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  • 1970-1974  (27,283)
  • 1965-1969  (23,900)
  • 1955-1959  (13,362)
  • Chemistry  (64,374)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 16 (1974), S. 145-152 
    ISSN: 1432-0827
    Keywords: EHDP ; Bone ; Chemistry ; Serum ; Rabbits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The effects of disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) on bone and serum chemistry were investigated in adult rabbits. EHDP was administered by subcutaneous injection at doses of 0.25, 2.5 and 10 mg/kg body weight/day for of 28 days. Blood samples were obtained weekly from each rabbit and serum levels of total calcium, ionized calcium, inorganic phosphate and alkaline phosphatase were determined. At the end of the treatment period all rabbits were sacrificed and the tibiae removed for chemical analysis and histological evaluation. The effect of EHDP administration on serum chemistry was both dose- and time-related. The highest of the three doses, 10 mg/kg/day, resulted in a time-related decrease in total serum calcium. This dose also caused a rapid but transient reduction in serum ionized calcium. The effect of EHDP on serum inorganic phosphate was biphasic. Administration of 2.5 mg/kg/day resulted in a time-related elevation in this parameter, whereas the 10 mg/kg/day dose resulted in a time-related hypophosphatemic response. There were no significant drug-related changes in tibial fat-free dry weight, ash weight, total calcium or total phosphorus values. However, administration of 2.5 and 10 mg/kg/day EHDP resulted in increased osteoid tissue as measured histologically. These results are compared with data from other EHDP studies, and discussed in relation to the maturity and growth-state of the experimental animals.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 143 (1974), S. 115-126 
    ISSN: 1432-0568
    Keywords: Cerebral cortex ; Apical dendrites ; Dendritic bundles ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In frontal, horizontal and sagittal paraffin sections through the cat's brain regional differences in the pattern of dendritic bundling have been investigated. It is shown that in the gyrus splenialis and suprasplenialis 2–5 apical dendrites of layer IV pyramids approach each other to form vertical bundles which run straight through layer IV. In the posterior sigmoid gyrus, on the other hand, most apical dendrites arising from the pyramidal cells bifurcate immediately above layer V and give rise to obliquely running branches which cross each other before turning upwards and joining other dendrites to take part in the formation of bundles. In this region the dendritic tree of the pyramidal cells seems to be characterized by an unexpectedly high degree of directional specialization.—In functional terms the findings can be summarized by stating that in the visual and sensorimotor regions of the cat's cerebral cortex basic differences exist in the relation between the apical dendrites of layer V pyramids.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 143 (1974), S. 127-134 
    ISSN: 1432-0568
    Keywords: Reticular formation ; Crossed connections ; Autoradiography ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Injection of radioactive leucine in various regions of the brain stem reticular formation has revealed the presence of ample crossed reticulo-reticular connections in the cat. The terminal area for the crossed fibers are almost mirror images of the injected sites. The findings made is another example that hitherto unknown fiber connections can be demonstrated by axoplasmic protein tracing.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 146 (1974), S. 1-20 
    ISSN: 1432-0568
    Keywords: Placenta ; Cat ; Differentiation ; Function ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'ultrastructure du labyrinthe de placenta de chatte a été étudiée du 45e au 63e jour de gestation. Cette formation endothéliochoriale est composée: d'un endothélium maternel hypertrophié et de cellules géantes déciduales; d'une couche de «substance inerte interstitielle»; du trophoblaste constitué d'une assise syncytiale continue (syncytiotrophoblaste) et d'une assise cellulaire discontinue (cytotrophoblaste); d'un endothélium foetal accompagné de son environnement mésenchymateux. Les capillaires foetaux envahissent progressivement le syncytiotrophoblaste. L'hypertrophie de l'endothélium maternel diminue en fin de gestation; de ce fait la distance, séparant le sang maternel du sang foetal, peut atteindre en certains points 2 μ. L'endothélium maternel, le syncytiotrophoblaste et l'endothélium foetal présentent des signes ultrastructuraux d'un rôle de transfert. La «substance inerte inerte interstitielle», qui sépare les tissus d'origine maternelle des tissus d'origine foetale, est constante mais d'épaisseur variable; elle est spécifique des structures endothéliochoriales. Les cellules géantes déciduales, dont le nombre diminue en fin de gestation comportent parfois un ou plusieurs «corps glycogéniques». Le syncytiotrophoblaste, siége d'activité de biosynthése de stéroïdes, est riche en ergastoplasme, ce qui autorise à postuler la synthése d'hormones protéiques.
    Notes: Summary The ultrastructure of feline placental labyrinth has been studied from the 45th to the 63rd day of gestation. The endotheliochorial structure is composed of: a thick hypertrophied maternal endothelium with giant decidual cells; an “interstitial inert substance”; a continuous syncytial layer (syncytiotrophoblast) and a discontinuous cellular layer (cytotrophoblast) of trophoblast; and a thin foetal endothelium with its surrounding mesenchyme. Foetal capillaries increasingly invade the syncytiotrophoblast. Maternal endothelial hypertrophy is reduced in the last days of gestation. Thus the interval between maternal and foetal bloods may in some areas become 2 μ, in late pregnancy. Foetal endothelium, syncytiotrophoblast, and maternal endothelium demonstrate ultrastructural features of transfer function. The “interstitial inert substance”, which separates maternal from foetal tissue was always evident though with variable thickness. The giant decidual cells which are reduced in late pregnancy, show one or several “glycogen bodies”. The syncytiotrophoblast, where some steroïd biosynthesis has been demonstrated, has an extensive rough endoplasmic reticulum suggestive of protein hormone synthesis activity.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 146 (1974), S. 141-156 
    ISSN: 1432-0568
    Keywords: Tapetum lucidum cellulosum ; Cat ; Development ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die postnatale Entwicklung des Tapetum lucidum cellulosum der Katze wird mit licht- und elektronenmikroskopischen Methoden untersucht. Bereits am ersten postnatalen Tag sind im Bereich des prospektiven Tapetum zwei Zellarten voneinander zu unterscheiden: 1. mesenchymale Bindegewebszellen und 2. prospektive Tapetumzellen, die durch elektronendichte Tapetumstäbchen gekennzeichnet sind. Die Mesenchymzellen unterteilen als parallel zur Retinaoberfläche ausgebreitete Zellplatten in der Choriodea am hinteren Augenpol den weiten extracellulären Raum in 20–25 etwa 5 μm hohe Schichten. Die Tapetumzellen liegen zwischen den Mesenchymzellplatten und wachsen im Verlaufe der ersten vier postnatalen Wochen innerhalb der Schichten in die Breite, bis sie den extracellulären Raum vollständig ausfüllen und als polygonale Zellen direkt aneinander grenzen. Im weiteren Verlauf der Entwicklung werden die Mesenchymzellplatten rückgebildet, so daß bei der adulten Katze die Tapetumzellschichten direkt übereinander liegen und nur von Netzen elastischer und kollagener Fasern getrennt sind. Die von einer Elementarmembran umgebenen Tapetumstäbchen enthalten einen elektronendichten, in den ersten postnatalen Wochen mit einer Periode von 100 Å quergestreiften Kern. Zunächst nehmen sie an Zahl und Länge zu und füllen am Ende der vierten postnatalen Woche, zu Bündeln von parallel verlaufenden Stäbchen geordnet, das Cytoplasma der Tapetumzellen. Dann nehmen die Tapetumstäbchen an Dicke zu, und ihre Querstreifung wird von einem elektronendichten Material überlagert. Die Entwicklung der Tapetumstäbchen hat eine starke Ähnlichkeit mit der in der Literatur beschriebenen Entwicklung von Melanosomen in Melanocyten. Das Tapetum lucidum cellulosum wird als ein dichter Verband hochdifferenzierter extrakutaner Melanocyten angesehen.
    Notes: Summary The postnatal development of the tapetum lucidum cellulosum of the cat was studied by light and electron microscopy. Already by the first postnatal day two cell types can be distinguished in the prospective tapeta area: 1. mesenchymal cells and 2. prospective tapetal cells, characterized by electron dense, membrane bound, rod-like inclusions. The flattened mesenchymal elements form 20–25 separate layers of cells, which are arranged parallel to the surface of the retina, subdividing the extracellular space of the chorioidea at the posterior pole of the eye into 5 μm high compartments. These compartments contain the tapetal cells which enlarge (in their longitudinal axis) during the first four weeks post partum until they occupy the extracellular space almost completely. At this stage, the tapetal cells are polygonal in shape and closely attached to each other. During the subsequent period of development there is a gradual involution of the mesenchymal cell plates. Thus, in adult cats the individual layers of tapetal cells are only separated from each other by networks of collagen and elastic fibers. The tapetal rods are bound by unit membranes and contain an electron dense core which, during the early postnatal weeks, exhibits a periodic cross-striation (100 Å). The tapetal rods increase in number and length during the first four weeks post partum; by the end of the fourth week, they occupy the whole cytoplasm of the tapetal cells. Parallelly arranged rods are grouped into individual bundles coursing inside the cytoplasm in different directions. Thereafter, the tapetal rods increase in thickness and their cross-striation becomes obscured by an electron dense material. This development of the tapetal rods closely resembles that of melanosomes. Thus the tapetum lucidum cellulosum can be regarded as a compact tissue made up of modified extracutaneous melanocytes.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 14 (1974), S. 3-14 
    ISSN: 1432-0827
    Keywords: Hydroxyapatite ; Mineral ; Phase ; Chemistry ; Synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des diagrammes de phase d'équilibre ont été déterminés pour le système CaO-P2O5-H2O en utilisant des techniques de synthèse hydrothermique au cours de variatio nsde température allant de 300–600° et 2 Kb H2O de pression. De l'hydroxyapatite bien cristallisé a été synthétisé et caractérisé. De faibles variations de paramètres de la maille cristalline, liées à la température de synthèse et composition globale du matériel initial, ont été déterminées. Des conditions chimiques précises sont nécessaires pour obtenir de l'apatite, en tant que seule phase solide en équilibre dans la solution. Les résultats de diagramme de phase d'équilibre sont comparés avec ceux obtenus dans des milieux synthétiques.
    Abstract: Zusammenfassung Es wurden Gleichgewichts-Phasendiagramme für das System CaO-P2O5-H2O bestimmt, indem hydrothermale Synthese-Techniken im Temperaturbereich von 300–600° und bei einem Druck von 2 Kb H2O verwendet wurden. Es wurde gut-kristallisiertes Hydroxyapatit erzeugt und charakterisiert. Es wurden geringe Unterschiede in den Parametern der Zelleinheiten festgestellt, welche von der angewandten Temperatur und der Zusammensetzung des Startmaterials abhingen. Es waren genaue chemische Bedingungen nötig, um Apatit als die einzige feste Phase im Gleichgewicht mit der Lösung zu erhalten. Die Resultate der Gleichgewichts-Phasendiagramme werden mit früheren Untersuchungen mit der Synthesetechnik verglichen.
