, an amorphous precipitate should be treated as a liquid phase dense in the protein and crystals as true solids. To understand the conditions under which protein precipitation or crystallization may occur, Gibbs-ensemble Monte Carlo simulation and Gibbs-Duhem integration techniques are used to calculate the liquid-liquid and liquid-solid phase boundaries for protein precipitation induced by polymers. In these simulations, the system is modeled using the pseudo-one-component approximation with an appropriate potential of mean force. The critical point for fluid-fluid phase equilibrium disappears as the attractive part of the mean-force potential becomes very short ranged. The accuracy of the second-order Barker and Henderson perturbation theory is examined by comparing the phase diagram predicted using this method with the simulation results. Perturbation theory is able to predict the general trends observed in the simulations but not with quantitative accuracy. Perturbation theory is then used to examine a broader range of conditions for protein deposition.
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  • 54
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    AIChE Journal 43 (1997), S. 260-262 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 55
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    AIChE Journal 43 (1997), S. 935-945 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simulated moving bed (SMB) was operated for the separation of mixtures of 2-phenyl ethanol and 3-phenyl-1-propanol on columns packed with Zorbax C18 bonded silica, using a 60:40 (v/v) solution of methanol and water as the mobile phase. Series of four or eight columns were used. The experiments were carried out with low concentration mixtures, that is, under linear conditions. Band profiles of both compounds eluted from one of the columns during successive periods after steady state had been reached were recorded, as were the concentration histories at the extract and raffinate ports. These experimental results are compared to those predicted by two models: the linear ideal and the linear equilibrium-dispersive models of chromatography, applied to the SMB separator. These two models give excellent agreement between the experimental profiles and those calculated with the model. As expected, the profiles predicted by the ideal and the equilibrium-dispersive models differ only by the lack of dispersion in the profiles given by the former. The latter model is demonstrated to be a solid, reliable tool for further studies of the SMB design and optimization.
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  • 56
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    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: To study the influence of particle concentration on the hydrodynamics of bubble-column slurry reactors operating in the heterogeneous flow regime, experiments were carried out in 0.10, 0.19, and 0.38-m-dia. columns using paraffinic oil as the liquid phase and slurry concentrations of up to 36 vol. %. To interpret experimental results a generalization of the “two-phase” model for gas-solid fluid beds was used to describe bubble hydrodynamics. The two phase identified are: a dilute phase consisting of fast-rising large bubbles that traverse the column virtually in plug flow and a dense phase that is identified with the liquid phase along with solid particles and entrained small bubbles. The dense phase suffers backmixing considerably. Dynamic gas disengagement was experimented in the heterogeneous flow regime to determine the gas voidage in dilute and dense phases. Experimental data show that increasing the solid concentration decreases the total gas holdup significantly, but the influence on the dilute-phase gas holdup is small. The dense-phase gas voidage significantly decreases gas holdup due to enhanced coalescene of small bubbles resulting from introduction of particles. The dense-phase gas voidage is practically independent of the column diameter. The dilute-phase gas holdup, on the other hand, decreases with increasing column diameter, and this dependence could be described adequately with a slight modification of the correlation of Krishna and Ellenberger developed for gas-liquid systems.
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  • 57
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    AIChE Journal 43 (1997), S. 475-485 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Strong intraparticle diffusion resistance was observed and the apparent effectiveness factors were measured for the hydrogenation of 3-hydroxypropanal (HPA) to 1,3-propanediol (PD) over Ni/SiO2/Al2O3 catalyst pellets at 45 to 80°C and 2.60 to 5.15 MPa in a stirred reactor with a spinning basket. A mathematical model was proposed to describe the intraparticle diffusion and to estimate the effective diffusion coefficients of HPA, PD and hydrogen under reaction conditions by a maximum likelihood function of the apparent effectiveness factors of HPA and PD. The calculated concentration distributions of HPA, PD and hydrogen in catalyst particles reveal an excess of hydrogen at 80°C and higher temperatures, but an excess of HPA at 45°C. It results in a weak dependence of the consumption formation rate of HPA and PD on the partial pressure of hydrogen at high temperature.
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  • 58
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    AIChE Journal 43 (1997), S. 631-644 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Electrophoresis of a solute through a column in which its transport is governed by the convection - diffusion equation is described. Approximate solutions to the convection - diffusion equation in the limit of small diffusion are developed using perturbation methods. The diffusion coefficient and velocity are assumed to be functions of space and time such that both undergo a sudden change from one constant value to another within a thin transition zone that itself translates with a constant velocity. Two cases are considered: (1) the thickness ∊f of the transition zone is negligible compared to the diffusional length scale, so the zone may be treated as a singular boundary across which the diffusion constant and velocity suffer discontinuous changes; (2) the transition zone is considerably wider than the diffusional length scale, so the diffusion coefficient and velocity, although sharply varying, are smooth functions of position and time. A systematic perturbation expansion of the concentration distribution is presented for case 1 in terms of the small parameter ∊ = 1/Pe. A lowest order approximation is given for case 2. A suitably configured system analyzed here can lead to progressive accumulation, or focusing, of the transported solute. The degree of focusing in case 1 scales with ∊-1, whereas in case 2 it scales with (∊f∊)-1/2, and thus increases much more weakly with increasing Pe. A separation based on this concept requires development of materials and devices that allow dynamic tuning of the mass-transport properties of a medium. This would make it possible to achieve progressive focusing and separation of solutes, such as proteins and DNA fragments, in electrophoretic media with an unprecedented degree of control.
