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  • 1995-1999  (58)
  • 1985-1989
  • 1980-1984  (60)
  • 1995  (58)
  • 1982  (60)
  • Life Sciences
  • 1
    Electronic Resource
    Electronic Resource
    Non-selective precultivation of bacteria able to degrade different polycyclic aromatic hydrocarbons (PAH) (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 337-345 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of the precultivation with different carbon sources on the ability of three different bacterial strains (Sphingomonas sp. strain BA2, Gordona sp. strain BP9, Mycobacterium sp. strain VF1) to grow on phenanthrene. anthracene, pyrene or fluoranthene as the sole source of carbon and energy were studied. The strains were found to maintain their ability to grow on two of the four PAH after 30 serial transfers in liquid nutrient broth medium without selective pressure. The ability to grow on these PAH as the sole carbon and energy source was also maintained after curing experiments with acridine orange. The high stability of the PAH-degradation phenotype suggests that the tested strains carry at least parts of the PAH-degradation pathway genes on the chromosome. The PAH-degradation versatility of the strains was also influenced by the carbon source being used for precultivation. Possible reasons for the particularly good impact of the precultivation on hexadecane on the PAH degradation are discussed in this paper.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150404
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  • 2
    Electronic Resource
    Electronic Resource
    Masthead (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150401
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  • 3
    Electronic Resource
    Electronic Resource
    The properties of subtilisin, Novo type, immobilized on porous glass (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 123-129 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies of the properties of subtilisin, Novo type, immobilized on porous glass with the aid of hexamethylene diisocyanate were carried out. The immobilized proteinase preparation shows optimum activity at a pH value of 10.7 and at a temperature between 60-65°C. It was stable in a wider range of pH and temperature values than the native subtilisin. The KM values for hemoglobin and BAEE were 9.2 × 10-5 [M] and 139 × 10-5 [M], respectively. Under relatively non-aqueous conditions, immobilised subtilisin was able to synthesize phenylacetic acid ethyl ester.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150117
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  • 4
    Electronic Resource
    Electronic Resource
    The microbial conversion of different agricultural residues and its biological efficiency (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 131-135 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of agricultural residues such as Saccharum munja Roxb. (Sarkanda), Oryza sativa L. (Paddy straw, as the control), Vinna unguiculata (L.) Walp (Cowpeas), Abelmoschus esculentum (L.) Moench (Lady's finger), Zea mays L. (Maize) and Cyamopsis tetragonoloba (L.) Taub. (Guar) were used for the cultivation of Pleurotus sajor-caju (Fr.) Singer. The biological efficiency of the fruit bodies of Pleurotus sajor-caju from the above mentioned substrates were found to be 13.47, 11.20, 8.37, 8.31, 6.87, and 6.08 percent, respectively. S. munja and paddy straw were found to be the best substrates for the growth of P. sajor-caju followed by Z. mays, V. unguiculata, A. esculentum, and C. tetragonoloba.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150119
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  • 5
    Electronic Resource
    Electronic Resource
    Römpp Chemie Lexikon. (Vol. 3-6). Stuttgart, New York: Georg Thieme Verlag 9the edition; DM 1488,- (vol. 1-6). ISBN 3-13-734809-9 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 136-136 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150120
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  • 6
    Electronic Resource
    Electronic Resource
    Masthead (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150201
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  • 7
    Electronic Resource
    Electronic Resource
    Whey disposal by deproteinization and fermentation (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 139-148 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The non-pollutant plant support material of the dwarf duckweed Wolffia arrhiza (Fam. Lemnaceae) was used for the entrapment of living yeast cells (Kluyveromyces fragilis) which hydrolyse lactose with the subsequent fermentation of glucose and galactose at high cell densities (up to 7.0 × 108/ml support). The stabile yeast-plant cell immobilizates are able to produce ethanol from lactose-containing media (e.g. whey) by batch fermentation (on a rotary shaker) or continuous fermentation (in a turbulence reactor) for several days (at a pH below 4.2 and a temperature of 30°C). The removal of whey proteins by a preceding heat denaturation of whey, high dilution rates, CSo values of 50 to 60 g lactose per litre whey and the preferential use of the K. fragilis strain DSM 7238 were determined as the prerequisites for an optimum continuous fermentation. Economically interesting productivities (Pmax ≥ 15 g ethanol/1 · h, D = 0.72 h-1) with an actual lactose turnover of 90% were obtained by using these parameters.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150202
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  • 8
    Electronic Resource
    Electronic Resource
    Grenzwerte. Kennzahlen zur Umweltbelastung in Deutschland und in der EG. Landsberg: ecomed-Verlag GmbH & Co., KG 254 Seiten, DM 98,- (Fortsetzungspreis) DM 178,- (Einzelpreis ohne Abonement der Aktualisierungen). ISBN: 3-609-75700-0 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 160-160 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150204
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  • 9
    Electronic Resource
    Electronic Resource
    Yeast entrapment in Duckweeds: Immobilization performance and biomass localization within the support particles (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 149-159 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cell structured support material (CSM) prepared from Wolffia arrhiza fronds were loaded with Saccharomyces cereevisiae by the propagation of yeast cells introduced in the aerenchyma. The loading process could be strongly accelerated by preventing the growth of freely suspended yeasts in the fermenter. The pathway of the inoculation through the stomata could be visualized by transparent light microscopy of amylase and protease treated CSM. Slices of the loaded CSM clearly showed that the framework of the inner chlorenchyma cells remains intact during the propagation of the included yeast cells. Most of the yeast cells were found to be localized as a dense suspension within unbroken cells. During long-term treatment of the loaded CSM at high convection, about one third of the yeasts was released into water with a constant rate greater than 0.34 h-1. The residual amount of yeast cells remained in a stable compartment from which further loss did not occur for the period tested (20-48 h). It is assumed that the stable compartment is identical with the interior of chlorenchyma cells. The results are discussed in relationship to the possible use of the immobilized biomass in fermentation.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150203
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  • 10
    Electronic Resource
    Electronic Resource
    Fermentation kinetics of free and immobilized Penicillium raistrickii able perform the 15α-hydroxylation of 13-ethyl-gon-4-ene-3,17-dione (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 161-171 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growing Penicillium raistrickii i 477 cells immobilized by microencapsulation, entrapment in calcium alginate beads and photopolymerization were used for the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione (I) to 15α-hydroxy-13-ethyl-gon-4-en-3,17-dione (II). The immobilized cells had lower maximum specific growth rates and yield coefficients when cultivated on the carbon source glucose than the non-immobilized cells, which leads to lower volumetric productivities than the use of nonimmobilized cells. However, the cells immobilized by microencapsulation and calcium alginate entrapment showed a specific productivity equal to that of the respective non-immobilized cells based on product formation per dry biomass and time. Photommobilized cells were not able to grow in the presence of the steroid because the substrate concentrations within the polymer reached inhibiting amounts for growth and product formation. In the absence of the steroid, the growing photoimmobilized cells showed a prolonged lag-phase in comparison with the free cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150205
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  • 11
    Electronic Resource
    Electronic Resource
    Molecular genetics of bacteria. Chichester, New York, Brisbane, Toronto, Singapore: John Wiley & Sons, 1994 287 pages, 140 figures, £ 16.95 ISBN 0-471-95111-0 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 172-172 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150206
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  • 12
    Electronic Resource
    Electronic Resource
    “Eco-Tech Network”. An information, education and research network for ecotechnology Eco-Technology centres - interconnected world-wide (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 196-198 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150208
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  • 13
    Electronic Resource
    Electronic Resource