    Notes: Abstract Equilibrium phase diagrams have been determined for the system CaO-P2O5-H2 using hydrothermal synthesis techniques in the temperature range 300–600° and 2 Kb H2O pressure. Well-crystallized hydroxyapatite has been produced and characterized. Small variations in unit cell parameters dependent on temperature of synthesis and bulk composition of the starting materials have been determined. Precise chemical conditions were required to obtain apatite as the only solid phase in equilibrium with solution. Equilibrium phase diagram results are compared with previous synthetic investigations.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 19 (1974), S. 507-521 
    ISSN: 1432-1106
    Keywords: Cat ; LGN ; Transients ; Antagonistic inhibition ; Sensory storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Neuronal responses to interruptions of light stimuli were studied in 58 X-type and 7 Y-type LGN relay cells with intra- and extracellular recording techniques. The responses to interruption were then compared with responses to either appearing or disappearing light stimuli which had the same luminance and size as the interrupted stimulus. The extent to which responses to interruption differed from those to appearance and disappearance was studied as a function of the interstimulus interval (ISI), the duration of the stimulus before the interruption (t1) and after the interruption (t2). Responses to stimuli appearing after interruptions of up to 500 msec were weaker than those to appearance and disappearance. This difference decreased with increasing ISI. The difference between responses to appearance and interruption increased as t2 decreased, and decreased as t1 decreased. The reverse was true for the difference between responses to disappearance and interruption. Stimulation of the mesencephalic reticular formation consistently reduced the difference between responses to appearance and disappearance compared with those to interruption for all stimulus conditions. These results are discussed in the context of the psychophysical study presented in the preceding paper. It is shown that the neuronal reactions are reflected in detail by the ability of humans to detect appearances and disappearances that occur during interruptions. EPSP sequences recorded from LGN relay cells and relay cell responses conditioned by reticular stimulation suggest that the differentiation of responses to interruption and change partially occurs already in the retina. This differentiation is subsequently enhanced by antagonistic inhibition in the LGN. It is proposed that these inhibitory interactions at peripheral sites of the visual pathway store visual information and serve to reduce the redundancy of responses to short interruptions of the visual input. Centrifugal modulation of LGN inhibition is proposed to control the trade off between temporal integration and temporal resolution.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 20 (1974), S. 125-134 
    ISSN: 1432-1106
    Keywords: Somato-sympathetic reflexes ; Cutaneous and muscle postganglionic neurones ; Noxious stimulation of skin ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Somomato-sympathetic reflexes in postganglionic neurones to skin and muscle induced by noxious stimulation of skin were studied in cats anaesthetized with chloralose. The reflexes were elicited by mechanical damaging stimuli and by temperature stimuli of more than 45°C. 2. In most cutaneous units the spontaneous activity was depressed during noxious stimulation of skin. Some cutaneous units were excited or showed mixed responses. The depression of the spontaneous activity was maximal from the skin area which was innervated by the cutaneous postganglionic neurones and much weaker or not elicitable from other skin areas. 3. Most muscle units were excited during noxious stimulation of skin. This excitation could be elicited from all over the body surface. 4. The effective cutaneous afferent fibres which are involved in these reflexes are the Group III axones which are excitable by mechanical damaging stimuli and the Group IV axones which are excitable by mechanical damaging and/or by thermal noxious stimuli. 5. These investigations reveal that the somato-sympathetic reflexes have opposite organization in cutaneous and muscle postganglionic fibres. The reflex patterns are discussed in relation to a possible central organization of the sympathetic nervous system.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 20 (1974), S. 297-302 
    ISSN: 1432-1106
    Keywords: Cat ; Selective rearing ; Maturation ; OKN ; Perception of motion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Kittens reared in the dark from birth were exposed one hour a day between the 4th and the 10th week of age, to a visual environment which consisted exclusively of vertical edges moving horizontally at a constant speed, and always in the same direction. Total exposure time varied between 10 and 60 hours. 2. At the 12th week of age, optokinetic nystagmus (OKN) in response to displacements of the visual field, was tested. Displacements in the direction that the kittens had experienced during the exposure period elicited immediately an OKN, the frequency of which was related to the speed of the moving pattern. Displacements in the opposite direction elicited a poorer response, only for slowly moving edges. When the speed of the displacement was increased, OKN failed to adapt and finally disappeared. 3. These results suggest the existence, in this type of visuomotor behavior, of a component built up by early visual experience (adaptative component) overlying another component pre-existing visual experience (pre-programmed component).
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 21 (1974), S. 67-82 
    ISSN: 1432-1106
    Keywords: Sensory transmission ; Lateral geniculate nucleus ; Visual cortex ; Attentive behaviour ; Vigilance ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The behaviour of 6 cats was studied in a vigilance task. Each cat was trained to press a pedal on the floor of a training box. A waiting interval of fixed (FI) or variable (VI) duration then followed, after which a stimulus (S), a spot of light or a tone, was presented for a short period of time. The cat gave a correct response if, during this time, it pressed a panel. The animal was then rewarded with food. When an experiment had been completed using one S the cat was trained to respond to the other. The latency of response following the onset of S was measured for each waiting interval in the VI schedules. It was found that the longer latencies were associated with the shorter waiting intervals; that is, the cats responded more quickly to S as expectancy increased. Between the time the pedal and pand were pressed the optic tract (OT) was shocked not more than once and the response of the LGN and visual cortex recorded. The time at which the shock was delivered varied from 1 trial to the next. The responsiveness of the LGN and visual cortex did not vary during the waiting interval in either of the FI schedules or in the VI schedule in which S was a spot of light. However, in the VI schedule in which S was a tone, the responsiveness of the visual cortex to the thalamocortical input declined as the length of the waiting interval increased. No changes were observed at the LGN or in the presynaptic cortical response. These results contrast with those observed during changes in the level of arousal as assessed by the ECoG. When the ECoG passed from the synchronised to the desynchronised state there was an increase in the amplitude of the postsynaptic LGN response to the OT shock, but no change in the responsiveness of the cortex to the increased thalamocortical input. These changes in transmission in the visual pathways are discussed in relationship to the animal's behaviour and to the inferred state of attention.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 21 (1974), S. 139-154 
    ISSN: 1432-1106
    Keywords: Electrotonic coupling ; Antidromic responses ; Abducens motoneurone ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The electrical stimulation of the abducens nerve provokes the classical true antidromic invasion of the abducens motoneurone and a depolarization which is often capable of generating full action potentials in the impaled motoneurone. Experiments studying these depolarizations suggest the existence of electrotonic coupling between the abducens motoneurones of the cat. Intracellular activity of the abducens motoneurones was recorded following intracellular stimulation of the impaled neurone and antidromic stimulation of the abducens nerve. Collision between the outgoing action potential and the antidromic volley differentiated the true antidromic spike from the depolarizations which can induce or not action potentials. The latency of the depolarization ranged between 100 and 1200 μsec. Collision demonstrated that the depolarization and the true antidromic action potential have an independent origin. The depolarizations and action potentials which are not conveyed to the motoneurone by its own axon are interpreted to be generated by electrotonic coupling.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 21 (1974), S. 473-486 
    ISSN: 1432-1106
    Keywords: Lateral reticular nucleus ; Reticulocerebellar projection ; Cerebellum ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The areas of the cerebellar cortex receiving fibres from the mLRN (major portion of the lateral reticular nucleus comprising its parvi- and magnocellular parts) were determined by identifying the low-threshold spots on the cerebellar surface from which 286 mLRN neurones could be antidromically activated. 2. The mLRN fibres terminate bilaterally in the anterior lobe and pyramis, and ipsilaterally in the paramedian lobule. Some fibres terminate also in the rostral part of lobule VI but hardly any in other parts of the cortex. It follows that the mLRN projects almost exclusively to the classical spinal receiving areas. 3. Neurones in the parvi- and magnocellular parts project to similar cortical areas. 4. Two regions in the mLRN were distinguished on the basis of their different projection areas. Region A, a dorsolateral portion of the magnocellular part, projects to the ipsilateral parts intermedia of the anterior lobe and the ipsilateral paramedian lobule. Region B, a ventral portion of the parvi- and magnocellular parts, projects bilaterally to the pars intermedia and vermis of the anterior lobe and sparsely to the pyramis. 5. Neurones throughout the parvi- and magnocellular parts receive monosynaptic excitation from fibres ascending in the ventral part of the ipsilateral lateral funiculus.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 21 (1974), S. 315-336 
    ISSN: 1432-1106
    Keywords: Visual cortex (area 17) ; Cat ; Columnar organization ; Retino-cortical scatter ; Intracortical connections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Responses of cortical cells in the cat's area 17 (central and paracentral area), recorded successively during electrode penetrations perpendicular to the surface, were averaged (PSTH). All cells recorded during one penetration were stimulated with the same stimulus, a slowly moving light or dark slit oriented optimally for the first cell recorded. Comparisons between successively recorded cells were completed by simultaneous recordings from two neurones with the same microelectrode tip. Eye movements as an error were excluded by simultaneous recording of a geniculate cell throughout a cortical penetration. 2. The centers of excitatory receptive fields (ERFs) of simultaneously or successively recorded cells during a penetration may be separated by more than 4°. The mean scatter around a column average is 0.81±0.99° in both directions. The scatter is independent of the recording depth. Whereas the optimal orientation of cells recorded during one penetration was generally similar, the optimal direction (forward and backward movement of an optimally oriented stimulus) was variable. 3. The ERF diameters as determined from the PSTH were between 〈0.5° and 7.5°. During each penetration, cells with small (up to 3.0°) and large (〉3.0°) ERFs could be discriminated. The inhibitory fields (determined with the conditioning method of Bishop, Coombs and Henry, 1971) were between 2.0 and 8.5° along both the optimal and the non-optimal orientation axis of a cell. The borders of inhibitory fields of cells collected during one penetration were also scattered though overlapping. 4. Response analysis of simultaneously and successively recorded cells with different stimuli indicated that, in spite of considerable ERF-overlap, cells with small ERFs had separate excitatory inputs and that intracortical excitatory connections between cells recorded during one penetration were improbable. 5. The ERFs of cells with large ERFs covered a field approximately corresponding to the fields of cells with small ERFs. But a convergent input from many small ERF cells to single large ERF cells was excluded because of the incompatible functional properties of both types of cells, which correspond to some extent to simple and complex cells respectively. 6. It is concluded that cells within cortical cylinders are not connected through excitatory contacts with each other and that most cells in area 17 are excited by individual excitatory geniculate or cortical inputs. Inhibitory connections seem to be the most important intracortical connections. 7. In an Appendix it is shown that anatomical and physiological data do not support significant excitatory convergence of specific geniculate afferents on cortical neurones.