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  • 59
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    AIChE Journal 43 (1997), S. 681-692 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence on drying selectivity of the continuous-contact mode between a solid wetted with ternary mixtures and a gas stream was theoretically studied. The liquid mixtures, ethanol-isopropanol-water and water-ethanol-acetone, were used. A mathematical model describing a gas-phase-controlled process was developed, and the influence of the process variables was studied by simulations. In addition to the inlet composition of the moisture and temperature of the solid, gas composition has the most important effect on selectivity. Small changes of gas composition, either imposed or spontaneous, may modify completely the process trajectory. The extent of these effects depends on the ratio between the flow rates of inlet gas and liquid contained in the solid. Because of their effects on the evolution of temperature and composition, the operating pressure and energy sources other than convection are also useful in controlling the selectivity. Since all these variables determine the composition of the remaining liquid and thereby product quality, their influence should be predictable. The model may be a valuable tool for exploring the process, provided that drying is gas-phase-controlled.
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    AIChE Journal 43 (1997), S. 844-846 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
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    AIChE Journal 43 (1997), S. 577-587 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Complex mixing flows and mixing parameters are calculated to evaluate mixing quality on the basis of kinematic parameters together with a statistical analysis. The results allow for a comparative evaluation in terms of geometry and flow parameters. The evaluation is limited to two-dimensional flows with moving boundaries in periodic motion such as the flow in the cross section of a twin-cam mixer. The finite-element calculation requires a remeshing procedure for every time step with special techniques to upgrade mixing variables from one time step to the next. The relative mixing quality of single cam and co: or counterrotating cam devices are compared by evaluating segregation scale, length stretch, and efficiency. The numerical results exhibit a good correspondence with their experimental counterpart.
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    AIChE Journal 43 (1997), S. 615-624 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Resonant power absorption is an important phenomenon during microwave heating. The resonances that occur when plane electromagnetic waves are incident on infinitely long cylinders and slabs are investigated as a function of sample dimensions. For cylinders two kinds of incident waves are studied: TMz when the electric field is oriented along the axis of the cylinder and TEz when the magnetic field is oriented along the axis. At a resonant condition the overage power absorbed by the sample is a local maximum. Due to attenuation within the sample the resonances decrease in intensity as the sample size increases. Using the dielectric properties of water, resonances are found to be a function of the ratio of the sample dimension to the wavelength of radiation, λs, in the sample. For slabs of thickness L and integer values of n, resonances occurred at L/λs = 0.5n; for cylinders of diameter D, resonances occurred at D/λs = 0.5n - 0.257. The generality of these relations to predict resonances in other food samples are shown using existing dielectric data. Resonances in cylinders for both polarizations occurred at similar radii. However, the radius at which the first resonance occurred for the TMz case was absent in the TEz case.
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  • 63
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    AIChE Journal 43 (1997), S. 625-630 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Transient phenomena in solid-liquid fluidized-bed systems are important in designing pulsed, countercurrent (multistage) fluidized-bed contactors of the Cloete-Streat type at high-solids flow rate. Of particular interest are the residence times or corresponding velocities of porosity gradients in the bed and the excess or overshoot height of the bed after refluidization. Theory assuming local equilibrium between holdup and velocity of the phases (local-equilibrium model) for stepwise perturbations in the liquid flow is readily available. It is investigated whether the local-equilibrium theory can be used for more complex perturbations and whether inertia effects, such as are encountered in countercurrent multistage fluidized-bed systems, can be ignored. Therefore, the detailed particle-bed model of Foscolo and Gibilaro, which incorporates inertia effects, was applied to investigate the transient behavior of fluidized-bed systems. Transient fluidization experiments were performed with a broad range of water-fluidized particles in a laboratory-scale multistage fluidized-bed contactor. The operating conditions corresponded to those for countercurrent contact.Numerical simulations with the particle-bed model predict satisfactory experimental results. The “overshoot” heights of the fluidized bed were estimated correctly by the particle-bed model, whereas the local-equilibrium model only provides a conservative estimate. However, the local-equilibrium model allows an analytical solution that is more interesting for design, as it avoids tedious calculations. The residence time of the last perturbation before the fluidized bed relaxes to steady state was estimated with similar accuracy by both models.