    “Macroscopic pattern analysis” based on formal analogies as a scientific methodology for complex systems (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 173-195 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bioprocess engineering at present concentrates on the enormous problems in the environment. What is therefore needed is a sound methodology, which should be based on the interactions between the physiology of biological reaction networks and physical processes in the environment and which should be an analogy to bioreactor performance. The conventional methodology is empirically oriented, using pilot plant data for the experimental estimates of process economics, where all further details are elucidated following the mechanistic approach on the microscopic level based on assumed mechanisms (causalities). According to the new view, the new systems-based methodology uses mathematical models as approximations and includes all the interactions. Pilot plant data are needed for model falsification, using analogies on the formal macroscopic level. Bioreactor scale-up as one application is a more rapid procedure of reasonable accuracy, where both the biokinetics as well as the fluid dynamics are quantified using formal macroscopic analogies.Model consistency and plausibility are the basic criteria when using model computer simulations as a decisive aid, while experiments lose their central role and are on longer the basis for evaluating the scientific work; they are simply the basis of the researcher's intuition. Another typical feature of complex systems is that model parameters are interdependent. The final decisive fact will be the mental experiment (“thinking” with the left and right side of the brain), which can be supported using computer simulations. This evolutionary interplay between the three realities of thinking, experimenting and simulating leads to a holistic progress towards the better understanding of highly complex systems. It offers the solution to the problems, which is needed in future.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150207
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  • 14
    Electronic Resource
    Electronic Resource
    Integrated design of a fermentation plant. The production of Baker's yeast. Weinheim, New York, Basel, Cambridge, Tokyo: VCH Verlagsgesellschaft mbH, 1994 XIV, 86 pages, 12 figures, 6 tables; DM 98,-(£ 16.95) ISBN 3-527-30029-5 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 209-210 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150210
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  • 15
    Electronic Resource
    Electronic Resource
    The purification and immobilization of Penicillium notatum β-galactosidase (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 211-222 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Penicillium notatum No. 1 as a producer of β-galactosidase was cultivated in a 5-1 fermenter. Various methods of protein isolation and concentration from the culture fluid were optimized. Then the conditions of β-galactosidase purification using an affinity chromatographic technique were established. The purified enzyme was immobilized on a controlled porous glass (CPG). The optimum temperature and pH values of the native and immobilized forms of β-galactosidase were determined as 50°C and 30-50°C as well as pH 3 and pH 3-5, respectively.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150211
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  • 16
    Electronic Resource
    Electronic Resource
    Masthead (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150101
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  • 17
    Electronic Resource
    Electronic Resource
    Bioprocess engineering. New York, Chichester, Brisbane, Toronto, Singapore: Wiley-Interscience, John Wiley & Sons, Inc., 1994, 338 pages; £ 58. ISBN 0-471-03534-3 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 40-40 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150104
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  • 18
    Electronic Resource
    Electronic Resource
    Intensification of transfer processes in biotechnology and chemical engineering using static mixers. (Review) (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 3-26 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A review of the intensification of moment, mass and heat transfer using an “unconventional” method, namely the application of static mixers, also known as “motionless mixers”, is presented in this paper.After the presentation of some structural types of static mixers, studies with particular emphasis on flow behaviour, pressure drop, mixing, mass and, heat transfer in the presence of static mixing devices, used in biotechnology and chemical engineering, were discussed.The suitability of static mixers to enhance the transfer process parameters was also justified by the low energy requirement, while the fluids might have different viscosities and because of this fact they could remove any mistakes made by the equipment.The article is not directed towards a comprehensive review, but it should serve as a landmark in future undertakings.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150102
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  • 19
    Electronic Resource
    Electronic Resource
    Colonisation of seedling roots by 2,4-D degrading bacteria: A plant-microbial model (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 27-39 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The development of model plant-microbial associations between Gram negative soil microbes capable of degrading phenoxyacetate herbicides, such as 2,4-D and 2,4-D methyl ester, and the crops canola and wheat was described. Both an Acinetobacter baumannii pJP4 transconjugant and Alcaligenes eutrophus JMP 134 colonised non-parasitically on the roots of sterilised seedlings in a hydroponic system. Laser scanning confocal microscopy has shown that colonisation occurred both on the root surface and deeper inside the mucilage layer or inside some surface root cells. When 2,4-D was added to the hydroponic medium supporting the growth of those seedlings colonised by 2,4-D degrading bacteria, the gas chromatographic analysis showed a rapid decrease in the concentration of this herbicide. These bacteria colonising the root system were shown to be responsible for the degradation of 2,4-D. Plants inoculated with the 2,4-D degrading microbes were subsequently found to be less susceptible to damage by the herbicide in such hydroponic systems.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150103
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  • 20
    Electronic Resource
    Electronic Resource
    Growth control and ppGpp synthesis in Brevibacterium flavum cells at various medium mixing rates and aeration intensities (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 41-48 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Under the conditions of various aeration and medium mixing intensities (even at a constant medium oxygen partial pressure value), the variations in the RNA synthesis as well as the growth rate of lysine producing Brevibacterium flavum strains are inversely correlated with the ppGpp concentration in the cells.An increase in the ppGpp synthesis and a decrease in the RNA content in the cells was observed in the cases of a low cell energy charge value (lower than 0.6). This took place in the cases of bacterial cultivation at a low or very high medium aeration and mixing intensity. Hence, the energy production in the cells, the ppGpp synthesis and the growth control mechanism in Corynebacteria may be regarded as connected processes.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150105
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  • 21
    Electronic Resource
    Electronic Resource
    Formation of a micronuclei-like structure induced by colchicine treatment in Saccharomyces cerevisiae (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 49-55 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Minute nuclei named “smaller nuclei” were generated when the cells of Saccharomyces cerevisiae were treated with colchicine. The formation of “smaller nuclei” seemed to be related to nuclear division because those nuclei were only produced under conditions suitable for nuclear division. The fact that the average DNA content of “smaller nuclei” was almost one tenth of that of the isolated normal diploid nuclei showed that the “smaller nuclei” are not condensed nuclei but aneuploid nuclei like micronuclei in animal cells. It appeared therefore likely that a micronuclei-like structure could be produced by colchicine treatment in S. cerevisiae.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150106
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  • 22
    Electronic Resource
    Electronic Resource
    Biotechnology. A multi-volume comprehensive treatise completely revised 2nd edition. Volume 3. Bioprocessing. Weinheim, New York, Basel, Cambridge: VCH Verlagsgesellschaft mbH, 1993. ISBN 3-527-28313-7 (Weinheim). ISBN 1-56081-153-6 (New York). Single volume price: DM 490.00, Series Price: DM 390.00 per volume, Series ISBN 3-527-28310-2 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 55-56 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150107
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  • 23
    Electronic Resource
    Electronic Resource
    Degradation of synthetic lignin by the protoplasts of Phanerochaete chrysosporium in the presence of lignin peroxidase or manganese peroxidase (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 57-66 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lignin was mineralized in the experiments in which 14C-lignin was incubated with lignin peroxidase or manganese peroxidase in a tartrate buffer in the presence of cycloheximide-treated protoplasts obtained from the ligninolytic mycelia of Phanerochaete chrysosporium. The rate of lignin mineralization was dependent on the lignin peroxidase or manganese peroxidase concentration in the medium. In the experiments in which lignin was incubated with lignin peroxidase or manganese peroxidase, lignin was repolymerized irrespective of the presence of protoplasts mineralizing lignin, suggesting that an active degradation of lignin and repolymerization took place. Taking into account that lignin peroxidase and manganese peroxidase were the only extracellular enzymes in the experiments in which lignin was mineralized by the protoplasts, it is postulated that lignin peroxidase and/or manganese peroxidase can degrade lignin into small fragments which can then be further absorbed by the fungal cells and subsequently degraded to CO2.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150108
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  • 24
    Electronic Resource
    Electronic Resource
    Behaviour of different Lactobacillus species in the production of lactic acid by the fermentation of whey (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 67-76 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The behaviour of different Lactobacillus casei and Lactobacillus plantarum species in the fermentation of Manchego whey was experimentally studied and the results were statistically analyzed using a hypothesis contrast method. The steadiness of the velocity of the production of lactic acid during the fermentation process allowed the use of this variable to compare the different microorganisms. From this comparison it was inferred that the individuals of the same population behave alike and that the L. casei population produces lactic acid at a higher rate than the L. plantarum population. A competitive effect among the members of the L. casei population was also observed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150109
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  • 25