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  • 14
    ISSN: 1432-1106
    Keywords: Reticular formation ; Superior colliculus ; Cat ; Experimental anatomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Following altogether 18 stereotactically placed lesions of different location and size in the superior colliculus the efferent fibres to the RF and their distribution were traced in silver impregnated, approximately serial sections (Nauta and Fink and Heimer methods), cut in the transverse, horizontal or sagittal plane. The projection to the mesencephalic RF was found to be almost completely ipsilateral, that to the pontomedullary RF largely contralateral. In the mesencephalic RF the fibres end in its dorsal half approximately. In the pons and medulla they supply only the medial 2/3 of the main RF, with two distinct maxima within the total field of termination. One maximum covers the rostral part of the nucleus reticularis gigantocellularis and the adjoining part of the nucleus reticularis pontis caudalis, the other occupies the rostralmost part of the latter and the caudal part of the nucleus reticularis pontis oralis. Of the precerebellar reticular nuclei the contralateral nucleus reticularis tegmenti pontis receives a distinct component of tectal fibres in a small area dorsomedially. Some fibres end in a restricted part of the nucleus reticularis lateralis and in the paramedian reticular nucleus. The tectoreticular projection appears to be organized according to the same principles as other afferents to the RF. In the main RF the areas of termination of the tectoreticular fibres coincide more or less with the areas of termination of corticoreticular, fastigioreticular and vestibuloreticular fibres. These common terminal areas are those which give off the bulk of reticulospinal fibres. Some functional implications of the pattern of organization in the tectoreticular projection are discussed.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 19 (1974), S. 467-477 
    ISSN: 1432-1106
    Keywords: Suprasylvian gyrus ; Cat ; Single neurons ; Eye movements ; Direction sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 270 single neurons from the anterior part of the middle suprasylvian gyrus (AMSS) were recorded in awake and non-paralyzed cats (Chronic preparation). 10% were unresponsive to visual stimulation, the remainder reacted well to moving visual stimuli. Half of the units tested were directionally selective. Horizontal, or downward preferred directions predominated. Most neurons were relative insensitive to changes of shape, orientation, contrast, and velocity of the visual stimulus. Some neurons preferred rapid (100°/sec) jerky movements, others required complex motions of irregular shapes, a few strongly preferred objects moving towards the animal in the midsagittal plane. 40% of neurons yielded phasic On-Off reaction to flashing stationary spots. Habituation to repeated stimulation was a common feature and occured in 50% of AMSS neurons. In 19% of neurons tested the discharge rate was not affected by saccadic eye movements, when the animal faced a patterned background. Among the remainder two types of saccade associated responses could be distinguished. Type I discharged prior to or simultaneously with the onset of saccades. This early response was usually associated with saccades of particular directions. Saccades in total darkness yielded weaker and less consistent responses. Type II discharged subsequent to the onset of the saccades after a latency of 40 msec (type IIa), 40–80 msec (type IIb) and 80 msec (type IIc). Responses of type IIa are probably consequences of the retinal effects of eye movements. The saccade associated responses of type Ia, IIb and IIc are tentatively interpreted as results of an eye movement-synchroneous subcortical input, which facilitates transmission in AMSS neurons. Presaccadic facilitation, which generates type Ia responses, may be functionally related to shifts of attention prior to eye movements. It is suggested that postsaccadic facilitation, which underlies the reactions of type IIb and IIc, may be a correlate of visual attention during the fixation period.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 19 (1974), S. 36-60 
    ISSN: 1432-1106
    Keywords: Cerebellar nuclei ; Motor cortex ; Evoked potentials ; Topographical organization ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. A topographical study of cerebello-cortical relations was conducted in cats anesthetized with chloralose. The potentials evoked by punctate stimulation of the cerebellar nuclei were recorded at various sites in the contralateral motor cortex. A map of the cortical responses obtained by stimulating each of the cerebellar nuclei was established. For this purpose, the stimulation thresholds as well as the response amplitudes, polarity and latency were noted. 2. Local stimulation of each cerebellar nucleus evoked responses in particular regions of the contralateral motor cortex. When a stimulus was applied to the interpositus nucleus, short latency responses were recorded in the lateral part of the area 4. Dentate nucleus stimulation induced short latency responses in the medial part of area 4 and in area 6. Furthermore, late responses were also recorded laterally in area 4. 3. The interpositus nucleus was found to project to the portion of motor cortical area controlling the distal forelimb with only a fast conducting pathway. The fastigial nucleus seems to be connected with proximal and axial motor areas. The projection system of the dentate nucleus appears to have a temporal organization: a fast conducting pathway was found to project to the axial and proximal motor area while a slower one seems to transmit impulses to the motor area of the forelimb extremity. 4. The functional aspects suggested by the temporal organization of the cerebellocortical inputs and the distribution of each nucleus projections on the motor cortical areas are discussed.
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  • 17
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    Experimental brain research 20 (1974), S. 101-114 
    ISSN: 1432-1106
    Keywords: Size principle ; Motoneurons ; Cat ; Recruitment ; Neurophysiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Henneman et al. have shown that a wide variety of stimuli activate motoneurons in the order of their sizes, though some reversals occur. It is not clear which motoneurons should be included in the “pool” from which recruitment by size takes place. The “pool” may consist of 1. all the motoneurons to a given muscle 2. all the motoneurons in a given ventral root or 3. all the motoneurons activated by a given stimulus. We have examined recruitment in the pool of fibers to a given muscle. A variety of mechanical stimuli to cat's legs were used to elicit bursts of activity in gastrocnemius motor units. In 30% of pairs of units, one unit had a consistently lower threshold, i.e. it always began and ended a burst. In the other 70%, either unit could start or end a burst. For 48% of motor unit pairs recorded in either the medial or lateral gastrocnemius, each unit of the pair had runs of firing of at least 10 spikes while the other was totally inactive. By the same criterion, 23% of pairs of temporalis units showed differential activity. During spontaneous breathing there was no consistent recruitment order in 24% of pairs of units in throat respiratory muscles. These data indicate that reversals of recruitment order are relatively common for motoneurons innervating the same muscle. We discuss the possibility that size principle may hold for motoneurons in a single ventral rootlet whose somas are anatomical neighbors (even though they innervate different muscles), but not for motoneurons that are anatomically distant in the cord (even though they innervate the same muscle).
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  • 18
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    Experimental brain research 20 (1974), S. 135-143 
    ISSN: 1432-1106
    Keywords: Cat ; Renshaw cell ; Antidromic activation ; Differential blocking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Attempts were made to study differences in the relative effectiveness of different size ranges of motor axons to Renshaw cells by differential blocking of larger fibers of the gastrocnemius nerve in cats anesthetized with Nembutal. 1. Differential blocking of larger fibers in the nerve was successfully obtained by applying trapezoid wave current to the nerve. 2. It was shown that more than half (58.1%) of the Renshaw cells receive homogeneous inputs from a motor axon collaterals, 25.8% of the cell receive collateral inputs from a certain group of fibers, and 12.5% of the Renshaw cells were activated by “γ range” fibers.
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  • 19
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    Experimental brain research 20 (1974), S. 115-123 
    ISSN: 1432-1106
    Keywords: Somato-sympathetic reflexes ; Postganglionic fibres to skin and muscle ; Natural stimulation of skin ; Hair-follicle receptors ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Somato-sympathetic reflexes in postganglionic neurones to hairy skin and to muscle produced by mechanical non-noxious stimulation of skin were studied in cats anesthetized with chloralose. Most of the postganglionic fibres investigated were spontaneously active and had presumably vasoconstrictor function. 2. In 60% of the cutaneous postganglionic neurones stimulation of hairs induced predominantly excitation. This excitation was mostly followed by a slight depression of the spontaneous activity. In 30% of the neurones the spontaneous activity was depressed or predominantly depressed by these stimuli. 3. In most muscle postganglionic neurones the spontaneous activity was depressed by stimulation of hairs. 4. In both types of neurones the reflexes were produced by activity in hair follicle receptors with Group II afferents. Hair follicle receptors with Group III afferents most probably also contributed to this effect. Except for a slight depression of the spontaneous activity in some cutaneous postganglionic neurones by slowly adapting receptors, mechanical stimulation of other types of receptors with Group II afferents had no effect on the postganglionic neurones. 5. Reflexes in postganglionic neurones could be elicited by stimulation of hairy skin all over the body surfaces.
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  • 20
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    Experimental brain research 20 (1974), S. 273-283 
    ISSN: 1432-1106
    Keywords: Vestibular nuclei ; Thalamic projections ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Electrolytic lesions were placed into the left vestibular nuclei of 22 adult cats. Silver impregnation for degenerated terminal fibres of serial sections revealed the existence of a relay of the corticopetal vestibular pathway in the contralateral thalamus. Its main afferent fibres originate in the superior and medial vestibular nuclei. By crosschecking the results it was possible to demarcate a focus of vestibular projections in the ventro-caudal part of the thalamus, situated between the centre median, the ventro-basal group and the caudal segment of the ventro-lateral nucleus. The direct ascending afferents of this focus are relatively few in number. The functional significance of this vestibular relay with regard to the proprioceptive influx passing through this region of the thalamus is open to discussion.
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  • 21
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    Experimental brain research 20 (1974), S. 285-296 
    ISSN: 1432-1106
    Keywords: Ampullary nerve ; Cat ; EPSP ; IPSP ; Vestibular neuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The six ampullary nerves in both labyrinths were separately stimulated by electric pulses in anesthetized cats. Resulting responses in neurons in the vestibular nuclei were recorded intracellularly. Almost all the vestibular neurons showed an EPSP in response to stimulation of the ipsilateral ampullary nerve. These neurons were subclassified as A-, L-, and P-neuron receiving specific projections from the anterior, lateral and posterior canal, respectively. Three fourths of the vestibular neurons recorded from received an IPSP in response to stimulation of the contralateral ampullary nerve. Plane-specific contralateral inhibition was found in most of vestibular neurons; i.e. A-, L-, and P-neuron received IPSP from the contralateral posterior, lateral, and anterior ampullary nerve, respectively. Approximately two thirds of vestibular neurons exhibiting the plane-specific inhibition were recorded in the medial vestibular nucleus. A collision test of impulses in primary afferent fibers were performed during recording of ipsilateral EPSPs produced by strong stimulation of more than one ampullary nerve. No positive evidence was provided for the existence of neural convergence on single vestibular neurons from different ampullary nerves on the same side. It is suggested that the plane-specific contralateral inhibition increase the sensitivity of vestibular neurons during head rotation.