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    AIChE Journal 43 (1997), S. 645-654 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A spontaneous, steady capillary flow is produced for a liquid index in a circular tube that is partially coated with a surface modifier to create a discontinuous wetting condition between upstream and downstream portions of the tube. As a means of demonstrating that the flow configuration may prove useful as a diagnostic tool in studies concerning capillarity and the physics associated with the moving contact line, average steady velocities are predicted and compared against a large experimental data set that includes the effects of tube dimensions and fluid properties. To access a wider range of tube diameters (0.516-9.88 mm), experiments are performed employing “U-tubes” tested in the low-gravity environment of a drop tower, in addition to straight capillary tubes tested horizontally in a laboratory. The sensitivity of the steady capillary flow to surface cleanliness is dramatic.
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    AIChE Journal 43 (1997), S. 673-680 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Permeability and compressibility data are required for an adequate process model for compressible-cake filtration and mechanical expression. Experimental and modeling results of the permeability of palm-oil filter cakes (a highly compressible viscoelastic material) are combined with compressibility data, leading to a model for the expression step. Permeability measurements show that permeability depends strongly on the quantity of fine particles in the cake. Removal of fine particles from the slurry before expression significantly increases the solid-phase content during expression due to higher permeability. Modeling results of the expression step show that for palm-oil filter cakes there is a pressure above which the attainable mass fraction of solids becomes independent of pressure. Decrease in specific cake resistance has two effects: a higher mass fraction of solids at the same pressure and a higher pressure at which the mass fraction of solids is not affected further.
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    AIChE Journal 43 (1997), S. 740-753 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Modeling and optimization of a semiregenerative catalytic naphtha reformer has been carried out considering most of its key constituent units. A detailed kinetic scheme involving 35 pseudocomponents connected by a network of 36 reactions in the C5-C10 range was modeled using Hougen-Watson Langmuir-Hinshelwood-type reaction-rate expressions. Deactivation of the catalyst was modeled by including the corresponding equations for coking kinetics. The overall kinetic model was parameterized by bench-marking against industrial plant data using a feed-characterization procedure developed to infer the composition of the chemical species in the feed and reformate from their measured ASTM distillation data. For the initial optimization studies, a constant reactor inlet temperature configuration that would lead to optimum operation over the entire catalyst life cycle was identified. The analysis was extended to study the time-optimal control profiles of decision variables over the run length. In addition, the constant octane case was also studied. The improvement in the objective function achieved in each case was determined. Finally, the sensitivity of the optimal results to uncertainty in reactor-model parameters was evaluated.
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    AIChE Journal 43 (1997), S. 754-760 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Temperature profiles in the continuous-flow reactor with a fixed bed placed in a nonuniform microwave field are predicted by considering specific on - off regulation of the microwave power and local distribution of the electric-field intensity. The spatial and temperature dependence of the electric-field intensity affects the heating appreciably, as shown by indirect measurements. Results of numerical calculations agree with experimental data of outlet temperature and explain temperature oscillations in the outlet flow. The model developed allows an accurate description of microwave heating in the continuous-flow reactor associated with specific regulation of microwave power. The hydrolysis of sucrose catalyzed by the strongly acidic cation-exchange resin Amberlite 200C in R-H form was chosen as the reaction system. Calculated conversions of sucrose based on predicted temperature profiles agree with experimental data.
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    AIChE Journal 43 (1997), S. 777-784 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The oxidative dehydrogenation of butane was studied at temperatures between 450 and 550°C using a conventional fixed-bed reactor and inert membrane catalytic reactors with different feed arrangements. When inert membrane reactors were employed, a ceramic membrane was used to distribute oxygen to a fixed bed of V-Mg-O catalyst. The membrane reactor was found to be more efficient than a fixed-bed reactor under most of the conditions investigated. In addition, in the membrane reactor the formation of hot spots decreased considerably, leading to a more stable and safer operation.
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    AIChE Journal 43 (1997), S. 1691-1699 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pore fractal objects are expected to be optimal catalysts, since material is supplied to the narrower pores, which are also shorter through the larger pores where diffusion resistance is smaller. To demonstrate this, diffusion and reaction were simulated on Sierpinski-gasket-type fractal objects and on the corresponding nonfractal uniform-pore structures of the same size, porosity and reactive area. Positive order reactions limited by Knudsen diffusion were shown to exhibit larger rates in fractal than in uniform-pore objects. Fractal catalysts also exhibited a new intermediate domain in which the rate depends only weakly on the kinetics parameters. In nonmonotomic kinetics the branching point (bifurcation point) was extremely sensitive to the pore structure.
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    AIChE Journal 43 (1997), S. 1709-1716 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An electrochemically modulated complexation (EMC) process was designed to selectively extract and concentrate ethylene from a feed-phase mixture with ethane. Unlike previous EMC processes, where the separation was based on equilibrium solubility differences, this separation was based on differences in the rate of complexing with copper(I) solution between the two gases. The EMC process employed flowthrough electrolysis cells that electrochemically modulate aqueous-phase copper chlorides between high, copper(I), and low, copper(II), affinity states. The liquid phase in the high affinity form contracts a feed gas phase in a hollow-fiber membrane module for selective extraction and contacts the receiving gas phase in the low affinity form for concentration. Experimental results and modeling of the process are reported.