    Electronic Resource
    Electronic Resource
    Monoterpenol glycosides in plants and their biotechnological transformation (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 77-95 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The present paper reviews the different monoterpenols and monoterpene polyols identified in the glycoside form in the plant kingdom. The glycosidic moieties involved are also considered. The natural pathways for the synthesis and hydrolysis of these monoterpene glycosides and the different hypothesis concerning their metabolism are discussed. The present state of different projects for the biotechnological transformation of these aromatic precursor compounds is reported.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150110
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    Electronic Resource
    Bioprocess engineering. Biotechnology and microbiology. Cambridge, New York, Melbourne: Cambridge University Press, 1994, 293 pages, 100 figures, 50 tables; £ 58,-(H/b). ISBN 0-521-35020-4 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 96-96 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150111
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  • 27
    Electronic Resource
    Electronic Resource
    Glucose oxidase biosynthesis in relation to biochemical mutations in Aspergillus niger (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 107-115 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The production of extracellular glucose oxidase in a submerged culture by a number of auxotrophic, 2-deoxy-D-glucose resistant and protease-less mutants of Aspergillus niger was evaluated. Among the auxotrophic strains, no evident dependence was found between the kind of the nutritional requirements and the level of the glucose oxidase activity. However, the majority of auxotrophs, requiring serine or niacin, showed a higher enzyme activity (from 16 to 680%) than the parent strain. The dynamics of the glucose oxidase synthesis by the free and immobilized mycelium of the most active niacin- mutant of A. niger was also investigated.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150113
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  • 28
    Electronic Resource
    Electronic Resource
    Cloning and expression of the Neisseria meningitidis 5C outer membrane protein (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 97-106 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The 5C outer membrane protein, one of the N. meningitidis class 5 proteins, was preferably expressed in bacteria isolated from the nasopharynx and its role in adhering to the mucosal cells and invading them as well as the development of anti-5C antibodies in healthy carriers was demonstrated. Anti-5C monoclonal antibodies are bactericidal in the presence of the human complement. The immunodominant region of the 5C protein is highly conserved among the different strains of N. meningitidis, and the opc gene, which encodes the protein, does not seem to show antigenic variations.Here the isolation of the opc gene from the Cuban strain B:4:P1.15 by PCR (Polymerase Chain Reaction) is presented. Under the regulation of the tryptophan promoter, the gene was cloned and sequenced in E. coli with a high level of expression and fused to the amino-terminal end of the interleukin-2 gene. In the dot-blot experiments, the presence of the gene in those strains which did not express the protein in the whole cell ELISA was also detectable.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150112
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  • 29
    Electronic Resource
    Electronic Resource
    Production of thiaminase I for analytical purposes (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 117-121 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple medium enhancing the production of thiaminase I (EC 2.5.1.2) by Bacillus thiaminolyticus was developed. Ca2+ stimulated the enzyme production. The activity of extracellular thiaminase I ranged between 1.29 and 1.33 U/ml medium.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150115
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  • 30
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    Electronic Resource
    Ologosaccharides: Production, properties and applications. (Japanese Technology Reviews. Section E: Biotechnology. Volume 3, number 2). Switzerland, Australia, Belgium, France, Germany, Great Britain, India, Japan, Malaysia, Netherlands, Russia, Singapore, USA: Gordon and Breach Science Publishers, 1993 XVIII + 235 pages, 120 figures, 62 tables; $ 167.00. ISBN 2-88124-890-X (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 116-116 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150114
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  • 31
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    Electronic Resource
    Principles and techniques of practical biochemistry. Cambridge, New York, Melbourne: Cambridge University Press, 1994 586 pages, 212 figures, 45 tables; £ 19.95 ISBN 0-521-42809-2 paperback (fourth edition) (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 122-122 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150116
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  • 32
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    Electronic Resource
    Genetic engineering of microorganisms. VCH Verlagsgesellschaft mbH: Weinheim, New York, Basel, Cambridge, Tokyo, 1993, 178 pages, 51 figures, 17 tables; DM 38. ISBN 3-527-30039-2 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 130-130 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150118
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  • 33
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    Electronic Resource
    Advances in biosensors. London (England), Greenwich (Conn., USA): JAI Press Ltd. Volume 1, XII + 299 pages, 141 figures, 25 tables; £ 58,-; ISBN 1-55938-240-6 Volume 2, XII + 343 pages, 148 figures, 8 tables; £ 58,-; ISBN 1-55938-270-8 Supplement 1, “Chemical Sensors for in vivo and in vitro Monitoring”, XI + 265 pages, 31 figures, 32 tables; £ 58,-; ISBN 1--55938--630--4 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 287-288 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150306
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  • 34
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    Electronic Resource
    Continuous semi-solid cultivation for the production of cellulase by Trichoderma reesei mutants using a polyurethane foam carrier and a liquid medium (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 289-296 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The production of cellulase was investigated in semi-solid state culture using the immobilized mycelium of Trichoderma reesei mutants on polyurethane foam impregnated with lactose medium. An extremely high value of about 2.6 FPU/ml was reached after the cultivation of T. reesei D-78085 on a 0.5% lactose medium in continuous culture at a pH medium of 4.0 when a bioreactor with vertical polyurethane foam plates was used. The enzyme yield on lactose was 520 FPU/g of lactose metabolized in comparison with 160 FPU/g using a stirred tank bioreactor.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150307
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  • 35
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    Electronic Resource
    The molecular cloning and sequencing of the nitrilase gene of Rhodococcus rhodochrous PA-34 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 297-306 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nitrilase of Rhodococcus rhodochrous PA-34 catalyzes the production of optically active amino acids from aminonitriles. The amino acid sequence of the NH2 terminus of the purified nitrilase was determined for the preparation of a synthetic oligonucleotide as a southern hybridization probe. A 9.5-kbp Pst I-fragement, which hybridized with the oligonucleotide probe, was isolated from R. rhodochrous PA-34 genomic libraries constructed in pUC 19. Nucleotide sequence analysis revealed that the nitrilase gene codes for a putative polypeptide of 380 amino acids which correspond to a relative molecular weight of 41, 723.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150308
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  • 36
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    Electronic Resource
    Cultivation of a filamentous mould in an airlift bioreactor (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 323-335 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cephalosporin C biosynthesis was investigated in a pilot-plant external-loop airlift bioreactor for evaluating the capacity of this bioreactor to surpass the problems which arise from the morphology of the mould and the rheology of the broth.Some of the results were compared with those obtained in a stirred tank bioreactor.The dilution and the use of static mixers was necessary to overcome the effects of the high viscosities. The oxygen transfer rate represented 84% of that in the stirred bioreactor, but the efficiency of the power utilization was higher.The specific productivity of Cephalosporin C is comparable to that obtained in the stirred tank bioreactor, but the average specific power consumption was found to be 2/3 of that in the stirred vessel.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150402
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  • 37
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    Electronic Resource
    Methoden der Biotehnologie. Jena, Stuttgart: Gustav Fischer Verlag, 1994 291 pages, 53 figures, 32 tables; DM 78,-. ISBN 3-334-60836-0 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 346-346 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150405
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  • 38
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    Electronic Resource