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  • 22
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    Experimental brain research 20 (1974), S. 347-362 
    ISSN: 1432-1106
    Keywords: Vestibular nuclei ; Tilt response ; Sinusoid ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Extracellular recordings were made in the lateral and inferior vestibular nuclei of decerebrate, unanesthetized cats. The firing patterns of single units were studied using small amplitude sinusoidal roll tilts of from 0.01 Hz to 1.0 Hz. Three-fourths of the tilt-sensitive units showed greater modulation of their firing rates as the frequency of the sinusoidal tilt was increased. The responses of cells in both nuclei were similar. These responses were virtually unchanged in cats with chronically plugged semicircular canals, indicating a probable otolith origin for the dynamics of the tilt response.
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  • 23
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    Experimental brain research 20 (1974), S. 363-374 
    ISSN: 1432-1106
    Keywords: Pain ; Somatosensation ; Cerebral cortex ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The experiment examines the role of the cerebral cortex in regulating responses to noxious stimulation by studying the effects of cortical ablations in cats on escape from electric shock applied to the foot pads. The latency of escape as a function of shock intensity was measured before and after bilateral ablations of various portions of the cat's somatosensory cerebral cortex. Ablations of the second somatosensory cerebral cortex significantly increased the escape response threshold. If the sulci bordering this region were included in the ablation, the threshold increase was greater and longer-lasting. Ablations of the primary somatosensory cortex increased latencies at most intensities of electric shock, but did not change the response thresholds. Ablations of both primary and second somatosensory cortex increased response thresholds and response latencies. None of these ablations altered the responses, however, if the cat had been overtrained for six to seven months prior to surgery. The results suggest that the second somatosensory region and the sulci bordering the second somatosensory region in the cat cerebral cortex are involved in responses to noxious stimulation.
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  • 24
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    Experimental brain research 21 (1974), S. 45-66 
    ISSN: 1432-1106
    Keywords: Visual cortex ; Efferent cells ; Non-efferent cells ; Synaptic connection ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Neuronal connections in the visual cortex of cat (areas 17 and 18) were studied with intracellular recording and electrical stimulation techniques under Nembutal anaesthesia. Four types of axonal projection were seen; 1. association efferent cells projecting to adjacent cerebral cortex on the ipsilateral side, 2. commissural efferent cells to visual cortex on the contralateral side, 3. corticofugal efferent cells to the ipsilateral lateral geniculate body and superior colliculus, and 4. non-efferent cells whose projection is confined within the visual cortex. Both association and commissural efferent cells were located in layer III, corticofugal efferent cells in layer V and non-efferent cells in layers II–VI. Upon these cells two types of synaptic actions were exerted by the specific visual afferents that originate from the lateral geniculate body; 1. type I, monosynaptic excitation plus disynaptic inhibition and 2. type II, disynaptic excitation plus trisynaptic inhibition. Type I effects were found in layers III–V, and type II in layers II and VI. In the border region between areas 17 and 18 monosynaptic excitation and disynaptic inhibition were produced also by the commissural efferents originating from the contralateral visual cortex. On the basis of these results, a possible neuronal circuitry in the visual cortex is postulated.
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  • 25
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    Experimental brain research 21 (1974), S. 501-513 
    ISSN: 1432-1106
    Keywords: Lateral reticular nucleus ; Spinoreticular tract ; Cerebellum ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The activation of neurones in the mLRN (major portion of lateral reticular nucleus comprising its parvi- and magnocellular parts) by two spinal tracts ascending through the ventral part of the lateral funiculus has been studied by recording from 277 neurones of which 187 could be antidromically activated by stimulation of the cerebellar surface. Forty-eight % of the neurones were activated by the bVFRT and described in a previous paper (Clendenin et al., 1975b) and 12% were activated by a previously unknown tract denoted the ipsilateral forelimb (iF) tract and described in the present paper. 2. The iF-tract is activated by stimulation of nerves in the ipsilateral forelimb only. The response consists of a train of impulses with a high initial frequency and an almost constant latency. Cutaneous afferents and high threshold muscle afferents contribute to the excitation which is mediated by interneurones. 3. The responses to stimulation of peripheral receptors were weak and difficult to classify. 4. The iF-tract activates neurones in the dorsolateral portion of the magnocellular part of the mLRN which projects to the ipsilateral pars intermedia of the anterior lobe and the ipsilateral paramedian lobule. 5. The possible information carried by the iF-tract is discussed.
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  • 26
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    Experimental brain research 21 (1974), S. 433-439 
    ISSN: 1432-1106
    Keywords: Superior colliculus ; Visual cortex ; Layer V-pyramids ; Clare Bishop area ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Stereotaxic injection of horseradish peroxidase into the superior colliculus produced retrograde labelling of layer V pyramides in the Clare Bishop area and the lateral bank of the suprasylvian sulcus, in area 17,18 and 19. Single labelled cells were also found scattered in the splenial, the suprasplenial, the lateral and the suprasylvian gyri. In the cruciate sulcus no labelled cells were observed. Autoradiographically, the lateral bank of the suprasylvian sulcus was also shown to give rise to fibres to the superior colliculus.
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  • 27
    ISSN: 1432-1106
    Keywords: Lateral reticular nucleus ; Bilateral ventral flexor reflex tract (bVFRT) ; Spino-reticulo-cerebellar path ; Cerebellum ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Neurones in the lateral reticular nucleus (LRN) activated by the bilateral ventral flexor reflex tract (bVFRT-LRN neurones) were identified by their responses to stimulation of fibres descending in the contralateral ventral quadrant of the spinal cord which activate the bVFRT neurones monosynaptically. 2. Eighty-eight of the 134 bVFRT-LRN neurones encountered could be antidromically activated at a low stimulus strength from small areas of the cerebellar surface assumed to represent cortical termination points. 3. The bVFRT-LRN neurones occurred throughout the parvi- and magnocellular parts of the LRN and terminated bilaterally in the anterior lobe and sparsely in the ipsilateral paramedian lobule and in the pyramis. 4. The responses evoked in the bVFRT-LRN neurones on stimulation of the contralateral ventral quadrant at C5 and L1 made it possible to estimate the approximate spinal level from which the bVFRT fibres originated. On this basis the bVFRT-LRN neurones were divided into cervical, thoracic, and lumbar groups receiving bVFRT afferents from mainly one spinal level and a convergence group receiving bVFRT afferents from several levels. 5. The different bVFRT-LRN groups received excitation from different combinations of nerves in the four limbs. 6. The cervical, thoracic and lumbar groups of the bVFRT-LRN neurones occurred in successively more ventrolateral portions of the LRN. 7. The cervical, thoracic and lumbar groups of the bVFRT-LRN neurones terminated in successively more rostral areas of the anterior lobe. 8. The organization of the bVFRT-LRN path is compared with that of the ventral and rostral spinocerebellar tracts. The possible function of the bVFRT-LRN path is discussed.
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  • 28
    ISSN: 1432-2013
    Keywords: Splanchnic Afferents ; G.I. and Peritoneal Receptors ; Cortical Projections ; Microelectrode Technique ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The experiments were performed on cats anesthetized with chloralose or Nembutal. The unitary activity of cortical neurons was recorded in the SI and SII areas by means of extracellular glass microelectrodes. The neurons showed frequently a spontaneous discharge (irregular or regular tonic discharge bursts of spikes). The electrical stimulation of contralateral splanchnic nerves produced different effects on a great number of the neurons: 1. early short activation, 2. late activation, 3. early and late complex activation, 4. partial or total inhibition of the spontaneous activity. These effects were obtained in the different cortical layers. Similar results have been observed by mechanical stimulation of the splanchnic receptors located in the gastro-intestinal tract and in the peritoneum. However in this case, the duration and the amplitude of inhibition and activation were generally weaker.
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  • 29
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    Cell & tissue research 148 (1974), S. 577-586 
    ISSN: 1432-0878
    Keywords: Node of Ranvier ; Axonal-glial junctions ; Cat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The paranodal zone of myelinated nerve fibers from the CNS of cats has been studied by means of the lanthanum technique and after freeze-etching. Special interest was given to the axonal-glial zones of contact in this region. Examination of the membranes of the paranodal pockets and the axolemma revealed that the membrane thickenings which occur between the adjacent membranes actually consist of bands. The bands show a specific pattern of diagonal running subunits 10 nm apart. These substructures of the axonal side of the membrane contacts protrude outwards, i.e. towards the glial membrane, where they fit into depressions on the glial membrane surface. This arrangement in form of a “zip-fastener” makes the whole structure very flexible. The subdivision of the bands was identified with both techniques. The different membrane aspects obtained by the freeze-etching technique are discussed in respect to the splitting theory, and a hypothetical model of this membrane specialization is presented.
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  • 30
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    Cell & tissue research 150 (1974), S. 409-423 
    ISSN: 1432-0878
    Keywords: Synaptic organization ; Trochlear nucleus ; Cat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two distinct types of neuron in the cat trochlear nucleus (one large, one small) are described, the α- and γ-motoneurons, respectively. Four types of terminals are observed which establish axo-dendritic synapses. Two of them (Types I and II) perform axo-somatic synapses as well. Terminals en passant (Types I and II) are predominant. The Type I terminal is long and slender with a characteristic distribution of the axoplasmic organelles and the unique feature of a relative narrowing of the synaptic cleft as compared to the width of the neighboring extracellular space. Its vesicle population is pleomorphic and a conspicuous glial barrier surrounds the synaptic zones. The Type II terminal differs slightly from Type I, revealing a wider synaptic cleft and lacking a characteristic distribution of the axoplasmic organelles. The type III terminal is rarely observed performing axo-somatic synapses, but is a common finding in the neuropil. Post-junctional dense bodies are often present in its axodendritic synapses. The Type IV nerve terminal performs axo-dendritic synapses and is characterized by a rich content of large granulated vesicles. Axo-axonal synapses are observed only very rarely. The synaptic organization of the feline trochlear nucleus is compared with the synaptic morphology of the oculomotor nuclei of inframammalian species (Waxman and Pappas, 1971). In addition to certain similarities (e.g., richness of synapses en passant), significant differences are encountered: the present study provides no morphological evidence for electrotonic transmission in the trochlear nucleus of cat.
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  • 31
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    Cell & tissue research 151 (1974), S. 489-498 
    ISSN: 1432-0878
    Keywords: Limbic system ; Cat ; Amygdaloid body ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present study was undertaken to re-examine the central nucleus of the cat in Nissl stained sections, to describe its fine structural characteristics, and to compare the lateral subdivision of this nucleus with the putamen. In Nissl preparations, it was observed that the lateral subdivision of the central nucleus contains a fairly homogeneous population of small cells while the putamen contains both small and large cells. It is the presence of a few large cells at the lateral periphery of the lateral part of the central nucleus that makes the boundary between the two nuclei indistinct. Examination of the fine structure of the medial and lateral subdivisions of the central nucleus revealed the presence of many boutons containing flattened vesicles and fewer with spheroid vesicles. In contrast, most of the boutons in the putamen have spheroid vesicles. It is concluded that the lateral subdivision of the central nucleus may be distinguished from the putamen, except at its most lateral border, by its homogeneous population of small cells and its many boutons containing flattened vesicles.