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    AIChE Journal 43 (1997), S. 1751-1762 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A systematic method to synthesize crystallization-distillation hybrid separation processes is presented. Two classes of hybrids are identified for binary mixtures. The first bypasses azeotropes and tangent pinches, while the second bypasses eutectics. Guidelines for flow-sheet selection are proposed based on an analysis of simple eutectic, constant relative volatility systems. In addition, the hybrids are compared to both extractive and adductive crystallization in order to determine the conditions under which solvent-based crystallization techniques outperform the proposed hybrid configurations. The method is extended to ternary mixture. The use of stream combination and complex distillation columns is also considered.
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    AIChE Journal 43 (1997), S. 1819-1827 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In a high-temperature catalytic membrane reactor, a plug-flow reactor is combined with a gas-separative membrane. By selectively removing one of the reaction products, the reaction mixture is prevented from reaching equilibrium, and a higher conversion can be obtained. This concept is only valid for reactions that are limited by the thermodynamic equilibrium, such as the direct dehydrogenation of propane to propene. A tubular H2-selective silica membrane was characterized [αH2/C3H8 = 70-90 at 500°C] and used as the gas-separative membrane. The membrane reactor was filled with a chromia/alumina catalyst. The kinetics of the catalyst was studied. At 500°C the deactivation of the catalyst is slow, and the propene yield is almost constant for at least 10 h of operation. Under well-chosen process conditions, the propene yield is at least twice as high as the value obtained at thermodynamic equilibrium in a conventional reactor.
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    AIChE Journal 43 (1997), S. 1727-1736 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The magnitude and direction of the ultrasonic radiation forces that act on individual particles in a standing-wave field were determined using a microscope-based imaging system. The forces are calculated from measured particle velocities assuming that the drag force, given by Stokes' law, is exactly counterbalanced by the imposed ultrasonic forces. The axial primary radiation force was found to vary sinusoidally with axial position and to be proportional to the local acoustic energy density, as predicted by theory. The magnitude of the transverse primary force was determined by two independent methods to be about 100-fold weaker than the axial force. Separation concepts exploiting the transverse force for cell retention have been successful despite the great disparity in magnitude between the axial and transvers-force components. This may be explained by the reduced hydrodynamic forces on aggregated particles in transverse flow due to their alignment in the sound field.
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    AIChE Journal 43 (1997), S. 1909-1913 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 75
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    AIChE Journal 43 (1997), S. 1894-1903 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The role of swelling clays in hindering the compressional dewatering characteristics of coal-mine tailings is examined. The effects of electrolyte concentration and ion exchange in improving the shear and compressional rheology are compared. Suspensions studies include actual mine tailings (thickener feed and thickener underflow) as well as synthetic clay dispersions made from clay collected from the coal seam. It was shown that the most important parameter in controlling the properties of the tailings suspension is “controlled” dispersion in the presence of a Ca2+ electrolyte concentration in excess of that required to (1) prevent initial swelling and (2) provide full cation exchange of the clay. Under these electrolyte conditions, complete delamination of the clay did not occur, and both the dewatering and handling characteristics of the resultant suspensions improved dramatically.
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  • 76
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    AIChE Journal 43 (1997), S. 2169-2179 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The spread and rebound of droplets upon impact on flat surfaces at room temperature were studied over a wide range of impact velocities (0.5-6 m/s), viscosities (1-100 mPa.s), static contact angles (30-120°), droplet sizes (1.5-3.5 mm), and surface roughnesses using a fast-shutter-speed CCD camera. The maximum spread of a droplet upon impact depended strongly on the liquid viscosity and the impact velocity. The tendency of a droplet to deposit or to rebound is determined primarily by the liquid viscosity and the liquid/substrate static contact angle. A model more broadly applicable than existing models was developed to predict maximum spread as a function of the Reynolds number, the Weber number, and the static contact angle. Based on the conservation of energy, a rebound model is proposed that predicts the tendency to rebound as a function of maximum spread and static contact angle.The maximum-spread model prediction agrees to within 10% with more than 90% of the experimental data from different sources. In the current study, the rebound model successfully predicts the tendency of a droplet to rebound.
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  • 77
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    AIChE Journal 43 (1997), S. 2157-2168 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new concept for removing toxic gases with respirator applications based on inhalation and exhalation through absorbent polymer-lined tubes was studied. The two-way oscillatory flow through the respirator element enables capture and expulsion of toxic vapors, which is not feasible with activated charcoal because of the adverse effect of moist breath on the charcoal efficiency. To study the influence of various material, design and operating parameters on the performance of such a respirator, a numerical model that solves the governing equations of mass and heat transport in an oscillatory flow through an absorbent polymer-lined tube was developed and utilized with experimentally measured property data. Factors investigated include respirator element geometry, polymer and toxic vapor characteristics, and flowthrough speed. Results show a large delay in toxic gas penetration over that obtained by conventional one-way flow, and define the importance of partition coefficient, flow passage size, and length and velocity. Results are compared with data from simulated breathing experiments.