    Studies of the mixing character and flow distribution in mycelial fermentation broths (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 355-366 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of two mixing systems on the principal parameters of mycelial fermentations of Aspergillus niger, Fusicoccum amygdali Del. and Fusarium moniliforme Sheld. as well as their metabolite citric acid, fusicoccin and gibberellic acid production was analyzed from the viewpoint of flow energy distribution in a bioreactor.The growth and metabolite synthesis during fermentation was compared under different mixing conditions in the fermenter FU-8 with a turbine mixing system (TMS) and a counterflow mixing system (CMS). It was found that the growth, productivity and respiration characteristics as well as the morphology of these cultures varied dependent on the mixing system and agitation regime used. The counterflow mixing system was more favourable for large agglomerates (F. amygdali) or soft pellets (A. niger) forming fungi, while the turbine mixing system was more effective for F. moniliforme growing in the form of small clumps and freely dispersed hyphae. Flow characteristics under different mixing conditions were analyzed in a model fermenter.The kinetic energy of flow fluctuations was measured in gassed and ungassed water and different fermentation broth systems by using a Stirring Intensity Measuring Device (SIMD-F1). The difference of the energy values at different points was better expressed in the fermenter with a turbine mixing system in comparison with that having a counterflow mixing system. High viscous F. amygdali and A. niger broth provided higher energy values compared to water and low viscous F. moniliforme broth. It was observed that the intensity of growth and the intensity of the synthesis decreased at very high energy values, which was obviously connected to the influence of the irreversible shear stress on the mycelial morphology.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150408
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  • 39
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    Electronic Resource
    Improved ethanol production from whey with Saccharomyces cerevisiae using permeabilized cells of Kluyveromyces marxianus (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 387-390 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Permeabilized cells of Kluyveromyces marxianus CCY eSY2 were tested as the source of lactase in the ethanol fermentation of concentrated deproteinized whey (65-70 g/l lactose) by Saccharomyces cerevisiae CCY 10-13-14. Rapid lactose hydrolysis by small amounts of permeabilized cells following the fermentation of released glucose and galactose by S. cerevisiae resulted in a twofold enhancement of the overall volumetric productivity (1.03 g/l × h), compared to the fermentation in which the lactose was directly fermented by K. marxianus.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150413
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  • 40
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    Electronic Resource
    Stability of the pBR322 plasmid derivative pBB210 in Escherichia coli TG1 under non-selective and selective conditions (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 375-380 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The stability behaviour of the pBR322 plasmid derivative pBB210 with β-lactamase gene and human interferon-α1 gene in Escherichia coli TG1 was studied in chemostat cultures under non-selective (medium without antibiotics), selective (medium with β-lactam antibiotic ampicillin) and modified selective (medium with ampicillin and the β-lactamase inhibitor sulbactam) conditions. Under non-selective conditions, a behaviour typical of unstable systems was found. Under selective conditions, the behaviour predicted by the models was obtained - the fraction of plasmid-bearing cells in the population approached a constant value which was dependent on the ampicillin concentration in the feeding and on the cell concentration in the chemostat. Under modified selective conditions, the higher the concentration of sulbactam in the medium was, the higher the fraction of plasmid-bearing cells was in steady state conditions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150411
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  • 41
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    Electronic Resource
    Techniques and instrumentation in analytical chemistry (volume 16) flow - through (bio) chemical sensors. Amsterdam, London, New York, Tokyo: Elsevier Science B.V., 1994 332 pages; Dfl. 355.00, US$ 202.75 ISBN 0-444-89866-2 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 267-268 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150303
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  • 42
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    Electronic Resource
    Changes in protein secretion of Aspergillus niger caused by the reduction of the water activity by potassium chloride (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 277-287 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of the rapid reduction of the water activity (aw) on the extracellular protein and amylolytic activity of Aspergillus niger was studied. An aw value gradient from 0.90 to 0.99 in KCl solutions was applied for the mycelium treatment. It was found that the aw reduction considerably influenced the protein secretion. This phenomenon was dependent on the age of the treated mycelium and the range of the aw gradient. The highest protein and enzyme secretion yields were obtained at aw = 0.98 using a 72-h old mycelium. In comparison with the non-treated mycelium, the increase in the secretion amounted to about 60% for the amylolytic activity and 37% for the soluble protein, respectively. It was shown that the mycelium incubated in KCl solutions of an aw value from 0.90 to 0.99 had the ability for regeneration in fresh CZAPEK-DOX medium. The effect of the osmotic shock on the protein secretion was limited only for the treated cell population and declined in the mycelium which was regenerated after the transfer into the culture medium.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150305
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  • 43
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    Electronic Resource
    Characterization of biosurfactants and their use in pollution removal - State of the Art. (Review) (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 251-267 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Surface-active compound of biological origin (biosurfactants) have only been described in the past few decades. With the advantage of biodegradability and production on renewable resources, biosurfactants have been gaining prominence and their applications are becoming wider. So far, literature contains mixed reports on the successes of the applications of biosurfactants and their economical viability. They remain compounds which are not very well understood, yet, with several important applications. The target industries for biosurfactant use are the petroleum remediation industries and environmental conservation agencies. These industries, however, seem reluctant to use them for fear of dealing with microbes or microbial products. This includes cleaning up oil spills from the environment, remediation of metal-contaminated soils or waste streams, mobilizing heavy oil sludge and enhanced oil recovery. The importance of biosurfactants, their production, characteristics and limited successes and applications in oil pollution remediation and oil storage tank cleaning are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150302
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  • 44
    Electronic Resource
    Electronic Resource
    Capacities and limits of three different technologies for the biological treatment of leachate from solid waste landfill sites (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 269-276 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Leachate from a municipal waste landfill site was treated using an activated sludge bioreactor, a fluidized bed biofilm reactor and a packed-bed column reactor (trickling filter).The leachate contained high organic matter (2.0-2.6 g/l of COD), high ammonium (300-700 mg/l) and sulphide (200-800 mg/l) concentrations, as well as low metal concentrations.The continuously operating reactors were employed to study the effects of TOC loading on the removal of TOC as well as on the nitrification and denitrification processes.Among the three biological treatment technologies investigated, the fluidized bed biofilm reactor was best with respect to removing ammonia and TOC. More than 90% of TOC and 99% of ammonia were removed when TOC loading was less than 0.5 kg/m3 × d. At a TOC loading of 4 kg/m3 × d, the removal of TOC and ammonia was 80% and 99%, respectively.In contrast, the treatment of leachate with the packed-bed reactor was successful in TOC removing only at TOC loading less than 0.3 kg/m3 × d (TOC elimination decreased from 86% at 0.06 kg/m3 × d to 60% at 0.3 kg/m3 × d). However, the reactor was active in nitrification even at a higher TOC loading (more than a 98% ammonia elimination at a TOC loading of 0.5 kg/m3 × d).Leachate was processed in the activated sludge reactor when TOC loading was less than 0.5 kg/m3 × d (with a removal of TOC and ammonia up to 83% and 99%, respectively). The activated sludge reactor was also effective in TOC removal at a higher TOC loading (e.g. a 74% TOC removal at a TOC loading of 1 kg/m3 × d), but for ammonia elimination, the activity continuously decreased (less than 60% ammonia removal at a TOC loading of 1 kg/m3 × d).Overloading in the activated sludge system was indicated by a high concentration of ammonia and nitrite in the effluent. In the packed bed reactor, overloading was characterized by a progressively incomplete TOC removal. No significant overloading was found in the fluidized bed reactor up to a TOC loading of 4 kg/m3 × d.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150304
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  • 45
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    Electronic Resource
    Biological reaction engineering. Principles, applications and modelling with pc simulation. Weinheim, New York, Basel, Cambridge: VCH Verlagsgesellschaft, 1992 438 pages, DM 228,-ISBN 3-527-28511-3 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 317-318 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150310
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  • 46
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    Electronic Resource
    Studies on the root nodules of leguminous plants: The production of indole acetic acid in culture by a Rhizobium sp. from a leguminous climbing shrub Derris scandens benth (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 307-317 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The Rhizobium sp. When isolated form the root nodules of a leguminous climbing shrub Derris scandens produced a high amount of indole acetic acid (IAA) (135.2 μg/ml) from the tryptophan-supple-mented basal medium. Growth and IAA production started simultaneously, and the maximum amount of IAA was produced as a secondary metabolite in the stationary phase of growth. The IAA production by the Rhizobium sp. was increased by 503% when the medium was supplemented with mannitol (2%), KNO3 (0.2%), nicotinic acid (0.1 μg/ml) and MnSO4 (1 μg/ml) in addition to tryptophan (4 mg/ml)/The possible role of the rhizobial production of IAA on the rhizobia-legume symbiosis is also discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150309
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  • 47