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  • 32
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    Cell & tissue research 149 (1974), S. 121-135 
    ISSN: 1432-0878
    Keywords: Astrocytic membranes ; Cat ; Orthogonal particle-complexes ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fixed and unfixed astrocytic membranes from the CNS of the cat were studied by means of the freeze-etching technique. A variable number of gap junctions was detected in astrocytic membranes. They are characterized by the well known hexagonal composition of their subunits. Besides this type of highly ordered membrane-bound particles, a second one was found. It is composed of four single particles (diameter 5 nm) which form an orthogonal subunit with a side length of about 10 nm. These membrane-associated orthogonal particle complexes (MOPC) could be observed in different stages of aggregation and expansion. They reveal an accumulation in membranes of the marginal glia layers and in the perivascular astrocytic end-feet. Unfixed, glycerol treated membranes, however, do not show these structures. After glycerol treatment of the unfixed membranes by immersion, the MOPC disintegrate to single particles which form clusters of various extension. The clustering phenomenon is dependent on the length of the time of exposure to glycerol. Shortening of the glycerol treatment by intravasal perfusion of the cryoprotectant agent causes an decrease of the clusters. Fragments and transient forms of the MOPC become visible. By variation of different physico-chemical parameters of the washing solution a similar effect on the MOPC was not achieved. The discussion deals with probable functional aspects of the MOPC. They are considered to act as membrane-bound functional multienzyme complexes which a) might play a role in mediating transmembrane passage of metabolites, or b) are essential for CSF control mechanisms, or c) have a functional relation to the nexus.
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  • 33
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    Cell & tissue research 150 (1974), S. 143-145 
    ISSN: 1432-0878
    Keywords: Arteria centralis retinae ; Cat ; Innervation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Terminal axons emerging from the inner plexiform layer of the cat retina reach the wall of the arteria centralis retinae, as revealed by electron microscopy. Numerous unusually large dense core vesicles (about 1000 Å in diameter), of different electron densities, occur in the varicosities of these axons. These observations may be compatible with the idea of an innervation of the central artery of the retina which is non-autonomic, possibly intrinsic in nature.
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  • 34
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    Cell & tissue research 148 (1974), S. 565-576 
    ISSN: 1432-0878
    Keywords: Central myelin ; Junctions ; Cat ; Freeze-etching ; Lanthanum impregnation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In male and female cats central myelin was studied using the freeze-etching technique, and after impregnation with lanthanum as a tracer. The freeze-fractured and etched faces of the myelin reveal membrane formations which in many respects resemble tight junctions. The formations consist of parallel rows of particles on one half of the membranes matched by corresponding furrows on the other membrane half. These structures occur across the width of the myelin sheath and are most common in that part of the myelin underlying the external cytoplasmic loop. They are interpreted as correlates of the radial component of the i-line which occurs in cross-sections of central myelin after potassium permanganate staining. In the paranodal zone the lanthanum-impregnated myelin displays punctate fusions between the outer leaflets of the oligodendrocytic membranes. These findings strongly suggest that tight junctions are a common feature in the central myelin. The arrangement of the membrane fusion in relation to the elements of the lamellar complex and their functional significance is discussed.
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  • 35
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    Cell & tissue research 151 (1974), S. 499-508 
    ISSN: 1432-0878
    Keywords: Limbic system ; Cat ; Amygdaloid body ; Hypothalamus ; Stereotaxic lesions ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to determine the origin of the hypothalamo-amygdaloid connections in the cat, small lesions were placed at various rostro-caudal levels of the hypothalamus. The animals were sacrificed after a period of 4, 8 or 11 days and the brains stained with the Nauta (1957), Fink and Heimer (1967) or Wiitanen (1969) method for the demonstration of degenerating axons and their terminals. It was observed that the anterior hypothalamic nucleus sends a small projection to the medial subdivision of the central nucleus and to the basal and lateral nuclei of the amygdala. The lateral preoptic area sends a larger projection to the anterior amygdaloid area, both subdivisions of the central and basal nuclei, and to the lateral and medial nuclei. No degeneration was observed in the amygdala following lesions in the medial preoptic area, the ventromedial nucleus or the lateral hypothalamic area caudal to the anterior hypothalamic area. In a series of animals with lateral preoptic lesions, the site of termination of degenerating boutons on neurons of the amygdaloid nuclei was determined and the course of the degenerative process followed over a period ranging from 2 to 15 days. Many of the boutons, especially in the earlier stages of degeneration, appeared to be of the B3 type, containing flattened vesicles and forming symmetrical synaptic contacts with dendrites or somata. With longer post-operative survival times, however, they became increasingly electron dense and shrunken, so that the bouton type could not be determined.
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  • 36
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    Langenbeck's archives of surgery 335 (1974), S. 263-272 
    ISSN: 1435-2451
    Keywords: Coating ; Blood-Compatibility ; Gels ; Chemistry ; Hydrophilic Monomers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurden die wichtigsten Arbeiten über hydrophile Gele ausgewählt und im Hinblick auf ihre Eignung als aussichtsreiche Blut-Kontaktmaterialien betrachtet. Die Reaktionsbedingungen werden beschrieben, und die gegenseitige Wechselwirkung von Blut und Kunststoff wird dargestellt.
    Notes: Summary By the selection of the relevant sources it is attempted to present the contemporary research situation of the hydrophilic polymeric gels as promising materials for the contact with the blood in the organism. The reaction conditions of the current syntheses are described as well as the mutual interactions of the blood and the contact surface.
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  • 37
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    New York : Wiley-Blackwell
    Biopolymers 13 (1974), S. 1-27 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We describe a method for determining chemical kinetic constants and diffusion coefficients by measuring the rates of decay of spontaneous concentration fluctuations. The equilibrium of the system is not disturbed during the measurement. We measure the number of molecules of a specified type in a defined open volume as a function of time and compute the time course of the deviations from the thermodynamic mean concentration. The method is based on the principle that the rates of decay of spontaneous microscopic fluctuations are determined by the same phenomenological rate coefficients as those of macroscopic departures from equilibrium which result from external perturbations. Hence, an analysis of fluctuations yields the same chemical rate constants and diffusion coefficients as are measured by conventional procedures. In practice the number of the specified molecules is measured by a property such as absorbance or fluorescence which is specific and sensitive to chemical change. The sample volume is defined by a light beam which traverses the cell. As the molecules appear in or disappear from the light beam, either due to diffusion or chemical reaction, their concentration fluctuations give rise to corresponding fluctuations of the intensity of absorbed or emitted light. This paper presents the theory needed to derive chemical rate constants and diffusion coefficients from these fluctuations in light intensity. The theory is applied to three examples of general interest: pure diffusion in the absence of chemical reaction; the binding of a small rapidly diffusing ligand to a larger slowly diffusing macromolecule; and a unimolecular isomerization. The method should be especially useful in studying highly cooperative systems, relatively noncooperative systems with intermediate states closely spaced in free energy, small systems, and systems not readily subject to perturbations of state.
    Additional Material: 3 Ill.
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  • 38
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    Biopolymers 13 (1974), S. 97-114 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The molecular conformations and dynamics of poly(L-prolyl), poly(hydroxyl-L-prolyl), poly(L-prolyl-glycyl), poly(hydroxyl-L-prolyl), and poly(glycyl-glycyl-L-prolyl-glycyl), in aqueous solution, have been studied using 13C pulse Fourier transform nmr spectroscopy. From a measurement of the intensities of major and minor resonances in the spectra of the copolypeptides, it was determined that 15-20% of the glycyl-prolyl and glycyl-hydroxyprolyl peptide bonds are cis. Effective rotational correlation times (τeff), obtained from measurements of spin-lattice relaxation times (T1) of individual backbone and side-chain carbons, demonstrated that backbone reorientation is approximately isotropic for the five polypeptides and is characterized by correlation times of ca. 0.3-0.6 nanoseconds as a result of rapid segmental motion. In a given polypeptide glycyl and pyrrolidine residues were found to have the same backbone correlation times, but backbone carbon τeff values did decrease as the glycyl content of the peptides increased. A semi-quantitative analysis of Cβ, Cγ, and Cδ correlation times suggests that rapid ring motion in both prolyl and hydroxyprolyl involves primarily Cγ and Cβ, with the prolyl ring being more mobile than the hydroxyprolyl ring.
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  • 39
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    Biopolymers 13 (1974), S. 237-238 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No. Abstract.
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  • 40
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    Biopolymers 13 (1974), S. 217-226 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A model for the flexibility of DNA is proposed that is based on discrete variations in the direction of propagation in going from one subunit to the next. Expansion of the local free energy in terms of the local bending gives a Gaussian distribution function. The assumption of the independence of local bends on neighbors lead to very simple formulae for the persistence length and the characteristic ratio. Emphasis, however, is placed on the application of the formulae for molecules of finite size where the persistence length and C∞ are not defined. The formulae are worked out for two models, which should serve as limits for the real physical situation.
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  • 41
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    Biopolymers 13 (1974), S. 735-743 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: If dispersion effects are not taken into account, the dimensions of particles in suspension estimated from spectrophotometric data (slope of the log OD versus log λ plot) are too low. By measuring dispersion curves for the medium and for the suspension, a consistent estimate of the size of latex particles of different diameter is obtained from the OD data.
    Additional Material: 3 Ill.
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  • 42
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    Biopolymers 13 (1974), S. 765-768 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The helix-coil transition of poly-N5-(3-hydroxypropyl)-L-glutamine in methanol-water solutions has been observed using 13C nuclear magnetic resonance. Two signals appear in the α-CH region in the course of the transition; this is in contrast with previous proton magnetic resonance experiments carried out on this polymer in the same solvent system.
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  • 43
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    Biopolymers 13 (1974), S. 779-790 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: X-ray diffraction and stereochemical analyses were used to study the hydrated structure of the helical amylose polymorph having a fiber repeat spacing of 8 Å. Intensity calculations using computer models confirmed six as the number of residues per turn and supported the space group P212121. Both diffraction intensity and stereochemical methods indicate the suitability of residue G3 from the potassium acetate complex of cyclohexaamylose as opposed to residues with longer O(4)-O(1) vectors. Comparison of the present results with those obtained for V amylose dehydrate indicated no major conformational differences between the two helix skeletons. A net helical rotation of approximately 30° accompanied the hydrate-dehydrate transition and the rotational position in the hydrate allowed packing that was less close. Hydration water molecules were not located; noncarbohydrate peaks on the electron density maps were primarily due to Fourier series termination errors.
    Additional Material: 4 Ill.