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  • 78
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    AIChE Journal 43 (1997), S. 2215-2226 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simplified exponential (SEXP) approximation, combining the exponential approximation and the first-order mean spherical approximation, is proposed to improve the equation of state for the Lennard-Jones (LJ) fluid. The SEXP approximation, which can be implemented in an analytical manner, yields better radial distribution functions of the LJ fluid. Extensive comparisons with two typical perturbation theories show that the SEXP approximation is more appropriate to describe the behaviors of the LJ fluid. The latest 33-parameter modified Benedict-Webb-Rubin equation, also calculated, is inadequate in the region of phase coexistence. The SEXP approximation is applied to the calculation of methane properties with much better results than the Peng-Robinson equation of state for saturated liquid densities and second virial coefficients.
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  • 79
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    AIChE Journal 43 (1997), S. 2203-2214 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The one-component steady-state permeation of gases through a silicalite-1 zeolite composite membrane as a function of the temperature is studied from 190 to 680 K for light hydrocarbons, noble gases, and some inorganic gases. In general, with increasing temperature the permeance shows a maximum followed by a minimum. For gases weakly adsorbed the permeance has only a minimum and for gases strongly adsorbed only a maximum is observed in the permeance. The permeance for various gases, for a feed pressure of 101 kPa, span four orders of magnitude. The lowest permeation is for i-butane at 300 K: a permeance of 0.07 × 10-8 mol. m-2.s-1.Pa-1. The highest value is observed for methane: a permeance of 70 × 10-8 mol. m-2.s-1.Pa-1 at about 240 K. A comparison between the isobars and the temperature dependence of the steady-state permeance, both at 101 kPa, shows that at the temperature where the amount adsorbed vanishes the permeance starts to increase. The temperature dependence of the steady-state fluxes through the silicalite-1 membrane can be described only if two diffusion mechanisms are taken into account. For high occupancies the mass transport can be described by equilibrium adsorption followed by surface diffusion and for low occupancies the mass transport can be described by activated gaseous diffusion. With increasing temperature the mass-transport mechanism shifts from the surface diffusion regime to the activated gaseous diffusion regime. With these two diffusivities modeling results agree well with experimental results for the one-component flux through the silicalite-1 zeolite membrane.
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  • 80
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    AIChE Journal 43 (1997) 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 81
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    AIChE Journal 43 (1997), S. 2921-2931 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The local structural function obtained by a microresistivity probe at different hydrodynamic regimes is examined. The structures, such as the vortex-clinging structure, the appearance of one and two large cavities, small 3-3 structure, large 3-3 structure, and ragged cavities were recognized by frequency transformation of the time-domain structural function. An optimized phase discrimination in signal processing was used. The distribution of the local void fraction (α) in a pilot-size stirred tank was experimentally investigated, because almost no such data can be found in the literature. The two-phase mixture was composed of air and water; α was measured at 190 nodes in the vertical half-section plane of the vessel. Relative differences smaller than 9% between integrated values of α and measured gas holdups agreed reasonably well under all conditions.
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  • 82
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    AIChE Journal 43 (1997), S. 2279-2288 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Model-predictive control and identification (MPCI) introduced by Genceli and Nikolaou in 1996 is a novel approach to the identification of processes under constrained model-predictive control. MPCI solves an on-line optimization problem that involves (a) a standard MPC objective; (b) standard MPC constraints; and (c) persistent excitation (PE) constraints. The on-line optimization problem is computationally demanding. To alleviate that problem, we take a frequency-domain approach to formulating the PE constraints. This approach relies on the following fact: a signal is persistently exciting of order n, if its two-sided power spectrum is nonzero at no fewer than n points. Therefore, persistently exciting input signals can be parametrized over a finite horizon as a sum of sinusoid terms with nonzero coefficients. Used in the MPCI framework, the last requirement generates a set of completely decoupled reverse-convex constraints that are combinatorially tractable from a computational point of view. The effectiveness of the proposed MPCI method is demonstrated through simulations. For the SISO systems studied, computation of the global optimum could be handled combinatorially in real-time using PC hardware.
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  • 83
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    AIChE Journal 43 (1997), S. 2300-2306 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A novel procedure to find cost-optimal sensor networks is proposed. Cost is minimized subject to qualifying constraints that are related to certain requirements of data reconciliation. One basic qualifying constraint is a desired level of precision of reconciled values for a selected set of variables. Since precision requirements lead to multiple solutions, other qualifying constraints are proposed. These constraints are availability, resilience, and error detectability. Definitions for these terms are given and their impact on the results is presented.
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  • 84
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    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A modified CaCO3 sorbent with an open internal pore structure is prepared and its sulfation characteristics are investigated in an entrained flow reactor at high temperatures (900-1,100°C) and short contact times (20-600 ms) using small particle sizes (〈 5 μm). The most distinguishing feature of this modified carbonate (MC) is its 70-75% sulfation conversion within 0.5 s, which is substantially higher than any other sorbents published. The MC is prepared by carbonation-precipitation from a calcium hydroxide suspension by optimizing the operating parameters to generate carbonate particles of the desired pore structural properties. The high initial surface area combined with its open pore structure and pore-size distribution of its calcine contribute to its high reactivity. The calcined MC possesses a significant portion of its pore volume in the 50-200 Å range. This size range represents an optimum pore size for sulfation since it provides a reasonably high surface area and is less susceptible than 〈 50 Å pore sizes, to pore filling, or pore-mouth plugging due to the formation of higher molar volume CaSO4. Investigation with other carbonates reveals that a much higher portion of their calcines' porosity lies in the smaller pores, which leads to premature termination of sulfation. Results show the impact of internal pore structure on initial reactivity and ultimate sorbent conversion.