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    Electronic Resource
    Flow cytometry: New developments NATO ASI series, Series H, cell biology, volume 67. Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong: Springer-Verlag 473 pages, 158 figures, 12 tables; DM 348,- ISBN: 3-540-54606-5 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 335-336 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150403
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  • 48
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    Biosensoren. Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong: Springer-Verlag, 1995 417 pages, 181 figures, 24 tables; DM 49.80. ISBN 3-540-57478-6 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 354-354 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150407
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  • 49
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    Determination of the Ks values during the growth of Alcaligenes eutrophus on phenol, 2,4-dichlorophenoxyacetic acid and fructose (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 347-353 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The determination of the KS values presented here is based on the estimation of the stationary substrate concentrations in continuous cultivation experiments. The separation of biomass from the suspension was performed by an ultrafiltration step which succeeded within one second. The decay of substrate concentration during sampling was calculated to amount to less than 6% of the stationary substrate concentration at relevant growth rates. The KS values derived from these reduced substrate concentrations deviated by only 10% from the theoretical values at a biomass concentration of about 1 g/1. Thus relevant kinetic parameters can be calculated from the data obtained by this procedure. Values of 11, 59 and 14 μM were obtained with 2,4-dichlorophenoxyacetic acid (2,4-D), phenol and fructose, respectively. Similar KS values were derived with 2,4-D and fructose by using a respirationbased determination for reasons of comparison. With phenol this value was only 7 μM which is as cribed to a physiological background.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150406
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  • 50
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    Biotechnologie in der Lebensmittelindustrie. Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong: Springer-Verlag XVII + 241 pages, 54 figures, 24 tables; DM 49.80 ISBN 3-540-57452-2 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 374-374 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150410
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  • 51
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    Physiological restriction of the L-lactic acid production by Rhizopus arrhizus (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 367-374 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of basic physiological factors on the quality of inocula and L(+)-lactic acid production by Rhizopus arrhizus CCM 81 09 were studied.The most effective preparation of the spores (5 × 107 spores/ml) and subsequent good lactate production was achieved on the agar medium with soil extract and malt agar. The optimum initial amount of active spores for inoculation was 103-104 spores/ml. The preparation of inoculum required intensive stirring with lower aeration and pH maintained in the range from 4.8 to 6.0 by the addition of CaCO3. The maximum yield of lactic acid production was achieved by using 5% (v/v) of 24-h-old inoculum. The intensity of lactic acid production in the inoculum was proportional to its production in the subsequent steps of fermentation and can be used as a fast control of the physiological state of the producers.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150409
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  • 52
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    Effect of oleic acid on the production of ethanol and fructose from glucose/fructose mixtures in an immobilized cell reactor (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 381-386 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Saccharomyces cerevisiae ATCC 39859 was immobilized onto small cubes of wood to produce ethanol and very enriched fructose syrup from glucose/fructose mixtures through the selective fermentation of glucose. A maximum ethanol productivity of 21.9 g/l-h was attained from a feed containing 9.7% (w/v) glucose and 9.9% (w/v) fructose. An ethanol concentration, glucose conversion and fructose yield of 29.6 g/l, 62% and 99% were obtained, respectively. This resulted in a final fructose/glucose ratio of 2.7. At lower ethanol productivity levels the fructose/glucose ratio increases, as does the ethanol concentration in the effluent. The addition of 30 mg/l oleic acid to the medium increased the ethanol productivity and its concentration by 13% at a dilution rate of 0.74 h-1.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150412
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  • 53
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    ACS symposium series 516: Biocatalyst design for stability and specificity. Washington, DC: American Chemical Society 335 pages, $ 89.95, ISBN 0-8412-2518-4 (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 232-232 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150213
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  • 54
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    The effects of xylanase type enzymes on the different layers of birch wood ORGANOSOLV pulp fibre walls (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 199-209 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some effects of the xylanase treatment on the separate birch ORGANOSOLV pulp fibre wall morphological layers were examined. These investigations were focused on the outer layers, i.e. the primary wall (P) and the outer layer of the secondary wall (S1), as well as the central layers, i.e. the central layer of the secondary wall (S2) and the tertiary wall (T). Step by step, the fractionation of the pulp components in the polar solvents N,N-dimethylformamide (DMFA), dimethylsulphoxide (DMSO) and DMSO/H3PO4 was used as a mild technique for the isolation of the lignin-carbohydrate complexes. The different residual amounts of lignin and hemicelluloses in the outer and central pulp fibre wall layers as well as the different lignin-hemicellulose ratios were determined. The size-exclusion chromatographical (SEC) analysis showed a higher initial lignin content in the region of the high molecular mass (MM) fibre wall fraction extracted with “DMSO/H3PO4” than the outer cell wall layers. In the central layers, the amounts of soluble lignin (calculated on the mass of total dissolved substance) were approximately the same for all the three solvents.The xylanase treatment brought the most considerable changes in the high MM part of the residual lignin (the lignin carbohydrate complex). This was true for both the P-S1 and S2-T layers. The careful brightness comparison of the outer and central fractions after the X-E-P-P bleaching sequence showed a surprisingly low bleachability of the outer layer fraction. The xylanase action depended on the composition of the lignin-carbohydrate complex (LCC) and the extent of the maintenance of the outer layers during the pulping process.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150209
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  • 55
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    Characterization of casein peptones by HPLC profiles and microbiological growth parameters (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 223-231 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Thirty six pancreatic casein peptones from various producers were analyzed by HPLC and by microbiological growth tests. The data were compared by using the principal coordinate analysis and simple mathematical tests. The Proteose peptone quotient, PQ1, determined by the growth of Escherichia coli, separated these casein peptones into three groups, depending on their suitability as nutrients compared to Proteose peptone as a standard. These groups corresponded to the HPLC patterns, characterized by the specific peaks of polypeptides, peptides and amino acids, respectively. The peaks of the high-molecular weight polypeptides were negatively correlated with the growth parameters. The peptides of a low molecular weight promoted the growth of Staphylococcus aureus with and without the exhaustion of usable N compounds and in the presence and absence of glucose (PQ2 and PQ3). The colony sizes correlated weakly with certain peaks only. HPLC patterns, thus, seem to be useful for the characterization of casein peptones for the standardization of nutrient media as well as for special applications.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150212
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  • 56
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    Production of α-glucosidase by Bacillus sp. strains (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 233-240 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: α-Glucosidase was detected in four wild-type amylolytic strains belonging to the Bacillus genus. The strains showed α-glucosidase activity in extracellular and membrane-bound fractions. Kinitic studies of the α-glucosidase synthesis in the batch cultures of four strains of the Bacillus genus showed two profiles: partially and totally growth-linked synthesis.The presence of different activities and production profiles of α-glucosidase in the strains at high or low glucose concentrations in the medium would indicate that α-glucosidase may have a role in the regulation of the metabolism of α-polysaccharides.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150214
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  • 57
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    Masthead (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150301
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  • 58
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    Production of rifamycin B and SV by free and immobilized cells of Amycolatopsis mediterranei (1995)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 15 (1995), S. 241-248 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of growth conditions (incubation time, inoculum size, initial pH value) and some nutrient concentrations on the growth and rifamycin B and SV production by free and immobilized cells of Amycolatopsis mediterranei CBS 42 575 was studied. In alginate beads, the immobilized cells behaved like the free cells, but a pronounced difference was observed in antibiotic production and cell growth. The rifamycin production by the immobilized cells was higher than that obtained by the free cells. The immobilized cells were also reused repeatedly for six batch cultivations with a fresh medium charged into flasks at the beginning of each batch. It was found that the immobilized cells were stable, and the rifamycin yield was almost constant during the first three batches and then decreased.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370150215