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  • 44
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    Biopolymers 13 (1974), S. 843-852 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We have measured the circular dichroism spectra and have obtained melting curves for samples of purified satellite DNA's from three species of crab, Cancer borealis, Gecarcinus lateralis, and Callinectes sapidus. Although all three satellite DNA's have the same density in neutral CsCl, their circular dichroism spectra and melting and reassociation characteristics are different, as are other properties of these DNA's. The circular dichroism spectra are analyzed to give estimates of nearest-neighbor frequencies. We estimate that about 90% of the Cancer borealis satellite DNA and over 80% of the Gecarcinus lateralis satellite DNA consist of d(AT)n:d(AT)n sequences, while the serially repeating unit of the Callinectes sapidus satellite DNA may not contain any extended d(AT)n:d(AT)n sequences. The results from the circular dichroism spectra for the Cancer borealis, Gecarcinus lateralis, and the Callinectes sapidus satellite DNA's are in agreement with base composition analyses, which give 2.4, 8, and 25% G+C, respectively, for these DNA's.
    Additional Material: 3 Ill.
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  • 45
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    Biopolymers 13 (1974), S. 879-890 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Samples of epichlorohydrin crosslinked potato starch were prepared by using a high ratio of starch to water and alkali concentration below the gelatinization level. This yielded crosslinked samples that were partially crystalline, and where the number of crosslinks could be varied between 1 and 20 crosslinks per 100 anhydroglucose units. The degree of swelling varied regularly with degree of crosslinking, and the molecular weight between crosslinks Mc as derived from swelling data in a good swelling agent compared favorably with Mc derived from chemical analysis.From the heat of gelatinization of the crosslinked starches, as observed in a differential scanning calorimeter, for gelatinization in glycerol, water, and dimethylsulfoxide, a model for the gel state of the crosslinked starch is proposed. It is concluded that the entropy of melting is the determining factor in establishing the temperature of gelatinization.
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  • 46
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    Biopolymers 13 (1974), S. 909-917 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Circular dichroism studies of the interaction of β-cyclodextrin with a series of eight dinucleoside phosphates, and with 3′-AMP are reported. From results with ApU and ApA, it is shown that β-cyclodextrin binding is sensitive to dinucleoside stacking in approximately the same way as optical measures of stacking. Some qualitative uses of β-cyclodextrin binding are suggested, based on the fact that the change in the CD spectrum caused by cyclodextrin binding is unique to each of the dinucleosides studied. Hoffman and Bock have previously suggested the use of β-cyclodextrin as a probe of nucleic acid structure. Their work indicated that only binding to adenosine and inosine would have to be considered. The present paper shows that binding to other bases cannot be neglected, and will impose serious restrictions on the use of β-cyclodextrin as a structural probe.
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  • 47
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    Biopolymers 13 (1974), S. 987-993 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Poly(1,N6-etheno-2-aza-adenylic acid) [poly(2-aza-εA)] was synthesized from 1,N6-etheno-2-aza-adenosine 5′-diphosphate (2-aza-εADP) and Escherichia coli polynucleotide phosphorylase. The values Km = 1.02 mM, V = 1.06 μmol hr-1 enzyme unit-1 were found for the polymerization reaction. In contrast to polyadenylic acid, this novel fluorescent polymer has a random structure in solution. The application of the 2-aza-εADP for localization of polynucleotide phosphorylase was also described.
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  • 48
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    Biopolymers 13 (1974), S. 1011-1022 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A series of L-methionine oligomers [BOC-(Met)n-OMe] (n = 2-7) and the corresponding diastereomeric di- and tripeptides were synthesized using the mixed anhydride method. Oligomers prepared in this manner were optically pure and were obtained in reasonable yield. Preliminary optical examination of the peptides suggests that secondary structures may begin forming in the pentamer or hexamer in trifluoroethanol. BOC-(Met)4-OMe and BOC-(Met)5-OMe were also synthesized using an insoluble resin containing BOC-L-methionine as the nitrophenol active ester.
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  • 49
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conformational studies of a series of oligopeptides (from the tripeptide to the octadecapeptide) with the repeating sequence L-norvalyl-glycyl-L-proline and a polytripeptide with this sequence are reported. By means of chiroptical techniques, unordered conformations are found for all oligopeptides in water, trifluoroethanol, and ethylene glycol and for the water-insoluble polymer in trifluoroethanol. In ethylene glycol the polymer assumes a collagen-like structure. Infrared studies indicate that all the oligomers, in contrast to the polymer, are unordered in the solid state.
    Additional Material: 8 Ill.
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  • 50
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    Biopolymers 13 (1974), S. 1109-1116 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Uncharged poly(Nε-methyl-L-lysine) (PMLL) and its isomer, poly(Nδ-ethyl-L-ornithine) (PELO), in alkaline solution (pH ca. 12) undergo a helix-to-β transition upon mild heating at 50°C or higher in a manner similar to that of poly(L-lysine) (PLL). The rate of conversion follows the order: PMLL 〈 PELO 〈 PLL. The helix can be regenerated upon cooling near zero degrees, for instance, after more than 12 hr at 2°C. At concentrations less than 0.02% the β form is intramolecular, but at higher concentrations both intra- and intermolecular β forms are generated. Poly(Nδ-methyl-L-ornithine) (PMLO), an isomer of PLL, behaves like poly(L-ornithine); uncharged PMLO in alkaline solution is partially helical and becomes disordered at elevated temperatures.
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  • 51
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    Biopolymers 13 (1974), S. 1161-1171 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In order to study the effect of side-chain length on the conformation of polypeptides, conformational changes of various ionic polypeptides with various lengths of side chain, poly-Nε-glutaryl-L-lysine (PGL), poly-Nδ-glutaryl-L-ornithine (PGO), poly-Nε-succinyl-L-lysine (PSL), and poly-Nδ-succinyl-L-ornithine (PGO), were investigated by ORD, potentiometric titration, and dilatometric measurements in aqueous solution. The results of optical rotation and potentiometric titration measurements indicate strongly that the α-helix stability increases in the sequence PSO 〈 PSL ∼ PGO 〈 PGL, which corresponds to increased side-chain length. The volume change associated with the helix-coil transition also increased in the above sequence. This series of polymers seems to be more hydrophobic compared with poly-L-glutamic acid or poly-L-lysine, as suggested from the values of enthalpy and entropy changes for coil-helix transitions.
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  • 52
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    Biopolymers 13 (1974), S. 1195-1203 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformational changes of poly-Nε-glutaryl-L-lysine (PGL) and poly-Nε-succinyl-L-lysine (PSL) in various salt solutions were studied by use of ORD and potentiometric titration measurements. The addition of alkali metal salts to the fully ionized PGL or PSL solution caused helix formation. The helical content of the polymers increases in the following sequences: at salt concentration 0-2 M, CsCl 〈 KCl 〈 LiCl 〈 NaCl; and at 2-3 M, LiCl 〈 CsCl 〈 KCl ∼ NaCl. The preferential binding of the solvent components with various alkali metal salts of PGL or PSL was measured in LiCl, NaCl, and KCl solutions by means of equilibrium dialysis and differential refractometry. It was found that with increasing salt concentration, the polymers were preferentially hydrated in NaCl and KCl soultions; however the salt was preferentially bound to the polymers in LiCl solution. Such preferential binding was suggested to be closely related to conformational change. The addition of CaCl2 to polymer solutions led to the stabilization of the helical structure of PGL or PSL.
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  • 53
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    Biopolymers 13 (1974), S. 1261-1267 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Refractive indices of bovine serum albumin solutions in saline and in 2-chloro ethanol are measured for the spectral region 1770-10000 Å by a reflectance method. A procedure for the determination of a dispersion formula for binary mixtures is developed and applied. The electronic polarizability of bovine serum albumin is determined and is found to be almost independent of the solvent and concentration. Hence it follows that the electronic polarizability of bovine serum albumin is almost independent of the molecular environment.
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  • 54
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    Biopolymers 13 (1974) 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 55
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The dielectric absorption of poly-DL-phenylalanine and poly-γ-benzyl-L-aspartate (PLAB) was measured in very dilute solutions to determine the type of molecular association and to locate the helix-coil transition. Both polypeptides were present as associated helices in chloroform. The mode of aggregation, which was determined by measuring the dipole moment and the critical frequency, did not depend on the polarity of the side chain but rather on that of the solvent.In both polymers, the dissociation of the aggregates in chloroform was observed on addition of small amounts of dichloroacetic acid; further addition of the acid lead to the helix-coil transition.No second region of dielectric absorption that might be related to the kinetics of the transition was found during the helix-coil transition of PBLA.
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  • 56
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Molecular conformation of some polysaccharides in aqueous solution in evidenced by changes in the optical rotation and in the elution pattern of gel filtration. The changes in the specific rotation against that in water are expressed as a molar conformational value [K]D21: -495° for colominic acid in 1.0 N NaOH solution, and -180° for hyaluronate (HA), +85° for corneal keratin sulfate, and +234° for amylose in 8 M urea solution. The gel filtration of amylose and HA dissolved in 8 M urea solution shows elution patterns distinctly different from those dissolved in water. The phenomena are attributable to a molecular conformational transition of polysaccharide molecules in aqueous solution.
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  • 57
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A previously described procedure for simultaneous optimization of bond lengths and angles was used to test different models for mannan I. Potential hydrogen bonds and the glycosidic angle were included in the optimization. A conformational model with bifurcated intramolecular hydrogen bonds of the type observed in the methyl cellobioside methanol complex showed the best agreement with available exprerimental data. The coordinates of this model were provided by computer calculations. The available X-ray data, however, were not sufficient for selecting this model; rather, ir data were necessary to furnish the needed information. The different conformational models tested all showed an almost constant virtual bond length O(1)-O(4) of the β-pyranose residue. This was in contrast to the previously obtained results for the α-pyranose residues.
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  • 58
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    Biopolymers 13 (1974), S. 1187-1193 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The longitudinal proton magnetic relaxation times T1 were measured for ferri (met)-and carbonmonoxy-bovine haemoglobin and equine myoglobin in 0.1 M KH2PO4 aqueous solutions near pH 6 at 5°C and 35°C from 1.5- to 60-MHz Larmor frequencies. It is concluded that the correlation time τC for the dipole-dipole interaction of electron and nuclear spins is in fact the electron (ferric) spin relaxation time τS being close to 1.5 × 10-10 sec for both metHb and metMb at 5°C. At 35°C the paramagnetic relaxation rates are not determined solely by the relaxation of protons exchanging from the haem pocket with bulk solvent. Hence, τC at 35°C cannot be calculated from the dispersion data obtained at this temperature. The relevance of this for the determination of interspin distances r is discussed.