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  • 85
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    AIChE Journal 43 (1997), S. 1341-1347 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Previous experimental studies have found that surface interactions significantly affect the transport of motile bacteria through small tubes, along solid surfaces, and through porous media. However, the role that hydrodynamic forces play in the interactions between solid surfaces and motile bacteria remains unclear. In this study, the swimming speeds of populations of Escherichia coli bacteria were measured near (〈 10 μm) and far (〉10 μm) from a flat glass surface at four ranges of orientations to the surface (0°-45°, 45°-90°, 90°-135°, and 135°-180°). Populations of bacteria close to the surface and moving in the orientation range most perpendicular (0-45°) to the surface experienced the greatest change in the swimming speed when compared to the population in the same orientation range located far from the surface. The decrease in swimming speed experienced by this population was on the same order as that predicted by hydrodynamic models of bacterial swimming near surfaces.
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  • 86
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    AIChE Journal 43 (1997), S. 1403-1413 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Industrial design of electrostatic precipitators is based on the transport theory developed by Deutsch (1922), which assumes that transverse turbulent mixing is effective enough to maintain the concentration profile uniform throughout the cross section (i.e., turbulent diffusivity is assumed infinite). To improve understanding of turbulent particle dispersion under the influence of electrostatic forces, a database on particle trajectories was first generated, based on the flow field from a direct numerical simulation of a plate-plate precipitator (Soldati et al., 1993). The effect of various parameters, such as particle size, charge and particle migration velocity, on dispersion and collection efficiency was investigated. Results show that particle concentration profiles are not uniform due to finite values of “turbulent diffusion” coefficient. The simulations indicate that the early stages of particle collection are controlled by particle migration velocity, while final stages are controlled by turbulence diffusion mechanisms.
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  • 87
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    AIChE Journal 43 (1997), S. 1414-1425 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A computed tomographic scanner was developed for imaging gas-holdup distributions in two-phase flow systems such as bubble columns and fluidized beds. The scanner has been used to study the effects of various operating parameters (such as column diameter, superficial gas velocity, and distributor type) on the gas holdup and its distribution in an air-water bubble column. The experimental investigation shows that the column dimensions have no significant effect on the void fraction when the column diameter is greater than 0.15 m. Differences in the holdup distribution due to the kind of distributor used are significant only at low gas flow rates. Surface tension of the liquid has a profound influence on the gas holdup and its distribution.
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  • 88
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    AIChE Journal 43 (1997), S. 2610-2615 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nanosized silicon powders were prepared by gas-phase cluster agglomeration reactions in a low-pressure silane plasma. The formation and agglomeration of clusters leading to the growth of primary particles of powder were studied by in-situ techniques including mass spectroscopy and laser light-scattering experiments. These powders, generally amorphous and crystallized in a reducing atmosphere, were studied in detail by Raman spectroscopy and high-resolution electron microscopy, which revealed a very rough surface of as-prepared single powder particles with structures of 1 to 2 nm. Upon 1-h annealing at temperatures between 300 and 600°C, circular contrast features, 1.5 to 2.5 nm in size, are observed in the amorphous particles, which show medium-range order. A distinct onset of crystallization is observed at 700°C with structures ranging from very small crystalline ordered regions of 2.5-3.5 nm in size to fast-grown multipletwinned crystallites. The crystallization behavior is influenced by the clusters that form primary particles. Observed sintering behavior cannot be explained by a classical approach; hence, theoretical models need to be adapted to nanosized powders.
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  • 89
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    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nanophase SiC/Si3N4 composite powders were synthesized by the carbothermal nitridation of SiO2. These powders have desirable characteristics of high quality with oxygen contents on the order of 1.5 to 2 wt. %, surface area of ∼ 10 m2/g, submicron α-Si3N4, low metallic impurity levels, and a homogeneous distribution of the nanophase SiC phase. High-resolution TEM analysis has shown that the content and size of the nanophase SiC can be varied from 0.5 to 50 wt. % and 25 to 500 nm, respectively, through proper control of raw materials and reactor conditions. To determine how the nanophase SiC reinforcement affects the mechanical properties of Si3N4, densified components were fabricated using both pressureless and pressure-assisted densification methods. TEM analysis revealed that the nanophase SiC particles are distributed both intergranularly and intragranularly throughout the Si3N4, matrix. By controlling the sintering additive package and the sintering conditions, the ratio of inter- to intragranular SiC can be adjusted. Mechanical property measurements at elevated temperatures showed a dramatic improvement in high-temperature strength and creep resistance over components made with commercially available powders.