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  • 59
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    „Biotechnology of microbial synthesis“. Riga 1980, Sinatne publishing house, 350 pp, 74 fig., 59 tab., 582 ref. (Russian) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 42-42 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020103
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  • 60
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    Russian Article pp. 51-58 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 51-58 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper considers techniques of measurement of the curves of oxygen consumption by microorganisms. The widely applied method of obtaining the value of the critical oxygen concentration (COC) using these curves has been analysed. The experimental conditions necessary for the adequate measurement of the culture respiration rate in a fermenter have been found. It has been shown that in the case when the respiration rate within the considered range of pO2 is determined by one and the same enzyme, the COC value is not an apropriate characteristic of the mode of the respiration rate dependence on oxygen concentration.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020105
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  • 61
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    Patents reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 192-192 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020214
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  • 62
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    Immobilization of liver microsomal cytochrome P-450 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 187-191 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Rabbit liver microsomal cytochrome P-450 was immobilized by entrapment in calcium alginate gel. Aminopyrine demethylation experiments showed that the immobilized enzyme system is highly active and exhibits an unimpaired functional stability as compared with crude microsomes. The alginate entrapped microsomes were employed in a fixed bed recirculation reactor, where aminopyrine was continuously demethylated. Such model enzyme reactor can be a useful tool for studying extracorporeal drug detoxification or preparative substrate conversion with microsomal enzyme systems.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020213
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  • 63
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020301
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  • 64
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    Das Autoselect-System - ein Automatensystem zur Selektion von Antibiotikaproduzenten, VII. Hemmhofmeßautomat (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 193-198 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The zone reader of the Autoselect-system enables to measure the 64 inhibition zones of a quadratic bioassay plate with an accuracy of 0,1 mm within 3 minutes. A carriage moves each zone in the light beam. With a special photometrical device 3 defined areas of the inhibition zone can be measured quantitatively by 2 receivers. The indicated values of the diameters result from the analog treatment of the signals. By coupling to a computer the datas of the measuring and other desired informations are printed out.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020215
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  • 65
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    Das Autoselect-System - ein Automatensystem zur Selektion von Antibiotikaproduzenten VIII. Zur Effektivität des Automatensystems (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 199-204 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The advantages of the Autoselect-system are explained. They are offered by a higher speed of all manipulations, by a significantly better accuracy, by getting more informations and by easier physical labour. In the most cases the automated work can be done in a fourth till a tenth of the time needed for manual handlings. The capacity of the machines amounts 5 000 to 30 000 colonies/samples per day. Special problems as well as possibilities for a further increase of the efficiency are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020216
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  • 66
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    Man-made microbes and man-made lawBased on a lecture given at a seminar for representatives of the Academy of Finland and the Academy of Sciences of the G. D. R. in Jena, Dec. 1980. (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 207-212 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The Supreme Court of the US in 1980 granted a patent for a strain of Pseudomonas containing two plasmides after genetic manipulation. This is the first case of patenting a living organism. Whereas the patent law of US and of many other countries further more on supports the principle that natural products are not patentable man-made microorganisms on the other hand fullfil very important crucials of patentability as far as they are new, unobvious, reproducible and useful. The situation arising as the result of this decision is described and the implications and consequenses for the patent law, the taxonomy and the general biological thought are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020302
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  • 67
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    Immobilized enzymes for food processing. Boca Raton, Florida; 1980; CRC Press, Inc. 220 Seiten; 73.95 $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 226-226 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020304
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  • 68
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    Russian Article pp. 213-225 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 213-225 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of assimilation of hydrocarbons having a different structure by Candida yeasts was studied.The rates of oxidation of various carbon atoms in the molecules of isoalkanes, alkylaromatic hydrocarbons and n-alkanes were evaluated in the range of C11 to C28.Metabolic inhomogeneity of carbon atoms in the molecules of isolakanes and alkylaromatic hydrocarbons was observed. A competition in the assimilation of the called hydrocarbons and n-alkanes and also a competition in the assimilation of n-alkanes of a different molecular weight, i.e. metabolic inhomogeneity of carbon atoms of n-dodecane and n-tetracosane was found out for yeasts.
    Additional Material: 11 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020303
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  • 69
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    Modeling step function responses in continuous fermentation processesPaper presented, in part, at the Second Chemical Congress of the North American Continent/180th National Meeting of the American Chemical Society, Division of Microbial and Biochemical Technology, Las Vegas, Nevada, August 28, 1980. (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 227-238 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Understanding both the qualitative and quantitative transient responses to inlet flow and substrate step functions has been a relatively unsolved problem for continuous fermentation processes. This study of transient responses and simple descriptive models suggests that the saturation constant of the MONOD equation is a variable. Thatf variable depends on the rate constants of parallel biochemical pathways leading to cell growth, the concentration of cells themselves, and the concentration of all products in the culture. Mechanisms for describing the hysteresis behavior of the growth rate funcion are postulated in terms of the underlying molecular biology of the microbial system.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020305
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  • 70
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    Ein mikrorechnersystem für fermentationseinrichtungen (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 239-249 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For several years now, we can notice efforts in increasing the efficiency of microbial processes by means of more intensive scientific investigation of such processes. Essential prerequisites to it are improved possibilities for process monitoring (available sensors) as well as facilities for realtime processing of process information. In this paper a microcomputer system is represented, which has been constructed on the base of computing needs for fermentation. The computing needs for fermentation experiments are outlined and the structure of the microcomputer is described. As an example it is illustrated what tasks can be solved by this microcomputer in coupled operation with a laboratory fermentor.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020306
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  • 71
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    Lecture Notes in Biomathematics, Vol. 41., Modèles Mathèmatiques en Biologie, Springer-Verlag, Berlin/Heidelberg/New York, 1981, 219 Seiten, 29 Abb., 28, 50 DM; 13.00 US $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 250-250 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020307
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  • 72
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    Rechnergestützte führung von fermentationsprozessen Teil I (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 251-262 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The industrial continuous fermentation for the production of microbial protein is like any real process subject to disturbances. The considerable social expenditures involved in production make it necessary to restrict the negative consequences of these disturbances to a minimum by means of suitable measures. One such measure is the computer-aided adaptation of the static optimum. For the reaction on nonmeasurable disturbing inputs an algorithm is given containing the steps data filtering, adaption of process model and optimization, and the solution of the data-filtering problem in the widest sense by spline functions is discussed. The application of this algorithm to a specific problem of process control is demonstrated in [1].