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  • 59
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    Biopolymers 13 (1974), S. 1213-1222 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The orientation of naphthalene rings in the side chain of poly-γ-1-naphthylmethyl-L-glutamate (PNLG) in mixed solvents of dichoroethane (DCE) and hexafluoroisopropanol (HFIP) has been studied together with its conformation by infrared, circular dichroism, and fluorescence spectra. The CD pattern of PNLG varies with the solvent composition while it maintains the α-helical conformation. The fluorescence spectra of PNLG in solution show excimer emission of the naphthalene chromophores. The ratio of intensity of the excimer emission to that of the normal fluorescence decreases as the HFIP component in the solvent increases. It is suggested that the naphthalene rings in the side chain of α-helical PNLG are more rigidly orientated in the solvents of higher HFIP ratio.
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  • 60
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    Biopolymers 13 (1974), S. 1269-1290 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The near ultraviolet chiroptical properties of L-cystine conformational isomers are examined on a static, “one-electron” model in which the disulfide moiety is the chromophoric group and the atoms of the L-alanyl fragments are treated as perturbers. The zeroth order chromophoric wave functions are calculated on a semiempirical molecular orbital model in which excited states are constructed in the virtual orbital-configuration interaction approximation. The perturbing environment is represented by point charges located at the atomic centers of the L-alanyl fragments. Chromophore-perturber interactions are expressed as charge-multipole moments with only the charge-dipole and charged-quadrupole terms being retained in the calculations. Vicinal contributions to the rotatory strengths of the five lowest energy disulfide transitions are computed for 30 conformational isomers of the L-cystine dizwitterion. The results provide support for the view that vicinal or peripheral effects can account entirely for the observed near ultraviolet (λ 〉 230 nm) chiroptical properties of L-cystine and its derivatives and that these properties are diagnostic of conformational features external to the disulfide moiety.
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  • 61
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The circular dichroic spectrum of poly(Tyr,TyrBr) in trimethylphosphate from 180 to 320 nm is found to be a linear function of composition from 25 to 68% TyrBr, and the spectra of the Tyr and TyrBr components are resolved. For Tyr the resolved spectrum is in agreement with available CD spectra of poly(Tyr). These results are interpreted to mean that the side-chain conformations of Tyr and TyrBr are not affected by the other component, and that for Tyr the conformation is the same as in poly(Tyr). It is also concluded that poly(Tyr,TyrBr) is helical with the same helix sense as poly(Tyr). The restrictions on the side-chain conformations are discussed, and it is suggested that the Tyr and TyrBr side chains are all in the same χ1 region.
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  • 62
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    Biopolymers 13 (1974), S. 1377-1389 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The circular dichroism of double-helical nucleic acids was calculated as a function of geometry using the theory of Tinoco and Johnson. This theory does not include contributions of near ultraviolet transitions that are not π → π* in nature. The calculated circular dichroism shows a strong dependence on the distance of base pairs from the helix axis and the tilt of the base pair. Smaller dependences are predicted for the propeller-like twist of a base pair and for variation of the angular increment per base pair. Moderately good agreement between calculated and many experimentally observed spectra could be generated.
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  • 63
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    Biopolymers 13 (1974), S. 1427-1434 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The maximum at 220 nm in the circular dichroism spectrum of native collagen solution changed to a negative value after heat denaturation or collagenase hydrolysis. The enzyme induced rate of CD change at 220 nm was shown to be first order in collagen concentration. The specific rate constant k is actually a combined rate constant kfast and kslow in which the ratio kf/ks is 4.1. The initial rates were linear with respect to enzyme concentration, and the Km was found to be 5.5 × 10-7 M. The rate of ultraviolet hyperchromicity at 220 nm on collagen hydrolysis was determined. The kfast was the same as that obtained by CD. The kf/ks ratio was 4.6. Both methods may be readily used to assay for collagenase activity.
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  • 64
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    Biopolymers 13 (1974), S. 1461-1482 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The crystal and molecular structure of the complex of amylose with dimethyl sulfoxide has been studied by a combination of stereochemical analysis, potential energy, and X-ray diffraction methods. The complex crystallizes in a pseudotetragonal unit cell with a = b = 19.17 Å and c (fiber axis) = 24.39 Å, with two antiparallel chains per unit cell and space group P212121. The amylose chain is a left-handed 61(1.355) helix with three turns per crystallographic repeat. The O(6) rotational position is approximately gt. Dimethyl sulfoxide is located inside the helix with one DMSO molecule for every three glucose residues. An additional four DMSO molecules and eight water molecules each are located in the large interstices between chains, and it is the interaction of these molecules with the helix that results in the pseudotetragonal chain packing. The interstitial DMSO is the source of the previously reported additional layer lines, which are not consistent with the 8.13-Å amylose repeat distance. The final R factor for the layers with amylose contribution to the structure factors was 0.29, while the overall R factor was 0.35. The stereochemical packing analysis provided suitable phasing models for the subsequent X-ray refinement.
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  • 65
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    Biopolymers 13 (1974), S. 1549-1556 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dielectric dispersion of DNA was studied in the frequency range 100 Hz-100 kHz at four different temperatures (6-30°C). The dielectric increment ε0-ε∞ increased with the rise of temperature. The relaxation time, on the other hand, decreased. Both the increase in dielectric increment and the decrease in relaxation time could not be explained on the basis of the counterion polarization theory. Dipole moment was estimated from Kirkwood theory. It was found to decrease systematically with temperature. Even at 0°C there was a dipole moment of 104D.
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  • 66
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    Biopolymers 13 (1974), S. 1573-1605 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The dependence of Förster long-range resonance energy transfer efficiency on the orientational freedom of donor D and acceptor A molecules attached to a macromolecular substrate is examined. The usefulness of polarized emission measurements in determining the mutual orientation as well as the degree of orientational freedom of D and A and thereby deriving maximum and minimum values for the D-A separation from the transfer efficieny is demonstrated.
    Additional Material: 20 Ill.
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  • 67
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    Biopolymers 13 (1974), S. 1653-1659 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Five human hemoglobins whose genetic relationship to one another involves one set of alleles, hemoglobins CC, SC, SS, AS, and AA, were studied in the deoxygenated form within a McBain gravimetric sorption system at 28°C. Water was used as the adsorbate at relative vapor pressures ranging from 0.11 to 0.39. The results of this study are reported and compared with a similar study on the ferric form of these same five hemoglobins. For both forms of hemoglobin studied, a slight increase was measured in the number of polar sites accessible to H2O vapor for those genotypes in which the substituent in the sixth position from the N terminus of the two β chains had a positively charged side chain, i.e., hemoglobin C. Those samples containing hemoglobin S (less soluble in the deoxy form) show an increased BET monolayer coverage in the deoxy as compared to the ferric form. For each genotype, the deoxygenated form had more polar sites accessible to H2O vapor than its ferric form. This finding is in agreement with an increase in the size of the central cavity as shown by crystallographic analysis.
    Additional Material: 1 Ill.
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  • 68
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Thermal denaturation of direct-mixed and reconstituted polylysine-DNA complexes in 2.5 × 10-4 M EDTA, pH 8.0 and various concentrations of NaCl has been studied. For both complexes, increasing ionic strength of the solution raises Tm, the melting temperature of free base pairs. The linear dependence of Tm on log Na+ indicates that the concept of electrostatic shielding on phosphate lattice of an infinitely long pure DNA by Na+ can be applied to short free DNA segments in a nucleoprotein. For a direct-mixed polylysine-DNA complex, the melting temperature of bound base pairs Tm′ remains constant at various ionic strengths. On the other hand, the Tm′ in a reconstituted polylysine-DNA complex is shifted to lower temperature at higher ionic strength. This phenomenon occurs for reconstituted complex with long polylysine of one thousand residues or short polylysine of one hundred residues. It is shown that such a decrease of Tm′ is not due to a reduction of coupling melting between free and bound regions in a complex when the ionic strength is raised. It is also not due to intermolecular or intramolecular change from a reconstituted to a direct-mixed complex. It is suggested that this phenomenon is due to structural change on polylysine-bound regions by ionic strength. It is suggested further that Na+ may replace water molecules and bind polylysine-bound regions in a reconstituted complex. Such a dehydration effect destabilizes these regions and lowers Tm′. This explanation is supported by circular dichroism (CD) results.
    Additional Material: 11 Ill.
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  • 69
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Theoretical values are reported for the specific conductivity, charge carrier mobilities, and delocalized free paths in β-polyglycine using the parallel-chain pleated sheet conformation and the all-valence electron energy band structure obtained previously. The mobilities are found to be much larger along the polypeptide backbones than in the direction of the hydrogen bonds. This indicates a mechanism of conduction in which the former path is favored for electronic delocalization. The preexponential factor of the specific conductivity obtained theoretically agrees well with experimental results.
    Additional Material: 1 Tab.
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  • 70
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    Biopolymers 13 (1974), S. 1747-1750 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The circular dichroism of a series of L-methionine oligopeptides [BOC-(Met)n-OMe] was examined in trifluoroethanol and hexafluoroacetone sesquihydrate. The results indicate that the trimer through the hexamer exists predominantly in disordered conformations in these solvents. An abrupt change in the CD pattern at the heptamer in trifluoroethanol suggests that L-methionine oligopeptides begin forming helices at this chain length.
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  • 71
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    Biopolymers 13 (1974), S. 1767-1776 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The spin-lattice relaxation time (T1) of water adsorbed on collagen fibers was determined at six frequencies and temperatures varying from 25° to -80°C. Care was taken to eliminate the contributions to the signal of protons other than those in the adsorbed water. Quantitative calculations were made on T1 and the results were compared with the experimental data. It is suggested that a maximum of about 0.50-0.55 g water per g collagen forms a hydration layer, which cannot be frozen down to -90°C and exhibits a distribution of motional correlation times. For collagen samples containing a larger quantity of adsorbed water, the additional water molecules behave like ordinary isotropic water, having a single correlation time and a freezing temperature of about -10°C.
    Additional Material: 5 Ill.
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  • 72
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    Biopolymers 13 (1974), S. 1821-1845 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Laser Raman spectra of the trinucleoside diphoshate ApApA and dinucleoside phosphates ApU, UpA, GpC, CpG, and GpU are reported and discussed. Assignments of conformationally sensitive frequencies are-facilitated by comparison with spectra reported here of poly(rA), poly(rC), and poly(rU) in deuterium oxide solutions. The significant spectral differences between ApU and UpA, and between GpC and CpG, reveal that the sequence isomers have nonidentical conformations in aqueous solution. In UpA at low temperature the bases are stacked and the backbone conformation is similar to that found in ordered polynucleotide structures and RNA. In ApU no base stacking can be detected and the backbone conformation differs from that found in UpA, both in the orientation of phosphodiester linkages and in the internal conformation of ribose. At the conditions employed neither ApU nor UpA exhibits base pairing in aqueous solutions. In both GpC and CpG the bases are stacked and the phosphodiester conformations are similar to those encountered for UpA and RNA. However, major differences between spectra of GpC and CpG indicate that the geometries of stacking and ribosyl conformations are different. In GpC the Raman data favor the formation of hydrogen bonded dimers containing GC pairs. Protonation of C in GpC is sufficient to eliminate the ordered conformation detected by Raman spectroscopy. Despite the ordered backbone conformation evident in GpU, this dinucleoside apparently contains neither stacked nor hydrogen bonded bases at the conditions employed here. The Raman data also confirm the stacking interactions in ApApA, poly(rA), and poly(rC) but suggest that the backbone conformation in poly(rC) differs qualitatively from that found in most ordered polynucleotide structures and is thermally more stable. The present results demonstrate the sensitivity of the Raman technique to sequence-related structural differences in oligonucleotides and provide additional spectra-structure correlations for future conformational studies of RNA by laser Raman spectroscopy.