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  • 90
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    AIChE Journal 43 (1997), S. 2665-2669 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fine particles of dense, high-purity, crystalline BaTiO3were produced by flame-spray pyrolysis. A 0.5-M (Ba:Ti = 1:1) solution of barium acetate, titanium lactate, and water was aerosolized using an ultrasonic generator, and the droplets were delivered into the core of an annular diffusion flame (H2/air) reactor. For all investigated temperatures [∼1,000-∼2,000°C adiabatic], the generated powders were chemically pure, crystalline (primarily tetragonal phase with hexagonal and cubic polymorphs), and unagglomerated. At a low-flame temperature (∼1,000°C ad.), the particles produced were hollow and irregularly shaped. Particles produced at higher flame temperatures (〉∼1,500°C ad.) were dense and homogeneous. Particles showed a transition from a nonspherical porous morphology to a spherical dense morphology with increasing temperature. By increasing residence time, the temperature at which particles became spherical and dense was reduced. Flame-spray pyrolysis provides a useful method for forming dense particles of high melting point materials by aerosol-phase densification.
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  • 91
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    AIChE Journal 43 (1997), S. 2731-2740 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A series of ion-conducting perovskites of the form [La1-xAx] [Co0.2Fe0.8] O3-δ (x = 0.4, 0.6 for A = Sr; × = 0.8 for A = Ba) were investigated for their use as a catalytic membrane for the oxidative coupling of methane (OCM). A-site cations consisting of La0.4Sr0.6 and La0.2Ba0.8 produce materials with the highest oxygen fluxes and result in C2+ selectivities of 50% at 1,098 K, which are significantly higher than those achieved with a powdered catalyst in a packed-bed reactor configuration. Selectivities in these materials appear to be limited by high oxygen ion recombination rates that compete for oxygen with the desired coupling reaction(s). The results also indicate that oxygen fluxes are not limited by diffusion, but by surface exchange rates at the oxygen-lean side of the membrane. The study on stability of these materials showed that all three perovskites could be reduced in a pure methane or ethane stream at 1,023 K, but they were totally stable under reaction conditions where oxygen is present.
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  • 92
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    AIChE Journal 43 (1997), S. 2760-2764 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new selected-area chemical-vapor deposition (CVD) process to fabricate thin sensing films on a micromachined silicon-based, conductometric gas-sensor structure is reported. The CVD method is self-lithographic and does not require the use of masks to define the thin-film region. Since the films are deposited after silicon processing, the selection of film materials is not constrained by their compatibility with the etchants used in silicon micromachining. The presence of conductance electrodes underneath the growing film permits in-situ monitoring of film resistance. The rapid thermal response of the device allows precise termination of film growth once the film resistance has reached a value suitable for gas sensing. The application of this new CVD method is demonstrated for growth of thin platinum films, which are used for sensing of O2 and H2 gases.
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  • 93
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    AIChE Journal 43 (1997), S. 2773-2784 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A quantitative understanding of the thermochemistry of silicate solution systems is necessary for the design, optimization and control of sol-gel, colloid and zeolite preparations. This review is intended to be a compendium of ready information for the growing number of chemical engineers active in solution-based materials synthesis. Experimental measurements of silica solubility, silicate speciation and silanol deprotonation in aqueous solutions are summarized and critiqued. The thermochemical properties of monomeric and dimeric silicate anions are also assessed. The trends that are then evident are compared with expectations from simplistic gas-phase molecular orbital calculations to illustrate the importance of solvation. Finally, the equilibrium behavior of simple silicate oligomers is rationalized, and the future direction to quantitative modeling of the behavior of more complex oligomers is suggested.
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  • 94
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    AIChE Journal 43 (1997), S. 2785-2792 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The fluorescence properties of inorganic and organically-modified rare-earth-doped sol-gel silica are presented. Eu3+-doped ormosils were prepared from Si(OCH3)4 and CH3Si(OCH3)3, (CH3)2Si(OCH3)2, (CH3)2Si(OC2H5)2, or (n-C3H7)Si(OCH3)3 in various proportions. Er3+-doped ormosils were prepared using Si(OC2H5)4 and CH3Si(OC2H5)3, (CH3)2Si(OC2H5)2 or C2H5Si(OC2H5)3. Gels derived from Si(OCH3)4 were also doped with the fluorinated Eu3+ precursors Eu(fod)3, (CF3SO3)3Eu, and (CF3CO2)3Eu·3H2O. The effect of metal ion codopants, which are known to inhibit clustering of Eu3+ in sol-gel silica, on Er3+ fluorescence is also considered. Fluorescence line-narrowing studies of Eu3+-doped samples indicated that significant Eu3 clustering occurs in both the ormosils and fluorinated precursor compositions. Lifetime measurements of the Eu3+-and Er3+ -doped ormosils showed longer lifetimes at low heat treatment temperatures relative to purely inorganic sol-gel silica, but no significant difference remained in fully densified samples. The longest lifetimes were observed for samples doped with the fluorinated precursors, indicating that the fluorinated ligands are effective at reducing the water content in densified gels.