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020308
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  • 73
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    Beitrag zur Optimierung des mechanischen Aufschlusses von Hefezellen (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 263-274 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of technological variables on the cell disintegration of yeast suspensions by means of a ball mill and a high pressure homogenizer has been studied by measuring the electrical conductivity. The rate constants and half-life periods are calculated. Regarding the production of protein isolates, the stipulation of optimal disintegration conditions requires a compromise between a degree of disruption as high as possible and a low destruction of the cell walls.By homogenizing, fragments of cell walls arise which are more uniform and better separable in comparison with the milling process. Therefore the mechanical breakage of yeast cells on a large scale should be carried out by the use of high pressure homogenizers.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020309
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  • 74
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    Wasseraufnahme und enzymfreisetzung im getreidekorn (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 297-298 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020312
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  • 75
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    Untersuchungen zum Anfangsstadium der enzymatischen Hydrolyse unterschiedlich vorbehandelter Linterscellulose (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 275-286 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Starting from cotton linters cellulose modified in physical structure type of lattice, degree of order, state of swelling by different pretreatments, and from culture filtrates of Gliocladium spec., the initial stage of enzymatic hydrolysis of cellulose has been investigated. Especially with substrates of high degradability a considerable effect of stirring on rate of formation of soluble products was found. For linerization of yield-vs-time-curves (% residue resp. 7percnt; solubles as a criterion for yield), a 2-parameatric first order rate law was found to be suitable within a limited time interval, values of k1 were higher and for the accessible part of the substrate were lower in the initial stage of hydrolysis than in the later one. The MICHAELIS-MENTEN-constant kM has been determined for substrates of different physical structure after different times of reaction. Data found for kM indicated a stronger dependence of kM on reaction time than on physical structure of the substrate under conditions applied.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020310
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  • 76
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    Bestimmung von Substratverbrauchskoeffizienten für Paraffinkohlenwasserstoffe (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 299-302 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The empirical determined constant of 3.14 gram biomass per available electron is a good base for the calculation of minimum substrate consumption coefficients in the aerobic fermentation of paraffins by yeasts.The analysis of experimental determined specific substrate consumption coefficients and their comparison with the corresponding theoretical values show that the theoretical ones can be reached only if the substrate composition referring to carbon and hydrogen is of optimal composition for all syntheses.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020313
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  • 77
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020401
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  • 78
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    „Ernährungslehre und diätetikldquo;, bd. I, Teil 1 und 2. GEORG THIEME Verlag Stuttgart-New York 1980, 625 Seiten, Teil 1 - 40 Abb., 42 Tab., Teil 2 - 148 Abb., 90 Tab., Preis jeweils 248,- DM (Summe 496 DM), diverse Lit,-Zit. (kapitelweise) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 303-303 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020314
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  • 79
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    Biotechnology. Verlag Chemie, Weinheim; Deerfield Beach, Florida; Basel, 1981, Volume 1: Microbial Fundamentals, ca. 550 Pages, 247 Figures, 106 Tables, DM 495,-; Subcriptionprice DM 425,- (if taking all volumes), Time for subcription up to June, 30, 1982 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 304-304 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020315
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  • 80
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    Biotin vitamers content of lactose and beet molasses (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 369-375 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The biotin activity of beet and lactose molasses against the test strain Saccharomyces cerevisiae 225 by auxanographic method was evaluated. The level of lactose molasses biotin activity is almost twice as high as that obtained in the case of beet molasses. The results of bioautography with test strains Saccharomyces cerevisiae 225 and Lactobacillus arabinosus 17-5 indicate the qualitative composition of biotin derivatives (vitamers) in both molasses. Depending on the various technological steps e.g. sterilization or clarification one may find differences in the content and qualitative composition of biotin vitamers.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020409
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  • 81
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    Masthead (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020101
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  • 82
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    Status and developments of animal cell technology using suspension culture techniques (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 3-41 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In this review the state of the art in animal cell technology, using suspension culture techniques is updated as far as the end of 1980. We have tried to discuss, on a broad basis, the current status and potential developments of both, the purely biological and the biochemical engineering aspects which may be important to improve the performance and design of animal cell technologies. The process economics could be considerably improved by the use of transformed animal cell substrates, the use of cheaper cultivation media and by methodological and engineering means. Most of these aspects are in the state of realization. Nevertheless, for a great variety of biological active substances which do not require so - or posttranslational processing, recombinant DNA-techniques (e.g. genetic engineering) are a promising alternative for the production of animal cell derived substances.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020102
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  • 83
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    Enzyme engineering, vol. 5. New York-London, 1980, Plenum Press, 491 Seiten; 49.50 $ (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 72-72 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020107
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  • 84
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    Grundlagen der Verfahrenstechnik. Springer-Verlag Wien/New York 1981 377 Seiten 138 DM (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 78-78 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020109
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  • 85
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    Die Anwendung von Produkten der mikrobiologischen Synthese in der Tierproduktion. Verlag „Kolos“, Moskau, 1980, 288 Seiten, 112 Tabellen und Übersichten (in russischer Sprache) (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 86-86 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020111
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  • 86
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    Gewinnung und Charakterisierung von Prolidase aus, Escherichia coli B., I. Gewinnung des Enzyms (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 87-94 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: An enzyme being able to hydrolize the imido linkage at the N-terminal end of proline is isolated from E. coli B. This fact corresponds to the specifity of hydrolization of the animal prolidase. Enzyme synthesis within the cells of E. coli B is carried out independently from growth. Changed environmental factors may influence the formation rate of enzyme in a restricted way. A relatively high enzyme biosynthesis can be reached by cultivating the strain E. coli B at a temperature of 37°C as well as an initial pH-value of the medium of 7.0 in submerged culture (400-500 rpm) By variation of the medium composition enzyme synthesis does not change considerably, however, biomass yield can be increased about 100% If mechanical cell desintegration is optimized by means of ultrasonic or vibration homogenisator the cell components may be easily released with a higher proline specific activity as animal prolidase.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020112
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  • 87
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 114-114 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020116
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  • 88
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 115-115 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020117
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  • 89
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    Patent Reports (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 116-116 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020118
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  • 90
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    Regulation der mikrobiellen Alkanoxidation mit Hinblick auf die ProduktbildungVortrag, gehalten anläßlich des 2. Symposiums der sozialistischen Länder über Biotechnologie, Leipzig, 2.-5. 12. 1980 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 127-138 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Catabolic pathways of long-chain n-alkanes in the range of C8 to C18 are demonstrated and results of investigation about the regulation of monoterminal oxidation are given: - Enzymes of monoterminal alkane oxidation usually are inducible- Several intermediates of alkane oxidation can inhibit the primary oxidation of concerned alkane-Substances, e.g. glucose and glycerol, which ordinary don't be developed in catabolic alkane reactions, in many cases have an inhibitory effect on alkane oxidationThe regulation of catabolic pathways has a great influence on the formation of specific products This influence is demonstrated examplarily at the production of biotin, fatty acids and citric acid.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020203