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  • 73
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    Biopolymers 13 (1974) 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 74
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 300-MHz proton nmr spectra (between 11 and 14 ppm) of a series of double-stranded deoxy oligonucleotides of known sequence have been recorded in H2O solution. These resonances have been assigned to the G—N1H and T—N3H protons of specific base pairs from an evaluation of the temperature dependence of the ring NH linewidths and from the selective ring NH chemical shift changes on actinomycin-D binding. The deoxy oligonucleotides exist predominantly in the DNA-B conformation as evaluated from antibiotic binding studies. Ring-current calculations have been utilized to evaluate the up-field shifts of the G—N1H and T—N3H protons in Watson-Crick base pairs due to the ring currents from the pyrimidine and purine rings of nearest neighbor base pairs in regular DNA-B- and RNA-A-type helices. The perturbations on these up-field ring-current contributions that arise from twisting and tilting a base pair adjacent to the ring NH under study have been evaluated and found to change the calculated chemical shift by ±0.6 ppm for twist and tilt distortions of 〈30°C in a single adjacent base pair. A knowledge of the experimentally assigned ring NH chemical shifts of specific base pairs in known sequences of double-stranded deoxy oligonucleotides coupled with the ring-current tables for the DNA-B helical structure permit the assignment of 13.6 ± 0.1 ppm and 14.6 ± 0.2 ppm for the G—N1H proton of an isolated GC base pair and the T—N3H proton of an isolated AT base pair, respectively.
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  • 75
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    Chemie in unserer Zeit 8 (1974), S. A4 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 76
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    Chemie in unserer Zeit 8 (1974), S. 65-71 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 8 Ill.
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  • 77
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    Chemie in unserer Zeit 8 (1974), S. A40 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 78
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    Chemie in unserer Zeit 8 (1974), S. 112-120 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 15 Ill.
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  • 79
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 80
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    Chemie in unserer Zeit 8 (1974) 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 81
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    Chemie in unserer Zeit 8 (1974), S. 159-160 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 82
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    Chemie in unserer Zeit 8 (1974), S. 192-193 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 83
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    Chemie in unserer Zeit 8 (1974), S. A78 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 84
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    Chemie in unserer Zeit 8 (1974), S. 121-123 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 85
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 86
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    Chemie in unserer Zeit 8 (1974), S. 129-134 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 7 Ill.
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  • 87
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    Chemie in unserer Zeit 8 (1974), S. A66 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 88
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    Chemie in unserer Zeit 8 (1974), S. 177-183 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 12 Ill.
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  • 89
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    Chemie in unserer Zeit 8 (1974) 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 90
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    Chemie in unserer Zeit 8 (1974), S. 31-32 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 91
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 92
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    Biopolymers 13 (1974), S. 29-61 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper describes the first experimental application of fluorescence correlation spectroscopy, a new method for determining chemical kinetic constants and diffusion coefficients. These quantities are measured by observing the time behaviour of the tiny concentration fluctuations which occur spontaneously in the reaction system even when it is in equilibrium. The equilibrium of the system is not disturbed during the experiment. The diffusion coefficients and chemical rate constants which determine the average time behaviour of these spontaneous fluctuations are the same as those sought by more conventional methods including temperature-jump or other perturbation techniques. The experiment consists essentially in measuring the variation with time of the number of molecules of specified reactants in a defined open volume of solution. The concentration of a reactant is measured by its fluorescence; the sample volume is defined by a focused laser beam which excites the fluorescence. The fluorescent emission fluctuates in proportion with the changes in the number of fluorescent molecules as they diffuse into and out of the sample volume and as they are created or eliminated by the chemical reactions. The number of these reactant molecules must be small to permit detection of the concentration fluctuations. Hence the sample volume is small (10-8 ml) and the concentration of the solutes is low (∼ 10-9 M). We have applied this technique to the study of two prototype systems: the simple example of pure diffusion of a single fluorescent species, rhodamine 6G, and the more interesting but more challenging example of the reaction of macromolecular DNA with the drug ethidium bromide to form a fluorescent complex. The increase of the fluorescence of the ethidium bromide upon formation of the complex permits the observation of the decay of concentration fluctuations via the chemical reaction and consequently the determination of chemical rate constants.
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  • 93
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    Biopolymers 13 (1974), S. 129-138 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The D and L copolymerizations of N-carboxy γ-benzyl glutamate anhydride (NCA) were carried out in a homogeneous solution with various D/L ratios, initiated by either n-butylamine or sodium methoxide, and were followed directly by circular dichroism (CD) to observe the behavior of the secondary structure of growing polymer molecules. In the n-butylamine system, the difference of the helical content between the righthanded and the lefthanded (Δα-helix) gradually increased as the polymerization proceeded, while in the sodium methoxide system, the Δα-helix had a tendency to decrease during the later stages of the polymerization. These results suggest a difference of the power of stereo-selection of monomer antipodes by the growing chain end between these systems, the stereoselectivity by the growing chain end in the sodium methoxide system being higher than that in the n-butylamine system.
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  • 94
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    Biopolymers 13 (1974), S. 169-183 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The circular dichroism of Ac-Ala-NHMe, cyclo(-Ala-Ala-), Ac-Ala-OMe, Ac-Ala-Ala-OMe, and Ac-Ala-Ala-Ala-OMe has been measured in water and in aqueous salt solutions as a function of temperature. Only cyclo(-Ala-Ala-) exhibits circular dichroism which is independent of temperature. Each of the linear derivatives of L-alanine exhibits a positive circular dichroism in the range 208-218 nm at 15°C in water. Heating reduces the intensity of the positive circular dichroism, and only Ac-Ala-OMe retains positive circular dichroism at 75°C in water. Isothermal addition of salts produces changes in the circular dichroism of linear derivatives of L-alanine which resemble those seen on heating. The relative effectiveness of the salts tested, at a concentration of 4M, is LiCl ≤ KCl = NaCl 〈 MgCl2 ≤ CaCl2 ≤ NaClO4. The circular dichroism of cyclo(-Ala-Ala-) is also affected by the salts. Extrapolation of the results obtained with Ac-Ala-OMe, Ac-Ala-Ala-OMe, and Ac-Ala-Ala-Ala-OMe to a long polypeptide with a -CH2R side chain in the L-configuration leads to the conclusion that this polypeptide should exhibit a temperature-dependent salt-sensitive positive circular dichroism between 208 and 218 nm when it exists as a statstical coil.
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  • 95
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    Biopolymers 13 (1974), S. 227-235 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The heats of the conformational conversion ΔHc of polyuridylic acid (polyU) in the presence of various cations were determined using a differential scanning microcalorimeter and an isothermal mixing calorimeter. The first method yields a value of ΔHc = 5.2 kcal/mol of base pairs, the second one a value of ΔHc = 6.4 kcal/mol of base pairs. Knowing ΔHc and the maximal slope of the degree of conversion, which was determined from the temperature dependence of the absorbance of polyU solutions at 260 nm, we were able to evaluate the parameter of cooperativity σ. From the relation σ = eΔFstack/RT the stacking free energy ΔFstack could be calculated. Dividing the apparent enthalpy of conversion by the calorimetrically measured enthalpy yields the cooperative length N0 of one helical segment at the midpoint of conversion. The results show that the type of the cation has no influence on the magnitude of ΔHc, whereas the cooperativity of polyU is influenced by the various cations.
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  • 96
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    Biopolymers 13 (1974), S. 861-862 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 97
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    Biopolymers 13 (1974), S. 903-908 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Clipped autocorrelation functions for the fluctuating intensity of light scattered from dilute solutions of poly-L-lysine in 0.2 M NaBr have been observed over a wide range of time scales down to 1.83 μsec, and over a wide range of pH. Two poly-L-lysine samples of molecular weight 35,000 and 200,000, respectively, were compared to determine the extent of draining of the so-called random-coil configuration. The observed correlation functions were in general very satisfactorily represented by simple translation diffusion of single molecules. At high pH ∼10-10.4, there was evidence of considerable aggregation, but no indication whatsoever of any unusually fast component, as reported recently by Jamieson et al. for poly-L-lysine HBr in the absence of salt.A 30% rise in the diffusion coefficient to a peak at pH 10 parallels the previously reported dip in the intrinsic viscosity, and is interpreted in terms of a more compact structure in that pH region between the coil and helix extremes.
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  • 98
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformation of deoxyribonucleoprotein (DNP) from calf thymus at different stages of deproteinization was studied.The dissociation of the first portion of histone produces no effect on the hydrodynamical and optical behavior of DNP particles. The conformational transition of a macromolecule was observed as soon as the ratio of protein to DNA ≤ 0.9.The effect of ionic strength on the conformation of DNP particles with high protein content was more strongly pronounced than that for DNA. On the contrary, DNP particles depleted of proteins (protein/DNA 〈 0.9) were found to be less sensitive than DNA to the variation of ionic strength. These data imply that the DNP molecules rich in proteins possess a superstructure that is destroyed as the protein/DNA ratio becomes 0.9.The data were analyzed in view of current theories on various model concepts. The most probable model to describe the DNP molecule was chosen by comparing the calculated and experimentally obtained parameters. We believe that DNP is best described as a “compressed coil,” possibly including superhelical regions.
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  • 99
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis of some high-molecular-weight protenoid structures in radiofrequency cold plasma conditions are discussed. The fractionation results of the raw product and the spectrum of free and protenoid-bound amino acids are given. The uv analysis of these compounds is presented.A protein-forming mechanism that might have existed on the primitive earth is proposed.
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  • 100
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Double-stranded DNA can be readily adsorbed on mica or directly on carbon coated grids from the surface of solutions containing ethidium bromide, actinomine, or propidium diiodide. The DNA molecules are unfolded, well separated, and show a length distribution similar to molecules prepared by protein monolayer techniques.Since the intercalating dyes tested do not lead to an increased apparent diameter of the nucleic acid the method is useful for the study of nucleic acid-protein complexes. As a model, the binding of E. coli RNA polymerase to phage T7 and T3 DNA was examined under different conditions. The enzyme can easily be identified and its position along the DNA molecule can be mapped.
    Additional Material: 10 Ill.
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