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  • 95
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    AIChE Journal 43 (1997), S. 2802-2808 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zeolite fiber, film, and monolith were prepared by using nanosize titanium silicalite (TS-1) crystals. Films and fibers of TS-1 zeolite were optically transparent, which might be important for advanced optozeolitic materials. A permeation result of gases on monolith indicates that the gas permeation is governed by the Knudsen mechanism. All types of TS-1 zeolites manufactured in this work are observed to be MFI structure with an orthorhombic symmetry. These results suggest that nanosize zeolites have potential to allow morphological design with applications for advanced functional materials.
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  • 96
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    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Active organic molecules are grafted on the xerogel silica matrix by using functionalized alkoxides f-R′-Si(OEt)3, where f is a chemical function and R′ is an alkyl spacer to limit the degrees of freedom of the molecules. Organic-inorganic hybrid films are prepared in which optically nonlinear DRl chromophores are covalently bound to the silica network. These films exhibit a large and stable second-order nonlinear coefficient from second harmonic generation experiments. Protoporphyrin molecules are attached double covalently to the xerogel matrix via flexible organic spacers. Persistent spectral holes are still burnt with moderate fluency at 120 K.
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  • 97
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    AIChE Journal 43 (1997), S. 2832-2836 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Partially polycondensed γ-aminopropylsilanetriol (APSTOL) was used as the silica precursor to prepare magnesium orthosilicate in the form of crystalline forsterite. Aqueous solutions of APSTOL and magnesium acetate were mixed and dried forming a water-soluble product that was converted into forsterite by heating to 1,000°C. The thermal evolution of the system was studied using TGA, FTIR, UV-VIS spectrophotometry, and XRD techniques, especially for the acetate decomposition and transformation of CSiO3-tetrahedra into the orthosilicate anions. The combustion of organics and the crystallization of forsterite proceed at temperatures 350 and 870°C, respectively. At intermediate temperatures, the system contains amorphous silicates with oligomeric and polymeric anions, which gradually depolymerize as temperature increases. The resulting crystalline product contains less impurities (periclase and enstatite) than samples prepared using other wet-chemistry techniques.
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  • 98
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: As a part of fundamental study on the kinetics of electroceramics processing by wet chemical methods, barium titanate precursor gel was prepared using barium acetate and titanium tetra-isopropapoxide as starting materials. Experimental results indicated that barium acetate was distributed uniformly in the gel matrix formed by hydrolysis and polymerization of titanium tetra-isopropoxide. In the subsequent calcination of the gel, rapid formation of barium titanate was observed above 823 K, which resulted from the reaction between titania and barium carbonate or barium oxide(s). The dynamics of barium titanate formation under different heat-up programs was successfully simulated based on a proposed reaction model that incorporates a specified reactive portion of barium acetate in the gel matrix.
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    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 2878-2888 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Porous ceramics were fabricated by firing ceramic-polymer composites prepared by casting dispersions containing cellulose acetate, water and colloidal ceramic particles (alumina and silica). Composite microstructures were determined by polymer phase separation. Polymer adsorption on the ceramic particles was necessary for the formation of controlled porosity in the composite and fired ceramic. Cellulose acetate adsorbed on alumina, but not silica particles. The microstructure of alumina-cellulose acetate composites was characterized by unagglomerrated alumina particles in a polymer matrix; relatively large pores (pore diameter ∼ 15 μm) formed when the alumina concentration was low (〈50 vol. % relative to the polymer in the dried composite), and smaller pores (pore diameter ∼2μm) developed at higher alumina contents. By contrast, silica-cellulose acetate composites contained agglomerated silica particles and a large pore structure invariant to silica content. Firing of alumina-cellulose acetate composites with ceramic contents greater than 40 vol. % resulted in crack-free porous ceramics with pore structures comparable to the original composites.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 2970-2983 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A Sorbex-type SMB separation instrument was operated for the separation of mixtures of 2-phenyl ethanol and 3-phenyl-l-propanol on a series of eight columns packed with Zorbax C18 bonded silica, using a 60:40 (u/v) solution of methanol and water as the mobile phase. The experiments were carried out with low-concentration mixtures under linear conditions. The elution profiles of both compounds between any two successive columns of the set under steady-state conditions were recorded while compositions of the raffinate and extract were measured. The four safety margin factors, βj, were divided into two groups: β1 and β4; β2 and β3 with different influence. Within the framework of the linear, ideal model of chromatography, a range of flow rates enables an SMB to operate successfully by completely separating the feed into its two components. The influence of different flow rates on the performance of the separator was studied by calculations and experiments with excellent agreement between both sets. With a simple optimization strategy, the four flow rates are selected to maximize the production rate or the concentration of extract and raffinate products, or to minimize the desorbent consumption. These optimum conditions are obtained by controlling the locations of concentration profiles inside the SMB. A reliable model for SMB operation allows a rapid and easy selection of the optimum experimental conditions.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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