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  • 91
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    Practical aspects of the mathematical modeling of fermentation processes as a method of description, simulation, identification and optimizationPaper given at the 2nd Symposium of Socialist Countries on Biotechnology, Leipzig 2.-5. 12. 1980 (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 119-126 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Most mathematical models for describing the physiological state in fermentations lead to solutions of the so-called “stiff differential systems” during simulation on a digital computerThere is no suitable conventional software for solving these systemsAs a result of a relatively extensive screening of suitable methods for the solution of “stiff” differential systems (about 200 methods) it may be concluded that the semiimplicit RUNGE-KUTTA-formulas of the ROSENBROCK type, which constitute a part of the collection of programmes STIFFSOLVER-80, are optimal for the simulation of fermentation processesFor determining kinetic parameters from integral data the authors use the system of programmes BIOKINTheir practical application is discussed for 3 examples: 1Growth of the yeast Saccharomyces cerevisiae and changes in the content of specific compounds (proteins and ergosterol)2Quantitative evaluation of “;direct oxygen transfer” in the submerged culture.3Biosynthesis of a new antibiotic substance mucidin.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370020202
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  • 92
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    The blue light syndrome. Springer-Verlag, Berlin-Heidelberg-New York (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 178-178 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020210
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  • 93
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    Immobilization of β-glucosidase on an inorganic carrier (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 179-185 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The enzymatic hydrolysis of cellobiose, an important intermediate of the decomposition of cellulose containing materials, with immobilized β-glucosidase preparations from Geotrichium candidum, Trichoderma lignorum and Aspergillus foetidus was examinedAt first it was the aim to prepare from differently purified samples with different specific cellobiase activities high active preparations on the basis of the inorganic carrier Silochrom S-80. Characteristics e.g. thermal stability and temperature and pH optimum of immobilized preparations were compared with those of soluble preparationsKinetics of cellobiose hydrolysis by immobilized enzyme preparations were studied.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020211
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  • 94
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    Der Einfluß der Flockenbildung auf die Versorgung der Hefezellen mit Sauerstoff bei der Fermentation flüssiger Kohlenwasserstoffe (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 43-50 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Floc formation, especially the influence of floe diameter variations on the total velocity of the process, was investigated in aerobic growth processes of yeast on the hydrocarbons of crude oil.The experimental results show that the diameter of the flocs is a function of the rheological properties of the fluids and the flow conditions.The floc diameter varies between 0,1 mm and a few millimeters. About 90% of the total yeast cells are situated in the interior of the flocs.Since oxygen must be transferred to all yeast cells their oxygen supply was studied.Thus, the yeast cells in the floc interior were not sufficiently supplied with oxygen, if the floc diameter reached a critical value.In such cases a decrease of the biomass formation rate was observed, although the dissolved oxygen concentration of the aquaeous fermentation medium was greater than zero. Therefore, aerobic microbial growth processes in multicomponent systems must be carried out without floc formation or under such conditions as cause very small floc diameters.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020104
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  • 95
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    Some aspects of fermentation as an unit process in chemical engineering (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 59-71 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are described possibilities of scale up in stirred tank fermenters being based on known relations of mass-, energy-and heat transfer. The represented mathematical description shows the problems of the necessary scale up in the practice with the theory of similarity in the stirred tank fermenter. Relating on the method of RUSHTON for calculation of the energy capacity in different stirred fermenters the influence of the aeration is examined and confirmed with experimental results of the author. By the sysem of equations the known parameters of the aerobic fermenters can be calculated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020106
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  • 96
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    Effektivität von Modellierung und Optimierung mikrobieller Prozesse (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 73-77 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aim of the technological treatment of microbial processes is the optimal working of the production plant. The treatment itself is to be seen as a process whose realization requires a certain amount of expenditure. The problem of optimization of this process can with certain restictions be approximately solved in a series of steps. After a general formulation of the problem explanations are given for two important and typical steps - the determination of the extend of modelling and the choice of production cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020108
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  • 97
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    Regulation of lysine biosynthesis in Brevibacterium flavum (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 79-85 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: L-lysine synthesis pathway enzyme activities: β-aspartate kinase (EC.2.7.2.4), diaminopimelate decarboxylase (EC.4.1.1.20) for two L-lysine producing strains Brevibacterium flavum 22LD and RC-115 were studied. It has been found that β-aspartate kinase and diaminopimelate decarboxylase in the Br. flavum RC-115 are less sensitive to feed-back inhibition by lysine and threonine. It is supposed that desensitized β-aspartate kinase in the Br. flavum RC-115 can be determined by genetical changes of the regulatory properties of the β-aspartate kinase.Auxotrophity in the locus of homoserine dehydrogenase was tested and no homoserine dehydrogenase (EC.1.1.1.3) activity was found in either strain.The combination of these both types of mutation supplemented by the lack of catabolic repression in the RC-115 strain makes it an active lysine producer in the medium with high carbohydrates content.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020110
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  • 98
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    Erzeugung eines zellwandlytischen Enzymkomplexes aus Arthrobacter GJM-I zur Herstellung von Protoplasten aus Candia spec. H (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 95-102 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growing conditions have been found out for the bacterium Arthrobacter GJM-I to produce a lytic enzyme system, which converts cells of the yeast Candida spec. H to protoplasts quickly and in a good yield. Estimating the activities of α-mannanase and β-glucanase we found out the optimal culture time to gain the lytic enzyme system from the culture filtrate. It was shown that radioactive labeling of the yeast cells makes it possible to estimate quantitatively the conversion to protoplasts and the simultaneous lysis. The obtained lytic enzyme system can substitute the snail cnzyme system which was used for cell conversion of Candida spec. H to protoplasts till now.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020113
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  • 99
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    Calorimetric Glucose Determination by Glucose Oxidase  - Methylene Blue (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 103-106 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The reaction between glucose and methylene blue, catalyzed by glucose oxidase (GOD)was analysed calorimetrically.The amount of heat produced under saturating methylene blue concentrations ( 〉 10-2 mol/1)was measured with glucose concentration and time as parameters (kinetic procedure) Kinetic constants (pseudo one substrate kinetics) were derived from the experimental data: KM(glucose)= 1.18 × 10-3 mol/l and Vmax = 0.085 J/mg GOD min (3.89 · 10-6 mol/mg GOD min)Comparison of caloric with optical measurements gave an enthalpy of reaction of 22.52 kJ/mol. Considering the observed substrate inhibition, glucose determinations are possible up to glucose concentrations of 0.1 mol/l.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020114
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  • 100
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    Ethanol from Zymomonas, a bacterium (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 307-315 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Thirteen isolates ofZymomonas were analyzed for their ability to tolerate increasing concentrations of glucose and ethanol. In medium containing 5.0% (v/v) ethanol, four isolates grew well in 15.0% (w/v) glucose. Six cultures tolerated at least 6,0% ethanol. Of all the isolates, 7 preferred glucose and 4 preferred sucrose as a sugar substrate. In a nutrient medium containing mineral salts and high concentrations of pantothenate and biotin ethanol production for 2 isolates was approximately 7.0%. Continuous stirring and growth factors were responsible for this increased ethanol production.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020402
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